Rh5BG086100

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0017270
Physical Location & Seq
Forward (+)
7458322 .. 7462046
3725 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG086100.1

Sequence Viewer

Length: 1275 bp
ATGAAGACGATGGGTGAGATTCTCCATGCCCGCGACGACTTCGACATGCAACTGATAGGGAAATTCTTGAGCTTCGCTTCGAGAGGCGACCGAGTTGGACTGAACCAGATGTTGATCCAAGGCATATCTCCTGATGTGCAAGACTACGATAATAGGACGGCGCTGCATCTGGCGGCGAGTGAGGGTCACACTCCCATTGTTGAGCTTCTCGTGTGCTACAAGGCCAACGTTAATCTCCAAGACCGATGGAAAAGAACTCCCCTGACAGATGCAAGACTCTATGGACATCGAGATATATGCAGGATTCTTGAAGTGAATGGAGCCAATGACTTTACCAGTGATCAGTTGATGACCGTTCGACACGAACAAGATTCAAATGATGTGAAGTTTGACATGACGGAATTAGATACAGATAGTTCATCAGCGATTCAACAGGGTTCATTTGGTGAATCTGAAAAGGTGAAGTGGCGTGGAATCTGGGTCGTAAAGACCGTCGTCAAAAAAGACATATGTGCTCCTGTTAGGCCCGACCCTAGCACTGGGCATGCTTGGCGACAGCCCAGGGCCGCAGAGCAAATGGGGCACCAAATGATACTCTCTGCTAAGGATAATACTCTCTTACGGGAGCTTAGACATCCCAATATATTGCAGTTTCTTGGTTCAATTGTGCAGCAAGAGGAGATGGTTCTAATTACCGAGTATCTACCAAAAGGCAATCTCGAGGATATTTTGACGCAAAGAACACGTCTTGATGTGCCTACGGCTCTACGCTATGCACTTGATATTGCTAGGGGAATGAACTATCTTCATAAGCACGTGCCATCCCCAATAGTTCACAGCCATTTGAGTACCAGAAACTTATTTCAGGATGAAGGTGGGCACTTGAAGATTGGGGAGTACTGGGTTCAAATGTTGCATGAACAAATTAACCCAGATCAAAATGGAAGGCAAAACAGTGATGGGACACAGGATTTAACCAAGATAGATATTTGGAGGTTCGGATTCATATATTATCAGATGCTAGAAGGAATGCACTTCCAGACCAACACAAACTCTGATCAACCCGAACTCGTTGATTTTAAACCGAAATTCCATTTAAGTCGATGTTCTAATAGAATTCAAGAGTTGATAGGGAAATGCTCAGGTCATCCTTCTCGAAGACCCCCATTCGAAGAAGTCATACGTATCCTAGAAGAGGAACTATTGTCTGTGGGTAGAGGTGTGTGTCCAGTTTACTGCGTACAGATGGAGATGGGCAACAAGTGCACCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
ko03320 ko04360 ko04510 ko05100 ko05213 map03320 map04360 map04510 map05100 map05213
Pfam Domains
Protein Families
Ank_2 Ank_4 Ank_5 Pkinase_Tyr

Protein Analysis

424

Amino Acids

48.61

Weight (kDa)

6.19

Isoelectric Point (pI)

