Rh5DG086300

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0017270
Physical Location & Seq
Forward (+)
7583438 .. 7591178
7741 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG086300.1

Sequence Viewer

Length: 1059 bp
ATGAAGACGATGGGTGAGATTCTCCATGCCCACGACGACTTCGACATGCAACTGATAGGGAAATTCTTGAGCTTCGCTTCGAGAGGCGACCGAGTTGGACTGAACCAGATGTTGATCCAAGGCACATCTCCTGATGTGCAAGACTACGATAGTAGGACGGCGCTGCATCTGGCGGCGAGTGAGGGTCACACTCCCATTGTTGAGCTTCTCGTGTGCTACAAGGCCAACGTTAATCTCCAAGACCGATGGAAAAGAACTCCCCTGACAGATGCAAGACTCTATGGACATCGAGATATATGCAGGATTCTTGAAGTGAATGGAGCCAATGACTTTACCAGTGATCAGTTGATGACCGTTCGACACGAACAAGATTCAAATGATGTGAAGTTTGACATGACGGAATTAGATACAGATAGTTCATCAGCTATTCAACAGTTTCTTGGTTCAATTGTGCAGCAAGAGGAGATGGTTCTAATTACCGAGTATCTACCAAAAGGCAATCTTGAGGATATTTTGACGCAAAGAACACGTCTTGATGTGCCTACGGCTCTACGCTATGCACTTGATATTGCTAGGGGAATGAACTATCTTCATAAGCACGTGCCATCCCCAATAGTTCACAGCCATTTGAGTACCAGAAACTTGTTTCAGGATGAAGGTGGGCACTTGAAGATTGGGGAGTATTGGGTTCAAATGTTGCATGAACAAATTAACCCAGATCAAAATGGAAGGCAAAACAGTGATGGGACACAGGATTTAACCAAGATAGATATTTGGAGGTTCGGATTCATATATTATCAGATGCTAGAAGGAATGCACTTCCAGGCCAACACAAACTCTGATCAACCCGAACTCGTTGGTTTTAAACCGAAATTCCATTTAAGTCGATGTTCTAATAGAATTCAAGAGTTGATAGGGAAATGCACAGGTCATCCTTCTCGAAGACCCCCATTCGAAGAAGTCATACTTATCCTAGAAGAGGAACTATTGTCTGTGGGTAGAGGTGTATGTCCAGTTTACTGCCTACAGATGGAGATGGGCAACAAGTGCACCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
ko03320 ko04360 ko04510 ko05100 ko05213 map03320 map04360 map04510 map05100 map05213
Pfam Domains
Protein Families
Ank_2 Ank_4 Ank_5 Pkinase_Tyr

Protein Analysis

352

Amino Acids

40.26

Weight (kDa)

5.58

Isoelectric Point (pI)

