Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0012154

Physical Location & Seq

Genomic Coordinates Reverse (-)

14705252 .. 14708116

Gene Structure 2865 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1073300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2172 bp

ATGAGATGGCTTTCTTCTTTAGCTTCTAGGGCACATGCTGCTGTTGCACTCCGAATCCTCCCCCTCCCCGACCAAGCCCCCTACAGTCTCAGTCAAATTCAGACAGAAAATGCTTCATTTGAAAACAAACCCAGAATTAGAAAAGCTTCTGCTCCTCGCAACATTCGATTTGGCGCTTCCCGTCCTCCCAAACCTAAAGGTAATTCTTCTTGTACGCCCAATCCTAATCCTAATTTGGTTTCCATTTCAAACATTCCCATTTCAGCTGGTAATGCCCATCACAACCATAAACCAATTGATCACTCATACATTGTCCAAATTCTTTCACGGAAGGACTGGTTTTTATTGCTCGCCCACGAGTTGAAGGCCCAGAGGATCGTTTTGAATCCTCAGTTTGTTGCTAGTGTCTTGCAAAACCAAGAAAACCCATCACTCTCTTTGAAGTTTTACTTATGGGTTTCGAGTAACGACCCCTTGTTGTCAAAGAATCAATCCGTTCGCGGTATCTTAGCCAACACCCTTTACCGGAGAGGCCCCGTTGTGTTGTCTGTTGAATTGCTAAATGATATTAAGAATTCTGGTTTAATGGTCCCTGAAGACTTGCTTTGCATATTGATCGGCAGTTGGGGGAGGTTGGGTTTGTCAAAATATTGTGCTGAGGTTTCTGGGCAAATTTCATTTTTGGGTCTCCGTCTTAGCACAAGGTTGTATAATGCCGTGATCAATGCGTTGGTCAAGTCCAATTCACTTGACTTGGCTTATTTGAAATTCCAACAGATGCCAGCAGATAACTGTGGTCCCGATAGATTCACTTACAATATCCTCATTTATGGAGTCTGCAAGATTGGTATTGTGGATGAGGCACTTCGCTTAGTTAAACAAATGGAGGGCTTGGGATATTTTCCCAATGTTTGGACGTATACAATCCTCATTGATGGGTTTTGTAATACAAAGAGGGTTGGCGAAGCCTTCTGGGTTTTGGAGATAATGAGGGAGACGAATGTGACTCCTAATGAAGCTACTATCAGATCATTAGTTCATGGTGTGTTTCGTTCCACGGCCCCCAGTGAAGCTTTTGAGTTGTTATTGAGTTTTGTTGAAAGGGAGTCTGTATTGTTCAAGGTCGCCTGTGATACCGTACTGTATTGCCTTTCGAATCGTTGTATGGCAAAAGAGATTGTTTCGTTTTTGAAGAAGAGTGATGCAAAAGGTTATTTGCCTGAGAGTTCAACATTCAACATCATAATGGTTTGTTTGATAAAGGAATTAGCTCCTTTTCGGAATCAGAACGAGGTTCATGATATATTTCAAAGTTTTATACGGCGTGGTGTGAAACCGGGATTTAGCACTTATCTTGCATTGATTGAAGCCATGTACAAGGCAGGAAAAGCTGGTCACGGGAATCAAATCTTTGATCAAATGATCAAAGAGGGACTTGTATCAGATGTCTTCTCATATAACATGGTAATTGATTGCTTATGCAAAGCCCAAATGTTGGACAGGGCATCAAAGGTTTTTGAAGATATGCAGTGCAAAGGTATCCCTCCTAACCTTGTTACTTTCAATACACTTCTTACCGGATATAGCAAGGCTGGAGAAGTAGGTAAGGCACATGAACTATTGGCAATGCTCTTGGAACAGGGGATTAAACCCGATAAGTTCACTTTTAGTTCACTAATTGATGGCCTTTGTCGGGCGAACCGGATAGATGATGCTTTTGACTGTTTTGCTGAGATGGTTAGGTGGAGGGTCACTCCAAATTCCATCACATACAACATACTGATACGCTCTCTGTGTTTCGTTGGAGACATTTCTAGAGCGGTGAGAGCAATGAAGAAAATGCAGGCGAATGGAATAAAACCAGATGCTTACTCGTTTAATGCTCTTATTCAAGGTCTTTGTAGGATGAACAAGGTTGAGAAAGCTGAGGAACTTTTTCTTGCCATGTTGACATTAGGTTTGAATCCTGACGATTACACATGCAGTGCTTTTATCAAGGCATTGTGCGATTCAGGAAAACTTGATGCAGCAAAGGAGATATTGCTCTCTATGGAAGCATCTGGTTGTTTTCCGGATTCTTCTATTTGTAACAAGATTTTAGATTCGCTGGTCCAGAGTGGCCATGTTGAAGAGGCCTGTAATATAGTAAAGAGTTTTAACAGGAGGAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006091 GO:0006119 GO:0006139 GO:0006163 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0006979 GO:0008150 GO:0008152 GO:0009117 GO:0009123 GO:0009126 GO:0009141 GO:0009144 GO:0009150 GO:0009161 GO:0009167 GO:0009199 GO:0009205 GO:0009259 GO:0009451 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0015980 GO:0016070 GO:0016310 GO:0016787 GO:0016788 GO:0017144 GO:0019637 GO:0019693 GO:0022900 GO:0022904 GO:0033993 GO:0034641 GO:0042221 GO:0042773 GO:0042775 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0045333 GO:0046034 GO:0046483 GO:0050896 GO:0055086 GO:0055114 GO:0071704 GO:0072521 GO:0090304 GO:0090305 GO:0097159 GO:0097305 GO:1901135 GO:1901360 GO:1901363 GO:1901564 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

