Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0005890

Physical Location & Seq

Genomic Coordinates Reverse (-)

1072406 .. 1078637

Gene Structure 6232 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1008200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3360 bp

ATGACCAGGGCTGTTCGCAATAGGATACTCAAGGATGCGAATGGTGATACTAGTGATCATCTGCGCAACCACATTCATTTGACAAATTGCATTCACTTGAAGAACCATATGCACAAGCAAAGCCCCATATTGGCGGACAGGTCACTTATGAGGGACCTTGTCGTCCTTCAGAGGTCGCGATCTCTCAGGGACCCTTCTGCCAGTCCGCCCTCATGGCATTCGCCTTCCATTCTTGATATGCTTTCCAGAAGAGGTGAAAATGATGCCTTGGTGCGTGAGGGGAGAAGGTCAGTAGGAAGTGAGTATCGAAGGGATGCCAGGAGGCTATCAGCAGGTTCTCCACCTTTGGCAAGTTTAGCGACACCAAAAGTTGCTCAACGTGAGGCTAATGCGGGAAATGATGGTGTGGCCGGAACCAGTGAACATGGAAGCAAGAGTGGAGTTAGGGATGGTGGGAAAATTAGGAGAGAAGAATCTAGTCAGAAGAGTAATAGAAGTGAAAGTTTGGGTGGCAATGAGGAGCCTCCGCTAGACCAGAATGGTAACGATTTGACTCATGACGCTCTTTCTGGGAATTCAGAATCCAAAATTAGAAGAAGTAAACAAAAGGGAAAGCACACCCAAGGTGCTCAAATGAAGACTCTCTCAGAGCAATTGAATGATGTTAGGATGGATACTGATGATGTAGCGTCCTCTAATATCCACCTGCCTGGAAGACGGTCCAGGCAGGGAAGAATCTCTGAGGAACAGGAGGTCAGCGCTCGGGGCTATTGCAGCGGACTAAGTAGGGTAAAAAGGCGGAGATTTCGAGGTGCAAGAAGAAGCCGTGCTTCTGTAGCGTCCAGAGACTTTGGAGCTCAGCATGGTTTATCTGTAGCTTCTAATTCTATAGCTCAAGGGTCAGCTCACCGAAAGTATCACATGGAACAGGGAGCGGATGAGTATGGTGAGCAAAATGTCACAAGGGCTCCTAGAAACGGGTGTGGGATTCCCTGGAATTGGTCGAGAATCCATCATAGAGGTAAAACATTCCTTGACATTGCTGGAAGGAGCATTTCTTGTGGTTTATCTGATTCAAGGTTTAAGAAGGGTGATCTGGCTGCCCATGGTAGAGATATTTCTGACATGCCCATGGCAACTGATAACTCGAGCGTTTCTACTAAATCTGAACCTGAGGCACTTCCTTTACTAGTTGAAGCATCTGGATCACAGGCAAGCTCTGACAATGCTGGTTGGGTACATGACTATTCTGGTGAACTTGGTATTTATGCAGATAATTTATTCAAGCATGATGTCGGTTCCGACTATGCTTCTGAAGCTAGATCTGGTGATCAACGCAAATTGAGGGGACACCGCCGTCGTAGGCACCAAAATCTGACGCAAAAATACATGCCGAGAACCTTTAGAGATCTTGTGGGGCAGAATTTGGTGGCACAAGCTCTTTCAAATGCTGTCATGAAAAAGAAAGTTGGGTTGCTATACGTGTTCTATGGTCCTCATGGCACAGGGAAAACATCATGTGCTCGCATATTTTCTAGAGCTCTAAATTGCCAGTCTCTGGATCATCCCAAGCCTTGTGGCTTTTGCAATTCTTGCATTGCACATGATATGGGCAACAGTAGAAATATAAAGGAAGTTGGGCCTGTTAGTAACTTCGACTTCGATAGCATTATGGACCTACTTGACAATATGAGTATTTCCCAACTGCCATCACAGTGCAGAGTGTTTATCTTTGATGATTGTGAAACATTGTCCCCTGAGTGCTGGAGTGCCTTATCGAAGGTCATTGATCGAGATGCAGATATTATTTACAGTTTGCAATGGATAGCAGCCAAAGAAGATATAGAAATTGACAAGGATGCACTGAAACTTATTTCGTCAAGGTCGGATGGTTCATTGAGGGATGCTGAGATGACTCTAGAGCAACTGAGTTTGCTTGGCCAGAGAATCTCAGTTCCTCTTGTTCAGGAACTGGTTGGGCTTATATCTGATGAGAAATTGATAGATCTTCTTGATTTAGCATTATCTGCAGACACAGTTAATACTGTGAAGAATTTGAGAATGATAATGGAAACTGGAGTTGAGCCATTAACTTTGATGTCACAACTTGCAACAGTAATTACAGATATACTTGCTGGCAGCTATGATTATAGAAAAGAAAGGCGTAGAAGGAAGTTCTTTCGGAACCAACCATTATCAAAAGAAGACATGGAGAAGTTGCGTCAAGCTTTGAAAACTTTATCTGAGGCCGAGAAACAATTGAGGATGTCAAATGACAAGTTAACATGGCTAACCGCTGCACTTCTCCAACTTGCTCCTGATCAACAGTATATGTTGCCTAGTTCTTCTGGAGGCACAAGTTTCCATCAGAGTCCGTTGGCCTTAAATAATGTGAGCGCAAGGGATGCGGTCAGGAGAGATAGTGAACAAGATGAGATGCCAAATTATGAGAAAGGCTTGCCAGCAAATGCTCGAAATTCTGTAAGTTCTGGAAAAGGTATGAGTTTAGATAGAAAACGTCATGCTGGGATGGCTATGGCTTCTCAGCAGATGGCTACAAGCTCCGCTGAAATAACCAGGGTAAATGGGAGGCAAATCCATGGCAAAAGCCACAAAGGAATTGAAGAAATTTGGTTGGAGGTGCTTGAGAAAATTCCGTATAATCGTGTAAAAGAATTTTTGTACCAAGAAGGGAAGCTGACCTCGGTTAGTTTCGGTGCAGCACCCACTGCGCAGTTGATGTTCTCTTCGCATATGACCAAATCTACCGCAGAGAAGTTCAGAGCGCAGATTTTGCAGGCATTTGAGATTGTTCTAGGGTCTCCCCTGACAATTGAGATTAGATGTGAATCAAAAAGAAATAGTAAAGAATGGGCCCAGATGCCTATTATTATACCAGCTTCAAAGGATGGTTCATCCCAGATTAGAGATGAAAATACGTTCAGCGCCCGTGAAGTGGGGACGAGTGAAATTGTTGAAGTAGCTGCTTCTCCTAGGGAAAGCAAAGGTAGTGGTCAAATGCATAACCAAAAGAAACCAACCATGGCGATAATTCCAGAAAAACAACAAAGCCAGAGTCAAAGCATTGTTAGGAGCAAGGTGTCCCTTGCACATGTACTTCAGCACTCGGAGTTGCAACGTGGATGGTCACAACGTAAAGCAGTTTCTATTGCTGAAAAGCTCGAACAAGATAATTTGAGACTGGAATCTCGATCGAGAAGTTTGCTTTGCTGGAAAGCGTCTAGAGTAACTCGTCGAAGGCTTTCGCGGTTGAAGATAAGGGCACGAAGACCGCATTCATTGCTGAAGCTCGTGTCCTGCGGAAAGTGTCTCTCTTCAAGGTCACCAAGGGCAGTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005663 GO:0005694 GO:0005886 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0016020 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0046483 GO:0050896 GO:0051716 GO:0071704 GO:0071944 GO:0090304 GO:1901360 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DNA_pol3_delta2 DNA_pol3_gamma3

Protein Analysis

1120

Amino Acids

124.54

Weight (kDa)

9.66

Isoelectric Point (pI)

61.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_pol3_delta2	PF13177	474 - 597	3.6e-15	DNA polymerase III, delta subunit
DNAX_ATPase_lid	PF22608	604 - 650	8.6e-06	DNA polymerase III clamp loader subunit, ATPase lid domain
DNA_pol3_gamma3	PF12169	653 - 773	7.5e-06	DNA polymerase III subunits gamma and tau domain III
DnaA_N-like_STI	PF23007	868 - 949	1.1e-27	STICHEL, DnaA_N-like alpha-beta domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0005890)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G18820
fragaria_vesca	FvH4_3g07230 FvH4_3g07230 FvH4_3g07230 FvH4_3g07230 FvH4_3g07230 FvH4_3g07230 FvH4_3g07230 FvH4_3g07230
