MD00G1053900.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0017270
Physical Location & Seq
Forward (+)
10057371 .. 10074187
16817 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1053900.v1.1.491

Sequence Viewer

Length: 1203 bp
ATGAAGACCATGCGTGAGATTCTGCGTGCCGATTCTTCTGATGATTTCGATATGCAGATGATAGGCAAGTTCTTGAGTTTCGCTTCCAGAGGCGACAGGGTTGGACTGAACCAGATGTTGATCCAGGGCATATCTCCTGATGTCCAAGACTACGACAACAGGACCGCGCTCCATCTCGCTGCGAGCGAAGGTCACACTCCCATTGTTGAGCTTCTTGTGTGTTACAAGGCCAATGTTAATCTCAAAGATCGATGGAAACGGACTCCCCTGACAGATGCGAGACTCTATGGACATCGAGATATATGCAGGATTCTTGAAGTAAATGGTGGCAATGACTTTACCAATGATCAACTAATGACTGTTCGGCATGAACAAGATTCAAATGACGTGAAGTTTGACATGTCAGAACTAAACACAGAGCATTCATCAACAATTCGGCAGGGTGCATTTGGTGAATCTGAAAAGGTCAAATGGCGTGGAACCTGGGTCGTTAAAACTGTTGTCAAGAGGCACATATGCAATCCTGTAAAAATGATCCTCTCTGCTAAGGATAACACTCTCCTGCGAGAACTTAGACATCCCAATATTTTGCAATTTCTTGGTTCGATTGTGCAACAGGAAGAGATGGTTCTGATTACTGAGTATCTACCAAAAGGTAACCTGGAAAATATTTTGAGGCAAAAAGCTCGACTTGATTTGCCTACTGCTTTTCGCTATGCACTTGATATTGCTAGGGGAATGAACTATCTTCACGAACACAAGCCCTGCCCAATAGTTCACAACCATTTGGGTACCAGAAACTTGTTGCAGGACGAAGGTGGACACTTGAAGATCGGCGAGTATTGGGTTCAAATGTTGTACGAGCAAATTAACCCAGATCAAAATGGTTGGAAAAGAAGCAATCCCCGTAACATCGAAAACCTAATAGATGACCCTAAGAAAGATGTTCGTACGTTTGGTTTCATATTTTATCAGATGCTAGAAGGAAGGCACTTCATGACCAGCACGAACTTTGACACCATAAATGAGGAACCTGTTGATTTTAAGCCGAAATTCCACTTAAGTCGATGTACAAACAGAATTCATCAGTTGATAGAGCATTGTACAAGTAATAATCCTTCGGAAATACCCTCATTTGGTGCTGTCATCGAGATTCTGGAAGAGGAATCGTCATCTTTAGGGAGAGCTGTATGCCCTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
ko03320 ko04360 ko04510 ko05100 ko05213 map03320 map04360 map04510 map05100 map05213
Pfam Domains
Protein Families
Ank_2 Ank_4 Ank_5 Pkinase_Tyr

Protein Analysis

401

Amino Acids

46.15

Weight (kDa)

6.36

Isoelectric Point (pI)