42.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 34 - 104 6e-13 Ankyrin repeats (3 copies)
Ank_5 PF13857 40 - 88 2.3e-06 Ankyrin repeats (many copies)
Ank PF00023 52 - 82 3.4e-08 Ankyrin repeat
Ank_4 PF13637 55 - 103 1.9e-06 Ankyrin repeats (many copies)
PK_Tyr_Ser-Thr PF07714 126 - 322 6.4e-19 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 139 - 320 4.5e-13 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017270)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g27960
malus_domestica MD00G1053900.v1.1
prunus_persica Prupe.3G150700_v2.0.a1
pyrus_communis pycom17g25570
rosa_chinensis RchiOBHm_Chr5g0011771
rosa_laevigata RLG00000031861
rosa_roxburghii Rroxscaffold_1G00064780
rosa_rugosa Rorug04G0458900
rosa_samantha Rh5AG090600 Rh5BG086100 Rh5CG099900 Rh5DG086300
rosa_wichuraiana Rw5G007910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 173
AclI AACGTT 1 cut(s) 228
AclWI GGATC 1 cut(s) 109
AcsI RAATTY 3 cut(s) 62, 872, 900
AcvI CACGTG 1 cut(s) 601
AfaI GTAC 1 cut(s) 634
AfiI CCNNNNNNNGG 2 cut(s) 979, 1030
AflIII ACRYGT 1 cut(s) 527
AgsI TTSAA 7 cut(s) 311, 375, 431, 447, 670, 692, 905
AjiI CACGTC 1 cut(s) 530
AjnI CCWGG 1 cut(s) 822
AluBI AGCT 3 cut(s) 72, 205, 425
AluI AGCT 3 cut(s) 72, 205, 425
Alw21I GWGCWC 1 cut(s) 1052
Alw44I GTGCAC 1 cut(s) 1048
AlwI GGATC 1 cut(s) 109
AoxI GGCC 2 cut(s) 222, 825
ApaLI GTGCAC 1 cut(s) 1048
ApeKI GCWGC 2 cut(s) 163, 454
ApoI RAATTY 3 cut(s) 62, 872, 900
ArsI GACNNNNNNTTYG 2 cut(s) 514, 546
AspLEI GCGC 1 cut(s) 163
AsuHPI GGTGA 1 cut(s) 26
AsuII TTCGAA 1 cut(s) 954
BaeGI GKGCMC 2 cut(s) 666, 1052
BarI GAAGNNNNNNTAC 2 cut(s) 948, 980
BauI CACGAG 1 cut(s) 209
BbrPI CACGTG 1 cut(s) 601
BbsI GAAGAC 2 cut(s) 11, 949
Bbv12I GWGCWC 1 cut(s) 1052
BbvI GCAGC 2 cut(s) 150, 466
BccI CCATC 7 cut(s) 4, 240, 460, 613, 737, 1024, 1030
BceAI ACGGC 2 cut(s) 174, 561
BcgI CGANNNNNNTGC 2 cut(s) 279, 313
BciT130I CCWGG 1 cut(s) 824
BclI TGATCA 2 cut(s) 340, 841
BfaI CTAG 3 cut(s) 573, 806, 974
BfmI CTRYAG 1 cut(s) 1025
BfoI RGCGCY 1 cut(s) 164
BisI GCNGC 3 cut(s) 164, 174, 455
BlsI GCNGC 3 cut(s) 165, 175, 456
Bme1390I CCNGG 1 cut(s) 824
BmgBI CACGTC 1 cut(s) 530
BmiI GGNNCC 1 cut(s) 322
BmrFI CCNGG 1 cut(s) 824
BmsI GCATC 3 cut(s) 175, 259, 792
BpiI GAAGAC 2 cut(s) 11, 949
Bpu14I TTCGAA 1 cut(s) 954
BpuEI CTTGAG 2 cut(s) 88, 524
BsaAI YACGTR 1 cut(s) 601
BsaBI GATNNNNATC 1 cut(s) 113
BsaJI CCNNGG 1 cut(s) 118
Bsc4I CCNNNNNNNGG 2 cut(s) 979, 1030
Bse1I ACTGG 2 cut(s) 336, 1013
Bse8I GATNNNNATC 1 cut(s) 113
BseBI CCWGG 1 cut(s) 824
BseDI CCNNGG 1 cut(s) 118
BseGI GGATG 3 cut(s) 605, 658, 931
BseJI GATNNNNATC 1 cut(s) 113
BseLI CCNNNNNNNGG 2 cut(s) 979, 1030
BseNI ACTGG 2 cut(s) 336, 1013
BseRI GAGGAG 1 cut(s) 476
BseSI GKGCMC 2 cut(s) 666, 1052
BseXI GCAGC 2 cut(s) 150, 466
BsgI GTGCAG 1 cut(s) 473
Bsh1285I CGRYCG 1 cut(s) 91
