Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3B

Orthogroup ID

OG0004515

Physical Location & Seq

Genomic Coordinates Reverse (-)

43992 .. 53729

Gene Structure 9738 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3BG000100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3480 bp

ATGTTTTACTCGCAGTTTATATTGGCAAAGAAAGGCCCACTTGGGACGATATGGATAGCGGCACATTTGGAGCGCAAGCTTCGCAAGAATCAGGTTGCCGACACCGATATTGGCGTCTCTGTAGATTCTATTCTCTTTCCAGAAGTTCCGATTGCTCTTAGGTTGTCCAGTCATCTTCTGCTTGGTGTAGTGAGGATATATTCTAGAAAGGTGAATTACCTTTTTGATGACTGCAGTGAGGCTTTGCTTAAGATAAAGCAAGCTTTTCGCTCCACCGCAGTCGATTTACCTCCAGAAGAATCTACTGCACCGTATCATTCTATCACTTTGCCTGAGACTTTTGATCTTGATGATTTTGAGCTACCAGATAACGACATTTTTCAGGGTAACTATGTTGATCATCATGTCAGTACAAGGGAACAGATTACTCTCCAAGATGCAATGGAGGGTGTGGTTTACTCAACATCTCAGTTTGGATTGGACGAGCGATTTGGTGATGGTGATACTTCTCAAATTGGTCTAGATTTTGATGAGGACTTGTTCTTAGGCAAGGCTGCAGCTCAAGGGAATGATGGAATTTCCGGTGGCGATCCCGAGGCATTGGCCCAGCCAACAACACCCCTGGAAAAGCATGAAGTTTTTGAGGGAGTACCTGGAACTTCAGAAACTGTGCAAATGAATGGGACTGGAAACCAGAATGAAGTCCTAGCTGCAAATACTGAATTTGTCACATATGCTCAGGCTCCATCAACTCCTGGATTATTTGAAGAGCCAAACTTGTCCAGCGTTCAGGAAGCTATGGCCTGCAATGATCACTTGGATTTGGAAGACCATAGTTTATCAAATTTACGAGTACCGGAAAGCACAGAAAATGCTTGTAGCGAGTTAGGTCCACGTTGTGAGGATGATAATACAATGAATTTGCCTGAGAATGGATACAATTTGGGAGATGTGGAGATGAAACAAATGATTCCACCAGTGCATGAGCAGATTAAACCTGTTTCTCCAGTTTTAGAATGTTCAAATGGGACGGTTGGTGCGCTGGATTTCCCAAACAGGTTGGAAGATATAAATAATGGTATTGTAATAAACAGTGAACCAACTATGCTCTTCATAGAAAAAAGGGATGAACAATGTGTAGAACCTGCAGGAGTTAGGTTGGATGAAACCGTTGCATCTCCCAGTTGCTCTCAGGTTACCTCCGAGTTGGAGGAGTCTGCTCGTAAGGTTTCTTCAAGTGGTACCTGTGTGCGAGTGCCGGAGGATTATTTGGAGGATCAACAGACATCTCTGAAGTCTGAAATTCAAAATGATATTGCTATTGCTAATGATATGGGAGAAGCATGCACACCTAACATTGTAGATTCTTCCAACCCTGTAGCTCATGAAAAGATGGCATCTGCCCAACACTCTGTGCTCCAGGCATGCAACTCCCATCTGAGTCATCACGGTGTGCTATCTCCTTCAGATAAAAGTGCAGAAATTCCTTGCAACTTATCATCTGAGGTTGTGCGGTTGAAGTCAGTGGACAATGCTTGCGACAATCAACCAGATGTTTTGGATAGGTCTGCTACTTCTGACTTGCTTGCACCTGAAAAGTTTCTATCTATACCGGACGGGCTCACTGCTGAACCAAGTGATTTACCAATGGAGTCTACACCTGACAAAGAAGTCTTTGGTGGGGATGGTGTAGATGGTGCTGGGACCAAACTCATCTCAGGGAAGAAACGCAGTTCTACAGAAAGTACTGTAACAGTTCAAAGTTTAAATTCAGTTGAGTCATTTGGGGAGGCCCGAGGAAAGCGAACTGCAGATTCCATCCCTGATGATGATGATTTATTGTCTTCTATTTTAGTTGGGAGAAAATCTTCAGTTTTAAAACTGAAACCGACTCCGCCTGTACCTGAAATGAAAACCGCAAAACGCTCCCGAACTGCTGTTCGTGGCACTGCCTCAAAGAGAAAAGTGCTAATGGATGATACAATGGTTCTGCATGGCGATACAATACGCCAGCAGTTGACAAATACTGAAGATATACGTCGTGTGAGGAAAAAAGCCCCTTGTACTCGCCCAGAAATTTCAATGATTCAGAGACAAAATTTGGAAGATGAAATTTTCATTGAACCCATTGTCACAGGCATGTCGGGGGAACTGACATTTCTGCACACTGAGAATTTTGATCTGAGTAGAACCAGGTTTTCTGAAGATGATCAAGACAATGCTTCTGACGAGGTACTGAAGGATGTACGGTATTCCGTGAGGTCAAATGTTACTGAAGAATCTGAAATCCTTGGAAGCACTGAGCCTGTAATAGTAAGAGATGATGCAGAAGCACAAGCTGACATTGTTATTGAGACTAAAAACTGGGGGATGCAAGATCATAACCTAAGATCTCAGGATACTGATGCTCAAGGGCAGGGGATTACCAACCCGGAAGAATCGAAGCATGAACCCTTGGCGGAGGTGAGTGAAATGGAAATTGATGTAAACAATGCAGAAGTTACCTATCCTGTTTCAGGAGATATATACAATATGCCATCTGAAGATAACATCCAGCCAAGGCTCCTGAATAAAATTGATGAGGAAGATGCTTCTCTGCAGATGGGTGCATTATGCATGTCCCCAGATGAAAAGTTGGACAGTCGACCTATTGAGGTGGATGCGTTGATAGTGGATGCAAGTATTCAGAAAGGAGTTGACGCTATTGGATTTGTTGAGCATAATGTCGAAATCAGAGCAGATGTTCAGATGGGTTTTTCTGAACTTACTGACATAAATTCTACACTTGCAACCGTATCTCTTGAAACTGGCGACAACAAAAACCTGAGTTCTTCAAATGATCAATCTATGGAAGAAATGGGACATGAGCTGCATATTTTGAATGAAAATGAGGTCCTGGATGCAAGCTTTGGTTGTGATGACAAGGACACCAAGTCTAGTTGTATGCTTGGTGGAGAAGACAACATTGGTTCTACAATTTCGTTGGAACTTGATGTGGATGCAAAGTATATTTCCTTCAGTGATAAGGAAAACCTGGAACATGAGGAGGCTGATCCACGGAGTGGAACTGAGGCAGAAGTCACTGCAGACCACCCTGCTGCAAATTGTGGAGATTGTGGCGATCTAGTTTTTGGAAATGATACAGAATTTTTGAATGTAGATGATGAAGAGATAGCCGAGGAAGGGGATGACATGCCGACTGCTGAAGACACTAGTCTTCTTGAAAACAGTGGATGGTCTTCTCGCACCAGGGCTGTTGCCAAGTATCTTCAAACTTTATTTGATAAGGAAGCTGTGCATGGGAAAAAAGTTCTTGGCATGGACAACTTATTAAATGGAAAAACTCGCAAGGAAGCTTCAAGGATGTTTTTTGAAACATTGGTTCTTAAGACAAGGGATTACATCAACGTAGACCAGGCAAAACCCTTTGACAACATTAACATAAAGCCTAGAGTAAAGCTCATGAAATCAGACTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000278 GO:0000793 GO:0000794 GO:0000798 GO:0000819 GO:0003674 GO:0003682 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007062 GO:0007275 GO:0008150 GO:0008152 GO:0008278 GO:0009553 GO:0009555 GO:0009561 GO:0009987 GO:0016043 GO:0022402 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0048229 GO:0048856 GO:0050896 GO:0051276 GO:0051716 GO:0070013 GO:0070601 GO:0071704 GO:0071840 GO:0090304 GO:0098813 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko04110 ko04111 map04110 map04111

