Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0004703

Physical Location & Seq

Genomic Coordinates Forward (+)

9538179 .. 9546191

Gene Structure 8013 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1050200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3834 bp

ATGGACGGGGCTAATTGTTCGACCCAAGTGAGCAGAGAAGGTGGCGGTGCTGGAAATGGAGGTTTGGAAAATGTGACTCCTCGGGTAATAAGGACAGTTGGTAGAGCTGCTTCGAGTTATTCGGGGAGTAATTCAACGCAGACAACACCGTCCAAGAGTGTGTCGAAGCCTCCGAATTTGGGTTTTCAGAATAAGGCTGATGGAAGTGTTGGTCCTCGAGTAGGGAACTTTGCTGCATTGTATAAAGGGATTCCCATTTCTTACAGTCCGTATACGGTGGTTAATACAGTGGAAGTGCCTCACTTTGATCTCAAGGAAGATCCATCTTTCTGGATAGATCACAATGTACAGGTTCTCATTCGTGTTCGTCCTCTCAATAGCATGGAGCGAAGTTTACATGGTAACAGCAGGTGTTTGAAGCAAGAAAGTACTCAGACAATTTCCTGGATTGGGCAACCAGAAAGTCGATTTACATTTGATCATGTAGCCTGTGAAACAGTTGACCAGATGCTTTTCAGGATGGCTGGTCTACCCATGGTAGAGAATTGCTTGTCGGGATATAATAGTTGTATGTTTGCCTACGGTCAGACAGGAAGTGGTAAAACACATACAATGCTTGGGGAGATTGACGATTTAGAAACGAAGCCCGGTCCAAATCGTGGAATGACACCCCAAATTTTCGAGTTTTTATTAGCAAGGATCCAAGCTGAAGAGGAAATCCGGAGGGATGAGAAACTAAAATACAATTGCAAGTGCTCTTTCTTAGAAATTTACAATGAACAAATTACGGATCTCCTTGATCCGTCATCTACCAATTTGCTTCTGCGAGAAGATGTTAAGAAGGGTGTTTATGTAGAAAATCTCTCTGAATTTGAAGTCTATATTGTGAGCGATATCCTCAGGCTTCTCATTCAGGGTTCTTCAAATAGGAAAGTTGCTGCAACAAATATGAATAGAGAAAGCAGTCGTTCGCACAGTGTCTTTACTTGTGTAATTGAGAGTAGGTGGGAAAAAGATTCCACAACTAACCTACGGTTTGCTAGACTAAACCTAGTTGATCTGGCTGGTTCAGAAAGTCATGTTATAATGGTTCTAGTGGATATGGCACATGGGAAGCTGAAGCATGTACCTTACAGAGACTCTAGGCTAACCTTCCTACCACAGGACTCACTTGGGGGAAACTCAAAAACAATGATCATTGCAAATGTCAGCCCATCTATCTGTTGTGCCGCTGAAACACTGAGTACCCTTAAGTTTGCTCAGAGAGCAAAACTTATTCAAAACAATGCTGTGGTGAATGAGGATGCCACAGGCGACATCATGGCCCTACAACACCAAATACGGCTCCTCAAGGTGGAGCAAATTTCCGTTCTTAAGCGTCATAATGTCTCCCGACCTTTGTCGTTTTGTTCTACCAATTTTGAAGACACACAAAGAGTCCGAAAAATTGATTGTGGTGAAAACGTGTGTGATATGGACCTAGAGTGTGATGATGATTTTCTTGAAAATCAATCAAAAGGAGCTGTGAGACTTTCCACCAAAAAGTTAAAATATTTGGAGACAACGCTTGCGGGTGCCTTGAGAAGACAGCAGATGGCAGAAGCTACCATCAAGCAACTTGAAGCTGAAAATGAGCAGCTGAACCGTTTGGTTTGTCAAATTGAGGCAGACACCAGGGGCACTGAAATGATGCTAAGGTTTCGAGAAGACAAAATTCAGACAATGGAGTCACTTCTTAGTGGTTCTATTCCCGTTGAGTCTTATTTACTGGATGAGAACAGAGCACTCTCTGAAGAGATTCAGCTAATTCAAGCAAAACTTGATAAGAATCCTGAAGTCACTCGGTTTGCATTGGAGAACATAAGGCTTTTGGACCAACTTAGAAGAGAGCTCTTGTACGAGGTATCTAAACTGCGAGATCAGTTGCTTCAATTTCTTGATGGACACTCTAAGCAGCATGGCAACCCAAATTTTAGTATGAAAACTCAGGAAACCGTATGCACCGACAGAGATAGTGATTCTTTCAATACGGAGTTGAAAAATACTGTCCATGAGTTAGAGGAATGCAGGCGCAGCTTAAATACTTGTTTGAAGGAAAATGAAAAACTCAACAGGGAAATTGATGATCTGCAGACAATGCTTAACAAGCTTAAGTGTACACCCATTGACCAAAATGATTGTGTAGCTTTAAAGGTGCATAATGCTGTTCCCATTGGGGAGATGGAACCGAAGCTTGACCCTCAAATAAAACATGCGCAAGAAATTTTAAATTTACAGCTGGAACTGGATATAGTGAATATCATTTTGAAAGAAGAGAGGACTTCTCAAGAGGAGAGGGTACATTTCTTAAATAAGGATCTTCAGCTGGCAAATGAAGAACTTTTTGTGATAAGCAAACAGCGTAATGATGCAAGCAGCCAATTGGAGGAGGCTAAATCTGTTATTGAAGCTCTCGAGTCTCAGCAACTTCTGTCAATCAATGAGCTTGAGGACCTGAGCAAGCTTTTGAGTGAACGGGAGCTTGAATTGAGGGCTCTAAAGGAGCAACAGACGTTTAAGGAGTTTAGGGATTTATCACCTTTGAACTGCTCAAACAACCAAGACTTACCATTACAGGGAAATTTGAAGAGGATGCTTGATTCCCTTGAAAACGCCAAGAGACTGAATACGTCCCTAAGTGATCGTGCATTTCAAGTGTCAAATGAAGAAGAAATGGATGAAGTGCATAAGCAGGTTGAGATTGAAACTGCAGAGGTGATTGTCTGTATGCAGGAAGAACTTGGCATGCTTCAGCAGCAAGTTCAAAATGGCCATTTAAAAGAATTGGAGATGAACAAGAATGTAATGATCTTGGAAACAGAATTGAAGGAGGTGCAAGAAAAGTTTTACATGTTGAATGAAGATAATGAACGGTTAAGTAAAGAGCTTGAGGAGAAAGATGGGGAAGTAAGAATGTTGTCGCAAGAATGGACATTATTGTCCAGTGAGATTGAAGAGGTTCTGTCTGATGGGTGTGAGGAACTAGATGATGCCTCTGATCAACTTAACCTTATATCCGATTCCTTTCCACAGAAAAGGATTTGGATCTCCGAACAAGTTGGAAGAATCATGCGAATCATCTCTGAAAAGGAATTTTTGATTAATGAACTGAGGAAGTGTTTGAAGGATGCAACCAACAAGAAATGTGATGTGGAGTGCATGCTGAATTCTATGAGAGGAGCAACATTGGCTATAACTGAAGCACGTGAACAGGAGTGTTGTGAAAAGGAGAAAGAAATGATCATGTTGACAACGCAGTTGAATGCAGAGTCATCCATAGTGGAAAAGCTTGAGAATCGAGTGAAGGTACTAGAAAAGCAGATCAGGAAAACATCAGTCTGTGCAACAGTTGCTTTCGTTGTTGTGGACAGATTAGCAAAGATGAATCTTAGTAATGAAGATGCATTAAAGTGTAAGAGTATTCAGCTTAGTGAATCAGAAGACTTGATCTCAACGAAGGTTGCCATTCTCAGTGATCGGGAGTCTGTGATAGCAGAAGCAGAGATAAAAGTTCAGTCTTTAAATGCGGAAGTTGAAGAGTTGAAGAGAACCTGTGCCAATCTAAGATGGGTGCTTTCTGAGGAGCGGGAACATGCATTCATTATAGAACAAAAGCTAGAAGATGTCGAAGAGAAAAACATTTTGATGACAAAGGAGAAACTAGCTGAGCTAAAAACTGGCTTATCCACATTTATGTCATGCATGAACACAAATGTGGAGCATCATACAAGCTCTGAAGGGAAGGATTCACAAGTAGCTTCTAAGTCTCCAAAGGAGAGGGTGGTGGATGGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000278 GO:0000281 GO:0000910 GO:0000911 GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005871 GO:0005875 GO:0006928 GO:0007017 GO:0007018 GO:0007049 GO:0008150 GO:0009987 GO:0015630 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022402 GO:0032506 GO:0032991 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0051301 GO:0061640 GO:1902410 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kinesin

Protein Analysis

1278

Amino Acids

145.05

Weight (kDa)

5.06

Isoelectric Point (pI)

44.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Microtub_bd	PF16796	115 - 266	9.9e-21	Microtubule binding
