RchiOBHm_Chr6g0280171

lysM domain-containing GPI-anchored protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0012394
Physical Location & Seq
Forward (+)
43464282 .. 43467254
2973 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ25125

Sequence Viewer

Length: 1062 bp
ATGAGTTTCTCTAACCTCACCCTGCTCCTCACCCTCGCCCTCCTCATCTCAACCCTCACCGCCCCCTCCTCCGCCGCCACCTTCAACTGCACTACCCCCTCCGCCAATTCCACCTGCCAATCCCTCATCGACTTCGCCCCCATAAACGCCACTACTCTCAACGCCGTCAAGACCCTCTTCAACATCACCCACCTCCGCACCTTACTCGGAGCCAACAACCTCCCCCTCTCGACCCGACCCAACGAACCCGTCACCGCCAACCAGAAGCTCCGAATCCCCTTCTCCTGCCGCTGCTCCAACGGCTCCGGCGTCTCCTACCACCGCCCCCAGTACATCGTCAAGGCCGGCGACGGCCTCGACTACATAGCCACCAGCTTGTTCTCGCGCCTGGTCACGTACCAGGAGATCGCCCGGGTCAATGGCATACCCGACCCGAATAAGATCGAGGTCGGGCAGAAGCTGTGGATCCCACTGCCTTGTAGCTGCGACAAAGTGGACGGCGAGGAAGTAGTGCATTACGGACACGTGGTGGACCCTGGGAGCACGGTGCAGGGGATCGCCGAGGAGTATGGGACCACCCAAGAGACGCTCCTTCGGATCAATGGGATGCCTGACCCCAATGAACTCGAGGCTGGTCAGGTCCTTGATGTTCCTCTCAAAGCTTGTGCATCGTCAGTACGAGATGACTCGATAGATTCCCCGTTACTTGTTCCTAATAACACCTATGTCTTCACTGCTTTCAATTGTGTGAAGTGCCAATGTAGTTCTGCGAACAACTGGAAATTACAATGCGAGCCATCAAATCTTAATTCGAAATGCCCTTCGATGCAATGCCAAGATTCAAATCAATTATACATTGGCAACACCACATCTTCCTCCTCCTGCAATCAAACAACTTGTGCCTATAGGGGGTACGATAACCAAACCATTTTTACAGATCTGGTAGCTCAGTCCACCTGTTCAACTCCTAGCATCGCTTCAAAGATGGGATTACACAGCTGGAGTTGGAACTTCCTTTTCGTCTCAATTCACTTGGCTCTTCTTTGTCTTCATCTTCTATGA

Protein Analysis

353

Amino Acids

38.35

Weight (kDa)

5.78

Isoelectric Point (pI)