Pfam Domains

Protein Families

Rad21_Rec8 Rad21_Rec8_N

Protein Analysis

1159

Amino Acids

127.12

Weight (kDa)

4.4

Isoelectric Point (pI)

46.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rad21_Rec8_N	PF04825	1 - 101	8e-39	N terminus of Rad21 / Rec8 like protein
Rad21_Rec8	PF04824	1104 - 1153	1.4e-18	Conserved region of Rad21 / Rec8 like protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004515)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G16270
fragaria_vesca	FvH4_6g00010 FvH4_6g00010 FvH4_6g00010 FvH4_6g00010 FvH4_6g00010 FvH4_6g00010 FvH4_6g00010 FvH4_6g00010 FvH4_6g00010 FvH4_6g00010
malus_domestica	MD00G1016700.v1.1
prunus_persica	Prupe.6G366000_v2.0.a1 Prupe.6G366000_v2.0.a1
pyrus_communis	pycom04g22040 pycom10g04620 pycom12g24300
rosa_chinensis	RchiOBHm_Chr3g0447331
rosa_laevigata	RLG00000026010
rosa_multiflora	Rmu_ssc0000473.1_g000001
rosa_roxburghii	Rroxscaffold_6G00425510
rosa_rugosa	Rorug02G0599600.1 Rorug02G0599700.1 Rorug02G0599800.1 Rorug02G0599900
rosa_samantha	Rh3AG000300 Rh3BG000100 Rh3CG000100 Rh3DG000300 Rh5AG542800 Rh5AG542900
rosa_wichuraiana	Rw3G000010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1670
Acc36I	ACCTGC	1 cut(s)	1153
Acc65I	GGTACC	1 cut(s)	1241
AccB1I	GGYRCC	1 cut(s)	1241
AccB7I	CCANNNNNTGG	1 cut(s)	3062
AccI	GTMKAC	3 cut(s)	1655, 2644, 3411
AciI	CCGC	6 cut(s)	59, 276, 1513, 1895, 1917, 2459
AclWI	GGATC	3 cut(s)	584, 1284, 3047
AcyI	GRCGYC	1 cut(s)	114
AdeI	CACNNNGTG	4 cut(s)	899, 1414, 1453, 3062
AfaI	GTAC	9 cut(s)	412, 651, 855, 1243, 1747, 1902, 2065, 2235, 2247
AfiI	CCNNNNNNNGG	4 cut(s)	932, 2516, 3062, 3183
AflII	CTTAAG	2 cut(s)	248, 3386
AhdI	GACNNNNNGTC	1 cut(s)	2932
AhlI	ACTAGT	1 cut(s)	3212
AjnI	CCWGG	9 cut(s)	621, 652, 754, 1419, 2192, 2895, 3033, 3248, 3414
AjuI	GAANNNNNNNTTGG	2 cut(s)	2949, 2981
Alw21I	GWGCWC	1 cut(s)	1419
Alw26I	GTCTC	4 cut(s)	121, 329, 2086, 2348
AlwI	GGATC	3 cut(s)	584, 1284, 3047
AlwNI	CAGNNNCTG	1 cut(s)	668
Ama87I	CYCGRG	2 cut(s)	593, 1794
AoxI	GGCC	4 cut(s)	34, 603, 801, 1791
ApeKI	GCWGC	5 cut(s)	554, 557, 710, 2869, 3098
ArsI	GACNNNNNNTTYG	2 cut(s)	473, 505
Asp700I	GAANNNNTTC	2 cut(s)	1599, 1867
Asp718I	GGTACC	1 cut(s)	1241
AspLEI	GCGC	2 cut(s)	75, 1042