724

Amino Acids

80.65

Weight (kDa)

8.88

Isoelectric Point (pI)

36.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_long	PF17177	229 - 347	7e-10	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	236 - 281	1.5e-10	PPR repeat family
PPR_1	PF12854	264 - 296	2.2e-10	PPR repeat
PPR_2	PF13041	273 - 316	7.1e-12	PPR repeat family
PPR_1	PF12854	299 - 331	4.7e-09	PPR repeat
TPR_24	PF23276	304 - 368	1e-07	Fungal tetratrico peptide repeats
PPR_2	PF13041	306 - 349	7.1e-09	PPR repeat family
PPR_3	PF13812	435 - 492	6.3e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	449 - 495	3.1e-08	PPR repeat family
PPR_long	PF17177	464 - 560	1.5e-08	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	478 - 510	1.8e-09	PPR repeat
PPR_3	PF13812	478 - 528	1.6e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	482 - 528	5e-15	PPR repeat family
PPR	PF01535	484 - 514	2.1e-06	PPR repeat
TPR_24	PF23276	502 - 613	8.4e-07	Fungal tetratrico peptide repeats
PPR_3	PF13812	504 - 561	1.5e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	512 - 541	2.2e-07	PPR repeat
PPR_2	PF13041	516 - 565	1.9e-15	PPR repeat family
TPR_24	PF23276	537 - 646	4.1e-09	Fungal tetratrico peptide repeats
PPR_3	PF13812	546 - 596	8.2e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	548 - 580	1.3e-12	PPR repeat
PPR	PF01535	554 - 582	1.5e-06	PPR repeat
PPR_2	PF13041	558 - 599	4.8e-15	PPR repeat family
PPR_long	PF17177	567 - 681	1.5e-08	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	576 - 631	7.8e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	584 - 614	1.2e-07	PPR repeat
PPR_2	PF13041	587 - 635	2.3e-14	PPR repeat family
TPR_24	PF23276	602 - 718	4.5e-08	Fungal tetratrico peptide repeats
PPR_1	PF12854	617 - 646	4.4e-10	PPR repeat
PPR	PF01535	624 - 653	1.6e-06	PPR repeat
PPR_2	PF13041	624 - 670	1.4e-12	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0012154)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G16890 AT3G16890
fragaria_vesca	FvH4_5g29230
malus_domestica	MD00G1073300.v1.1
prunus_persica	Prupe.1G002300_v2.0.a1 Prupe.1G002300_v2.0.a1 Prupe.1G002300_v2.0.a1
pyrus_communis	pycom420g00330
rosa_chinensis	RchiOBHm_Chr7g0221991
rosa_laevigata	RLG00000002105
