Prupe.1G294500_v2.0.a1

rRNA N-glycosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0018098
Physical Location & Seq
Forward (+)
29174283 .. 29175519
1237 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G294500.1

Sequence Viewer

Length: 915 bp
ATGGCACTGTCCTTCTCCACTAAGAATACAACCCCCCAGAAATACCGCGACTTCATAGAATCTCTGCGACAGCGACTCACAGCCGGACGATCTAATTCACACGGTATACCGGTGCTACCTAGAAGAGAAGATGTGCCAGACGCACAGCGCTTTCTTCTTGTTGATCTCACAAACTCTGGAAACAATACCATCAAGCTCGCAATCGATGTGGTGAACGCTTACGTGGTGGGTTATGCTGCAGGTGGCCGATCCTACTTTCTTAAAGAAAATGCTCGTGAGAATCCGCCTCCAATCCACACCTTGTTCAGCGACACCACTCGGATGCCTCCTCTAGACTTCGACGGCAGCTACACCGGTCTTTCGCGAGCTGCGCAAGAAGCAGTGAAACGGAATATAGCACGTGATCGTGCTCGTAACCCAGCAGTGGCAGATCTGCATCCAGATACATCTATTCTTGAGCGGATACCTCTGGGACGCAATGAACTAGATGACGCCATCAACCTGTTGCGCTTGGCACCATCCCAGTCCGACCAAGCTGTAGGGTTTATAGTGGTAATTCAGATGATCTGCGAAGCGGCAAGGTTTCGGTTCATTGAGGGTCTAATCCGCAATAGCATGAAGGATGTCTACGATCCCATTATACCAGGCCTTGCAATTCGGAGCCTGGAAACCCATTGGTCTGATCTCTCTGAAGAGATCCAGAGGGTTCCGGCCAATCAAACACCATTCCAGAAGGCTGTTGTGCTCCATAATATCAAAAATGAACGCGTTGAAGTACGCAGTGTTGACTCAGACGTTGTTCGGGGTGTCGCTATGCTGCTCTACGATCGAAACCAAAATGCCAATCCTGGACCTTCAACGAAAAAGCCTCTGTTAAAGAATCTAAAGCCTCACATCGGAAAACCCAAAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RIP

Protein Analysis

305

Amino Acids

33.99

Weight (kDa)

9.58

Isoelectric Point (pI)