AspS9I	GGNCC	6 cut(s)	35, 604, 890, 1704, 1792, 2893
AsuC2I	CCSGG	1 cut(s)	2432
AsuHPI	GGTGA	4 cut(s)	223, 506, 512, 2476
AvaI	CYCGRG	2 cut(s)	593, 1794
AvaII	GGWCC	3 cut(s)	890, 1704, 2893
BaeI	ACNNNNGTAYC	4 cut(s)	1233, 1266, 1971, 2004
BanI	GGYRCC	1 cut(s)	1241
BanII	GRGCYC	1 cut(s)	1623
BarI	GAAGNNNNNNTAC	4 cut(s)	1216, 1248, 2999, 3031
BbsI	GAAGAC	6 cut(s)	834, 1836, 2964, 3209, 3213, 3231
Bbv12I	GWGCWC	1 cut(s)	1419
BbvI	GCAGC	5 cut(s)	541, 569, 697, 2856, 3085
BcgI	CGANNNNNNTGC	2 cut(s)	248, 282
BciT130I	CCWGG	9 cut(s)	623, 654, 756, 1421, 2194, 2897, 3035, 3250, 3416
BciVI	GTATCC	2 cut(s)	929, 2392
BclI	TGATCA	4 cut(s)	397, 811, 2209, 2839
BcnI	CCSGG	1 cut(s)	2432
BcoDI	GTCTC	4 cut(s)	121, 329, 2086, 2348
BcuI	ACTAGT	1 cut(s)	3212
BfaI	CTAG	7 cut(s)	204, 521, 707, 2937, 3125, 3213, 3450
BfmI	CTRYAG	9 cut(s)	120, 232, 555, 1146, 1377, 1737, 1809, 2597, 3084
BfrI	CTTAAG	2 cut(s)	248, 3386
BfuAI	ACCTGC	1 cut(s)	1153
BfuI	GTATCC	2 cut(s)	929, 2392
BglII	AGATCT	1 cut(s)	2390
BisI	GCNGC	6 cut(s)	60, 555, 558, 711, 2870, 3099
BlsI	GCNGC	6 cut(s)	61, 556, 559, 712, 2871, 3100
BmcAI	AGTACT	1 cut(s)	1747
Bme18I	GGWCC	3 cut(s)	890, 1704, 2893
BmeRI	GACNNNNNGTC	1 cut(s)	2932
BmeT110I	CYCGRG	2 cut(s)	593, 1794
BmgT120I	GGNCC	6 cut(s)	35, 604, 890, 1704, 1792, 2893
BmiI	GGNNCC	4 cut(s)	744, 1243, 1705, 2564
BmrI	ACTGGG	2 cut(s)	1176, 2374
BmuI	ACTGGG	2 cut(s)	1176, 2374
BoxI	GACNNNNGTC	1 cut(s)	3213
BpiI	GAAGAC	6 cut(s)	834, 1836, 2964, 3209, 3213, 3231
BplI	GAGNNNNNCTC	2 cut(s)	3444, 3476
BpmI	CTGGAG	3 cut(s)	276, 990, 1403
Bpu10I	CCTNAGC	1 cut(s)	738
BpuEI	CTTGAG	2 cut(s)	546, 2394
BpuMI	CCSGG	1 cut(s)	2432
BsaHI	GRCGYC	1 cut(s)	114
BsaJI	CCNNGG	9 cut(s)	594, 621, 1795, 2290, 2454, 2558, 3056, 3177, 3249
BsaWI	WCCGGW	3 cut(s)	581, 856, 1612
Bsc4I	CCNNNNNNNGG	4 cut(s)	932, 2516, 3062, 3183
Bse1I	ACTGG	7 cut(s)	168, 691, 977, 1007, 1182, 2369, 2812
Bse3DI	GCAATG	2 cut(s)	447, 814
BseBI	CCWGG	9 cut(s)	623, 654, 756, 1421, 2194, 2897, 3035, 3250, 3416
BseDI	CCNNGG	9 cut(s)	594, 621, 1795, 2290, 2454, 2558, 3056, 3177, 3249
BseLI	CCNNNNNNNGG	4 cut(s)	932, 2516, 3062, 3183
BseMI	GCAATG	2 cut(s)	447, 814
BseNI	ACTGG	7 cut(s)	168, 691, 977, 1007, 1182, 2369, 2812
