Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0006347

Physical Location & Seq

Genomic Coordinates Reverse (-)

19610324 .. 19616851

Gene Structure 6528 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1093400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2343 bp

ATGGAGAAAATTTTCATCCTCTCTCTCCTCCTTCTTCCTCTCTTAGCCTCATGTGCAGAACCAAAAAAGGTCTATATAGTGTATTTTGGAGAACACAGGGGAGAGAAAGCACTGCATGAAATTGAGGACATCCATCACTCGTATTTGCTCTCTGTGAAAGAAACAGGAGAAGATGCCAGAGCTTCTTTGCTTTACAGTTACAAGCACAGCATCAATGGCTTTGCAGCAGTCCTCACTCAGGACGAAGCTTCCAAGCTATCTGAGTTGGAAGAAGTTGTGTCTGTGTGGGCTAGCCACCCAAAAAAGTACTCTATGCACACCACAAGGTCTTGGGAGTTTTCAGGCATACTAGAAGAGGAACAGAGGCACTGGATAGACAGCAAGATGGGAGGAGACTTCTTATCCAAAGCCAGATTTGGCAAGGACATCATAGTTGGGCTTCTTGATAGTGGTGTCTGGCCAGAATCAAAGAGCTTTAGGGATGAAGGAATGGGGCCCGTACCAAATTCATGGAAGGGAATCTGCCAATCAGGACCTGGTTTTAATTCATCTCACTGTAACAGGAAGCTGATAGGAGCTCGCTACTACCTCAAAGGTTACGAGCAATACTATGGCAATGTAGTAAATGCCTCGGAGGATTACCGATCACCGCGTGACATGGATGGCCACGGCACACACACAAGTTCAACCGTTGCAGGCCGAGTTGTTCCAAATGCCGCAGCCCTGGGTGGTTTTGCCCTAGGCACCGCCTCTGGTGGTGCCCCATTGGCGCGCCTTGCCATATATAAAGTGTGTTGGGCGATACCAGGCCAGTCCAAGGTGGGCGGCAACACATGCTTTGAGGAAGACATGCTTGCAGCCATTGATGATGCAATAGGTGATGGTGTTGATGTACTGAGCATATCAATTGGGCCAAGCCAACCTGTAAATTACACCAATGATGGCATGGCTATTGGAGCATTGCATGCCACAAAGAAGAACATTGTGGTGGCATGTAGTGCTGGGAACTCTGGCCCTGCCCCATCTACTCTTGTAAATTCAGCTCCATGGATCATCACAGTTGGTGCTAGTAGCATCGACCGGGCATTCATCGCGCCAGTTGTGCTTGGAAATGGGATCACAATTGAGGGAGAAACAGTGACACCAAGCAAGCTGGAAGAGAATAAGAAGTACCCTTTAGTATATGCTGCAGATGTTATTAACTCTGGTGTACCCAAAGATATGGCAGGACAATGCCTACCGGGCTCTCTTTCACCTGAGAAGGTGAAGGGAAAGATAGTTTTGTGCATGAGAGGAAGTGGGATGAGAGTTGTAAAAGGCATGGAAGTGAAAAGGGCGGGAGGAGTTGGATTTATATTAGGAAACAGTGCAGAAAATGGGGTTGAAATTGCATGCGATGCTCATGTTATTCCTGCCACTTCGGTGCTTTATAAAGATGCCAATAGAATTCTTCAATACATCAACTCTACCAAAATTCCAACGGCTACTATTATACCAGCAAGGACTGTGTTGAATAGAAAGCCAGCTCCTTTCATGGCTGCATTTACTAGTAGAGGCCCCAATATCATTGACCCTAATTTTCTCAAGCCGGATATCACAGCTCCAGGGCTGAATATATTGGCAGCGTGGACTGAGGCTGATCCGCCCACCAAGTTGTCGGGTATAGATCACCGAGTTGTTCTGTATAATATACACTCCGGAACTTCAATGTCTTGCCCTCATGTAGCAGCTGCAGCAGCACTCGTCAAAGCAATCCACCCTACTTGGAGTGGTGCTGCCATAAGATCTGCTATCATGACCACAGCTGGAATAAGGAACAACGTGGGTTTGCCATTGAATGATGAATCAGGAAGTGTTGCCACTCCCTTTGCATATGGGGCTGGCCACTTTCGTCCCACGAAGGCAGCAGACCCCGGCCTTGTCTATGATGCTTCGTACAAAGACTATCTTCAGTACATTTGCAGCATAGGAGAAGCCAAGGACTTCGACCCAACGTTCAAGTGTCCAAAATCACCACCGGCAGCAATCAACTTGAACTACCCTTCCATTGCAATCCCCAAACTTAAGGACGCAATGATCATCAAGAGGACAGTCACAAACGTCGGCAACAGCAAAAGCATTTACTTCTTTACCGCCAAACCGCCTTTGGGAATCTCCGTTAAGGCATCCCCGAGCATATTGTTCTTCGATCATGTCGGCCAGAAGAAGAGCTTCACCATGACAGTTAAAGCAAGAAGAGAGATGCTTAGTAAGCACAGCACAGATGATTACGTCTTTGGGTGGTACACATGGACTGATGGGTTCTATACTGTGAGGAGTCCTATTGCAGTGTCGTTAGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0016787 GO:0017171 GO:0019538 GO:0043170 GO:0044238 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8

Protein Analysis

781

Amino Acids

83.79

Weight (kDa)

8.57

Isoelectric Point (pI)

34.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	24 - 100	3.4e-17	Peptidase inhibitor I9
Peptidase_S8	PF00082	140 - 628	7.1e-41	Subtilase family
PA	PF02225	390 - 483	7.4e-12	PA domain
fn3_6	PF17766	677 - 777	8.8e-28	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006347)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G45650 AT5G45650
fragaria_vesca	FvH4_3g06570 FvH4_3g06570 FvH4_3g06570 FvH4_3g06570 FvH4_3g06570 FvH4_3g06570 FvH4_3g33200
malus_domestica	MD00G1093400.v1.1 MD10G1270100.v1.1
prunus_persica	Prupe.4G071500_v2.0.a1
pyrus_communis	pycom05g27040 pycom10g22620
rosa_chinensis	RchiOBHm_Chr5g0012581
rosa_laevigata	RLG00000031926
rosa_multiflora	Rmu_co8464651.1_g000001 Rmu_sc0002639.1_g000013 Rmu_sc0003577.1_g000010
rosa_roxburghii	Rroxscaffold_1G00064090
rosa_rugosa	Rorug05G0002700 Rorug05G0002700 Rorug05G0002700
rosa_samantha	Rh5AG096500 Rh5CG105600 Rh5DG092200
rosa_wichuraiana	Rw5G008450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1431
AccB1I	GGYRCC	2 cut(s)	743, 758
AccII	CGCG	3 cut(s)	652, 772, 1094
AccIII	TCCGGA	1 cut(s)	1697
AciI	CCGC	8 cut(s)	650, 717, 747, 825, 1337, 1643, 2133, 2141
AclI	AACGTT	1 cut(s)	1994
AclWI	GGATC	3 cut(s)	1058, 1124, 1634
AcoI	YGGCCR	4 cut(s)	458, 664, 1882, 2197
AcsI	RAATTY	5 cut(s)	9, 505, 1036, 1446, 1473
