Rroxscaffold_1G00064780

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0017270
Physical Location & Seq
Reverse (-)
86591424 .. 86594663
3240 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00064780.1

Sequence Viewer

Length: 1212 bp
ATGAAGACGATGGGTGAGATTCTCCATGCCCGCGATGACTTCGACATGCAACTGATTGGGAAGTTCTTGAGCTTCGCTTCGAGAGGCGACCGAGTTGGACTGAACCAGATGTTGATCCAAGGCATATCTCCTGATGTGCAAGACTACGATAACAGGACGGCGCTTCATCTGGCGGCGAGTGAGGGTCACACTCCCATTGTTGAGCTTCTCGTGTGCTACAAGGCCAACGTTAATCTCCAAGACCGATGGAAAAGAACTCCCCTGACAGATGCAAGACTCTATGGACATCGAGATATATGCAGGATTCTTGAAGTGAATGGAGCCAATGACTTTACCAGTGATCAGTTGATGACCGTTCGACACGAACAAGATTCAAATGATGTGAAGTTTGACATGACGGAATTAGTTACAGATAATTCATCAGCGATTCAACAGGGTTCATTTGGTGAATCTGAAAAGGTGAAGTGGCGTGGAATCTGGGTCGTAAAGACCGTTGTCAAAAAAGACATATGTGCTCCTGTTAGGCCCGACCCTAGCACTGGGCATGCTTGGCGACAGCCCAGGGCCGCAGAGCAAATGGGGCACCAAATGATACTCTCTGCTAAGGATAATACTCTCTTACGGGAGCTTAGACATCCCAATATATTGCAGTTTCTTGGTTCAATTGTGCAGCAAGAGGAGATGGTTCTAATTACCGAGTATCTACCAAAAGGCAATCTCGAGGATATTTTGACGCAAAGAACACGTCTTGATGTGCCCACGGCTCTACGCTATGCACTTGATATTGCTAGAAACTTGTTTCAGGATGAAGGTGGGCACTTGAAGATTGGGGAGTATTGGGTTCAAATGTTGCATGAACAAATTAACCCAGATCAAAATGGAAGGCAAAACCGTGATGGTACACAGGATTTAACGAAGATAGATATTTGGAGGTTCGGATTCATATATTATCAGATGCTAGAAGGAATGCACTTCCAGACCAACACAAACTCTGATCAACCCGAACTCGTTGATTTTAAACCGAAATTCCATTTAAGTCGATGTTCTAATAGAATTCAAGAGTTGATAGAGAAATGCACAGGTCATCCTTCTCGAAGACCCCAATTCGAAGAAGTCATACCTATCCTAGAAGAGGAACTATGGTCTGTGGGTAGAGGTGTATGTCCAGTTTACTGCGTACAGATGGAGATGGGCAACAAGTGCACCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
ko03320 ko04360 ko04510 ko05100 ko05213 map03320 map04360 map04510 map05100 map05213
Pfam Domains
Protein Families
Ank_2 Ank_4 Ank_5 Pkinase_Tyr

Protein Analysis

403

Amino Acids

46.42

Weight (kDa)

5.84

Isoelectric Point (pI)

