MD06G1085400.v1.1

rRNA N-glycosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0018098
Physical Location & Seq
Reverse (-)
20912763 .. 20913626
864 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1085400.v1.1.491

Sequence Viewer

Length: 843 bp
ATGGCACTTGCCCTCTCTCTTTTGAATGCAACCCCTAAGACATACACAGCATTCATCGAAGCACTACGAGCCCGACTCACAGCCGGACGACCCACATCACATGGTATACCGGTGCTGCCCCGAAGAGAAGACGTGTCAGACGCGCAGCGCTTTCTTTATGTTGATCTCACCAACTATAACGGCGACACCATCAGGGTCGCCATCGATGTAGTAAACGTTTATGTGGTGGGATATCGTTCTGGTAATAAATCCTACATACTTGCAAATAACGCTGAAAATCCGGCTCCAACGCACATATTATTCCCCACCGCCCCCGGCGCCGCGCAGTCCACGCGGACTATGCTGCCCTTCACCGGAGACTACCCCGCACTTGGAGCATATGCACGACGTACAGCGCAACCGAGTGCATCAGGTGCTCGTAACCCAGGGTCACGTATACATGAGGATATACCTATGCTTGAGCAAATTCCTCTGGGACGCAATGAACTCGATAACGCCATCAGTAAGTTGCACTATGCGGCATCCCACTCAGACCAAGCCGCGGCGTTTATTGTGATCATTCAGATGGTCTCGGAAGCGGCAAGGTTCCGGTACATTGAGAGTCAAGTCGGCACTAGAATGGGGATAGACAACCCTCCTTATATACCAGACCCTGCAATGCGGAGCCTGGAAACCAATTGGTCTGCTCTCTCGGAGCAGATCCAGAAGGTACCAGCCAATGGAAAACGCTTCAATAGGACTATTCAGCTCACTACTGTCAACAATCGTCCCTTCGTAGTTGACAGTGTTGAAGCAGACATGGTTCAGCGACGGGGCATCGCGATGCTCCTCTACGCGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RIP

Protein Analysis

281

Amino Acids

30.83

Weight (kDa)

9.48

Isoelectric Point (pI)