AcuI	CTGAAG	1 cut(s)	1934
AdeI	CACNNNGTG	1 cut(s)	653
AfaI	GTAC	8 cut(s)	308, 501, 894, 1172, 1212, 1937, 1955, 2285
AfiI	CCNNNNNNNGG	3 cut(s)	238, 817, 2147
AflII	CTTAAG	1 cut(s)	2063
AgsI	TTSAA	8 cut(s)	687, 1385, 1454, 1513, 1707, 1837, 1999, 2035
AhlI	ACTAGT	1 cut(s)	1547
AjnI	CCWGG	4 cut(s)	535, 723, 805, 1603
AleI	CACNNNNGTG	1 cut(s)	1421
AloI	GAACNNNNNNTCC	2 cut(s)	2027, 2059
Alw21I	GWGCWC	1 cut(s)	580
Alw26I	GTCTC	1 cut(s)	387
AlwI	GGATC	3 cut(s)	1058, 1124, 1634
AlwNI	CAGNNNCTG	1 cut(s)	536
Ama87I	CYCGRG	1 cut(s)	2170
Aor13HI	TCCGGA	1 cut(s)	1697
ApaI	GGGCCC	1 cut(s)	498
ApoI	RAATTY	5 cut(s)	9, 505, 1036, 1446, 1473
AscI	GGCGCGCC	1 cut(s)	770
Asp700I	GAANNNNTTC	2 cut(s)	11, 2210
AspA2I	CCTAGG	1 cut(s)	739
AspLEI	GCGC	3 cut(s)	772, 774, 1096
AspS9I	GGNCC	6 cut(s)	494, 495, 533, 911, 1013, 1556
AsuC2I	CCSGG	3 cut(s)	1082, 1242, 1914
AsuHPI	GGTGA	7 cut(s)	639, 890, 1245, 1276, 1661, 2004, 2206
AsuNHI	GCTAGC	1 cut(s)	290
AvaI	CYCGRG	1 cut(s)	2170
AvaII	GGWCC	1 cut(s)	533
AvrII	CCTAGG	1 cut(s)	739
BaeGI	GKGCMC	2 cut(s)	498, 763
BalI	TGGCCA	3 cut(s)	460, 666, 1884
BanI	GGYRCC	2 cut(s)	743, 758
BanII	GRGCYC	3 cut(s)	498, 580, 1247
BbsI	GAAGAC	1 cut(s)	852
Bbv12I	GWGCWC	1 cut(s)	580
BccI	CCATC	7 cut(s)	141, 379, 656, 875, 935, 1030, 2291
BceAI	ACGGC	2 cut(s)	685, 1497
BciT130I	CCWGG	4 cut(s)	537, 725, 807, 1605
BclI	TGATCA	1 cut(s)	2076
BcnI	CCSGG	3 cut(s)	1082, 1242, 1914
BcoDI	GTCTC	1 cut(s)	387
BcuI	ACTAGT	1 cut(s)	1547
BfaI	CTAG	5 cut(s)	291, 350, 740, 1068, 1548
BfmI	CTRYAG	2 cut(s)	1188, 1731
BfrI	CTTAAG	1 cut(s)	2063
BglI	GCCNNNNNGGC	2 cut(s)	767, 1242
BglII	AGATCT	1 cut(s)	1784
BlnI	CCTAGG	1 cut(s)	739
BmcAI	AGTACT	1 cut(s)	308
Bme1390I	CCNGG	7 cut(s)	537, 725, 807, 1082, 1242, 1605, 1914
Bme18I	GGWCC	1 cut(s)	533
BmeT110I	CYCGRG	1 cut(s)	2170
BmgT120I	GGNCC	6 cut(s)	494, 495, 533, 911, 1013, 1556
BmiI	GGNNCC	5 cut(s)	495, 496, 745, 760, 1558
BmrFI	CCNGG	7 cut(s)	537, 725, 807, 1082, 1242, 1605, 1914
BmsI	GCATC	9 cut(s)	163, 219, 859, 1083, 1387, 1426, 1918, 2174, 2232
BmtI	GCTAGC	1 cut(s)	294
BpiI	GAAGAC	1 cut(s)	852
BpmI	CTGGAG	1 cut(s)	1587