Kinesin	PF00225	121 - 359	1.4e-65	Kinesin motor domain
Kinesin	PF00225	360 - 426	2e-19	Kinesin motor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0004703)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G19050
fragaria_vesca	FvH4_2g07030 FvH4_2g07030 FvH4_2g07030 FvH4_2g07030 FvH4_2g07030 FvH4_2g07030
malus_domestica	MD00G1050200.v1.1 MD10G1088400.v1.1
prunus_persica	Prupe.8G115500_v2.0.a1
pyrus_communis	pycom05g06710 pycom05g06720 pycom10g07100 pycom10g07120 pycom10g07130
rosa_chinensis	RchiOBHm_Chr6g0259391
rosa_laevigata	RLG00000014535
rosa_multiflora	Rmu_sc0012054.1_g000003 Rmu_sc0023331.1_g000001 Rmu_sc0023332.1_g000001 Rmu_sc0037344.1_g000001
rosa_roxburghii	Rroxscaffold_2G00123360 Rroxscaffold_5G00349410 Rroxscaffold_7G00206650
rosa_rugosa	Rorug05G0588700
rosa_samantha	Rh6AG104100 Rh6AG104200 Rh6BG097400 Rh6CG093500 Rh6DG087300
rosa_wichuraiana	Rw6G009000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1085
AarI	CACCTGC	1 cut(s)	399
AasI	GACNNNNNNGTC	3 cut(s)	148, 1726, 2977
Acc16I	TGCGCA	1 cut(s)	2256
Acc36I	ACCTGC	2 cut(s)	399, 2722
AccB1I	GGYRCC	1 cut(s)	1574
AccB7I	CCANNNNNTGG	1 cut(s)	659
AccBSI	CCGCTC	1 cut(s)	3625
AccI	GTMKAC	2 cut(s)	272, 529
AccIII	TCCGGA	1 cut(s)	720
AciI	CCGC	5 cut(s)	45, 1230, 1571, 3566, 3625
AclWI	GGATC	7 cut(s)	314, 694, 707, 794, 798, 2364, 3092
AcoI	YGGCCR	1 cut(s)	2809
AcuI	CTGAAG	8 cut(s)	729, 1139, 1812, 1854, 2345, 2774, 3258, 3795
AcvI	CACGTG	1 cut(s)	3245
AfaI	GTAC	8 cut(s)	348, 430, 1128, 1246, 1898, 2158, 2340, 3348
AfiI	CCNNNNNNNGG	8 cut(s)	179, 221, 450, 659, 1162, 1354, 2425, 2615
AflII	CTTAAG	3 cut(s)	1250, 1373, 2150
AflIII	ACRYGT	2 cut(s)	1464, 2889
AjnI	CCWGG	2 cut(s)	443, 1673
AleI	CACNNNNGTG	1 cut(s)	3752
Alw21I	GWGCWC	3 cut(s)	758, 1786, 1893
Alw26I	GTCTC	7 cut(s)	1131, 1393, 1522, 1553, 2463, 2653, 3810
AlwI	GGATC	7 cut(s)	314, 694, 707, 794, 798, 2364, 3092
Ama87I	CYCGRG	3 cut(s)	81, 216, 2453
Aor13HI	TCCGGA	1 cut(s)	720
AoxI	GGCC	2 cut(s)	1323, 2809
ApeKI	GCWGC	8 cut(s)	107, 233, 938, 1636, 1954, 2073, 2415, 2794
AseI	ATTAAT	1 cut(s)	3141
Asp700I	GAANNNNTTC	2 cut(s)	1797, 2997
AspLEI	GCGC	2 cut(s)	2073, 2257
AspS9I	GGNCC	6 cut(s)	212, 650, 1324, 1477, 1873, 2491
AsuC2I	CCSGG	1 cut(s)	648
AsuHPI	GGTGA	4 cut(s)	1306, 1469, 2568, 2767
AvaI	CYCGRG	3 cut(s)	81, 216, 2453
AvaII	GGWCC	5 cut(s)	212, 650, 1477, 1873, 2491
AxyI	CCTNAGG	1 cut(s)	899
BaeGI	GKGCMC	1 cut(s)	1682
BalI	TGGCCA	1 cut(s)	2811
BamHI	GGATCC	1 cut(s)	699
