MD06G1085500.v1.1

rRNA N-glycosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0018098
Physical Location & Seq
Reverse (-)
20981546 .. 20982451
906 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1085500.v1.1.491

Sequence Viewer

Length: 906 bp
ATGGCACTATCCTTCTCCATTAAGAATGCGACCACCACGACATACAGAACTTTCATAGAAGCTCTGCGAGCCCAACTAACAGCCGGAGGATCCACATCACATGGGATACCGGTGCTGCGTCGGAGACAAGATGTGAAGGATGACCAGCGCTTTGTTCTTGTTAATCTCACCAACTACGACAGTTATACAATAACTGTCGCAATCGATGTGGTGAACGCTTACGTTGTGGGTTATTGTGCCGGTACTCGTTCCTACTTTCTTCGAGATCCTGCAACTCATCCGCCTCCACTCCATAGATTATTCCCCGGCACCACGCGGACTACACTGCCCTTCGCCGGGGACTACCTCGGGCTTGGCCGAGCTGCCCAGGAGGCACTACAACAGAATACAAACCGTAACCGAGCAGCAGGGTCACGTATCCATGAGAACATATCTATGCGTGAGCGGATCCCGCTGGGGCCCGGAGAACTAGATAACGCAATAAGCCAGTTGCGCTACGCAGAATCTGCATCCTCCCAAGCCGCCGCGTTTATTGTGATTATTCAAATAGTCTCTGAAGCCGCAAGGTTCCGGTACATTCAGGGACAAGTCCGCGACCGCATTCGTGACGGAACTTCCGCTGAGCCGGACCCTGCAATGCTCAGCCTCGAAAACAGTTGGTCTAATCTGTCCGAGCAGATCCAGATGGTACCGGCCAATCAATTACTCTTCATCAATAATGGGTCTGTTCAGATCCGAAAAGCCGACAATAGTATTGTCCTAGTAAAGAGTGTTGACTCAGACGCCGTTCGCGGTGTCGCGTTCCTGCTTTACTGCGGAGGCAACCCTCCTGCACCGAACAGTGAGAGTGCCCGGACGTCCAAGGTTACTGTTCAGAAGCCAACCCTCGCCAAGAAGAAGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RIP

Protein Analysis

302

Amino Acids

33.01

Weight (kDa)

9.75

Isoelectric Point (pI)