BpuEI	CTTGAG	1 cut(s)	1568
BpuMI	CCSGG	3 cut(s)	1082, 1242, 1914
BsaWI	WCCGGW	1 cut(s)	1697
BsaXI	ACNNNNNCTCC	2 cut(s)	1122, 1152
Bsc4I	CCNNNNNNNGG	3 cut(s)	238, 817, 2147
Bse118I	RCCGGY	1 cut(s)	2017
Bse1I	ACTGG	3 cut(s)	374, 811, 1097
Bse3DI	GCAATG	4 cut(s)	622, 959, 2046, 2079
BseAI	TCCGGA	1 cut(s)	1697
BseBI	CCWGG	4 cut(s)	537, 725, 807, 1605
BseGI	GGATG	6 cut(s)	15, 129, 487, 667, 1308, 2165
BseLI	CCNNNNNNNGG	3 cut(s)	238, 817, 2147
BseMI	GCAATG	4 cut(s)	622, 959, 2046, 2079
BseMII	CTCAG	5 cut(s)	251, 252, 887, 1248, 1623
BseNI	ACTGG	3 cut(s)	374, 811, 1097
BsePI	GCGCGC	1 cut(s)	770
BseRI	GAGGAG	4 cut(s)	17, 405, 1356, 2329
BseSI	GKGCMC	2 cut(s)	498, 763
BseYI	CCCAGC	1 cut(s)	1001
BsgI	GTGCAG	2 cut(s)	75, 1389
Bsh1236I	CGCG	3 cut(s)	652, 772, 1094
Bsh1285I	CGRYCG	1 cut(s)	1081
BshNI	GGYRCC	2 cut(s)	743, 758
BsiEI	CGRYCG	1 cut(s)	1081
BsiHKAI	GWGCWC	1 cut(s)	580
BsiHKCI	CYCGRG	1 cut(s)	2170
BsiSI	CCGG	6 cut(s)	1081, 1241, 1589, 1698, 1914, 2018
BslFI	GGGAC	1 cut(s)	1878
BslI	CCNNNNNNNGG	3 cut(s)	238, 817, 2147
BsmAI	GTCTC	1 cut(s)	387
BsmFI	GGGAC	1 cut(s)	1878
BsmI	GAATGC	1 cut(s)	1085
BsoBI	CYCGRG	1 cut(s)	2170
Bsp120I	GGGCCC	1 cut(s)	494
Bsp1286I	GDGCHC	4 cut(s)	498, 580, 763, 1247
Bsp13I	TCCGGA	1 cut(s)	1697
Bsp143I	GATC	8 cut(s)	644, 1050, 1116, 1639, 1666, 1784, 2076, 2188
Bsp19I	CCATGG	1 cut(s)	1046
BspACI	CCGC	8 cut(s)	650, 717, 747, 825, 1337, 1643, 2133, 2141
BspCNI	CTCAG	5 cut(s)	250, 253, 888, 1249, 1624
BspEI	TCCGGA	1 cut(s)	1697
BspFNI	CGCG	3 cut(s)	652, 772, 1094
BspHI	TCATGA	1 cut(s)	1794
BspLI	GGNNCC	5 cut(s)	495, 496, 745, 760, 1558
BspMAI	CTGCAG	2 cut(s)	1192, 1735
BspOI	GCTAGC	1 cut(s)	294
BspPI	GGATC	3 cut(s)	1058, 1124, 1634
BspQI	GCTCTTC	1 cut(s)	2201
BspT107I	GGYRCC	2 cut(s)	743, 758
BspTI	CTTAAG	1 cut(s)	2063
BsrDI	GCAATG	4 cut(s)	622, 959, 2046, 2079
BsrFI	RCCGGY	1 cut(s)	2017
BsrI	ACTGG	3 cut(s)	374, 811, 1097
BssAI	RCCGGY	1 cut(s)	2017
BssHII	GCGCGC	1 cut(s)	770
BssMI	GATC	8 cut(s)	644, 1050, 1116, 1639, 1666, 1784, 2076, 2188
BssT1I	CCWWGG	4 cut(s)	739, 816, 1046, 1977
Bst2UI	CCWGG	4 cut(s)	537, 725, 807, 1605