rosa_multiflora	Rmu_co8447505.1_g000001 Rmu_sc0004325.1_g000020
rosa_roxburghii	Rroxscaffold_3G00237520
rosa_rugosa	Rorug07G0204800
rosa_samantha	Rh7AG345400 Rh7BG335900 Rh7CG362900 Rh7DG343200
rosa_wichuraiana	Rw7G029280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	2 cut(s)	912, 1495
AccBSI	CCGCTC	1 cut(s)	1820
AccI	GTMKAC	1 cut(s)	920
AccII	CGCG	1 cut(s)	501
AccIII	TCCGGA	1 cut(s)	2071
AciI	CCGC	2 cut(s)	501, 1820
AclWI	GGATC	1 cut(s)	383
AcoI	YGGCCR	1 cut(s)	2119
AcsI	RAATTY	6 cut(s)	96, 318, 574, 672, 767, 1759
AcuI	CTGAAG	1 cut(s)	615
AfaI	GTAC	3 cut(s)	214, 1140, 1376
AfiI	CCNNNNNNNGG	4 cut(s)	525, 912, 1495, 1693
AluBI	AGCT	8 cut(s)	23, 146, 266, 1019, 1073, 1271, 1391, 1925
AluI	AGCT	8 cut(s)	23, 146, 266, 1019, 1073, 1271, 1391, 1925
Alw26I	GTCTC	4 cut(s)	92, 692, 989, 1800
AlwI	GGATC	1 cut(s)	383
Aor13HI	TCCGGA	1 cut(s)	2071
AoxI	GGCC	6 cut(s)	366, 532, 1059, 1684, 2119, 2133
ApeKI	GCWGC	2 cut(s)	38, 2027
ApoI	RAATTY	6 cut(s)	96, 318, 574, 672, 767, 1759
ArsI	GACNNNNNNTTYG	2 cut(s)	1942, 1974
Asp700I	GAANNNNTTC	2 cut(s)	145, 1935
AspLEI	GCGC	1 cut(s)	176
AspS9I	GGNCC	6 cut(s)	367, 533, 589, 797, 1060, 2110
AsuC2I	CCSGG	1 cut(s)	1338
AsuHPI	GGTGA	1 cut(s)	1834
AsuII	TTCGAA	1 cut(s)	1154
AvaII	GGWCC	3 cut(s)	589, 797, 2110
BaeGI	GKGCMC	1 cut(s)	34
BalI	TGGCCA	1 cut(s)	2121
BauI	CACGAG	1 cut(s)	356
BbsI	GAAGAC	2 cut(s)	603, 1441
BbvCI	CCTCAGC	2 cut(s)	657, 1926
BbvI	GCAGC	2 cut(s)	25, 2039
BccI	CCATC	6 cut(s)	285, 436, 929, 1676, 1729, 1772
BceAI	ACGGC	3 cut(s)	701, 1074, 1337
BcgI	CGANNNNNNTGC	2 cut(s)	598, 632
BciVI	GTATCC	1 cut(s)	1550
BclI	TGATCA	4 cut(s)	298, 720, 1414, 1422
BcnI	CCSGG	1 cut(s)	1338
BcoDI	GTCTC	4 cut(s)	92, 692, 989, 1800
BfaI	CTAG	3 cut(s)	27, 402, 1815
BfmI	CTRYAG	1 cut(s)	82
BfoI	RGCGCY	1 cut(s)	177
BfuI	GTATCC	1 cut(s)	1550
BisI	GCNGC	2 cut(s)	39, 2028
BlsI	GCNGC	2 cut(s)	40, 2029
Bme1390I	CCNGG	1 cut(s)	1338
Bme18I	GGWCC	3 cut(s)	589, 797, 2110
BmgT120I	GGNCC	6 cut(s)	367, 533, 589, 797, 1060, 2110
BmiI	GGNNCC	4 cut(s)	535, 591, 799, 1062
BmrFI	CCNGG	1 cut(s)	1338
BmrI	ACTGGG	1 cut(s)	1059
BmsI	GCATC	7 cut(s)	768, 1192, 1514, 1702, 1855, 2014, 2066
BmuI	ACTGGG	1 cut(s)	1059