41.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RIP PF00161 11 - 235 6.1e-42 Ribosome inactivating protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 709
AccB1I GGYRCC 2 cut(s) 317, 709
AccB7I CCANNNNNTGG 1 cut(s) 719
AccI GTMKAC 2 cut(s) 106, 436
AccII CGCG 6 cut(s) 143, 323, 334, 542, 821, 836
AciI CCGC 9 cut(s) 309, 321, 334, 366, 518, 540, 542, 578, 661
AclI AACGTT 1 cut(s) 216
AclWI GGATC 1 cut(s) 694
AcsI RAATTY 1 cut(s) 465
AcyI GRCGYC 1 cut(s) 318
AfaI GTAC 3 cut(s) 391, 593, 711
AfeI AGCGCT 1 cut(s) 149
AfiI CCNNNNNNNGG 4 cut(s) 353, 371, 541, 719
AflIII ACRYGT 1 cut(s) 132
AgeI ACCGGT 1 cut(s) 109
AgsI TTSAA 3 cut(s) 25, 733, 791
AjiI CACGTC 1 cut(s) 133
AjnI CCWGG 2 cut(s) 424, 666
AluBI AGCT 1 cut(s) 748
AluI AGCT 1 cut(s) 748
Alw21I GWGCWC 1 cut(s) 418
Alw26I GTCTC 2 cut(s) 351, 574
AlwI GGATC 1 cut(s) 694
AlwNI CAGNNNCTG 1 cut(s) 653
Aor51HI AGCGCT 1 cut(s) 149
ApeKI GCWGC 3 cut(s) 115, 145, 343
ApoI RAATTY 1 cut(s) 465
AsiGI ACCGGT 1 cut(s) 109
Asp718I GGTACC 1 cut(s) 709
AspLEI GCGC 5 cut(s) 145, 150, 320, 325, 397
AsuC2I CCSGG 1 cut(s) 315
AsuHPI GGTGA 2 cut(s) 160, 343
BanI GGYRCC 2 cut(s) 317, 709
BanII GRGCYC 1 cut(s) 73
BbsI GAAGAC 1 cut(s) 135
Bbv12I GWGCWC 1 cut(s) 418
BbvI GCAGC 3 cut(s) 102, 157, 330
BccI CCATC 4 cut(s) 197, 209, 506, 559
BceAI ACGGC 1 cut(s) 196
BcgI CGANNNNNNTGC 2 cut(s) 469, 503
BciT130I CCWGG 2 cut(s) 426, 668
BclI TGATCA 1 cut(s) 555
BcnI CCSGG 1 cut(s) 315
BcoDI GTCTC 2 cut(s) 351, 574
BfaI CTAG 1 cut(s) 615
BfoI RGCGCY 2 cut(s) 151, 321
BisI GCNGC 8 cut(s) 116, 146, 321, 344, 519, 540, 543, 579
BlsI GCNGC 8 cut(s) 117, 147, 322, 345, 520, 541, 544, 580
Bme1390I CCNGG 3 cut(s) 315, 426, 668
BmgBI CACGTC 1 cut(s) 133
BmiI GGNNCC 5 cut(s) 285, 319, 587, 665, 711
BmrFI CCNGG 3 cut(s) 315, 426, 668
BmsI GCATC 4 cut(s) 416, 530, 813, 825
BpiI GAAGAC 1 cut(s) 135
BplI GAGNNNNNCTC 2 cut(s) 60, 92
BpuEI CTTGAG 1 cut(s) 479
BpuMI CCSGG 1 cut(s) 315
Bsa29I ATCGAT 1 cut(s) 204
BsaAI YACGTR 1 cut(s) 434
BsaHI GRCGYC 1 cut(s) 318
BsaI GGTCTC 1 cut(s) 574
BsaJI CCNNGG 4 cut(s) 313, 424, 425, 540
BsaWI WCCGGW 3 cut(s) 109, 353, 588
Bsc4I CCNNNNNNNGG 4 cut(s) 353, 371, 541, 719
Bse118I RCCGGY 1 cut(s) 109
Bse3DI GCAATG 2 cut(s) 487, 663
BseBI CCWGG 2 cut(s) 426, 668
BseCI ATCGAT 1 cut(s) 204
BseDI CCNNGG 4 cut(s) 313, 424, 425, 540
BseGI GGATG 1 cut(s) 521
BseLI CCNNNNNNNGG 4 cut(s) 353, 371, 541, 719
BseMI GCAATG 2 cut(s) 487, 663
BseMII CTCAG 1 cut(s) 543
BseRI GAGGAG 1 cut(s) 818
BseXI GCAGC 3 cut(s) 102, 157, 330
