MD00G1083800.v1.1

lysM domain-containing GPI-anchored protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0012394
Physical Location & Seq
Reverse (-)
16671749 .. 16674834
3086 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1083800.v1.1.491

Sequence Viewer

Length: 1071 bp
ATGTCCACACTCGCACTTCTCCTCACCCTCTCCCTCCTCTCATTTCTCCCCACACCTACCTCCTCCGCCACCTTCAACTGCTCCGTCCCCGCCTCCAACACCTGCCGCTCCCTCATCGCCTACATCCCCCCTAACTCCACCACCCTTAACTCCATCAAAACCCTCTTCAACGTCACCCACTTCCGCACCCTGCTCGGCGCCAACAACTTGCCCATCGCCACCGACCCGGCCTTACCCGTCTCCGCTAGACAAAAGGTCCTCGTCCCCTTCACCTGCGTCTGCCGCAACGGCACTGGCGTCTCCAACAAGCGGCCTCAGTACATAGTCAAGTCCGGCGACGACCTCGACCGCATAGCCACCGTCCTATTCTCGAGGCTCGTGACGTACCAGGAAATCGCGGCGGTGAATAAGATATCCAACCCCAGTCTGATTCAGATCGGGCAGAAGCTGTGGATTCCGCTGCCGTGTAGCTGCGATGAAGTGGACGGTGAGAGGGTGGTCCATTACGGACACGTGGTGGCGAATGGGAGCTCGGTGGAGGCGATTGCGACGGAGTACGGGACGACCCAGGAGACTCTGCTGAGGATCAATGGGATATCGGATCCGAAGACCCTTAAGGCCGATGAAGTCCTCGATGTTCCTCTCAAAGCTTGTTCGTCTTCCATATCCAATGAATCATCTGACGCCCGCTTACTGGTTTCTAATAACACCTACGTCTTTACTGCCAACGATTGCGTGAAGTGCCAATGCAGTTCTGCAAACAATTGGACCTTACAGTGCGAGCCATCCACCGTTAACTCAACTGTATGCCCTGCGATGCAATGCGAGGATTCAAGTCTGACTAGTCAGCTATTACTTGGCAACAGCACATCCGGTTGCAATCAAACCACCTGTGCCTATGCTGGTTATACTAACCAGACCAAGATTCTTACAACCTTGGTAACACACTCCACTTGTACAGCTTCGGATAATAAAAATAACGCTTCAAAGATGAGTTTGCAAGGCTCGAGTTGGAACTTCCTTTTCGTGGCAATTCACTTGGTGCTTCTCTTTCTTCATGTTCTTCAATGA

Protein Analysis

357

Amino Acids

38.3

Weight (kDa)

6.95

Isoelectric Point (pI)

