RchiOBHm_Chr5g0011771

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0017270
Physical Location & Seq
Forward (+)
7806528 .. 7808834
2307 bp
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UTR
Exon/CDS
Intron
PRQ29238

Sequence Viewer

Length: 840 bp
ATGGGTGAGATTCTCCATGCCCGCGACGACTTCGACATGCAACTGATAGGGAAATTCTTGAGCTTCGCTTCGAGAGGCGACCGAGTTGGACTGAACCAGATGTTGATCCAAGGCATATCTCCTGATGTGCAAGACTACGATAATAGGACGGCGCTGCATCTGGCGGCGAGTGAGGGTCACACTCCCATTGTTGAGCTTCTCGTGTGCTACAAGGCCAACGTTAATCTCCAAGACCGATGGAAAAGAACTCCCCTGACAGATGCAAGACTCTATGGACATCGAGATATATGCAGGATTCTTGAAGTGAATGGAGCCAATGACTTTACCAGTGATCAGTTGATGGGTTCATTTGGTGAATCTGAAAAGGTCAAGTGGCGTGGAATCTGGGTCGTAAAGACCGTTGTCAAAAAAGACATATGTGCTCCTGTTAGGCCCGACCCTAGCACTGGGCATGCTTGGGGACAGCCCAGGACCGCAGAGCAAATGGGGCACCAAATGATACTCTCTGCTAAGGATAATACTCTCTTACGGGAGCTTAGACATCCCAATATATTGCAGTTTCTTGGTTCAATTGTGCAGCAGGAGGAGATGGTTCTAATTACCGAGTATCTACCAAAAGGCAATCTCGAGGATATTTTGACGCAAAGAACACGTCTTGATGTGCCTACGGCTCTACGCTATGCACTTGATATTGCTAGAAACTTGTTTCAGGATGAAGGTGGGCACTTGAAGATTGGGGAGTATTGGGTTCAAATGTTGCATGAACAAATTAACCCAGATCAAAATGGAAGTATGTATGTTGTATCAACTTCCATACATTTAGTCTTCAAATTTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
ko03320 ko04360 ko04510 ko05100 ko05213 map03320 map04360 map04510 map05100 map05213
Pfam Domains
Protein Families
Ank_2 Ank_4 Ank_5 Pkinase_Tyr

Protein Analysis

279

Amino Acids

31.78

Weight (kDa)

6.06

Isoelectric Point (pI)

