MD17G1261300.v1.1

Sorbitol dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0018083
Physical Location & Seq
Reverse (-)
32212317 .. 32212845
529 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1261300.v1.1.491

Sequence Viewer

Length: 390 bp
ATGAATATAGCTGCTTATGCTTCTGCTGGTGGCGGTGGTGGACCTGGTGGTGCTGCTGATGTCCACCCTTCTCGAGAAAAGTATGATGTGTTTAACACTATGGTTGCTTGGCTAGTTGATGTTAACACCATCAAGATCCTACCTTTCAAGCTCCCCACTATTGGACCGAATGATGTTCGAATTCGAATTAAGGATGTCAGCATTTGCGGAAGTGATTTTCACTACCTCAAGACCATGAAATGTGCGGATTTTGAGGTTAAAGAGCCGATGGTGATCGGACATAAGTGTGCTGGGATCGTAGACAAAGTTGGGAGCGAGGTGAAGCATTTGGTGCCCGGTGACAGAGTGGCGTTTGAGCCTAGCATCATCAGCTGCGCACATTGCCAGTAG

Protein Analysis

130

Amino Acids

13.84

Weight (kDa)

6.94

Isoelectric Point (pI)

29.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 55 - 128 2e-17 Alcohol dehydrogenase GroES-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018083)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g28900
malus_domestica MD00G1006100.v1.1 MD13G1239400.v1.1 MD17G1261300.v1.1
pyrus_communis pycom01g20510 pycom07g24110
rosa_multiflora Rmu_sc0016049.1_g000005
rosa_roxburghii Rroxscaffold_4G00282120
rosa_samantha Rh1AG407500 Rh1CG385000 Rh1DG402000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 376
AccB1I GGYRCC 1 cut(s) 331
AccI GTMKAC 1 cut(s) 300
AciI CCGC 3 cut(s) 33, 207, 245
AclWI GGATC 2 cut(s) 130, 302
AcsI RAATTY 1 cut(s) 180
AfiI CCNNNNNNNGG 1 cut(s) 161
AgsI TTSAA 1 cut(s) 148
AjnI CCWGG 1 cut(s) 43
AluBI AGCT 3 cut(s) 11, 151, 372
AluI AGCT 3 cut(s) 11, 151, 372
AlwI GGATC 2 cut(s) 130, 302
Ama87I CYCGRG 1 cut(s) 72
ApeKI GCWGC 3 cut(s) 11, 53, 372
ApoI RAATTY 1 cut(s) 180
AspLEI GCGC 1 cut(s) 377
AspS9I GGNCC 2 cut(s) 41, 164
AsuC2I CCSGG 1 cut(s) 336
AsuHPI GGTGA 3 cut(s) 283, 331, 350
AsuII TTCGAA 2 cut(s) 178, 184
AvaI CYCGRG 1 cut(s) 72
AvaII GGWCC 2 cut(s) 41, 164
BaeGI GKGCMC 1 cut(s) 336
BanI GGYRCC 1 cut(s) 331
BbvI GCAGC 2 cut(s) 40, 359
BccI CCATC 2 cut(s) 137, 262
BciT130I CCWGG 1 cut(s) 45
BcnI CCSGG 1 cut(s) 336
BfaI CTAG 2 cut(s) 113, 360
BisI GCNGC 3 cut(s) 12, 54, 373
BlsI GCNGC 3 cut(s) 13, 55, 374
Bme1390I CCNGG 2 cut(s) 45, 336
Bme18I GGWCC 2 cut(s) 41, 164
BmeT110I CYCGRG 1 cut(s) 72
BmgT120I GGNCC 2 cut(s) 41, 164
BmiI GGNNCC 1 cut(s) 333
BmrFI CCNGG 2 cut(s) 45, 336
BmsI GCATC 1 cut(s) 372
Bpu14I TTCGAA 2 cut(s) 178, 184
BpuEI CTTGAG 1 cut(s) 212
BpuMI CCSGG 1 cut(s) 336