39.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 34 - 104 8.2e-13 Ankyrin repeats (3 copies)
Ank_5 PF13857 41 - 88 2.8e-06 Ankyrin repeats (many copies)
Ank PF00023 50 - 82 2e-08 Ankyrin repeat
Ank_4 PF13637 55 - 103 2.4e-06 Ankyrin repeats (many copies)
PK_Tyr_Ser-Thr PF07714 202 - 396 1.3e-23 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 203 - 393 1.8e-17 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017270)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g27960
malus_domestica MD00G1053900.v1.1
prunus_persica Prupe.3G150700_v2.0.a1
pyrus_communis pycom17g25570
rosa_chinensis RchiOBHm_Chr5g0011771
rosa_laevigata RLG00000031861
rosa_roxburghii Rroxscaffold_1G00064780
rosa_rugosa Rorug04G0458900
rosa_samantha Rh5AG090600 Rh5BG086100 Rh5CG099900 Rh5DG086300
rosa_wichuraiana Rw5G007910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 582
AccII CGCG 1 cut(s) 33
AciI CCGC 3 cut(s) 31, 173, 567
AclI AACGTT 1 cut(s) 228
AclWI GGATC 1 cut(s) 109
AcsI RAATTY 3 cut(s) 62, 1088, 1116
AcvI CACGTG 1 cut(s) 817
AfaI GTAC 3 cut(s) 850, 899, 1242
AfiI CCNNNNNNNGG 2 cut(s) 539, 1195
AflIII ACRYGT 1 cut(s) 743
AgsI TTSAA 7 cut(s) 311, 375, 431, 663, 886, 908, 1121
AjiI CACGTC 1 cut(s) 746
AjnI CCWGG 1 cut(s) 560
AluBI AGCT 3 cut(s) 72, 205, 628
AluI AGCT 3 cut(s) 72, 205, 628
Alw21I GWGCWC 2 cut(s) 517, 1268
Alw44I GTGCAC 1 cut(s) 1264
AlwI GGATC 1 cut(s) 109
Ama87I CYCGRG 1 cut(s) 719
AoxI GGCC 3 cut(s) 222, 524, 564
ApaLI GTGCAC 1 cut(s) 1264
ApeKI GCWGC 2 cut(s) 163, 670
ApoI RAATTY 3 cut(s) 62, 1088, 1116
ArsI GACNNNNNNTTYG 2 cut(s) 730, 762
AspLEI GCGC 1 cut(s) 163
AspS9I GGNCC 2 cut(s) 525, 564
AsuHPI GGTGA 3 cut(s) 26, 458, 472
AsuII TTCGAA 1 cut(s) 1170
AvaI CYCGRG 1 cut(s) 719
BaeGI GKGCMC 3 cut(s) 585, 882, 1268
BanI GGYRCC 1 cut(s) 582
BarI GAAGNNNNNNTAC 2 cut(s) 1164, 1196
BauI CACGAG 1 cut(s) 209
BbrPI CACGTG 1 cut(s) 817
BbsI GAAGAC 2 cut(s) 11, 1165
Bbv12I GWGCWC 2 cut(s) 517, 1268
BbvI GCAGC 2 cut(s) 150, 682
BccI CCATC 7 cut(s) 4, 240, 676, 829, 953, 1240, 1246
BceAI ACGGC 2 cut(s) 174, 777
BcgI CGANNNNNNTGC 2 cut(s) 279, 313
BciT130I CCWGG 1 cut(s) 562
BciVI GTATCC 1 cut(s) 1196
BclI TGATCA 2 cut(s) 340, 1057
BfaI CTAG 4 cut(s) 534, 789, 1022, 1190
BfoI RGCGCY 1 cut(s) 164
BfuI GTATCC 1 cut(s) 1196
BisI GCNGC 4 cut(s) 164, 174, 567, 671
BlsI GCNGC 4 cut(s) 165, 175, 568, 672
BmcAI AGTACT 1 cut(s) 899
Bme1390I CCNGG 1 cut(s) 562
BmeT110I CYCGRG 1 cut(s) 719
BmgBI CACGTC 1 cut(s) 746
BmgT120I GGNCC 2 cut(s) 525, 564
BmiI GGNNCC 2 cut(s) 322, 584
BmrFI CCNGG 1 cut(s) 562
BmrI ACTGGG 2 cut(s) 549, 910
BmsI GCATC 3 cut(s) 175, 259, 1008
