MD00G1065500.v1.1

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0018098
Physical Location & Seq
Reverse (-)
12613068 .. 12614012
945 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1065500.v1.1.491

Sequence Viewer

Length: 945 bp
ATGTCAATACCCTTCACCCTTATTGATGCAACCCCTGACTCATACTCAAGGTTCATAGATCAACTGCGAGCTCGACTCACATTCGGCACCACATCTCAGGGTATACGGGTGCTGCCTCCAAGTCGACAAGTCGGGAACAACGCCCGGTTTATTTATGTTGATCTCACCAACTATGACGGAGTCACTGTCACAATCGGAATTGATGTTGTGAACGCTTACGTGATGGGTTACGAGCAAGGTGAGCAAAACTACCCTCTTCAAACTCTTCCTGATGACCCAGCTCCAGTCGAACTGTTATTCCCCAACACCAGGTCAGCTGGTGAACTGCCCTTCACAGGCCACTACGCCTCGCTTGGGGAATATGCACGACGTATGCAGAATGAACAACCTAATCGTCGTGACCAACAAGCACTTAACCGACTGAGTAATCCTATGCGTCAGAATATCGGTCTGGGACCCAGCTCCCTTCATAGCGCCATTGACATGTTGGAGCGCGCAGCAACGCCCCTCTCCCAAGCGGGTGCGATACTTGTGATCATTCAAATGGTCTCGGAAGCAGCAAGGTACCCATACATTGAGAGGCAAGTCCGCGAGTCCATTCAGACGGGAAATTCATTTCTACCTGACCCTCGTATGCTGAGCCTGGAGAATAATTGGAGCAATCTCTCCCGACAGATCATGGGGGCAACTCGCGCAGGTCGAGAAAGCTTCAGTACTTCTGTTTCGTTGGACGATGCCTACCAAAGTCATGGTGCTCCTCCCCTCGTAGTAAACAGTGTTCGAGATTCATTCATTCAGGACATGGAAATCGCGTTGCTGCTCCATGACCGAGGTGACGACCGAGGTACCGACCAAGGTAATGACCCAGAGAATTGCACCCCTGGACCTTCAGGTTCAGGGATAGGGAGACGGGGAGGGAAAAAGCCTCGTCGTCAGCACGAGTAA

Protein Analysis

315

Amino Acids

34.74

Weight (kDa)

5.63

Isoelectric Point (pI)

