Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0004515

Physical Location & Seq

Genomic Coordinates Forward (+)

2400343 .. 2414207

Gene Structure 13865 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1016700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3402 bp

ATGTTTTATTCTCAGTTTATATTGGCCAAGAAAGGTCCTCTTGGGACAATATGGATAGCGGCACATTTGGAGCGCAAGCTTCGCAAGAATCAGGTCGCCGACACCGATATTGGCGTCTCCGTAGATTCTATTCTTTTTCCTGAAGTTCCAATTGCTCTCAGGTTGTCCAGCCATCTTCTGCTTGGTGTGGTGAGGATATATTCTAGAAAGGTGAATTACCTGTTTGATGATTGCAGTGAGGCTTTGCTTAAGATAAAGCAAGCTTTTCGCTCCACTGCAGTTGATTTACCTCCAGAAGAATCTACTGCTCCGTATCATTCCATCACCTTGCCTGAGACTTTTGATCTTGATGATTTTGAGCTACCAGATAATGAAATTTTTCAGGGTAACTATGTTGATCATCATGTCAGTACAAGGGAGCAAATAACTCTCCAAGACACAATGGAGGGTGTGGTTTACTCGACATCTCAATTTGGATTGGATGAGCGCTTTGGTGATGGTGATACTTCTCAAATTGGTCTGGAATTTGATGAGGACCTGTTCTTTGGCAAAGTTGCAGCTCAAGTGAATGATGGAATTTCAGATGGTGATCCTCAAACATTGGGCCTATCAGTAACACCTCTGGAAAAGCATGATATGTATGAGGGGATACCTGGAACTTCAGAAACCATACAAATGAATGGGATTGAAAACCAGAATGATGTCCTAGCCGCAAATACTGATTTTGTTACGTATGCTCAAGCTCCATCAACTCCTGGACTATTTGAAGAGCCAAACCTGTTTGCGGTTCAGGAGCCTATGGCCTGCAGTGATCACTTGGATTTCGAAGACCATAATTTATCAAATTTAGCATCCATGGGCAGCTCAGAAAATGCTGGTTGTATGTCAGGTCCGCGTTGTGGGGACGATAGTACAATGGTTTCTCCCAACGAGAATGGCTACCATTTGGGCGATATGGAGATCAAACAAACAAAGCCACAGGAGCATGAGCTGATTGAATCCATTTCTCCGGTATTAGAATGTTTAAATGGGACAGTTGGTGCATTGGATGGCCGAAACAGGGTTGAAGATATACACGATGGTATTGTTATAAACAATGAACCACTTATGCCTATCCTAGCTCAAAAGGATGCACAACATGCAGAACCTGCTGGAGTTAGGTTTGATGAAACTGTTGCATCTCCTAGCTACTCCCAGGTCACATCTGAGTTGGACGACCCTGCTCGTAAGATTAGTTCAGGTAATTCCTGTCCGAGAGCGTCAGAAGATTATTTGGAAGATCAGCAGACATGTTTAAAGCCTGAAATACAGATTGATGTTGAAACTGCTAATAATATGGAACAATCATGCATACCTAGCATTACAGATCCTTTCAAGCCTGTTACTCATGAAGACACGGCATCTGCTGAAGTGCCTGTGCTCCAGGCGTGTGGCTCCCTCCCAAGTCATCGTGATCTGCTATCTCCTGCCGATAAAATTTCGGAAATCCCCTTCAACTTACCATCTGAGGTTGTAGGGCCAAGTTCATTGGACAATGCAACATCTTTGGACAATCAATTGGAGAACTTGGATAGGTCTGCCACTTCTAACTTGCCTCCACCTGAAAAGTTGCTCTCTGTACCAGAGGGGTTCACTTCTAAACCAAGTGATTTTCTAGTGGAACCTACGCCTGAAAAAGAAGTCATGGGTGGGGATGCTGGTGATGGTGATGGGATTAAACTCATCTCAGGGAAAAAACGTAGTTCCACAGAAAGTACACTGACAGTGCAGAGTTTGAATTCAGTTGAATCATTTGGGGAGGCCCGAGGAAAGAGAACTGCAGAATCTATCCCTGATGATGATGATTTATTGTCTTCTATTTTAGTTGGAAGAAGGTCTTCAGTTCTAAAAATGAAACCCACTCCGCCTGCACCTGAAATGATATGCACTAAACGCTCAAGAACTGCTGCCCGCGGTACTGCCTCAAAGAGGAAAGTGCTAATGGATGATACAATGGTCTTGCATGGCGATCAAATACGCCAGCAGCTGACAAATACTGAAGACATACGTCGTGTGAGAAAAAAGGCTCCTTGTACTCGCCCAGAAATTTCAATGATTCAGCGACAATTTTTGGAAGATGCAATTTTTACTGAACCCATATTTACTGGCATGTCGGCGGAACTGATTTATCTGCACACTGGAACATTTGATACAAGTACAACAAGGGTTTCCGAAAATGAGCAAAACAATGTTTCTGCAGAACCATTGAAGGATGTAGAATCCTCTCTGGAGCCAAATGTTACTAAAGAAATTGAACCCCTTCGAAGCACTGAACCTGTAATAATGAGAGATGACTCAGCAGCACAGCCTGCTGGCGTTCCTATTGAGAACGAAAACCAGAAGGAAGAAGATCATAACCTAAGATCTCACCATACTGATGCTCAAGAACAGGGGATCACTGACCTGGAAGAATTTAATACTTCCAAACATGAACCCTTGGGGGAGATAGCTGAAATGGAAATTGACAAAGAAAATTTTGAAGGTGCTGATCCTATTGTAGAAGATATTTACAAGGTGTCAGCTGATGTTAACGTCCAGTCAAGGCTTATGGATAAAACTGATGATGAAGATGCGTTGCTACAGATGGAGGCATTATGCAGGTCACCAAATAATAAGTTGGACGCCCAACCCATGGAGGTGGATGCATTGATAGTGGAGGCAGGTATCCAGAAAGAAGTTGATGCCATTGAAGTTGTTGACCGTAATGTCGAAATCAGAGCAGATGTTTGGATAGGTGCGTCTGAACTCACCAACAATGTAAATGTTACGCTCTCAACTGGAGAATCCAAAAACCTTAGTTTTTCAGATTATCAACCTGTGGAAGGAATTGGAAATAGTGAGCAACATATTGTGAATGAAACTGAGGTCCTGGAGGCAAACTTGCATTGTGATGACAATGCTTCCTTGAATGGTAAGGAAAGTCCTGGTTGTCGGGAGGTTGATCCACAGAGTGGCATTGATGCAGAAGTCACTGCAGTTGCAGAAGATCGTGGTGATTGTGAAGATGTAGGTTTTGGAAATGATACAGAGTTTCTGAATGTAGATGATGATGAAATAACTGAAGCTGTCGATGACATGCCGTGCGCTGAAGATACTCGTCTTCTTGAGAACAGTGGATGGTCTTCCCGCACCAGGGCTGTTGCCAAGTATCTCCAGACTTTACTTGATAAGGAACCTGCGCATGGGAAAAAAGTTCTTGGCATGGACAGTCTGTTAAATGGTAAAACTCGAAAGGAAGCATCAAGGATGTTTTTTGAAACATTGGTTCTTAAGACAAGGGATTACATCCATGTGGAACAGGCAAAGCCCTTTGACAACGTCAGCATAAGATCTCGAGTAAAACTGATGAAGTCAGAGTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000278 GO:0000793 GO:0000794 GO:0000798 GO:0000819 GO:0003674 GO:0003682 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007062 GO:0007275 GO:0008150 GO:0008152 GO:0008278 GO:0009553 GO:0009555 GO:0009561 GO:0009987 GO:0016043 GO:0022402 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0048229 GO:0048856 GO:0050896 GO:0051276 GO:0051716 GO:0070013 GO:0070601 GO:0071704 GO:0071840 GO:0090304 GO:0098813 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko04110 ko04111 map04110 map04111

