RLG00000031861

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0017270
Physical Location & Seq
Forward (+)
8820943 .. 8824888
3946 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031861

Sequence Viewer

Length: 1245 bp
ATGGGTGAGATTCTCCATGCCCGCGACGACTTCGACATGCAACTGATTGGGAAGTTCTTGAGCTTCGCTTCGAGAGGCGACCGAGTTGGACTGAACCAGATGTTGATCCAAGGCATATCTCCTGATGTGCAAGACTACGATAATAGGACGGCGCTGCATCTGGCGGCGAGTGAGGGTCACACTCCCATTGTTGAGCTTCTCGTGTGCTACAAGGCCAACGTTAATCTCCAAGACCGATGGAAAAGAACACCCCTGACAGATGCAAGACTCTATGGACATCGGGATATATGCAGGATTCTTGAAGTGAATGGAGCCAATGACTTTACCAGTGATCAGTTGATGACCGTTCGACACGAACAAGATTCAAATGATGTGAAGTTTGACATGACGGAATTAGATACAGATAGTTCATCAGCGATTCAACAGGGTTCATTTGGTGAATCTGAAAAGGTGAAGTGGCGTGGAATCTGGGTCGTAAAGACCGTTGTCAAAAAAGAAATATACAAACTCCGAACAATATTGGATAAAAATGGCTTGCTAATTCTTCAATCTTCATACAGGATACTCTCTGCTAAGGATAATACTCTCCTACGGGAGCTTAGACATCCCAATATATTGCAGTTTCTTGGTTCAATTGTGCATCAAGAGGAGATGGTTCTAATTACCGAGTATCTACCAAAAGGCAATCTCGAAGATATTTTGACACAAAGAACACGTCTTGATGTGCCTACGGCTCTACGCTATGCACTTGATATTGCTAGGGGAATGAACTATCTTCATAAGCACGTGCCATCCCCAATAGTTCATAGCCATTTGAGTACCAGAAACTTGTTTCAGGATGAAGGTGGGCACTTGAAGATTGGGGAGTATTGGGTTCAAATGTTGCATGAACAAATTAACCCAGATCAAAATGGAAGGCAAAACAGTGATGGGACACAGGATTTAACCAAGATAGATATTTGGAGGTTCGGATTCATATATTATCAGATGCTAGAAGGAATGCACTTCCAGACCAACACAAACTCTGATCAACCCGAACTCGTTGATTTTAAACCGAAATTCCATTTAAGTCGATGTTCTAATAGAATTCAAGAGTTGATAGGGAAATGCACAGGTCATCCTTCTCGAAGACCCCCATTCGAAGAAGTCATACGTATCCTAGAAGAGGAACTATTGTCTGTGGGTAGAGGTGTATGTCCAGTTTACTGCGTACAGATGGAGATGGGCAACAAGTGCACCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
ko03320 ko04360 ko04510 ko05100 ko05213 map03320 map04360 map04510 map05100 map05213
Pfam Domains
Protein Families
Ank_2 Ank_4 Ank_5 Pkinase_Tyr

Protein Analysis

415

Amino Acids

47.66

Weight (kDa)

6.25

Isoelectric Point (pI)

