Prupe.1G294700_v2.0.a1

rRNA N-glycosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0018098
Physical Location & Seq
Forward (+)
29186595 .. 29188672
2078 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G294700.1

Sequence Viewer

Length: 885 bp
ATGGAACTATCCTTCTCCACTAAGAACACAACCCCCCAGAAATATCGCGACTTCATAGAATCTCTGCGACAACGACTCACAGCCGGACGATCTAAATCACATGGTATACCGGTGCTGCCTAGAAGAGAAGATGTGCCAGACGCACAACGCTTTCTTCTTGTTGACCTCACAAACTCTGGAAACAATACCATCAGGCTCGCAATCGATGTGGTGAACGCTTACGTGGTGGGTTATGCTGCAGGTGGCCGATCCTACTTTCTTAAAGAAAATGCTCGTGAGAATCCGCCTCCAATTCACACCTTGTTCCGCGACACCACTCGGATGCCTCCTCTGGACTTCGACGGCAGCTACACAGGGCTTTCGCGAGCTGCGCAAGAAGCAGTGAAACGGAATATAGCACGTGATCGTGCTCGTAACCCAGCAGTGGCAGGTCTGCATCCGGATACACCTATTCTTGAGCGGATACCTCTGGGACGCAATGAACTAGATGACGCCATCAACCTTTTGCGCTTGGCGCCATCCCAATCCGACCAAGCTGTAGGGTTTATAGTGGTAATTCAGATGATCTGCGAAGCGGCAAGGTTTCGGTTCATTGAGGGTCTACTCCGCAATAGTATGAAGGATGTCTACGATCCCACTATACCAGGCCCTGCAACTCGAAGCCTGGAAAACCATTGGTCTGATATCTCTAAAGAGATCCAGAGGGTTCCGGCCAATCAAACACAATTCCAGAAGGCTGTTGTGCTCCATAATATCAAAAATGAACGCGTTGAAGTACGCAGTGTTGACTCAGACGTTGTTCGGGGTGTCGCTATGCTGCTCTACGACCGAAACCAAAATGCCAATCCTGGACCTTCAGCGAAAAAGCCTCTGTTAAAGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RIP

Protein Analysis

295

Amino Acids

32.96

Weight (kDa)

9.67

Isoelectric Point (pI)