BanI	GGYRCC	1 cut(s)	1574
BanII	GRGCYC	2 cut(s)	1893, 2536
BarI	GAAGNNNNNNTAC	2 cut(s)	94, 126
BbrPI	CACGTG	1 cut(s)	3245
BbsI	GAAGAC	4 cut(s)	1431, 1591, 1713, 3486
Bbv12I	GWGCWC	3 cut(s)	758, 1786, 1893
BbvI	GCAGC	8 cut(s)	94, 220, 925, 1648, 1966, 2085, 2427, 2806
BceAI	ACGGC	1 cut(s)	1358
BciT130I	CCWGG	2 cut(s)	445, 1675
BclI	TGATCA	4 cut(s)	478, 1194, 3037, 3279
BcnI	CCSGG	1 cut(s)	648
BcoDI	GTCTC	7 cut(s)	1131, 1393, 1522, 1553, 2463, 2653, 3810
BfaI	CTAG	9 cut(s)	1041, 1052, 1094, 1143, 1481, 3023, 3350, 3656, 3701
BfmI	CTRYAG	2 cut(s)	2129, 2748
BfrI	CTTAAG	3 cut(s)	1250, 1373, 2150
BfuAI	ACCTGC	2 cut(s)	399, 2722
BisI	GCNGC	9 cut(s)	108, 234, 939, 1230, 1637, 1955, 2074, 2416, 2795
BlpI	GCTNAGC	1 cut(s)	3705
BlsI	GCNGC	9 cut(s)	109, 235, 940, 1231, 1638, 1956, 2075, 2417, 2796
BmcAI	AGTACT	1 cut(s)	430
Bme1390I	CCNGG	3 cut(s)	445, 648, 1675
Bme18I	GGWCC	5 cut(s)	212, 650, 1477, 1873, 2491
BmeT110I	CYCGRG	3 cut(s)	81, 216, 2453
BmgT120I	GGNCC	6 cut(s)	212, 650, 1324, 1477, 1873, 2491
BmiI	GGNNCC	4 cut(s)	701, 1346, 1576, 2226
BmrFI	CCNGG	3 cut(s)	445, 648, 1675
BmsI	GCATC	9 cut(s)	498, 1294, 1680, 2398, 2622, 3019, 3157, 3430, 3769
BoxI	GACNNNNGTC	1 cut(s)	1399
BpiI	GAAGAC	4 cut(s)	1431, 1591, 1713, 3486
BplI	GAGNNNNNCTC	2 cut(s)	2308, 2340
Bpu10I	CCTNAGC	2 cut(s)	1694, 2495
Bpu1102I	GCTNAGC	1 cut(s)	3705
BpuEI	CTTGAG	7 cut(s)	296, 1334, 1600, 2310, 2507, 2948, 3350
BpuMI	CCSGG	1 cut(s)	648
BsaAI	YACGTR	1 cut(s)	3245
BsaBI	GATNNNNATC	2 cut(s)	1827, 3083
BsaJI	CCNNGG	3 cut(s)	80, 534, 1674
BsaWI	WCCGGW	1 cut(s)	720
Bsc4I	CCNNNNNNNGG	8 cut(s)	179, 221, 450, 659, 1162, 1354, 2425, 2615
Bse1I	ACTGG	4 cut(s)	1773, 2289, 2982, 3721
Bse21I	CCTNAGG	1 cut(s)	899
Bse3DI	GCAATG	1 cut(s)	1197
Bse8I	GATNNNNATC	2 cut(s)	1827, 3083
BseAI	TCCGGA	1 cut(s)	720
BseBI	CCWGG	2 cut(s)	445, 1675
BseDI	CCNNGG	3 cut(s)	80, 534, 1674
BseGI	GGATG	9 cut(s)	525, 733, 1309, 1777, 2637, 2722, 3172, 3311, 3832
BseJI	GATNNNNATC	2 cut(s)	1827, 3083
BseLI	CCNNNNNNNGG	8 cut(s)	179, 221, 450, 659, 1162, 1354, 2425, 2615
BseMI	GCAATG	1 cut(s)	1197
BseNI	ACTGG	4 cut(s)	1773, 2289, 2982, 3721
BseRI	GAGGAG	7 cut(s)	69, 1337, 2345, 2441, 2945, 3231, 3635
BseSI	GKGCMC	1 cut(s)	1682
BseXI	GCAGC	8 cut(s)	94, 220, 925, 1648, 1966, 2085, 2427, 2806
BshFI	GGCC	2 cut(s)	1325, 2811
BshNI	GGYRCC	1 cut(s)	1574
BsiHKAI	GWGCWC	3 cut(s)	758, 1786, 1893
BsiHKCI	CYCGRG	3 cut(s)	81, 216, 2453
