Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0006347

Physical Location & Seq

Genomic Coordinates Reverse (-)

28110971 .. 28116580

Gene Structure 5610 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom05g27040.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2346 bp

ATGGAGAAAATTTTCATCCTCTCTCTCCTTCTCCTTCTTCCTCTCTTAGCCTCATGTGCAGATCAAAAAAAGGTCTACATAGTGTATTTTGGAGAACACAGGGGAGAGAACGCACTCCATGAAATTGAGGACATCCATCACTCGTATTTGCTCTCTGTGAAAGAAACAGAAGAAGATGCCAGAGCTTCTTTGCTTTACAGTTACAAGCACAGCATCAATGGCTTTGCAGCAGTCCTCACTCAGGACGAAGCTTCCAAACTATCTGGGTTGGAAGAAGTTGTGTCTGTGTGGGCTAGCCACCCAAAAAAGTACTCTATGCACACCACAAGGTCTTGGGAGTTTTCAGGCATACTAGAAGAGGAACAGAGGCACTGGATGGACAACAAGATGGGAGGAGACTTCTTATCCAAAGCCAGATTTGGCAAGGACATCATAGTTGGGCTCCTTGATAGTGGTGTCTGGCCAGAATCAAATAGCTTTAGGGATGAAGGAATGGGGCCCGTACCAAATTCATGGAAGGGAATCTGCCAATCAGGACCTGGTTTTAATTCATCTCACTGTAACAGGAAGCTGATAGGAGCTCGCTACTACCTCAAAGGTTACGAGCAATACTATGGCCATGTAGTAAATGCCTCGGAGGATTACCGATCACCGCGTGACATGGATGGCCACGGCACACACACAAGTTCAACCGTTGCAGGCCGAGTCGTTCCAAATGCCGCAGCCCTGGGTGGTTTTGCCCTAGGCACCGCCTCTGGTGGTGCCCCATTGGCGCGCCTTGCCATATATAAAGTGTGTTGGGCGATACCAGGCCATTCCAAGGTGGGCGGCAACACATGCTTTGAGGAAGACATGCTTGCAGCCATTGATGATGCAATAGGTGATGGTGTTGATGTACTGAGCATATCAGTTGGGCTAAGCCAACCTGTAAATTACACCAATGATGGCATTGCTATTGGAGCATTGCATGCCACAAAGAAGAACATTGTGGTGGCATGTAGTGCTGGGAACTCTGGCCCTGCCCCATCTACTCTTGTAAATTCAGCTCCATGGATCATCACAGTTGGTGCTAGTAGCCTCGACCGGGCATTCATCGCACCAGTTGTGCTTGGAAATGGGATCACAATTGAGGGAGAAACAGTGACACCAAGCAAGCTGGAAGAGAATAAGAAGTACCCTTTAGTATATGCTGCAGATGTTATTAACTCTGGTGTACCCAAAGATATTGCAGGACAATGCCTACCAGGCTCTCTTTCACCTGAGAAGGTGAATGGAAAGATAGTTTTGTGCATGAGAGGAAGTGGGATGAGAGTTGTAAAAGGCATGGAAGTGAAAAGGGCAGGAGGAGTTGGATTTATATTAGGAAACAGTGCAGAAAATGGGGTTGAAATTGCATGCGATGCTCATGTTATTCCTGCCACTTCGGTGCTTTATAAAGATGCCAATAGAATTCTTCAATACATCAACTCTACCAAAATTCCAACGGCTACTATTATACCTGCAAGGACTGTGTTGAATAGAAAGCCAGCTCCTTTCATGGCTGCATTTACTAGTAGAGGCCCCAATATCATTGACCCTAATTTTCTCAAGCCGGATATCACAGCTCCAGGGCTGAATATATTGGCAGCGTGGACTGAGGCTGATCCGCCAACCAAGTTGTCGGGTATAGATCGCCGAGTTGTTCAGTATAATATATACTCCGGAACTTCAATGTCTTGCCCTCATGTAGCAGCTGCAGCAGCACTCGTCAAAGCAATCCACCCTACTTGGAGCGGTGCTGCCATAAGATCTGCTATCATGACCACAGCTGGAATAAGGAACAACTTGGGTTTGCCATTGAATGATGAATCAGGAAGTGTTGCTACTCCCTTTGCATATGGGGCTGGCCACTTTCGTCCCACGAAGGCAGCAGACCCCGGCCTTGTCTATGATGCTTCATACAAAGACTATCTTCAGTACATTTGCAGCATAGGAGGAGCCAAGGACTTCGACCCAACGTTCAAGTGTCCAACATCACCGCCGGCAGCAATCAACTTGAACTACCCTTCCATTGCAATCCCCAAACTTAAGGACGCAATGATCATCAAGAGGACAGTCACAAACGTCGGCAACAGCAAAAGCATTTACTTCTTTACAGCCAAACCACCTTTAGGAGTCTCCATTAAGGCATCCCCGAGCATATTGTTCTTCGATCATGTCGGGCAGAAGAAGAGCTTCACCATAACAGTTAAAGCAAGAAGAGAGGTGCTTAGTAAGCACAGCAAAGATGAGTACGTCTTTGGGTGGTACACATGGACTGATGGGTTGTATACTGTGAGGAGTCCTATTGCAGTGTCGTTAGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0016787 GO:0017171 GO:0019538 GO:0043170 GO:0044238 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8

