Rw5G007910

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0017270
Physical Location & Seq
Forward (+)
8429871 .. 8435793
5923 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G007910.1

Sequence Viewer

Length: 1248 bp
ATGAAGACGATGGGTGAGATTCTCCATGCCCGCGACGACTTCGACATGCAACAGATAGGGAAATTCTTGAGCTTCGCTTCGAGAGGCGACCGAGTTGGACTGAACCAGATGTTGATCCAAGGCACATCTCCTGATGTGCAAGACTACGATAATAGGACGGCGCTGCATCTGGCGGCGAGTGAGGGTCACACTCCCATTGTTGAGCTTCTCGTCTGCTACAAGGCCAACGTTAATCTCCAAGACCGATGGAAAAGAACTCCCCTGACAGATGCAAGACTCTATGGACATCGAGATATATGCAGGATTCTTGAAGTGAATGGAGCCAATGACTTTACCAGTGATCAGTTGATGACCGTTCGACACGAACAAGATTCAAATGATGTGAAGTTTGACATGACGGAATTAGTTACAGATAGTTCATCAGCGATTCAACAGGGTTCATTTGGTGAATCTGAAAAGGTGAAGTGGCGTGGAATCTGGGTCGTAAAGACCGTTGTCAAAAAAGACATATGTGCTCATGCTTGGCGACAGCCCAGGACCGCAGAGCAAATGGGGCACCAAATGATACTCTCTGCTAAGGATAATACTCTCTTACGGGAGCTTAGACATCCCAATATATTGCAGTTTCTTGGTTCAATTGTGCAGCAAGAGGAGATGGTTCTAATTACCGAGTATCTACCAAAAGGCAATCTCGAGGATATTTTGACGCAAAGAACACGTCTTGATGTGCCTACGGCTCTACGCTATGCACTTGATATTGCTAGGGGAATGAACTATCTTCATAAGCACGTGCCATCCCCAATAGTTCACAGCCATTTGAGTACCAGAAACTTGTTTCAGGATGAAGGTGGGCACTTGAAGATTGGGGAGTATTGGGTTCAAATGTTGCATGAACAAATTAACCCAGATCAAAATGGAAGGCAAAACAGTGATGGGACGCAGGATTTAACCAAGATAGATATTTGGAGGTTCGGATTCATATATTATCAGATGCTAGAAGGAATGCACTTCCAGGCCAACACAAACTCTGATCAACCCGAACTCGTTGATTTTAAACCGAAATTCCATTTAAGTCGGTGTTCTAATAGAATTCAAGAGTTGATAGGGAAATGCACAGGTCATCCTTCTCGAAGACCCCCATTCGAAGAAGTCATACTTATCCTAGAAGAGGAACTATTGTCTGTGGGTAGAGGTGTATGTCCAGTTTACTGCCTACAGATGGAGATGGGCAACAAGTGCACCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
ko03320 ko04360 ko04510 ko05100 ko05213 map03320 map04360 map04510 map05100 map05213
Pfam Domains
Protein Families
Ank_2 Ank_4 Ank_5 Pkinase_Tyr

Protein Analysis

415

Amino Acids

47.67

Weight (kDa)

6.19

Isoelectric Point (pI)