BsiSI	CCGG	2 cut(s)	648, 721
BslFI	GGGAC	1 cut(s)	2656
BslI	CCNNNNNNNGG	8 cut(s)	179, 221, 450, 659, 1162, 1354, 2425, 2615
BsmAI	GTCTC	7 cut(s)	1131, 1393, 1522, 1553, 2463, 2653, 3810
BsmFI	GGGAC	1 cut(s)	2656
BsmI	GAATGC	3 cut(s)	2069, 3307, 3635
BsnI	GGCC	2 cut(s)	1325, 2811
BsoBI	CYCGRG	3 cut(s)	81, 216, 2453
Bsp1286I	GDGCHC	5 cut(s)	758, 1682, 1786, 1893, 2536
Bsp13I	TCCGGA	1 cut(s)	720
Bsp1407I	TGTACA	2 cut(s)	346, 2156
Bsp1720I	GCTNAGC	1 cut(s)	3705
Bsp19I	CCATGG	1 cut(s)	534
BspACI	CCGC	5 cut(s)	45, 1230, 1571, 3566, 3625
BspANI	GGCC	2 cut(s)	1325, 2811
BspEI	TCCGGA	1 cut(s)	720
BspLI	GGNNCC	4 cut(s)	701, 1346, 1576, 2226
BspMAI	CTGCAG	2 cut(s)	2133, 2752
BspMI	ACCTGC	2 cut(s)	399, 2722
BspPI	GGATC	7 cut(s)	314, 694, 707, 794, 798, 2364, 3092
BspT107I	GGYRCC	1 cut(s)	1574
BspTI	CTTAAG	3 cut(s)	1250, 1373, 2150
BsrBI	CCGCTC	1 cut(s)	3625
BsrDI	GCAATG	1 cut(s)	1197
BsrGI	TGTACA	2 cut(s)	346, 2156
BsrI	ACTGG	4 cut(s)	1773, 2289, 2982, 3721
BssECI	CCNNGG	3 cut(s)	80, 534, 1674
BssNAI	GTATAC	1 cut(s)	273
BssT1I	CCWWGG	1 cut(s)	534
Bst1107I	GTATAC	1 cut(s)	273
Bst2UI	CCWGG	2 cut(s)	445, 1675
Bst6I	CTCTTC	9 cut(s)	705, 1788, 1879, 2307, 2621, 2988, 3570, 3578, 3663
BstAFI	CTTAAG	3 cut(s)	1250, 1373, 2150
BstAPI	GCANNNNNTGC	2 cut(s)	2137, 3389
BstAUI	TGTACA	2 cut(s)	346, 2156
BstBAI	YACGTR	1 cut(s)	3245
BstC8I	GCNNGC	7 cut(s)	1569, 2069, 2367, 2413, 2501, 2786, 3200
BstDSI	CCRYGG	1 cut(s)	534
BstENI	CCTNNNNNAGG	2 cut(s)	219, 1160
BstF5I	GGATG	9 cut(s)	525, 733, 1309, 1777, 2637, 2722, 3172, 3311, 3832
BstHHI	GCGC	2 cut(s)	2073, 2257
BstMAI	GTCTC	7 cut(s)	1131, 1393, 1522, 1553, 2463, 2653, 3810
BstMWI	GCNNNNNNNGC	7 cut(s)	1265, 2073, 2137, 2146, 2794, 3227, 3389
BstNI	CCWGG	2 cut(s)	445, 1675
BstNSI	RCATGY	6 cut(s)	1127, 2255, 2788, 2893, 3202, 3635
BstPAI	GACNNNNGTC	1 cut(s)	1399
BstSCI	CCNGG	3 cut(s)	443, 646, 1673
BstSFI	CTRYAG	2 cut(s)	2129, 2748
BstSLI	GKGCMC	1 cut(s)	1682
BstV1I	GCAGC	8 cut(s)	94, 220, 925, 1648, 1966, 2085, 2427, 2806
BstV2I	GAAGAC	4 cut(s)	1431, 1591, 1713, 3486
BstX2I	RGATCY	5 cut(s)	319, 699, 790, 2356, 3084
BstXI	CCANNNNNNTGG	1 cut(s)	330
BstYI	RGATCY	5 cut(s)	319, 699, 790, 2356, 3084
BstZ17I	GTATAC	1 cut(s)	273
Bsu36I	CCTNAGG	1 cut(s)	899
BsuRI	GGCC	2 cut(s)	1325, 2811
BtgI	CCRYGG	1 cut(s)	534
BtsCI	GGATG	9 cut(s)	525, 733, 1309, 1777, 2637, 2722, 3172, 3311, 3832
BtsIMutI	CAGTG	6 cut(s)	294, 982, 1238, 1680, 2989, 3517
BveI	ACCTGC	2 cut(s)	399, 2722