34.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RIP PF00161 11 - 228 1.9e-44 Ribosome inactivating protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 860
Acc65I GGTACC 1 cut(s) 688
AccB1I GGYRCC 2 cut(s) 308, 688
AccBSI CCGCTC 1 cut(s) 445
AccII CGCG 5 cut(s) 316, 527, 594, 792, 800
AclWI GGATC 7 cut(s) 84, 97, 260, 442, 455, 673, 727
AcoI YGGCCR 2 cut(s) 355, 693
AcuI CTGAAG 1 cut(s) 576
AcyI GRCGYC 2 cut(s) 783, 857
AfaI GTAC 3 cut(s) 244, 575, 690
AfeI AGCGCT 1 cut(s) 149
AfiI CCNNNNNNNGG 2 cut(s) 335, 336
AgeI ACCGGT 1 cut(s) 109
AgsI TTSAA 1 cut(s) 545
AjnI CCWGG 1 cut(s) 366
AluBI AGCT 2 cut(s) 62, 362
AluI AGCT 2 cut(s) 62, 362
Alw26I GTCTC 2 cut(s) 118, 556
AlwI GGATC 7 cut(s) 84, 97, 260, 442, 455, 673, 727
AlwNI CAGNNNCTG 1 cut(s) 506
Ama87I CYCGRG 1 cut(s) 347
Aor51HI AGCGCT 1 cut(s) 149
AoxI GGCC 3 cut(s) 355, 458, 693
ApaI GGGCCC 1 cut(s) 462
ApeKI GCWGC 3 cut(s) 115, 362, 404
ArsI GACNNNNNNTTYG 2 cut(s) 134, 166
AsiGI ACCGGT 1 cut(s) 109
Asp718I GGTACC 1 cut(s) 688
AspLEI GCGC 2 cut(s) 150, 495
AspS9I GGNCC 3 cut(s) 458, 459, 628
AsuC2I CCSGG 4 cut(s) 306, 337, 462, 853
AsuHPI GGTGA 2 cut(s) 160, 223
AvaI CYCGRG 1 cut(s) 347
AvaII GGWCC 1 cut(s) 628
BaeGI GKGCMC 2 cut(s) 462, 853
BamHI GGATCC 2 cut(s) 89, 447
BanI GGYRCC 2 cut(s) 308, 688
BanII GRGCYC 2 cut(s) 73, 462
BbvI GCAGC 3 cut(s) 102, 349, 416
BccI CCATC 1 cut(s) 679
BceAI ACGGC 1 cut(s) 770
BciT130I CCWGG 1 cut(s) 368
BciVI GTATCC 2 cut(s) 99, 428
BcnI CCSGG 4 cut(s) 306, 337, 462, 853
BcoDI GTCTC 2 cut(s) 118, 556
BfaI CTAG 2 cut(s) 470, 761
BfoI RGCGCY 1 cut(s) 151
BfuI GTATCC 2 cut(s) 99, 428
BglI GCCNNNNNGGC 1 cut(s) 371
BisI GCNGC 6 cut(s) 116, 363, 405, 522, 525, 561
BlpI GCTNAGC 2 cut(s) 621, 641
BlsI GCNGC 6 cut(s) 117, 364, 406, 523, 526, 562
Bme1390I CCNGG 5 cut(s) 306, 337, 368, 462, 853
Bme18I GGWCC 1 cut(s) 628
BmeT110I CYCGRG 1 cut(s) 347
BmgT120I GGNCC 3 cut(s) 458, 459, 628
BmiI GGNNCC 8 cut(s) 91, 310, 449, 459, 460, 569, 630, 690
BmrFI CCNGG 5 cut(s) 306, 337, 368, 462, 853
BmsI GCATC 1 cut(s) 518
Bpu1102I GCTNAGC 2 cut(s) 621, 641
BpuMI CCSGG 4 cut(s) 306, 337, 462, 853
Bsa29I ATCGAT 1 cut(s) 204
BsaAI YACGTR 1 cut(s) 416
BsaBI GATNNNNATC 1 cut(s) 94
BsaHI GRCGYC 2 cut(s) 783, 857
BsaJI CCNNGG 5 cut(s) 304, 336, 346, 366, 861
BsaWI WCCGGW 2 cut(s) 109, 570
Bsc4I CCNNNNNNNGG 2 cut(s) 335, 336
Bse118I RCCGGY 3 cut(s) 109, 239, 691
Bse1I ACTGG 1 cut(s) 487
Bse3DI GCAATG 1 cut(s) 642
Bse8I GATNNNNATC 1 cut(s) 94
BseBI CCWGG 1 cut(s) 368
BseCI ATCGAT 1 cut(s) 204
BseDI CCNNGG 5 cut(s) 304, 336, 346, 366, 861