31.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 31 - 101 3.9e-13 Ankyrin repeats (3 copies)
Ank_5 PF13857 38 - 85 1.4e-06 Ankyrin repeats (many copies)
Ank PF00023 47 - 79 1.1e-08 Ankyrin repeat
PK_Tyr_Ser-Thr PF07714 170 - 233 1.8e-10 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 172 - 233 1.4e-06 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017270)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g27960
malus_domestica MD00G1053900.v1.1
prunus_persica Prupe.3G150700_v2.0.a1
pyrus_communis pycom17g25570
rosa_chinensis RchiOBHm_Chr5g0011771
rosa_laevigata RLG00000031861
rosa_roxburghii Rroxscaffold_1G00064780
rosa_rugosa Rorug04G0458900
rosa_samantha Rh5AG090600 Rh5BG086100 Rh5CG099900 Rh5DG086300
rosa_wichuraiana Rw5G007910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 489
AccII CGCG 1 cut(s) 24
AciI CCGC 3 cut(s) 22, 164, 474
AclI AACGTT 1 cut(s) 219
AclWI GGATC 1 cut(s) 100
AcsI RAATTY 2 cut(s) 53, 830
AfiI CCNNNNNNNGG 1 cut(s) 446
AflIII ACRYGT 1 cut(s) 650
AgsI TTSAA 5 cut(s) 302, 570, 730, 752, 829
AjiI CACGTC 1 cut(s) 653
AjnI CCWGG 1 cut(s) 467
AluBI AGCT 3 cut(s) 63, 196, 535
AluI AGCT 3 cut(s) 63, 196, 535
Alw21I GWGCWC 1 cut(s) 424
AlwI GGATC 1 cut(s) 100
Ama87I CYCGRG 1 cut(s) 626
AoxI GGCC 2 cut(s) 213, 431
ApeKI GCWGC 2 cut(s) 154, 577
ApoI RAATTY 2 cut(s) 53, 830
ArsI GACNNNNNNTTYG 2 cut(s) 637, 669
AspLEI GCGC 1 cut(s) 154
AspS9I GGNCC 2 cut(s) 432, 471
AsuHPI GGTGA 2 cut(s) 17, 365
AvaI CYCGRG 1 cut(s) 626
AvaII GGWCC 1 cut(s) 471
BaeGI GKGCMC 2 cut(s) 492, 726
BanI GGYRCC 1 cut(s) 489
BauI CACGAG 1 cut(s) 200
BbsI GAAGAC 1 cut(s) 817
Bbv12I GWGCWC 1 cut(s) 424
BbvI GCAGC 2 cut(s) 141, 589
BccI CCATC 3 cut(s) 231, 334, 583
BceAI ACGGC 2 cut(s) 165, 684
BcgI CGANNNNNNTGC 2 cut(s) 270, 304
BciT130I CCWGG 1 cut(s) 469
BclI TGATCA 1 cut(s) 331
BfaI CTAG 2 cut(s) 441, 696
BfoI RGCGCY 1 cut(s) 155
BisI GCNGC 3 cut(s) 155, 165, 578
BlsI GCNGC 3 cut(s) 156, 166, 579
Bme1390I CCNGG 1 cut(s) 469
Bme18I GGWCC 1 cut(s) 471
BmeT110I CYCGRG 1 cut(s) 626
BmgBI CACGTC 1 cut(s) 653
BmgT120I GGNCC 2 cut(s) 432, 471
BmiI GGNNCC 2 cut(s) 313, 491
BmrFI CCNGG 1 cut(s) 469
BmrI ACTGGG 1 cut(s) 456
BmsI GCATC 2 cut(s) 166, 250
BmuI ACTGGG 1 cut(s) 456
BoxI GACNNNNGTC 1 cut(s) 401
BpiI GAAGAC 1 cut(s) 817
Bpu10I CCTNAGC 1 cut(s) 510
BpuEI CTTGAG 1 cut(s) 79
BsaBI GATNNNNATC 1 cut(s) 104
BsaJI CCNNGG 2 cut(s) 109, 467
Bsc4I CCNNNNNNNGG 1 cut(s) 446
Bse1I ACTGG 2 cut(s) 327, 451
Bse8I GATNNNNATC 1 cut(s) 104
BseBI CCWGG 1 cut(s) 469
BseDI CCNNGG 2 cut(s) 109, 467
BseGI GGATG 2 cut(s) 541, 718
BseJI GATNNNNATC 1 cut(s) 104
BseLI CCNNNNNNNGG 1 cut(s) 446
BseNI ACTGG 2 cut(s) 327, 451
BseRI GAGGAG 1 cut(s) 599
BseSI GKGCMC 2 cut(s) 492, 726
BseXI GCAGC 2 cut(s) 141, 589
BsgI GTGCAG 1 cut(s) 596