BseRI	GAGGAG	2 cut(s)	1226, 3059
BseXI	GCAGC	5 cut(s)	541, 569, 697, 2856, 3085
BseYI	CCCAGC	2 cut(s)	606, 1700
BsgI	GTGCAG	3 cut(s)	291, 1497, 2147
BshFI	GGCC	4 cut(s)	36, 605, 803, 1793
BshNI	GGYRCC	1 cut(s)	1241
BsiHKAI	GWGCWC	1 cut(s)	1419
BsiHKCI	CYCGRG	2 cut(s)	593, 1794
BsiSI	CCGG	5 cut(s)	582, 857, 1259, 1613, 2432
BslFI	GGGAC	6 cut(s)	58, 697, 1042, 1717, 2605, 2874
BslI	CCNNNNNNNGG	4 cut(s)	932, 2516, 3062, 3183
BsmAI	GTCTC	4 cut(s)	121, 329, 2086, 2348
BsmBI	CGTCTC	1 cut(s)	121
BsmFI	GGGAC	6 cut(s)	58, 697, 1042, 1717, 2605, 2874
BsnI	GGCC	4 cut(s)	36, 605, 803, 1793
BsoBI	CYCGRG	2 cut(s)	593, 1794
Bsp1286I	GDGCHC	2 cut(s)	1419, 1623
BspACI	CCGC	6 cut(s)	59, 276, 1513, 1895, 1917, 2459
BspANI	GGCC	4 cut(s)	36, 605, 803, 1793
BspHI	TCATGA	2 cut(s)	1384, 3462
BspLI	GGNNCC	4 cut(s)	744, 1243, 1705, 2564
BspMAI	CTGCAG	6 cut(s)	236, 559, 1150, 1813, 2601, 3088
BspMI	ACCTGC	1 cut(s)	1153
BspPI	GGATC	3 cut(s)	584, 1284, 3047
BspQI	GCTCTTC	2 cut(s)	762, 1115
BspT107I	GGYRCC	1 cut(s)	1241
BspTI	CTTAAG	2 cut(s)	248, 3386
BsrDI	GCAATG	2 cut(s)	447, 814
BsrI	ACTGG	7 cut(s)	168, 691, 977, 1007, 1182, 2369, 2812
BssECI	CCNNGG	9 cut(s)	594, 621, 1795, 2290, 2454, 2558, 3056, 3177, 3249
BssNI	GRCGYC	1 cut(s)	114
BssT1I	CCWWGG	3 cut(s)	2290, 2454, 2558
Bst2UI	CCWGG	9 cut(s)	623, 654, 756, 1421, 2194, 2897, 3035, 3250, 3416
Bst6I	CTCTTC	3 cut(s)	762, 1115, 3162
BstACI	GRCGYC	1 cut(s)	114
BstAFI	CTTAAG	2 cut(s)	248, 3386
BstC8I	GCNNGC	9 cut(s)	77, 261, 805, 1345, 1426, 1537, 1587, 2012, 2905
BstDSI	CCRYGG	1 cut(s)	3056
BstEII	GGTNACC	1 cut(s)	1195
BstENI	CCTNNNNNAGG	1 cut(s)	2514
BstHHI	GCGC	2 cut(s)	75, 1042
BstMAI	GTCTC	4 cut(s)	121, 329, 2086, 2348
BstMWI	GCNNNNNNNGC	1 cut(s)	81
BstNI	CCWGG	9 cut(s)	623, 654, 756, 1421, 2194, 2897, 3035, 3250, 3416
BstNSI	RCATGY	5 cut(s)	1347, 1428, 2143, 2620, 3196
BstPAI	GACNNNNGTC	1 cut(s)	3213
BstPI	GGTNACC	1 cut(s)	1195
BstSFI	CTRYAG	9 cut(s)	120, 232, 555, 1146, 1377, 1737, 1809, 2597, 3084
BstV1I	GCAGC	5 cut(s)	541, 569, 697, 2856, 3085
BstV2I	GAAGAC	6 cut(s)	834, 1836, 2964, 3209, 3213, 3231
BstX2I	RGATCY	1 cut(s)	2390
BstYI	RGATCY	1 cut(s)	2390
BsuI	GTATCC	2 cut(s)	929, 2392
BsuRI	GGCC	4 cut(s)	36, 605, 803, 1793