40.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 230
Acc16I TGCGCA 1 cut(s) 372
Acc36I ACCTGC 1 cut(s) 230
AccB1I GGYRCC 1 cut(s) 514
AccBSI CCGCTC 1 cut(s) 460
AccI GTMKAC 2 cut(s) 106, 627
AccII CGCG 3 cut(s) 48, 364, 768
AciI CCGC 5 cut(s) 46, 284, 460, 575, 607
AclWI GGATC 3 cut(s) 243, 626, 691
AcoI YGGCCR 2 cut(s) 244, 711
AcuI CTGAAG 1 cut(s) 711
AcvI CACGTG 1 cut(s) 401
AcyI GRCGYC 1 cut(s) 492
AfaI GTAC 1 cut(s) 777
AfeI AGCGCT 1 cut(s) 149
AfiI CCNNNNNNNGG 2 cut(s) 424, 896
AflIII ACRYGT 1 cut(s) 766
AgeI ACCGGT 2 cut(s) 109, 353
AgsI TTSAA 2 cut(s) 773, 858
AjnI CCWGG 3 cut(s) 643, 663, 847
AluBI AGCT 4 cut(s) 196, 348, 368, 536
AluI AGCT 4 cut(s) 196, 348, 368, 536
Alw21I GWGCWC 2 cut(s) 412, 747
AlwI GGATC 3 cut(s) 243, 626, 691
Aor51HI AGCGCT 1 cut(s) 149
AoxI GGCC 3 cut(s) 244, 646, 711
ApeKI GCWGC 4 cut(s) 236, 345, 368, 817
AsiGI ACCGGT 2 cut(s) 109, 353
AspLEI GCGC 3 cut(s) 150, 373, 510
AspS9I GGNCC 1 cut(s) 851
AsuHPI GGTGA 1 cut(s) 223
AvaII GGWCC 1 cut(s) 851
BanI GGYRCC 1 cut(s) 514
BarI GAAGNNNNNNTAC 2 cut(s) 611, 643
BauI CACGAG 1 cut(s) 273
BbrPI CACGTG 1 cut(s) 401
Bbv12I GWGCWC 2 cut(s) 412, 747
BbvI GCAGC 4 cut(s) 223, 355, 357, 804
BccI CCATC 3 cut(s) 197, 503, 526
BceAI ACGGC 1 cut(s) 358
BciT130I CCWGG 3 cut(s) 645, 665, 849
BciVI GTATCC 1 cut(s) 456
BfaI CTAG 3 cut(s) 120, 332, 485
BfmI CTRYAG 2 cut(s) 237, 537
BfoI RGCGCY 1 cut(s) 151
BfuAI ACCTGC 1 cut(s) 230
BfuI GTATCC 1 cut(s) 456
BglII AGATCT 1 cut(s) 430
BisI GCNGC 5 cut(s) 237, 346, 369, 576, 818
BlsI GCNGC 5 cut(s) 238, 347, 370, 577, 819
Bme1390I CCNGG 3 cut(s) 645, 665, 849
Bme18I GGWCC 1 cut(s) 851
BmgT120I GGNCC 1 cut(s) 851
BmiI GGNNCC 3 cut(s) 516, 662, 708
BmrFI CCNGG 3 cut(s) 645, 665, 849
BmrI ACTGGG 1 cut(s) 517
BmsI GCATC 2 cut(s) 312, 445
BmuI ACTGGG 1 cut(s) 517
BpuEI CTTGAG 1 cut(s) 476
Bsa29I ATCGAT 1 cut(s) 204
BsaAI YACGTR 2 cut(s) 223, 401
BsaBI GATNNNNATC 1 cut(s) 435
BsaHI GRCGYC 1 cut(s) 492
BsaWI WCCGGW 2 cut(s) 109, 353
Bsc4I CCNNNNNNNGG 2 cut(s) 424, 896
Bse118I RCCGGY 2 cut(s) 109, 353
Bse1I ACTGG 1 cut(s) 523
Bse3DI GCAATG 1 cut(s) 484
Bse8I GATNNNNATC 1 cut(s) 435
BseBI CCWGG 3 cut(s) 645, 665, 849
BseCI ATCGAT 1 cut(s) 204
BseGI GGATG 4 cut(s) 327, 436, 518, 628
BseJI GATNNNNATC 1 cut(s) 435
BseLI CCNNNNNNNGG 2 cut(s) 424, 896
BseMI GCAATG 1 cut(s) 484