BseGI GGATG 3 cut(s) 145, 277, 509
BseJI GATNNNNATC 1 cut(s) 94
BseLI CCNNNNNNNGG 2 cut(s) 335, 336
BseMI GCAATG 1 cut(s) 642
BseMII CTCAG 3 cut(s) 612, 655, 792
BseNI ACTGG 1 cut(s) 487
BseSI GKGCMC 2 cut(s) 462, 853
BseXI GCAGC 3 cut(s) 102, 349, 416
BseYI CCCAGC 1 cut(s) 454
BsgI GTGCAG 1 cut(s) 816
Bsh1236I CGCG 5 cut(s) 316, 527, 594, 792, 800
Bsh1285I CGRYCG 1 cut(s) 598
BshFI GGCC 3 cut(s) 357, 460, 695
BshNI GGYRCC 2 cut(s) 308, 688
BshTI ACCGGT 1 cut(s) 109
BshVI ATCGAT 1 cut(s) 204
BsiEI CGRYCG 1 cut(s) 598
BsiHKCI CYCGRG 1 cut(s) 347
BslFI GGGAC 2 cut(s) 353, 597
BslI CCNNNNNNNGG 2 cut(s) 335, 336
BsmAI GTCTC 2 cut(s) 118, 556
BsmFI GGGAC 2 cut(s) 353, 597
BsmI GAATGC 2 cut(s) 31, 600
BsnI GGCC 3 cut(s) 357, 460, 695
BsoBI CYCGRG 1 cut(s) 347
Bsp120I GGGCCC 1 cut(s) 458
Bsp1286I GDGCHC 3 cut(s) 73, 462, 853
Bsp143I GATC 5 cut(s) 89, 265, 447, 678, 732
Bsp1720I GCTNAGC 2 cut(s) 621, 641
BspANI GGCC 3 cut(s) 357, 460, 695
BspCNI CTCAG 3 cut(s) 613, 654, 791
BspDI ATCGAT 1 cut(s) 204
BspFNI CGCG 5 cut(s) 316, 527, 594, 792, 800
BspLI GGNNCC 8 cut(s) 91, 310, 449, 459, 460, 569, 630, 690
BspPI GGATC 7 cut(s) 84, 97, 260, 442, 455, 673, 727
BspT107I GGYRCC 2 cut(s) 308, 688
BsrBI CCGCTC 1 cut(s) 445
BsrDI GCAATG 1 cut(s) 642
BsrFI RCCGGY 3 cut(s) 109, 239, 691
BsrI ACTGG 1 cut(s) 487
BssAI RCCGGY 3 cut(s) 109, 239, 691
BssECI CCNNGG 5 cut(s) 304, 336, 346, 366, 861
BssMI GATC 5 cut(s) 89, 265, 447, 678, 732
BssNI GRCGYC 2 cut(s) 783, 857
BssT1I CCWWGG 1 cut(s) 861
Bst2UI CCWGG 1 cut(s) 368
Bst4CI ACNGT 6 cut(s) 182, 196, 395, 656, 842, 871
Bst6I CTCTTC 1 cut(s) 713
BstACI GRCGYC 2 cut(s) 783, 857
BstAPI GCANNNNNTGC 1 cut(s) 506
BstBAI YACGTR 1 cut(s) 416
BstC8I GCNNGC 1 cut(s) 69
BstDEI CTNAG 3 cut(s) 621, 641, 778
BstF5I GGATG 3 cut(s) 145, 277, 509
BstFNI CGCG 5 cut(s) 316, 527, 594, 792, 800
BstH2I RGCGCY 1 cut(s) 151
BstHHI GCGC 2 cut(s) 150, 495
BstKTI GATC 5 cut(s) 92, 268, 450, 681, 735
BstMAI GTCTC 2 cut(s) 118, 556
BstMBI GATC 5 cut(s) 89, 265, 447, 678, 732
BstMCI CGRYCG 1 cut(s) 598
BstMWI GCNNNNNNNGC 5 cut(s) 68, 371, 451, 492, 506
BstNI CCWGG 1 cut(s) 368
BstSCI CCNGG 5 cut(s) 304, 335, 366, 460, 851
BstSLI GKGCMC 2 cut(s) 462, 853
BstUI CGCG 5 cut(s) 316, 527, 594, 792, 800
BstV1I GCAGC 3 cut(s) 102, 349, 416
BstX2I RGATCY 5 cut(s) 89, 265, 447, 678, 732
BstYI RGATCY 5 cut(s) 89, 265, 447, 678, 732
Bsu15I ATCGAT 1 cut(s) 204
BsuI GTATCC 2 cut(s) 99, 428
BsuRI GGCC 3 cut(s) 357, 460, 695
BsuTUI ATCGAT 1 cut(s) 204
BtsCI GGATG 3 cut(s) 145, 277, 509
BtsI GCAGTG 1 cut(s) 323