BtgI	CCRYGG	1 cut(s)	3056
BtsI	GCAGTG	4 cut(s)	241, 1623, 1947, 3081
BveI	ACCTGC	1 cut(s)	1153
Cac8I	GCNNGC	9 cut(s)	77, 261, 805, 1345, 1426, 1537, 1587, 2012, 2905
CaiI	CAGNNNCTG	1 cut(s)	668
CciI	TCATGA	2 cut(s)	1384, 3462
CfoI	GCGC	2 cut(s)	75, 1042
Cfr13I	GGNCC	6 cut(s)	35, 604, 890, 1704, 1792, 2893
CseI	GACGC	2 cut(s)	103, 2708
CsiI	ACCWGGT	1 cut(s)	2192
Csp6I	GTAC	9 cut(s)	411, 650, 854, 1242, 1746, 1901, 2064, 2234, 2246
CviQI	GTAC	9 cut(s)	411, 650, 854, 1242, 1746, 1901, 2064, 2234, 2246
DraI	TTTAAA	2 cut(s)	1767, 1878
DraIII	CACNNNGTG	4 cut(s)	899, 1414, 1453, 3062
DrdI	GACNNNNNNGTC	1 cut(s)	1670
DriI	GACNNNNNGTC	1 cut(s)	2932
DseDI	GACNNNNNNGTC	1 cut(s)	1670
Eam1104I	CTCTTC	3 cut(s)	762, 1115, 3162
Eam1105I	GACNNNNNGTC	1 cut(s)	2932
EarI	CTCTTC	3 cut(s)	762, 1115, 3162
EciI	GGCGGA	2 cut(s)	1884, 2474
Eco130I	CCWWGG	3 cut(s)	2290, 2454, 2558
Eco24I	GRGCYC	1 cut(s)	1623
Eco47I	GGWCC	3 cut(s)	890, 1704, 2893
Eco88I	CYCGRG	2 cut(s)	593, 1794
Eco91I	GGTNACC	1 cut(s)	1195
EcoNI	CCTNNNNNAGG	1 cut(s)	2514
EcoO109I	RGGNCCY	1 cut(s)	2893
EcoO65I	GGTNACC	1 cut(s)	1195
EcoRII	CCWGG	9 cut(s)	621, 652, 754, 1419, 2192, 2895, 3033, 3248, 3414
EcoT14I	CCWWGG	3 cut(s)	2290, 2454, 2558
EcoT22I	ATGCAT	1 cut(s)	2618
EcoT38I	GRGCYC	1 cut(s)	1623
ErhI	CCWWGG	3 cut(s)	2290, 2454, 2558
Esp3I	CGTCTC	1 cut(s)	121
FalI	AAGNNNNNCTT	2 cut(s)	24, 56
FaqI	GGGAC	6 cut(s)	58, 697, 1042, 1717, 2605, 2874
FauNDI	CATATG	1 cut(s)	733
FbaI	TGATCA	4 cut(s)	397, 811, 2209, 2839
FblI	GTMKAC	3 cut(s)	1655, 2644, 3411
Fnu4HI	GCNGC	6 cut(s)	60, 555, 558, 711, 2870, 3099
FriOI	GRGCYC	1 cut(s)	1623
Fsp4HI	GCNGC	6 cut(s)	60, 555, 558, 711, 2870, 3099
FspBI	CTAG	7 cut(s)	204, 521, 707, 2937, 3125, 3213, 3450
GlaI	GCGC	2 cut(s)	74, 1041
GluI	GCNGC	6 cut(s)	60, 555, 558, 711, 2870, 3099
GsaI	CCCAGC	2 cut(s)	610, 1704
GsuI	CTGGAG	3 cut(s)	276, 990, 1403
HaeIII	GGCC	4 cut(s)	36, 605, 803, 1793
HapII	CCGG	5 cut(s)	582, 857, 1259, 1613, 2432
HgaI	GACGC	2 cut(s)	103, 2708
HhaI	GCGC	2 cut(s)	75, 1042
Hin1I	GRCGYC	1 cut(s)	114
Hin6I	GCGC	2 cut(s)	73, 1040
HinP1I	GCGC	2 cut(s)	73, 1040
HincII	GTYRAC	3 cut(s)	2019, 2645, 2698
HindII	GTYRAC	3 cut(s)	2019, 2645, 2698
HindIII	AAGCTT	4 cut(s)	77, 261, 2905, 3354