45.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RIP PF00161 11 - 226 4.1e-36 Ribosome inactivating protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 686
Acc65I GGTACC 2 cut(s) 564, 845
AccB1I GGYRCC 3 cut(s) 86, 564, 845
AccI GTMKAC 2 cut(s) 103, 124
AccII CGCG 4 cut(s) 495, 591, 693, 812
AciI CCGC 2 cut(s) 518, 589
AcsI RAATTY 1 cut(s) 610
AcuI CTGAAG 2 cut(s) 694, 873
AfaI GTAC 3 cut(s) 566, 715, 847
AfiI CCNNNNNNNGG 3 cut(s) 309, 335, 354
AflIII ACRYGT 1 cut(s) 483
AgsI TTSAA 2 cut(s) 260, 542
AjnI CCWGG 3 cut(s) 308, 642, 880
AloI GAACNNNNNNTCC 2 cut(s) 282, 314
AluBI AGCT 5 cut(s) 71, 281, 317, 462, 708
AluI AGCT 5 cut(s) 71, 281, 317, 462, 708
Alw21I GWGCWC 2 cut(s) 73, 757
Alw26I GTCTC 2 cut(s) 553, 901
AoxI GGCC 1 cut(s) 337
ApeKI GCWGC 4 cut(s) 112, 497, 557, 817
ApoI RAATTY 1 cut(s) 610
Asp718I GGTACC 2 cut(s) 564, 845
AspLEI GCGC 4 cut(s) 476, 495, 497, 695
AspS9I GGNCC 2 cut(s) 455, 884
AsuC2I CCSGG 1 cut(s) 145
AsuHPI GGTGA 5 cut(s) 7, 157, 251, 332, 845
AvaII GGWCC 2 cut(s) 455, 884
BaeI ACNNNNGTAYC 2 cut(s) 829, 862
BanI GGYRCC 3 cut(s) 86, 564, 845
BanII GRGCYC 1 cut(s) 73
BauI CACGAG 1 cut(s) 938
Bbv12I GWGCWC 2 cut(s) 73, 757
BbvI GCAGC 4 cut(s) 99, 509, 569, 804
BccI CCATC 1 cut(s) 217
BciT130I CCWGG 3 cut(s) 310, 644, 882
BclI TGATCA 1 cut(s) 534
BcnI CCSGG 1 cut(s) 145
BcoDI GTCTC 2 cut(s) 553, 901
BfoI RGCGCY 1 cut(s) 477
BfuAI ACCTGC 1 cut(s) 686
BisI GCNGC 4 cut(s) 113, 498, 558, 818
BlpI GCTNAGC 1 cut(s) 638
BlsI GCNGC 4 cut(s) 114, 499, 559, 819
BmcAI AGTACT 1 cut(s) 715
Bme1390I CCNGG 4 cut(s) 145, 310, 644, 882
Bme18I GGWCC 2 cut(s) 455, 884
BmgT120I GGNCC 2 cut(s) 455, 884
BmiI GGNNCC 5 cut(s) 88, 456, 457, 566, 847
BmrFI CCNGG 4 cut(s) 145, 310, 644, 882
BmsI GCATC 2 cut(s) 16, 724
BplI GAGNNNNNCTC 2 cut(s) 60, 92
BpmI CTGGAG 2 cut(s) 267, 665
Bpu1102I GCTNAGC 1 cut(s) 638
BpuEI CTTGAG 1 cut(s) 31
BpuMI CCSGG 1 cut(s) 145
BsaAI YACGTR 1 cut(s) 220
BsaI GGTCTC 1 cut(s) 553
BsaJI CCNNGG 4 cut(s) 829, 841, 853, 880
BsaXI ACNNNNNCTCC 2 cut(s) 171, 201
Bsc4I CCNNNNNNNGG 3 cut(s) 309, 335, 354
Bse1I ACTGG 1 cut(s) 284
BseBI CCWGG 3 cut(s) 310, 644, 882
BseDI CCNNGG 4 cut(s) 829, 841, 853, 880
BseLI CCNNNNNNNGG 3 cut(s) 309, 335, 354
BseMII CTCAG 3 cut(s) 110, 413, 629
BseNI ACTGG 1 cut(s) 284
BsePI GCGCGC 1 cut(s) 493
BseRI GAGGAG 1 cut(s) 747
BseXI GCAGC 4 cut(s) 99, 509, 569, 804
BseYI CCCAGC 2 cut(s) 277, 458
Bsh1236I CGCG 4 cut(s) 495, 591, 693, 812
Bsh1285I CGRYCG 1 cut(s) 841
BshFI GGCC 1 cut(s) 339