41.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 34 - 104 4.4e-13 Ankyrin repeats (3 copies)
Ank_5 PF13857 39 - 88 1.8e-06 Ankyrin repeats (many copies)
Ank PF00023 50 - 82 1.9e-08 Ankyrin repeat
Ank_4 PF13637 55 - 103 2.4e-06 Ankyrin repeats (many copies)
PK_Tyr_Ser-Thr PF07714 141 - 385 5.8e-25 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 189 - 383 6.2e-18 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017270)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g27960
malus_domestica MD00G1053900.v1.1
prunus_persica Prupe.3G150700_v2.0.a1
pyrus_communis pycom17g25570
rosa_chinensis RchiOBHm_Chr5g0011771
rosa_laevigata RLG00000031861
rosa_roxburghii Rroxscaffold_1G00064780
rosa_rugosa Rorug04G0458900
rosa_samantha Rh5AG090600 Rh5BG086100 Rh5CG099900 Rh5DG086300
rosa_wichuraiana Rw5G007910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 555
AccII CGCG 1 cut(s) 33
AciI CCGC 3 cut(s) 31, 173, 540
AclI AACGTT 1 cut(s) 228
AclWI GGATC 1 cut(s) 109
AcsI RAATTY 3 cut(s) 62, 1061, 1089
AcvI CACGTG 1 cut(s) 790
AfaI GTAC 1 cut(s) 823
AfiI CCNNNNNNNGG 2 cut(s) 1168, 1219
AflIII ACRYGT 1 cut(s) 716
AgsI TTSAA 7 cut(s) 311, 375, 431, 636, 859, 881, 1094
AjiI CACGTC 1 cut(s) 719
AjnI CCWGG 2 cut(s) 533, 1011
AluBI AGCT 3 cut(s) 72, 205, 601
AluI AGCT 3 cut(s) 72, 205, 601
Alw21I GWGCWC 2 cut(s) 517, 1241
Alw44I GTGCAC 1 cut(s) 1237
AlwI GGATC 1 cut(s) 109
Ama87I CYCGRG 1 cut(s) 692
AoxI GGCC 2 cut(s) 222, 1014
ApaLI GTGCAC 1 cut(s) 1237
ApeKI GCWGC 2 cut(s) 163, 643
ApoI RAATTY 3 cut(s) 62, 1061, 1089
ArsI GACNNNNNNTTYG 2 cut(s) 703, 735
AspLEI GCGC 1 cut(s) 163
AspS9I GGNCC 1 cut(s) 537
AsuHPI GGTGA 3 cut(s) 26, 458, 472
AsuII TTCGAA 1 cut(s) 1143
AvaI CYCGRG 1 cut(s) 692
AvaII GGWCC 1 cut(s) 537
BaeGI GKGCMC 3 cut(s) 558, 855, 1241
BanI GGYRCC 1 cut(s) 555
BarI GAAGNNNNNNTAC 2 cut(s) 1137, 1169
BbrPI CACGTG 1 cut(s) 790
BbsI GAAGAC 2 cut(s) 11, 1138
Bbv12I GWGCWC 2 cut(s) 517, 1241
BbvI GCAGC 2 cut(s) 150, 655
BccI CCATC 7 cut(s) 4, 240, 649, 802, 926, 1213, 1219
BceAI ACGGC 2 cut(s) 174, 750
BcgI CGANNNNNNTGC 2 cut(s) 279, 313
BciT130I CCWGG 2 cut(s) 535, 1013
BclI TGATCA 2 cut(s) 340, 1030
BfaI CTAG 3 cut(s) 762, 995, 1163
BfmI CTRYAG 1 cut(s) 1214
BfoI RGCGCY 1 cut(s) 164
BisI GCNGC 3 cut(s) 164, 174, 644
BlsI GCNGC 3 cut(s) 165, 175, 645
Bme1390I CCNGG 2 cut(s) 535, 1013
Bme18I GGWCC 1 cut(s) 537
BmeT110I CYCGRG 1 cut(s) 692
BmgBI CACGTC 1 cut(s) 719
BmgT120I GGNCC 1 cut(s) 537
BmiI GGNNCC 2 cut(s) 322, 557
BmrFI CCNGG 2 cut(s) 535, 1013