Protein Analysis

782

Amino Acids

83.85

Weight (kDa)

8.48

Isoelectric Point (pI)

34.58

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006347)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G45650 AT5G45650
fragaria_vesca	FvH4_3g06570 FvH4_3g06570 FvH4_3g06570 FvH4_3g06570 FvH4_3g06570 FvH4_3g06570 FvH4_3g33200
malus_domestica	MD00G1093400.v1.1 MD10G1270100.v1.1
prunus_persica	Prupe.4G071500_v2.0.a1
pyrus_communis	pycom05g27040 pycom10g22620
rosa_chinensis	RchiOBHm_Chr5g0012581
rosa_laevigata	RLG00000031926
rosa_multiflora	Rmu_co8464651.1_g000001 Rmu_sc0002639.1_g000013 Rmu_sc0003577.1_g000010
rosa_roxburghii	Rroxscaffold_1G00064090
rosa_rugosa	Rorug05G0002700 Rorug05G0002700 Rorug05G0002700
rosa_samantha	Rh5AG096500 Rh5CG105600 Rh5DG092200
rosa_wichuraiana	Rw5G008450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1434
Acc36I	ACCTGC	1 cut(s)	1507
AccB1I	GGYRCC	2 cut(s)	746, 761
AccBSI	CCGCTC	1 cut(s)	1773
AccI	GTMKAC	2 cut(s)	75, 2309
AccII	CGCG	2 cut(s)	655, 775
AccIII	TCCGGA	1 cut(s)	1700
AciI	CCGC	7 cut(s)	653, 720, 750, 828, 1646, 1773, 2018
AclI	AACGTT	1 cut(s)	1997
AclWI	GGATC	3 cut(s)	1061, 1127, 1637
AcoI	YGGCCR	4 cut(s)	461, 616, 667, 1885
AcsI	RAATTY	5 cut(s)	9, 508, 1039, 1449, 1476
AcuI	CTGAAG	1 cut(s)	1937
AdeI	CACNNNGTG	1 cut(s)	656
AfaI	GTAC	8 cut(s)	311, 504, 897, 1175, 1215, 1958, 2273, 2288
AfiI	CCNNNNNNNGG	4 cut(s)	241, 820, 1084, 2150
AflII	CTTAAG	1 cut(s)	2066
AgsI	TTSAA	8 cut(s)	690, 1388, 1457, 1516, 1710, 1840, 2002, 2038
AhlI	ACTAGT	1 cut(s)	1550
AjnI	CCWGG	5 cut(s)	538, 726, 808, 1243, 1606
AleI	CACNNNNGTG	1 cut(s)	1424
AloI	GAACNNNNNNTCC	2 cut(s)	2030, 2062
Alw21I	GWGCWC	1 cut(s)	583
Alw26I	GTCTC	2 cut(s)	390, 2161
AlwI	GGATC	3 cut(s)	1061, 1127, 1637
AlwNI	CAGNNNCTG	1 cut(s)	539
Ama87I	CYCGRG	1 cut(s)	2173
Aor13HI	TCCGGA	1 cut(s)	1700
ApaI	GGGCCC	1 cut(s)	501
ApoI	RAATTY	5 cut(s)	9, 508, 1039, 1449, 1476
AscI	GGCGCGCC	1 cut(s)	773
Asp700I	GAANNNNTTC	2 cut(s)	11, 2213
AspA2I	CCTAGG	1 cut(s)	742
AspLEI	GCGC	2 cut(s)	775, 777
AspS9I	GGNCC	5 cut(s)	497, 498, 536, 1016, 1559
AsuC2I	CCSGG	2 cut(s)	1085, 1917
