Prupe.1G294900_v2.0.a1

rRNA N-glycosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0018098
Physical Location & Seq
Forward (+)
29192393 .. 29193217
825 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G294900.1

Sequence Viewer

Length: 825 bp
ATGGCACTCGTCTTCTCCACTAGGAATGCAACCCCCCAGACATACCACACCTTCATAGACGCTCTGCGACTAAGACTCACAGCCGGACAGCCCAAGTCACATGGTATACCGGTGCTGCCACGAAAAGAAGATGTGCAGAATGTGCAGCGCTTTCTGCTTGTTGATCTAACAAACTCTGAAAACAATACCATCACGGTGGCAATAGATGTGGTGAACGCTTACGTGGTGGGTTATGCTGCAGGTGGTCGATCCTACTTTTTTGCAGAAAATGCTCCTAACGATCGGCCTCCAATCCAAGTCTTATTCCCCGGCACCACTTGGGTTCCTACTCTGCTCTTCAACGGCACCTACAACGGGCTTTCGCGCGGTGCAGAAGAAGCAGTACGACGTCGGCGTGCTGGTAACCGTGACCCAAATATAGATGAGAAGACACCTGTTCTTGAGCAAATCTTTTTGGGCCGCAATCAACTAGATGAGGCCATCGGGCTGTTGCGGGGGGCCGTATCCCAACCGGAACAAGCCTTAGGGTTTGTTGTGATCATTCAAATGCTTTCGGAAGCGGCAAGGTTCCGACAAATTGAGGGATTGGTCCGCACTACCATGAAGGAAGAGTACGATCCCCTTAAGCGAGGCCTTTCACTGGCGAGCCTTGAAACCAAATGGTCTGATCTCTCCGAAGAGATCCAGACGGTACCGGCCAATCAAACACGCTTCCATAGGGCTATTACGGTCCACAACATCTCAAATGCACGCGTCGAAATAAACAGTGTTGAATCACCCTTTGTTCAGGGTGTCGCGATGCTGCTCTATAGAAACCGAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RIP

Protein Analysis

275

Amino Acids

30.67

Weight (kDa)

9.21

Isoelectric Point (pI)