36.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LysM2_NFP PF23457 106 - 154 2.2e-06 NFP LysM2 domain
LysM PF01476 107 - 153 1.3e-10 LysM domain
LysM PF01476 171 - 214 7.9e-09 LysM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012394)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G17120
fragaria_vesca FvH4_2g16381
malus_domestica MD00G1083800.v1.1
prunus_persica Prupe.8G176700_v2.0.a1 Prupe.8G176700_v2.0.a1
pyrus_communis pycom05g12980
rosa_chinensis RchiOBHm_Chr6g0280171
rosa_laevigata RLG00000013080
rosa_multiflora Rmu_co8435311.1_g000001 Rmu_sc0007137.1_g000014
rosa_roxburghii Rroxscaffold_7G00188340
rosa_rugosa Rorug06G0130300 Rorug06G0130400
rosa_samantha Rh6AG240700 Rh6AG241500 Rh6BG244400 Rh6CG246000 Rh6DG237100
rosa_wichuraiana Rw6G020970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 110, 281
Acc36I ACCTGC 2 cut(s) 110, 281
AccB1I GGYRCC 1 cut(s) 197
AccBSI CCGCTC 1 cut(s) 108
AccII CGCG 1 cut(s) 398
AclWI GGATC 3 cut(s) 593, 596, 609
AcvI CACGTG 1 cut(s) 514
AcyI GRCGYC 3 cut(s) 198, 297, 684
AdeI CACNNNGTG 2 cut(s) 517, 1042
AfaI GTAC 4 cut(s) 320, 386, 557, 958
AfiI CCNNNNNNNGG 3 cut(s) 309, 694, 1027
AflII CTTAAG 1 cut(s) 614
AflIII ACRYGT 1 cut(s) 511
AgsI TTSAA 5 cut(s) 76, 169, 834, 987, 1067
AhdI GACNNNNNGTC 1 cut(s) 254
AhlI ACTAGT 1 cut(s) 842
AjnI CCWGG 2 cut(s) 387, 567
AluBI AGCT 6 cut(s) 448, 471, 531, 650, 850, 962
AluI AGCT 6 cut(s) 448, 471, 531, 650, 850, 962
Alw21I GWGCWC 1 cut(s) 533
Alw26I GTCTC 3 cut(s) 244, 304, 566
AlwI GGATC 3 cut(s) 593, 596, 609
AlwNI CAGNNNCTG 1 cut(s) 448
Ama87I CYCGRG 2 cut(s) 370, 1006
AoxI GGCC 3 cut(s) 228, 311, 618
ApeKI GCWGC 2 cut(s) 460, 471
AspLEI GCGC 1 cut(s) 200
AspS9I GGNCC 3 cut(s) 256, 499, 768
AsuC2I CCSGG 1 cut(s) 227
AsuHPI GGTGA 5 cut(s) 16, 166, 262, 415, 500
AvaI CYCGRG 2 cut(s) 370, 1006
AvaII GGWCC 3 cut(s) 256, 499, 768
BamHI GGATCC 1 cut(s) 601
BanI GGYRCC 1 cut(s) 197
BanII GRGCYC 1 cut(s) 533
BauI CACGAG 1 cut(s) 377
BbrPI CACGTG 1 cut(s) 514
BbsI GAAGAC 2 cut(s) 614, 651
Bbv12I GWGCWC 1 cut(s) 533
BbvCI CCTCAGC 1 cut(s) 581
BbvI GCAGC 2 cut(s) 447, 458
BccI CCATC 3 cut(s) 161, 221, 793
BceAI ACGGC 2 cut(s) 304, 448
BcgI CGANNNNNNTGC 2 cut(s) 175, 209
BciT130I CCWGG 2 cut(s) 389, 569
BcnI CCSGG 1 cut(s) 227
BcoDI GTCTC 3 cut(s) 244, 304, 566