malus_domestica	MD00G1008200.v1.1 MD10G1274700.v1.1
prunus_persica	Prupe.4G067700_v2.0.a1 Prupe.4G067700_v2.0.a1 Prupe.4G067700_v2.0.a1 Prupe.4G067700_v2.0.a1
pyrus_communis	pycom05g27350 pycom10g22920
rosa_chinensis	RchiOBHm_Chr5g0011921
rosa_laevigata	RLG00000031869
rosa_multiflora	Rmu_sc0001475.1_g000004
rosa_roxburghii	Rroxscaffold_1G00064670
rosa_rugosa	Rorug04G0460000 Rorug04G0460100
rosa_samantha	Rh5AG091500 Rh5BG087300 Rh5CG101200 Rh5DG087900
rosa_wichuraiana	Rw5G007980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	714
AasI	GACNNNNNNGTC	2 cut(s)	161, 1356
Acc16I	TGCGCA	2 cut(s)	65, 2733
Acc36I	ACCTGC	2 cut(s)	323, 714
AccB1I	GGYRCC	1 cut(s)	1365
AccB7I	CCANNNNNTGG	1 cut(s)	1558
AccBSI	CCGCTC	1 cut(s)	935
AccII	CGCG	2 cut(s)	178, 3268
AclWI	GGATC	2 cut(s)	1213, 1569
AcoI	YGGCCR	2 cut(s)	408, 1939
AcsI	RAATTY	7 cut(s)	574, 1423, 2053, 2476, 2628, 2652, 2675
AcuI	CTGAAG	4 cut(s)	152, 1335, 3104, 3326
AfaI	GTAC	3 cut(s)	1239, 2684, 3117
AfeI	AGCGCT	1 cut(s)	760
AfiI	CCNNNNNNNGG	5 cut(s)	130, 977, 999, 1558, 2956
AflIII	ACRYGT	2 cut(s)	1482, 3112
AhlI	ACTAGT	2 cut(s)	50, 1189
AjnI	CCWGG	6 cut(s)	5, 317, 709, 722, 992, 2576
Alw21I	GWGCWC	4 cut(s)	631, 859, 1525, 1543
Alw26I	GTCTC	5 cut(s)	840, 1560, 2826, 3193, 3335
AlwI	GGATC	2 cut(s)	1213, 1569
AlwNI	CAGNNNCTG	2 cut(s)	1558, 1972
Ama87I	CYCGRG	2 cut(s)	762, 1147
Aor51HI	AGCGCT	1 cut(s)	760
AoxI	GGCC	6 cut(s)	408, 1640, 1939, 2247, 2379, 2874
ApaI	GGGCCC	1 cut(s)	2878
ApeKI	GCWGC	7 cut(s)	774, 1100, 1829, 2139, 2297, 2720, 2984
ApoI	RAATTY	7 cut(s)	574, 1423, 2053, 2476, 2628, 2652, 2675
ArsI	GACNNNNNNTTYG	2 cut(s)	3061, 3093
Asp700I	GAANNNNTTC	1 cut(s)	3262
AspA2I	CCTAGG	1 cut(s)	2993
AspLEI	GCGC	6 cut(s)	66, 761, 2399, 2734, 2788, 2948
AspS9I	GGNCC	8 cut(s)	154, 190, 720, 1493, 1640, 1675, 2874, 2875
AsuHPI	GGTGA	8 cut(s)	56, 266, 899, 959, 1103, 1265, 1340, 3336
AvaI	CYCGRG	2 cut(s)	762, 1147
AvaII	GGWCC	5 cut(s)	154, 190, 720, 1493, 1675
AvrII	CCTAGG	1 cut(s)	2993
AxyI	CCTNAGG	1 cut(s)	1173
BaeGI	GKGCMC	2 cut(s)	2878, 3286
BalI	TGGCCA	1 cut(s)	1941
BanI	GGYRCC	1 cut(s)	1365
BanII	GRGCYC	4 cut(s)	859, 970, 1543, 2878
BauI	CACGAG	1 cut(s)	3311
BbsI	GAAGAC	4 cut(s)	644, 721, 2211, 3295
Bbv12I	GWGCWC	4 cut(s)	631, 859, 1525, 1543
BbvI	GCAGC	7 cut(s)	786, 1087, 1841, 2151, 2284, 2732, 2971
BceAI	ACGGC	2 cut(s)	810, 1341
BcgI	CGANNNNNNTGC	2 cut(s)	3205, 3239