BseMII CTCAG 1 cut(s) 804
BseNI ACTGG 1 cut(s) 523
BseRI GAGGAG 1 cut(s) 318
BseXI GCAGC 4 cut(s) 223, 355, 357, 804
BseYI CCCAGC 1 cut(s) 418
Bsh1236I CGCG 3 cut(s) 48, 364, 768
Bsh1285I CGRYCG 1 cut(s) 829
BshFI GGCC 3 cut(s) 246, 648, 713
BshNI GGYRCC 1 cut(s) 514
BshTI ACCGGT 2 cut(s) 109, 353
BshVI ATCGAT 1 cut(s) 204
BsiEI CGRYCG 1 cut(s) 829
BsiHKAI GWGCWC 2 cut(s) 412, 747
BsiSI CCGG 4 cut(s) 84, 110, 354, 710
BslFI GGGAC 1 cut(s) 486
BslI CCNNNNNNNGG 2 cut(s) 424, 896
BsmFI GGGAC 1 cut(s) 486
BsnI GGCC 3 cut(s) 246, 648, 713
Bsp1286I GDGCHC 2 cut(s) 412, 747
Bsp68I TCGCGA 1 cut(s) 364
BspACI CCGC 5 cut(s) 46, 284, 460, 575, 607
BspANI GGCC 3 cut(s) 246, 648, 713
BspCNI CTCAG 1 cut(s) 803
BspDI ATCGAT 1 cut(s) 204
BspFNI CGCG 3 cut(s) 48, 364, 768
BspLI GGNNCC 3 cut(s) 516, 662, 708
BspMAI CTGCAG 1 cut(s) 241
BspMI ACCTGC 1 cut(s) 230
BspPI GGATC 3 cut(s) 243, 626, 691
BspT107I GGYRCC 1 cut(s) 514
BsrBI CCGCTC 1 cut(s) 460
BsrDI GCAATG 1 cut(s) 484
BsrFI RCCGGY 2 cut(s) 109, 353
BsrI ACTGG 1 cut(s) 523
BssAI RCCGGY 2 cut(s) 109, 353
BssNAI GTATAC 1 cut(s) 107
BssNI GRCGYC 1 cut(s) 492
BssSI CACGAG 1 cut(s) 273
Bst1107I GTATAC 1 cut(s) 107
Bst2BI CACGAG 1 cut(s) 273
Bst2UI CCWGG 3 cut(s) 645, 665, 849
Bst4CI ACNGT 2 cut(s) 9, 104
Bst6I CTCTTC 2 cut(s) 118, 687
BstACI GRCGYC 1 cut(s) 492
BstBAI YACGTR 2 cut(s) 223, 401
BstC8I GCNNGC 2 cut(s) 198, 366
BstDEI CTNAG 2 cut(s) 21, 790
BstF5I GGATG 4 cut(s) 327, 436, 518, 628
BstFNI CGCG 3 cut(s) 48, 364, 768
BstH2I RGCGCY 1 cut(s) 151
BstHHI GCGC 3 cut(s) 150, 373, 510
BstMCI CGRYCG 1 cut(s) 829
BstMWI GCNNNNNNNGC 2 cut(s) 370, 377
BstNI CCWGG 3 cut(s) 645, 665, 849
BstSCI CCNGG 3 cut(s) 643, 663, 847
BstSFI CTRYAG 2 cut(s) 237, 537
BstUI CGCG 3 cut(s) 48, 364, 768
BstV1I GCAGC 4 cut(s) 223, 355, 357, 804
BstX2I RGATCY 2 cut(s) 430, 696
BstYI RGATCY 2 cut(s) 430, 696
BstZ17I GTATAC 1 cut(s) 107
Bsu15I ATCGAT 1 cut(s) 204
BsuI GTATCC 1 cut(s) 456
BsuRI GGCC 3 cut(s) 246, 648, 713
BsuTUI ATCGAT 1 cut(s) 204
BtsCI GGATG 4 cut(s) 327, 436, 518, 628
BtsI GCAGTG 3 cut(s) 387, 429, 787
BtsIMutI CAGTG 4 cut(s) 5, 387, 429, 787
BtuMI TCGCGA 1 cut(s) 364
BveI ACCTGC 1 cut(s) 230
Cac8I GCNNGC 2 cut(s) 198, 366
CfoI GCGC 3 cut(s) 150, 373, 510
Cfr10I RCCGGY 2 cut(s) 109, 353
Cfr13I GGNCC 1 cut(s) 851
ClaI ATCGAT 1 cut(s) 204
CseI GACGC 3 cut(s) 149, 483, 500