39.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LysM2_NFP PF23457 105 - 158 1.2e-06 NFP LysM2 domain
LysM PF01476 111 - 157 1.1e-10 LysM domain
LysM PF01476 175 - 218 6e-11 LysM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012394)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G17120
fragaria_vesca FvH4_2g16381
malus_domestica MD00G1083800.v1.1
prunus_persica Prupe.8G176700_v2.0.a1 Prupe.8G176700_v2.0.a1
pyrus_communis pycom05g12980
rosa_chinensis RchiOBHm_Chr6g0280171
rosa_laevigata RLG00000013080
rosa_multiflora Rmu_co8435311.1_g000001 Rmu_sc0007137.1_g000014
rosa_roxburghii Rroxscaffold_7G00188340
rosa_rugosa Rorug06G0130300 Rorug06G0130400
rosa_samantha Rh6AG240700 Rh6AG241500 Rh6BG244400 Rh6CG246000 Rh6DG237100
rosa_wichuraiana Rw6G020970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 122
Acc36I ACCTGC 1 cut(s) 122
AccII CGCG 1 cut(s) 385
AciI CCGC 8 cut(s) 60, 72, 75, 102, 196, 255, 289, 322
AclWI GGATC 4 cut(s) 460, 473, 563, 605
AcvI CACGTG 1 cut(s) 526
AcyI GRCGYC 1 cut(s) 309
AdeI CACNNNGTG 1 cut(s) 529
AfaI GTAC 4 cut(s) 332, 398, 678, 914
AfiI CCNNNNNNNGG 1 cut(s) 909
AflIII ACRYGT 1 cut(s) 523
AgsI TTSAA 6 cut(s) 85, 181, 742, 843, 963, 981
AjnI CCWGG 3 cut(s) 387, 399, 535
AluBI AGCT 7 cut(s) 268, 375, 460, 483, 662, 947, 999
AluI AGCT 7 cut(s) 268, 375, 460, 483, 662, 947, 999
Alw21I GWGCWC 1 cut(s) 545
Alw26I GTCTC 3 cut(s) 316, 578, 1027
AlwI GGATC 4 cut(s) 460, 473, 563, 605
AlwNI CAGNNNCTG 1 cut(s) 460
Ama87I CYCGRG 2 cut(s) 411, 626
AoxI GGCC 2 cut(s) 342, 352
ApeKI GCWGC 2 cut(s) 291, 483
AspLEI GCGC 1 cut(s) 387
AspS9I GGNCC 3 cut(s) 532, 573, 640
AsuC2I CCSGG 2 cut(s) 412, 413
AsuHPI GGTGA 5 cut(s) 10, 22, 49, 178, 244
AsuII TTCGAA 1 cut(s) 812
AvaI CYCGRG 2 cut(s) 411, 626
AvaII GGWCC 3 cut(s) 532, 573, 640
BamHI GGATCC 1 cut(s) 465
BbrPI CACGTG 1 cut(s) 526
BbsI GAAGAC 2 cut(s) 721, 1040
Bbv12I GWGCWC 1 cut(s) 545
BbvI GCAGC 2 cut(s) 278, 470
BccI CCATC 2 cut(s) 805, 979
BceAI ACGGC 4 cut(s) 149, 316, 367, 514
BcgI CGANNNNNNTGC 2 cut(s) 187, 221
BciT130I CCWGG 3 cut(s) 389, 401, 537
BcnI CCSGG 2 cut(s) 412, 413
BcoDI GTCTC 3 cut(s) 316, 578, 1027
BfaI CTAG 1 cut(s) 969
BfmI CTRYAG 1 cut(s) 904
BfuAI ACCTGC 1 cut(s) 122
BglII AGATCT 1 cut(s) 937
BisI GCNGC 4 cut(s) 75, 289, 292, 484
BlsI GCNGC 4 cut(s) 76, 290, 293, 485
Bme1390I CCNGG 5 cut(s) 389, 401, 412, 413, 537
Bme18I GGWCC 3 cut(s) 532, 573, 640
BmeT110I CYCGRG 2 cut(s) 411, 626
BmgT120I GGNCC 3 cut(s) 532, 573, 640
BmiI GGNNCC 5 cut(s) 211, 304, 467, 534, 574
BmrFI CCNGG 5 cut(s) 389, 401, 412, 413, 537
BmrI ACTGGG 1 cut(s) 322
BmsI GCATC 4 cut(s) 597, 677, 816, 981
BmuI ACTGGG 1 cut(s) 322