39.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 230
Acc16I TGCGCA 1 cut(s) 372
Acc36I ACCTGC 2 cut(s) 230, 419
AccB1I GGYRCC 1 cut(s) 514
AccBSI CCGCTC 1 cut(s) 460
AccI GTMKAC 3 cut(s) 106, 601, 627
AccII CGCG 4 cut(s) 48, 309, 364, 768
AccIII TCCGGA 1 cut(s) 439
AciI CCGC 5 cut(s) 284, 307, 460, 575, 607
AclWI GGATC 3 cut(s) 243, 626, 691
AcoI YGGCCR 2 cut(s) 244, 711
AcuI CTGAAG 1 cut(s) 840
AcvI CACGTG 1 cut(s) 401
AcyI GRCGYC 2 cut(s) 492, 515
AfaI GTAC 1 cut(s) 777
AfiI CCNNNNNNNGG 1 cut(s) 424
AflIII ACRYGT 1 cut(s) 766
AgeI ACCGGT 1 cut(s) 109
AgsI TTSAA 1 cut(s) 773
AjnI CCWGG 3 cut(s) 643, 663, 847
AluBI AGCT 3 cut(s) 348, 368, 536
AluI AGCT 3 cut(s) 348, 368, 536
Alw21I GWGCWC 2 cut(s) 412, 747
AlwI GGATC 3 cut(s) 243, 626, 691
AlwNI CAGNNNCTG 1 cut(s) 650
Aor13HI TCCGGA 1 cut(s) 439
AoxI GGCC 3 cut(s) 244, 646, 711
ApeKI GCWGC 5 cut(s) 115, 236, 345, 368, 817
AsiGI ACCGGT 1 cut(s) 109
AspLEI GCGC 3 cut(s) 373, 510, 517
AspS9I GGNCC 2 cut(s) 647, 851
AsuHPI GGTGA 1 cut(s) 223
AvaII GGWCC 1 cut(s) 851
BanI GGYRCC 1 cut(s) 514
BarI GAAGNNNNNNTAC 2 cut(s) 611, 643
BauI CACGAG 1 cut(s) 273
BbrPI CACGTG 1 cut(s) 401
Bbv12I GWGCWC 2 cut(s) 412, 747
BbvI GCAGC 5 cut(s) 102, 223, 355, 357, 804
BccI CCATC 3 cut(s) 197, 503, 526
BceAI ACGGC 1 cut(s) 358
BciT130I CCWGG 3 cut(s) 645, 665, 849
BciVI GTATCC 2 cut(s) 436, 456
BfaI CTAG 2 cut(s) 120, 485
BfmI CTRYAG 2 cut(s) 237, 537
BfoI RGCGCY 1 cut(s) 518
BfuAI ACCTGC 2 cut(s) 230, 419
BfuI GTATCC 2 cut(s) 436, 456
BisI GCNGC 6 cut(s) 116, 237, 346, 369, 576, 818
BlsI GCNGC 6 cut(s) 117, 238, 347, 370, 577, 819
Bme1390I CCNGG 3 cut(s) 645, 665, 849
Bme18I GGWCC 1 cut(s) 851
BmgT120I GGNCC 2 cut(s) 647, 851
BmiI GGNNCC 2 cut(s) 516, 708
BmrFI CCNGG 3 cut(s) 645, 665, 849
BmsI GCATC 2 cut(s) 312, 445
BpuEI CTTGAG 1 cut(s) 476
Bsa29I ATCGAT 1 cut(s) 204
BsaAI YACGTR 2 cut(s) 223, 401
BsaBI GATNNNNATC 1 cut(s) 94
BsaHI GRCGYC 2 cut(s) 492, 515
BsaWI WCCGGW 2 cut(s) 109, 439
Bsc4I CCNNNNNNNGG 1 cut(s) 424
Bse118I RCCGGY 1 cut(s) 109
Bse3DI GCAATG 1 cut(s) 484
Bse8I GATNNNNATC 1 cut(s) 94
BseAI TCCGGA 1 cut(s) 439
BseBI CCWGG 3 cut(s) 645, 665, 849
BseCI ATCGAT 1 cut(s) 204
BseGI GGATG 4 cut(s) 327, 436, 518, 628
BseJI GATNNNNATC 1 cut(s) 94
BseLI CCNNNNNNNGG 1 cut(s) 424
BseMI GCAATG 1 cut(s) 484
BseMII CTCAG 1 cut(s) 804
BseRI GAGGAG 1 cut(s) 318
BseXI GCAGC 5 cut(s) 102, 223, 355, 357, 804
BseYI CCCAGC 1 cut(s) 418
Bsh1236I CGCG 4 cut(s) 48, 309, 364, 768
Bsh1285I CGRYCG 1 cut(s) 829