Cac8I	GCNNGC	7 cut(s)	1569, 2069, 2367, 2413, 2501, 2786, 3200
CfoI	GCGC	2 cut(s)	2073, 2257
Cfr13I	GGNCC	6 cut(s)	212, 650, 1324, 1477, 1873, 2491
CseI	GACGC	1 cut(s)	1367
Csp6I	GTAC	8 cut(s)	347, 429, 1127, 1245, 1897, 2157, 2339, 3347
CviQI	GTAC	8 cut(s)	347, 429, 1127, 1245, 1897, 2157, 2339, 3347
DraI	TTTAAA	4 cut(s)	2190, 2268, 2817, 3561
DrdI	GACNNNNNNGTC	3 cut(s)	148, 1726, 2977
DseDI	GACNNNNNNGTC	3 cut(s)	148, 1726, 2977
EaeI	YGGCCR	1 cut(s)	2809
Eam1104I	CTCTTC	9 cut(s)	705, 1788, 1879, 2307, 2621, 2988, 3570, 3578, 3663
EarI	CTCTTC	9 cut(s)	705, 1788, 1879, 2307, 2621, 2988, 3570, 3578, 3663
Ecl136II	GAGCTC	1 cut(s)	1891
Eco130I	CCWWGG	1 cut(s)	534
Eco24I	GRGCYC	2 cut(s)	1893, 2536
Eco32I	GATATC	1 cut(s)	895
Eco47I	GGWCC	5 cut(s)	212, 650, 1477, 1873, 2491
Eco53kI	GAGCTC	1 cut(s)	1891
Eco57I	CTGAAG	8 cut(s)	729, 1139, 1812, 1854, 2345, 2774, 3258, 3795
Eco72I	CACGTG	1 cut(s)	3245
Eco81I	CCTNAGG	1 cut(s)	899
Eco88I	CYCGRG	3 cut(s)	81, 216, 2453
EcoICRI	GAGCTC	1 cut(s)	1891
EcoNI	CCTNNNNNAGG	2 cut(s)	219, 1160
EcoO109I	RGGNCCY	1 cut(s)	2491
EcoRI	GAATTC	1 cut(s)	3205
EcoRII	CCWGG	2 cut(s)	443, 1673
EcoRV	GATATC	1 cut(s)	895
EcoT14I	CCWWGG	1 cut(s)	534
EcoT22I	ATGCAT	3 cut(s)	3445, 3637, 3743
EcoT38I	GRGCYC	2 cut(s)	1893, 2536
ErhI	CCWWGG	1 cut(s)	534
FalI	AAGNNNNNCTT	2 cut(s)	1803, 1835
FaqI	GGGAC	1 cut(s)	2656
FauI	CCCGC	2 cut(s)	1564, 3618
FbaI	TGATCA	4 cut(s)	478, 1194, 3037, 3279
FblI	GTMKAC	2 cut(s)	272, 529
Fnu4HI	GCNGC	9 cut(s)	108, 234, 939, 1230, 1637, 1955, 2074, 2416, 2795
FokI	GGATG	8 cut(s)	532, 740, 1316, 1784, 2644, 2729, 3179, 3298
FriOI	GRGCYC	2 cut(s)	1893, 2536
Fsp4HI	GCNGC	9 cut(s)	108, 234, 939, 1230, 1637, 1955, 2074, 2416, 2795
FspBI	CTAG	9 cut(s)	1041, 1052, 1094, 1143, 1481, 3023, 3350, 3656, 3701
FspI	TGCGCA	1 cut(s)	2256
GlaI	GCGC	2 cut(s)	2072, 2256
GluI	GCNGC	9 cut(s)	108, 234, 939, 1230, 1637, 1955, 2074, 2416, 2795
HaeIII	GGCC	2 cut(s)	1325, 2811
HapII	CCGG	2 cut(s)	648, 721
HgaI	GACGC	1 cut(s)	1367
HhaI	GCGC	2 cut(s)	2073, 2257
Hin6I	GCGC	2 cut(s)	2071, 2255
HinP1I	GCGC	2 cut(s)	2071, 2255
HincII	GTYRAC	2 cut(s)	502, 3288
HindII	GTYRAC	2 cut(s)	502, 3288
HindIII	AAGCTT	4 cut(s)	2147, 2231, 2501, 3326
HpaII	CCGG	2 cut(s)	648, 721
HphI	GGTGA	4 cut(s)	1306, 1469, 2568, 2767
HpyCH4IV	ACGT	4 cut(s)	1464, 2552, 2669, 3244
HpyF10VI	GCNNNNNNNGC	7 cut(s)	1265, 2073, 2137, 2146, 2794, 3227, 3389