Csp6I GTAC 1 cut(s) 776
CspAI ACCGGT 2 cut(s) 109, 353
CspCI CAANNNNNGTGG 2 cut(s) 189, 224
CviAII CATG 1 cut(s) 616
CviQI GTAC 1 cut(s) 776
DdeI CTNAG 2 cut(s) 21, 790
EaeI YGGCCR 2 cut(s) 244, 711
Eam1104I CTCTTC 2 cut(s) 118, 687
EarI CTCTTC 2 cut(s) 118, 687
EciI GGCGGA 1 cut(s) 273
Eco147I AGGCCT 1 cut(s) 648
Eco47I GGWCC 1 cut(s) 851
Eco47III AGCGCT 1 cut(s) 149
Eco57I CTGAAG 1 cut(s) 711
Eco72I CACGTG 1 cut(s) 401
EcoRII CCWGG 3 cut(s) 643, 663, 847
FaeI CATG 1 cut(s) 619
FaiI YATR 9 cut(s) 56, 107, 234, 395, 548, 617, 641, 750, 815
FaqI GGGAC 1 cut(s) 486
FatI CATG 1 cut(s) 615
FblI GTMKAC 2 cut(s) 106, 627
Fnu4HI GCNGC 5 cut(s) 237, 346, 369, 576, 818
FokI GGATG 4 cut(s) 334, 423, 505, 635
Fsp4HI GCNGC 5 cut(s) 237, 346, 369, 576, 818
FspBI CTAG 3 cut(s) 120, 332, 485
FspI TGCGCA 1 cut(s) 372
GlaI GCGC 3 cut(s) 149, 372, 509
GluI GCNGC 5 cut(s) 237, 346, 369, 576, 818
GsaI CCCAGC 1 cut(s) 422
HaeII RGCGCY 1 cut(s) 151
HaeIII GGCC 3 cut(s) 246, 648, 713
HapII CCGG 4 cut(s) 84, 110, 354, 710
HgaI GACGC 3 cut(s) 149, 483, 500
HhaI GCGC 3 cut(s) 150, 373, 510
Hin1I GRCGYC 1 cut(s) 492
Hin1II CATG 1 cut(s) 619
Hin6I GCGC 3 cut(s) 148, 371, 508
HinP1I GCGC 3 cut(s) 148, 371, 508
HincII GTYRAC 1 cut(s) 787
HindII GTYRAC 1 cut(s) 787
HinfI GANTC 5 cut(s) 59, 75, 280, 788, 880
HpaII CCGG 4 cut(s) 84, 110, 354, 710
HphI GGTGA 1 cut(s) 223
Hpy166II GTNNAC 4 cut(s) 107, 214, 628, 787
Hpy188I TCNGA 8 cut(s) 321, 529, 561, 660, 682, 691, 793, 899
Hpy188III TCNNGA 8 cut(s) 177, 275, 332, 363, 440, 455, 700, 730
Hpy8I GTNNAC 4 cut(s) 107, 214, 628, 787
Hpy99I CGWCG 1 cut(s) 344
HpyAV CCTTC 4 cut(s) 22, 613, 727, 864
HpyCH4III ACNGT 2 cut(s) 9, 104
HpyCH4IV ACGT 3 cut(s) 222, 400, 795
HpyCH4V TGCA 3 cut(s) 239, 436, 653
HpyF10VI GCNNNNNNNGC 2 cut(s) 370, 377
HpyF3I CTNAG 2 cut(s) 21, 790
HpySE526I ACGT 3 cut(s) 222, 400, 795
Hsp92I GRCGYC 1 cut(s) 492
Hsp92II CATG 1 cut(s) 619
HspAI GCGC 3 cut(s) 148, 371, 508
LmnI GCTCC 2 cut(s) 660, 750
Lsp1109I GCAGC 4 cut(s) 223, 355, 357, 804
LweI GCATC 2 cut(s) 312, 445
MaeI CTAG 3 cut(s) 120, 332, 485
MaeII ACGT 3 cut(s) 222, 400, 795
MaeIII GTNAC 1 cut(s) 413
MbiI CCGCTC 1 cut(s) 460
MboII GAAGA 4 cut(s) 135, 140, 146, 704
MflI RGATCY 2 cut(s) 430, 696
MhlI GDGCHC 2 cut(s) 412, 747
MluCI AATT 3 cut(s) 94, 555, 654
MluI ACGCGT 1 cut(s) 766