Pfam Domains

Protein Families

Rad21_Rec8 Rad21_Rec8_N

Protein Analysis

1134

Amino Acids

124.73

Weight (kDa)

4.44

Isoelectric Point (pI)

47.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rad21_Rec8_N	PF04825	1 - 101	7.8e-39	N terminus of Rad21 / Rec8 like protein
Rad21_Rec8	PF04824	1078 - 1125	3.9e-18	Conserved region of Rad21 / Rec8 like protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0004515)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G16270
fragaria_vesca	FvH4_6g00010 FvH4_6g00010 FvH4_6g00010 FvH4_6g00010 FvH4_6g00010 FvH4_6g00010 FvH4_6g00010 FvH4_6g00010 FvH4_6g00010 FvH4_6g00010
malus_domestica	MD00G1016700.v1.1
prunus_persica	Prupe.6G366000_v2.0.a1 Prupe.6G366000_v2.0.a1
pyrus_communis	pycom04g22040 pycom10g04620 pycom12g24300
rosa_chinensis	RchiOBHm_Chr3g0447331
rosa_laevigata	RLG00000026010
rosa_multiflora	Rmu_ssc0000473.1_g000001
rosa_roxburghii	Rroxscaffold_6G00425510
rosa_rugosa	Rorug02G0599600.1 Rorug02G0599700.1 Rorug02G0599800.1 Rorug02G0599900
rosa_samantha	Rh3AG000300 Rh3BG000100 Rh3CG000100 Rh3DG000300 Rh5AG542800 Rh5AG542900
rosa_wichuraiana	Rw3G000010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1091
AasI	GACNNNNNNGTC	1 cut(s)	2744
Acc16I	TGCGCA	1 cut(s)	3219
Acc36I	ACCTGC	4 cut(s)	1156, 2628, 2690, 3223
AccB7I	CCANNNNNTGG	2 cut(s)	2671, 2990
AccII	CGCG	2 cut(s)	895, 1953
AciI	CCGC	9 cut(s)	59, 711, 785, 893, 1904, 1951, 1953, 2156, 3166
AclWI	GGATC	5 cut(s)	584, 1361, 2441, 2522, 2975
AcoI	YGGCCR	2 cut(s)	24, 1051
AcsI	RAATTY	9 cut(s)	375, 524, 576, 844, 1476, 1777, 2085, 2450, 2512
AcuI	CTGAAG	7 cut(s)	162, 645, 1428, 1863, 2058, 3120, 3147
AcyI	GRCGYC	2 cut(s)	114, 2661
AdeI	CACNNNGTG	1 cut(s)	2990
AfaI	GTAC	7 cut(s)	412, 913, 1620, 1756, 1957, 2074, 2197
AfeI	AGCGCT	1 cut(s)	488
AflII	CTTAAG	2 cut(s)	248, 3308
AflIII	ACRYGT	1 cut(s)	1289
AjnI	CCWGG	8 cut(s)	652, 754, 1194, 1422, 2442, 2907, 2962, 3170
Alw21I	GWGCWC	1 cut(s)	1422
Alw26I	GTCTC	2 cut(s)	121, 329
AlwI	GGATC	5 cut(s)	584, 1361, 2441, 2522, 2975
AlwNI	CAGNNNCTG	2 cut(s)	1148, 2026
Ama87I	CYCGRG	2 cut(s)	1803, 3372
Aor51HI	AGCGCT	1 cut(s)	488
AoxI	GGCC	6 cut(s)	24, 604, 801, 1051, 1517, 1800
ApeKI	GCWGC	5 cut(s)	557, 861, 1946, 2023, 2339
ApoI	RAATTY	9 cut(s)	375, 524, 576, 844, 1476, 1777, 2085, 2450, 2512
ArsI	GACNNNNNNTTYG	2 cut(s)	527, 559
Asp700I	GAANNNNTTC	3 cut(s)	378, 1876, 2298
AspLEI	GCGC	4 cut(s)	75, 489, 3125, 3220
AspS9I	GGNCC	7 cut(s)	35, 535, 604, 890, 1517, 1801, 2905
AsuII	TTCGAA	2 cut(s)	825, 2302