BpiI	GAAGAC	2 cut(s)	603, 1441
BplI	GAGNNNNNCTC	2 cut(s)	1738, 1770
BpmI	CTGGAG	1 cut(s)	1614
Bpu10I	CCTNAGC	2 cut(s)	657, 1926
Bpu14I	TTCGAA	1 cut(s)	1154
BpuMI	CCSGG	1 cut(s)	1338
BsaI	GGTCTC	1 cut(s)	692
BsaJI	CCNNGG	1 cut(s)	1056
BsaWI	WCCGGW	4 cut(s)	525, 1577, 1701, 2071
BsaXI	ACNNNNNCTCC	2 cut(s)	622, 652
Bsc4I	CCNNNNNNNGG	4 cut(s)	525, 912, 1495, 1693
Bse1I	ACTGG	2 cut(s)	341, 1065
Bse3DI	GCAATG	2 cut(s)	1632, 1836
BseAI	TCCGGA	1 cut(s)	2071
BseDI	CCNNGG	1 cut(s)	1056
BseGI	GGATG	2 cut(s)	862, 1911
BseLI	CCNNNNNNNGG	4 cut(s)	525, 912, 1495, 1693
BseMI	GCAATG	2 cut(s)	1632, 1836
BseMII	CTCAG	6 cut(s)	103, 404, 648, 1212, 1722, 1917
BseNI	ACTGG	2 cut(s)	341, 1065
BseRI	GAGGAG	1 cut(s)	144
BseSI	GKGCMC	1 cut(s)	34
BseXI	GCAGC	2 cut(s)	25, 2039
Bsh1236I	CGCG	1 cut(s)	501
BshFI	GGCC	6 cut(s)	368, 534, 1061, 1686, 2121, 2135
BsiSI	CCGG	5 cut(s)	526, 1337, 1578, 1702, 2072
BslFI	GGGAC	3 cut(s)	575, 783, 1446
BslI	CCNNNNNNNGG	4 cut(s)	525, 912, 1495, 1693
BsmAI	GTCTC	4 cut(s)	92, 692, 989, 1800
BsmBI	CGTCTC	1 cut(s)	989
BsmFI	GGGAC	3 cut(s)	575, 783, 1446
BsnI	GGCC	6 cut(s)	368, 534, 1061, 1686, 2121, 2135
Bso31I	GGTCTC	1 cut(s)	692
Bsp119I	TTCGAA	1 cut(s)	1154
Bsp1286I	GDGCHC	1 cut(s)	34
Bsp13I	TCCGGA	1 cut(s)	2071
Bsp1407I	TGTACA	1 cut(s)	1374
Bsp143I	GATC	7 cut(s)	298, 375, 615, 720, 1028, 1414, 1422
BspACI	CCGC	2 cut(s)	501, 1820
BspANI	GGCC	6 cut(s)	368, 534, 1061, 1686, 2121, 2135
BspCNI	CTCAG	6 cut(s)	102, 403, 649, 1213, 1723, 1918
BspEI	TCCGGA	1 cut(s)	2071
BspFNI	CGCG	1 cut(s)	501
BspHI	TCATGA	1 cut(s)	1297
BspLI	GGNNCC	4 cut(s)	535, 591, 799, 1062
BspPI	GGATC	1 cut(s)	383
BspT104I	TTCGAA	1 cut(s)	1154
BspTNI	GGTCTC	1 cut(s)	692
BsrBI	CCGCTC	1 cut(s)	1820
BsrDI	GCAATG	2 cut(s)	1632, 1836
BsrGI	TGTACA	1 cut(s)	1374
BsrI	ACTGG	2 cut(s)	341, 1065
BssECI	CCNNGG	1 cut(s)	1056
BssMI	GATC	7 cut(s)	298, 375, 615, 720, 1028, 1414, 1422
BssNAI	GTATAC	1 cut(s)	921
BssSI	CACGAG	1 cut(s)	356
Bst1107I	GTATAC	1 cut(s)	921
Bst2BI	CACGAG	1 cut(s)	356
Bst4CI	ACNGT	5 cut(s)	86, 794, 1138, 1143, 1724
Bst6I	CTCTTC	2 cut(s)	1190, 2124
BstAPI	GCANNNNNTGC	1 cut(s)	38
BstAUI	TGTACA	1 cut(s)	1374
BstBI	TTCGAA	1 cut(s)	1154