39.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 34 - 104 7.5e-13 Ankyrin repeats (3 copies)
Ank_5 PF13857 41 - 88 2.6e-06 Ankyrin repeats (many copies)
Ank PF00023 50 - 82 1.8e-08 Ankyrin repeat
Ank_4 PF13637 55 - 103 2.3e-06 Ankyrin repeats (many copies)
PK_Tyr_Ser-Thr PF07714 202 - 264 3.3e-10 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 203 - 264 2.4e-06 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017270)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g27960
malus_domestica MD00G1053900.v1.1
prunus_persica Prupe.3G150700_v2.0.a1
pyrus_communis pycom17g25570
rosa_chinensis RchiOBHm_Chr5g0011771
rosa_laevigata RLG00000031861
rosa_roxburghii Rroxscaffold_1G00064780
rosa_rugosa Rorug04G0458900
rosa_samantha Rh5AG090600 Rh5BG086100 Rh5CG099900 Rh5DG086300
rosa_wichuraiana Rw5G007910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 582
AccII CGCG 1 cut(s) 33
AciI CCGC 3 cut(s) 31, 173, 567
AclI AACGTT 1 cut(s) 228
AclWI GGATC 1 cut(s) 109
AcsI RAATTY 2 cut(s) 1025, 1053
AfaI GTAC 2 cut(s) 901, 1179
AfiI CCNNNNNNNGG 2 cut(s) 539, 1132
AflIII ACRYGT 1 cut(s) 743
AgsI TTSAA 7 cut(s) 311, 375, 431, 663, 823, 845, 1058
AjiI CACGTC 1 cut(s) 746
AjnI CCWGG 1 cut(s) 560
AluBI AGCT 3 cut(s) 72, 205, 628
AluI AGCT 3 cut(s) 72, 205, 628
Alw21I GWGCWC 2 cut(s) 517, 1205
Alw44I GTGCAC 1 cut(s) 1201
AlwI GGATC 1 cut(s) 109
Ama87I CYCGRG 1 cut(s) 719
AoxI GGCC 3 cut(s) 222, 524, 564
ApaLI GTGCAC 1 cut(s) 1201
ApeKI GCWGC 1 cut(s) 670
ApoI RAATTY 2 cut(s) 1025, 1053
ArsI GACNNNNNNTTYG 2 cut(s) 730, 762
AspLEI GCGC 1 cut(s) 163
AspS9I GGNCC 2 cut(s) 525, 564
AsuHPI GGTGA 3 cut(s) 26, 458, 472
AsuII TTCGAA 1 cut(s) 1107
AvaI CYCGRG 1 cut(s) 719
BaeGI GKGCMC 4 cut(s) 585, 759, 819, 1205
BanI GGYRCC 1 cut(s) 582
BarI GAAGNNNNNNTAC 2 cut(s) 1101, 1133
BauI CACGAG 1 cut(s) 209
BbsI GAAGAC 2 cut(s) 11, 1102
Bbv12I GWGCWC 2 cut(s) 517, 1205
BbvI GCAGC 1 cut(s) 682
BccI CCATC 6 cut(s) 4, 240, 676, 890, 1177, 1183
BceAI ACGGC 2 cut(s) 174, 777
BcgI CGANNNNNNTGC 2 cut(s) 279, 313
BciT130I CCWGG 1 cut(s) 562
BclI TGATCA 2 cut(s) 340, 994
BfaI CTAG 4 cut(s) 534, 789, 959, 1127
BfoI RGCGCY 1 cut(s) 164
BisI GCNGC 3 cut(s) 174, 567, 671
BlsI GCNGC 3 cut(s) 175, 568, 672
Bme1390I CCNGG 1 cut(s) 562
BmeT110I CYCGRG 1 cut(s) 719
BmgBI CACGTC 1 cut(s) 746
BmgT120I GGNCC 2 cut(s) 525, 564
BmiI GGNNCC 2 cut(s) 322, 584
BmrFI CCNGG 1 cut(s) 562
BmrI ACTGGG 1 cut(s) 549
BmsI GCATC 2 cut(s) 259, 945
