pycom14g10770

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0018098
Physical Location & Seq
Forward (+)
13537974 .. 13538333
360 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g10770.1

Sequence Viewer

Length: 360 bp
ATGTGGTGGCTTATGTACAAATCCTACTTTCTCAATGACGCTCCGGTTGCAGCTCTCAACACTTTATTCCCTCCCACAACGAGCTTTTATCTTCCATTTACTTCTAGCTATACTGACCTCGAGAAAGCTGGTCGTAAATCCAGGTGGGAAATCCCTCTAGGACTCACTCCCCTGGACAATGCCATCACCGCGTTGTGGGAGGGAGGTGATTCCAAAGCCGCGCGGTCCCTTCTGGTGGCCACTCAGATGGTTTTAGAAGCAGCGAAGTCCATATTAGTTGAGCAACGTGTCCGGGATAGCATACAAGACAAAGGTGACTTCGTACCGGACGCTGGAATCCTAGCCAACAGGGAATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RIP Ricin_B_lectin

Protein Analysis

120

Amino Acids

13.33

Weight (kDa)

5.04

Isoelectric Point (pI)

47.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RIP PF00161 21 - 105 4.2e-15 Ribosome inactivating protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 191, 221, 223
AciI CCGC 3 cut(s) 189, 219, 223
AcoI YGGCCR 1 cut(s) 237
AcsI RAATTY 1 cut(s) 353
AfaI GTAC 2 cut(s) 17, 324
AfiI CCNNNNNNNGG 3 cut(s) 195, 235, 332
AflIII ACRYGT 1 cut(s) 286
AjnI CCWGG 2 cut(s) 140, 171
AjuI GAANNNNNNNTTGG 1 cut(s) 338
AluBI AGCT 4 cut(s) 53, 84, 108, 128
AluI AGCT 4 cut(s) 53, 84, 108, 128
Ama87I CYCGRG 1 cut(s) 119
AoxI GGCC 1 cut(s) 237
ApeKI GCWGC 2 cut(s) 50, 260
ApoI RAATTY 1 cut(s) 353
AspLEI GCGC 1 cut(s) 223
AspS9I GGNCC 1 cut(s) 225
AsuC2I CCSGG 1 cut(s) 293
AsuHPI GGTGA 3 cut(s) 178, 218, 326
AvaI CYCGRG 1 cut(s) 119
AvaII GGWCC 1 cut(s) 225
BalI TGGCCA 1 cut(s) 239
BbvI GCAGC 2 cut(s) 62, 272
BccI CCATC 2 cut(s) 191, 241
BciT130I CCWGG 2 cut(s) 142, 173
BcnI CCSGG 1 cut(s) 293
BfaI CTAG 4 cut(s) 105, 158, 341, 358
BisI GCNGC 3 cut(s) 51, 219, 261
BlsI GCNGC 3 cut(s) 52, 220, 262
Bme1390I CCNGG 3 cut(s) 142, 173, 293
Bme18I GGWCC 1 cut(s) 225
BmeT110I CYCGRG 1 cut(s) 119
BmgT120I GGNCC 1 cut(s) 225
BmiI GGNNCC 1 cut(s) 227
BmrFI CCNGG 3 cut(s) 142, 173, 293
BpuMI CCSGG 1 cut(s) 293
BsaJI CCNNGG 1 cut(s) 171
BsaWI WCCGGW 2 cut(s) 43, 325
Bsc4I CCNNNNNNNGG 3 cut(s) 195, 235, 332
BseBI CCWGG 2 cut(s) 142, 173
BseDI CCNNGG 1 cut(s) 171
BseLI CCNNNNNNNGG 3 cut(s) 195, 235, 332
BseMII CTCAG 1 cut(s) 257
BseXI GCAGC 2 cut(s) 62, 272
Bsh1236I CGCG 3 cut(s) 191, 221, 223
BshFI GGCC 1 cut(s) 239
BsiHKCI CYCGRG 1 cut(s) 119
BsiSI CCGG 3 cut(s) 44, 292, 326
BslFI GGGAC 1 cut(s) 211
BslI CCNNNNNNNGG 3 cut(s) 195, 235, 332
BsmFI GGGAC 1 cut(s) 211
BsnI GGCC 1 cut(s) 239
BsoBI CYCGRG 1 cut(s) 119
Bsp1407I TGTACA 1 cut(s) 15
BspACI CCGC 3 cut(s) 189, 219, 223
BspANI GGCC 1 cut(s) 239
BspCNI CTCAG 1 cut(s) 256
BspFNI CGCG 3 cut(s) 191, 221, 223
BspLI GGNNCC 1 cut(s) 227
BsrGI TGTACA 1 cut(s) 15
BssECI CCNNGG 1 cut(s) 171
Bst2UI CCWGG 2 cut(s) 142, 173
BstAUI TGTACA 1 cut(s) 15
BstDEI CTNAG 1 cut(s) 243
BstFNI CGCG 3 cut(s) 191, 221, 223
BstHHI GCGC 1 cut(s) 223
BstMWI GCNNNNNNNGC 2 cut(s) 47, 188
BstNI CCWGG 2 cut(s) 142, 173
BstSCI CCNGG 3 cut(s) 140, 171, 291
BstUI CGCG 3 cut(s) 191, 221, 223
BstV1I GCAGC 2 cut(s) 62, 272
BstXI CCANNNNNNTGG 1 cut(s) 247