45.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 230
AatII GACGTC 1 cut(s) 391
Acc36I ACCTGC 1 cut(s) 230
Acc65I GGTACC 1 cut(s) 691
AccB1I GGYRCC 3 cut(s) 311, 344, 691
AccI GTMKAC 1 cut(s) 106
AccII CGCG 4 cut(s) 364, 366, 753, 797
AciI CCGC 5 cut(s) 366, 460, 493, 560, 592
AclWI GGATC 3 cut(s) 243, 611, 676
AcoI YGGCCR 1 cut(s) 696
AcyI GRCGYC 1 cut(s) 388
AfaI GTAC 3 cut(s) 384, 614, 693
AfeI AGCGCT 1 cut(s) 149
AfiI CCNNNNNNNGG 2 cut(s) 354, 640
AflII CTTAAG 1 cut(s) 623
AflIII ACRYGT 1 cut(s) 751
AgeI ACCGGT 1 cut(s) 109
AgsI TTSAA 4 cut(s) 340, 545, 653, 773
AlwI GGATC 3 cut(s) 243, 611, 676
Aor51HI AGCGCT 1 cut(s) 149
AoxI GGCC 6 cut(s) 284, 457, 477, 498, 631, 696
ApeKI GCWGC 4 cut(s) 115, 145, 236, 802
AsiGI ACCGGT 1 cut(s) 109
Asp718I GGTACC 1 cut(s) 691
AspLEI GCGC 2 cut(s) 150, 366
AspS9I GGNCC 4 cut(s) 457, 498, 589, 730
AsuC2I CCSGG 1 cut(s) 309
AsuHPI GGTGA 2 cut(s) 223, 768
AvaII GGWCC 2 cut(s) 589, 730
AxyI CCTNAGG 1 cut(s) 523
BanI GGYRCC 3 cut(s) 311, 344, 691
BarI GAAGNNNNNNTAC 4 cut(s) 366, 398, 596, 628
BbsI GAAGAC 2 cut(s) 4, 434
BbvI GCAGC 4 cut(s) 102, 157, 223, 789
BccI CCATC 2 cut(s) 197, 488
BceAI ACGGC 2 cut(s) 358, 485
BciVI GTATCC 1 cut(s) 514
BclI TGATCA 1 cut(s) 537
BcnI CCSGG 1 cut(s) 309
BfaI CTAG 2 cut(s) 21, 470
BfmI CTRYAG 2 cut(s) 237, 808
BfoI RGCGCY 1 cut(s) 151
BfrI CTTAAG 1 cut(s) 623
BfuAI ACCTGC 1 cut(s) 230
BfuI GTATCC 1 cut(s) 514
BisI GCNGC 6 cut(s) 116, 146, 237, 460, 561, 803
BlsI GCNGC 6 cut(s) 117, 147, 238, 461, 562, 804
Bme1390I CCNGG 1 cut(s) 309
Bme18I GGWCC 2 cut(s) 589, 730
BmgT120I GGNCC 4 cut(s) 457, 498, 589, 730
BmiI GGNNCC 6 cut(s) 313, 324, 346, 499, 569, 693
BmrFI CCNGG 1 cut(s) 309
BmsI GCATC 1 cut(s) 789
BpiI GAAGAC 2 cut(s) 4, 434
BpuEI CTTGAG 1 cut(s) 461
BpuMI CCSGG 1 cut(s) 309
BsaAI YACGTR 1 cut(s) 223
BsaHI GRCGYC 1 cut(s) 388
BsaJI CCNNGG 1 cut(s) 307
BsaWI WCCGGW 2 cut(s) 109, 511
Bsc4I CCNNNNNNNGG 2 cut(s) 354, 640
Bse118I RCCGGY 2 cut(s) 109, 694
Bse1I ACTGG 1 cut(s) 645
Bse21I CCTNAGG 1 cut(s) 523
BseDI CCNNGG 1 cut(s) 307
BseLI CCNNNNNNNGG 2 cut(s) 354, 640
BseNI ACTGG 1 cut(s) 645
BseXI GCAGC 4 cut(s) 102, 157, 223, 789
BsgI GTGCAG 3 cut(s) 155, 164, 390
Bsh1236I CGCG 4 cut(s) 364, 366, 753, 797
Bsh1285I CGRYCG 1 cut(s) 283
BshFI GGCC 6 cut(s) 286, 459, 479, 500, 633, 698
BshNI GGYRCC 3 cut(s) 311, 344, 691
BshTI ACCGGT 1 cut(s) 109