BmsI GCATC 3 cut(s) 175, 259, 981
BoxI GACNNNNGTC 1 cut(s) 494
BpiI GAAGAC 2 cut(s) 11, 1138
Bpu10I CCTNAGC 1 cut(s) 576
Bpu14I TTCGAA 1 cut(s) 1143
BpuEI CTTGAG 1 cut(s) 88
BsaAI YACGTR 1 cut(s) 790
BsaBI GATNNNNATC 1 cut(s) 113
BsaJI CCNNGG 2 cut(s) 118, 533
Bsc4I CCNNNNNNNGG 2 cut(s) 1168, 1219
Bse1I ACTGG 2 cut(s) 336, 1202
Bse8I GATNNNNATC 1 cut(s) 113
BseBI CCWGG 2 cut(s) 535, 1013
BseDI CCNNGG 2 cut(s) 118, 533
BseGI GGATG 4 cut(s) 607, 794, 847, 1120
BseJI GATNNNNATC 1 cut(s) 113
BseLI CCNNNNNNNGG 2 cut(s) 1168, 1219
BseNI ACTGG 2 cut(s) 336, 1202
BseRI GAGGAG 1 cut(s) 665
BseSI GKGCMC 3 cut(s) 558, 855, 1241
BseXI GCAGC 2 cut(s) 150, 655
BsgI GTGCAG 1 cut(s) 662
Bsh1236I CGCG 1 cut(s) 33
Bsh1285I CGRYCG 1 cut(s) 91
BshFI GGCC 2 cut(s) 224, 1016
BshNI GGYRCC 1 cut(s) 555
BsiEI CGRYCG 1 cut(s) 91
BsiHKAI GWGCWC 2 cut(s) 517, 1241
BsiHKCI CYCGRG 1 cut(s) 692
BslFI GGGAC 1 cut(s) 949
BslI CCNNNNNNNGG 2 cut(s) 1168, 1219
BsmFI GGGAC 1 cut(s) 949
BsmI GAATGC 1 cut(s) 1008
BsnI GGCC 2 cut(s) 224, 1016
BsoBI CYCGRG 1 cut(s) 692
Bsp119I TTCGAA 1 cut(s) 1143
Bsp1286I GDGCHC 4 cut(s) 517, 558, 855, 1241
Bsp143I GATC 4 cut(s) 114, 340, 907, 1030
BspACI CCGC 3 cut(s) 31, 173, 540
BspANI GGCC 2 cut(s) 224, 1016
BspFNI CGCG 1 cut(s) 33
BspLI GGNNCC 2 cut(s) 322, 557
BspPI GGATC 1 cut(s) 109
BspT104I TTCGAA 1 cut(s) 1143
BspT107I GGYRCC 1 cut(s) 555
BsrI ACTGG 2 cut(s) 336, 1202
BssECI CCNNGG 2 cut(s) 118, 533
BssMI GATC 4 cut(s) 114, 340, 907, 1030
BssT1I CCWWGG 1 cut(s) 118
Bst2UI CCWGG 2 cut(s) 535, 1013
Bst4CI ACNGT 3 cut(s) 355, 493, 929
Bst6I CTCTTC 1 cut(s) 1161
BstAPI GCANNNNNTGC 1 cut(s) 1236
BstBAI YACGTR 1 cut(s) 790
BstBI TTCGAA 1 cut(s) 1143
BstC8I GCNNGC 1 cut(s) 31
BstDEI CTNAG 2 cut(s) 576, 602
BstENI CCTNNNNNAGG 1 cut(s) 1166
BstF5I GGATG 4 cut(s) 607, 794, 847, 1120
BstFNI CGCG 1 cut(s) 33
BstH2I RGCGCY 1 cut(s) 164
BstHHI GCGC 1 cut(s) 163
BstKTI GATC 4 cut(s) 117, 343, 910, 1033
BstMBI GATC 4 cut(s) 114, 340, 907, 1030
BstMCI CGRYCG 1 cut(s) 91
BstMWI GCNNNNNNNGC 2 cut(s) 553, 1236
BstNI CCWGG 2 cut(s) 535, 1013
BstNSI RCATGY 1 cut(s) 49
BstPAI GACNNNNGTC 1 cut(s) 494
BstSCI CCNGG 2 cut(s) 533, 1011
BstSFI CTRYAG 1 cut(s) 1214
BstSLI GKGCMC 3 cut(s) 558, 855, 1241
BstUI CGCG 1 cut(s) 33
BstV1I GCAGC 2 cut(s) 150, 655
BstV2I GAAGAC 2 cut(s) 11, 1138
BsuRI GGCC 2 cut(s) 224, 1016
BtrI CACGTC 1 cut(s) 719
BtsCI GGATG 4 cut(s) 607, 794, 847, 1120
BtsIMutI CAGTG 2 cut(s) 343, 934
Cac8I GCNNGC 1 cut(s) 31
CfoI GCGC 1 cut(s) 163