AvaI	CYCGRG	2 cut(s)	1803, 3372
AvaII	GGWCC	4 cut(s)	35, 535, 890, 2905
BaeI	ACNNNNGTAYC	2 cut(s)	1980, 2013
BalI	TGGCCA	1 cut(s)	26
BbsI	GAAGAC	7 cut(s)	834, 1398, 1845, 1869, 2046, 3131, 3153
Bbv12I	GWGCWC	1 cut(s)	1422
BbvI	GCAGC	5 cut(s)	569, 873, 1933, 2035, 2351
BceAI	ACGGC	2 cut(s)	1413, 3103
BcgI	CGANNNNNNTGC	2 cut(s)	248, 282
BciT130I	CCWGG	8 cut(s)	654, 756, 1196, 1424, 2444, 2909, 2964, 3172
BciVI	GTATCC	2 cut(s)	642, 2714
BclI	TGATCA	2 cut(s)	397, 811
BcoDI	GTCTC	2 cut(s)	121, 329
BfaI	CTAG	6 cut(s)	204, 707, 1118, 1185, 1356, 1655
BfmI	CTRYAG	6 cut(s)	276, 805, 1818, 2235, 2618, 3012
BfoI	RGCGCY	1 cut(s)	490
BfrI	CTTAAG	2 cut(s)	248, 3308
BfuAI	ACCTGC	4 cut(s)	1156, 2628, 2690, 3223
BfuI	GTATCC	2 cut(s)	642, 2714
BglII	AGATCT	2 cut(s)	2402, 3368
BisI	GCNGC	7 cut(s)	60, 558, 711, 862, 1947, 2024, 2340
BlsI	GCNGC	7 cut(s)	61, 559, 712, 863, 1948, 2025, 2341
Bme1390I	CCNGG	8 cut(s)	654, 756, 1196, 1424, 2444, 2909, 2964, 3172
Bme18I	GGWCC	4 cut(s)	35, 535, 890, 2905
BmeT110I	CYCGRG	2 cut(s)	1803, 3372
BmgT120I	GGNCC	7 cut(s)	35, 535, 604, 890, 1517, 1801, 2905
BmiI	GGNNCC	6 cut(s)	795, 1435, 1662, 2067, 2271, 3213
BmrFI	CCNGG	8 cut(s)	654, 756, 1196, 1424, 2444, 2909, 2964, 3172
BoxI	GACNNNNGTC	1 cut(s)	2046
BpiI	GAAGAC	7 cut(s)	834, 1398, 1845, 1869, 2046, 3131, 3153
BpmI	CTGGAG	7 cut(s)	276, 1173, 1406, 2288, 2838, 2930, 3176
Bpu14I	TTCGAA	2 cut(s)	825, 2302
BpuEI	CTTGAG	5 cut(s)	546, 723, 1921, 2406, 3164
BsaAI	YACGTR	1 cut(s)	732
BsaBI	GATNNNNATC	1 cut(s)	588
BsaHI	GRCGYC	2 cut(s)	114, 2661
BsaJI	CCNNGG	7 cut(s)	855, 1194, 1804, 1951, 2475, 2670, 3171
BsaWI	WCCGGW	1 cut(s)	1009
Bse1I	ACTGG	4 cut(s)	2149, 2182, 2575, 2821
Bse8I	GATNNNNATC	1 cut(s)	588
BseBI	CCWGG	8 cut(s)	654, 756, 1196, 1424, 2444, 2909, 2964, 3172
BseDI	CCNNGG	7 cut(s)	855, 1194, 1804, 1951, 2475, 2670, 3171
BseJI	GATNNNNATC	1 cut(s)	588
BseMII	CTCAG	9 cut(s)	26, 172, 324, 879, 1197, 1497, 1740, 2349, 2892
BseNI	ACTGG	4 cut(s)	2149, 2182, 2575, 2821
BseXI	GCAGC	5 cut(s)	569, 873, 1933, 2035, 2351
BsgI	GTGCAG	3 cut(s)	1787, 1893, 2156
Bsh1236I	CGCG	2 cut(s)	895, 1953
BshFI	GGCC	6 cut(s)	26, 606, 803, 1053, 1519, 1802
BsiHKAI	GWGCWC	1 cut(s)	1422
BsiHKCI	CYCGRG	2 cut(s)	1803, 3372
BsiSI	CCGG	1 cut(s)	1010
BslFI	GGGAC	3 cut(s)	58, 917, 1045
BsmAI	GTCTC	2 cut(s)	121, 329
BsmBI	CGTCTC	1 cut(s)	121
BsmFI	GGGAC	3 cut(s)	58, 917, 1045
BsnI	GGCC	6 cut(s)	26, 606, 803, 1053, 1519, 1802
BsoBI	CYCGRG	2 cut(s)	1803, 3372
Bsp119I	TTCGAA	2 cut(s)	825, 2302