BsiEI CGRYCG 1 cut(s) 283
BsiSI CCGG 5 cut(s) 84, 110, 309, 512, 695
BslI CCNNNNNNNGG 2 cut(s) 354, 640
BsmI GAATGC 1 cut(s) 31
BsnI GGCC 6 cut(s) 286, 459, 479, 500, 633, 698
Bsp143I GATC 7 cut(s) 163, 248, 280, 537, 616, 667, 681
Bsp68I TCGCGA 1 cut(s) 797
BspACI CCGC 5 cut(s) 366, 460, 493, 560, 592
BspANI GGCC 6 cut(s) 286, 459, 479, 500, 633, 698
BspFNI CGCG 4 cut(s) 364, 366, 753, 797
BspLI GGNNCC 6 cut(s) 313, 324, 346, 499, 569, 693
BspMAI CTGCAG 1 cut(s) 241
BspMI ACCTGC 1 cut(s) 230
BspPI GGATC 3 cut(s) 243, 611, 676
BspQI GCTCTTC 1 cut(s) 341
BspT107I GGYRCC 3 cut(s) 311, 344, 691
BspTI CTTAAG 1 cut(s) 623
BsrFI RCCGGY 2 cut(s) 109, 694
BsrI ACTGG 1 cut(s) 645
BssAI RCCGGY 2 cut(s) 109, 694
BssECI CCNNGG 1 cut(s) 307
BssMI GATC 7 cut(s) 163, 248, 280, 537, 616, 667, 681
BssNAI GTATAC 1 cut(s) 107
BssNI GRCGYC 1 cut(s) 388
Bst1107I GTATAC 1 cut(s) 107
Bst4CI ACNGT 5 cut(s) 196, 407, 691, 730, 767
Bst6I CTCTTC 3 cut(s) 341, 603, 672
BstACI GRCGYC 1 cut(s) 388
BstAFI CTTAAG 1 cut(s) 623
BstAPI GCANNNNNTGC 2 cut(s) 142, 269
BstBAI YACGTR 1 cut(s) 223
BstC8I GCNNGC 3 cut(s) 396, 646, 751
BstDEI CTNAG 2 cut(s) 71, 523
BstEII GGTNACC 1 cut(s) 401
BstFNI CGCG 4 cut(s) 364, 366, 753, 797
BstH2I RGCGCY 1 cut(s) 151
BstHHI GCGC 2 cut(s) 150, 366
BstKTI GATC 7 cut(s) 166, 251, 283, 540, 619, 670, 684
BstMBI GATC 7 cut(s) 163, 248, 280, 537, 616, 667, 681
BstMCI CGRYCG 1 cut(s) 283
BstMWI GCNNNNNNNGC 4 cut(s) 142, 154, 269, 377
BstPI GGTNACC 1 cut(s) 401
BstSCI CCNGG 1 cut(s) 307
BstSFI CTRYAG 2 cut(s) 237, 808
BstUI CGCG 4 cut(s) 364, 366, 753, 797
BstV1I GCAGC 4 cut(s) 102, 157, 223, 789
BstV2I GAAGAC 2 cut(s) 4, 434
BstX2I RGATCY 1 cut(s) 681
BstXI CCANNNNNNTGG 1 cut(s) 196
BstYI RGATCY 1 cut(s) 681
BstZ17I GTATAC 1 cut(s) 107
Bsu36I CCTNAGG 1 cut(s) 523
BsuI GTATCC 1 cut(s) 514
BsuRI GGCC 6 cut(s) 286, 459, 479, 500, 633, 698
BtgZI GCGATG 1 cut(s) 812
BtsIMutI CAGTG 2 cut(s) 638, 772
BtuMI TCGCGA 1 cut(s) 797
BveI ACCTGC 1 cut(s) 230
Cac8I GCNNGC 3 cut(s) 396, 646, 751
CfoI GCGC 2 cut(s) 150, 366
Cfr10I RCCGGY 2 cut(s) 109, 694
Cfr13I GGNCC 4 cut(s) 457, 498, 589, 730
CseI GACGC 2 cut(s) 68, 742
Csp6I GTAC 3 cut(s) 383, 613, 692
CspAI ACCGGT 1 cut(s) 109
CspCI CAANNNNNGTGG 2 cut(s) 189, 224
CviAII CATG 2 cut(s) 101, 601
CviQI GTAC 3 cut(s) 383, 613, 692
DdeI CTNAG 2 cut(s) 71, 523
DpnI GATC 7 cut(s) 165, 250, 282, 539, 618, 669, 683