BshFI GGCC 3 cut(s) 246, 648, 713
BshNI GGYRCC 1 cut(s) 514
BshTI ACCGGT 1 cut(s) 109
BshVI ATCGAT 1 cut(s) 204
BsiEI CGRYCG 1 cut(s) 829
BsiHKAI GWGCWC 2 cut(s) 412, 747
BsiSI CCGG 4 cut(s) 84, 110, 440, 710
BslFI GGGAC 1 cut(s) 486
BslI CCNNNNNNNGG 1 cut(s) 424
BsmFI GGGAC 1 cut(s) 486
BsnI GGCC 3 cut(s) 246, 648, 713
Bsp1286I GDGCHC 2 cut(s) 412, 747
Bsp13I TCCGGA 1 cut(s) 439
Bsp143I GATC 6 cut(s) 89, 248, 403, 564, 631, 696
Bsp68I TCGCGA 2 cut(s) 48, 364
BspACI CCGC 5 cut(s) 284, 307, 460, 575, 607
BspANI GGCC 3 cut(s) 246, 648, 713
BspCNI CTCAG 1 cut(s) 803
BspDI ATCGAT 1 cut(s) 204
BspEI TCCGGA 1 cut(s) 439
BspFNI CGCG 4 cut(s) 48, 309, 364, 768
BspLI GGNNCC 2 cut(s) 516, 708
BspMAI CTGCAG 1 cut(s) 241
BspMI ACCTGC 2 cut(s) 230, 419
BspPI GGATC 3 cut(s) 243, 626, 691
BspT107I GGYRCC 1 cut(s) 514
BsrBI CCGCTC 1 cut(s) 460
BsrDI GCAATG 1 cut(s) 484
BsrFI RCCGGY 1 cut(s) 109
BssAI RCCGGY 1 cut(s) 109
BssMI GATC 6 cut(s) 89, 248, 403, 564, 631, 696
BssNAI GTATAC 1 cut(s) 107
BssNI GRCGYC 2 cut(s) 492, 515
BssSI CACGAG 1 cut(s) 273
Bst1107I GTATAC 1 cut(s) 107
Bst2BI CACGAG 1 cut(s) 273
Bst2UI CCWGG 3 cut(s) 645, 665, 849
Bst6I CTCTTC 1 cut(s) 118
BstACI GRCGYC 2 cut(s) 492, 515
BstBAI YACGTR 2 cut(s) 223, 401
BstC8I GCNNGC 2 cut(s) 198, 366
BstDEI CTNAG 2 cut(s) 21, 790
BstF5I GGATG 4 cut(s) 327, 436, 518, 628
BstFNI CGCG 4 cut(s) 48, 309, 364, 768
BstH2I RGCGCY 1 cut(s) 518
BstHHI GCGC 3 cut(s) 373, 510, 517
BstKTI GATC 6 cut(s) 92, 251, 406, 567, 634, 699
BstMBI GATC 6 cut(s) 89, 248, 403, 564, 631, 696
BstMCI CGRYCG 1 cut(s) 829
BstMWI GCNNNNNNNGC 3 cut(s) 370, 377, 514
BstNI CCWGG 3 cut(s) 645, 665, 849
BstSCI CCNGG 3 cut(s) 643, 663, 847
BstSFI CTRYAG 2 cut(s) 237, 537
BstUI CGCG 4 cut(s) 48, 309, 364, 768
BstV1I GCAGC 5 cut(s) 102, 223, 355, 357, 804
BstX2I RGATCY 1 cut(s) 696
BstYI RGATCY 1 cut(s) 696
BstZ17I GTATAC 1 cut(s) 107
Bsu15I ATCGAT 1 cut(s) 204
BsuI GTATCC 2 cut(s) 436, 456
BsuRI GGCC 3 cut(s) 246, 648, 713
BsuTUI ATCGAT 1 cut(s) 204
BtsCI GGATG 4 cut(s) 327, 436, 518, 628
BtsI GCAGTG 3 cut(s) 387, 429, 787
BtsIMutI CAGTG 3 cut(s) 387, 429, 787
BtuMI TCGCGA 2 cut(s) 48, 364
BveI ACCTGC 2 cut(s) 230, 419
Cac8I GCNNGC 2 cut(s) 198, 366
CaiI CAGNNNCTG 1 cut(s) 650
CfoI GCGC 3 cut(s) 373, 510, 517
Cfr10I RCCGGY 1 cut(s) 109
Cfr13I GGNCC 2 cut(s) 647, 851
ClaI ATCGAT 1 cut(s) 204
CseI GACGC 3 cut(s) 149, 483, 500
Csp6I GTAC 1 cut(s) 776
CspAI ACCGGT 1 cut(s) 109
CspCI CAANNNNNGTGG 2 cut(s) 189, 224
CviAII CATG 1 cut(s) 101
CviQI GTAC 1 cut(s) 776