Cfr13I GGNCC 1 cut(s) 537
CseI GACGC 2 cut(s) 715, 946
Csp6I GTAC 1 cut(s) 822
CviAII CATG 5 cut(s) 26, 46, 394, 518, 890
CviJI RGCY 9 cut(s) 72, 205, 224, 323, 532, 601, 737, 813, 1016
CviKI_1 RGCY 9 cut(s) 72, 205, 224, 323, 532, 601, 737, 813, 1016
CviQI GTAC 1 cut(s) 822
DdeI CTNAG 2 cut(s) 576, 602
DpnI GATC 4 cut(s) 116, 342, 909, 1032
DpnII GATC 4 cut(s) 114, 340, 907, 1030
DraI TTTAAA 1 cut(s) 1054
Eam1104I CTCTTC 1 cut(s) 1161
EarI CTCTTC 1 cut(s) 1161
Eco130I CCWWGG 1 cut(s) 118
Eco47I GGWCC 1 cut(s) 537
Eco72I CACGTG 1 cut(s) 790
Eco88I CYCGRG 1 cut(s) 692
EcoNI CCTNNNNNAGG 1 cut(s) 1166
EcoRI GAATTC 1 cut(s) 1089
EcoRII CCWGG 2 cut(s) 533, 1011
EcoT14I CCWWGG 1 cut(s) 118
ErhI CCWWGG 1 cut(s) 118
FaeI CATG 5 cut(s) 29, 49, 397, 521, 893
FalI AAGNNNNNCTT 2 cut(s) 1140, 1172
FaqI GGGAC 1 cut(s) 949
FatI CATG 5 cut(s) 25, 45, 393, 517, 889
FauI CCCGC 1 cut(s) 38
FauNDI CATATG 1 cut(s) 509
FbaI TGATCA 2 cut(s) 340, 1030
Fnu4HI GCNGC 3 cut(s) 164, 174, 644
FokI GGATG 4 cut(s) 594, 781, 854, 1107
Fsp4HI GCNGC 3 cut(s) 164, 174, 644
FspBI CTAG 3 cut(s) 762, 995, 1163
GlaI GCGC 1 cut(s) 162
GluI GCNGC 3 cut(s) 164, 174, 644
HaeII RGCGCY 1 cut(s) 164
HaeIII GGCC 2 cut(s) 224, 1016
HgaI GACGC 2 cut(s) 715, 946
HhaI GCGC 1 cut(s) 163
Hin1II CATG 5 cut(s) 29, 49, 397, 521, 893
Hin6I GCGC 1 cut(s) 161
HinP1I GCGC 1 cut(s) 161
HinfI GANTC 8 cut(s) 19, 276, 304, 371, 427, 449, 474, 975
HphI GGTGA 3 cut(s) 26, 458, 472
Hpy166II GTNNAC 3 cut(s) 808, 1207, 1239
Hpy188I TCNGA 4 cut(s) 454, 974, 990, 1030
Hpy8I GTNNAC 3 cut(s) 808, 1207, 1239
Hpy99I CGWCG 1 cut(s) 38
HpyAV CCTTC 4 cut(s) 839, 912, 992, 1134
HpyCH4III ACNGT 3 cut(s) 355, 493, 929
HpyCH4IV ACGT 3 cut(s) 228, 718, 789
HpyF10VI GCNNNNNNNGC 2 cut(s) 553, 1236
HpyF3I CTNAG 2 cut(s) 576, 602
HpySE526I ACGT 3 cut(s) 228, 718, 789
Hsp92II CATG 5 cut(s) 29, 49, 397, 521, 893
HspAI GCGC 1 cut(s) 161
Ksp22I TGATCA 2 cut(s) 340, 1030
Kzo9I GATC 4 cut(s) 114, 340, 907, 1030
LmnI GCTCC 2 cut(s) 320, 598
Lsp1109I GCAGC 2 cut(s) 150, 655
LweI GCATC 3 cut(s) 175, 259, 981
MaeI CTAG 3 cut(s) 762, 995, 1163
MaeII ACGT 3 cut(s) 228, 718, 789
MaeIII GTNAC 2 cut(s) 185, 406
MalI GATC 4 cut(s) 116, 342, 909, 1032
MboI GATC 4 cut(s) 114, 340, 907, 1030
MboII GAAGA 6 cut(s) 16, 770, 871, 1143, 1157, 1178
MfeI CAATTG 1 cut(s) 636
MhlI GDGCHC 4 cut(s) 517, 558, 855, 1241
MluCI AATT 7 cut(s) 62, 401, 636, 663, 897, 1061, 1089
MlyI GAGTC 1 cut(s) 270
MmeI TCCRAC 1 cut(s) 76
MnlI CCTC 7 cut(s) 77, 175, 643, 688, 960, 1162, 1184