Bsh1236I CGCG 6 cut(s) 143, 323, 334, 542, 821, 836
BshNI GGYRCC 2 cut(s) 317, 709
BshTI ACCGGT 1 cut(s) 109
BshVI ATCGAT 1 cut(s) 204
BsiHKAI GWGCWC 1 cut(s) 418
BsiSI CCGG 6 cut(s) 84, 110, 281, 315, 354, 589
BslFI GGGAC 2 cut(s) 489, 753
BslI CCNNNNNNNGG 4 cut(s) 353, 371, 541, 719
BsmAI GTCTC 2 cut(s) 351, 574
BsmFI GGGAC 2 cut(s) 489, 753
BsmI GAATGC 2 cut(s) 31, 50
Bso31I GGTCTC 1 cut(s) 574
Bsp1286I GDGCHC 2 cut(s) 73, 418
Bsp143I GATC 3 cut(s) 163, 555, 699
Bsp68I TCGCGA 1 cut(s) 821
BspACI CCGC 9 cut(s) 309, 321, 334, 366, 518, 540, 542, 578, 661
BspCNI CTCAG 1 cut(s) 542
BspDI ATCGAT 1 cut(s) 204
BspFNI CGCG 6 cut(s) 143, 323, 334, 542, 821, 836
BspLI GGNNCC 5 cut(s) 285, 319, 587, 665, 711
BspPI GGATC 1 cut(s) 694
BspT107I GGYRCC 2 cut(s) 317, 709
BspTNI GGTCTC 1 cut(s) 574
BsrDI GCAATG 2 cut(s) 487, 663
BsrFI RCCGGY 1 cut(s) 109
BssAI RCCGGY 1 cut(s) 109
BssECI CCNNGG 4 cut(s) 313, 424, 425, 540
BssMI GATC 3 cut(s) 163, 555, 699
BssNAI GTATAC 2 cut(s) 107, 437
BssNI GRCGYC 1 cut(s) 318
Bst1107I GTATAC 2 cut(s) 107, 437
Bst2UI CCWGG 2 cut(s) 426, 668
Bst4CI ACNGT 2 cut(s) 757, 785
Bst6I CTCTTC 1 cut(s) 118
BstACI GRCGYC 1 cut(s) 318
BstAPI GCANNNNNTGC 1 cut(s) 413
BstBAI YACGTR 1 cut(s) 434
BstDEI CTNAG 2 cut(s) 36, 529
BstDSI CCRYGG 1 cut(s) 540
BstF5I GGATG 1 cut(s) 521
BstFNI CGCG 6 cut(s) 143, 323, 334, 542, 821, 836
BstH2I RGCGCY 2 cut(s) 151, 321
BstHHI GCGC 5 cut(s) 145, 150, 320, 325, 397
BstKTI GATC 3 cut(s) 166, 558, 702
BstMAI GTCTC 2 cut(s) 351, 574
BstMBI GATC 3 cut(s) 163, 555, 699
BstMWI GCNNNNNNNGC 7 cut(s) 68, 269, 317, 331, 340, 374, 413
BstNI CCWGG 2 cut(s) 426, 668
BstSCI CCNGG 3 cut(s) 313, 424, 666
BstUI CGCG 6 cut(s) 143, 323, 334, 542, 821, 836
BstV1I GCAGC 3 cut(s) 102, 157, 330
BstV2I GAAGAC 1 cut(s) 135
BstX2I RGATCY 1 cut(s) 699
BstYI RGATCY 1 cut(s) 699
BstZ17I GTATAC 2 cut(s) 107, 437
Bsu15I ATCGAT 1 cut(s) 204
BsuTUI ATCGAT 1 cut(s) 204
BtgI CCRYGG 1 cut(s) 540
BtgZI GCGATG 2 cut(s) 802, 836
BtrI CACGTC 1 cut(s) 133
BtsCI GGATG 1 cut(s) 521
BtsIMutI CAGTG 1 cut(s) 790
BtuMI TCGCGA 1 cut(s) 821
CaiI CAGNNNCTG 1 cut(s) 653
CfoI GCGC 5 cut(s) 145, 150, 320, 325, 397
Cfr10I RCCGGY 1 cut(s) 109
Cfr42I CCGCGG 1 cut(s) 543
ClaI ATCGAT 1 cut(s) 204
CseI GACGC 2 cut(s) 149, 486
Csp6I GTAC 3 cut(s) 390, 592, 710
CspAI ACCGGT 1 cut(s) 109
CviAII CATG 3 cut(s) 101, 440, 799
CviJI RGCY 7 cut(s) 71, 83, 284, 539, 666, 716, 748
CviKI_1 RGCY 7 cut(s) 71, 83, 284, 539, 666, 716, 748
CviQI GTAC 3 cut(s) 390, 592, 710
DdeI CTNAG 2 cut(s) 36, 529