BciT130I	CCWGG	6 cut(s)	7, 319, 711, 724, 994, 2578
BciVI	GTATCC	2 cut(s)	18, 667
BclI	TGATCA	3 cut(s)	55, 1330, 2320
BcoDI	GTCTC	5 cut(s)	840, 1560, 2826, 3193, 3335
BcuI	ACTAGT	2 cut(s)	50, 1189
BfmI	CTRYAG	4 cut(s)	834, 873, 888, 2028
BfoI	RGCGCY	2 cut(s)	762, 2949
BfuAI	ACCTGC	2 cut(s)	323, 714
BfuI	GTATCC	2 cut(s)	18, 667
BglI	GCCNNNNNGGC	1 cut(s)	214
BglII	AGATCT	3 cut(s)	1322, 1408, 2005
BisI	GCNGC	7 cut(s)	775, 1101, 1830, 2140, 2298, 2721, 2985
BlnI	CCTAGG	1 cut(s)	2993
BlpI	GCTNAGC	1 cut(s)	858
BlsI	GCNGC	7 cut(s)	776, 1102, 1831, 2141, 2299, 2722, 2986
Bme1390I	CCNGG	6 cut(s)	7, 319, 711, 724, 994, 2578
Bme18I	GGWCC	5 cut(s)	154, 190, 720, 1493, 1675
BmeT110I	CYCGRG	2 cut(s)	762, 1147
BmgT120I	GGNCC	8 cut(s)	154, 190, 720, 1493, 1640, 1675, 2874, 2875
BmrFI	CCNGG	6 cut(s)	7, 319, 711, 724, 994, 2578
BpiI	GAAGAC	4 cut(s)	644, 721, 2211, 3295
BpmI	CTGGAG	3 cut(s)	1786, 2097, 2370
Bpu1102I	GCTNAGC	1 cut(s)	858
BpuEI	CTTGAG	3 cut(s)	14, 879, 2666
BsaAI	YACGTR	1 cut(s)	1483
BsaBI	GATNNNNATC	1 cut(s)	2848
BsaI	GGTCTC	1 cut(s)	2826
BsaXI	ACNNNNNCTCC	4 cut(s)	952, 982, 2407, 2437
Bsc4I	CCNNNNNNNGG	5 cut(s)	130, 977, 999, 1558, 2956
Bse1I	ACTGG	6 cut(s)	201, 417, 1552, 1977, 2080, 3207
Bse21I	CCTNAGG	1 cut(s)	1173
Bse3DI	GCAATG	5 cut(s)	520, 1038, 1596, 1826, 3299
Bse8I	GATNNNNATC	1 cut(s)	2848
BseBI	CCWGG	6 cut(s)	7, 319, 711, 724, 994, 2578
BseJI	GATNNNNATC	1 cut(s)	2848
BseLI	CCNNNNNNNGG	5 cut(s)	130, 977, 999, 1558, 2956
BseMI	GCAATG	5 cut(s)	520, 1038, 1596, 1826, 3299
BseNI	ACTGG	6 cut(s)	201, 417, 1552, 1977, 2080, 3207
BseRI	GAGGAG	1 cut(s)	533
BseSI	GKGCMC	2 cut(s)	2878, 3286
BseXI	GCAGC	7 cut(s)	786, 1087, 1841, 2151, 2284, 2732, 2971
BseYI	CCCAGC	1 cut(s)	2525
BsgI	GTGCAG	3 cut(s)	1738, 2283, 2739
Bsh1236I	CGCG	2 cut(s)	178, 3268
Bsh1285I	CGRYCG	1 cut(s)	3215
BshFI	GGCC	6 cut(s)	410, 1642, 1941, 2249, 2381, 2876
BshNI	GGYRCC	1 cut(s)	1365
BsiEI	CGRYCG	1 cut(s)	3215
BsiHKAI	GWGCWC	4 cut(s)	631, 859, 1525, 1543
BsiHKCI	CYCGRG	2 cut(s)	762, 1147
BsiSI	CCGG	1 cut(s)	411
BslFI	GGGAC	6 cut(s)	167, 203, 1362, 1738, 2974, 3088
BslI	CCNNNNNNNGG	5 cut(s)	130, 977, 999, 1558, 2956
BsmAI	GTCTC	5 cut(s)	840, 1560, 2826, 3193, 3335
BsmFI	GGGAC	6 cut(s)	167, 203, 1362, 1738, 2974, 3088
BsmI	GAATGC	3 cut(s)	90, 217, 3295
BsnI	GGCC	6 cut(s)	410, 1642, 1941, 2249, 2381, 2876
Bso31I	GGTCTC	1 cut(s)	2826
BsoBI	CYCGRG	2 cut(s)	762, 1147