BmuI ACTGGG 1 cut(s) 549
BoxI GACNNNNGTC 1 cut(s) 494
BpiI GAAGAC 2 cut(s) 11, 1102
Bpu10I CCTNAGC 1 cut(s) 603
Bpu14I TTCGAA 1 cut(s) 1107
BpuEI CTTGAG 1 cut(s) 88
BsaBI GATNNNNATC 1 cut(s) 113
BsaJI CCNNGG 4 cut(s) 118, 560, 561, 759
Bsc4I CCNNNNNNNGG 2 cut(s) 539, 1132
Bse1I ACTGG 3 cut(s) 336, 544, 1166
Bse8I GATNNNNATC 1 cut(s) 113
BseBI CCWGG 1 cut(s) 562
BseDI CCNNGG 4 cut(s) 118, 560, 561, 759
BseGI GGATG 3 cut(s) 634, 811, 1084
BseJI GATNNNNATC 1 cut(s) 113
BseLI CCNNNNNNNGG 2 cut(s) 539, 1132
BseNI ACTGG 3 cut(s) 336, 544, 1166
BseRI GAGGAG 1 cut(s) 692
BseSI GKGCMC 4 cut(s) 585, 759, 819, 1205
BseXI GCAGC 1 cut(s) 682
BsgI GTGCAG 1 cut(s) 689
Bsh1236I CGCG 1 cut(s) 33
Bsh1285I CGRYCG 1 cut(s) 91
BshFI GGCC 3 cut(s) 224, 526, 566
BshNI GGYRCC 1 cut(s) 582
BsiEI CGRYCG 1 cut(s) 91
BsiHKAI GWGCWC 2 cut(s) 517, 1205
BsiHKCI CYCGRG 1 cut(s) 719
BslI CCNNNNNNNGG 2 cut(s) 539, 1132
BsmI GAATGC 1 cut(s) 972
BsnI GGCC 3 cut(s) 224, 526, 566
BsoBI CYCGRG 1 cut(s) 719
Bsp119I TTCGAA 1 cut(s) 1107
Bsp1286I GDGCHC 5 cut(s) 517, 585, 759, 819, 1205
Bsp143I GATC 4 cut(s) 114, 340, 871, 994
BspACI CCGC 3 cut(s) 31, 173, 567
BspANI GGCC 3 cut(s) 224, 526, 566
BspFNI CGCG 1 cut(s) 33
BspLI GGNNCC 2 cut(s) 322, 584
BspPI GGATC 1 cut(s) 109
BspT104I TTCGAA 1 cut(s) 1107
BspT107I GGYRCC 1 cut(s) 582
BsrI ACTGG 3 cut(s) 336, 544, 1166
BssECI CCNNGG 4 cut(s) 118, 560, 561, 759
BssMI GATC 4 cut(s) 114, 340, 871, 994
BssSI CACGAG 1 cut(s) 209
BssT1I CCWWGG 1 cut(s) 118
Bst2BI CACGAG 1 cut(s) 209
Bst2UI CCWGG 1 cut(s) 562
Bst4CI ACNGT 3 cut(s) 355, 493, 893
Bst6I CTCTTC 1 cut(s) 1125
BstAPI GCANNNNNTGC 1 cut(s) 1200
BstBI TTCGAA 1 cut(s) 1107
BstC8I GCNNGC 2 cut(s) 31, 546
BstDEI CTNAG 2 cut(s) 603, 629
BstDSI CCRYGG 1 cut(s) 759
BstENI CCTNNNNNAGG 1 cut(s) 1130
BstF5I GGATG 3 cut(s) 634, 811, 1084
BstFNI CGCG 1 cut(s) 33
BstH2I RGCGCY 1 cut(s) 164
BstHHI GCGC 1 cut(s) 163
BstKTI GATC 4 cut(s) 117, 343, 874, 997
BstMBI GATC 4 cut(s) 114, 340, 871, 994
BstMCI CGRYCG 1 cut(s) 91
BstMWI GCNNNNNNNGC 3 cut(s) 550, 580, 1200
BstNI CCWGG 1 cut(s) 562
BstNSI RCATGY 2 cut(s) 49, 548
BstPAI GACNNNNGTC 1 cut(s) 494
BstSCI CCNGG 1 cut(s) 560
BstSLI GKGCMC 4 cut(s) 585, 759, 819, 1205
BstUI CGCG 1 cut(s) 33
BstV1I GCAGC 1 cut(s) 682
BstV2I GAAGAC 2 cut(s) 11, 1102
BsuRI GGCC 3 cut(s) 224, 526, 566
BtgI CCRYGG 1 cut(s) 759
BtgZI GCGATG 1 cut(s) 48
BtrI CACGTC 1 cut(s) 746
BtsCI GGATG 3 cut(s) 634, 811, 1084