BpiI GAAGAC 2 cut(s) 721, 1040
BpmI CTGGAG 1 cut(s) 1021
Bpu14I TTCGAA 1 cut(s) 812
BpuMI CCSGG 2 cut(s) 412, 413
BsaAI YACGTR 2 cut(s) 396, 526
BsaBI GATNNNNATC 1 cut(s) 843
BsaHI GRCGYC 1 cut(s) 309
BsaJI CCNNGG 4 cut(s) 411, 535, 536, 561
Bsc4I CCNNNNNNNGG 1 cut(s) 909
Bse118I RCCGGY 1 cut(s) 344
Bse1I ACTGG 2 cut(s) 328, 782
Bse3DI GCAATG 1 cut(s) 836
Bse8I GATNNNNATC 1 cut(s) 843
BseBI CCWGG 3 cut(s) 389, 401, 537
BseDI CCNNGG 4 cut(s) 411, 535, 536, 561
BseGI GGATG 1 cut(s) 612
BseJI GATNNNNATC 1 cut(s) 843
BseLI CCNNNNNNNGG 1 cut(s) 909
BseMI GCAATG 1 cut(s) 836
BseMII CTCAG 1 cut(s) 962
BseNI ACTGG 2 cut(s) 328, 782
BseRI GAGGAG 5 cut(s) 17, 32, 58, 578, 868
BseXI GCAGC 2 cut(s) 278, 470
BsgI GTGCAG 2 cut(s) 73, 569
Bsh1236I CGCG 1 cut(s) 385
BshFI GGCC 2 cut(s) 344, 354
BsiHKAI GWGCWC 1 cut(s) 545
BsiHKCI CYCGRG 2 cut(s) 411, 626
BsiSI CCGG 3 cut(s) 306, 345, 412
BslFI GGGAC 1 cut(s) 586
BslI CCNNNNNNNGG 1 cut(s) 909
BsmAI GTCTC 3 cut(s) 316, 578, 1027
BsmBI CGTCTC 3 cut(s) 316, 578, 1027
BsmFI GGGAC 1 cut(s) 586
BsnI GGCC 2 cut(s) 344, 354
BsoBI CYCGRG 2 cut(s) 411, 626
Bsp119I TTCGAA 1 cut(s) 812
Bsp1286I GDGCHC 1 cut(s) 545
Bsp143I GATC 6 cut(s) 405, 441, 465, 555, 597, 937
BspACI CCGC 8 cut(s) 60, 72, 75, 102, 196, 255, 289, 322
BspANI GGCC 2 cut(s) 344, 354
BspCNI CTCAG 1 cut(s) 961
BspFNI CGCG 1 cut(s) 385
BspLI GGNNCC 5 cut(s) 211, 304, 467, 534, 574
BspMI ACCTGC 1 cut(s) 122
BspPI GGATC 4 cut(s) 460, 473, 563, 605
BspQI GCTCTTC 1 cut(s) 1044
BspT104I TTCGAA 1 cut(s) 812
BsrDI GCAATG 1 cut(s) 836
BsrFI RCCGGY 1 cut(s) 344
BsrI ACTGG 2 cut(s) 328, 782
BssAI RCCGGY 1 cut(s) 344
BssECI CCNNGG 4 cut(s) 411, 535, 536, 561
BssMI GATC 6 cut(s) 405, 441, 465, 555, 597, 937
BssNI GRCGYC 1 cut(s) 309
Bst2UI CCWGG 3 cut(s) 389, 401, 537
Bst4CI ACNGT 1 cut(s) 547
Bst6I CTCTTC 2 cut(s) 182, 1044
BstACI GRCGYC 1 cut(s) 309
BstBAI YACGTR 2 cut(s) 396, 526
BstBI TTCGAA 1 cut(s) 812
BstC8I GCNNGC 2 cut(s) 346, 794
BstDEI CTNAG 1 cut(s) 948
BstF5I GGATG 1 cut(s) 612
BstFNI CGCG 1 cut(s) 385
BstHHI GCGC 1 cut(s) 387
BstKTI GATC 6 cut(s) 408, 444, 468, 558, 600, 940
BstMAI GTCTC 3 cut(s) 316, 578, 1027
BstMBI GATC 6 cut(s) 405, 441, 465, 555, 597, 937
BstMWI GCNNNNNNNGC 1 cut(s) 300
BstNI CCWGG 3 cut(s) 389, 401, 537
BstSCI CCNGG 5 cut(s) 387, 399, 410, 411, 535
BstSFI CTRYAG 1 cut(s) 904
BstUI CGCG 1 cut(s) 385
BstV1I GCAGC 2 cut(s) 278, 470
BstV2I GAAGAC 2 cut(s) 721, 1040
BstX2I RGATCY 2 cut(s) 465, 937
BstYI RGATCY 2 cut(s) 465, 937
BsuRI GGCC 2 cut(s) 344, 354
BtgZI GCGATG 1 cut(s) 958
BtsCI GGATG 1 cut(s) 612
BtsI GCAGTG 2 cut(s) 470, 732
BtsIMutI CAGTG 2 cut(s) 470, 732
BveI ACCTGC 1 cut(s) 122