47.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 36 - 105 9.8e-13 Ankyrin repeats (3 copies)
Ank_5 PF13857 43 - 90 1.9e-06 Ankyrin repeats (many copies)
Ank PF00023 52 - 84 1.7e-08 Ankyrin repeat
Ank_4 PF13637 57 - 105 9.5e-06 Ankyrin repeats (many copies)
PK_Tyr_Ser-Thr PF07714 142 - 385 1.6e-27 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 143 - 379 3.3e-21 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017270)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g27960
malus_domestica MD00G1053900.v1.1
prunus_persica Prupe.3G150700_v2.0.a1
pyrus_communis pycom17g25570
rosa_chinensis RchiOBHm_Chr5g0011771
rosa_laevigata RLG00000031861
rosa_roxburghii Rroxscaffold_1G00064780
rosa_rugosa Rorug04G0458900
rosa_samantha Rh5AG090600 Rh5BG086100 Rh5CG099900 Rh5DG086300
rosa_wichuraiana Rw5G007910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 791
AccB1I GGYRCC 1 cut(s) 791
AccII CGCG 1 cut(s) 167
AciI CCGC 1 cut(s) 165
AclWI GGATC 2 cut(s) 115, 529
AcsI RAATTY 2 cut(s) 1052, 1080
AfaI GTAC 5 cut(s) 793, 860, 952, 1072, 1105
AfiI CCNNNNNNNGG 1 cut(s) 1136
AflII CTTAAG 1 cut(s) 1060
AflIII ACRYGT 1 cut(s) 399
AgsI TTSAA 4 cut(s) 317, 381, 829, 851
AjiI CACGTC 1 cut(s) 388
AjnI CCWGG 3 cut(s) 123, 482, 660
AluBI AGCT 3 cut(s) 211, 686, 1187
AluI AGCT 3 cut(s) 211, 686, 1187
Alw26I GTCTC 1 cut(s) 274
AlwI GGATC 2 cut(s) 115, 529
AoxI GGCC 1 cut(s) 228
ApeKI GCWGC 1 cut(s) 179
ApoI RAATTY 2 cut(s) 1052, 1080
ArsI GACNNNNNNTTYG 2 cut(s) 377, 409
Asp718I GGTACC 1 cut(s) 791
AspLEI GCGC 1 cut(s) 169
AspS9I GGNCC 1 cut(s) 162
AsuHPI GGTGA 1 cut(s) 464
AvaII GGWCC 1 cut(s) 162
BanI GGYRCC 1 cut(s) 791
BarI GAAGNNNNNNTAC 2 cut(s) 1102, 1134
BbsI GAAGAC 1 cut(s) 11
BbvI GCAGC 1 cut(s) 166
BccI CCATC 3 cut(s) 180, 246, 619
BcgI CGANNNNNNTGC 4 cut(s) 285, 319, 668, 702
BciT130I CCWGG 3 cut(s) 125, 484, 662
BclI TGATCA 1 cut(s) 346
BcoDI GTCTC 1 cut(s) 274
BfaI CTAG 2 cut(s) 732, 980
BfrI CTTAAG 1 cut(s) 1060
BisI GCNGC 1 cut(s) 180
BlsI GCNGC 1 cut(s) 181
Bme1390I CCNGG 3 cut(s) 125, 484, 662
Bme18I GGWCC 1 cut(s) 162
BmgBI CACGTC 1 cut(s) 388
BmgT120I GGNCC 1 cut(s) 162
BmiI GGNNCC 3 cut(s) 481, 793, 1032
BmrFI CCNGG 3 cut(s) 125, 484, 662
BmsI GCATC 2 cut(s) 265, 966
BpiI GAAGAC 1 cut(s) 11
Bpu10I CCTNAGC 1 cut(s) 546
BpuEI CTTGAG 1 cut(s) 94
Bsa29I ATCGAT 1 cut(s) 250
BsaBI GATNNNNATC 1 cut(s) 119
BsaJI CCNNGG 2 cut(s) 124, 483
Bsc4I CCNNNNNNNGG 1 cut(s) 1136
Bse3DI GCAATG 1 cut(s) 337
Bse8I GATNNNNATC 1 cut(s) 119
BseBI CCWGG 3 cut(s) 125, 484, 662
BseCI ATCGAT 1 cut(s) 250
BseDI CCNNGG 2 cut(s) 124, 483
BseGI GGATG 1 cut(s) 577
BseJI GATNNNNATC 1 cut(s) 119
BseLI CCNNNNNNNGG 1 cut(s) 1136
BseMI GCAATG 1 cut(s) 337
BseMII CTCAG 1 cut(s) 630
BseXI GCAGC 1 cut(s) 166
Bsh1236I CGCG 1 cut(s) 167
BshFI GGCC 1 cut(s) 230
BshNI GGYRCC 1 cut(s) 791
BshVI ATCGAT 1 cut(s) 250
BsiWI CGTACG 1 cut(s) 950
BslI CCNNNNNNNGG 1 cut(s) 1136
BsmAI GTCTC 1 cut(s) 274