BtsIMutI CAGTG 2 cut(s) 343, 537
Cac8I GCNNGC 2 cut(s) 31, 546
CfoI GCGC 1 cut(s) 163
Cfr13I GGNCC 2 cut(s) 525, 564
CseI GACGC 1 cut(s) 742
Csp6I GTAC 2 cut(s) 900, 1178
CviAII CATG 5 cut(s) 26, 46, 394, 545, 854
CviJI RGCY 9 cut(s) 72, 205, 224, 323, 526, 559, 566, 628, 764
CviKI_1 RGCY 9 cut(s) 72, 205, 224, 323, 526, 559, 566, 628, 764
CviQI GTAC 2 cut(s) 900, 1178
DdeI CTNAG 2 cut(s) 603, 629
DpnI GATC 4 cut(s) 116, 342, 873, 996
DpnII GATC 4 cut(s) 114, 340, 871, 994
DraI TTTAAA 1 cut(s) 1018
Eam1104I CTCTTC 1 cut(s) 1125
EarI CTCTTC 1 cut(s) 1125
Eco130I CCWWGG 1 cut(s) 118
Eco88I CYCGRG 1 cut(s) 719
EcoNI CCTNNNNNAGG 1 cut(s) 1130
EcoRI GAATTC 1 cut(s) 1053
EcoRII CCWGG 1 cut(s) 560
EcoT14I CCWWGG 1 cut(s) 118
ErhI CCWWGG 1 cut(s) 118
FaeI CATG 5 cut(s) 29, 49, 397, 548, 857
FatI CATG 5 cut(s) 25, 45, 393, 544, 853
FauI CCCGC 1 cut(s) 38
FauNDI CATATG 1 cut(s) 509
FbaI TGATCA 2 cut(s) 340, 994
Fnu4HI GCNGC 3 cut(s) 174, 567, 671
FokI GGATG 3 cut(s) 621, 818, 1071
Fsp4HI GCNGC 3 cut(s) 174, 567, 671
FspBI CTAG 4 cut(s) 534, 789, 959, 1127
GlaI GCGC 1 cut(s) 162
GluI GCNGC 3 cut(s) 174, 567, 671
HaeII RGCGCY 1 cut(s) 164
HaeIII GGCC 3 cut(s) 224, 526, 566
HgaI GACGC 1 cut(s) 742
HhaI GCGC 1 cut(s) 163
Hin1II CATG 5 cut(s) 29, 49, 397, 548, 857
Hin6I GCGC 1 cut(s) 161
HinP1I GCGC 1 cut(s) 161
HinfI GANTC 8 cut(s) 19, 276, 304, 371, 427, 449, 474, 939
HphI GGTGA 3 cut(s) 26, 458, 472
Hpy166II GTNNAC 3 cut(s) 902, 1171, 1203
Hpy188I TCNGA 4 cut(s) 454, 938, 954, 994
Hpy8I GTNNAC 3 cut(s) 902, 1171, 1203
HpyAV CCTTC 4 cut(s) 803, 876, 956, 1098
HpyCH4III ACNGT 3 cut(s) 355, 493, 893
HpyCH4IV ACGT 2 cut(s) 228, 745
HpyF10VI GCNNNNNNNGC 3 cut(s) 550, 580, 1200
HpyF3I CTNAG 2 cut(s) 603, 629
HpySE526I ACGT 2 cut(s) 228, 745
Hsp92II CATG 5 cut(s) 29, 49, 397, 548, 857
HspAI GCGC 1 cut(s) 161
Ksp22I TGATCA 2 cut(s) 340, 994
Kzo9I GATC 4 cut(s) 114, 340, 871, 994
LmnI GCTCC 3 cut(s) 320, 520, 625
Lsp1109I GCAGC 1 cut(s) 682
LweI GCATC 2 cut(s) 259, 945
MaeI CTAG 4 cut(s) 534, 789, 959, 1127
MaeII ACGT 2 cut(s) 228, 745
MaeIII GTNAC 2 cut(s) 185, 406
MalI GATC 4 cut(s) 116, 342, 873, 996
MboI GATC 4 cut(s) 114, 340, 871, 994
MboII GAAGA 6 cut(s) 16, 835, 928, 1107, 1121, 1142
MfeI CAATTG 1 cut(s) 663
MhlI GDGCHC 5 cut(s) 517, 585, 759, 819, 1205
MluCI AATT 8 cut(s) 401, 415, 663, 690, 861, 1025, 1053, 1103
MlyI GAGTC 1 cut(s) 270
MmeI TCCRAC 1 cut(s) 76