BstC8I	GCNNGC	3 cut(s)	351, 783, 1845
BstDEI	CTNAG	9 cut(s)	89, 390, 508, 657, 695, 871, 1221, 1731, 1926
BstDSI	CCRYGG	1 cut(s)	1056
BstF5I	GGATG	2 cut(s)	862, 1911
BstFNI	CGCG	1 cut(s)	501
BstH2I	RGCGCY	1 cut(s)	177
BstHHI	GCGC	1 cut(s)	176
BstKTI	GATC	7 cut(s)	301, 378, 618, 723, 1031, 1417, 1425
BstMAI	GTCTC	4 cut(s)	92, 692, 989, 1800
BstMBI	GATC	7 cut(s)	298, 375, 615, 720, 1028, 1414, 1422
BstMWI	GCNNNNNNNGC	6 cut(s)	29, 38, 44, 272, 1388, 1826
BstNSI	RCATGY	2 cut(s)	38, 1983
BstSCI	CCNGG	1 cut(s)	1336
BstSFI	CTRYAG	1 cut(s)	82
BstSLI	GKGCMC	1 cut(s)	34
BstUI	CGCG	1 cut(s)	501
BstV1I	GCAGC	2 cut(s)	25, 2039
BstV2I	GAAGAC	2 cut(s)	603, 1441
BstZ17I	GTATAC	1 cut(s)	921
BsuI	GTATCC	1 cut(s)	1550
BsuRI	GGCC	6 cut(s)	368, 534, 1061, 1686, 2121, 2135
BtgI	CCRYGG	1 cut(s)	1056
BtsCI	GGATG	2 cut(s)	862, 1911
BtsI	GCAGTG	2 cut(s)	1535, 1990
BtsIMutI	CAGTG	3 cut(s)	1072, 1535, 1990
Cac8I	GCNNGC	3 cut(s)	351, 783, 1845
CciI	TCATGA	1 cut(s)	1297
CfoI	GCGC	1 cut(s)	176
Cfr13I	GGNCC	6 cut(s)	367, 533, 589, 797, 1060, 2110
Csp6I	GTAC	3 cut(s)	213, 1139, 1375
CviAII	CATG	9 cut(s)	35, 1040, 1298, 1372, 1462, 1613, 1945, 1980, 2123
CviQI	GTAC	3 cut(s)	213, 1139, 1375
DdeI	CTNAG	9 cut(s)	89, 390, 508, 657, 695, 871, 1221, 1731, 1926
DpnI	GATC	7 cut(s)	300, 377, 617, 722, 1030, 1416, 1424
DpnII	GATC	7 cut(s)	298, 375, 615, 720, 1028, 1414, 1422
EaeI	YGGCCR	1 cut(s)	2119
Eam1104I	CTCTTC	2 cut(s)	1190, 2124
EarI	CTCTTC	2 cut(s)	1190, 2124
Eco147I	AGGCCT	1 cut(s)	2135
Eco31I	GGTCTC	1 cut(s)	692
Eco47I	GGWCC	3 cut(s)	589, 797, 2110
Eco57I	CTGAAG	1 cut(s)	615
EcoO109I	RGGNCCY	1 cut(s)	533
EcoRI	GAATTC	1 cut(s)	574
Esp3I	CGTCTC	1 cut(s)	989
FaeI	CATG	9 cut(s)	38, 1043, 1301, 1375, 1465, 1616, 1948, 1983, 2126
FalI	AAGNNNNNCTT	6 cut(s)	434, 466, 588, 620, 1419, 1451
FaqI	GGGAC	3 cut(s)	575, 783, 1446
FatI	CATG	9 cut(s)	34, 1039, 1297, 1371, 1461, 1612, 1944, 1979, 2122
FbaI	TGATCA	4 cut(s)	298, 720, 1414, 1422
FblI	GTMKAC	1 cut(s)	920
Fnu4HI	GCNGC	2 cut(s)	39, 2028
FokI	GGATG	2 cut(s)	869, 1918
Fsp4HI	GCNGC	2 cut(s)	39, 2028
FspBI	CTAG	3 cut(s)	27, 402, 1815
GlaI	GCGC	1 cut(s)	175
GluI	GCNGC	2 cut(s)	39, 2028
GsuI	CTGGAG	1 cut(s)	1614
HaeII	RGCGCY	1 cut(s)	177