BsaBI GATNNNNATC 1 cut(s) 272
Bsc4I CCNNNNNNNGG 1 cut(s) 161
Bse1I ACTGG 1 cut(s) 385
Bse3DI GCAATG 1 cut(s) 379
Bse8I GATNNNNATC 1 cut(s) 272
BseBI CCWGG 1 cut(s) 45
BseGI GGATG 1 cut(s) 199
BseJI GATNNNNATC 1 cut(s) 272
BseLI CCNNNNNNNGG 1 cut(s) 161
BseMI GCAATG 1 cut(s) 379
BseNI ACTGG 1 cut(s) 385
BseSI GKGCMC 1 cut(s) 336
BseXI GCAGC 2 cut(s) 40, 359
BseYI CCCAGC 1 cut(s) 290
BshNI GGYRCC 1 cut(s) 331
BsiHKCI CYCGRG 1 cut(s) 72
BsiSI CCGG 1 cut(s) 336
BslI CCNNNNNNNGG 1 cut(s) 161
BsoBI CYCGRG 1 cut(s) 72
Bsp119I TTCGAA 2 cut(s) 178, 184
Bsp1286I GDGCHC 1 cut(s) 336
Bsp143I GATC 3 cut(s) 135, 273, 294
BspACI CCGC 3 cut(s) 33, 207, 245
BspLI GGNNCC 1 cut(s) 333
BspPI GGATC 2 cut(s) 130, 302
BspT104I TTCGAA 2 cut(s) 178, 184
BspT107I GGYRCC 1 cut(s) 331
BsrDI GCAATG 1 cut(s) 379
BsrI ACTGG 1 cut(s) 385
BssMI GATC 3 cut(s) 135, 273, 294
Bst2UI CCWGG 1 cut(s) 45
BstAPI GCANNNNNTGC 1 cut(s) 331
BstBI TTCGAA 2 cut(s) 178, 184
BstF5I GGATG 1 cut(s) 199
BstHHI GCGC 1 cut(s) 377
BstKTI GATC 3 cut(s) 138, 276, 297
BstMBI GATC 3 cut(s) 135, 273, 294
BstMWI GCNNNNNNNGC 4 cut(s) 17, 331, 369, 381
BstNI CCWGG 1 cut(s) 45
BstSCI CCNGG 2 cut(s) 43, 334
BstSLI GKGCMC 1 cut(s) 336
BstV1I GCAGC 2 cut(s) 40, 359
BstX2I RGATCY 1 cut(s) 135
BstYI RGATCY 1 cut(s) 135
BtsCI GGATG 1 cut(s) 199
CfoI GCGC 1 cut(s) 377
Cfr13I GGNCC 2 cut(s) 41, 164
CsiI ACCWGGT 1 cut(s) 43
CviAII CATG 1 cut(s) 235
CviJI RGCY 6 cut(s) 11, 112, 151, 265, 358, 372
CviKI_1 RGCY 6 cut(s) 11, 112, 151, 265, 358, 372
DpnI GATC 3 cut(s) 137, 275, 296
DpnII GATC 3 cut(s) 135, 273, 294
Eco47I GGWCC 2 cut(s) 41, 164
Eco88I CYCGRG 1 cut(s) 72
EcoRI GAATTC 1 cut(s) 180
EcoRII CCWGG 1 cut(s) 43
FaeI CATG 1 cut(s) 238
FaiI YATR 6 cut(s) 8, 18, 84, 101, 236, 282
FatI CATG 1 cut(s) 234
FblI GTMKAC 1 cut(s) 300
Fnu4HI GCNGC 3 cut(s) 12, 54, 373
FokI GGATG 1 cut(s) 206
Fsp4HI GCNGC 3 cut(s) 12, 54, 373
FspBI CTAG 2 cut(s) 113, 360
FspI TGCGCA 1 cut(s) 376
GlaI GCGC 1 cut(s) 376
GluI GCNGC 3 cut(s) 12, 54, 373
GsaI CCCAGC 1 cut(s) 294
HapII CCGG 1 cut(s) 336
HhaI GCGC 1 cut(s) 377
Hin1II CATG 1 cut(s) 238
Hin6I GCGC 1 cut(s) 375
HinP1I GCGC 1 cut(s) 375
HincII GTYRAC 1 cut(s) 124
HindII GTYRAC 1 cut(s) 124
HpaI GTTAAC 1 cut(s) 124
HpaII CCGG 1 cut(s) 336
HphI GGTGA 3 cut(s) 283, 331, 350
Hpy166II GTNNAC 4 cut(s) 41, 64, 124, 301
Hpy188I TCNGA 1 cut(s) 278