DpnII GATC 7 cut(s) 163, 248, 280, 537, 616, 667, 681
EaeI YGGCCR 1 cut(s) 696
Eam1104I CTCTTC 3 cut(s) 341, 603, 672
EarI CTCTTC 3 cut(s) 341, 603, 672
Eco147I AGGCCT 1 cut(s) 633
Eco47I GGWCC 2 cut(s) 589, 730
Eco47III AGCGCT 1 cut(s) 149
Eco81I CCTNAGG 1 cut(s) 523
Eco91I GGTNACC 1 cut(s) 401
EcoO65I GGTNACC 1 cut(s) 401
FaeI CATG 2 cut(s) 104, 604
FaiI YATR 9 cut(s) 43, 56, 102, 107, 234, 419, 602, 717, 810
FatI CATG 2 cut(s) 100, 600
FauI CCCGC 1 cut(s) 486
FbaI TGATCA 1 cut(s) 537
FblI GTMKAC 1 cut(s) 106
Fnu4HI GCNGC 6 cut(s) 116, 146, 237, 460, 561, 803
Fsp4HI GCNGC 6 cut(s) 116, 146, 237, 460, 561, 803
FspBI CTAG 2 cut(s) 21, 470
GlaI GCGC 2 cut(s) 149, 365
GluI GCNGC 6 cut(s) 116, 146, 237, 460, 561, 803
HaeII RGCGCY 1 cut(s) 151
HaeIII GGCC 6 cut(s) 286, 459, 479, 500, 633, 698
HapII CCGG 5 cut(s) 84, 110, 309, 512, 695
HgaI GACGC 2 cut(s) 68, 742
HhaI GCGC 2 cut(s) 150, 366
Hin1I GRCGYC 1 cut(s) 388
Hin1II CATG 2 cut(s) 104, 604
Hin6I GCGC 2 cut(s) 148, 364
HinP1I GCGC 2 cut(s) 148, 364
HinfI GANTC 2 cut(s) 75, 773
HpaII CCGG 5 cut(s) 84, 110, 309, 512, 695
HphI GGTGA 2 cut(s) 223, 768
Hpy166II GTNNAC 3 cut(s) 107, 214, 733
Hpy188I TCNGA 5 cut(s) 178, 556, 572, 667, 676
Hpy188III TCNNGA 3 cut(s) 440, 685, 796
Hpy8I GTNNAC 3 cut(s) 107, 214, 733
Hpy99I CGWCG 3 cut(s) 390, 393, 758
HpyAV CCTTC 2 cut(s) 61, 598
HpyCH4III ACNGT 5 cut(s) 196, 407, 691, 730, 767
HpyCH4IV ACGT 2 cut(s) 222, 388
HpyCH4V TGCA 7 cut(s) 29, 136, 145, 239, 263, 371, 749
HpyF10VI GCNNNNNNNGC 4 cut(s) 142, 154, 269, 377
HpyF3I CTNAG 2 cut(s) 71, 523
HpySE526I ACGT 2 cut(s) 222, 388
Hsp92I GRCGYC 1 cut(s) 388
Hsp92II CATG 2 cut(s) 104, 604
HspAI GCGC 2 cut(s) 148, 364
KpnI GGTACC 1 cut(s) 695
Ksp22I TGATCA 1 cut(s) 537
Kzo9I GATC 7 cut(s) 163, 248, 280, 537, 616, 667, 681
LguI GCTCTTC 1 cut(s) 341
LmnI GCTCC 1 cut(s) 277
Lsp1109I GCAGC 4 cut(s) 102, 157, 223, 789
LweI GCATC 1 cut(s) 789
MaeI CTAG 2 cut(s) 21, 470
MaeII ACGT 2 cut(s) 222, 388
MaeIII GTNAC 3 cut(s) 96, 401, 407
MalI GATC 7 cut(s) 165, 250, 282, 539, 618, 669, 683
MboI GATC 7 cut(s) 163, 248, 280, 537, 616, 667, 681
MboII GAAGA 7 cut(s) 4, 140, 328, 386, 439, 620, 689
MflI RGATCY 1 cut(s) 681
MluCI AATT 1 cut(s) 576
MluI ACGCGT 1 cut(s) 751
MlyI GAGTC 1 cut(s) 69
MmeI TCCRAC 1 cut(s) 595
MnlI CCTC 4 cut(s) 297, 469, 574, 623
MseI TTAA 1 cut(s) 624