BshFI GGCC 2 cut(s) 224, 827
BsiEI CGRYCG 1 cut(s) 91
BsiHKAI GWGCWC 1 cut(s) 1052
BslFI GGGAC 1 cut(s) 760
BslI CCNNNNNNNGG 2 cut(s) 979, 1030
BsmFI GGGAC 1 cut(s) 760
BsmI GAATGC 1 cut(s) 819
BsnI GGCC 2 cut(s) 224, 827
Bsp119I TTCGAA 1 cut(s) 954
Bsp1286I GDGCHC 2 cut(s) 666, 1052
Bsp143I GATC 4 cut(s) 114, 340, 718, 841
BspACI CCGC 1 cut(s) 173
BspANI GGCC 2 cut(s) 224, 827
BspLI GGNNCC 1 cut(s) 322
BspPI GGATC 1 cut(s) 109
BspT104I TTCGAA 1 cut(s) 954
BsrI ACTGG 2 cut(s) 336, 1013
BssECI CCNNGG 1 cut(s) 118
BssMI GATC 4 cut(s) 114, 340, 718, 841
BssSI CACGAG 1 cut(s) 209
BssT1I CCWWGG 1 cut(s) 118
Bst2BI CACGAG 1 cut(s) 209
Bst2UI CCWGG 1 cut(s) 824
Bst4CI ACNGT 3 cut(s) 355, 435, 740
Bst6I CTCTTC 1 cut(s) 972
BstAPI GCANNNNNTGC 1 cut(s) 1047
BstBAI YACGTR 1 cut(s) 601
BstBI TTCGAA 1 cut(s) 954
BstENI CCTNNNNNAGG 1 cut(s) 977
BstF5I GGATG 3 cut(s) 605, 658, 931
BstH2I RGCGCY 1 cut(s) 164
BstHHI GCGC 1 cut(s) 163
BstKTI GATC 4 cut(s) 117, 343, 721, 844
BstMBI GATC 4 cut(s) 114, 340, 718, 841
BstMCI CGRYCG 1 cut(s) 91
BstMWI GCNNNNNNNGC 1 cut(s) 1047
BstNI CCWGG 1 cut(s) 824
BstNSI RCATGY 1 cut(s) 49
BstSCI CCNGG 1 cut(s) 822
BstSFI CTRYAG 1 cut(s) 1025
BstSLI GKGCMC 2 cut(s) 666, 1052
BstV1I GCAGC 2 cut(s) 150, 466
BstV2I GAAGAC 2 cut(s) 11, 949
BsuRI GGCC 2 cut(s) 224, 827
BtrI CACGTC 1 cut(s) 530
BtsCI GGATG 3 cut(s) 605, 658, 931
BtsIMutI CAGTG 2 cut(s) 343, 745
CfoI GCGC 1 cut(s) 163
CseI GACGC 1 cut(s) 526
Csp6I GTAC 1 cut(s) 633
CviAII CATG 4 cut(s) 26, 46, 394, 701
CviJI RGCY 8 cut(s) 72, 205, 224, 323, 425, 548, 624, 827
CviKI_1 RGCY 8 cut(s) 72, 205, 224, 323, 425, 548, 624, 827
CviQI GTAC 1 cut(s) 633
DpnI GATC 4 cut(s) 116, 342, 720, 843
DpnII GATC 4 cut(s) 114, 340, 718, 841
DraI TTTAAA 1 cut(s) 865
Eam1104I CTCTTC 1 cut(s) 972
EarI CTCTTC 1 cut(s) 972
Eco130I CCWWGG 1 cut(s) 118
Eco72I CACGTG 1 cut(s) 601
EcoNI CCTNNNNNAGG 1 cut(s) 977
EcoRI GAATTC 1 cut(s) 900
EcoRII CCWGG 1 cut(s) 822
EcoT14I CCWWGG 1 cut(s) 118
ErhI CCWWGG 1 cut(s) 118
FaeI CATG 4 cut(s) 29, 49, 397, 704
FalI AAGNNNNNCTT 4 cut(s) 486, 518, 951, 983
FaqI GGGAC 1 cut(s) 760
FatI CATG 4 cut(s) 25, 45, 393, 700
FbaI TGATCA 2 cut(s) 340, 841
Fnu4HI GCNGC 3 cut(s) 164, 174, 455
FokI GGATG 3 cut(s) 592, 665, 918
Fsp4HI GCNGC 3 cut(s) 164, 174, 455
FspBI CTAG 3 cut(s) 573, 806, 974
GlaI GCGC 1 cut(s) 162
GluI GCNGC 3 cut(s) 164, 174, 455
HaeII RGCGCY 1 cut(s) 164
HaeIII GGCC 2 cut(s) 224, 827
HgaI GACGC 1 cut(s) 526
HhaI GCGC 1 cut(s) 163
Hin1II CATG 4 cut(s) 29, 49, 397, 704
Hin6I GCGC 1 cut(s) 161
HinP1I GCGC 1 cut(s) 161
HinfI GANTC 5 cut(s) 19, 276, 304, 371, 786
HphI GGTGA 1 cut(s) 26
Hpy166II GTNNAC 3 cut(s) 619, 1018, 1050
Hpy188I TCNGA 3 cut(s) 785, 801, 841
Hpy8I GTNNAC 3 cut(s) 619, 1018, 1050
Hpy99I CGWCG 1 cut(s) 38
HpyAV CCTTC 4 cut(s) 650, 723, 803, 945
HpyCH4III ACNGT 3 cut(s) 355, 435, 740