BtsIMutI CAGTG 2 cut(s) 323, 847
Cac8I GCNNGC 1 cut(s) 69
CaiI CAGNNNCTG 1 cut(s) 506
CfoI GCGC 2 cut(s) 150, 495
Cfr10I RCCGGY 3 cut(s) 109, 239, 691
Cfr13I GGNCC 3 cut(s) 458, 459, 628
ClaI ATCGAT 1 cut(s) 204
CseI GACGC 2 cut(s) 107, 791
Csp6I GTAC 3 cut(s) 243, 574, 689
CspAI ACCGGT 1 cut(s) 109
CspCI CAANNNNNGTGG 2 cut(s) 189, 224
CviAII CATG 2 cut(s) 101, 422
CviQI GTAC 3 cut(s) 243, 574, 689
DdeI CTNAG 3 cut(s) 621, 641, 778
DpnI GATC 5 cut(s) 91, 267, 449, 680, 734
DpnII GATC 5 cut(s) 89, 265, 447, 678, 732
EaeI YGGCCR 2 cut(s) 355, 693
Eam1104I CTCTTC 1 cut(s) 713
EarI CTCTTC 1 cut(s) 713
EciI GGCGGA 1 cut(s) 270
Eco130I CCWWGG 1 cut(s) 861
Eco24I GRGCYC 2 cut(s) 73, 462
Eco47I GGWCC 1 cut(s) 628
Eco47III AGCGCT 1 cut(s) 149
Eco57I CTGAAG 1 cut(s) 576
Eco88I CYCGRG 1 cut(s) 347
EcoO109I RGGNCCY 1 cut(s) 458
EcoRII CCWGG 1 cut(s) 366
EcoT14I CCWWGG 1 cut(s) 861
EcoT38I GRGCYC 2 cut(s) 73, 462
ErhI CCWWGG 1 cut(s) 861
FaeI CATG 2 cut(s) 104, 425
FaiI YATR 8 cut(s) 43, 56, 102, 186, 294, 423, 431, 437
FaqI GGGAC 2 cut(s) 353, 597
FatI CATG 2 cut(s) 100, 421
FauI CCCGC 1 cut(s) 459
Fnu4HI GCNGC 6 cut(s) 116, 363, 405, 522, 525, 561
FokI GGATG 3 cut(s) 152, 264, 496
FriOI GRGCYC 2 cut(s) 73, 462
Fsp4HI GCNGC 6 cut(s) 116, 363, 405, 522, 525, 561
FspBI CTAG 2 cut(s) 470, 761
GlaI GCGC 2 cut(s) 149, 494
GluI GCNGC 6 cut(s) 116, 363, 405, 522, 525, 561
GsaI CCCAGC 1 cut(s) 458
HaeII RGCGCY 1 cut(s) 151
HaeIII GGCC 3 cut(s) 357, 460, 695
HgaI GACGC 2 cut(s) 107, 791
HhaI GCGC 2 cut(s) 150, 495
Hin1I GRCGYC 2 cut(s) 783, 857
Hin1II CATG 2 cut(s) 104, 425
Hin6I GCGC 2 cut(s) 148, 493
HinP1I GCGC 2 cut(s) 148, 493
HincII GTYRAC 1 cut(s) 775
HindII GTYRAC 1 cut(s) 775
HinfI GANTC 2 cut(s) 503, 776
HphI GGTGA 2 cut(s) 160, 223
Hpy166II GTNNAC 2 cut(s) 214, 775
Hpy188I TCNGA 7 cut(s) 123, 556, 673, 732, 737, 781, 876
Hpy188III TCNNGA 3 cut(s) 263, 605, 682
Hpy8I GTNNAC 2 cut(s) 214, 775
Hpy99I CGWCG 1 cut(s) 123
HpyAV CCTTC 3 cut(s) 22, 130, 340
HpyCH4III ACNGT 6 cut(s) 182, 196, 395, 656, 842, 871
HpyCH4IV ACGT 3 cut(s) 222, 415, 857
HpyCH4V TGCA 4 cut(s) 272, 509, 635, 833
HpyF10VI GCNNNNNNNGC 5 cut(s) 68, 371, 451, 492, 506
HpyF3I CTNAG 3 cut(s) 621, 641, 778
HpySE526I ACGT 3 cut(s) 222, 415, 857
Hsp92I GRCGYC 2 cut(s) 783, 857
Hsp92II CATG 2 cut(s) 104, 425
HspAI GCGC 2 cut(s) 148, 493
KpnI GGTACC 1 cut(s) 692
Kzo9I GATC 5 cut(s) 89, 265, 447, 678, 732
Lsp1109I GCAGC 3 cut(s) 102, 349, 416
LweI GCATC 1 cut(s) 518
MaeI CTAG 2 cut(s) 470, 761
MaeII ACGT 3 cut(s) 222, 415, 857
MaeIII GTNAC 4 cut(s) 395, 411, 605, 865