40.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 31 - 101 7.9e-13 Ankyrin repeats (3 copies)
Ank_5 PF13857 38 - 85 2.7e-06 Ankyrin repeats (many copies)
Ank PF00023 47 - 79 1.9e-08 Ankyrin repeat
Ank_4 PF13637 52 - 100 2.4e-06 Ankyrin repeats (many copies)
Pkinase PF00069 177 - 383 8.7e-18 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 191 - 386 8.3e-24 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017270)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g27960
malus_domestica MD00G1053900.v1.1
prunus_persica Prupe.3G150700_v2.0.a1
pyrus_communis pycom17g25570
rosa_chinensis RchiOBHm_Chr5g0011771
rosa_laevigata RLG00000031861
rosa_roxburghii Rroxscaffold_1G00064780
rosa_rugosa Rorug04G0458900
rosa_samantha Rh5AG090600 Rh5BG086100 Rh5CG099900 Rh5DG086300
rosa_wichuraiana Rw5G007910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 24
AciI CCGC 2 cut(s) 22, 164
AclI AACGTT 1 cut(s) 219
AclWI GGATC 1 cut(s) 100
AcsI RAATTY 2 cut(s) 1058, 1086
AcvI CACGTG 1 cut(s) 787
AfaI GTAC 2 cut(s) 820, 1212
AfiI CCNNNNNNNGG 1 cut(s) 1165
AflIII ACRYGT 1 cut(s) 713
AgsI TTSAA 8 cut(s) 302, 366, 422, 548, 633, 856, 878, 1091
AjiI CACGTC 1 cut(s) 716
AluBI AGCT 3 cut(s) 63, 196, 598
AluI AGCT 3 cut(s) 63, 196, 598
Alw21I GWGCWC 1 cut(s) 1238
Alw44I GTGCAC 1 cut(s) 1234
AlwI GGATC 1 cut(s) 100
AoxI GGCC 1 cut(s) 213
ApaLI GTGCAC 1 cut(s) 1234
ApeKI GCWGC 1 cut(s) 154
ApoI RAATTY 2 cut(s) 1058, 1086
ArsI GACNNNNNNTTYG 2 cut(s) 700, 732
AspLEI GCGC 1 cut(s) 154
AsuHPI GGTGA 3 cut(s) 17, 449, 463
AsuII TTCGAA 1 cut(s) 1140
BaeGI GKGCMC 2 cut(s) 852, 1238
BarI GAAGNNNNNNTAC 2 cut(s) 1134, 1166
BauI CACGAG 1 cut(s) 200
BbrPI CACGTG 1 cut(s) 787
BbsI GAAGAC 1 cut(s) 1135
Bbv12I GWGCWC 1 cut(s) 1238
BbvI GCAGC 1 cut(s) 141
BccI CCATC 6 cut(s) 231, 646, 799, 923, 1210, 1216
BceAI ACGGC 2 cut(s) 165, 747
BciVI GTATCC 2 cut(s) 555, 1166
BclI TGATCA 2 cut(s) 331, 1027
BfaI CTAG 3 cut(s) 759, 992, 1160
BfoI RGCGCY 1 cut(s) 155
BfuI GTATCC 2 cut(s) 555, 1166
BisI GCNGC 2 cut(s) 155, 165
BlsI GCNGC 2 cut(s) 156, 166
BmgBI CACGTC 1 cut(s) 716
BmiI GGNNCC 1 cut(s) 313
BmsI GCATC 4 cut(s) 166, 250, 649, 978
BoxI GACNNNNGTC 1 cut(s) 485
BpiI GAAGAC 1 cut(s) 1135
Bpu10I CCTNAGC 1 cut(s) 573
Bpu14I TTCGAA 1 cut(s) 1140
BpuEI CTTGAG 1 cut(s) 79
BsaAI YACGTR 2 cut(s) 787, 1154
BsaBI GATNNNNATC 1 cut(s) 104
BsaJI CCNNGG 1 cut(s) 109
Bsc4I CCNNNNNNNGG 1 cut(s) 1165
Bse1I ACTGG 2 cut(s) 327, 1199
Bse8I GATNNNNATC 1 cut(s) 104
BseDI CCNNGG 1 cut(s) 109
BseGI GGATG 4 cut(s) 604, 791, 844, 1117
BseJI GATNNNNATC 1 cut(s) 104
BseLI CCNNNNNNNGG 1 cut(s) 1165
BseNI ACTGG 2 cut(s) 327, 1199
BseRI GAGGAG 1 cut(s) 662
BseSI GKGCMC 2 cut(s) 852, 1238
BseXI GCAGC 1 cut(s) 141
Bsh1236I CGCG 1 cut(s) 24
Bsh1285I CGRYCG 1 cut(s) 82
BshFI GGCC 1 cut(s) 215
BsiEI CGRYCG 1 cut(s) 82
BsiHKAI GWGCWC 1 cut(s) 1238
BslFI GGGAC 1 cut(s) 946
BslI CCNNNNNNNGG 1 cut(s) 1165