BshNI GGYRCC 3 cut(s) 86, 564, 845
BsiEI CGRYCG 1 cut(s) 841
BsiHKAI GWGCWC 2 cut(s) 73, 757
BsiSI CCGG 1 cut(s) 145
BslFI GGGAC 1 cut(s) 468
BslI CCNNNNNNNGG 3 cut(s) 309, 335, 354
BsmAI GTCTC 2 cut(s) 553, 901
BsmBI CGTCTC 1 cut(s) 901
BsmFI GGGAC 1 cut(s) 468
BsnI GGCC 1 cut(s) 339
Bso31I GGTCTC 1 cut(s) 553
Bsp1286I GDGCHC 2 cut(s) 73, 757
Bsp143I GATC 4 cut(s) 58, 160, 534, 675
Bsp1720I GCTNAGC 1 cut(s) 638
BspACI CCGC 2 cut(s) 518, 589
BspANI GGCC 1 cut(s) 339
BspCNI CTCAG 3 cut(s) 109, 414, 630
BspFNI CGCG 4 cut(s) 495, 591, 693, 812
BspLI GGNNCC 5 cut(s) 88, 456, 457, 566, 847
BspMI ACCTGC 1 cut(s) 686
BspT107I GGYRCC 3 cut(s) 86, 564, 845
BspTNI GGTCTC 1 cut(s) 553
BsrI ACTGG 1 cut(s) 284
BssECI CCNNGG 4 cut(s) 829, 841, 853, 880
BssHII GCGCGC 1 cut(s) 493
BssMI GATC 4 cut(s) 58, 160, 534, 675
BssNAI GTATAC 1 cut(s) 104
BssSI CACGAG 1 cut(s) 938
BssT1I CCWWGG 1 cut(s) 853
Bst1107I GTATAC 1 cut(s) 104
Bst2BI CACGAG 1 cut(s) 938
Bst2UI CCWGG 3 cut(s) 310, 644, 882
Bst4CI ACNGT 3 cut(s) 187, 294, 776
Bst6I CTCTTC 2 cut(s) 261, 270
BstBAI YACGTR 1 cut(s) 220
BstC8I GCNNGC 2 cut(s) 69, 495
BstDEI CTNAG 3 cut(s) 96, 422, 638
BstFNI CGCG 4 cut(s) 495, 591, 693, 812
BstH2I RGCGCY 1 cut(s) 477
BstHHI GCGC 4 cut(s) 476, 495, 497, 695
BstKTI GATC 4 cut(s) 61, 163, 537, 678
BstMAI GTCTC 2 cut(s) 553, 901
BstMBI GATC 4 cut(s) 58, 160, 534, 675
BstMCI CGRYCG 1 cut(s) 841
BstMWI GCNNNNNNNGC 2 cut(s) 241, 692
BstNI CCWGG 3 cut(s) 310, 644, 882
BstNSI RCATGY 1 cut(s) 487
BstSCI CCNGG 4 cut(s) 143, 308, 642, 880
BstUI CGCG 4 cut(s) 495, 591, 693, 812
BstV1I GCAGC 4 cut(s) 99, 509, 569, 804
BstXI CCANNNNNNTGG 1 cut(s) 749
BstZ17I GTATAC 1 cut(s) 104
BsuRI GGCC 1 cut(s) 339
BtsIMutI CAGTG 2 cut(s) 183, 781
BveI ACCTGC 1 cut(s) 686
Cac8I GCNNGC 2 cut(s) 69, 495
CfoI GCGC 4 cut(s) 476, 495, 497, 695
Cfr13I GGNCC 2 cut(s) 455, 884
CseI GACGC 1 cut(s) 425
CsiI ACCWGGT 1 cut(s) 308
Csp6I GTAC 3 cut(s) 565, 714, 846
CviAII CATG 5 cut(s) 484, 679, 749, 802, 824
CviJI RGCY 8 cut(s) 71, 281, 317, 339, 462, 642, 708, 925
CviKI_1 RGCY 8 cut(s) 71, 281, 317, 339, 462, 642, 708, 925
CviQI GTAC 3 cut(s) 565, 714, 846
DdeI CTNAG 3 cut(s) 96, 422, 638
DpnI GATC 4 cut(s) 60, 162, 536, 677
DpnII GATC 4 cut(s) 58, 160, 534, 675
Eam1104I CTCTTC 2 cut(s) 261, 270
EarI CTCTTC 2 cut(s) 261, 270
Ecl136II GAGCTC 1 cut(s) 71
Eco130I CCWWGG 1 cut(s) 853
Eco24I GRGCYC 1 cut(s) 73
Eco31I GGTCTC 1 cut(s) 553
Eco47I GGWCC 2 cut(s) 455, 884
Eco53kI GAGCTC 1 cut(s) 71