DdeI CTNAG 2 cut(s) 21, 790
DinI GGCGCC 1 cut(s) 516
DpnI GATC 6 cut(s) 91, 250, 405, 566, 633, 698
DpnII GATC 6 cut(s) 89, 248, 403, 564, 631, 696
EaeI YGGCCR 2 cut(s) 244, 711
Eam1104I CTCTTC 1 cut(s) 118
EarI CTCTTC 1 cut(s) 118
EciI GGCGGA 1 cut(s) 273
Eco32I GATATC 1 cut(s) 685
Eco47I GGWCC 1 cut(s) 851
Eco57I CTGAAG 1 cut(s) 840
Eco72I CACGTG 1 cut(s) 401
EcoO109I RGGNCCY 1 cut(s) 647
EcoRII CCWGG 3 cut(s) 643, 663, 847
EcoRV GATATC 1 cut(s) 685
EgeI GGCGCC 1 cut(s) 516
EheI GGCGCC 1 cut(s) 516
FaeI CATG 1 cut(s) 104
FaqI GGGAC 1 cut(s) 486
FatI CATG 1 cut(s) 100
FblI GTMKAC 3 cut(s) 106, 601, 627
Fnu4HI GCNGC 6 cut(s) 116, 237, 346, 369, 576, 818
FokI GGATG 4 cut(s) 334, 423, 505, 635
Fsp4HI GCNGC 6 cut(s) 116, 237, 346, 369, 576, 818
FspBI CTAG 2 cut(s) 120, 485
FspI TGCGCA 1 cut(s) 372
GlaI GCGC 3 cut(s) 372, 509, 516
GluI GCNGC 6 cut(s) 116, 237, 346, 369, 576, 818
GsaI CCCAGC 1 cut(s) 422
HaeII RGCGCY 1 cut(s) 518
HaeIII GGCC 3 cut(s) 246, 648, 713
HapII CCGG 4 cut(s) 84, 110, 440, 710
HgaI GACGC 3 cut(s) 149, 483, 500
HhaI GCGC 3 cut(s) 373, 510, 517
Hin1I GRCGYC 2 cut(s) 492, 515
Hin1II CATG 1 cut(s) 104
Hin6I GCGC 3 cut(s) 371, 508, 515
HinP1I GCGC 3 cut(s) 371, 508, 515
HincII GTYRAC 2 cut(s) 163, 787
HindII GTYRAC 2 cut(s) 163, 787
HinfI GANTC 4 cut(s) 59, 75, 280, 788
HpaII CCGG 4 cut(s) 84, 110, 440, 710
HphI GGTGA 1 cut(s) 223
Hpy166II GTNNAC 6 cut(s) 107, 163, 214, 602, 628, 787
Hpy188I TCNGA 5 cut(s) 321, 529, 561, 682, 793
Hpy188III TCNNGA 9 cut(s) 47, 177, 275, 332, 363, 440, 455, 700, 730
Hpy8I GTNNAC 6 cut(s) 107, 163, 214, 602, 628, 787
Hpy99I CGWCG 1 cut(s) 344
HpyAV CCTTC 4 cut(s) 22, 613, 727, 864
HpyCH4IV ACGT 3 cut(s) 222, 400, 795
HpyCH4V TGCA 3 cut(s) 239, 436, 653
HpyF10VI GCNNNNNNNGC 3 cut(s) 370, 377, 514
HpyF3I CTNAG 2 cut(s) 21, 790
HpySE526I ACGT 3 cut(s) 222, 400, 795
Hsp92I GRCGYC 2 cut(s) 492, 515
Hsp92II CATG 1 cut(s) 104
HspAI GCGC 3 cut(s) 371, 508, 515
KasI GGCGCC 1 cut(s) 514
Kpn2I TCCGGA 1 cut(s) 439
Kzo9I GATC 6 cut(s) 89, 248, 403, 564, 631, 696
LmnI GCTCC 1 cut(s) 750
Lsp1109I GCAGC 5 cut(s) 102, 223, 355, 357, 804
LweI GCATC 2 cut(s) 312, 445
MaeI CTAG 2 cut(s) 120, 485
MaeII ACGT 3 cut(s) 222, 400, 795
MaeIII GTNAC 1 cut(s) 413
MalI GATC 6 cut(s) 91, 250, 405, 566, 633, 698
MbiI CCGCTC 1 cut(s) 460
MboI GATC 6 cut(s) 89, 248, 403, 564, 631, 696
MboII GAAGA 3 cut(s) 135, 140, 146
MflI RGATCY 1 cut(s) 696
MhlI GDGCHC 2 cut(s) 412, 747
MluCI AATT 4 cut(s) 291, 555, 725, 880