MseI TTAA 5 cut(s) 231, 900, 947, 1053, 1070
MspR9I CCNGG 2 cut(s) 535, 1013
MunI CAATTG 1 cut(s) 636
Mva1269I GAATGC 1 cut(s) 1008
MvaI CCWGG 2 cut(s) 535, 1013
MvnI CGCG 1 cut(s) 33
MwoI GCNNNNNNNGC 2 cut(s) 553, 1236
NdeI CATATG 1 cut(s) 509
NdeII GATC 4 cut(s) 114, 340, 907, 1030
NlaIII CATG 5 cut(s) 29, 49, 397, 521, 893
NlaIV GGNNCC 2 cut(s) 322, 557
NmuCI GTSAC 1 cut(s) 185
NspI RCATGY 1 cut(s) 49
NspV TTCGAA 1 cut(s) 1143
PaeR7I CTCGAG 1 cut(s) 692
PcsI WCGNNNNNNNCGW 1 cut(s) 489
PctI GAATGC 1 cut(s) 1008
PfeI GAWTC 7 cut(s) 19, 304, 371, 427, 449, 474, 975
PkrI GCNGC 3 cut(s) 165, 175, 645
PleI GAGTC 1 cut(s) 270
PmaCI CACGTG 1 cut(s) 790
PmlI CACGTG 1 cut(s) 790
PpsI GAGTC 1 cut(s) 270
Ppu21I YACGTR 1 cut(s) 790
PshAI GACNNNNGTC 1 cut(s) 494
Psp1406I AACGTT 1 cut(s) 228
Psp6I CCWGG 2 cut(s) 533, 1011
PspCI CACGTG 1 cut(s) 790
PspGI CCWGG 2 cut(s) 533, 1011
PspN4I GGNNCC 2 cut(s) 322, 557
PspPI GGNCC 1 cut(s) 537
RsaI GTAC 1 cut(s) 823
RsaNI GTAC 1 cut(s) 822
SaqAI TTAA 5 cut(s) 231, 900, 947, 1053, 1070
SatI GCNGC 3 cut(s) 164, 174, 644
Sau3AI GATC 4 cut(s) 114, 340, 907, 1030
Sau96I GGNCC 1 cut(s) 537
SchI GAGTC 1 cut(s) 270
ScrFI CCNGG 2 cut(s) 535, 1013
SduI GDGCHC 4 cut(s) 517, 558, 855, 1241
SfaNI GCATC 3 cut(s) 175, 259, 981
SfcI CTRYAG 1 cut(s) 1214
Sfr274I CTCGAG 1 cut(s) 692
SfuI TTCGAA 1 cut(s) 1143
SinI GGWCC 1 cut(s) 537
SlaI CTCGAG 1 cut(s) 692
SmlI CTYRAG 2 cut(s) 67, 692
SmoI CTYRAG 2 cut(s) 67, 692
Sse9I AATT 7 cut(s) 62, 401, 636, 663, 897, 1061, 1089
SsiI CCGC 3 cut(s) 31, 173, 540
SspMI CTAG 3 cut(s) 762, 995, 1163
StyD4I CCNGG 2 cut(s) 533, 1011
StyI CCWWGG 1 cut(s) 118
TaaI ACNGT 3 cut(s) 355, 493, 929
TaiI ACGT 3 cut(s) 231, 721, 792
TaqI TCGA 7 cut(s) 42, 80, 289, 358, 693, 1129, 1143
TaqII GACCGA 2 cut(s) 105, 258
TasI AATT 7 cut(s) 62, 401, 636, 663, 897, 1061, 1089
TauI GCSGC 1 cut(s) 176
TfiI GAWTC 7 cut(s) 19, 304, 371, 427, 449, 474, 975
Tru1I TTAA 5 cut(s) 231, 900, 947, 1053, 1070
Tru9I TTAA 5 cut(s) 231, 900, 947, 1053, 1070
TscAI CASTG 2 cut(s) 343, 934
TseFI GTSAC 1 cut(s) 185
TseI GCWGC 2 cut(s) 163, 643
Tsp45I GTSAC 1 cut(s) 185
TspDTI ATGAA 8 cut(s) 17, 408, 429, 770, 785, 858, 906, 967
TspGWI ACGGA 1 cut(s) 413
TspRI CASTG 2 cut(s) 343, 934
VneI GTGCAC 1 cut(s) 1237
VpaK11BI GGWCC 1 cut(s) 537
XagI CCTNNNNNAGG 1 cut(s) 1166
XapI RAATTY 3 cut(s) 62, 1061, 1089
XceI RCATGY 1 cut(s) 49
XhoI CTCGAG 1 cut(s) 692
XspI CTAG 3 cut(s) 762, 995, 1163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.