Eco57I CTGAAG 2 cut(s) 694, 873
EcoICRI GAGCTC 1 cut(s) 71
EcoO109I RGGNCCY 1 cut(s) 455
EcoRII CCWGG 3 cut(s) 308, 642, 880
EcoT14I CCWWGG 1 cut(s) 853
EcoT38I GRGCYC 1 cut(s) 73
ErhI CCWWGG 1 cut(s) 853
Esp3I CGTCTC 1 cut(s) 901
FaeI CATG 5 cut(s) 487, 682, 752, 805, 827
FaqI GGGAC 1 cut(s) 468
FatI CATG 5 cut(s) 483, 678, 748, 801, 823
FauI CCCGC 1 cut(s) 511
FbaI TGATCA 1 cut(s) 534
FblI GTMKAC 2 cut(s) 103, 124
Fnu4HI GCNGC 4 cut(s) 113, 498, 558, 818
FriOI GRGCYC 1 cut(s) 73
Fsp4HI GCNGC 4 cut(s) 113, 498, 558, 818
GlaI GCGC 4 cut(s) 475, 494, 496, 694
GluI GCNGC 4 cut(s) 113, 498, 558, 818
GsaI CCCAGC 2 cut(s) 281, 462
GsuI CTGGAG 2 cut(s) 267, 665
HaeII RGCGCY 1 cut(s) 477
HaeIII GGCC 1 cut(s) 339
HapII CCGG 1 cut(s) 145
HgaI GACGC 1 cut(s) 425
HhaI GCGC 4 cut(s) 476, 495, 497, 695
Hin1II CATG 5 cut(s) 487, 682, 752, 805, 827
Hin6I GCGC 4 cut(s) 474, 493, 495, 693
HinP1I GCGC 4 cut(s) 474, 493, 495, 693
HincII GTYRAC 1 cut(s) 125
HindII GTYRAC 1 cut(s) 125
HindIII AAGCTT 1 cut(s) 706
HinfI GANTC 5 cut(s) 38, 75, 180, 593, 785
HpaII CCGG 1 cut(s) 145
HphI GGTGA 5 cut(s) 7, 157, 251, 332, 845
Hpy166II GTNNAC 5 cut(s) 104, 125, 211, 323, 772
Hpy188I TCNGA 4 cut(s) 197, 441, 553, 603
Hpy188III TCNNGA 7 cut(s) 133, 269, 398, 669, 701, 782, 797
Hpy8I GTNNAC 5 cut(s) 104, 125, 211, 323, 772
Hpy99I CGWCG 3 cut(s) 372, 399, 933
HpyAV CCTTC 4 cut(s) 22, 340, 476, 897
HpyCH4III ACNGT 3 cut(s) 187, 294, 776
HpyCH4IV ACGT 2 cut(s) 219, 370
HpyCH4V TGCA 4 cut(s) 29, 365, 376, 876
HpyF10VI GCNNNNNNNGC 2 cut(s) 241, 692
HpyF3I CTNAG 3 cut(s) 96, 422, 638
HpySE526I ACGT 2 cut(s) 219, 370
Hsp92II CATG 5 cut(s) 487, 682, 752, 805, 827
HspAI GCGC 4 cut(s) 474, 493, 495, 693
KflI GGGWCCC 1 cut(s) 455
KpnI GGTACC 2 cut(s) 568, 849
Ksp22I TGATCA 1 cut(s) 534
Kzo9I GATC 4 cut(s) 58, 160, 534, 675
LmnI GCTCC 6 cut(s) 286, 467, 490, 657, 760, 825
Lsp1109I GCAGC 4 cut(s) 99, 509, 569, 804
LweI GCATC 2 cut(s) 16, 724
MabI ACCWGGT 1 cut(s) 308
MaeII ACGT 2 cut(s) 219, 370
MaeIII GTNAC 5 cut(s) 181, 187, 227, 398, 833
MalI GATC 4 cut(s) 60, 162, 536, 677
MboI GATC 4 cut(s) 58, 160, 534, 675
MboII GAAGA 2 cut(s) 248, 257
MhlI GDGCHC 2 cut(s) 73, 757
MluCI AATT 4 cut(s) 198, 610, 652, 871
MlyI GAGTC 4 cut(s) 32, 69, 189, 602
MmeI TCCRAC 2 cut(s) 468, 708
MseI TTAA 1 cut(s) 414
MslI CAYNNNNRTG 2 cut(s) 482, 542
MspA1I CMGCKG 1 cut(s) 317
MspI CCGG 1 cut(s) 145
MspR9I CCNGG 4 cut(s) 145, 310, 644, 882
MvaI CCWGG 3 cut(s) 310, 644, 882
MvnI CGCG 4 cut(s) 495, 591, 693, 812