HpySE526I	ACGT	4 cut(s)	1464, 2552, 2669, 3244
HspAI	GCGC	2 cut(s)	2071, 2255
Kpn2I	TCCGGA	1 cut(s)	720
Ksp22I	TGATCA	4 cut(s)	478, 1194, 3037, 3279
LmnI	GCTCC	9 cut(s)	385, 1350, 1357, 1520, 2518, 2542, 3218, 3622, 3757
Lsp1109I	GCAGC	8 cut(s)	94, 220, 925, 1648, 1966, 2085, 2427, 2806
LweI	GCATC	9 cut(s)	498, 1294, 1680, 2398, 2622, 3019, 3157, 3430, 3769
MaeI	CTAG	9 cut(s)	1041, 1052, 1094, 1143, 1481, 3023, 3350, 3656, 3701
MaeII	ACGT	4 cut(s)	1464, 2552, 2669, 3244
MaeIII	GTNAC	4 cut(s)	73, 401, 1728, 1837
MbiI	CCGCTC	1 cut(s)	3625
MfeI	CAATTG	2 cut(s)	745, 2420
MflI	RGATCY	5 cut(s)	319, 699, 790, 2356, 3084
MhlI	GDGCHC	5 cut(s)	758, 1682, 1786, 1893, 2536
MlsI	TGGCCA	1 cut(s)	2811
MluNI	TGGCCA	1 cut(s)	2811
MlyI	GAGTC	9 cut(s)	70, 1133, 1160, 1446, 1736, 1766, 2465, 3317, 3530
MmeI	TCCRAC	1 cut(s)	3079
Mox20I	TGGCCA	1 cut(s)	2811
Mph1103I	ATGCAT	3 cut(s)	3445, 3637, 3743
MroI	TCCGGA	1 cut(s)	720
MroXI	GAANNNNTTC	2 cut(s)	1797, 2997
MscI	TGGCCA	1 cut(s)	2811
MslI	CAYNNNNRTG	5 cut(s)	1685, 3448, 3683, 3731, 3752
Msp20I	TGGCCA	1 cut(s)	2811
MspA1I	CMGCKG	4 cut(s)	1232, 1639, 2278, 2365
MspCI	CTTAAG	3 cut(s)	1250, 1373, 2150
MspI	CCGG	2 cut(s)	648, 721
MspR9I	CCNGG	3 cut(s)	445, 648, 1675
MunI	CAATTG	2 cut(s)	745, 2420
Mva1269I	GAATGC	3 cut(s)	2069, 3307, 3635
MvaI	CCWGG	2 cut(s)	445, 1675
MwoI	GCNNNNNNNGC	7 cut(s)	1265, 2073, 2137, 2146, 2794, 3227, 3389
NciI	CCSGG	1 cut(s)	648
NcoI	CCATGG	1 cut(s)	534
NlaIV	GGNNCC	4 cut(s)	701, 1346, 1576, 2226
NmuCI	GTSAC	3 cut(s)	73, 1728, 1837
NsbI	TGCGCA	1 cut(s)	2256
NsiI	ATGCAT	3 cut(s)	3445, 3637, 3743
NspI	RCATGY	6 cut(s)	1127, 2255, 2788, 2893, 3202, 3635
OliI	CACNNNNGTG	1 cut(s)	3752
PaeI	GCATGC	2 cut(s)	2788, 3202
PaeR7I	CTCGAG	2 cut(s)	216, 2453
PaqCI	CACCTGC	1 cut(s)	399
PciI	ACATGT	1 cut(s)	2889
PcsI	WCGNNNNNNNCGW	1 cut(s)	170
PctI	GAATGC	3 cut(s)	2069, 3307, 3635
PdmI	GAANNNNTTC	2 cut(s)	1797, 2997
PflMI	CCANNNNNTGG	1 cut(s)	659
PfoI	TCCNGGA	1 cut(s)	443
PkrI	GCNGC	9 cut(s)	109, 235, 940, 1231, 1638, 1956, 2075, 2417, 2796
PleI	GAGTC	9 cut(s)	70, 1133, 1160, 1445, 1735, 1765, 2464, 3316, 3529
PmaCI	CACGTG	1 cut(s)	3245
PmlI	CACGTG	1 cut(s)	3245
PpsI	GAGTC	9 cut(s)	70, 1133, 1160, 1445, 1735, 1765, 2464, 3316, 3529
Ppu21I	YACGTR	1 cut(s)	3245
PpuMI	RGGWCCY	1 cut(s)	2491
PscI	ACATGT	1 cut(s)	2889
PshAI	GACNNNNGTC	1 cut(s)	1399
PshBI	ATTAAT	1 cut(s)	3141
PsiI	TTATAA	1 cut(s)	1085
Psp124BI	GAGCTC	1 cut(s)	1893
Psp5II	RGGWCCY	1 cut(s)	2491