BcuI ACTAGT 1 cut(s) 842
BfaI CTAG 2 cut(s) 246, 843
BfoI RGCGCY 1 cut(s) 201
BfrI CTTAAG 1 cut(s) 614
BfuAI ACCTGC 2 cut(s) 110, 281
BglI GCCNNNNNGGC 1 cut(s) 288
BisI GCNGC 6 cut(s) 106, 283, 311, 399, 461, 472
BlsI GCNGC 6 cut(s) 107, 284, 312, 400, 462, 473
Bme1390I CCNGG 3 cut(s) 227, 389, 569
Bme18I GGWCC 3 cut(s) 256, 499, 768
BmeRI GACNNNNNGTC 1 cut(s) 254
BmeT110I CYCGRG 2 cut(s) 370, 1006
BmgT120I GGNCC 3 cut(s) 256, 499, 768
BmiI GGNNCC 2 cut(s) 199, 603
BmrFI CCNGG 3 cut(s) 227, 389, 569
BmrI ACTGGG 1 cut(s) 417
BmsI GCATC 1 cut(s) 807
BmuI ACTGGG 1 cut(s) 417
BpiI GAAGAC 2 cut(s) 614, 651
Bpu10I CCTNAGC 1 cut(s) 581
BpuMI CCSGG 1 cut(s) 227
BsaAI YACGTR 1 cut(s) 514
BsaBI GATNNNNATC 1 cut(s) 434
BsaHI GRCGYC 3 cut(s) 198, 297, 684
BsaJI CCNNGG 2 cut(s) 567, 936
BsaWI WCCGGW 1 cut(s) 872
Bsc4I CCNNNNNNNGG 3 cut(s) 309, 694, 1027
Bse1I ACTGG 3 cut(s) 298, 423, 699
Bse3DI GCAATG 1 cut(s) 827
Bse8I GATNNNNATC 1 cut(s) 434
BseBI CCWGG 2 cut(s) 389, 569
BseDI CCNNGG 2 cut(s) 567, 936
BseGI GGATG 3 cut(s) 123, 785, 869
BseJI GATNNNNATC 1 cut(s) 434
BseLI CCNNNNNNNGG 3 cut(s) 309, 694, 1027
BseMI GCAATG 1 cut(s) 827
BseMII CTCAG 2 cut(s) 329, 572
BseNI ACTGG 3 cut(s) 298, 423, 699
BseRI GAGGAG 3 cut(s) 11, 26, 52
BseXI GCAGC 2 cut(s) 447, 458
Bsh1236I CGCG 1 cut(s) 398
Bsh1285I CGRYCG 1 cut(s) 349
BshFI GGCC 3 cut(s) 230, 313, 620
BshNI GGYRCC 1 cut(s) 197
BsiEI CGRYCG 1 cut(s) 349
BsiHKAI GWGCWC 1 cut(s) 533
BsiHKCI CYCGRG 2 cut(s) 370, 1006
BsiSI CCGG 3 cut(s) 227, 333, 873
BslFI GGGAC 3 cut(s) 71, 248, 574
BslI CCNNNNNNNGG 3 cut(s) 309, 694, 1027
BsmAI GTCTC 3 cut(s) 244, 304, 566
BsmBI CGTCTC 2 cut(s) 244, 304
BsmFI GGGAC 3 cut(s) 71, 248, 574
BsnI GGCC 3 cut(s) 230, 313, 620
BsoBI CYCGRG 2 cut(s) 370, 1006
Bsp1286I GDGCHC 1 cut(s) 533
Bsp1407I TGTACA 1 cut(s) 956
Bsp143I GATC 3 cut(s) 435, 585, 601
BspANI GGCC 3 cut(s) 230, 313, 620
BspCNI CTCAG 2 cut(s) 328, 573
BspFNI CGCG 1 cut(s) 398
BspLI GGNNCC 2 cut(s) 199, 603
BspMI ACCTGC 2 cut(s) 110, 281
BspPI GGATC 3 cut(s) 593, 596, 609
BspT107I GGYRCC 1 cut(s) 197
BspTI CTTAAG 1 cut(s) 614
BsrBI CCGCTC 1 cut(s) 108
BsrDI GCAATG 1 cut(s) 827
BsrGI TGTACA 1 cut(s) 956
BsrI ACTGG 3 cut(s) 298, 423, 699
BssECI CCNNGG 2 cut(s) 567, 936
BssMI GATC 3 cut(s) 435, 585, 601
BssNI GRCGYC 3 cut(s) 198, 297, 684