BsmI GAATGC 1 cut(s) 421
BsnI GGCC 1 cut(s) 230
Bsp1407I TGTACA 2 cut(s) 1070, 1103
Bsp143I GATC 6 cut(s) 120, 247, 346, 534, 831, 877
BspACI CCGC 1 cut(s) 165
BspANI GGCC 1 cut(s) 230
BspCNI CTCAG 1 cut(s) 631
BspDI ATCGAT 1 cut(s) 250
BspFNI CGCG 1 cut(s) 167
BspHI TCATGA 1 cut(s) 996
BspLI GGNNCC 3 cut(s) 481, 793, 1032
BspPI GGATC 2 cut(s) 115, 529
BspT107I GGYRCC 1 cut(s) 791
BspTI CTTAAG 1 cut(s) 1060
BsrDI GCAATG 1 cut(s) 337
BsrGI TGTACA 2 cut(s) 1070, 1103
BssECI CCNNGG 2 cut(s) 124, 483
BssMI GATC 6 cut(s) 120, 247, 346, 534, 831, 877
Bst2UI CCWGG 3 cut(s) 125, 484, 662
Bst4CI ACNGT 2 cut(s) 361, 499
Bst6I CTCTTC 2 cut(s) 615, 1155
BstAFI CTTAAG 1 cut(s) 1060
BstAUI TGTACA 2 cut(s) 1070, 1103
BstC8I GCNNGC 2 cut(s) 27, 184
BstDEI CTNAG 4 cut(s) 546, 572, 639, 936
BstEII GGTNACC 1 cut(s) 656
BstF5I GGATG 1 cut(s) 577
BstFNI CGCG 1 cut(s) 167
BstHHI GCGC 1 cut(s) 169
BstKTI GATC 6 cut(s) 123, 250, 349, 537, 834, 880
BstMAI GTCTC 1 cut(s) 274
BstMBI GATC 6 cut(s) 120, 247, 346, 534, 831, 877
BstNI CCWGG 3 cut(s) 125, 484, 662
BstNSI RCATGY 1 cut(s) 403
BstPI GGTNACC 1 cut(s) 656
BstSCI CCNGG 3 cut(s) 123, 482, 660
BstUI CGCG 1 cut(s) 167
BstV1I GCAGC 1 cut(s) 166
BstV2I GAAGAC 1 cut(s) 11
Bsu15I ATCGAT 1 cut(s) 250
BsuRI GGCC 1 cut(s) 230
BsuTUI ATCGAT 1 cut(s) 250
BtrI CACGTC 1 cut(s) 388
BtsCI GGATG 1 cut(s) 577
Cac8I GCNNGC 2 cut(s) 27, 184
CciI TCATGA 1 cut(s) 996
CfoI GCGC 1 cut(s) 169
Cfr13I GGNCC 1 cut(s) 162
ClaI ATCGAT 1 cut(s) 250
Csp6I GTAC 5 cut(s) 792, 859, 951, 1071, 1104
CspCI CAANNNNNGTGG 2 cut(s) 457, 492
CviAII CATG 4 cut(s) 10, 368, 400, 997
CviJI RGCY 6 cut(s) 211, 230, 686, 763, 1048, 1187
CviKI_1 RGCY 6 cut(s) 211, 230, 686, 763, 1048, 1187
CviQI GTAC 5 cut(s) 792, 859, 951, 1071, 1104
DdeI CTNAG 4 cut(s) 546, 572, 639, 936
DpnI GATC 6 cut(s) 122, 249, 348, 536, 833, 879
DpnII GATC 6 cut(s) 120, 247, 346, 534, 831, 877
Eam1104I CTCTTC 2 cut(s) 615, 1155
EarI CTCTTC 2 cut(s) 615, 1155
Eco47I GGWCC 1 cut(s) 162
Eco91I GGTNACC 1 cut(s) 656
EcoO65I GGTNACC 1 cut(s) 656
EcoRI GAATTC 1 cut(s) 1080
EcoRII CCWGG 3 cut(s) 123, 482, 660
FaeI CATG 4 cut(s) 13, 371, 403, 1000
FalI AAGNNNNNCTT 2 cut(s) 675, 707
FatI CATG 4 cut(s) 9, 367, 399, 996
FauNDI CATATG 1 cut(s) 515
FbaI TGATCA 1 cut(s) 346
Fnu4HI GCNGC 1 cut(s) 180
FokI GGATG 1 cut(s) 564
Fsp4HI GCNGC 1 cut(s) 180
FspBI CTAG 2 cut(s) 732, 980
GlaI GCGC 1 cut(s) 168
GluI GCNGC 1 cut(s) 180
HaeIII GGCC 1 cut(s) 230
HhaI GCGC 1 cut(s) 169
Hin1II CATG 4 cut(s) 13, 371, 403, 1000
Hin6I GCGC 1 cut(s) 167
HinP1I GCGC 1 cut(s) 167
HinfI GANTC 9 cut(s) 19, 32, 262, 282, 310, 377, 455, 1153, 1166
HphI GGTGA 1 cut(s) 464
Hpy166II GTNNAC 2 cut(s) 778, 821
Hpy188I TCNGA 6 cut(s) 40, 406, 460, 633, 975, 1123
Hpy8I GTNNAC 2 cut(s) 778, 821
HpyAV CCTTC 5 cut(s) 182, 809, 977, 981, 1128
HpyCH4III ACNGT 2 cut(s) 361, 499
HpyCH4IV ACGT 2 cut(s) 387, 953
HpyCH4V TGCA 8 cut(s) 55, 306, 446, 519, 592, 613, 719, 808