DinI GGCGCC 1 cut(s) 319
DpnI GATC 3 cut(s) 165, 557, 701
DpnII GATC 3 cut(s) 163, 555, 699
Eam1104I CTCTTC 1 cut(s) 118
EarI CTCTTC 1 cut(s) 118
Eco24I GRGCYC 1 cut(s) 73
Eco31I GGTCTC 1 cut(s) 574
Eco32I GATATC 1 cut(s) 233
Eco47III AGCGCT 1 cut(s) 149
EcoRII CCWGG 2 cut(s) 424, 666
EcoRV GATATC 1 cut(s) 233
EcoT38I GRGCYC 1 cut(s) 73
EgeI GGCGCC 1 cut(s) 319
EheI GGCGCC 1 cut(s) 319
FaeI CATG 3 cut(s) 104, 443, 802
FaqI GGGAC 2 cut(s) 489, 753
FatI CATG 3 cut(s) 100, 439, 798
FauI CCCGC 1 cut(s) 373
FauNDI CATATG 1 cut(s) 379
FbaI TGATCA 1 cut(s) 555
FblI GTMKAC 2 cut(s) 106, 436
Fnu4HI GCNGC 8 cut(s) 116, 146, 321, 344, 519, 540, 543, 579
FokI GGATG 1 cut(s) 508
FriOI GRGCYC 1 cut(s) 73
Fsp4HI GCNGC 8 cut(s) 116, 146, 321, 344, 519, 540, 543, 579
FspBI CTAG 1 cut(s) 615
GlaI GCGC 5 cut(s) 144, 149, 319, 324, 396
GluI GCNGC 8 cut(s) 116, 146, 321, 344, 519, 540, 543, 579
HaeII RGCGCY 2 cut(s) 151, 321
HapII CCGG 6 cut(s) 84, 110, 281, 315, 354, 589
HgaI GACGC 2 cut(s) 149, 486
HhaI GCGC 5 cut(s) 145, 150, 320, 325, 397
Hin1I GRCGYC 1 cut(s) 318
Hin1II CATG 3 cut(s) 104, 443, 802
Hin6I GCGC 5 cut(s) 143, 148, 318, 323, 395
HinP1I GCGC 5 cut(s) 143, 148, 318, 323, 395
HincII GTYRAC 2 cut(s) 760, 781
HindII GTYRAC 2 cut(s) 760, 781
HinfI GANTC 2 cut(s) 75, 601
HpaII CCGG 6 cut(s) 84, 110, 281, 315, 354, 589
HphI GGTGA 2 cut(s) 160, 343
Hpy166II GTNNAC 6 cut(s) 107, 214, 330, 437, 760, 781
Hpy188I TCNGA 5 cut(s) 139, 532, 564, 574, 694
Hpy188III TCNNGA 2 cut(s) 703, 820
Hpy8I GTNNAC 6 cut(s) 107, 214, 330, 437, 760, 781
Hpy99I CGWCG 2 cut(s) 390, 813
HpyAV CCTTC 3 cut(s) 358, 700, 781
HpyCH4III ACNGT 2 cut(s) 757, 785
HpyCH4IV ACGT 4 cut(s) 132, 216, 388, 433
HpyCH4V TGCA 6 cut(s) 29, 263, 383, 407, 511, 656
HpyF10VI GCNNNNNNNGC 7 cut(s) 68, 269, 317, 331, 340, 374, 413
HpyF3I CTNAG 2 cut(s) 36, 529
HpySE526I ACGT 4 cut(s) 132, 216, 388, 433
Hsp92I GRCGYC 1 cut(s) 318
Hsp92II CATG 3 cut(s) 104, 443, 802
HspAI GCGC 5 cut(s) 143, 148, 318, 323, 395
KasI GGCGCC 1 cut(s) 317
KpnI GGTACC 1 cut(s) 713
Ksp22I TGATCA 1 cut(s) 555
KspI CCGCGG 1 cut(s) 543
Kzo9I GATC 3 cut(s) 163, 555, 699
LmnI GCTCC 5 cut(s) 289, 374, 663, 694, 831
Lsp1109I GCAGC 3 cut(s) 102, 157, 330
LweI GCATC 4 cut(s) 416, 530, 813, 825
MaeI CTAG 1 cut(s) 615
MaeII ACGT 4 cut(s) 132, 216, 388, 433
MaeIII GTNAC 2 cut(s) 419, 429
MalI GATC 3 cut(s) 165, 557, 701
MboI GATC 3 cut(s) 163, 555, 699
MboII GAAGA 2 cut(s) 135, 140
MfeI CAATTG 1 cut(s) 676
MflI RGATCY 1 cut(s) 699
MhlI GDGCHC 2 cut(s) 73, 418