HpaII	CCGG	5 cut(s)	582, 857, 1259, 1613, 2432
HphI	GGTGA	4 cut(s)	223, 506, 512, 2476
Hpy99I	CGWCG	1 cut(s)	2043
HpyAV	CCTTC	4 cut(s)	1473, 2233, 3025, 3176
HpyCH4IV	ACGT	3 cut(s)	895, 2038, 3408
HpyF10VI	GCNNNNNNNGC	1 cut(s)	81
HpySE526I	ACGT	3 cut(s)	895, 2038, 3408
Hsp92I	GRCGYC	1 cut(s)	114
HspAI	GCGC	2 cut(s)	73, 1040
KpnI	GGTACC	1 cut(s)	1245
Ksp22I	TGATCA	4 cut(s)	397, 811, 2209, 2839
LguI	GCTCTTC	2 cut(s)	762, 1115
LmnI	GCTCC	6 cut(s)	70, 275, 748, 1422, 1931, 2568
Lsp1109I	GCAGC	5 cut(s)	541, 569, 697, 2856, 3085
MabI	ACCWGGT	1 cut(s)	2192
MaeI	CTAG	7 cut(s)	204, 521, 707, 2937, 3125, 3213, 3450
MaeII	ACGT	3 cut(s)	895, 2038, 3408
MaeIII	GTNAC	8 cut(s)	386, 727, 1195, 1750, 2131, 2269, 2500, 3079
MflI	RGATCY	1 cut(s)	2390
MhlI	GDGCHC	2 cut(s)	1419, 1623
MlyI	GAGTC	5 cut(s)	1223, 1450, 1661, 1787, 1885
MmeI	TCCRAC	6 cut(s)	1041, 1140, 1188, 1395, 2616, 2964
Mph1103I	ATGCAT	1 cut(s)	2618
MroXI	GAANNNNTTC	2 cut(s)	1599, 1867
MseI	TTAA	7 cut(s)	249, 993, 1766, 1877, 3332, 3387, 3438
MslI	CAYNNNNRTG	2 cut(s)	1449, 2138
MspCI	CTTAAG	2 cut(s)	248, 3386
MspI	CCGG	5 cut(s)	582, 857, 1259, 1613, 2432
MvaI	CCWGG	9 cut(s)	623, 654, 756, 1421, 2194, 2897, 3035, 3250, 3416
MwoI	GCNNNNNNNGC	1 cut(s)	81
NciI	CCSGG	1 cut(s)	2432
NdeI	CATATG	1 cut(s)	733
NlaIV	GGNNCC	4 cut(s)	744, 1243, 1705, 2564
NmeAIII	GCCGAG	1 cut(s)	3202
NmuCI	GTSAC	3 cut(s)	727, 2131, 3079
NsiI	ATGCAT	1 cut(s)	2618
NspI	RCATGY	5 cut(s)	1347, 1428, 2143, 2620, 3196
PaeI	GCATGC	2 cut(s)	1347, 1428
PagI	TCATGA	2 cut(s)	1384, 3462
PciSI	GCTCTTC	2 cut(s)	762, 1115
PdmI	GAANNNNTTC	2 cut(s)	1599, 1867
PfeI	GAWTC	9 cut(s)	88, 125, 299, 970, 1364, 1814, 2086, 2279, 2438
PflMI	CCANNNNNTGG	1 cut(s)	3062
PfoI	TCCNGGA	2 cut(s)	754, 2895
PkrI	GCNGC	6 cut(s)	61, 556, 559, 712, 2871, 3100
PleI	GAGTC	5 cut(s)	1222, 1449, 1660, 1786, 1885
PpsI	GAGTC	5 cut(s)	1222, 1449, 1660, 1786, 1885
PpuMI	RGGWCCY	1 cut(s)	2893
PshAI	GACNNNNGTC	1 cut(s)	3213
Psp5II	RGGWCCY	1 cut(s)	2893
Psp6I	CCWGG	9 cut(s)	621, 652, 754, 1419, 2192, 2895, 3033, 3248, 3414
PspEI	GGTNACC	1 cut(s)	1195
PspFI	CCCAGC	2 cut(s)	606, 1700
PspGI	CCWGG	9 cut(s)	621, 652, 754, 1419, 2192, 2895, 3033, 3248, 3414
PspN4I	GGNNCC	4 cut(s)	744, 1243, 1705, 2564