HaeIII	GGCC	6 cut(s)	368, 534, 1061, 1686, 2121, 2135
HapII	CCGG	5 cut(s)	526, 1337, 1578, 1702, 2072
HhaI	GCGC	1 cut(s)	176
Hin1II	CATG	9 cut(s)	38, 1043, 1301, 1375, 1465, 1616, 1948, 1983, 2126
Hin6I	GCGC	1 cut(s)	174
HinP1I	GCGC	1 cut(s)	174
HincII	GTYRAC	1 cut(s)	1950
HindII	GTYRAC	1 cut(s)	1950
HindIII	AAGCTT	2 cut(s)	144, 1071
HpaII	CCGG	5 cut(s)	526, 1337, 1578, 1702, 2072
HphI	GGTGA	1 cut(s)	1834
Hpy166II	GTNNAC	4 cut(s)	921, 1662, 1673, 1950
Hpy188I	TCNGA	6 cut(s)	53, 102, 1028, 1281, 1287, 1444
Hpy188III	TCNNGA	7 cut(s)	800, 1298, 1815, 1967, 2013, 2072, 2113
Hpy8I	GTNNAC	4 cut(s)	921, 1662, 1673, 1950
HpyAV	CCTTC	3 cut(s)	325, 358, 979
HpyCH4III	ACNGT	5 cut(s)	86, 794, 1138, 1143, 1724
HpyCH4IV	ACGT	1 cut(s)	917
HpyF10VI	GCNNNNNNNGC	6 cut(s)	29, 38, 44, 272, 1388, 1826
HpyF3I	CTNAG	9 cut(s)	89, 390, 508, 657, 695, 871, 1221, 1731, 1926
HpySE526I	ACGT	1 cut(s)	917
Hsp92II	CATG	9 cut(s)	38, 1043, 1301, 1375, 1465, 1616, 1948, 1983, 2126
HspAI	GCGC	1 cut(s)	174
Kpn2I	TCCGGA	1 cut(s)	2071
Ksp22I	TGATCA	4 cut(s)	298, 720, 1414, 1422
Kzo9I	GATC	7 cut(s)	298, 375, 615, 720, 1028, 1414, 1422
LmnI	GCTCC	2 cut(s)	157, 1276
Lsp1109I	GCAGC	2 cut(s)	25, 2039
LweI	GCATC	7 cut(s)	768, 1192, 1514, 1702, 1855, 2014, 2066
MaeI	CTAG	3 cut(s)	27, 402, 1815
MaeII	ACGT	1 cut(s)	917
MaeIII	GTNAC	6 cut(s)	464, 1003, 1394, 1555, 1750, 2087
MalI	GATC	7 cut(s)	300, 377, 617, 722, 1030, 1416, 1424
MbiI	CCGCTC	1 cut(s)	1820
MboI	GATC	7 cut(s)	298, 375, 615, 720, 1028, 1414, 1422
MfeI	CAATTG	1 cut(s)	294
MhlI	GDGCHC	1 cut(s)	34
MlsI	TGGCCA	1 cut(s)	2121
MluNI	TGGCCA	1 cut(s)	2121
MlyI	GAGTC	3 cut(s)	843, 1000, 1115
MmeI	TCCRAC	3 cut(s)	796, 1476, 1783
Mox20I	TGGCCA	1 cut(s)	2121
MroI	TCCGGA	1 cut(s)	2071
MroXI	GAANNNNTTC	2 cut(s)	145, 1935
MscI	TGGCCA	1 cut(s)	2121
MseI	TTAA	6 cut(s)	570, 584, 876, 1647, 1878, 2157
MslI	CAYNNNNRTG	1 cut(s)	1244
Msp20I	TGGCCA	1 cut(s)	2121
MspA1I	CMGCKG	1 cut(s)	266
MspI	CCGG	5 cut(s)	526, 1337, 1578, 1702, 2072
MspR9I	CCNGG	1 cut(s)	1338
MunI	CAATTG	1 cut(s)	294
MvnI	CGCG	1 cut(s)	501
MwoI	GCNNNNNNNGC	6 cut(s)	29, 38, 44, 272, 1388, 1826
NciI	CCSGG	1 cut(s)	1338
NdeII	GATC	7 cut(s)	298, 375, 615, 720, 1028, 1414, 1422