Bst6I	CTCTTC	4 cut(s)	348, 1152, 2201, 2230
BstAFI	CTTAAG	1 cut(s)	2063
BstAPI	GCANNNNNTGC	4 cut(s)	834, 965, 998, 1397
BstDEI	CTNAG	7 cut(s)	43, 237, 261, 896, 1257, 1632, 2246
BstDSI	CCRYGG	2 cut(s)	667, 1046
BstENI	CCTNNNNNAGG	1 cut(s)	236
BstF5I	GGATG	6 cut(s)	15, 129, 487, 667, 1308, 2165
BstFNI	CGCG	3 cut(s)	652, 772, 1094
BstHHI	GCGC	3 cut(s)	772, 774, 1096
BstKTI	GATC	8 cut(s)	647, 1053, 1119, 1642, 1669, 1787, 2079, 2191
BstMAI	GTCTC	1 cut(s)	387
BstMBI	GATC	8 cut(s)	644, 1050, 1116, 1639, 1666, 1784, 2076, 2188
BstMCI	CGRYCG	1 cut(s)	1081
BstNI	CCWGG	4 cut(s)	537, 725, 807, 1605
BstNSI	RCATGY	5 cut(s)	837, 853, 968, 996, 1395
BstSCI	CCNGG	7 cut(s)	535, 723, 805, 1080, 1240, 1603, 1912
BstSFI	CTRYAG	2 cut(s)	1188, 1731
BstSLI	GKGCMC	2 cut(s)	498, 763
BstUI	CGCG	3 cut(s)	652, 772, 1094
BstV2I	GAAGAC	1 cut(s)	852
BstX2I	RGATCY	1 cut(s)	1784
BstXI	CCANNNNNNTGG	2 cut(s)	510, 1222
BstYI	RGATCY	1 cut(s)	1784
BtgI	CCRYGG	2 cut(s)	667, 1046
BtgZI	GCGATG	2 cut(s)	1075, 1410
BtsCI	GGATG	6 cut(s)	15, 129, 487, 667, 1308, 2165
BtsI	GCAGTG	2 cut(s)	110, 2334
BtsIMutI	CAGTG	6 cut(s)	110, 367, 553, 1143, 1372, 2334
CaiI	CAGNNNCTG	1 cut(s)	536
CciI	TCATGA	1 cut(s)	1794
CfoI	GCGC	3 cut(s)	772, 774, 1096
Cfr10I	RCCGGY	1 cut(s)	2017
Cfr13I	GGNCC	6 cut(s)	494, 495, 533, 911, 1013, 1556
CseI	GACGC	1 cut(s)	2078
CsiI	ACCWGGT	1 cut(s)	535
Csp6I	GTAC	8 cut(s)	307, 500, 893, 1171, 1211, 1936, 1954, 2284
CspCI	CAANNNNNGTGG	4 cut(s)	1745, 1780, 1849, 1884
CviQI	GTAC	8 cut(s)	307, 500, 893, 1171, 1211, 1936, 1954, 2284
DdeI	CTNAG	7 cut(s)	43, 237, 261, 896, 1257, 1632, 2246
DpnI	GATC	8 cut(s)	646, 1052, 1118, 1641, 1668, 1786, 2078, 2190
DpnII	GATC	8 cut(s)	644, 1050, 1116, 1639, 1666, 1784, 2076, 2188
DraIII	CACNNNGTG	1 cut(s)	653
EaeI	YGGCCR	4 cut(s)	458, 664, 1882, 2197
Eam1104I	CTCTTC	4 cut(s)	348, 1152, 2201, 2230
EarI	CTCTTC	4 cut(s)	348, 1152, 2201, 2230
EciI	GGCGGA	1 cut(s)	1632
Ecl136II	GAGCTC	1 cut(s)	578
Eco130I	CCWWGG	4 cut(s)	739, 816, 1046, 1977
Eco24I	GRGCYC	3 cut(s)	498, 580, 1247
Eco32I	GATATC	1 cut(s)	1594
Eco47I	GGWCC	1 cut(s)	533
Eco53kI	GAGCTC	1 cut(s)	578