Bsp1286I	GDGCHC	1 cut(s)	1422
Bsp19I	CCATGG	2 cut(s)	855, 2670
BspACI	CCGC	9 cut(s)	59, 711, 785, 893, 1904, 1951, 1953, 2156, 3166
BspANI	GGCC	6 cut(s)	26, 606, 803, 1053, 1519, 1802
BspCNI	CTCAG	9 cut(s)	25, 171, 325, 878, 1198, 1498, 1739, 2348, 2893
BspFNI	CGCG	2 cut(s)	895, 1953
BspHI	TCATGA	1 cut(s)	1387
BspLI	GGNNCC	6 cut(s)	795, 1435, 1662, 2067, 2271, 3213
BspMAI	CTGCAG	5 cut(s)	280, 809, 1822, 2239, 3016
BspMI	ACCTGC	4 cut(s)	1156, 2628, 2690, 3223
BspPI	GGATC	5 cut(s)	584, 1361, 2441, 2522, 2975
BspQI	GCTCTTC	1 cut(s)	762
BspT104I	TTCGAA	2 cut(s)	825, 2302
BspTI	CTTAAG	2 cut(s)	248, 3308
BsrI	ACTGG	4 cut(s)	2149, 2182, 2575, 2821
BssECI	CCNNGG	7 cut(s)	855, 1194, 1804, 1951, 2475, 2670, 3171
BssNI	GRCGYC	2 cut(s)	114, 2661
BssT1I	CCWWGG	3 cut(s)	855, 2475, 2670
Bst2UI	CCWGG	8 cut(s)	654, 756, 1196, 1424, 2444, 2909, 2964, 3172
Bst4CI	ACNGT	6 cut(s)	1036, 1174, 1765, 2741, 3152, 3248
Bst6I	CTCTTC	1 cut(s)	762
BstACI	GRCGYC	2 cut(s)	114, 2661
BstAFI	CTTAAG	2 cut(s)	248, 3308
BstAPI	GCANNNNNTGC	3 cut(s)	1139, 1148, 2348
BstBAI	YACGTR	1 cut(s)	732
BstBI	TTCGAA	2 cut(s)	825, 2302
BstC8I	GCNNGC	8 cut(s)	77, 261, 805, 1908, 1951, 2021, 2349, 2353
BstDSI	CCRYGG	3 cut(s)	855, 1951, 2670
BstEII	GGTNACC	1 cut(s)	2640
BstENI	CCTNNNNNAGG	2 cut(s)	1966, 2859
BstFNI	CGCG	2 cut(s)	895, 1953
BstH2I	RGCGCY	1 cut(s)	490
BstHHI	GCGC	4 cut(s)	75, 489, 3125, 3220
BstMAI	GTCTC	2 cut(s)	121, 329
BstMWI	GCNNNNNNNGC	7 cut(s)	81, 982, 1139, 1148, 1356, 1932, 2348
BstNI	CCWGG	8 cut(s)	654, 756, 1196, 1424, 2444, 2909, 2964, 3172
BstNSI	RCATGY	4 cut(s)	1142, 1293, 2152, 3118
BstPAI	GACNNNNGTC	1 cut(s)	2046
BstPI	GGTNACC	1 cut(s)	2640
BstSCI	CCNGG	8 cut(s)	652, 754, 1194, 1422, 2442, 2907, 2962, 3170
BstSFI	CTRYAG	6 cut(s)	276, 805, 1818, 2235, 2618, 3012
BstSNI	TACGTA	1 cut(s)	732
BstUI	CGCG	2 cut(s)	895, 1953
BstV1I	GCAGC	5 cut(s)	569, 873, 1933, 2035, 2351
BstV2I	GAAGAC	7 cut(s)	834, 1398, 1845, 1869, 2046, 3131, 3153
BstX2I	RGATCY	3 cut(s)	1366, 2402, 3368
BstXI	CCANNNNNNTGG	2 cut(s)	1430, 2677
BstYI	RGATCY	3 cut(s)	1366, 2402, 3368
BsuI	GTATCC	2 cut(s)	642, 2714
BsuRI	GGCC	6 cut(s)	26, 606, 803, 1053, 1519, 1802
BtgI	CCRYGG	3 cut(s)	855, 1951, 2670
BtsI	GCAGTG	4 cut(s)	241, 273, 814, 3009
BveI	ACCTGC	4 cut(s)	1156, 2628, 2690, 3223
Cac8I	GCNNGC	8 cut(s)	77, 261, 805, 1908, 1951, 2021, 2349, 2353
CaiI	CAGNNNCTG	2 cut(s)	1148, 2026
CciI	TCATGA	1 cut(s)	1387
CfoI	GCGC	4 cut(s)	75, 489, 3125, 3220
Cfr13I	GGNCC	7 cut(s)	35, 535, 604, 890, 1517, 1801, 2905
Cfr42I	CCGCGG	1 cut(s)	1954