BssSI CACGAG 1 cut(s) 377
BssT1I CCWWGG 1 cut(s) 936
Bst2BI CACGAG 1 cut(s) 377
Bst2UI CCWGG 2 cut(s) 389, 569
Bst4CI ACNGT 5 cut(s) 361, 488, 777, 793, 805
Bst6I CTCTTC 1 cut(s) 170
BstACI GRCGYC 3 cut(s) 198, 297, 684
BstAFI CTTAAG 1 cut(s) 614
BstAUI TGTACA 1 cut(s) 956
BstBAI YACGTR 1 cut(s) 514
BstC8I GCNNGC 2 cut(s) 688, 782
BstDEI CTNAG 2 cut(s) 315, 581
BstF5I GGATG 3 cut(s) 123, 785, 869
BstFNI CGCG 1 cut(s) 398
BstH2I RGCGCY 1 cut(s) 201
BstHHI GCGC 1 cut(s) 200
BstKTI GATC 3 cut(s) 438, 588, 604
BstMAI GTCTC 3 cut(s) 244, 304, 566
BstMBI GATC 3 cut(s) 435, 585, 601
BstMCI CGRYCG 1 cut(s) 349
BstMWI GCNNNNNNNGC 3 cut(s) 282, 288, 741
BstNI CCWGG 2 cut(s) 389, 569
BstSCI CCNGG 3 cut(s) 225, 387, 567
BstUI CGCG 1 cut(s) 398
BstV1I GCAGC 2 cut(s) 447, 458
BstV2I GAAGAC 2 cut(s) 614, 651
BstX2I RGATCY 1 cut(s) 601
BstYI RGATCY 1 cut(s) 601
BsuRI GGCC 3 cut(s) 230, 313, 620
BtgZI GCGATG 4 cut(s) 100, 199, 489, 830
BtsCI GGATG 3 cut(s) 123, 785, 869
BtsIMutI CAGTG 2 cut(s) 291, 782
BveI ACCTGC 2 cut(s) 110, 281
Cac8I GCNNGC 2 cut(s) 688, 782
CaiI CAGNNNCTG 1 cut(s) 448
CfoI GCGC 1 cut(s) 200
Cfr13I GGNCC 3 cut(s) 256, 499, 768
CseI GACGC 3 cut(s) 265, 286, 692
Csp6I GTAC 4 cut(s) 319, 385, 556, 957
CviAII CATG 1 cut(s) 1058
CviQI GTAC 4 cut(s) 319, 385, 556, 957
DdeI CTNAG 2 cut(s) 315, 581
DinI GGCGCC 1 cut(s) 199
DpnI GATC 3 cut(s) 437, 587, 603
DpnII GATC 3 cut(s) 435, 585, 601
DraIII CACNNNGTG 2 cut(s) 517, 1042
DriI GACNNNNNGTC 1 cut(s) 254
Eam1104I CTCTTC 1 cut(s) 170
Eam1105I GACNNNNNGTC 1 cut(s) 254
EarI CTCTTC 1 cut(s) 170
EciI GGCGGA 1 cut(s) 55
Ecl136II GAGCTC 1 cut(s) 531
Eco130I CCWWGG 1 cut(s) 936
Eco24I GRGCYC 1 cut(s) 533
Eco32I GATATC 2 cut(s) 414, 597
Eco47I GGWCC 3 cut(s) 256, 499, 768
Eco53kI GAGCTC 1 cut(s) 531
Eco72I CACGTG 1 cut(s) 514
Eco88I CYCGRG 2 cut(s) 370, 1006
EcoICRI GAGCTC 1 cut(s) 531
EcoO109I RGGNCCY 1 cut(s) 256
EcoRII CCWGG 2 cut(s) 387, 567
EcoRV GATATC 2 cut(s) 414, 597
EcoT14I CCWWGG 1 cut(s) 936
EcoT38I GRGCYC 1 cut(s) 533
EgeI GGCGCC 1 cut(s) 199
EheI GGCGCC 1 cut(s) 199
ErhI CCWWGG 1 cut(s) 936
Esp3I CGTCTC 2 cut(s) 244, 304
FaeI CATG 1 cut(s) 1061
FaiI YATR 7 cut(s) 323, 353, 665, 808, 900, 909, 1059
FaqI GGGAC 3 cut(s) 71, 248, 574
FatI CATG 1 cut(s) 1057
FauI CCCGC 2 cut(s) 97, 695