Psp6I	CCWGG	2 cut(s)	443, 1673
PspCI	CACGTG	1 cut(s)	3245
PspGI	CCWGG	2 cut(s)	443, 1673
PspN4I	GGNNCC	4 cut(s)	701, 1346, 1576, 2226
PspPI	GGNCC	6 cut(s)	212, 650, 1324, 1477, 1873, 2491
PspPPI	RGGWCCY	1 cut(s)	2491
PspXI	VCTCGAGB	1 cut(s)	216
PstI	CTGCAG	2 cut(s)	2133, 2752
PsuI	RGATCY	5 cut(s)	319, 699, 790, 2356, 3084
PvuII	CAGCTG	3 cut(s)	1639, 2278, 2365
RsaI	GTAC	8 cut(s)	348, 430, 1128, 1246, 1898, 2158, 2340, 3348
RsaNI	GTAC	8 cut(s)	347, 429, 1127, 1245, 1897, 2157, 2339, 3347
RseI	CAYNNNNRTG	5 cut(s)	1685, 3448, 3683, 3731, 3752
SacI	GAGCTC	1 cut(s)	1893
SatI	GCNGC	9 cut(s)	108, 234, 939, 1230, 1637, 1955, 2074, 2416, 2795
Sau96I	GGNCC	6 cut(s)	212, 650, 1324, 1477, 1873, 2491
ScaI	AGTACT	1 cut(s)	430
SchI	GAGTC	9 cut(s)	70, 1133, 1160, 1446, 1736, 1766, 2465, 3317, 3530
ScrFI	CCNGG	3 cut(s)	445, 648, 1675
SduI	GDGCHC	5 cut(s)	758, 1682, 1786, 1893, 2536
SfaNI	GCATC	9 cut(s)	498, 1294, 1680, 2398, 2622, 3019, 3157, 3430, 3769
SfcI	CTRYAG	2 cut(s)	2129, 2748
Sfr274I	CTCGAG	2 cut(s)	216, 2453
SinI	GGWCC	5 cut(s)	212, 650, 1477, 1873, 2491
SlaI	CTCGAG	2 cut(s)	216, 2453
SmiMI	CAYNNNNRTG	5 cut(s)	1685, 3448, 3683, 3731, 3752
SphI	GCATGC	2 cut(s)	2788, 3202
SsiI	CCGC	5 cut(s)	45, 1230, 1571, 3566, 3625
SspI	AATATT	1 cut(s)	1553
SspMI	CTAG	9 cut(s)	1041, 1052, 1094, 1143, 1481, 3023, 3350, 3656, 3701
SstI	GAGCTC	1 cut(s)	1893
StyD4I	CCNGG	3 cut(s)	443, 646, 1673
StyI	CCWWGG	1 cut(s)	534
TaiI	ACGT	4 cut(s)	1467, 2555, 2672, 3247
TatI	WGTACW	3 cut(s)	346, 428, 2156
TauI	GCSGC	1 cut(s)	1232
TscAI	CASTG	6 cut(s)	294, 982, 1245, 1687, 2989, 3517
TseFI	GTSAC	3 cut(s)	73, 1728, 1837
TseI	GCWGC	8 cut(s)	107, 233, 938, 1636, 1954, 2073, 2415, 2794
Tsp45I	GTSAC	3 cut(s)	73, 1728, 1837
TspGWI	ACGGA	5 cut(s)	258, 792, 803, 1357, 2045
TspRI	CASTG	6 cut(s)	294, 982, 1245, 1687, 2989, 3517
Van91I	CCANNNNNTGG	1 cut(s)	659
Vha464I	CTTAAG	3 cut(s)	1250, 1373, 2150
VpaK11BI	GGWCC	5 cut(s)	212, 650, 1477, 1873, 2491
VspI	ATTAAT	1 cut(s)	3141
XagI	CCTNNNNNAGG	2 cut(s)	219, 1160
XceI	RCATGY	6 cut(s)	1127, 2255, 2788, 2893, 3202, 3635
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2218
XhoI	CTCGAG	2 cut(s)	216, 2453
XmiI	GTMKAC	2 cut(s)	272, 529
XmnI	GAANNNNTTC	2 cut(s)	1797, 2997
XspI	CTAG	9 cut(s)	1041, 1052, 1094, 1143, 1481, 3023, 3350, 3656, 3701
ZrmI	AGTACT	1 cut(s)	430
Zsp2I	ATGCAT	3 cut(s)	3445, 3637, 3743

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.