BsmFI GGGAC 1 cut(s) 946
BsmI GAATGC 1 cut(s) 1005
BsnI GGCC 1 cut(s) 215
Bsp119I TTCGAA 1 cut(s) 1140
Bsp1286I GDGCHC 2 cut(s) 852, 1238
Bsp143I GATC 4 cut(s) 105, 331, 904, 1027
BspACI CCGC 2 cut(s) 22, 164
BspANI GGCC 1 cut(s) 215
BspFNI CGCG 1 cut(s) 24
BspLI GGNNCC 1 cut(s) 313
BspPI GGATC 1 cut(s) 100
BspT104I TTCGAA 1 cut(s) 1140
BsrI ACTGG 2 cut(s) 327, 1199
BssECI CCNNGG 1 cut(s) 109
BssMI GATC 4 cut(s) 105, 331, 904, 1027
BssSI CACGAG 1 cut(s) 200
BssT1I CCWWGG 1 cut(s) 109
Bst2BI CACGAG 1 cut(s) 200
Bst4CI ACNGT 3 cut(s) 346, 484, 926
Bst6I CTCTTC 1 cut(s) 1158
BstAPI GCANNNNNTGC 1 cut(s) 1233
BstBAI YACGTR 2 cut(s) 787, 1154
BstBI TTCGAA 1 cut(s) 1140
BstC8I GCNNGC 2 cut(s) 22, 536
BstDEI CTNAG 2 cut(s) 573, 599
BstENI CCTNNNNNAGG 1 cut(s) 1163
BstF5I GGATG 4 cut(s) 604, 791, 844, 1117
BstFNI CGCG 1 cut(s) 24
BstH2I RGCGCY 1 cut(s) 155
BstHHI GCGC 1 cut(s) 154
BstKTI GATC 4 cut(s) 108, 334, 907, 1030
BstMBI GATC 4 cut(s) 105, 331, 904, 1027
BstMCI CGRYCG 1 cut(s) 82
BstMWI GCNNNNNNNGC 1 cut(s) 1233
BstNSI RCATGY 1 cut(s) 40
BstPAI GACNNNNGTC 1 cut(s) 485
BstSLI GKGCMC 2 cut(s) 852, 1238
BstSNI TACGTA 1 cut(s) 1154
BstUI CGCG 1 cut(s) 24
BstV1I GCAGC 1 cut(s) 141
BstV2I GAAGAC 1 cut(s) 1135
BsuI GTATCC 2 cut(s) 555, 1166
BsuRI GGCC 1 cut(s) 215
BtrI CACGTC 1 cut(s) 716
BtsCI GGATG 4 cut(s) 604, 791, 844, 1117
BtsIMutI CAGTG 2 cut(s) 334, 931
Cac8I GCNNGC 2 cut(s) 22, 536
CfoI GCGC 1 cut(s) 154
Csp6I GTAC 2 cut(s) 819, 1211
CviAII CATG 4 cut(s) 17, 37, 385, 887
CviJI RGCY 8 cut(s) 63, 196, 215, 314, 534, 598, 734, 810
CviKI_1 RGCY 8 cut(s) 63, 196, 215, 314, 534, 598, 734, 810
CviQI GTAC 2 cut(s) 819, 1211
DdeI CTNAG 2 cut(s) 573, 599
DpnI GATC 4 cut(s) 107, 333, 906, 1029
DpnII GATC 4 cut(s) 105, 331, 904, 1027
DraI TTTAAA 1 cut(s) 1051
Eam1104I CTCTTC 1 cut(s) 1158
EarI CTCTTC 1 cut(s) 1158
Eco105I TACGTA 1 cut(s) 1154
Eco130I CCWWGG 1 cut(s) 109
Eco72I CACGTG 1 cut(s) 787
EcoNI CCTNNNNNAGG 1 cut(s) 1163
EcoRI GAATTC 1 cut(s) 1086
EcoT14I CCWWGG 1 cut(s) 109
ErhI CCWWGG 1 cut(s) 109
FaeI CATG 4 cut(s) 20, 40, 388, 890
FaqI GGGAC 1 cut(s) 946
FatI CATG 4 cut(s) 16, 36, 384, 886
FauI CCCGC 1 cut(s) 29
FbaI TGATCA 2 cut(s) 331, 1027
Fnu4HI GCNGC 2 cut(s) 155, 165
FokI GGATG 4 cut(s) 591, 778, 851, 1104
Fsp4HI GCNGC 2 cut(s) 155, 165
FspBI CTAG 3 cut(s) 759, 992, 1160
GlaI GCGC 1 cut(s) 153
GluI GCNGC 2 cut(s) 155, 165
HaeII RGCGCY 1 cut(s) 155
HaeIII GGCC 1 cut(s) 215
HhaI GCGC 1 cut(s) 154
Hin1II CATG 4 cut(s) 20, 40, 388, 890
Hin6I GCGC 1 cut(s) 152
HinP1I GCGC 1 cut(s) 152
HinfI GANTC 8 cut(s) 10, 267, 295, 362, 418, 440, 465, 972
HphI GGTGA 3 cut(s) 17, 449, 463
Hpy166II GTNNAC 2 cut(s) 1204, 1236
Hpy188I TCNGA 5 cut(s) 445, 512, 971, 987, 1027
Hpy8I GTNNAC 2 cut(s) 1204, 1236
Hpy99I CGWCG 1 cut(s) 29
HpyAV CCTTC 4 cut(s) 836, 909, 989, 1131
HpyCH4III ACNGT 3 cut(s) 346, 484, 926
HpyCH4IV ACGT 4 cut(s) 219, 715, 786, 1153