CseI	GACGC	4 cut(s)	103, 1248, 2669, 2766
Csp6I	GTAC	7 cut(s)	411, 912, 1619, 1755, 1956, 2073, 2196
CspCI	CAANNNNNGTGG	2 cut(s)	262, 297
CviQI	GTAC	7 cut(s)	411, 912, 1619, 1755, 1956, 2073, 2196
DraI	TTTAAA	2 cut(s)	1026, 1296
DraIII	CACNNNGTG	1 cut(s)	2990
DrdI	GACNNNNNNGTC	1 cut(s)	2744
DseDI	GACNNNNNNGTC	1 cut(s)	2744
EaeI	YGGCCR	2 cut(s)	24, 1051
Eam1104I	CTCTTC	1 cut(s)	762
EarI	CTCTTC	1 cut(s)	762
EciI	GGCGGA	2 cut(s)	1893, 2171
Eco105I	TACGTA	1 cut(s)	732
Eco130I	CCWWGG	3 cut(s)	855, 2475, 2670
Eco47I	GGWCC	4 cut(s)	35, 535, 890, 2905
Eco47III	AGCGCT	1 cut(s)	488
Eco57I	CTGAAG	7 cut(s)	162, 645, 1428, 1863, 2058, 3120, 3147
Eco88I	CYCGRG	2 cut(s)	1803, 3372
Eco91I	GGTNACC	1 cut(s)	2640
EcoNI	CCTNNNNNAGG	2 cut(s)	1966, 2859
EcoO109I	RGGNCCY	3 cut(s)	35, 535, 2905
EcoO65I	GGTNACC	1 cut(s)	2640
EcoRI	GAATTC	1 cut(s)	1777
EcoRII	CCWGG	8 cut(s)	652, 754, 1194, 1422, 2442, 2907, 2962, 3170
EcoT14I	CCWWGG	3 cut(s)	855, 2475, 2670
EcoT22I	ATGCAT	2 cut(s)	1352, 2686
ErhI	CCWWGG	3 cut(s)	855, 2475, 2670
Esp3I	CGTCTC	1 cut(s)	121
FalI	AAGNNNNNCTT	4 cut(s)	24, 56, 1861, 1893
FaqI	GGGAC	3 cut(s)	58, 917, 1045
FauI	CCCGC	2 cut(s)	1958, 3173
FbaI	TGATCA	2 cut(s)	397, 811
Fnu4HI	GCNGC	7 cut(s)	60, 558, 711, 862, 1947, 2024, 2340
Fsp4HI	GCNGC	7 cut(s)	60, 558, 711, 862, 1947, 2024, 2340
FspBI	CTAG	6 cut(s)	204, 707, 1118, 1185, 1356, 1655
FspI	TGCGCA	1 cut(s)	3219
GlaI	GCGC	4 cut(s)	74, 488, 3124, 3219
GluI	GCNGC	7 cut(s)	60, 558, 711, 862, 1947, 2024, 2340
GsuI	CTGGAG	7 cut(s)	276, 1173, 1406, 2288, 2838, 2930, 3176
HaeII	RGCGCY	1 cut(s)	490
HaeIII	GGCC	6 cut(s)	26, 606, 803, 1053, 1519, 1802
HapII	CCGG	1 cut(s)	1010
HgaI	GACGC	4 cut(s)	103, 1248, 2669, 2766
HhaI	GCGC	4 cut(s)	75, 489, 3125, 3220
Hin1I	GRCGYC	2 cut(s)	114, 2661
Hin6I	GCGC	4 cut(s)	73, 487, 3123, 3218
HinP1I	GCGC	4 cut(s)	73, 487, 3123, 3218
HincII	GTYRAC	2 cut(s)	2569, 2737
HindII	GTYRAC	2 cut(s)	2569, 2737
HindIII	AAGCTT	2 cut(s)	77, 261
HpaI	GTTAAC	1 cut(s)	2569
HpaII	CCGG	1 cut(s)	1010
Hpy166II	GTNNAC	5 cut(s)	457, 1632, 1757, 2569, 2737
Hpy8I	GTNNAC	5 cut(s)	457, 1632, 1757, 2569, 2737
Hpy99I	CGWCG	1 cut(s)	2052
HpyAV	CCTTC	7 cut(s)	1501, 1866, 2242, 2309, 2374, 2513, 2855
HpyCH4III	ACNGT	6 cut(s)	1036, 1174, 1765, 2741, 3152, 3248
HpyCH4IV	ACGT	5 cut(s)	731, 1738, 2047, 2571, 3357
HpyF10VI	GCNNNNNNNGC	7 cut(s)	81, 982, 1139, 1148, 1356, 1932, 2348
HpySE526I	ACGT	5 cut(s)	731, 1738, 2047, 2571, 3357
Hsp92I	GRCGYC	2 cut(s)	114, 2661
HspAI	GCGC	4 cut(s)	73, 487, 3123, 3218
