Prupe.1G294800_v2.0.a1

rRNA N-glycosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0018098
Physical Location & Seq
Forward (+)
29188986 .. 29190628
1643 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G294800.1

Sequence Viewer

Length: 885 bp
ATGATACTATCCTTCTCCACTAAGAATGCAACCCCCGAGACATACCGCGACTTCATACAAGCTCTGCGAGACCAACTCACAGCCGGACGACCCACTTCAAACGGTATACCGGTGCTGCCTAGAAGAGAAGATGTGCCGGACGCTCAGCGCTTTCTTCTTGTTGATCTCACAAACTCTCAAGGAAATACCATCAGGCTCGCAATCGATGTGGTCAACGCTTACGTGGTGGGTTATGCTGCAGATGGTCGAGCCTATCTTCTTCAAGAAAATGCTCGTGATAATCGGCCTCCAATCCACACTTTATTCCGCGACGCCACCACTCGGATTGATCTGGGCTTCGACGGCAGCTACAGCGGGCTTTCGCGGGTTGCACGAGAAGCAGTAGAACGGAATACCCCACGTAATCGTGCTCGTAACCGAGCTGGGGCAAGTGCGCATGACAATACACCTGTGCTTGAGCAAATCCCCATGGGACGCAATGAACTAGATACCGCCATCAGCCTGTTGCGCTCCGCGTCATCCCCAACCAACCAAGCCTTAGGGTTTATCGTCATCATTCAGATGCTGTCAGAAGCGGCAAGGTTCCGGGCCATTGAGGGACTAGTCCGCACTACCATGCGGGAAACGTACGATCCCCTTATGCGAGGCCTTGCAATGGAGAGCCTGGAAACCCATTGGTCTGATCTCTCCGAACAGATCCAGAGGGCACAACAGCGCAATGAAACAGGCTTCGATAGGACTATTGTGCTCCATAATGTCGGAAATGAACGCCGCGAAGTAAACAGTGTTGATTCACCCTTCGTTCGGGGTGTCGCGATGCTGCTCTACGACCGAAACGGAAATTGCAACCCTGGATCTGGACCTCATCGTCATGATGAATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RIP

Protein Analysis

295

Amino Acids

32.87

Weight (kDa)

6.61

Isoelectric Point (pI)