MalI GATC 5 cut(s) 91, 267, 449, 680, 734
MbiI CCGCTC 1 cut(s) 445
MboI GATC 5 cut(s) 89, 265, 447, 678, 732
MboII GAAGA 2 cut(s) 251, 700
MflI RGATCY 5 cut(s) 89, 265, 447, 678, 732
MhlI GDGCHC 3 cut(s) 73, 462, 853
MluCI AATT 1 cut(s) 701
MlyI GAGTC 1 cut(s) 770
MmeI TCCRAC 1 cut(s) 101
MnlI CCTC 9 cut(s) 80, 294, 356, 364, 523, 656, 812, 837, 896
MseI TTAA 2 cut(s) 21, 162
MslI CAYNNNNRTG 1 cut(s) 434
MspA1I CMGCKG 2 cut(s) 454, 620
MspR9I CCNGG 5 cut(s) 306, 337, 368, 462, 853
Mva1269I GAATGC 2 cut(s) 31, 600
MvaI CCWGG 1 cut(s) 368
MvnI CGCG 5 cut(s) 316, 527, 594, 792, 800
MwoI GCNNNNNNNGC 5 cut(s) 68, 371, 451, 492, 506
NciI CCSGG 4 cut(s) 306, 337, 462, 853
NdeII GATC 5 cut(s) 89, 265, 447, 678, 732
NlaIII CATG 2 cut(s) 104, 425
NlaIV GGNNCC 8 cut(s) 91, 310, 449, 459, 460, 569, 630, 690
NmeAIII GCCGAG 1 cut(s) 383
NmuCI GTSAC 2 cut(s) 411, 605
PctI GAATGC 2 cut(s) 31, 600
PfeI GAWTC 1 cut(s) 503
PinAI ACCGGT 1 cut(s) 109
PkrI GCNGC 6 cut(s) 117, 364, 406, 523, 526, 562
PleI GAGTC 1 cut(s) 770
PpsI GAGTC 1 cut(s) 770
Ppu21I YACGTR 1 cut(s) 416
Psp6I CCWGG 1 cut(s) 366
PspFI CCCAGC 1 cut(s) 454
PspGI CCWGG 1 cut(s) 366
PspN4I GGNNCC 8 cut(s) 91, 310, 449, 459, 460, 569, 630, 690
PspOMI GGGCCC 1 cut(s) 458
PspPI GGNCC 3 cut(s) 458, 459, 628
PstNI CAGNNNCTG 1 cut(s) 506
PsuI RGATCY 5 cut(s) 89, 265, 447, 678, 732
RsaI GTAC 3 cut(s) 244, 575, 690
RsaNI GTAC 3 cut(s) 243, 574, 689
RseI CAYNNNNRTG 1 cut(s) 434
SaqAI TTAA 2 cut(s) 21, 162
SatI GCNGC 6 cut(s) 116, 363, 405, 522, 525, 561
Sau3AI GATC 5 cut(s) 89, 265, 447, 678, 732
Sau96I GGNCC 3 cut(s) 458, 459, 628
SchI GAGTC 1 cut(s) 770
ScrFI CCNGG 5 cut(s) 306, 337, 368, 462, 853
SduI GDGCHC 3 cut(s) 73, 462, 853
SetI ASST 8 cut(s) 64, 225, 348, 364, 418, 569, 860, 867
SfaNI GCATC 1 cut(s) 518
SinI GGWCC 1 cut(s) 628
SmiMI CAYNNNNRTG 1 cut(s) 434
Sse9I AATT 1 cut(s) 701
SspMI CTAG 2 cut(s) 470, 761
StyD4I CCNGG 5 cut(s) 304, 335, 366, 460, 851
StyI CCWWGG 1 cut(s) 861
TaaI ACNGT 6 cut(s) 182, 196, 395, 656, 842, 871
TaiI ACGT 3 cut(s) 225, 418, 860
TaqI TCGA 3 cut(s) 204, 262, 648
TasI AATT 1 cut(s) 701
TauI GCSGC 3 cut(s) 524, 527, 563
TfiI GAWTC 1 cut(s) 503
Tru1I TTAA 2 cut(s) 21, 162
Tru9I TTAA 2 cut(s) 21, 162
TscAI CASTG 2 cut(s) 330, 847
TseFI GTSAC 2 cut(s) 411, 605
TseI GCWGC 3 cut(s) 115, 362, 404
Tsp45I GTSAC 2 cut(s) 411, 605
TspDTI ATGAA 2 cut(s) 43, 700
TspGWI ACGGA 1 cut(s) 624
TspRI CASTG 2 cut(s) 330, 847
VpaK11BI GGWCC 1 cut(s) 628
XspI CTAG 2 cut(s) 470, 761
ZraI GACGTC 1 cut(s) 858
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.