MwoI GCNNNNNNNGC 2 cut(s) 241, 692
NciI CCSGG 1 cut(s) 145
NdeII GATC 4 cut(s) 58, 160, 534, 675
NlaIII CATG 5 cut(s) 487, 682, 752, 805, 827
NlaIV GGNNCC 5 cut(s) 88, 456, 457, 566, 847
NmuCI GTSAC 4 cut(s) 181, 187, 398, 833
NspI RCATGY 1 cut(s) 487
PauI GCGCGC 1 cut(s) 493
PciI ACATGT 1 cut(s) 483
PcsI WCGNNNNNNNCGW 1 cut(s) 697
PfeI GAWTC 1 cut(s) 785
PflFI GACNNNGTC 1 cut(s) 179
PkrI GCNGC 4 cut(s) 114, 499, 559, 819
PleI GAGTC 4 cut(s) 32, 69, 188, 601
PpsI GAGTC 4 cut(s) 32, 69, 188, 601
Ppu21I YACGTR 1 cut(s) 220
PpuMI RGGWCCY 1 cut(s) 455
PscI ACATGT 1 cut(s) 483
Psp124BI GAGCTC 1 cut(s) 73
Psp5II RGGWCCY 1 cut(s) 455
Psp6I CCWGG 3 cut(s) 308, 642, 880
PspFI CCCAGC 2 cut(s) 277, 458
PspGI CCWGG 3 cut(s) 308, 642, 880
PspN4I GGNNCC 5 cut(s) 88, 456, 457, 566, 847
PspPI GGNCC 2 cut(s) 455, 884
PspPPI RGGWCCY 1 cut(s) 455
PsrI GAACNNNNNNTAC 2 cut(s) 762, 794
PsyI GACNNNGTC 1 cut(s) 179
PteI GCGCGC 1 cut(s) 493
PvuII CAGCTG 1 cut(s) 317
RsaI GTAC 3 cut(s) 566, 715, 847
RsaNI GTAC 3 cut(s) 565, 714, 846
RseI CAYNNNNRTG 2 cut(s) 482, 542
SacI GAGCTC 1 cut(s) 73
SalI GTCGAC 1 cut(s) 123
SaqAI TTAA 1 cut(s) 414
SatI GCNGC 4 cut(s) 113, 498, 558, 818
Sau3AI GATC 4 cut(s) 58, 160, 534, 675
Sau96I GGNCC 2 cut(s) 455, 884
ScaI AGTACT 1 cut(s) 715
SchI GAGTC 4 cut(s) 32, 69, 189, 602
ScrFI CCNGG 4 cut(s) 145, 310, 644, 882
SduI GDGCHC 2 cut(s) 73, 757
SexAI ACCWGGT 1 cut(s) 308
SfaNI GCATC 2 cut(s) 16, 724
SinI GGWCC 2 cut(s) 455, 884
SmiMI CAYNNNNRTG 2 cut(s) 482, 542
SmlI CTYRAG 1 cut(s) 46
SmoI CTYRAG 1 cut(s) 46
Sse9I AATT 4 cut(s) 198, 610, 652, 871
SsiI CCGC 2 cut(s) 518, 589
SstI GAGCTC 1 cut(s) 73
StyD4I CCNGG 4 cut(s) 143, 308, 642, 880
StyI CCWWGG 1 cut(s) 853
TaaI ACNGT 3 cut(s) 187, 294, 776
TaiI ACGT 2 cut(s) 222, 373
TaqI TCGA 5 cut(s) 73, 124, 288, 700, 781
TaqII GACCGA 3 cut(s) 437, 843, 855
TasI AATT 4 cut(s) 198, 610, 652, 871
TatI WGTACW 1 cut(s) 713
TfiI GAWTC 1 cut(s) 785
Tru1I TTAA 1 cut(s) 414
Tru9I TTAA 1 cut(s) 414
TscAI CASTG 2 cut(s) 190, 781
TseFI GTSAC 4 cut(s) 181, 187, 398, 833
TseI GCWGC 4 cut(s) 112, 497, 557, 817
Tsp45I GTSAC 4 cut(s) 181, 187, 398, 833
TspDTI ATGAA 6 cut(s) 43, 396, 458, 603, 777, 781
TspGWI ACGGA 1 cut(s) 192
TspRI CASTG 2 cut(s) 190, 781
Tth111I GACNNNGTC 1 cut(s) 179
VpaK11BI GGWCC 2 cut(s) 455, 884
XapI RAATTY 1 cut(s) 610
XceI RCATGY 1 cut(s) 487
XcmI CCANNNNNNNNNTGG 1 cut(s) 484
XmiI GTMKAC 2 cut(s) 103, 124
ZrmI AGTACT 1 cut(s) 715
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.