31.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 867
Acc16I TGCGCA 1 cut(s) 435
AccI GTMKAC 1 cut(s) 106
AccII CGCG 6 cut(s) 48, 309, 364, 515, 774, 815
AclWI GGATC 3 cut(s) 626, 691, 862
AcyI GRCGYC 1 cut(s) 312
AfaI GTAC 1 cut(s) 629
AfeI AGCGCT 1 cut(s) 149
AfiI CCNNNNNNNGG 5 cut(s) 321, 424, 655, 804, 857
AgeI ACCGGT 1 cut(s) 109
AgsI TTSAA 2 cut(s) 99, 263
AhlI ACTAGT 1 cut(s) 601
AjnI CCWGG 2 cut(s) 663, 850
AluBI AGCT 3 cut(s) 62, 348, 422
AluI AGCT 3 cut(s) 62, 348, 422
Alw21I GWGCWC 2 cut(s) 412, 750
Alw26I GTCTC 2 cut(s) 32, 63
AlwI GGATC 3 cut(s) 626, 691, 862
AlwNI CAGNNNCTG 1 cut(s) 565
Ama87I CYCGRG 1 cut(s) 35
Aor51HI AGCGCT 1 cut(s) 149
AoxI GGCC 3 cut(s) 284, 588, 646
ApeKI GCWGC 4 cut(s) 115, 236, 345, 820
AsiGI ACCGGT 1 cut(s) 109
AspLEI GCGC 4 cut(s) 150, 436, 510, 717
AspS9I GGNCC 2 cut(s) 588, 860
AsuC2I CCSGG 1 cut(s) 587
AsuHPI GGTGA 1 cut(s) 786
AvaI CYCGRG 1 cut(s) 35
AvaII GGWCC 1 cut(s) 860
AxyI CCTNAGG 1 cut(s) 538
BaeGI GKGCMC 1 cut(s) 709
BauI CACGAG 2 cut(s) 273, 372
Bbv12I GWGCWC 2 cut(s) 412, 750
BbvI GCAGC 4 cut(s) 102, 223, 357, 807
BccI CCATC 3 cut(s) 197, 236, 503
BceAI ACGGC 1 cut(s) 358
BciT130I CCWGG 2 cut(s) 665, 852
BcnI CCSGG 1 cut(s) 587
BcoDI GTCTC 2 cut(s) 32, 63
BcuI ACTAGT 1 cut(s) 601
BfaI CTAG 3 cut(s) 120, 485, 602
BfmI CTRYAG 2 cut(s) 237, 349
BfoI RGCGCY 1 cut(s) 151
BisI GCNGC 6 cut(s) 116, 237, 346, 576, 772, 821
BlpI GCTNAGC 1 cut(s) 144
BlsI GCNGC 6 cut(s) 117, 238, 347, 577, 773, 822
Bme1390I CCNGG 3 cut(s) 587, 665, 852
Bme18I GGWCC 1 cut(s) 860
BmeT110I CYCGRG 1 cut(s) 35
BmgT120I GGNCC 2 cut(s) 588, 860
BmiI GGNNCC 1 cut(s) 584
BmrFI CCNGG 3 cut(s) 587, 665, 852
BmsI GCATC 2 cut(s) 552, 807
BplI GAGNNNNNCTC 2 cut(s) 60, 92
Bpu1102I GCTNAGC 1 cut(s) 144
BpuEI CTTGAG 2 cut(s) 162, 476
BpuMI CCSGG 1 cut(s) 587
Bsa29I ATCGAT 1 cut(s) 204
BsaAI YACGTR 2 cut(s) 223, 401
BsaHI GRCGYC 1 cut(s) 312
BsaI GGTCTC 1 cut(s) 63
BsaJI CCNNGG 2 cut(s) 468, 850
BsaWI WCCGGW 1 cut(s) 109
Bsc4I CCNNNNNNNGG 5 cut(s) 321, 424, 655, 804, 857
Bse118I RCCGGY 1 cut(s) 109
Bse21I CCTNAGG 1 cut(s) 538
Bse3DI GCAATG 3 cut(s) 484, 660, 724
BseBI CCWGG 2 cut(s) 665, 852
BseCI ATCGAT 1 cut(s) 204
BseDI CCNNGG 2 cut(s) 468, 850
BseGI GGATG 1 cut(s) 518
BseLI CCNNNNNNNGG 5 cut(s) 321, 424, 655, 804, 857
BseMI GCAATG 3 cut(s) 484, 660, 724
BseMII CTCAG 1 cut(s) 158
BseSI GKGCMC 1 cut(s) 709
BseXI GCAGC 4 cut(s) 102, 223, 357, 807
BseYI CCCAGC 1 cut(s) 422
Bsh1236I CGCG 6 cut(s) 48, 309, 364, 515, 774, 815
Bsh1285I CGRYCG 1 cut(s) 832
BshFI GGCC 3 cut(s) 286, 590, 648