MluI ACGCGT 1 cut(s) 766
Mly113I GGCGCC 1 cut(s) 515
MlyI GAGTC 2 cut(s) 69, 782
MmeI TCCRAC 1 cut(s) 552
MnlI CCTC 8 cut(s) 176, 297, 336, 339, 477, 589, 696, 879
MroI TCCGGA 1 cut(s) 439
MseI TTAA 3 cut(s) 261, 875, 883
MslI CAYNNNNRTG 1 cut(s) 320
MspI CCGG 4 cut(s) 84, 110, 440, 710
MspR9I CCNGG 3 cut(s) 645, 665, 849
MvaI CCWGG 3 cut(s) 645, 665, 849
MvnI CGCG 4 cut(s) 48, 309, 364, 768
MwoI GCNNNNNNNGC 3 cut(s) 370, 377, 514
NarI GGCGCC 1 cut(s) 515
NdeII GATC 6 cut(s) 89, 248, 403, 564, 631, 696
NlaIII CATG 1 cut(s) 104
NlaIV GGNNCC 2 cut(s) 516, 708
NruI TCGCGA 2 cut(s) 48, 364
NsbI TGCGCA 1 cut(s) 372
PaqCI CACCTGC 1 cut(s) 230
PfeI GAWTC 2 cut(s) 59, 280
PfoI TCCNGGA 1 cut(s) 847
PinAI ACCGGT 1 cut(s) 109
PkrI GCNGC 6 cut(s) 117, 238, 347, 370, 577, 819
PleI GAGTC 2 cut(s) 69, 782
PluTI GGCGCC 1 cut(s) 518
PmaCI CACGTG 1 cut(s) 401
PmlI CACGTG 1 cut(s) 401
PpsI GAGTC 2 cut(s) 69, 782
Ppu21I YACGTR 2 cut(s) 223, 401
Psp6I CCWGG 3 cut(s) 643, 663, 847
PspCI CACGTG 1 cut(s) 401
PspFI CCCAGC 1 cut(s) 418
PspGI CCWGG 3 cut(s) 643, 663, 847
PspN4I GGNNCC 2 cut(s) 516, 708
PspPI GGNCC 2 cut(s) 647, 851
PstI CTGCAG 1 cut(s) 241
PstNI CAGNNNCTG 1 cut(s) 650
PsuI RGATCY 1 cut(s) 696
RruI TCGCGA 2 cut(s) 48, 364
RsaI GTAC 1 cut(s) 777
RsaNI GTAC 1 cut(s) 776
RseI CAYNNNNRTG 1 cut(s) 320
SaqAI TTAA 3 cut(s) 261, 875, 883
SatI GCNGC 6 cut(s) 116, 237, 346, 369, 576, 818
Sau3AI GATC 6 cut(s) 89, 248, 403, 564, 631, 696
Sau96I GGNCC 2 cut(s) 647, 851
SchI GAGTC 2 cut(s) 69, 782
ScrFI CCNGG 3 cut(s) 645, 665, 849
SduI GDGCHC 2 cut(s) 412, 747
SfaNI GCATC 2 cut(s) 312, 445
SfcI CTRYAG 2 cut(s) 237, 537
SfoI GGCGCC 1 cut(s) 516
SinI GGWCC 1 cut(s) 851
SmiMI CAYNNNNRTG 1 cut(s) 320
SmlI CTYRAG 1 cut(s) 455
SmoI CTYRAG 1 cut(s) 455
Sse9I AATT 4 cut(s) 291, 555, 725, 880
SsiI CCGC 5 cut(s) 284, 307, 460, 575, 607
SspDI GGCGCC 1 cut(s) 514
SspMI CTAG 2 cut(s) 120, 485
StyD4I CCNGG 3 cut(s) 643, 663, 847
TaiI ACGT 3 cut(s) 225, 403, 798
TaqI TCGA 3 cut(s) 204, 339, 658
TaqII GACCGA 1 cut(s) 843
TasI AATT 4 cut(s) 291, 555, 725, 880
TauI GCSGC 1 cut(s) 578
TfiI GAWTC 2 cut(s) 59, 280
Tru1I TTAA 3 cut(s) 261, 875, 883
Tru9I TTAA 3 cut(s) 261, 875, 883
TscAI CASTG 3 cut(s) 387, 429, 787
TseI GCWGC 5 cut(s) 115, 236, 345, 368, 817
TspDTI ATGAA 5 cut(s) 43, 495, 580, 632, 777
TspGWI ACGGA 1 cut(s) 403
TspRI CASTG 3 cut(s) 387, 429, 787
VpaK11BI GGWCC 1 cut(s) 851
XmiI GTMKAC 3 cut(s) 106, 601, 627
XspI CTAG 2 cut(s) 120, 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.