Eco57I	CTGAAG	1 cut(s)	1934
Eco88I	CYCGRG	1 cut(s)	2170
EcoICRI	GAGCTC	1 cut(s)	578
EcoNI	CCTNNNNNAGG	1 cut(s)	236
EcoO109I	RGGNCCY	3 cut(s)	494, 533, 1556
EcoRI	GAATTC	1 cut(s)	1446
EcoRII	CCWGG	4 cut(s)	535, 723, 805, 1603
EcoRV	GATATC	1 cut(s)	1594
EcoT14I	CCWWGG	4 cut(s)	739, 816, 1046, 1977
EcoT38I	GRGCYC	3 cut(s)	498, 580, 1247
ErhI	CCWWGG	4 cut(s)	739, 816, 1046, 1977
FalI	AAGNNNNNCTT	6 cut(s)	837, 869, 1932, 1964, 2195, 2227
FaqI	GGGAC	1 cut(s)	1878
FauI	CCCGC	1 cut(s)	1330
FauNDI	CATATG	1 cut(s)	1873
FbaI	TGATCA	1 cut(s)	2076
FokI	GGATG	6 cut(s)	2, 116, 494, 674, 1315, 2152
FriOI	GRGCYC	3 cut(s)	498, 580, 1247
FspBI	CTAG	5 cut(s)	291, 350, 740, 1068, 1548
GlaI	GCGC	3 cut(s)	771, 773, 1095
GsaI	CCCAGC	1 cut(s)	1005
GsuI	CTGGAG	1 cut(s)	1587
HapII	CCGG	6 cut(s)	1081, 1241, 1589, 1698, 1914, 2018
HgaI	GACGC	1 cut(s)	2078
HhaI	GCGC	3 cut(s)	772, 774, 1096
Hin6I	GCGC	3 cut(s)	770, 772, 1094
HinP1I	GCGC	3 cut(s)	770, 772, 1094
HindIII	AAGCTT	1 cut(s)	246
HinfI	GANTC	5 cut(s)	464, 519, 1844, 2151, 2317
HpaII	CCGG	6 cut(s)	1081, 1241, 1589, 1698, 1914, 2018
HphI	GGTGA	7 cut(s)	639, 890, 1245, 1276, 1661, 2004, 2206
Hpy166II	GTNNAC	3 cut(s)	1211, 1629, 2286
Hpy188I	TCNGA	2 cut(s)	262, 634
Hpy188III	TCNNGA	7 cut(s)	239, 443, 531, 1698, 1795, 1848, 2083
Hpy8I	GTNNAC	3 cut(s)	1211, 1629, 2286
Hpy99I	CGWCG	1 cut(s)	2105
HpyAV	CCTTC	7 cut(s)	41, 479, 508, 1255, 1261, 1894, 2052
HpyCH4IV	ACGT	4 cut(s)	1821, 1994, 2100, 2271
HpyF3I	CTNAG	7 cut(s)	43, 237, 261, 896, 1257, 1632, 2246
HpySE526I	ACGT	4 cut(s)	1821, 1994, 2100, 2271
HspAI	GCGC	3 cut(s)	770, 772, 1094
Kpn2I	TCCGGA	1 cut(s)	1697
Ksp22I	TGATCA	1 cut(s)	2076
Kzo9I	GATC	8 cut(s)	644, 1050, 1116, 1639, 1666, 1784, 2076, 2188
LguI	GCTCTTC	1 cut(s)	2201
LmnI	GCTCC	5 cut(s)	575, 956, 1048, 1531, 1606
LweI	GCATC	9 cut(s)	163, 219, 859, 1083, 1387, 1426, 1918, 2174, 2232
MabI	ACCWGGT	1 cut(s)	535
MaeI	CTAG	5 cut(s)	291, 350, 740, 1068, 1548
MaeII	ACGT	4 cut(s)	1821, 1994, 2100, 2271
MaeIII	GTNAC	6 cut(s)	197, 557, 596, 653, 1138, 2092
MalI	GATC	8 cut(s)	646, 1052, 1118, 1641, 1668, 1786, 2078, 2190