HpyF10VI GCNNNNNNNGC 1 cut(s) 1233
HpyF3I CTNAG 2 cut(s) 573, 599
HpySE526I ACGT 4 cut(s) 219, 715, 786, 1153
Hsp92II CATG 4 cut(s) 20, 40, 388, 890
HspAI GCGC 1 cut(s) 152
Ksp22I TGATCA 2 cut(s) 331, 1027
Kzo9I GATC 4 cut(s) 105, 331, 904, 1027
LmnI GCTCC 2 cut(s) 311, 595
Lsp1109I GCAGC 1 cut(s) 141
LweI GCATC 4 cut(s) 166, 250, 649, 978
MaeI CTAG 3 cut(s) 759, 992, 1160
MaeII ACGT 4 cut(s) 219, 715, 786, 1153
MaeIII GTNAC 1 cut(s) 176
MalI GATC 4 cut(s) 107, 333, 906, 1029
MboI GATC 4 cut(s) 105, 331, 904, 1027
MboII GAAGA 8 cut(s) 536, 543, 704, 767, 868, 1140, 1154, 1175
MfeI CAATTG 1 cut(s) 633
MhlI GDGCHC 2 cut(s) 852, 1238
MluCI AATT 7 cut(s) 392, 540, 633, 660, 894, 1058, 1086
MlyI GAGTC 1 cut(s) 261
MmeI TCCRAC 1 cut(s) 67
MnlI CCTC 6 cut(s) 68, 166, 640, 957, 1159, 1181
MseI TTAA 5 cut(s) 222, 897, 944, 1050, 1067
MunI CAATTG 1 cut(s) 633
Mva1269I GAATGC 1 cut(s) 1005
MvnI CGCG 1 cut(s) 24
MwoI GCNNNNNNNGC 1 cut(s) 1233
NdeII GATC 4 cut(s) 105, 331, 904, 1027
NlaIII CATG 4 cut(s) 20, 40, 388, 890
NlaIV GGNNCC 1 cut(s) 313
NmuCI GTSAC 1 cut(s) 176
NspI RCATGY 1 cut(s) 40
NspV TTCGAA 1 cut(s) 1140
PcsI WCGNNNNNNNCGW 1 cut(s) 480
PctI GAATGC 1 cut(s) 1005
PfeI GAWTC 7 cut(s) 10, 295, 362, 418, 440, 465, 972
PkrI GCNGC 2 cut(s) 156, 166
PleI GAGTC 1 cut(s) 261
PmaCI CACGTG 1 cut(s) 787
PmlI CACGTG 1 cut(s) 787
PpsI GAGTC 1 cut(s) 261
Ppu21I YACGTR 2 cut(s) 787, 1154
PshAI GACNNNNGTC 1 cut(s) 485
Psp1406I AACGTT 1 cut(s) 219
PspCI CACGTG 1 cut(s) 787
PspN4I GGNNCC 1 cut(s) 313
RsaI GTAC 2 cut(s) 820, 1212
RsaNI GTAC 2 cut(s) 819, 1211
SaqAI TTAA 5 cut(s) 222, 897, 944, 1050, 1067
SatI GCNGC 2 cut(s) 155, 165
Sau3AI GATC 4 cut(s) 105, 331, 904, 1027
SchI GAGTC 1 cut(s) 261
SduI GDGCHC 2 cut(s) 852, 1238
SfaNI GCATC 4 cut(s) 166, 250, 649, 978
SfuI TTCGAA 1 cut(s) 1140
SmlI CTYRAG 1 cut(s) 58
SmoI CTYRAG 1 cut(s) 58
SnaBI TACGTA 1 cut(s) 1154
Sse9I AATT 7 cut(s) 392, 540, 633, 660, 894, 1058, 1086
SsiI CCGC 2 cut(s) 22, 164
SspI AATATT 1 cut(s) 519
SspMI CTAG 3 cut(s) 759, 992, 1160
StyI CCWWGG 1 cut(s) 109
TaaI ACNGT 3 cut(s) 346, 484, 926
TaiI ACGT 4 cut(s) 222, 718, 789, 1156
TaqI TCGA 7 cut(s) 33, 71, 349, 690, 1072, 1126, 1140
TaqII GACCGA 2 cut(s) 96, 249
TasI AATT 7 cut(s) 392, 540, 633, 660, 894, 1058, 1086
TauI GCSGC 1 cut(s) 167
TfiI GAWTC 7 cut(s) 10, 295, 362, 418, 440, 465, 972
Tru1I TTAA 5 cut(s) 222, 897, 944, 1050, 1067
Tru9I TTAA 5 cut(s) 222, 897, 944, 1050, 1067
TscAI CASTG 2 cut(s) 334, 931
TseFI GTSAC 1 cut(s) 176
TseI GCWGC 1 cut(s) 154
Tsp45I GTSAC 1 cut(s) 176
TspDTI ATGAA 9 cut(s) 399, 420, 543, 767, 782, 794, 855, 903, 964
TspGWI ACGGA 1 cut(s) 404
TspRI CASTG 2 cut(s) 334, 931
VneI GTGCAC 1 cut(s) 1234
XagI CCTNNNNNAGG 1 cut(s) 1163
XapI RAATTY 2 cut(s) 1058, 1086
XceI RCATGY 1 cut(s) 40
XspI CTAG 3 cut(s) 759, 992, 1160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.