Bsh1236I CGCG 1 cut(s) 24
Bsh1285I CGRYCG 1 cut(s) 82
BshFI GGCC 2 cut(s) 215, 433
BshNI GGYRCC 1 cut(s) 489
BsiEI CGRYCG 1 cut(s) 82
BsiHKAI GWGCWC 1 cut(s) 424
BsiHKCI CYCGRG 1 cut(s) 626
BslFI GGGAC 1 cut(s) 474
BslI CCNNNNNNNGG 1 cut(s) 446
BsmFI GGGAC 1 cut(s) 474
BsnI GGCC 2 cut(s) 215, 433
BsoBI CYCGRG 1 cut(s) 626
Bsp1286I GDGCHC 3 cut(s) 424, 492, 726
Bsp143I GATC 3 cut(s) 105, 331, 778
BspACI CCGC 3 cut(s) 22, 164, 474
BspANI GGCC 2 cut(s) 215, 433
BspFNI CGCG 1 cut(s) 24
BspLI GGNNCC 2 cut(s) 313, 491
BspPI GGATC 1 cut(s) 100
BspT107I GGYRCC 1 cut(s) 489
BsrI ACTGG 2 cut(s) 327, 451
BssECI CCNNGG 2 cut(s) 109, 467
BssMI GATC 3 cut(s) 105, 331, 778
BssSI CACGAG 1 cut(s) 200
BssT1I CCWWGG 1 cut(s) 109
Bst2BI CACGAG 1 cut(s) 200
Bst2UI CCWGG 1 cut(s) 469
Bst4CI ACNGT 1 cut(s) 400
BstC8I GCNNGC 2 cut(s) 22, 453
BstDEI CTNAG 2 cut(s) 510, 536
BstF5I GGATG 2 cut(s) 541, 718
BstFNI CGCG 1 cut(s) 24
BstH2I RGCGCY 1 cut(s) 155
BstHHI GCGC 1 cut(s) 154
BstKTI GATC 3 cut(s) 108, 334, 781
BstMBI GATC 3 cut(s) 105, 331, 778
BstMCI CGRYCG 1 cut(s) 82
BstMWI GCNNNNNNNGC 1 cut(s) 487
BstNI CCWGG 1 cut(s) 469
BstNSI RCATGY 2 cut(s) 40, 455
BstPAI GACNNNNGTC 1 cut(s) 401
BstSCI CCNGG 1 cut(s) 467
BstSLI GKGCMC 2 cut(s) 492, 726
BstUI CGCG 1 cut(s) 24
BstV1I GCAGC 2 cut(s) 141, 589
BstV2I GAAGAC 1 cut(s) 817
BsuRI GGCC 2 cut(s) 215, 433
BtrI CACGTC 1 cut(s) 653
BtsCI GGATG 2 cut(s) 541, 718
BtsIMutI CAGTG 2 cut(s) 334, 444
Cac8I GCNNGC 2 cut(s) 22, 453
CfoI GCGC 1 cut(s) 154
Cfr13I GGNCC 2 cut(s) 432, 471
CseI GACGC 1 cut(s) 649
CspCI CAANNNNNGTGG 2 cut(s) 358, 393
CviAII CATG 4 cut(s) 17, 37, 452, 761
CviJI RGCY 8 cut(s) 63, 196, 215, 314, 433, 466, 535, 671
CviKI_1 RGCY 8 cut(s) 63, 196, 215, 314, 433, 466, 535, 671
DdeI CTNAG 2 cut(s) 510, 536
DpnI GATC 3 cut(s) 107, 333, 780
DpnII GATC 3 cut(s) 105, 331, 778
Eco130I CCWWGG 1 cut(s) 109
Eco47I GGWCC 1 cut(s) 471
Eco88I CYCGRG 1 cut(s) 626
EcoRII CCWGG 1 cut(s) 467
EcoT14I CCWWGG 1 cut(s) 109
ErhI CCWWGG 1 cut(s) 109
FaeI CATG 4 cut(s) 20, 40, 455, 764
FaqI GGGAC 1 cut(s) 474
FatI CATG 4 cut(s) 16, 36, 451, 760
FauI CCCGC 1 cut(s) 29
FauNDI CATATG 1 cut(s) 416
FbaI TGATCA 1 cut(s) 331
Fnu4HI GCNGC 3 cut(s) 155, 165, 578
FokI GGATG 2 cut(s) 528, 725
Fsp4HI GCNGC 3 cut(s) 155, 165, 578
FspBI CTAG 2 cut(s) 441, 696
GlaI GCGC 1 cut(s) 153
GluI GCNGC 3 cut(s) 155, 165, 578
HaeII RGCGCY 1 cut(s) 155
HaeIII GGCC 2 cut(s) 215, 433
HgaI GACGC 1 cut(s) 649
HhaI GCGC 1 cut(s) 154
Hin1II CATG 4 cut(s) 20, 40, 455, 764
Hin6I GCGC 1 cut(s) 152
HinP1I GCGC 1 cut(s) 152
HinfI GANTC 5 cut(s) 10, 267, 295, 356, 381
HphI GGTGA 2 cut(s) 17, 365
Hpy188I TCNGA 1 cut(s) 361
Hpy188III TCNNGA 8 cut(s) 58, 72, 122, 281, 299, 626, 656, 710
Hpy99I CGWCG 1 cut(s) 29
HpyAV CCTTC 1 cut(s) 710