BmuI ACTGGG 2 cut(s) 549, 910
BoxI GACNNNNGTC 1 cut(s) 494
BpiI GAAGAC 2 cut(s) 11, 1165
Bpu10I CCTNAGC 2 cut(s) 603, 1141
Bpu14I TTCGAA 1 cut(s) 1170
BpuEI CTTGAG 1 cut(s) 88
BsaAI YACGTR 2 cut(s) 817, 1184
BsaBI GATNNNNATC 1 cut(s) 113
BsaJI CCNNGG 3 cut(s) 118, 560, 561
Bsc4I CCNNNNNNNGG 2 cut(s) 539, 1195
Bse1I ACTGG 4 cut(s) 336, 544, 905, 1229
Bse8I GATNNNNATC 1 cut(s) 113
BseBI CCWGG 1 cut(s) 562
BseDI CCNNGG 3 cut(s) 118, 560, 561
BseGI GGATG 4 cut(s) 634, 821, 874, 1147
BseJI GATNNNNATC 1 cut(s) 113
BseLI CCNNNNNNNGG 2 cut(s) 539, 1195
BseMII CTCAG 1 cut(s) 1155
BseNI ACTGG 4 cut(s) 336, 544, 905, 1229
BseRI GAGGAG 1 cut(s) 692
BseSI GKGCMC 3 cut(s) 585, 882, 1268
BseXI GCAGC 2 cut(s) 150, 682
BsgI GTGCAG 1 cut(s) 689
Bsh1236I CGCG 1 cut(s) 33
Bsh1285I CGRYCG 1 cut(s) 91
BshFI GGCC 3 cut(s) 224, 526, 566
BshNI GGYRCC 1 cut(s) 582
BsiEI CGRYCG 1 cut(s) 91
BsiHKAI GWGCWC 2 cut(s) 517, 1268
BsiHKCI CYCGRG 1 cut(s) 719
BslFI GGGAC 1 cut(s) 976
BslI CCNNNNNNNGG 2 cut(s) 539, 1195
BsmFI GGGAC 1 cut(s) 976
BsmI GAATGC 1 cut(s) 1035
BsnI GGCC 3 cut(s) 224, 526, 566
BsoBI CYCGRG 1 cut(s) 719
Bsp119I TTCGAA 1 cut(s) 1170
Bsp1286I GDGCHC 4 cut(s) 517, 585, 882, 1268
Bsp143I GATC 4 cut(s) 114, 340, 934, 1057
BspACI CCGC 3 cut(s) 31, 173, 567
BspANI GGCC 3 cut(s) 224, 526, 566
BspCNI CTCAG 1 cut(s) 1154
BspFNI CGCG 1 cut(s) 33
BspLI GGNNCC 2 cut(s) 322, 584
BspPI GGATC 1 cut(s) 109
BspT104I TTCGAA 1 cut(s) 1170
BspT107I GGYRCC 1 cut(s) 582
BsrI ACTGG 4 cut(s) 336, 544, 905, 1229
BssECI CCNNGG 3 cut(s) 118, 560, 561
BssMI GATC 4 cut(s) 114, 340, 934, 1057
BssSI CACGAG 1 cut(s) 209
BssT1I CCWWGG 1 cut(s) 118
Bst2BI CACGAG 1 cut(s) 209
Bst2UI CCWGG 1 cut(s) 562
Bst4CI ACNGT 3 cut(s) 355, 493, 956
Bst6I CTCTTC 1 cut(s) 1188
BstAPI GCANNNNNTGC 1 cut(s) 1263
BstBAI YACGTR 2 cut(s) 817, 1184
BstBI TTCGAA 1 cut(s) 1170
BstC8I GCNNGC 2 cut(s) 31, 546
BstDEI CTNAG 3 cut(s) 603, 629, 1141
BstENI CCTNNNNNAGG 1 cut(s) 1193
BstF5I GGATG 4 cut(s) 634, 821, 874, 1147
BstFNI CGCG 1 cut(s) 33
BstH2I RGCGCY 1 cut(s) 164
BstHHI GCGC 1 cut(s) 163
BstKTI GATC 4 cut(s) 117, 343, 937, 1060
BstMBI GATC 4 cut(s) 114, 340, 934, 1057
BstMCI CGRYCG 1 cut(s) 91
BstMWI GCNNNNNNNGC 3 cut(s) 550, 580, 1263
BstNI CCWGG 1 cut(s) 562
BstNSI RCATGY 2 cut(s) 49, 548
BstPAI GACNNNNGTC 1 cut(s) 494
BstSCI CCNGG 1 cut(s) 560
BstSLI GKGCMC 3 cut(s) 585, 882, 1268
BstSNI TACGTA 1 cut(s) 1184
BstUI CGCG 1 cut(s) 33
BstV1I GCAGC 2 cut(s) 150, 682
BstV2I GAAGAC 2 cut(s) 11, 1165
BsuI GTATCC 1 cut(s) 1196
BsuRI GGCC 3 cut(s) 224, 526, 566
BtrI CACGTC 1 cut(s) 746