Ksp22I	TGATCA	2 cut(s)	397, 811
KspAI	GTTAAC	1 cut(s)	2569
KspI	CCGCGG	1 cut(s)	1954
LguI	GCTCTTC	1 cut(s)	762
Lsp1109I	GCAGC	5 cut(s)	569, 873, 1933, 2035, 2351
MaeI	CTAG	6 cut(s)	204, 707, 1118, 1185, 1356, 1655
MaeII	ACGT	5 cut(s)	731, 1738, 2047, 2571, 3357
MaeIII	GTNAC	9 cut(s)	386, 613, 727, 1198, 1381, 2278, 2640, 2803, 3007
MfeI	CAATTG	2 cut(s)	150, 1556
MflI	RGATCY	3 cut(s)	1366, 2402, 3368
MhlI	GDGCHC	1 cut(s)	1422
MlsI	TGGCCA	1 cut(s)	26
MluNI	TGGCCA	1 cut(s)	26
MlyI	GAGTC	1 cut(s)	2327
MmeI	TCCRAC	3 cut(s)	1191, 1846, 2637
Mox20I	TGGCCA	1 cut(s)	26
Mph1103I	ATGCAT	2 cut(s)	1352, 2686
MroXI	GAANNNNTTC	3 cut(s)	378, 1876, 2298
MscI	TGGCCA	1 cut(s)	26
MseI	TTAA	8 cut(s)	249, 1025, 1295, 1716, 2454, 2568, 3254, 3309
MslI	CAYNNNNRTG	5 cut(s)	674, 2415, 2675, 2928, 3329
Msp20I	TGGCCA	1 cut(s)	26
MspA1I	CMGCKG	3 cut(s)	1953, 2026, 2561
MspCI	CTTAAG	2 cut(s)	248, 3308
MspI	CCGG	1 cut(s)	1010
MspR9I	CCNGG	8 cut(s)	654, 756, 1196, 1424, 2444, 2909, 2964, 3172
MunI	CAATTG	2 cut(s)	150, 1556
MvaI	CCWGG	8 cut(s)	654, 756, 1196, 1424, 2444, 2909, 2964, 3172
MvnI	CGCG	2 cut(s)	895, 1953
MwoI	GCNNNNNNNGC	7 cut(s)	81, 982, 1139, 1148, 1356, 1932, 2348
NcoI	CCATGG	2 cut(s)	855, 2670
NlaIV	GGNNCC	6 cut(s)	795, 1435, 1662, 2067, 2271, 3213
NmuCI	GTSAC	3 cut(s)	1198, 2640, 3007
NsbI	TGCGCA	1 cut(s)	3219
NsiI	ATGCAT	2 cut(s)	1352, 2686
NspI	RCATGY	4 cut(s)	1142, 1293, 2152, 3118
NspV	TTCGAA	2 cut(s)	825, 2302
PaeR7I	CTCGAG	1 cut(s)	3372
PagI	TCATGA	1 cut(s)	1387
PciI	ACATGT	1 cut(s)	1289
PciSI	GCTCTTC	1 cut(s)	762
PcsI	WCGNNNNNNNCGW	1 cut(s)	102
PdmI	GAANNNNTTC	3 cut(s)	378, 1876, 2298
PfeI	GAWTC	9 cut(s)	88, 125, 299, 998, 1787, 1823, 2095, 2258, 2822
PflFI	GACNNNGTC	1 cut(s)	3356
PflMI	CCANNNNNTGG	2 cut(s)	2671, 2990
PfoI	TCCNGGA	2 cut(s)	754, 2907
PkrI	GCNGC	7 cut(s)	61, 559, 712, 863, 1948, 2025, 2341
PleI	GAGTC	1 cut(s)	2327
PpsI	GAGTC	1 cut(s)	2327
Ppu21I	YACGTR	1 cut(s)	732
PpuMI	RGGWCCY	3 cut(s)	35, 535, 2905
PscI	ACATGT	1 cut(s)	1289
PshAI	GACNNNNGTC	1 cut(s)	2046
PsiI	TTATAA	1 cut(s)	1091
Psp5II	RGGWCCY	3 cut(s)	35, 535, 2905
Psp6I	CCWGG	8 cut(s)	652, 754, 1194, 1422, 2442, 2907, 2962, 3170
PspEI	GGTNACC	1 cut(s)	2640
PspGI	CCWGG	8 cut(s)	652, 754, 1194, 1422, 2442, 2907, 2962, 3170
PspN4I	GGNNCC	6 cut(s)	795, 1435, 1662, 2067, 2271, 3213
PspPI	GGNCC	7 cut(s)	35, 535, 604, 890, 1517, 1801, 2905
PspPPI	RGGWCCY	3 cut(s)	35, 535, 2905
PsrI	GAACNNNNNNTAC	4 cut(s)	1219, 1251, 2173, 2205
PstI	CTGCAG	5 cut(s)	280, 809, 1822, 2239, 3016