BshTI ACCGGT 1 cut(s) 109
BshVI ATCGAT 1 cut(s) 204
BsiEI CGRYCG 1 cut(s) 832
BsiHKAI GWGCWC 2 cut(s) 412, 750
BsiHKCI CYCGRG 1 cut(s) 35
BsiSI CCGG 4 cut(s) 84, 110, 137, 586
BsiWI CGTACG 1 cut(s) 627
BslFI GGGAC 2 cut(s) 486, 612
BslI CCNNNNNNNGG 5 cut(s) 321, 424, 655, 804, 857
BsmAI GTCTC 2 cut(s) 32, 63
BsmFI GGGAC 2 cut(s) 486, 612
BsmI GAATGC 1 cut(s) 31
BsnI GGCC 3 cut(s) 286, 590, 648
Bso31I GGTCTC 1 cut(s) 63
BsoBI CYCGRG 1 cut(s) 35
Bsp1286I GDGCHC 3 cut(s) 412, 709, 750
Bsp143I GATC 6 cut(s) 163, 328, 631, 682, 696, 854
Bsp1720I GCTNAGC 1 cut(s) 144
Bsp19I CCATGG 1 cut(s) 468
Bsp68I TCGCGA 1 cut(s) 815
BspANI GGCC 3 cut(s) 286, 590, 648
BspCNI CTCAG 1 cut(s) 157
BspDI ATCGAT 1 cut(s) 204
BspFNI CGCG 6 cut(s) 48, 309, 364, 515, 774, 815
BspHI TCATGA 1 cut(s) 871
BspLI GGNNCC 1 cut(s) 584
BspMAI CTGCAG 1 cut(s) 241
BspPI GGATC 3 cut(s) 626, 691, 862
BspTNI GGTCTC 1 cut(s) 63
BsrDI GCAATG 3 cut(s) 484, 660, 724
BsrFI RCCGGY 1 cut(s) 109
BssAI RCCGGY 1 cut(s) 109
BssECI CCNNGG 2 cut(s) 468, 850
BssMI GATC 6 cut(s) 163, 328, 631, 682, 696, 854
BssNAI GTATAC 1 cut(s) 107
BssNI GRCGYC 1 cut(s) 312
BssSI CACGAG 2 cut(s) 273, 372
BssT1I CCWWGG 1 cut(s) 468
Bst1107I GTATAC 1 cut(s) 107
Bst2BI CACGAG 2 cut(s) 273, 372
Bst2UI CCWGG 2 cut(s) 665, 852
Bst4CI ACNGT 2 cut(s) 104, 785
Bst6I CTCTTC 1 cut(s) 118
BstACI GRCGYC 1 cut(s) 312
BstBAI YACGTR 2 cut(s) 223, 401
BstC8I GCNNGC 2 cut(s) 198, 356
BstDEI CTNAG 3 cut(s) 21, 144, 538
BstDSI CCRYGG 1 cut(s) 468
BstF5I GGATG 1 cut(s) 518
BstFNI CGCG 6 cut(s) 48, 309, 364, 515, 774, 815
BstH2I RGCGCY 1 cut(s) 151
BstHHI GCGC 4 cut(s) 150, 436, 510, 717
BstKTI GATC 6 cut(s) 166, 331, 634, 685, 699, 857
BstMAI GTCTC 2 cut(s) 32, 63
BstMBI GATC 6 cut(s) 163, 328, 631, 682, 696, 854
BstMCI CGRYCG 1 cut(s) 832
BstMWI GCNNNNNNNGC 4 cut(s) 342, 351, 377, 507
BstNI CCWGG 2 cut(s) 665, 852
BstSCI CCNGG 3 cut(s) 585, 663, 850
BstSFI CTRYAG 2 cut(s) 237, 349
BstSLI GKGCMC 1 cut(s) 709
BstUI CGCG 6 cut(s) 48, 309, 364, 515, 774, 815
BstV1I GCAGC 4 cut(s) 102, 223, 357, 807
BstX2I RGATCY 2 cut(s) 696, 854
BstYI RGATCY 2 cut(s) 696, 854
BstZ17I GTATAC 1 cut(s) 107
Bsu15I ATCGAT 1 cut(s) 204
Bsu36I CCTNAGG 1 cut(s) 538
BsuRI GGCC 3 cut(s) 286, 590, 648
BsuTUI ATCGAT 1 cut(s) 204
BtgI CCRYGG 1 cut(s) 468
BtgZI GCGATG 1 cut(s) 830
BtsCI GGATG 1 cut(s) 518
BtsIMutI CAGTG 1 cut(s) 790
BtuMI TCGCGA 1 cut(s) 815
Cac8I GCNNGC 2 cut(s) 198, 356
CaiI CAGNNNCTG 1 cut(s) 565
CciI TCATGA 1 cut(s) 871
CfoI GCGC 4 cut(s) 150, 436, 510, 717
Cfr10I RCCGGY 1 cut(s) 109
Cfr13I GGNCC 2 cut(s) 588, 860