BtsCI GGATG 4 cut(s) 634, 821, 874, 1147
BtsIMutI CAGTG 3 cut(s) 343, 537, 961
Cac8I GCNNGC 2 cut(s) 31, 546
CfoI GCGC 1 cut(s) 163
Cfr13I GGNCC 2 cut(s) 525, 564
CseI GACGC 1 cut(s) 742
Csp6I GTAC 3 cut(s) 849, 898, 1241
CviAII CATG 5 cut(s) 26, 46, 394, 545, 917
CviQI GTAC 3 cut(s) 849, 898, 1241
DdeI CTNAG 3 cut(s) 603, 629, 1141
DpnI GATC 4 cut(s) 116, 342, 936, 1059
DpnII GATC 4 cut(s) 114, 340, 934, 1057
DraI TTTAAA 1 cut(s) 1081
Eam1104I CTCTTC 1 cut(s) 1188
EarI CTCTTC 1 cut(s) 1188
Eco105I TACGTA 1 cut(s) 1184
Eco130I CCWWGG 1 cut(s) 118
Eco72I CACGTG 1 cut(s) 817
Eco88I CYCGRG 1 cut(s) 719
EcoNI CCTNNNNNAGG 1 cut(s) 1193
EcoRI GAATTC 1 cut(s) 1116
EcoRII CCWGG 1 cut(s) 560
EcoT14I CCWWGG 1 cut(s) 118
ErhI CCWWGG 1 cut(s) 118
FaeI CATG 5 cut(s) 29, 49, 397, 548, 920
FaqI GGGAC 1 cut(s) 976
FatI CATG 5 cut(s) 25, 45, 393, 544, 916
FauI CCCGC 1 cut(s) 38
FauNDI CATATG 1 cut(s) 509
FbaI TGATCA 2 cut(s) 340, 1057
Fnu4HI GCNGC 4 cut(s) 164, 174, 567, 671
FokI GGATG 4 cut(s) 621, 808, 881, 1134
Fsp4HI GCNGC 4 cut(s) 164, 174, 567, 671
FspBI CTAG 4 cut(s) 534, 789, 1022, 1190
GlaI GCGC 1 cut(s) 162
GluI GCNGC 4 cut(s) 164, 174, 567, 671
HaeII RGCGCY 1 cut(s) 164
HaeIII GGCC 3 cut(s) 224, 526, 566
HgaI GACGC 1 cut(s) 742
HhaI GCGC 1 cut(s) 163
Hin1II CATG 5 cut(s) 29, 49, 397, 548, 920
Hin6I GCGC 1 cut(s) 161
HinP1I GCGC 1 cut(s) 161
HinfI GANTC 8 cut(s) 19, 276, 304, 371, 427, 449, 474, 1002
HphI GGTGA 3 cut(s) 26, 458, 472
Hpy166II GTNNAC 3 cut(s) 835, 1234, 1266
Hpy188I TCNGA 4 cut(s) 454, 1001, 1017, 1057
Hpy8I GTNNAC 3 cut(s) 835, 1234, 1266
Hpy99I CGWCG 2 cut(s) 38, 497
HpyAV CCTTC 4 cut(s) 866, 939, 1019, 1161
HpyCH4III ACNGT 3 cut(s) 355, 493, 956
HpyCH4IV ACGT 4 cut(s) 228, 745, 816, 1183
HpyF10VI GCNNNNNNNGC 3 cut(s) 550, 580, 1263
HpyF3I CTNAG 3 cut(s) 603, 629, 1141
HpySE526I ACGT 4 cut(s) 228, 745, 816, 1183
Hsp92II CATG 5 cut(s) 29, 49, 397, 548, 920
HspAI GCGC 1 cut(s) 161
Ksp22I TGATCA 2 cut(s) 340, 1057
Kzo9I GATC 4 cut(s) 114, 340, 934, 1057
LmnI GCTCC 3 cut(s) 320, 520, 625
Lsp1109I GCAGC 2 cut(s) 150, 682
LweI GCATC 3 cut(s) 175, 259, 1008
MaeI CTAG 4 cut(s) 534, 789, 1022, 1190
MaeII ACGT 4 cut(s) 228, 745, 816, 1183
MaeIII GTNAC 1 cut(s) 185
MalI GATC 4 cut(s) 116, 342, 936, 1059
MboI GATC 4 cut(s) 114, 340, 934, 1057
MboII GAAGA 6 cut(s) 16, 797, 898, 1170, 1184, 1205
MfeI CAATTG 1 cut(s) 663
MhlI GDGCHC 4 cut(s) 517, 585, 882, 1268
MluCI AATT 7 cut(s) 62, 401, 663, 690, 924, 1088, 1116
MlyI GAGTC 1 cut(s) 270
MmeI TCCRAC 1 cut(s) 76
MnlI CCTC 7 cut(s) 77, 175, 670, 715, 987, 1189, 1211
MseI TTAA 5 cut(s) 231, 927, 974, 1080, 1097
MspR9I CCNGG 1 cut(s) 562