MboI	GATC	8 cut(s)	644, 1050, 1116, 1639, 1666, 1784, 2076, 2188
MfeI	CAATTG	2 cut(s)	906, 1122
MflI	RGATCY	1 cut(s)	1784
MhlI	GDGCHC	4 cut(s)	498, 580, 763, 1247
MlsI	TGGCCA	3 cut(s)	460, 666, 1884
MluNI	TGGCCA	3 cut(s)	460, 666, 1884
MlyI	GAGTC	1 cut(s)	2326
MmeI	TCCRAC	3 cut(s)	246, 1327, 1502
Mox20I	TGGCCA	3 cut(s)	460, 666, 1884
MroI	TCCGGA	1 cut(s)	1697
MroXI	GAANNNNTTC	2 cut(s)	11, 2210
MscI	TGGCCA	3 cut(s)	460, 666, 1884
MseI	TTAA	5 cut(s)	543, 1200, 2064, 2160, 2226
MslI	CAYNNNNRTG	3 cut(s)	986, 1325, 1421
Msp20I	TGGCCA	3 cut(s)	460, 666, 1884
MspA1I	CMGCKG	2 cut(s)	1730, 1805
MspCI	CTTAAG	1 cut(s)	2063
MspI	CCGG	6 cut(s)	1081, 1241, 1589, 1698, 1914, 2018
MspR9I	CCNGG	7 cut(s)	537, 725, 807, 1082, 1242, 1605, 1914
MunI	CAATTG	2 cut(s)	906, 1122
Mva1269I	GAATGC	1 cut(s)	1085
MvaI	CCWGG	4 cut(s)	537, 725, 807, 1605
MvnI	CGCG	3 cut(s)	652, 772, 1094
NciI	CCSGG	3 cut(s)	1082, 1242, 1914
NcoI	CCATGG	1 cut(s)	1046
NdeI	CATATG	1 cut(s)	1873
NdeII	GATC	8 cut(s)	644, 1050, 1116, 1639, 1666, 1784, 2076, 2188
NheI	GCTAGC	1 cut(s)	290
NlaIV	GGNNCC	5 cut(s)	495, 496, 745, 760, 1558
NmeAIII	GCCGAG	1 cut(s)	725
NmuCI	GTSAC	3 cut(s)	653, 1138, 2092
NspI	RCATGY	5 cut(s)	837, 853, 968, 996, 1395
OliI	CACNNNNGTG	1 cut(s)	1421
PaeI	GCATGC	2 cut(s)	968, 1395
PagI	TCATGA	1 cut(s)	1794
PalAI	GGCGCGCC	1 cut(s)	770
PasI	CCCWGGG	1 cut(s)	724
PauI	GCGCGC	1 cut(s)	770
PciSI	GCTCTTC	1 cut(s)	2201
PctI	GAATGC	1 cut(s)	1085
PdmI	GAANNNNTTC	2 cut(s)	11, 2210
PfeI	GAWTC	4 cut(s)	464, 519, 1844, 2151
PleI	GAGTC	1 cut(s)	2325
PpsI	GAGTC	1 cut(s)	2325
PpuMI	RGGWCCY	1 cut(s)	533
PsiI	TTATAA	1 cut(s)	1431
Psp124BI	GAGCTC	1 cut(s)	580
Psp1406I	AACGTT	1 cut(s)	1994
Psp5II	RGGWCCY	1 cut(s)	533
Psp6I	CCWGG	4 cut(s)	535, 723, 805, 1603
PspFI	CCCAGC	1 cut(s)	1001
PspGI	CCWGG	4 cut(s)	535, 723, 805, 1603
PspN4I	GGNNCC	5 cut(s)	495, 496, 745, 760, 1558
PspOMI	GGGCCC	1 cut(s)	494
PspPI	GGNCC	6 cut(s)	494, 495, 533, 911, 1013, 1556
PspPPI	RGGWCCY	1 cut(s)	533
PstI	CTGCAG	2 cut(s)	1192, 1735
PstNI	CAGNNNCTG	1 cut(s)	536
PsuI	RGATCY	1 cut(s)	1784
PteI	GCGCGC	1 cut(s)	770
PvuII	CAGCTG	2 cut(s)	1730, 1805