Bsp120I	GGGCCC	1 cut(s)	2874
Bsp1286I	GDGCHC	7 cut(s)	631, 859, 970, 1525, 1543, 2878, 3286
Bsp1720I	GCTNAGC	1 cut(s)	858
Bsp19I	CCATGG	4 cut(s)	1105, 1131, 2599, 3042
Bsp68I	TCGCGA	1 cut(s)	178
BspANI	GGCC	6 cut(s)	410, 1642, 1941, 2249, 2381, 2876
BspFNI	CGCG	2 cut(s)	178, 3268
BspHI	TCATGA	2 cut(s)	556, 1455
BspMAI	CTGCAG	1 cut(s)	2032
BspMI	ACCTGC	2 cut(s)	323, 714
BspPI	GGATC	2 cut(s)	1213, 1569
BspT107I	GGYRCC	1 cut(s)	1365
BspTNI	GGTCTC	1 cut(s)	2826
BsrBI	CCGCTC	1 cut(s)	935
BsrDI	GCAATG	5 cut(s)	520, 1038, 1596, 1826, 3299
BsrI	ACTGG	6 cut(s)	201, 417, 1552, 1977, 2080, 3207
BssSI	CACGAG	1 cut(s)	3311
BssT1I	CCWWGG	8 cut(s)	267, 622, 1105, 1131, 2599, 2993, 3042, 3347
Bst2BI	CACGAG	1 cut(s)	3311
Bst2UI	CCWGG	6 cut(s)	7, 319, 711, 724, 994, 2578
Bst4CI	ACNGT	8 cut(s)	720, 1619, 1716, 1814, 2038, 2047, 2116, 2328
Bst6I	CTCTTC	4 cut(s)	244, 479, 2752, 3340
BstAPI	GCANNNNNTGC	6 cut(s)	1593, 2027, 2405, 2729, 2794, 3301
BstBAI	YACGTR	1 cut(s)	1483
BstC8I	GCNNGC	6 cut(s)	1216, 1525, 2137, 2459, 2463, 2799
BstDSI	CCRYGG	4 cut(s)	1105, 1131, 2599, 3042
BstEII	GGTNACC	1 cut(s)	3342
BstFNI	CGCG	2 cut(s)	178, 3268
BstH2I	RGCGCY	2 cut(s)	762, 2949
BstHHI	GCGC	6 cut(s)	66, 761, 2399, 2734, 2788, 2948
BstMAI	GTCTC	5 cut(s)	840, 1560, 2826, 3193, 3335
BstMCI	CGRYCG	1 cut(s)	3215
BstNI	CCWGG	6 cut(s)	7, 319, 711, 724, 994, 2578
BstNSI	RCATGY	3 cut(s)	1129, 1393, 3116
BstPI	GGTNACC	1 cut(s)	3342
BstSCI	CCNGG	6 cut(s)	5, 317, 709, 722, 992, 2576
BstSFI	CTRYAG	4 cut(s)	834, 873, 888, 2028
BstSLI	GKGCMC	2 cut(s)	2878, 3286
BstUI	CGCG	2 cut(s)	178, 3268
BstV1I	GCAGC	7 cut(s)	786, 1087, 1841, 2151, 2284, 2732, 2971
BstV2I	GAAGAC	4 cut(s)	644, 721, 2211, 3295
BstX2I	RGATCY	3 cut(s)	1322, 1408, 2005
BstXI	CCANNNNNNTGG	1 cut(s)	710
BstYI	RGATCY	3 cut(s)	1322, 1408, 2005
Bsu36I	CCTNAGG	1 cut(s)	1173
BsuI	GTATCC	2 cut(s)	18, 667
BsuRI	GGCC	6 cut(s)	410, 1642, 1941, 2249, 2381, 2876
BtgI	CCRYGG	4 cut(s)	1105, 1131, 2599, 3042
BtsI	GCAGTG	1 cut(s)	2727
BtsIMutI	CAGTG	4 cut(s)	424, 1721, 1862, 2727
BtuMI	TCGCGA	1 cut(s)	178
BveI	ACCTGC	2 cut(s)	323, 714
Cac8I	GCNNGC	6 cut(s)	1216, 1525, 2137, 2459, 2463, 2799
CaiI	CAGNNNCTG	2 cut(s)	1558, 1972
CciI	TCATGA	2 cut(s)	556, 1455
CfoI	GCGC	6 cut(s)	66, 761, 2399, 2734, 2788, 2948
Cfr13I	GGNCC	8 cut(s)	154, 190, 720, 1493, 1640, 1675, 2874, 2875
CseI	GACGC	6 cut(s)	569, 678, 828, 1387, 2210, 3228