MunI CAATTG 1 cut(s) 663
Mva1269I GAATGC 1 cut(s) 1035
MvaI CCWGG 1 cut(s) 562
MvnI CGCG 1 cut(s) 33
MwoI GCNNNNNNNGC 3 cut(s) 550, 580, 1263
NdeI CATATG 1 cut(s) 509
NdeII GATC 4 cut(s) 114, 340, 934, 1057
NlaIII CATG 5 cut(s) 29, 49, 397, 548, 920
NlaIV GGNNCC 2 cut(s) 322, 584
NmuCI GTSAC 1 cut(s) 185
NspI RCATGY 2 cut(s) 49, 548
NspV TTCGAA 1 cut(s) 1170
PaeI GCATGC 1 cut(s) 548
PaeR7I CTCGAG 1 cut(s) 719
PasI CCCWGGG 1 cut(s) 561
PcsI WCGNNNNNNNCGW 1 cut(s) 489
PctI GAATGC 1 cut(s) 1035
PfeI GAWTC 7 cut(s) 19, 304, 371, 427, 449, 474, 1002
PkrI GCNGC 4 cut(s) 165, 175, 568, 672
PleI GAGTC 1 cut(s) 270
PmaCI CACGTG 1 cut(s) 817
PmlI CACGTG 1 cut(s) 817
PpsI GAGTC 1 cut(s) 270
Ppu21I YACGTR 2 cut(s) 817, 1184
PshAI GACNNNNGTC 1 cut(s) 494
Psp1406I AACGTT 1 cut(s) 228
Psp6I CCWGG 1 cut(s) 560
PspCI CACGTG 1 cut(s) 817
PspGI CCWGG 1 cut(s) 560
PspN4I GGNNCC 2 cut(s) 322, 584
PspPI GGNCC 2 cut(s) 525, 564
RsaI GTAC 3 cut(s) 850, 899, 1242
RsaNI GTAC 3 cut(s) 849, 898, 1241
SaqAI TTAA 5 cut(s) 231, 927, 974, 1080, 1097
SatI GCNGC 4 cut(s) 164, 174, 567, 671
Sau3AI GATC 4 cut(s) 114, 340, 934, 1057
Sau96I GGNCC 2 cut(s) 525, 564
ScaI AGTACT 1 cut(s) 899
SchI GAGTC 1 cut(s) 270
ScrFI CCNGG 1 cut(s) 562
SduI GDGCHC 4 cut(s) 517, 585, 882, 1268
SfaNI GCATC 3 cut(s) 175, 259, 1008
Sfr274I CTCGAG 1 cut(s) 719
SfuI TTCGAA 1 cut(s) 1170
SlaI CTCGAG 1 cut(s) 719
SmlI CTYRAG 2 cut(s) 67, 719
SmoI CTYRAG 2 cut(s) 67, 719
SnaBI TACGTA 1 cut(s) 1184
SphI GCATGC 1 cut(s) 548
Sse9I AATT 7 cut(s) 62, 401, 663, 690, 924, 1088, 1116
SsiI CCGC 3 cut(s) 31, 173, 567
SspMI CTAG 4 cut(s) 534, 789, 1022, 1190
StyD4I CCNGG 1 cut(s) 560
StyI CCWWGG 1 cut(s) 118
TaaI ACNGT 3 cut(s) 355, 493, 956
TaiI ACGT 4 cut(s) 231, 748, 819, 1186
TaqI TCGA 8 cut(s) 42, 80, 289, 358, 720, 1102, 1156, 1170
TaqII GACCGA 2 cut(s) 105, 258
TasI AATT 7 cut(s) 62, 401, 663, 690, 924, 1088, 1116
TatI WGTACW 1 cut(s) 897
TauI GCSGC 2 cut(s) 176, 569
TfiI GAWTC 7 cut(s) 19, 304, 371, 427, 449, 474, 1002
Tru1I TTAA 5 cut(s) 231, 927, 974, 1080, 1097
Tru9I TTAA 5 cut(s) 231, 927, 974, 1080, 1097
TscAI CASTG 3 cut(s) 343, 544, 961
TseFI GTSAC 1 cut(s) 185
TseI GCWGC 2 cut(s) 163, 670
Tsp45I GTSAC 1 cut(s) 185
TspDTI ATGAA 8 cut(s) 17, 408, 429, 797, 812, 885, 933, 994
TspGWI ACGGA 1 cut(s) 413
TspRI CASTG 3 cut(s) 343, 544, 961
VneI GTGCAC 1 cut(s) 1264
XagI CCTNNNNNAGG 1 cut(s) 1193
XapI RAATTY 3 cut(s) 62, 1088, 1116
XceI RCATGY 2 cut(s) 49, 548
XhoI CTCGAG 1 cut(s) 719
XspI CTAG 4 cut(s) 534, 789, 1022, 1190
ZrmI AGTACT 1 cut(s) 899
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.