Fnu4HI GCNGC 6 cut(s) 106, 283, 311, 399, 461, 472
FokI GGATG 3 cut(s) 110, 772, 856
FriOI GRGCYC 1 cut(s) 533
Fsp4HI GCNGC 6 cut(s) 106, 283, 311, 399, 461, 472
FspBI CTAG 2 cut(s) 246, 843
GlaI GCGC 1 cut(s) 199
GluI GCNGC 6 cut(s) 106, 283, 311, 399, 461, 472
HaeII RGCGCY 1 cut(s) 201
HaeIII GGCC 3 cut(s) 230, 313, 620
HapII CCGG 3 cut(s) 227, 333, 873
HgaI GACGC 3 cut(s) 265, 286, 692
HhaI GCGC 1 cut(s) 200
Hin1I GRCGYC 3 cut(s) 198, 297, 684
Hin1II CATG 1 cut(s) 1061
Hin6I GCGC 1 cut(s) 198
HinP1I GCGC 1 cut(s) 198
HincII GTYRAC 1 cut(s) 796
HindII GTYRAC 1 cut(s) 796
HindIII AAGCTT 1 cut(s) 648
HinfI GANTC 6 cut(s) 430, 454, 574, 674, 830, 925
HpaI GTTAAC 1 cut(s) 796
HpaII CCGG 3 cut(s) 227, 333, 873
HphI GGTGA 5 cut(s) 16, 166, 262, 415, 500
Hpy166II GTNNAC 3 cut(s) 6, 484, 796
Hpy188I TCNGA 7 cut(s) 429, 435, 601, 606, 682, 840, 967
Hpy188III TCNNGA 2 cut(s) 370, 379
Hpy8I GTNNAC 3 cut(s) 6, 484, 796
Hpy99I CGWCG 2 cut(s) 341, 553
HpyAV CCTTC 2 cut(s) 82, 277
HpyCH4III ACNGT 5 cut(s) 361, 488, 777, 793, 805
HpyCH4IV ACGT 4 cut(s) 171, 383, 513, 714
HpyCH4V TGCA 5 cut(s) 750, 758, 820, 879, 1000
HpyF10VI GCNNNNNNNGC 3 cut(s) 282, 288, 741
HpyF3I CTNAG 2 cut(s) 315, 581
HpySE526I ACGT 4 cut(s) 171, 383, 513, 714
Hsp92I GRCGYC 3 cut(s) 198, 297, 684
Hsp92II CATG 1 cut(s) 1061
HspAI GCGC 1 cut(s) 198
KasI GGCGCC 1 cut(s) 197
KspAI GTTAAC 1 cut(s) 796
Kzo9I GATC 3 cut(s) 435, 585, 601
LmnI GCTCC 3 cut(s) 86, 113, 528
Lsp1109I GCAGC 2 cut(s) 447, 458
LweI GCATC 1 cut(s) 807
MaeI CTAG 2 cut(s) 246, 843
MaeII ACGT 4 cut(s) 171, 383, 513, 714
MaeIII GTNAC 3 cut(s) 172, 379, 940
MalI GATC 3 cut(s) 437, 587, 603
MbiI CCGCTC 1 cut(s) 108
MboI GATC 3 cut(s) 435, 585, 601
MboII GAAGA 5 cut(s) 157, 619, 651, 1046, 1055
MfeI CAATTG 1 cut(s) 763
MflI RGATCY 1 cut(s) 601
MhlI GDGCHC 1 cut(s) 533
MluCI AATT 2 cut(s) 763, 1032
Mly113I GGCGCC 1 cut(s) 198
MlyI GAGTC 1 cut(s) 568
MmeI TCCRAC 4 cut(s) 120, 327, 441, 992
MseI TTAA 3 cut(s) 147, 615, 795
MspA1I CMGCKG 1 cut(s) 460
MspCI CTTAAG 1 cut(s) 614
MspI CCGG 3 cut(s) 227, 333, 873
MspR9I CCNGG 3 cut(s) 227, 389, 569
MunI CAATTG 1 cut(s) 763
MvaI CCWGG 2 cut(s) 389, 569
MvnI CGCG 1 cut(s) 398
MwoI GCNNNNNNNGC 3 cut(s) 282, 288, 741
NarI GGCGCC 1 cut(s) 198
NciI CCSGG 1 cut(s) 227
NdeII GATC 3 cut(s) 435, 585, 601
NlaIII CATG 1 cut(s) 1061