AsuHPI	GGTGA	6 cut(s)	642, 893, 1248, 1279, 2007, 2209
AsuNHI	GCTAGC	1 cut(s)	293
AvaI	CYCGRG	1 cut(s)	2173
AvaII	GGWCC	1 cut(s)	536
AvrII	CCTAGG	1 cut(s)	742
BaeGI	GKGCMC	2 cut(s)	501, 766
BalI	TGGCCA	4 cut(s)	463, 618, 669, 1887
BanI	GGYRCC	2 cut(s)	746, 761
BanII	GRGCYC	3 cut(s)	444, 501, 583
BarI	GAAGNNNNNNTAC	2 cut(s)	1846, 1878
BbsI	GAAGAC	1 cut(s)	855
Bbv12I	GWGCWC	1 cut(s)	583
BccI	CCATC	8 cut(s)	144, 370, 382, 659, 878, 938, 1033, 2294
BceAI	ACGGC	2 cut(s)	688, 1500
BciT130I	CCWGG	5 cut(s)	540, 728, 810, 1245, 1608
BclI	TGATCA	1 cut(s)	2079
BcnI	CCSGG	2 cut(s)	1085, 1917
BcoDI	GTCTC	2 cut(s)	390, 2161
BcuI	ACTAGT	1 cut(s)	1550
BfaI	CTAG	5 cut(s)	294, 353, 743, 1071, 1551
BfmI	CTRYAG	2 cut(s)	1191, 1734
BfrI	CTTAAG	1 cut(s)	2066
BfuAI	ACCTGC	1 cut(s)	1507
BglI	GCCNNNNNGGC	2 cut(s)	770, 1245
BglII	AGATCT	1 cut(s)	1787
BlnI	CCTAGG	1 cut(s)	742
BlpI	GCTNAGC	1 cut(s)	917
BmcAI	AGTACT	1 cut(s)	311
Bme1390I	CCNGG	7 cut(s)	540, 728, 810, 1085, 1245, 1608, 1917
Bme18I	GGWCC	1 cut(s)	536
BmeT110I	CYCGRG	1 cut(s)	2173
BmgT120I	GGNCC	5 cut(s)	497, 498, 536, 1016, 1559
BmiI	GGNNCC	7 cut(s)	443, 498, 499, 748, 763, 1561, 1978
BmrFI	CCNGG	7 cut(s)	540, 728, 810, 1085, 1245, 1608, 1917
BmsI	GCATC	7 cut(s)	166, 222, 862, 1390, 1429, 1921, 2177
BmtI	GCTAGC	1 cut(s)	297
BpiI	GAAGAC	1 cut(s)	855
BpmI	CTGGAG	1 cut(s)	1590
Bpu1102I	GCTNAGC	1 cut(s)	917
BpuEI	CTTGAG	1 cut(s)	1571
BpuMI	CCSGG	2 cut(s)	1085, 1917
BsaWI	WCCGGW	1 cut(s)	1700
BsaXI	ACNNNNNCTCC	2 cut(s)	1125, 1155
Bsc4I	CCNNNNNNNGG	4 cut(s)	241, 820, 1084, 2150
Bse118I	RCCGGY	1 cut(s)	2020
Bse1I	ACTGG	2 cut(s)	377, 1100
Bse3DI	GCAATG	4 cut(s)	948, 962, 2049, 2082
BseAI	TCCGGA	1 cut(s)	1700
BseBI	CCWGG	5 cut(s)	540, 728, 810, 1245, 1608
BseGI	GGATG	7 cut(s)	15, 132, 381, 490, 670, 1311, 2168
BseLI	CCNNNNNNNGG	4 cut(s)	241, 820, 1084, 2150
BseMI	GCAATG	4 cut(s)	948, 962, 2049, 2082
BseMII	CTCAG	4 cut(s)	254, 890, 1251, 1626
BseNI	ACTGG	2 cut(s)	377, 1100
BsePI	GCGCGC	1 cut(s)	773
BseRI	GAGGAG	4 cut(s)	408, 1359, 1989, 2332
BseSI	GKGCMC	2 cut(s)	501, 766
BseYI	CCCAGC	1 cut(s)	1004
BsgI	GTGCAG	2 cut(s)	78, 1392
Bsh1236I	CGCG	2 cut(s)	655, 775
Bsh1285I	CGRYCG	1 cut(s)	1084