RsaI	GTAC	8 cut(s)	308, 501, 894, 1172, 1212, 1937, 1955, 2285
RsaNI	GTAC	8 cut(s)	307, 500, 893, 1171, 1211, 1936, 1954, 2284
RseI	CAYNNNNRTG	3 cut(s)	986, 1325, 1421
SacI	GAGCTC	1 cut(s)	580
SapI	GCTCTTC	1 cut(s)	2201
SaqAI	TTAA	5 cut(s)	543, 1200, 2064, 2160, 2226
Sau3AI	GATC	8 cut(s)	644, 1050, 1116, 1639, 1666, 1784, 2076, 2188
Sau96I	GGNCC	6 cut(s)	494, 495, 533, 911, 1013, 1556
ScaI	AGTACT	1 cut(s)	308
SchI	GAGTC	1 cut(s)	2326
ScrFI	CCNGG	7 cut(s)	537, 725, 807, 1082, 1242, 1605, 1914
SduI	GDGCHC	4 cut(s)	498, 580, 763, 1247
SexAI	ACCWGGT	1 cut(s)	535
SfaNI	GCATC	9 cut(s)	163, 219, 859, 1083, 1387, 1426, 1918, 2174, 2232
SfcI	CTRYAG	2 cut(s)	1188, 1731
SgsI	GGCGCGCC	1 cut(s)	770
SinI	GGWCC	1 cut(s)	533
SmiMI	CAYNNNNRTG	3 cut(s)	986, 1325, 1421
SmlI	CTYRAG	2 cut(s)	1583, 2063
SmoI	CTYRAG	2 cut(s)	1583, 2063
SpeI	ACTAGT	1 cut(s)	1547
SphI	GCATGC	2 cut(s)	968, 1395
SsiI	CCGC	8 cut(s)	650, 717, 747, 825, 1337, 1643, 2133, 2141
SspMI	CTAG	5 cut(s)	291, 350, 740, 1068, 1548
SstI	GAGCTC	1 cut(s)	580
StyD4I	CCNGG	7 cut(s)	535, 723, 805, 1080, 1240, 1603, 1912
StyI	CCWWGG	4 cut(s)	739, 816, 1046, 1977
TaiI	ACGT	4 cut(s)	1824, 1997, 2103, 2274
TaqI	TCGA	3 cut(s)	1077, 1986, 2187
TatI	WGTACW	3 cut(s)	306, 892, 1953
TauI	GCSGC	2 cut(s)	719, 828
TfiI	GAWTC	4 cut(s)	464, 519, 1844, 2151
Tru1I	TTAA	5 cut(s)	543, 1200, 2064, 2160, 2226
Tru9I	TTAA	5 cut(s)	543, 1200, 2064, 2160, 2226
TscAI	CASTG	6 cut(s)	117, 374, 560, 1143, 1372, 2334
TseFI	GTSAC	3 cut(s)	653, 1138, 2092
Tsp45I	GTSAC	3 cut(s)	653, 1138, 2092
TspDTI	ATGAA	8 cut(s)	4, 132, 498, 498, 537, 1078, 1522, 1857
TspGWI	ACGGA	1 cut(s)	2146
TspRI	CASTG	6 cut(s)	117, 374, 560, 1143, 1372, 2334
Vha464I	CTTAAG	1 cut(s)	2063
VpaK11BI	GGWCC	1 cut(s)	533
XagI	CCTNNNNNAGG	1 cut(s)	236
XapI	RAATTY	5 cut(s)	9, 505, 1036, 1446, 1473
XceI	RCATGY	5 cut(s)	837, 853, 968, 996, 1395
XcmI	CCANNNNNNNNNTGG	3 cut(s)	533, 943, 2143
XmaJI	CCTAGG	1 cut(s)	739
XmnI	GAANNNNTTC	2 cut(s)	11, 2210
XspI	CTAG	5 cut(s)	291, 350, 740, 1068, 1548
ZrmI	AGTACT	1 cut(s)	308

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.