NlaIV GGNNCC 2 cut(s) 199, 603
NmeAIII GCCGAG 1 cut(s) 174
NmuCI GTSAC 2 cut(s) 172, 379
PaeR7I CTCGAG 2 cut(s) 370, 1006
PaqCI CACCTGC 2 cut(s) 110, 281
PcsI WCGNNNNNNNCGW 2 cut(s) 294, 539
PfeI GAWTC 5 cut(s) 430, 454, 674, 830, 925
PkrI GCNGC 6 cut(s) 107, 284, 312, 400, 462, 473
PleI GAGTC 1 cut(s) 568
PluTI GGCGCC 1 cut(s) 201
PmaCI CACGTG 1 cut(s) 514
PmlI CACGTG 1 cut(s) 514
PpsI GAGTC 1 cut(s) 568
Ppu21I YACGTR 1 cut(s) 514
PpuMI RGGWCCY 1 cut(s) 256
Psp124BI GAGCTC 1 cut(s) 533
Psp5II RGGWCCY 1 cut(s) 256
Psp6I CCWGG 2 cut(s) 387, 567
PspCI CACGTG 1 cut(s) 514
PspGI CCWGG 2 cut(s) 387, 567
PspN4I GGNNCC 2 cut(s) 199, 603
PspPI GGNCC 3 cut(s) 256, 499, 768
PspPPI RGGWCCY 1 cut(s) 256
PspXI VCTCGAGB 1 cut(s) 1006
PstNI CAGNNNCTG 1 cut(s) 448
PsuI RGATCY 1 cut(s) 601
RsaI GTAC 4 cut(s) 320, 386, 557, 958
RsaNI GTAC 4 cut(s) 319, 385, 556, 957
SacI GAGCTC 1 cut(s) 533
SaqAI TTAA 3 cut(s) 147, 615, 795
SatI GCNGC 6 cut(s) 106, 283, 311, 399, 461, 472
Sau3AI GATC 3 cut(s) 435, 585, 601
Sau96I GGNCC 3 cut(s) 256, 499, 768
SchI GAGTC 1 cut(s) 568
ScrFI CCNGG 3 cut(s) 227, 389, 569
SduI GDGCHC 1 cut(s) 533
SfaNI GCATC 1 cut(s) 807
SfoI GGCGCC 1 cut(s) 199
Sfr274I CTCGAG 2 cut(s) 370, 1006
SinI GGWCC 3 cut(s) 256, 499, 768
SlaI CTCGAG 2 cut(s) 370, 1006
SmlI CTYRAG 3 cut(s) 370, 614, 1006
SmoI CTYRAG 3 cut(s) 370, 614, 1006
SpeI ACTAGT 1 cut(s) 842
Sse9I AATT 2 cut(s) 763, 1032
SspDI GGCGCC 1 cut(s) 197
SspMI CTAG 2 cut(s) 246, 843
SstI GAGCTC 1 cut(s) 533
StyD4I CCNGG 3 cut(s) 225, 387, 567
StyI CCWWGG 1 cut(s) 936
TaaI ACNGT 5 cut(s) 361, 488, 777, 793, 805
TaiI ACGT 4 cut(s) 174, 386, 516, 717
TaqI TCGA 4 cut(s) 345, 371, 633, 1007
TasI AATT 2 cut(s) 763, 1032
TatI WGTACW 2 cut(s) 318, 956
TauI GCSGC 4 cut(s) 108, 285, 313, 401
TfiI GAWTC 5 cut(s) 430, 454, 674, 830, 925
Tru1I TTAA 3 cut(s) 147, 615, 795
Tru9I TTAA 3 cut(s) 147, 615, 795
TscAI CASTG 2 cut(s) 298, 782
TseFI GTSAC 2 cut(s) 172, 379
TseI GCWGC 2 cut(s) 460, 471
Tsp45I GTSAC 2 cut(s) 172, 379
TspDTI ATGAA 4 cut(s) 492, 639, 687, 1046
TspGWI ACGGA 3 cut(s) 73, 522, 566
TspRI CASTG 2 cut(s) 298, 782
Vha464I CTTAAG 1 cut(s) 614
VpaK11BI GGWCC 3 cut(s) 256, 499, 768
XhoI CTCGAG 2 cut(s) 370, 1006
XspI CTAG 2 cut(s) 246, 843
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.