HpyCH4III ACNGT 1 cut(s) 400
HpyCH4IV ACGT 2 cut(s) 219, 652
HpyCH4V TGCA 9 cut(s) 40, 130, 157, 263, 291, 556, 577, 683, 760
HpyF10VI GCNNNNNNNGC 1 cut(s) 487
HpyF3I CTNAG 2 cut(s) 510, 536
HpySE526I ACGT 2 cut(s) 219, 652
Hsp92II CATG 4 cut(s) 20, 40, 455, 764
HspAI GCGC 1 cut(s) 152
Ksp22I TGATCA 1 cut(s) 331
Kzo9I GATC 3 cut(s) 105, 331, 778
LmnI GCTCC 3 cut(s) 311, 427, 532
Lsp1109I GCAGC 2 cut(s) 141, 589
LweI GCATC 2 cut(s) 166, 250
MaeI CTAG 2 cut(s) 441, 696
MaeII ACGT 2 cut(s) 219, 652
MaeIII GTNAC 1 cut(s) 176
MalI GATC 3 cut(s) 107, 333, 780
MboI GATC 3 cut(s) 105, 331, 778
MboII GAAGA 2 cut(s) 742, 817
MfeI CAATTG 1 cut(s) 570
MhlI GDGCHC 3 cut(s) 424, 492, 726
MluCI AATT 5 cut(s) 53, 570, 597, 768, 830
MlyI GAGTC 1 cut(s) 261
MmeI TCCRAC 1 cut(s) 67
MnlI CCTC 4 cut(s) 68, 166, 577, 622
MseI TTAA 2 cut(s) 222, 771
MspR9I CCNGG 1 cut(s) 469
MunI CAATTG 1 cut(s) 570
MvaI CCWGG 1 cut(s) 469
MvnI CGCG 1 cut(s) 24
MwoI GCNNNNNNNGC 1 cut(s) 487
NdeI CATATG 1 cut(s) 416
NdeII GATC 3 cut(s) 105, 331, 778
NlaIII CATG 4 cut(s) 20, 40, 455, 764
NlaIV GGNNCC 2 cut(s) 313, 491
NmuCI GTSAC 1 cut(s) 176
NspI RCATGY 2 cut(s) 40, 455
PaeI GCATGC 1 cut(s) 455
PaeR7I CTCGAG 1 cut(s) 626
PcsI WCGNNNNNNNCGW 1 cut(s) 396
PfeI GAWTC 4 cut(s) 10, 295, 356, 381
PkrI GCNGC 3 cut(s) 156, 166, 579
PleI GAGTC 1 cut(s) 261
PpsI GAGTC 1 cut(s) 261
PshAI GACNNNNGTC 1 cut(s) 401
Psp1406I AACGTT 1 cut(s) 219
Psp6I CCWGG 1 cut(s) 467
PspGI CCWGG 1 cut(s) 467
PspN4I GGNNCC 2 cut(s) 313, 491
PspPI GGNCC 2 cut(s) 432, 471
SaqAI TTAA 2 cut(s) 222, 771
SatI GCNGC 3 cut(s) 155, 165, 578
Sau3AI GATC 3 cut(s) 105, 331, 778
Sau96I GGNCC 2 cut(s) 432, 471
SchI GAGTC 1 cut(s) 261
ScrFI CCNGG 1 cut(s) 469
SduI GDGCHC 3 cut(s) 424, 492, 726
SetI ASST 7 cut(s) 65, 198, 222, 369, 537, 655, 721
SfaNI GCATC 2 cut(s) 166, 250
Sfr274I CTCGAG 1 cut(s) 626
SinI GGWCC 1 cut(s) 471
SlaI CTCGAG 1 cut(s) 626
SmlI CTYRAG 2 cut(s) 58, 626
SmoI CTYRAG 2 cut(s) 58, 626
SphI GCATGC 1 cut(s) 455
Sse9I AATT 5 cut(s) 53, 570, 597, 768, 830
SsiI CCGC 3 cut(s) 22, 164, 474
SspMI CTAG 2 cut(s) 441, 696
StyD4I CCNGG 1 cut(s) 467
StyI CCWWGG 1 cut(s) 109
TaaI ACNGT 1 cut(s) 400
TaiI ACGT 2 cut(s) 222, 655
TaqI TCGA 4 cut(s) 33, 71, 280, 627
TaqII GACCGA 2 cut(s) 96, 249
TasI AATT 5 cut(s) 53, 570, 597, 768, 830
TauI GCSGC 1 cut(s) 167
TfiI GAWTC 4 cut(s) 10, 295, 356, 381
Tru1I TTAA 2 cut(s) 222, 771
Tru9I TTAA 2 cut(s) 222, 771
TscAI CASTG 2 cut(s) 334, 451
TseFI GTSAC 1 cut(s) 176
TseI GCWGC 2 cut(s) 154, 577
Tsp45I GTSAC 1 cut(s) 176
TspDTI ATGAA 3 cut(s) 336, 729, 777
TspRI CASTG 2 cut(s) 334, 451
VpaK11BI GGWCC 1 cut(s) 471
XapI RAATTY 2 cut(s) 53, 830
XceI RCATGY 2 cut(s) 40, 455
XhoI CTCGAG 1 cut(s) 626
XspI CTAG 2 cut(s) 441, 696
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.