HpyF3I CTNAG 4 cut(s) 546, 572, 639, 936
HpySE526I ACGT 2 cut(s) 387, 953
Hsp92II CATG 4 cut(s) 13, 371, 403, 1000
HspAI GCGC 1 cut(s) 167
KpnI GGTACC 1 cut(s) 795
Ksp22I TGATCA 1 cut(s) 346
Kzo9I GATC 6 cut(s) 120, 247, 346, 534, 831, 877
LmnI GCTCC 1 cut(s) 174
Lsp1109I GCAGC 1 cut(s) 166
LweI GCATC 2 cut(s) 265, 966
MaeI CTAG 2 cut(s) 732, 980
MaeII ACGT 2 cut(s) 387, 953
MaeIII GTNAC 4 cut(s) 191, 221, 656, 908
MalI GATC 6 cut(s) 122, 249, 348, 536, 833, 879
MboI GATC 6 cut(s) 120, 247, 346, 534, 831, 877
MboII GAAGA 6 cut(s) 16, 27, 632, 740, 841, 1172
MluCI AATT 5 cut(s) 432, 593, 867, 1052, 1080
MlyI GAGTC 2 cut(s) 256, 276
MmeI TCCRAC 2 cut(s) 82, 869
MnlI CCTC 7 cut(s) 83, 501, 548, 669, 1021, 1141, 1156
MseI TTAA 5 cut(s) 237, 492, 870, 1044, 1061
MspCI CTTAAG 1 cut(s) 1060
MspR9I CCNGG 3 cut(s) 125, 484, 662
Mva1269I GAATGC 1 cut(s) 421
MvaI CCWGG 3 cut(s) 125, 484, 662
MvnI CGCG 1 cut(s) 167
NdeI CATATG 1 cut(s) 515
NdeII GATC 6 cut(s) 120, 247, 346, 534, 831, 877
NlaIII CATG 4 cut(s) 13, 371, 403, 1000
NlaIV GGNNCC 3 cut(s) 481, 793, 1032
NmuCI GTSAC 1 cut(s) 191
NspI RCATGY 1 cut(s) 403
PagI TCATGA 1 cut(s) 996
PciI ACATGT 1 cut(s) 399
PcsI WCGNNNNNNNCGW 1 cut(s) 183
PctI GAATGC 1 cut(s) 421
PfeI GAWTC 7 cut(s) 19, 32, 310, 377, 455, 1153, 1166
Pfl23II CGTACG 1 cut(s) 950
PkrI GCNGC 1 cut(s) 181
PleI GAGTC 2 cut(s) 256, 276
PpsI GAGTC 2 cut(s) 256, 276
PscI ACATGT 1 cut(s) 399
Psp6I CCWGG 3 cut(s) 123, 482, 660
PspEI GGTNACC 1 cut(s) 656
PspGI CCWGG 3 cut(s) 123, 482, 660
PspLI CGTACG 1 cut(s) 950
PspN4I GGNNCC 3 cut(s) 481, 793, 1032
PspPI GGNCC 1 cut(s) 162
RsaI GTAC 5 cut(s) 793, 860, 952, 1072, 1105
RsaNI GTAC 5 cut(s) 792, 859, 951, 1071, 1104
SaqAI TTAA 5 cut(s) 237, 492, 870, 1044, 1061
SatI GCNGC 1 cut(s) 180
Sau3AI GATC 6 cut(s) 120, 247, 346, 534, 831, 877
Sau96I GGNCC 1 cut(s) 162
SchI GAGTC 2 cut(s) 256, 276
ScrFI CCNGG 3 cut(s) 125, 484, 662
SfaNI GCATC 2 cut(s) 265, 966
SinI GGWCC 1 cut(s) 162
SmlI CTYRAG 2 cut(s) 73, 1060
SmoI CTYRAG 2 cut(s) 73, 1060
Sse9I AATT 5 cut(s) 432, 593, 867, 1052, 1080
SsiI CCGC 1 cut(s) 165
SspI AATATT 2 cut(s) 586, 670
SspMI CTAG 2 cut(s) 732, 980
StyD4I CCNGG 3 cut(s) 123, 482, 660
TaaI ACNGT 2 cut(s) 361, 499
TaiI ACGT 2 cut(s) 390, 956
TaqI TCGA 8 cut(s) 48, 250, 295, 605, 688, 915, 1066, 1149
TasI AATT 5 cut(s) 432, 593, 867, 1052, 1080
TatI WGTACW 2 cut(s) 1070, 1103
TfiI GAWTC 7 cut(s) 19, 32, 310, 377, 455, 1153, 1166
Tru1I TTAA 5 cut(s) 237, 492, 870, 1044, 1061
Tru9I TTAA 5 cut(s) 237, 492, 870, 1044, 1061
TseFI GTSAC 1 cut(s) 191
TseI GCWGC 1 cut(s) 179
Tsp45I GTSAC 1 cut(s) 191
TspDTI ATGAA 7 cut(s) 17, 384, 414, 755, 952, 985, 1073
TspGWI ACGGA 1 cut(s) 274
Vha464I CTTAAG 1 cut(s) 1060
VpaK11BI GGWCC 1 cut(s) 162
XapI RAATTY 2 cut(s) 1052, 1080
XceI RCATGY 1 cut(s) 403
XspI CTAG 2 cut(s) 732, 980
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.