Hpy188III TCNNGA 4 cut(s) 72, 74, 133, 229
Hpy8I GTNNAC 4 cut(s) 41, 64, 124, 301
HpyAV CCTTC 1 cut(s) 78
HpyF10VI GCNNNNNNNGC 4 cut(s) 17, 331, 369, 381
Hsp92II CATG 1 cut(s) 238
HspAI GCGC 1 cut(s) 375
KspAI GTTAAC 1 cut(s) 124
Kzo9I GATC 3 cut(s) 135, 273, 294
LmnI GCTCC 2 cut(s) 156, 312
LpnPI CCDG 5 cut(s) 12, 30, 57, 276, 349
Lsp1109I GCAGC 2 cut(s) 40, 359
LweI GCATC 1 cut(s) 372
MabI ACCWGGT 1 cut(s) 43
MaeI CTAG 2 cut(s) 113, 360
MaeIII GTNAC 1 cut(s) 338
MalI GATC 3 cut(s) 137, 275, 296
MboI GATC 3 cut(s) 135, 273, 294
MflI RGATCY 1 cut(s) 135
MhlI GDGCHC 1 cut(s) 336
MluCI AATT 2 cut(s) 180, 186
MnlI CCTC 3 cut(s) 236, 247, 310
MseI TTAA 4 cut(s) 93, 123, 189, 258
MslI CAYNNNNRTG 1 cut(s) 285
MspA1I CMGCKG 1 cut(s) 372
MspI CCGG 1 cut(s) 336
MspR9I CCNGG 2 cut(s) 45, 336
MvaI CCWGG 1 cut(s) 45
MwoI GCNNNNNNNGC 4 cut(s) 17, 331, 369, 381
NciI CCSGG 1 cut(s) 336
NdeII GATC 3 cut(s) 135, 273, 294
NlaIII CATG 1 cut(s) 238
NlaIV GGNNCC 1 cut(s) 333
NmuCI GTSAC 1 cut(s) 338
NsbI TGCGCA 1 cut(s) 376
NspV TTCGAA 2 cut(s) 178, 184
PaeR7I CTCGAG 1 cut(s) 72
PkrI GCNGC 3 cut(s) 13, 55, 374
Psp6I CCWGG 1 cut(s) 43
PspFI CCCAGC 1 cut(s) 290
PspGI CCWGG 1 cut(s) 43
PspN4I GGNNCC 1 cut(s) 333
PspPI GGNCC 2 cut(s) 41, 164
PsuI RGATCY 1 cut(s) 135
PvuII CAGCTG 1 cut(s) 372
RseI CAYNNNNRTG 1 cut(s) 285
SaqAI TTAA 4 cut(s) 93, 123, 189, 258
SatI GCNGC 3 cut(s) 12, 54, 373
Sau3AI GATC 3 cut(s) 135, 273, 294
Sau96I GGNCC 2 cut(s) 41, 164
ScrFI CCNGG 2 cut(s) 45, 336
SduI GDGCHC 1 cut(s) 336
SetI ASST 8 cut(s) 13, 46, 145, 153, 228, 258, 321, 374
SexAI ACCWGGT 1 cut(s) 43
SfaNI GCATC 1 cut(s) 372
Sfr274I CTCGAG 1 cut(s) 72
SfuI TTCGAA 2 cut(s) 178, 184
SinI GGWCC 2 cut(s) 41, 164
SlaI CTCGAG 1 cut(s) 72
SmiMI CAYNNNNRTG 1 cut(s) 285
SmlI CTYRAG 2 cut(s) 72, 227
SmoI CTYRAG 2 cut(s) 72, 227
Sse9I AATT 2 cut(s) 180, 186
SsiI CCGC 3 cut(s) 33, 207, 245
SspMI CTAG 2 cut(s) 113, 360
StyD4I CCNGG 2 cut(s) 43, 334
TaqI TCGA 3 cut(s) 73, 178, 184
TaqII GACCGA 1 cut(s) 181
TasI AATT 2 cut(s) 180, 186
Tru1I TTAA 4 cut(s) 93, 123, 189, 258
Tru9I TTAA 4 cut(s) 93, 123, 189, 258
TseFI GTSAC 1 cut(s) 338
TseI GCWGC 3 cut(s) 11, 53, 372
Tsp45I GTSAC 1 cut(s) 338
TspDTI ATGAA 2 cut(s) 17, 251
VpaK11BI GGWCC 2 cut(s) 41, 164
XapI RAATTY 1 cut(s) 180
XhoI CTCGAG 1 cut(s) 72
XmiI GTMKAC 1 cut(s) 300
XspI CTAG 2 cut(s) 113, 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.