Csp6I	GTAC	3 cut(s)	1238, 2683, 3116
CspCI	CAANNNNNGTGG	4 cut(s)	1558, 1593, 2992, 3027
CviQI	GTAC	3 cut(s)	1238, 2683, 3116
DrdI	GACNNNNNNGTC	2 cut(s)	161, 1356
DseDI	GACNNNNNNGTC	2 cut(s)	161, 1356
EaeI	YGGCCR	2 cut(s)	408, 1939
Eam1104I	CTCTTC	4 cut(s)	244, 479, 2752, 3340
EarI	CTCTTC	4 cut(s)	244, 479, 2752, 3340
EciI	GGCGGA	3 cut(s)	149, 195, 814
Ecl136II	GAGCTC	2 cut(s)	857, 1541
Eco130I	CCWWGG	8 cut(s)	267, 622, 1105, 1131, 2599, 2993, 3042, 3347
Eco24I	GRGCYC	4 cut(s)	859, 970, 1543, 2878
Eco31I	GGTCTC	1 cut(s)	2826
Eco47I	GGWCC	5 cut(s)	154, 190, 720, 1493, 1675
Eco47III	AGCGCT	1 cut(s)	760
Eco53kI	GAGCTC	2 cut(s)	857, 1541
Eco57I	CTGAAG	4 cut(s)	152, 1335, 3104, 3326
Eco81I	CCTNAGG	1 cut(s)	1173
Eco88I	CYCGRG	2 cut(s)	762, 1147
Eco91I	GGTNACC	1 cut(s)	3342
EcoICRI	GAGCTC	2 cut(s)	857, 1541
EcoO109I	RGGNCCY	2 cut(s)	154, 190
EcoO65I	GGTNACC	1 cut(s)	3342
EcoRI	GAATTC	1 cut(s)	574
EcoRII	CCWGG	6 cut(s)	5, 317, 709, 722, 992, 2576
EcoT14I	CCWWGG	8 cut(s)	267, 622, 1105, 1131, 2599, 2993, 3042, 3347
EcoT22I	ATGCAT	1 cut(s)	3024
EcoT38I	GRGCYC	4 cut(s)	859, 970, 1543, 2878
ErhI	CCWWGG	8 cut(s)	267, 622, 1105, 1131, 2599, 2993, 3042, 3347
FalI	AAGNNNNNCTT	2 cut(s)	814, 846
FaqI	GGGAC	6 cut(s)	167, 203, 1362, 1738, 2974, 3088
FauI	CCCGC	1 cut(s)	385
FauNDI	CATATG	2 cut(s)	108, 2754
FbaI	TGATCA	3 cut(s)	55, 1330, 2320
Fnu4HI	GCNGC	7 cut(s)	775, 1101, 1830, 2140, 2298, 2721, 2985
FriOI	GRGCYC	4 cut(s)	859, 970, 1543, 2878
Fsp4HI	GCNGC	7 cut(s)	775, 1101, 1830, 2140, 2298, 2721, 2985
FspI	TGCGCA	2 cut(s)	65, 2733
GlaI	GCGC	6 cut(s)	65, 760, 2398, 2733, 2787, 2947
GluI	GCNGC	7 cut(s)	775, 1101, 1830, 2140, 2298, 2721, 2985
GsaI	CCCAGC	1 cut(s)	2529
GsuI	CTGGAG	3 cut(s)	1786, 2097, 2370
HaeII	RGCGCY	2 cut(s)	762, 2949
HaeIII	GGCC	6 cut(s)	410, 1642, 1941, 2249, 2381, 2876
HapII	CCGG	1 cut(s)	411
HgaI	GACGC	6 cut(s)	569, 678, 828, 1387, 2210, 3228
HhaI	GCGC	6 cut(s)	66, 761, 2399, 2734, 2788, 2948
Hin6I	GCGC	6 cut(s)	64, 759, 2397, 2732, 2786, 2946
HinP1I	GCGC	6 cut(s)	64, 759, 2397, 2732, 2786, 2946
HincII	GTYRAC	1 cut(s)	2283
HindII	GTYRAC	1 cut(s)	2283
HindIII	AAGCTT	1 cut(s)	2226
HpaI	GTTAAC	1 cut(s)	2283
HpaII	CCGG	1 cut(s)	411
HphI	GGTGA	8 cut(s)	56, 266, 899, 959, 1103, 1265, 1340, 3336
Hpy166II	GTNNAC	5 cut(s)	422, 602, 1256, 2283, 2426
Hpy8I	GTNNAC	5 cut(s)	422, 602, 1256, 2283, 2426
Hpy99I	CGWCG	2 cut(s)	1362, 3258