BsuRI GGCC 1 cut(s) 239
CfoI GCGC 1 cut(s) 223
Cfr13I GGNCC 1 cut(s) 225
CseI GACGC 2 cut(s) 47, 338
Csp6I GTAC 2 cut(s) 16, 323
CviJI RGCY 8 cut(s) 10, 53, 84, 108, 128, 218, 239, 344
CviKI_1 RGCY 8 cut(s) 10, 53, 84, 108, 128, 218, 239, 344
CviQI GTAC 2 cut(s) 16, 323
DdeI CTNAG 1 cut(s) 243
EaeI YGGCCR 1 cut(s) 237
Eco47I GGWCC 1 cut(s) 225
Eco88I CYCGRG 1 cut(s) 119
EcoRI GAATTC 1 cut(s) 353
EcoRII CCWGG 2 cut(s) 140, 171
FaiI YATR 4 cut(s) 14, 111, 272, 302
FaqI GGGAC 1 cut(s) 211
Fnu4HI GCNGC 3 cut(s) 51, 219, 261
Fsp4HI GCNGC 3 cut(s) 51, 219, 261
FspBI CTAG 4 cut(s) 105, 158, 341, 358
GlaI GCGC 1 cut(s) 222
GluI GCNGC 3 cut(s) 51, 219, 261
HaeIII GGCC 1 cut(s) 239
HapII CCGG 3 cut(s) 44, 292, 326
HgaI GACGC 2 cut(s) 47, 338
HhaI GCGC 1 cut(s) 223
Hin6I GCGC 1 cut(s) 221
HinP1I GCGC 1 cut(s) 221
HinfI GANTC 3 cut(s) 162, 209, 336
HpaII CCGG 3 cut(s) 44, 292, 326
HphI GGTGA 3 cut(s) 178, 218, 326
Hpy188I TCNGA 1 cut(s) 246
Hpy188III TCNNGA 1 cut(s) 121
HpyAV CCTTC 1 cut(s) 239
HpyCH4IV ACGT 1 cut(s) 286
HpyCH4V TGCA 1 cut(s) 50
HpyF10VI GCNNNNNNNGC 2 cut(s) 47, 188
HpyF3I CTNAG 1 cut(s) 243
HpySE526I ACGT 1 cut(s) 286
HspAI GCGC 1 cut(s) 221
LmnI GCTCC 1 cut(s) 46
Lsp1109I GCAGC 2 cut(s) 62, 272
MaeI CTAG 4 cut(s) 105, 158, 341, 358
MaeII ACGT 1 cut(s) 286
MaeIII GTNAC 1 cut(s) 314
MboII GAAGA 1 cut(s) 83
MlsI TGGCCA 1 cut(s) 239
MluCI AATT 1 cut(s) 353
MluNI TGGCCA 1 cut(s) 239
MlyI GAGTC 1 cut(s) 156
MnlI CCTC 5 cut(s) 81, 128, 165, 193, 197
Mox20I TGGCCA 1 cut(s) 239
MscI TGGCCA 1 cut(s) 239
MslI CAYNNNNRTG 1 cut(s) 245
Msp20I TGGCCA 1 cut(s) 239
MspI CCGG 3 cut(s) 44, 292, 326
MspR9I CCNGG 3 cut(s) 142, 173, 293
MvaI CCWGG 2 cut(s) 142, 173
MvnI CGCG 3 cut(s) 191, 221, 223
MwoI GCNNNNNNNGC 2 cut(s) 47, 188
NciI CCSGG 1 cut(s) 293
NlaIV GGNNCC 1 cut(s) 227
NmuCI GTSAC 1 cut(s) 314
PaeR7I CTCGAG 1 cut(s) 119
PfeI GAWTC 2 cut(s) 209, 336
PfoI TCCNGGA 1 cut(s) 291
PkrI GCNGC 3 cut(s) 52, 220, 262
PleI GAGTC 1 cut(s) 156
PpsI GAGTC 1 cut(s) 156
Psp6I CCWGG 2 cut(s) 140, 171
PspGI CCWGG 2 cut(s) 140, 171
PspN4I GGNNCC 1 cut(s) 227
PspPI GGNCC 1 cut(s) 225
RsaI GTAC 2 cut(s) 17, 324
RsaNI GTAC 2 cut(s) 16, 323
RseI CAYNNNNRTG 1 cut(s) 245
SatI GCNGC 3 cut(s) 51, 219, 261
Sau96I GGNCC 1 cut(s) 225
SchI GAGTC 1 cut(s) 156
ScrFI CCNGG 3 cut(s) 142, 173, 293
SetI ASST 9 cut(s) 55, 86, 110, 120, 130, 146, 208, 289, 316
Sfr274I CTCGAG 1 cut(s) 119
SinI GGWCC 1 cut(s) 225
SlaI CTCGAG 1 cut(s) 119
SmiMI CAYNNNNRTG 1 cut(s) 245
SmlI CTYRAG 1 cut(s) 119
SmoI CTYRAG 1 cut(s) 119
Sse9I AATT 1 cut(s) 353
SsiI CCGC 3 cut(s) 189, 219, 223
SspMI CTAG 4 cut(s) 105, 158, 341, 358
StyD4I CCNGG 3 cut(s) 140, 171, 291
TaiI ACGT 1 cut(s) 289
TaqI TCGA 1 cut(s) 120
TasI AATT 1 cut(s) 353
TatI WGTACW 1 cut(s) 15
TauI GCSGC 1 cut(s) 221
TfiI GAWTC 2 cut(s) 209, 336
TseFI GTSAC 1 cut(s) 314
TseI GCWGC 2 cut(s) 50, 260
Tsp45I GTSAC 1 cut(s) 314
VpaK11BI GGWCC 1 cut(s) 225
XapI RAATTY 1 cut(s) 353
XhoI CTCGAG 1 cut(s) 119
XspI CTAG 4 cut(s) 105, 158, 341, 358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.