MluCI AATT 2 cut(s) 465, 676
Mly113I GGCGCC 1 cut(s) 318
MlyI GAGTC 2 cut(s) 69, 610
MmeI TCCRAC 1 cut(s) 311
MnlI CCTC 5 cut(s) 23, 436, 480, 645, 839
MslI CAYNNNNRTG 3 cut(s) 563, 617, 821
MspA1I CMGCKG 1 cut(s) 542
MspI CCGG 6 cut(s) 84, 110, 281, 315, 354, 589
MspR9I CCNGG 3 cut(s) 315, 426, 668
MteI GCGCNGCGC 2 cut(s) 146, 321
MunI CAATTG 1 cut(s) 676
Mva1269I GAATGC 2 cut(s) 31, 50
MvaI CCWGG 2 cut(s) 426, 668
MvnI CGCG 6 cut(s) 143, 323, 334, 542, 821, 836
MwoI GCNNNNNNNGC 7 cut(s) 68, 269, 317, 331, 340, 374, 413
NarI GGCGCC 1 cut(s) 318
NciI CCSGG 1 cut(s) 315
NdeI CATATG 1 cut(s) 379
NdeII GATC 3 cut(s) 163, 555, 699
NlaIII CATG 3 cut(s) 104, 443, 802
NlaIV GGNNCC 5 cut(s) 285, 319, 587, 665, 711
NmuCI GTSAC 1 cut(s) 429
NruI TCGCGA 1 cut(s) 821
PasI CCCWGGG 1 cut(s) 425
PctI GAATGC 2 cut(s) 31, 50
PflMI CCANNNNNTGG 1 cut(s) 719
PinAI ACCGGT 1 cut(s) 109
PkrI GCNGC 8 cut(s) 117, 147, 322, 345, 520, 541, 544, 580
PleI GAGTC 2 cut(s) 69, 609
PluTI GGCGCC 1 cut(s) 321
PpsI GAGTC 2 cut(s) 69, 609
Ppu21I YACGTR 1 cut(s) 434
Psp1406I AACGTT 1 cut(s) 216
Psp6I CCWGG 2 cut(s) 424, 666
PspGI CCWGG 2 cut(s) 424, 666
PspN4I GGNNCC 5 cut(s) 285, 319, 587, 665, 711
PstNI CAGNNNCTG 1 cut(s) 653
PsuI RGATCY 1 cut(s) 699
RruI TCGCGA 1 cut(s) 821
RsaI GTAC 3 cut(s) 391, 593, 711
RsaNI GTAC 3 cut(s) 390, 592, 710
RseI CAYNNNNRTG 3 cut(s) 563, 617, 821
SacII CCGCGG 1 cut(s) 543
SatI GCNGC 8 cut(s) 116, 146, 321, 344, 519, 540, 543, 579
Sau3AI GATC 3 cut(s) 163, 555, 699
SchI GAGTC 2 cut(s) 69, 610
ScrFI CCNGG 3 cut(s) 315, 426, 668
SduI GDGCHC 2 cut(s) 73, 418
SetI ASST 9 cut(s) 135, 219, 391, 415, 436, 454, 587, 711, 750
SfaNI GCATC 4 cut(s) 416, 530, 813, 825
SfoI GGCGCC 1 cut(s) 319
Sfr303I CCGCGG 1 cut(s) 543
SgrBI CCGCGG 1 cut(s) 543
SmiMI CAYNNNNRTG 3 cut(s) 563, 617, 821
SmlI CTYRAG 1 cut(s) 458
SmoI CTYRAG 1 cut(s) 458
Sse9I AATT 2 cut(s) 465, 676
SsiI CCGC 9 cut(s) 309, 321, 334, 366, 518, 540, 542, 578, 661
SspDI GGCGCC 1 cut(s) 317
SspMI CTAG 1 cut(s) 615
StyD4I CCNGG 3 cut(s) 313, 424, 666
TaaI ACNGT 2 cut(s) 757, 785
TaiI ACGT 4 cut(s) 135, 219, 391, 436
TaqI TCGA 3 cut(s) 57, 204, 489
TasI AATT 2 cut(s) 465, 676
TauI GCSGC 5 cut(s) 323, 521, 542, 545, 581
TscAI CASTG 1 cut(s) 790
TseFI GTSAC 1 cut(s) 429
TseI GCWGC 3 cut(s) 115, 145, 343
Tsp45I GTSAC 1 cut(s) 429
TspDTI ATGAA 2 cut(s) 43, 498
TspRI CASTG 1 cut(s) 790
Van91I CCANNNNNTGG 1 cut(s) 719
XapI RAATTY 1 cut(s) 465
XmiI GTMKAC 2 cut(s) 106, 436
XspI CTAG 1 cut(s) 615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.