Cac8I GCNNGC 2 cut(s) 346, 794
CaiI CAGNNNCTG 1 cut(s) 460
CfoI GCGC 1 cut(s) 387
Cfr10I RCCGGY 1 cut(s) 344
Cfr13I GGNCC 3 cut(s) 532, 573, 640
Cfr9I CCCGGG 1 cut(s) 411
CseI GACGC 2 cut(s) 298, 595
Csp6I GTAC 4 cut(s) 331, 397, 677, 913
CviQI GTAC 4 cut(s) 331, 397, 677, 913
DdeI CTNAG 1 cut(s) 948
DpnI GATC 6 cut(s) 407, 443, 467, 557, 599, 939
DpnII GATC 6 cut(s) 405, 441, 465, 555, 597, 937
DraIII CACNNNGTG 1 cut(s) 529
Eam1104I CTCTTC 2 cut(s) 182, 1044
EarI CTCTTC 2 cut(s) 182, 1044
EciI GGCGGA 2 cut(s) 61, 91
Eco47I GGWCC 3 cut(s) 532, 573, 640
Eco72I CACGTG 1 cut(s) 526
Eco88I CYCGRG 2 cut(s) 411, 626
EcoO109I RGGNCCY 1 cut(s) 640
EcoRII CCWGG 3 cut(s) 387, 399, 535
Esp3I CGTCTC 3 cut(s) 316, 578, 1027
FaiI YATR 8 cut(s) 143, 365, 425, 570, 726, 853, 906, 1060
FalI AAGNNNNNCTT 2 cut(s) 161, 193
FaqI GGGAC 1 cut(s) 586
Fnu4HI GCNGC 4 cut(s) 75, 289, 292, 484
FokI GGATG 1 cut(s) 619
Fsp4HI GCNGC 4 cut(s) 75, 289, 292, 484
FspBI CTAG 1 cut(s) 969
GlaI GCGC 1 cut(s) 386
GluI GCNGC 4 cut(s) 75, 289, 292, 484
GsuI CTGGAG 1 cut(s) 1021
HaeIII GGCC 2 cut(s) 344, 354
HapII CCGG 3 cut(s) 306, 345, 412
HgaI GACGC 2 cut(s) 298, 595
HhaI GCGC 1 cut(s) 387
Hin1I GRCGYC 1 cut(s) 309
Hin6I GCGC 1 cut(s) 385
HinP1I GCGC 1 cut(s) 385
HindIII AAGCTT 1 cut(s) 660
HinfI GANTC 4 cut(s) 273, 686, 695, 839
HpaII CCGG 3 cut(s) 306, 345, 412
HphI GGTGA 5 cut(s) 10, 22, 49, 178, 244
Hpy166II GTNNAC 3 cut(s) 496, 532, 954
Hpy188I TCNGA 3 cut(s) 209, 272, 597
Hpy188III TCNNGA 2 cut(s) 169, 229
Hpy8I GTNNAC 3 cut(s) 496, 532, 954
Hpy99I CGWCG 1 cut(s) 353
HpyAV CCTTC 4 cut(s) 91, 289, 602, 831
HpyCH4III ACNGT 1 cut(s) 547
HpyCH4IV ACGT 2 cut(s) 395, 525
HpyCH4V TGCA 6 cut(s) 90, 514, 550, 668, 829, 885
HpyF10VI GCNNNNNNNGC 1 cut(s) 300
HpyF3I CTNAG 1 cut(s) 948
HpySE526I ACGT 2 cut(s) 395, 525
Hsp92I GRCGYC 1 cut(s) 309
HspAI GCGC 1 cut(s) 385
KroI GCCGGC 1 cut(s) 344
KroNI GCCGGC 1 cut(s) 346
Kzo9I GATC 6 cut(s) 405, 441, 465, 555, 597, 937
LguI GCTCTTC 1 cut(s) 1044
LmnI GCTCC 7 cut(s) 30, 209, 273, 299, 308, 540, 594
Lsp1109I GCAGC 2 cut(s) 278, 470
LweI GCATC 4 cut(s) 597, 677, 816, 981
MaeI CTAG 1 cut(s) 969
MaeII ACGT 2 cut(s) 395, 525
MaeIII GTNAC 3 cut(s) 250, 391, 702
MalI GATC 6 cut(s) 407, 443, 467, 557, 599, 939
MboI GATC 6 cut(s) 405, 441, 465, 555, 597, 937
MboII GAAGA 6 cut(s) 169, 721, 864, 1031, 1040, 1046
MfeI CAATTG 1 cut(s) 742
MflI RGATCY 2 cut(s) 465, 937
MhlI GDGCHC 1 cut(s) 545
MluCI AATT 6 cut(s) 106, 742, 782, 808, 848, 1026
MlyI GAGTC 1 cut(s) 680
MmeI TCCRAC 2 cut(s) 321, 986
MroNI GCCGGC 1 cut(s) 344
MseI TTAA 1 cut(s) 807
MspA1I CMGCKG 2 cut(s) 291, 999
MspI CCGG 3 cut(s) 306, 345, 412