BshNI	GGYRCC	2 cut(s)	746, 761
BsiEI	CGRYCG	1 cut(s)	1084
BsiHKAI	GWGCWC	1 cut(s)	583
BsiHKCI	CYCGRG	1 cut(s)	2173
BsiSI	CCGG	5 cut(s)	1084, 1592, 1701, 1917, 2021
BslFI	GGGAC	1 cut(s)	1881
BslI	CCNNNNNNNGG	4 cut(s)	241, 820, 1084, 2150
BsmAI	GTCTC	2 cut(s)	390, 2161
BsmFI	GGGAC	1 cut(s)	1881
BsmI	GAATGC	1 cut(s)	1088
BsoBI	CYCGRG	1 cut(s)	2173
Bsp120I	GGGCCC	1 cut(s)	497
Bsp1286I	GDGCHC	4 cut(s)	444, 501, 583, 766
Bsp13I	TCCGGA	1 cut(s)	1700
Bsp143I	GATC	9 cut(s)	61, 647, 1053, 1119, 1642, 1669, 1787, 2079, 2191
Bsp1720I	GCTNAGC	1 cut(s)	917
Bsp19I	CCATGG	1 cut(s)	1049
BspACI	CCGC	7 cut(s)	653, 720, 750, 828, 1646, 1773, 2018
BspCNI	CTCAG	4 cut(s)	253, 891, 1252, 1627
BspEI	TCCGGA	1 cut(s)	1700
BspFNI	CGCG	2 cut(s)	655, 775
BspHI	TCATGA	1 cut(s)	1797
BspLI	GGNNCC	7 cut(s)	443, 498, 499, 748, 763, 1561, 1978
BspMAI	CTGCAG	2 cut(s)	1195, 1738
BspMI	ACCTGC	1 cut(s)	1507
BspOI	GCTAGC	1 cut(s)	297
BspPI	GGATC	3 cut(s)	1061, 1127, 1637
BspQI	GCTCTTC	1 cut(s)	2204
BspT107I	GGYRCC	2 cut(s)	746, 761
BspTI	CTTAAG	1 cut(s)	2066
BsrBI	CCGCTC	1 cut(s)	1773
BsrDI	GCAATG	4 cut(s)	948, 962, 2049, 2082
BsrFI	RCCGGY	1 cut(s)	2020
BsrI	ACTGG	2 cut(s)	377, 1100
BssAI	RCCGGY	1 cut(s)	2020
BssHII	GCGCGC	1 cut(s)	773
BssMI	GATC	9 cut(s)	61, 647, 1053, 1119, 1642, 1669, 1787, 2079, 2191
BssNAI	GTATAC	1 cut(s)	2310
BssT1I	CCWWGG	4 cut(s)	742, 819, 1049, 1980
Bst1107I	GTATAC	1 cut(s)	2310
Bst2UI	CCWGG	5 cut(s)	540, 728, 810, 1245, 1608
Bst6I	CTCTTC	4 cut(s)	351, 1155, 2204, 2233
BstAFI	CTTAAG	1 cut(s)	2066
BstAPI	GCANNNNNTGC	4 cut(s)	837, 968, 1001, 1400
BstDEI	CTNAG	7 cut(s)	46, 240, 899, 917, 1260, 1635, 2249
BstDSI	CCRYGG	2 cut(s)	670, 1049
BstENI	CCTNNNNNAGG	1 cut(s)	239
BstF5I	GGATG	7 cut(s)	15, 132, 381, 490, 670, 1311, 2168
BstFNI	CGCG	2 cut(s)	655, 775
BstHHI	GCGC	2 cut(s)	775, 777
BstKTI	GATC	9 cut(s)	64, 650, 1056, 1122, 1645, 1672, 1790, 2082, 2194
BstMAI	GTCTC	2 cut(s)	390, 2161
BstMBI	GATC	9 cut(s)	61, 647, 1053, 1119, 1642, 1669, 1787, 2079, 2191
BstMCI	CGRYCG	1 cut(s)	1084
BstNI	CCWGG	5 cut(s)	540, 728, 810, 1245, 1608
BstNSI	RCATGY	5 cut(s)	840, 856, 971, 999, 1398