PstNI	CAGNNNCTG	2 cut(s)	1148, 2026
PsuI	RGATCY	3 cut(s)	1366, 2402, 3368
PsyI	GACNNNGTC	1 cut(s)	3356
PvuII	CAGCTG	2 cut(s)	2026, 2561
RsaI	GTAC	7 cut(s)	412, 913, 1620, 1756, 1957, 2074, 2197
RsaNI	GTAC	7 cut(s)	411, 912, 1619, 1755, 1956, 2073, 2196
RseI	CAYNNNNRTG	5 cut(s)	674, 2415, 2675, 2928, 3329
SacII	CCGCGG	1 cut(s)	1954
SapI	GCTCTTC	1 cut(s)	762
SaqAI	TTAA	8 cut(s)	249, 1025, 1295, 1716, 2454, 2568, 3254, 3309
SatI	GCNGC	7 cut(s)	60, 558, 711, 862, 1947, 2024, 2340
Sau96I	GGNCC	7 cut(s)	35, 535, 604, 890, 1517, 1801, 2905
SchI	GAGTC	1 cut(s)	2327
ScrFI	CCNGG	8 cut(s)	654, 756, 1196, 1424, 2444, 2909, 2964, 3172
SduI	GDGCHC	1 cut(s)	1422
SfcI	CTRYAG	6 cut(s)	276, 805, 1818, 2235, 2618, 3012
Sfr274I	CTCGAG	1 cut(s)	3372
Sfr303I	CCGCGG	1 cut(s)	1954
SfuI	TTCGAA	2 cut(s)	825, 2302
SgrBI	CCGCGG	1 cut(s)	1954
SinI	GGWCC	4 cut(s)	35, 535, 890, 2905
SlaI	CTCGAG	1 cut(s)	3372
SmiMI	CAYNNNNRTG	5 cut(s)	674, 2415, 2675, 2928, 3329
SmlI	CTYRAG	8 cut(s)	248, 561, 738, 1936, 2421, 3143, 3308, 3372
SmoI	CTYRAG	8 cut(s)	248, 561, 738, 1936, 2421, 3143, 3308, 3372
SnaBI	TACGTA	1 cut(s)	732
SsiI	CCGC	9 cut(s)	59, 711, 785, 893, 1904, 1951, 1953, 2156, 3166
SspMI	CTAG	6 cut(s)	204, 707, 1118, 1185, 1356, 1655
StyD4I	CCNGG	8 cut(s)	652, 754, 1194, 1422, 2442, 2907, 2962, 3170
StyI	CCWWGG	3 cut(s)	855, 2475, 2670
TaaI	ACNGT	6 cut(s)	1036, 1174, 1765, 2741, 3152, 3248
TaiI	ACGT	5 cut(s)	734, 1741, 2050, 2574, 3360
TaqI	TCGA	7 cut(s)	461, 825, 2302, 2748, 3108, 3268, 3373
TatI	WGTACW	5 cut(s)	410, 911, 1754, 2072, 2195
TauI	GCSGC	2 cut(s)	62, 713
TfiI	GAWTC	9 cut(s)	88, 125, 299, 998, 1787, 1823, 2095, 2258, 2822
Tru1I	TTAA	8 cut(s)	249, 1025, 1295, 1716, 2454, 2568, 3254, 3309
Tru9I	TTAA	8 cut(s)	249, 1025, 1295, 1716, 2454, 2568, 3254, 3309
TseFI	GTSAC	3 cut(s)	1198, 2640, 3007
TseI	GCWGC	5 cut(s)	557, 861, 1946, 2023, 2339
Tsp45I	GTSAC	3 cut(s)	1198, 2640, 3007
TspGWI	ACGGA	2 cut(s)	109, 300
Tth111I	GACNNNGTC	1 cut(s)	3356
Van91I	CCANNNNNTGG	2 cut(s)	2671, 2990
Vha464I	CTTAAG	2 cut(s)	248, 3308
VpaK11BI	GGWCC	4 cut(s)	35, 535, 890, 2905
XagI	CCTNNNNNAGG	2 cut(s)	1966, 2859
XapI	RAATTY	9 cut(s)	375, 524, 576, 844, 1476, 1777, 2085, 2450, 2512
XbaI	TCTAGA	1 cut(s)	203
XceI	RCATGY	4 cut(s)	1142, 1293, 2152, 3118
XcmI	CCANNNNNNNNNTGG	1 cut(s)	179
XhoI	CTCGAG	1 cut(s)	3372
XmnI	GAANNNNTTC	3 cut(s)	378, 1876, 2298
XspI	CTAG	6 cut(s)	204, 707, 1118, 1185, 1356, 1655
Zsp2I	ATGCAT	2 cut(s)	1352, 2686

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.