ClaI ATCGAT 1 cut(s) 204
CseI GACGC 4 cut(s) 149, 320, 483, 504
Csp6I GTAC 1 cut(s) 628
CspAI ACCGGT 1 cut(s) 109
CspCI CAANNNNNGTGG 4 cut(s) 189, 224, 307, 342
CviAII CATG 4 cut(s) 437, 469, 616, 872
CviQI GTAC 1 cut(s) 628
DdeI CTNAG 3 cut(s) 21, 144, 538
DpnI GATC 6 cut(s) 165, 330, 633, 684, 698, 856
DpnII GATC 6 cut(s) 163, 328, 631, 682, 696, 854
DrdI GACNNNNNNGTC 1 cut(s) 867
DseDI GACNNNNNNGTC 1 cut(s) 867
Eam1104I CTCTTC 1 cut(s) 118
EarI CTCTTC 1 cut(s) 118
Eco130I CCWWGG 1 cut(s) 468
Eco147I AGGCCT 1 cut(s) 648
Eco31I GGTCTC 1 cut(s) 63
Eco47I GGWCC 1 cut(s) 860
Eco47III AGCGCT 1 cut(s) 149
Eco81I CCTNAGG 1 cut(s) 538
Eco88I CYCGRG 1 cut(s) 35
EcoRII CCWGG 2 cut(s) 663, 850
EcoT14I CCWWGG 1 cut(s) 468
ErhI CCWWGG 1 cut(s) 468
FaeI CATG 4 cut(s) 440, 472, 619, 875
FaqI GGGAC 2 cut(s) 486, 612
FatI CATG 4 cut(s) 436, 468, 615, 871
FauI CCCGC 3 cut(s) 347, 357, 612
FblI GTMKAC 1 cut(s) 106
Fnu4HI GCNGC 6 cut(s) 116, 237, 346, 576, 772, 821
FokI GGATG 1 cut(s) 505
Fsp4HI GCNGC 6 cut(s) 116, 237, 346, 576, 772, 821
FspAI RTGCGCAY 1 cut(s) 435
FspBI CTAG 3 cut(s) 120, 485, 602
FspI TGCGCA 1 cut(s) 435
GlaI GCGC 4 cut(s) 149, 435, 509, 716
GluI GCNGC 6 cut(s) 116, 237, 346, 576, 772, 821
GsaI CCCAGC 1 cut(s) 426
HaeII RGCGCY 1 cut(s) 151
HaeIII GGCC 3 cut(s) 286, 590, 648
HapII CCGG 4 cut(s) 84, 110, 137, 586
HgaI GACGC 4 cut(s) 149, 320, 483, 504
HhaI GCGC 4 cut(s) 150, 436, 510, 717
Hin1I GRCGYC 1 cut(s) 312
Hin1II CATG 4 cut(s) 440, 472, 619, 875
Hin6I GCGC 4 cut(s) 148, 434, 508, 715
HinP1I GCGC 4 cut(s) 148, 434, 508, 715
HincII GTYRAC 1 cut(s) 214
HindII GTYRAC 1 cut(s) 214
HinfI GANTC 1 cut(s) 791
HpaII CCGG 4 cut(s) 84, 110, 137, 586
HphI GGTGA 1 cut(s) 786
Hpy166II GTNNAC 3 cut(s) 107, 214, 781
Hpy188I TCNGA 6 cut(s) 324, 561, 571, 682, 691, 761
Hpy188III TCNNGA 6 cut(s) 263, 275, 700, 814, 858, 872
Hpy8I GTNNAC 3 cut(s) 107, 214, 781
Hpy99I CGWCG 2 cut(s) 314, 344
HpyAV CCTTC 2 cut(s) 22, 808
HpyCH4III ACNGT 2 cut(s) 104, 785
HpyCH4IV ACGT 3 cut(s) 222, 400, 626
HpyCH4V TGCA 5 cut(s) 29, 239, 371, 653, 846
HpyF10VI GCNNNNNNNGC 4 cut(s) 342, 351, 377, 507
HpyF3I CTNAG 3 cut(s) 21, 144, 538
HpySE526I ACGT 3 cut(s) 222, 400, 626
Hsp92I GRCGYC 1 cut(s) 312
Hsp92II CATG 4 cut(s) 440, 472, 619, 875
HspAI GCGC 4 cut(s) 148, 434, 508, 715
Kzo9I GATC 6 cut(s) 163, 328, 631, 682, 696, 854
LmnI GCTCC 2 cut(s) 515, 753
Lsp1109I GCAGC 4 cut(s) 102, 223, 357, 807
LweI GCATC 2 cut(s) 552, 807
MaeI CTAG 3 cut(s) 120, 485, 602
MaeII ACGT 3 cut(s) 222, 400, 626
MaeIII GTNAC 1 cut(s) 413