BstSCI	CCNGG	7 cut(s)	538, 726, 808, 1083, 1243, 1606, 1915
BstSFI	CTRYAG	2 cut(s)	1191, 1734
BstSLI	GKGCMC	2 cut(s)	501, 766
BstUI	CGCG	2 cut(s)	655, 775
BstV2I	GAAGAC	1 cut(s)	855
BstX2I	RGATCY	1 cut(s)	1787
BstXI	CCANNNNNNTGG	1 cut(s)	513
BstYI	RGATCY	1 cut(s)	1787
BstZ17I	GTATAC	1 cut(s)	2310
BtgI	CCRYGG	2 cut(s)	670, 1049
BtgZI	GCGATG	2 cut(s)	1078, 1413
BtsCI	GGATG	7 cut(s)	15, 132, 381, 490, 670, 1311, 2168
BtsI	GCAGTG	1 cut(s)	2337
BtsIMutI	CAGTG	5 cut(s)	370, 556, 1146, 1375, 2337
BveI	ACCTGC	1 cut(s)	1507
CaiI	CAGNNNCTG	1 cut(s)	539
CciI	TCATGA	1 cut(s)	1797
CfoI	GCGC	2 cut(s)	775, 777
Cfr10I	RCCGGY	1 cut(s)	2020
Cfr13I	GGNCC	5 cut(s)	497, 498, 536, 1016, 1559
CseI	GACGC	1 cut(s)	2081
CsiI	ACCWGGT	1 cut(s)	538
Csp6I	GTAC	8 cut(s)	310, 503, 896, 1174, 1214, 1957, 2272, 2287
CspCI	CAANNNNNGTGG	2 cut(s)	1748, 1783
CviQI	GTAC	8 cut(s)	310, 503, 896, 1174, 1214, 1957, 2272, 2287
DdeI	CTNAG	7 cut(s)	46, 240, 899, 917, 1260, 1635, 2249
DpnI	GATC	9 cut(s)	63, 649, 1055, 1121, 1644, 1671, 1789, 2081, 2193
DpnII	GATC	9 cut(s)	61, 647, 1053, 1119, 1642, 1669, 1787, 2079, 2191
DraIII	CACNNNGTG	1 cut(s)	656
EaeI	YGGCCR	4 cut(s)	461, 616, 667, 1885
Eam1104I	CTCTTC	4 cut(s)	351, 1155, 2204, 2233
EarI	CTCTTC	4 cut(s)	351, 1155, 2204, 2233
EciI	GGCGGA	1 cut(s)	1635
Ecl136II	GAGCTC	1 cut(s)	581
Eco130I	CCWWGG	4 cut(s)	742, 819, 1049, 1980
Eco24I	GRGCYC	3 cut(s)	444, 501, 583
Eco32I	GATATC	1 cut(s)	1597
Eco47I	GGWCC	1 cut(s)	536
Eco53kI	GAGCTC	1 cut(s)	581
Eco57I	CTGAAG	1 cut(s)	1937
Eco88I	CYCGRG	1 cut(s)	2173
EcoICRI	GAGCTC	1 cut(s)	581
EcoNI	CCTNNNNNAGG	1 cut(s)	239
EcoO109I	RGGNCCY	3 cut(s)	497, 536, 1559
EcoRI	GAATTC	1 cut(s)	1449
EcoRII	CCWGG	5 cut(s)	538, 726, 808, 1243, 1606
EcoRV	GATATC	1 cut(s)	1597
EcoT14I	CCWWGG	4 cut(s)	742, 819, 1049, 1980
EcoT38I	GRGCYC	3 cut(s)	444, 501, 583
ErhI	CCWWGG	4 cut(s)	742, 819, 1049, 1980
FalI	AAGNNNNNCTT	6 cut(s)	840, 872, 1935, 1967, 2198, 2230
FaqI	GGGAC	1 cut(s)	1881
FauNDI	CATATG	1 cut(s)	1876
FbaI	TGATCA	1 cut(s)	2079
FblI	GTMKAC	2 cut(s)	75, 2309
FokI	GGATG	7 cut(s)	2, 119, 388, 497, 677, 1318, 2155
FriOI	GRGCYC	3 cut(s)	444, 501, 583