MnlI CCTC 7 cut(s) 77, 175, 670, 715, 924, 1126, 1148
MseI TTAA 5 cut(s) 231, 864, 911, 1017, 1034
MspR9I CCNGG 1 cut(s) 562
MunI CAATTG 1 cut(s) 663
Mva1269I GAATGC 1 cut(s) 972
MvaI CCWGG 1 cut(s) 562
MvnI CGCG 1 cut(s) 33
MwoI GCNNNNNNNGC 3 cut(s) 550, 580, 1200
NdeI CATATG 1 cut(s) 509
NdeII GATC 4 cut(s) 114, 340, 871, 994
NlaIII CATG 5 cut(s) 29, 49, 397, 548, 857
NlaIV GGNNCC 2 cut(s) 322, 584
NmuCI GTSAC 1 cut(s) 185
NspI RCATGY 2 cut(s) 49, 548
NspV TTCGAA 1 cut(s) 1107
PaeI GCATGC 1 cut(s) 548
PaeR7I CTCGAG 1 cut(s) 719
PasI CCCWGGG 1 cut(s) 561
PcsI WCGNNNNNNNCGW 1 cut(s) 489
PctI GAATGC 1 cut(s) 972
PfeI GAWTC 7 cut(s) 19, 304, 371, 427, 449, 474, 939
PkrI GCNGC 3 cut(s) 175, 568, 672
PleI GAGTC 1 cut(s) 270
PpsI GAGTC 1 cut(s) 270
PshAI GACNNNNGTC 1 cut(s) 494
Psp1406I AACGTT 1 cut(s) 228
Psp6I CCWGG 1 cut(s) 560
PspGI CCWGG 1 cut(s) 560
PspN4I GGNNCC 2 cut(s) 322, 584
PspPI GGNCC 2 cut(s) 525, 564
RsaI GTAC 2 cut(s) 901, 1179
RsaNI GTAC 2 cut(s) 900, 1178
SaqAI TTAA 5 cut(s) 231, 864, 911, 1017, 1034
SatI GCNGC 3 cut(s) 174, 567, 671
Sau3AI GATC 4 cut(s) 114, 340, 871, 994
Sau96I GGNCC 2 cut(s) 525, 564
SchI GAGTC 1 cut(s) 270
ScrFI CCNGG 1 cut(s) 562
SduI GDGCHC 5 cut(s) 517, 585, 759, 819, 1205
SfaNI GCATC 2 cut(s) 259, 945
Sfr274I CTCGAG 1 cut(s) 719
SfuI TTCGAA 1 cut(s) 1107
SlaI CTCGAG 1 cut(s) 719
SmlI CTYRAG 2 cut(s) 67, 719
SmoI CTYRAG 2 cut(s) 67, 719
SphI GCATGC 1 cut(s) 548
Sse9I AATT 8 cut(s) 401, 415, 663, 690, 861, 1025, 1053, 1103
SsiI CCGC 3 cut(s) 31, 173, 567
SspMI CTAG 4 cut(s) 534, 789, 959, 1127
StyD4I CCNGG 1 cut(s) 560
StyI CCWWGG 1 cut(s) 118
TaaI ACNGT 3 cut(s) 355, 493, 893
TaiI ACGT 2 cut(s) 231, 748
TaqI TCGA 8 cut(s) 42, 80, 289, 358, 720, 1039, 1093, 1107
TaqII GACCGA 2 cut(s) 105, 258
TasI AATT 8 cut(s) 401, 415, 663, 690, 861, 1025, 1053, 1103
TauI GCSGC 2 cut(s) 176, 569
TfiI GAWTC 7 cut(s) 19, 304, 371, 427, 449, 474, 939
Tru1I TTAA 5 cut(s) 231, 864, 911, 1017, 1034
Tru9I TTAA 5 cut(s) 231, 864, 911, 1017, 1034
TscAI CASTG 2 cut(s) 343, 544
TseFI GTSAC 1 cut(s) 185
TseI GCWGC 1 cut(s) 670
Tsp45I GTSAC 1 cut(s) 185
TspDTI ATGAA 7 cut(s) 17, 155, 408, 429, 822, 870, 931
TspGWI ACGGA 1 cut(s) 413
TspRI CASTG 2 cut(s) 343, 544
VneI GTGCAC 1 cut(s) 1201
XagI CCTNNNNNAGG 1 cut(s) 1130
XapI RAATTY 2 cut(s) 1025, 1053
XceI RCATGY 2 cut(s) 49, 548
XhoI CTCGAG 1 cut(s) 719
XspI CTAG 4 cut(s) 534, 789, 959, 1127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.