HpyCH4IV ACGT 3 cut(s) 228, 529, 600
HpyF10VI GCNNNNNNNGC 1 cut(s) 1047
HpySE526I ACGT 3 cut(s) 228, 529, 600
Hsp92II CATG 4 cut(s) 29, 49, 397, 704
HspAI GCGC 1 cut(s) 161
Ksp22I TGATCA 2 cut(s) 340, 841
Kzo9I GATC 4 cut(s) 114, 340, 718, 841
LmnI GCTCC 1 cut(s) 320
Lsp1109I GCAGC 2 cut(s) 150, 466
LweI GCATC 3 cut(s) 175, 259, 792
MaeI CTAG 3 cut(s) 573, 806, 974
MaeII ACGT 3 cut(s) 228, 529, 600
MaeIII GTNAC 1 cut(s) 185
MalI GATC 4 cut(s) 116, 342, 720, 843
MboI GATC 4 cut(s) 114, 340, 718, 841
MboII GAAGA 6 cut(s) 16, 581, 682, 954, 968, 989
MfeI CAATTG 1 cut(s) 447
MhlI GDGCHC 2 cut(s) 666, 1052
MluCI AATT 7 cut(s) 62, 401, 447, 474, 708, 872, 900
MlyI GAGTC 1 cut(s) 270
MmeI TCCRAC 1 cut(s) 76
MnlI CCTC 7 cut(s) 77, 175, 454, 499, 771, 973, 995
MseI TTAA 5 cut(s) 231, 711, 758, 864, 881
MspR9I CCNGG 1 cut(s) 824
MunI CAATTG 1 cut(s) 447
Mva1269I GAATGC 1 cut(s) 819
MvaI CCWGG 1 cut(s) 824
MwoI GCNNNNNNNGC 1 cut(s) 1047
NdeII GATC 4 cut(s) 114, 340, 718, 841
NlaIII CATG 4 cut(s) 29, 49, 397, 704
NlaIV GGNNCC 1 cut(s) 322
NmuCI GTSAC 1 cut(s) 185
NspI RCATGY 1 cut(s) 49
NspV TTCGAA 1 cut(s) 954
PcsI WCGNNNNNNNCGW 1 cut(s) 39
PctI GAATGC 1 cut(s) 819
PfeI GAWTC 4 cut(s) 19, 304, 371, 786
PkrI GCNGC 3 cut(s) 165, 175, 456
PleI GAGTC 1 cut(s) 270
PmaCI CACGTG 1 cut(s) 601
PmlI CACGTG 1 cut(s) 601
PpsI GAGTC 1 cut(s) 270
Ppu21I YACGTR 1 cut(s) 601
Psp1406I AACGTT 1 cut(s) 228
Psp6I CCWGG 1 cut(s) 822
PspCI CACGTG 1 cut(s) 601
PspGI CCWGG 1 cut(s) 822
PspN4I GGNNCC 1 cut(s) 322
RsaI GTAC 1 cut(s) 634
RsaNI GTAC 1 cut(s) 633
SaqAI TTAA 5 cut(s) 231, 711, 758, 864, 881
SatI GCNGC 3 cut(s) 164, 174, 455
Sau3AI GATC 4 cut(s) 114, 340, 718, 841
SchI GAGTC 1 cut(s) 270
ScrFI CCNGG 1 cut(s) 824
SduI GDGCHC 2 cut(s) 666, 1052
SfaNI GCATC 3 cut(s) 175, 259, 792
SfcI CTRYAG 1 cut(s) 1025
SfuI TTCGAA 1 cut(s) 954
SmlI CTYRAG 2 cut(s) 67, 503
SmoI CTYRAG 2 cut(s) 67, 503
Sse9I AATT 7 cut(s) 62, 401, 447, 474, 708, 872, 900
SsiI CCGC 1 cut(s) 173
SspMI CTAG 3 cut(s) 573, 806, 974
StyD4I CCNGG 1 cut(s) 822
StyI CCWWGG 1 cut(s) 118
TaaI ACNGT 3 cut(s) 355, 435, 740
TaiI ACGT 3 cut(s) 231, 532, 603
TaqI TCGA 7 cut(s) 42, 80, 289, 358, 886, 940, 954
TaqII GACCGA 2 cut(s) 105, 258
TasI AATT 7 cut(s) 62, 401, 447, 474, 708, 872, 900
TauI GCSGC 1 cut(s) 176
TfiI GAWTC 4 cut(s) 19, 304, 371, 786
Tru1I TTAA 5 cut(s) 231, 711, 758, 864, 881
Tru9I TTAA 5 cut(s) 231, 711, 758, 864, 881
TscAI CASTG 2 cut(s) 343, 745
TseFI GTSAC 1 cut(s) 185
TseI GCWGC 2 cut(s) 163, 454
Tsp45I GTSAC 1 cut(s) 185
TspDTI ATGAA 7 cut(s) 17, 408, 581, 596, 669, 717, 778
TspGWI ACGGA 1 cut(s) 413
TspRI CASTG 2 cut(s) 343, 745
VneI GTGCAC 1 cut(s) 1048
XagI CCTNNNNNAGG 1 cut(s) 977
XapI RAATTY 3 cut(s) 62, 872, 900
XceI RCATGY 1 cut(s) 49
XspI CTAG 3 cut(s) 573, 806, 974
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.