MslI CAYNNNNRTG 3 cut(s) 194, 545, 599
MspCI CTTAAG 1 cut(s) 623
MspI CCGG 5 cut(s) 84, 110, 309, 512, 695
MspR9I CCNGG 1 cut(s) 309
Mva1269I GAATGC 1 cut(s) 31
MvnI CGCG 4 cut(s) 364, 366, 753, 797
MwoI GCNNNNNNNGC 4 cut(s) 142, 154, 269, 377
NciI CCSGG 1 cut(s) 309
NdeII GATC 7 cut(s) 163, 248, 280, 537, 616, 667, 681
NlaIII CATG 2 cut(s) 104, 604
NlaIV GGNNCC 6 cut(s) 313, 324, 346, 499, 569, 693
NmuCI GTSAC 2 cut(s) 96, 407
NruI TCGCGA 1 cut(s) 797
PaqCI CACCTGC 1 cut(s) 230
PceI AGGCCT 1 cut(s) 633
PciSI GCTCTTC 1 cut(s) 341
PcsI WCGNNNNNNNCGW 1 cut(s) 391
PctI GAATGC 1 cut(s) 31
PfeI GAWTC 1 cut(s) 773
PinAI ACCGGT 1 cut(s) 109
PkrI GCNGC 6 cut(s) 117, 147, 238, 461, 562, 804
Ple19I CGATCG 1 cut(s) 283
PleI GAGTC 1 cut(s) 69
PpsI GAGTC 1 cut(s) 69
Ppu21I YACGTR 1 cut(s) 223
PspEI GGTNACC 1 cut(s) 401
PspN4I GGNNCC 6 cut(s) 313, 324, 346, 499, 569, 693
PspPI GGNCC 4 cut(s) 457, 498, 589, 730
PstI CTGCAG 1 cut(s) 241
PsuI RGATCY 1 cut(s) 681
PvuI CGATCG 1 cut(s) 283
RruI TCGCGA 1 cut(s) 797
RsaI GTAC 3 cut(s) 384, 614, 693
RsaNI GTAC 3 cut(s) 383, 613, 692
RseI CAYNNNNRTG 3 cut(s) 194, 545, 599
SapI GCTCTTC 1 cut(s) 341
SaqAI TTAA 1 cut(s) 624
SatI GCNGC 6 cut(s) 116, 146, 237, 460, 561, 803
Sau3AI GATC 7 cut(s) 163, 248, 280, 537, 616, 667, 681
Sau96I GGNCC 4 cut(s) 457, 498, 589, 730
SchI GAGTC 1 cut(s) 69
ScrFI CCNGG 1 cut(s) 309
SetI ASST 7 cut(s) 53, 225, 244, 350, 391, 436, 569
SfaNI GCATC 1 cut(s) 789
SfcI CTRYAG 2 cut(s) 237, 808
SinI GGWCC 2 cut(s) 589, 730
SmiMI CAYNNNNRTG 3 cut(s) 194, 545, 599
SmlI CTYRAG 2 cut(s) 440, 623
SmoI CTYRAG 2 cut(s) 440, 623
Sse9I AATT 1 cut(s) 576
SseBI AGGCCT 1 cut(s) 633
SsiI CCGC 5 cut(s) 366, 460, 493, 560, 592
SspMI CTAG 2 cut(s) 21, 470
StuI AGGCCT 1 cut(s) 633
StyD4I CCNGG 1 cut(s) 307
TaaI ACNGT 5 cut(s) 196, 407, 691, 730, 767
TaiI ACGT 2 cut(s) 225, 391
TaqI TCGA 2 cut(s) 247, 756
TasI AATT 1 cut(s) 576
TauI GCSGC 2 cut(s) 462, 563
TfiI GAWTC 1 cut(s) 773
Tru1I TTAA 1 cut(s) 624
Tru9I TTAA 1 cut(s) 624
TscAI CASTG 2 cut(s) 645, 772
TseFI GTSAC 2 cut(s) 96, 407
TseI GCWGC 4 cut(s) 115, 145, 236, 802
Tsp45I GTSAC 2 cut(s) 96, 407
TspDTI ATGAA 2 cut(s) 43, 617
TspRI CASTG 2 cut(s) 645, 772
Vha464I CTTAAG 1 cut(s) 623
VpaK11BI GGWCC 2 cut(s) 589, 730
XmiI GTMKAC 1 cut(s) 106
XspI CTAG 2 cut(s) 21, 470
ZraI GACGTC 1 cut(s) 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.