HpyCH4III	ACNGT	8 cut(s)	720, 1619, 1716, 1814, 2038, 2047, 2116, 2328
HpyCH4IV	ACGT	6 cut(s)	379, 1482, 2518, 2939, 3139, 3154
HpySE526I	ACGT	6 cut(s)	379, 1482, 2518, 2939, 3139, 3154
HspAI	GCGC	6 cut(s)	64, 759, 2397, 2732, 2786, 2946
KflI	GGGWCCC	1 cut(s)	190
Ksp22I	TGATCA	3 cut(s)	55, 1330, 2320
KspAI	GTTAAC	1 cut(s)	2283
LmnI	GCTCC	8 cut(s)	520, 854, 932, 973, 1050, 2320, 2567, 3093
Lsp1109I	GCAGC	7 cut(s)	786, 1087, 1841, 2151, 2284, 2732, 2971
MaeII	ACGT	6 cut(s)	379, 1482, 2518, 2939, 3139, 3154
MaeIII	GTNAC	8 cut(s)	141, 542, 958, 1649, 2100, 3147, 3247, 3342
MbiI	CCGCTC	1 cut(s)	935
MfeI	CAATTG	3 cut(s)	653, 2258, 2832
MflI	RGATCY	3 cut(s)	1322, 1408, 2005
MhlI	GDGCHC	7 cut(s)	631, 859, 970, 1525, 1543, 2878, 3286
MlsI	TGGCCA	1 cut(s)	1941
MluNI	TGGCCA	1 cut(s)	1941
MlyI	GAGTC	5 cut(s)	547, 634, 1909, 2380, 3085
MmeI	TCCRAC	4 cut(s)	1326, 1866, 2332, 2616
Mox20I	TGGCCA	1 cut(s)	1941
Mph1103I	ATGCAT	1 cut(s)	3024
MroXI	GAANNNNTTC	1 cut(s)	3262
MscI	TGGCCA	1 cut(s)	1941
MseI	TTAA	5 cut(s)	1083, 2040, 2090, 2282, 2384
MslI	CAYNNNNRTG	2 cut(s)	1130, 1714
Msp20I	TGGCCA	1 cut(s)	1941
MspA1I	CMGCKG	3 cut(s)	777, 2297, 2567
MspI	CCGG	1 cut(s)	411
MspR9I	CCNGG	6 cut(s)	7, 319, 711, 724, 994, 2578
MunI	CAATTG	3 cut(s)	653, 2258, 2832
Mva1269I	GAATGC	3 cut(s)	90, 217, 3295
MvaI	CCWGG	6 cut(s)	7, 319, 711, 724, 994, 2578
MvnI	CGCG	2 cut(s)	178, 3268
NcoI	CCATGG	4 cut(s)	1105, 1131, 2599, 3042
NdeI	CATATG	2 cut(s)	108, 2754
NmeAIII	GCCGAG	2 cut(s)	1419, 2275
NmuCI	GTSAC	5 cut(s)	141, 958, 2100, 3147, 3342
NruI	TCGCGA	1 cut(s)	178
NsbI	TGCGCA	2 cut(s)	65, 2733
NsiI	ATGCAT	1 cut(s)	3024
NspI	RCATGY	3 cut(s)	1129, 1393, 3116
PaeR7I	CTCGAG	1 cut(s)	1147
PagI	TCATGA	2 cut(s)	556, 1455
PaqCI	CACCTGC	1 cut(s)	714
PciI	ACATGT	1 cut(s)	3112
PctI	GAATGC	3 cut(s)	90, 217, 3295
PdmI	GAANNNNTTC	1 cut(s)	3262
PfeI	GAWTC	9 cut(s)	473, 581, 735, 988, 1008, 1073, 1947, 2849, 3206
PflFI	GACNNNGTC	1 cut(s)	158
PflMI	CCANNNNNTGG	1 cut(s)	1558
PkrI	GCNGC	7 cut(s)	776, 1102, 1831, 2141, 2299, 2722, 2986
Ple19I	CGATCG	1 cut(s)	3215
PleI	GAGTC	5 cut(s)	547, 634, 1909, 2379, 3084
PpsI	GAGTC	5 cut(s)	547, 634, 1909, 2379, 3084
Ppu21I	YACGTR	1 cut(s)	1483
PpuMI	RGGWCCY	2 cut(s)	154, 190
PscI	ACATGT	1 cut(s)	3112
Psp124BI	GAGCTC	2 cut(s)	859, 1543
Psp5II	RGGWCCY	2 cut(s)	154, 190
Psp6I	CCWGG	6 cut(s)	5, 317, 709, 722, 992, 2576
PspEI	GGTNACC	1 cut(s)	3342
PspFI	CCCAGC	1 cut(s)	2525