PspPI	GGNCC	6 cut(s)	35, 604, 890, 1704, 1792, 2893
PspPPI	RGGWCCY	1 cut(s)	2893
PstI	CTGCAG	6 cut(s)	236, 559, 1150, 1813, 2601, 3088
PstNI	CAGNNNCTG	1 cut(s)	668
PsuI	RGATCY	1 cut(s)	2390
RsaI	GTAC	9 cut(s)	412, 651, 855, 1243, 1747, 1902, 2065, 2235, 2247
RsaNI	GTAC	9 cut(s)	411, 650, 854, 1242, 1746, 1901, 2064, 2234, 2246
RseI	CAYNNNNRTG	2 cut(s)	1449, 2138
SalI	GTCGAC	1 cut(s)	2643
SapI	GCTCTTC	2 cut(s)	762, 1115
SaqAI	TTAA	7 cut(s)	249, 993, 1766, 1877, 3332, 3387, 3438
SatI	GCNGC	6 cut(s)	60, 555, 558, 711, 2870, 3099
Sau96I	GGNCC	6 cut(s)	35, 604, 890, 1704, 1792, 2893
SbfI	CCTGCAGG	1 cut(s)	1150
ScaI	AGTACT	1 cut(s)	1747
SchI	GAGTC	5 cut(s)	1223, 1450, 1661, 1787, 1885
SdaI	CCTGCAGG	1 cut(s)	1150
SduI	GDGCHC	2 cut(s)	1419, 1623
SexAI	ACCWGGT	1 cut(s)	2192
SfcI	CTRYAG	9 cut(s)	120, 232, 555, 1146, 1377, 1737, 1809, 2597, 3084
SinI	GGWCC	3 cut(s)	890, 1704, 2893
SmiMI	CAYNNNNRTG	2 cut(s)	1449, 2138
SmlI	CTYRAG	4 cut(s)	248, 561, 2409, 3386
SmoI	CTYRAG	4 cut(s)	248, 561, 2409, 3386
SpeI	ACTAGT	1 cut(s)	3212
SphI	GCATGC	2 cut(s)	1347, 1428
Sse8387I	CCTGCAGG	1 cut(s)	1150
SsiI	CCGC	6 cut(s)	59, 276, 1513, 1895, 1917, 2459
SspMI	CTAG	7 cut(s)	204, 521, 707, 2937, 3125, 3213, 3450
StyI	CCWWGG	3 cut(s)	2290, 2454, 2558
TaiI	ACGT	3 cut(s)	898, 2041, 3411
TaqI	TCGA	4 cut(s)	282, 2441, 2644, 2727
TatI	WGTACW	3 cut(s)	410, 1745, 2063
TauI	GCSGC	1 cut(s)	62
TfiI	GAWTC	9 cut(s)	88, 125, 299, 970, 1364, 1814, 2086, 2279, 2438
Tru1I	TTAA	7 cut(s)	249, 993, 1766, 1877, 3332, 3387, 3438
Tru9I	TTAA	7 cut(s)	249, 993, 1766, 1877, 3332, 3387, 3438
TseFI	GTSAC	3 cut(s)	727, 2131, 3079
TseI	GCWGC	5 cut(s)	554, 557, 710, 2869, 3098
Tsp45I	GTSAC	3 cut(s)	727, 2131, 3079
TspGWI	ACGGA	2 cut(s)	2245, 3073
Van91I	CCANNNNNTGG	1 cut(s)	3062
Vha464I	CTTAAG	2 cut(s)	248, 3386
VpaK11BI	GGWCC	3 cut(s)	890, 1704, 2893
XagI	CCTNNNNNAGG	1 cut(s)	2514
XbaI	TCTAGA	2 cut(s)	203, 520
XceI	RCATGY	5 cut(s)	1347, 1428, 2143, 2620, 3196
XmiI	GTMKAC	3 cut(s)	1655, 2644, 3411
XmnI	GAANNNNTTC	2 cut(s)	1599, 1867
XspI	CTAG	7 cut(s)	204, 521, 707, 2937, 3125, 3213, 3450
ZrmI	AGTACT	1 cut(s)	1747
Zsp2I	ATGCAT	1 cut(s)	2618

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.