MalI GATC 6 cut(s) 165, 330, 633, 684, 698, 856
MboI GATC 6 cut(s) 163, 328, 631, 682, 696, 854
MboII GAAGA 5 cut(s) 135, 140, 146, 248, 251
MflI RGATCY 2 cut(s) 696, 854
MhlI GDGCHC 3 cut(s) 412, 709, 750
MluCI AATT 2 cut(s) 841, 878
MmeI TCCRAC 1 cut(s) 739
MnlI CCTC 5 cut(s) 297, 589, 638, 696, 873
MslI CAYNNNNRTG 3 cut(s) 560, 614, 870
MspA1I CMGCKG 1 cut(s) 354
MspI CCGG 4 cut(s) 84, 110, 137, 586
MspR9I CCNGG 3 cut(s) 587, 665, 852
Mva1269I GAATGC 1 cut(s) 31
MvaI CCWGG 2 cut(s) 665, 852
MvnI CGCG 6 cut(s) 48, 309, 364, 515, 774, 815
MwoI GCNNNNNNNGC 4 cut(s) 342, 351, 377, 507
NciI CCSGG 1 cut(s) 587
NcoI CCATGG 1 cut(s) 468
NdeII GATC 6 cut(s) 163, 328, 631, 682, 696, 854
NlaIII CATG 4 cut(s) 440, 472, 619, 875
NlaIV GGNNCC 1 cut(s) 584
NruI TCGCGA 1 cut(s) 815
NsbI TGCGCA 1 cut(s) 435
PagI TCATGA 1 cut(s) 871
PceI AGGCCT 1 cut(s) 648
PctI GAATGC 1 cut(s) 31
PfeI GAWTC 1 cut(s) 791
Pfl23II CGTACG 1 cut(s) 627
PinAI ACCGGT 1 cut(s) 109
PkrI GCNGC 6 cut(s) 117, 238, 347, 577, 773, 822
Ppu21I YACGTR 2 cut(s) 223, 401
Psp6I CCWGG 2 cut(s) 663, 850
PspFI CCCAGC 1 cut(s) 422
PspGI CCWGG 2 cut(s) 663, 850
PspLI CGTACG 1 cut(s) 627
PspN4I GGNNCC 1 cut(s) 584
PspPI GGNCC 2 cut(s) 588, 860
PstI CTGCAG 1 cut(s) 241
PstNI CAGNNNCTG 1 cut(s) 565
PsuI RGATCY 2 cut(s) 696, 854
RruI TCGCGA 1 cut(s) 815
RsaI GTAC 1 cut(s) 629
RsaNI GTAC 1 cut(s) 628
RseI CAYNNNNRTG 3 cut(s) 560, 614, 870
SatI GCNGC 6 cut(s) 116, 237, 346, 576, 772, 821
Sau3AI GATC 6 cut(s) 163, 328, 631, 682, 696, 854
Sau96I GGNCC 2 cut(s) 588, 860
ScrFI CCNGG 3 cut(s) 587, 665, 852
SduI GDGCHC 3 cut(s) 412, 709, 750
SetI ASST 9 cut(s) 64, 225, 350, 403, 424, 451, 584, 629, 865
SfaNI GCATC 2 cut(s) 552, 807
SfcI CTRYAG 2 cut(s) 237, 349
SinI GGWCC 1 cut(s) 860
SmiMI CAYNNNNRTG 3 cut(s) 560, 614, 870
SmlI CTYRAG 2 cut(s) 177, 455
SmoI CTYRAG 2 cut(s) 177, 455
SpeI ACTAGT 1 cut(s) 601
Sse9I AATT 2 cut(s) 841, 878
SseBI AGGCCT 1 cut(s) 648
SspMI CTAG 3 cut(s) 120, 485, 602
StuI AGGCCT 1 cut(s) 648
StyD4I CCNGG 3 cut(s) 585, 663, 850
StyI CCWWGG 1 cut(s) 468
TaaI ACNGT 2 cut(s) 104, 785
TaiI ACGT 3 cut(s) 225, 403, 629
TaqI TCGA 4 cut(s) 204, 247, 339, 732
TaqII GACCGA 1 cut(s) 846
TasI AATT 2 cut(s) 841, 878
TauI GCSGC 2 cut(s) 578, 774
TfiI GAWTC 1 cut(s) 791
TscAI CASTG 1 cut(s) 790
TseI GCWGC 4 cut(s) 115, 236, 345, 820
TspDTI ATGAA 4 cut(s) 43, 495, 735, 780
TspGWI ACGGA 2 cut(s) 403, 852
TspRI CASTG 1 cut(s) 790
VpaK11BI GGWCC 1 cut(s) 860
XmiI GTMKAC 1 cut(s) 106
XspI CTAG 3 cut(s) 120, 485, 602
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.