NlaIII	CATG	9 cut(s)	38, 1043, 1301, 1375, 1465, 1616, 1948, 1983, 2126
NlaIV	GGNNCC	4 cut(s)	535, 591, 799, 1062
NmuCI	GTSAC	3 cut(s)	1003, 1394, 1750
NspI	RCATGY	2 cut(s)	38, 1983
NspV	TTCGAA	1 cut(s)	1154
PagI	TCATGA	1 cut(s)	1297
PceI	AGGCCT	1 cut(s)	2135
PcsI	WCGNNNNNNNCGW	1 cut(s)	163
PdmI	GAANNNNTTC	2 cut(s)	145, 1935
PflMI	CCANNNNNTGG	2 cut(s)	912, 1495
PkrI	GCNGC	2 cut(s)	40, 2029
PleI	GAGTC	3 cut(s)	842, 1000, 1114
PpsI	GAGTC	3 cut(s)	842, 1000, 1114
PspN4I	GGNNCC	4 cut(s)	535, 591, 799, 1062
PspPI	GGNCC	6 cut(s)	367, 533, 589, 797, 1060, 2110
PvuII	CAGCTG	1 cut(s)	266
RsaI	GTAC	3 cut(s)	214, 1140, 1376
RsaNI	GTAC	3 cut(s)	213, 1139, 1375
RseI	CAYNNNNRTG	1 cut(s)	1244
SaqAI	TTAA	6 cut(s)	570, 584, 876, 1647, 1878, 2157
SatI	GCNGC	2 cut(s)	39, 2028
Sau3AI	GATC	7 cut(s)	298, 375, 615, 720, 1028, 1414, 1422
Sau96I	GGNCC	6 cut(s)	367, 533, 589, 797, 1060, 2110
SchI	GAGTC	3 cut(s)	843, 1000, 1115
ScrFI	CCNGG	1 cut(s)	1338
SduI	GDGCHC	1 cut(s)	34
SfaNI	GCATC	7 cut(s)	768, 1192, 1514, 1702, 1855, 2014, 2066
SfcI	CTRYAG	1 cut(s)	82
SfuI	TTCGAA	1 cut(s)	1154
SinI	GGWCC	3 cut(s)	589, 797, 2110
SmiMI	CAYNNNNRTG	1 cut(s)	1244
SseBI	AGGCCT	1 cut(s)	2135
SsiI	CCGC	2 cut(s)	501, 1820
SspI	AATATT	1 cut(s)	650
SspMI	CTAG	3 cut(s)	27, 402, 1815
StuI	AGGCCT	1 cut(s)	2135
StyD4I	CCNGG	1 cut(s)	1336
TaaI	ACNGT	5 cut(s)	86, 794, 1138, 1143, 1724
TaiI	ACGT	1 cut(s)	920
TaqI	TCGA	3 cut(s)	166, 461, 1154
TatI	WGTACW	1 cut(s)	1374
Tru1I	TTAA	6 cut(s)	570, 584, 876, 1647, 1878, 2157
Tru9I	TTAA	6 cut(s)	570, 584, 876, 1647, 1878, 2157
TscAI	CASTG	3 cut(s)	1072, 1535, 1990
TseFI	GTSAC	3 cut(s)	1003, 1394, 1750
TseI	GCWGC	2 cut(s)	38, 2027
Tsp45I	GTSAC	3 cut(s)	1003, 1394, 1750
TspDTI	ATGAA	8 cut(s)	105, 666, 1028, 1029, 1286, 1629, 1847, 1922
TspGWI	ACGGA	3 cut(s)	343, 484, 680
TspRI	CASTG	3 cut(s)	1072, 1535, 1990
Van91I	CCANNNNNTGG	2 cut(s)	912, 1495
VpaK11BI	GGWCC	3 cut(s)	589, 797, 2110
XapI	RAATTY	6 cut(s)	96, 318, 574, 672, 767, 1759
XbaI	TCTAGA	1 cut(s)	1814
XceI	RCATGY	2 cut(s)	38, 1983
XmiI	GTMKAC	1 cut(s)	920
XmnI	GAANNNNTTC	2 cut(s)	145, 1935
XspI	CTAG	3 cut(s)	27, 402, 1815

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.