PspGI	CCWGG	6 cut(s)	5, 317, 709, 722, 992, 2576
PspOMI	GGGCCC	1 cut(s)	2874
PspPI	GGNCC	8 cut(s)	154, 190, 720, 1493, 1640, 1675, 2874, 2875
PspPPI	RGGWCCY	2 cut(s)	154, 190
PspXI	VCTCGAGB	1 cut(s)	1147
PstI	CTGCAG	1 cut(s)	2032
PstNI	CAGNNNCTG	2 cut(s)	1558, 1972
PsuI	RGATCY	3 cut(s)	1322, 1408, 2005
PsyI	GACNNNGTC	1 cut(s)	158
PvuI	CGATCG	1 cut(s)	3215
RruI	TCGCGA	1 cut(s)	178
RsaI	GTAC	3 cut(s)	1239, 2684, 3117
RsaNI	GTAC	3 cut(s)	1238, 2683, 3116
RseI	CAYNNNNRTG	2 cut(s)	1130, 1714
SacI	GAGCTC	2 cut(s)	859, 1543
SaqAI	TTAA	5 cut(s)	1083, 2040, 2090, 2282, 2384
SatI	GCNGC	7 cut(s)	775, 1101, 1830, 2140, 2298, 2721, 2985
Sau96I	GGNCC	8 cut(s)	154, 190, 720, 1493, 1640, 1675, 2874, 2875
SchI	GAGTC	5 cut(s)	547, 634, 1909, 2380, 3085
ScrFI	CCNGG	6 cut(s)	7, 319, 711, 724, 994, 2578
SduI	GDGCHC	7 cut(s)	631, 859, 970, 1525, 1543, 2878, 3286
SfcI	CTRYAG	4 cut(s)	834, 873, 888, 2028
Sfr274I	CTCGAG	1 cut(s)	1147
SinI	GGWCC	5 cut(s)	154, 190, 720, 1493, 1675
SlaI	CTCGAG	1 cut(s)	1147
SmiMI	CAYNNNNRTG	2 cut(s)	1130, 1714
SmlI	CTYRAG	4 cut(s)	29, 894, 1147, 2645
SmoI	CTYRAG	4 cut(s)	29, 894, 1147, 2645
SpeI	ACTAGT	2 cut(s)	50, 1189
SstI	GAGCTC	2 cut(s)	859, 1543
StyD4I	CCNGG	6 cut(s)	5, 317, 709, 722, 992, 2576
StyI	CCWWGG	8 cut(s)	267, 622, 1105, 1131, 2599, 2993, 3042, 3347
TaaI	ACNGT	8 cut(s)	720, 1619, 1716, 1814, 2038, 2047, 2116, 2328
TaiI	ACGT	6 cut(s)	382, 1485, 2521, 2942, 3142, 3157
TatI	WGTACW	1 cut(s)	3115
TfiI	GAWTC	9 cut(s)	473, 581, 735, 988, 1008, 1073, 1947, 2849, 3206
Tru1I	TTAA	5 cut(s)	1083, 2040, 2090, 2282, 2384
Tru9I	TTAA	5 cut(s)	1083, 2040, 2090, 2282, 2384
TscAI	CASTG	4 cut(s)	424, 1721, 1869, 2734
TseFI	GTSAC	5 cut(s)	141, 958, 2100, 3147, 3342
TseI	GCWGC	7 cut(s)	774, 1100, 1829, 2139, 2297, 2720, 2984
Tsp45I	GTSAC	5 cut(s)	141, 958, 2100, 3147, 3342
TspDTI	ATGAA	7 cut(s)	65, 650, 1472, 1884, 2904, 2946, 3288
TspGWI	ACGGA	2 cut(s)	2364, 2646
TspRI	CASTG	4 cut(s)	424, 1721, 1869, 2734
Tth111I	GACNNNGTC	1 cut(s)	158
Van91I	CCANNNNNTGG	1 cut(s)	1558
VpaK11BI	GGWCC	5 cut(s)	154, 190, 720, 1493, 1675
XapI	RAATTY	7 cut(s)	574, 1423, 2053, 2476, 2628, 2652, 2675
XbaI	TCTAGA	3 cut(s)	1535, 1918, 3242
XceI	RCATGY	3 cut(s)	1129, 1393, 3116
XhoI	CTCGAG	1 cut(s)	1147
XmaJI	CCTAGG	1 cut(s)	2993
XmnI	GAANNNNTTC	1 cut(s)	3262
Zsp2I	ATGCAT	1 cut(s)	3024

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.