MlyI GAGTC 2 cut(s) 69, 782
MmeI TCCRAC 1 cut(s) 552
MnlI CCTC 8 cut(s) 297, 336, 339, 477, 589, 696, 879, 900
MseI TTAA 2 cut(s) 261, 875
MslI CAYNNNNRTG 1 cut(s) 320
MspI CCGG 4 cut(s) 84, 110, 354, 710
MspR9I CCNGG 3 cut(s) 645, 665, 849
MvaI CCWGG 3 cut(s) 645, 665, 849
MvnI CGCG 3 cut(s) 48, 364, 768
MwoI GCNNNNNNNGC 2 cut(s) 370, 377
NlaIII CATG 1 cut(s) 619
NlaIV GGNNCC 3 cut(s) 516, 662, 708
NruI TCGCGA 1 cut(s) 364
NsbI TGCGCA 1 cut(s) 372
PaqCI CACCTGC 1 cut(s) 230
PceI AGGCCT 1 cut(s) 648
PfeI GAWTC 3 cut(s) 59, 280, 880
PfoI TCCNGGA 1 cut(s) 847
PinAI ACCGGT 2 cut(s) 109, 353
PkrI GCNGC 5 cut(s) 238, 347, 370, 577, 819
Ple19I CGATCG 1 cut(s) 829
PleI GAGTC 2 cut(s) 69, 782
PmaCI CACGTG 1 cut(s) 401
PmlI CACGTG 1 cut(s) 401
PpsI GAGTC 2 cut(s) 69, 782
Ppu21I YACGTR 2 cut(s) 223, 401
Psp6I CCWGG 3 cut(s) 643, 663, 847
PspCI CACGTG 1 cut(s) 401
PspFI CCCAGC 1 cut(s) 418
PspGI CCWGG 3 cut(s) 643, 663, 847
PspN4I GGNNCC 3 cut(s) 516, 662, 708
PspPI GGNCC 1 cut(s) 851
PstI CTGCAG 1 cut(s) 241
PsuI RGATCY 2 cut(s) 430, 696
PvuI CGATCG 1 cut(s) 829
RruI TCGCGA 1 cut(s) 364
RsaI GTAC 1 cut(s) 777
RsaNI GTAC 1 cut(s) 776
RseI CAYNNNNRTG 1 cut(s) 320
SaqAI TTAA 2 cut(s) 261, 875
SatI GCNGC 5 cut(s) 237, 346, 369, 576, 818
Sau96I GGNCC 1 cut(s) 851
SchI GAGTC 2 cut(s) 69, 782
ScrFI CCNGG 3 cut(s) 645, 665, 849
SduI GDGCHC 2 cut(s) 412, 747
SfaNI GCATC 2 cut(s) 312, 445
SfcI CTRYAG 2 cut(s) 237, 537
SinI GGWCC 1 cut(s) 851
SmiMI CAYNNNNRTG 1 cut(s) 320
SmlI CTYRAG 1 cut(s) 455
SmoI CTYRAG 1 cut(s) 455
Sse9I AATT 3 cut(s) 94, 555, 654
SseBI AGGCCT 1 cut(s) 648
SsiI CCGC 5 cut(s) 46, 284, 460, 575, 607
SspMI CTAG 3 cut(s) 120, 332, 485
StuI AGGCCT 1 cut(s) 648
StyD4I CCNGG 3 cut(s) 643, 663, 847
TaaI ACNGT 2 cut(s) 9, 104
TaiI ACGT 3 cut(s) 225, 403, 798
TaqI TCGA 3 cut(s) 204, 339, 829
TasI AATT 3 cut(s) 94, 555, 654
TauI GCSGC 1 cut(s) 578
TfiI GAWTC 3 cut(s) 59, 280, 880
Tru1I TTAA 2 cut(s) 261, 875
Tru9I TTAA 2 cut(s) 261, 875
TscAI CASTG 4 cut(s) 12, 387, 429, 787
TseI GCWGC 4 cut(s) 236, 345, 368, 817
TspDTI ATGAA 5 cut(s) 43, 495, 580, 632, 777
TspGWI ACGGA 1 cut(s) 403
TspRI CASTG 4 cut(s) 12, 387, 429, 787
VpaK11BI GGWCC 1 cut(s) 851
XbaI TCTAGA 1 cut(s) 331
XmiI GTMKAC 2 cut(s) 106, 627
XspI CTAG 3 cut(s) 120, 332, 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.