MspR9I CCNGG 5 cut(s) 389, 401, 412, 413, 537
MunI CAATTG 1 cut(s) 742
MvaI CCWGG 3 cut(s) 389, 401, 537
MvnI CGCG 1 cut(s) 385
MwoI GCNNNNNNNGC 1 cut(s) 300
NaeI GCCGGC 1 cut(s) 346
NciI CCSGG 2 cut(s) 412, 413
NdeII GATC 6 cut(s) 405, 441, 465, 555, 597, 937
NgoMIV GCCGGC 1 cut(s) 344
NlaIV GGNNCC 5 cut(s) 211, 304, 467, 534, 574
NmeAIII GCCGAG 1 cut(s) 586
NmuCI GTSAC 2 cut(s) 250, 391
NspV TTCGAA 1 cut(s) 812
PaeR7I CTCGAG 1 cut(s) 626
PaqCI CACCTGC 1 cut(s) 122
PasI CCCWGGG 1 cut(s) 536
PciSI GCTCTTC 1 cut(s) 1044
PcsI WCGNNNNNNNCGW 1 cut(s) 306
PdiI GCCGGC 1 cut(s) 346
PfeI GAWTC 3 cut(s) 273, 695, 839
PkrI GCNGC 4 cut(s) 76, 290, 293, 485
PleI GAGTC 1 cut(s) 680
PmaCI CACGTG 1 cut(s) 526
PmlI CACGTG 1 cut(s) 526
PpsI GAGTC 1 cut(s) 680
Ppu21I YACGTR 2 cut(s) 396, 526
PpuMI RGGWCCY 1 cut(s) 640
Psp5II RGGWCCY 1 cut(s) 640
Psp6I CCWGG 3 cut(s) 387, 399, 535
PspCI CACGTG 1 cut(s) 526
PspGI CCWGG 3 cut(s) 387, 399, 535
PspN4I GGNNCC 5 cut(s) 211, 304, 467, 534, 574
PspPI GGNCC 3 cut(s) 532, 573, 640
PspPPI RGGWCCY 1 cut(s) 640
PspXI VCTCGAGB 1 cut(s) 626
PstNI CAGNNNCTG 1 cut(s) 460
PsuI RGATCY 2 cut(s) 465, 937
PvuII CAGCTG 1 cut(s) 999
RsaI GTAC 4 cut(s) 332, 398, 678, 914
RsaNI GTAC 4 cut(s) 331, 397, 677, 913
SapI GCTCTTC 1 cut(s) 1044
SaqAI TTAA 1 cut(s) 807
SatI GCNGC 4 cut(s) 75, 289, 292, 484
Sau3AI GATC 6 cut(s) 405, 441, 465, 555, 597, 937
Sau96I GGNCC 3 cut(s) 532, 573, 640
SchI GAGTC 1 cut(s) 680
ScrFI CCNGG 5 cut(s) 389, 401, 412, 413, 537
SduI GDGCHC 1 cut(s) 545
SfaNI GCATC 4 cut(s) 597, 677, 816, 981
SfcI CTRYAG 1 cut(s) 904
Sfr274I CTCGAG 1 cut(s) 626
SfuI TTCGAA 1 cut(s) 812
SinI GGWCC 3 cut(s) 532, 573, 640
SlaI CTCGAG 1 cut(s) 626
SmaI CCCGGG 1 cut(s) 413
SmlI CTYRAG 1 cut(s) 626
SmoI CTYRAG 1 cut(s) 626
Sse9I AATT 6 cut(s) 106, 742, 782, 808, 848, 1026
SsiI CCGC 8 cut(s) 60, 72, 75, 102, 196, 255, 289, 322
SspMI CTAG 1 cut(s) 969
StyD4I CCNGG 5 cut(s) 387, 399, 410, 411, 535
TaaI ACNGT 1 cut(s) 547
TaiI ACGT 2 cut(s) 398, 528
TaqI TCGA 8 cut(s) 129, 230, 357, 444, 627, 689, 812, 824
TasI AATT 6 cut(s) 106, 742, 782, 808, 848, 1026
TatI WGTACW 1 cut(s) 330
TauI GCSGC 2 cut(s) 77, 291
TfiI GAWTC 3 cut(s) 273, 695, 839
Tru1I TTAA 1 cut(s) 807
Tru9I TTAA 1 cut(s) 807
TscAI CASTG 2 cut(s) 477, 739
TseFI GTSAC 2 cut(s) 250, 391
TseI GCWGC 2 cut(s) 291, 483
Tsp45I GTSAC 2 cut(s) 250, 391
TspDTI ATGAA 2 cut(s) 636, 1040
TspGWI ACGGA 1 cut(s) 534
TspMI CCCGGG 1 cut(s) 411
TspRI CASTG 2 cut(s) 477, 739
VpaK11BI GGWCC 3 cut(s) 532, 573, 640
XhoI CTCGAG 1 cut(s) 626
XmaI CCCGGG 1 cut(s) 411
XspI CTAG 1 cut(s) 969
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.