FspBI	CTAG	5 cut(s)	294, 353, 743, 1071, 1551
GlaI	GCGC	2 cut(s)	774, 776
GsaI	CCCAGC	1 cut(s)	1008
GsuI	CTGGAG	1 cut(s)	1590
HapII	CCGG	5 cut(s)	1084, 1592, 1701, 1917, 2021
HgaI	GACGC	1 cut(s)	2081
HhaI	GCGC	2 cut(s)	775, 777
Hin6I	GCGC	2 cut(s)	773, 775
HinP1I	GCGC	2 cut(s)	773, 775
HindIII	AAGCTT	1 cut(s)	249
HinfI	GANTC	6 cut(s)	467, 522, 705, 1847, 2154, 2320
HpaII	CCGG	5 cut(s)	1084, 1592, 1701, 1917, 2021
HphI	GGTGA	6 cut(s)	642, 893, 1248, 1279, 2007, 2209
Hpy166II	GTNNAC	5 cut(s)	76, 1214, 1632, 2289, 2310
Hpy188I	TCNGA	1 cut(s)	637
Hpy188III	TCNNGA	6 cut(s)	242, 534, 1701, 1798, 1851, 2086
Hpy8I	GTNNAC	5 cut(s)	76, 1214, 1632, 2289, 2310
Hpy99I	CGWCG	1 cut(s)	2108
HpyAV	CCTTC	7 cut(s)	38, 44, 482, 511, 1258, 1897, 2055
HpyCH4IV	ACGT	3 cut(s)	1997, 2103, 2274
HpyF3I	CTNAG	7 cut(s)	46, 240, 899, 917, 1260, 1635, 2249
HpySE526I	ACGT	3 cut(s)	1997, 2103, 2274
HspAI	GCGC	2 cut(s)	773, 775
Kpn2I	TCCGGA	1 cut(s)	1700
KroI	GCCGGC	1 cut(s)	2020
KroNI	GCCGGC	1 cut(s)	2022
Ksp22I	TGATCA	1 cut(s)	2079
Kzo9I	GATC	9 cut(s)	61, 647, 1053, 1119, 1642, 1669, 1787, 2079, 2191
LguI	GCTCTTC	1 cut(s)	2204
LmnI	GCTCC	8 cut(s)	447, 578, 959, 1051, 1534, 1609, 1770, 1976
LweI	GCATC	7 cut(s)	166, 222, 862, 1390, 1429, 1921, 2177
MabI	ACCWGGT	1 cut(s)	538
MaeI	CTAG	5 cut(s)	294, 353, 743, 1071, 1551
MaeII	ACGT	3 cut(s)	1997, 2103, 2274
MaeIII	GTNAC	6 cut(s)	200, 560, 599, 656, 1141, 2095
MalI	GATC	9 cut(s)	63, 649, 1055, 1121, 1644, 1671, 1789, 2081, 2193
MbiI	CCGCTC	1 cut(s)	1773
MboI	GATC	9 cut(s)	61, 647, 1053, 1119, 1642, 1669, 1787, 2079, 2191
MfeI	CAATTG	1 cut(s)	1125
MflI	RGATCY	1 cut(s)	1787
MhlI	GDGCHC	4 cut(s)	444, 501, 583, 766
MlsI	TGGCCA	4 cut(s)	463, 618, 669, 1887
MluNI	TGGCCA	4 cut(s)	463, 618, 669, 1887
MlyI	GAGTC	3 cut(s)	714, 2163, 2329
MmeI	TCCRAC	4 cut(s)	249, 1330, 1505, 2033
Mox20I	TGGCCA	4 cut(s)	463, 618, 669, 1887
MroI	TCCGGA	1 cut(s)	1700
MroNI	GCCGGC	1 cut(s)	2020
MroXI	GAANNNNTTC	2 cut(s)	11, 2213
MscI	TGGCCA	4 cut(s)	463, 618, 669, 1887
MseI	TTAA	5 cut(s)	546, 1203, 2067, 2163, 2229
MslI	CAYNNNNRTG	3 cut(s)	989, 1328, 1424
Msp20I	TGGCCA	4 cut(s)	463, 618, 669, 1887
MspA1I	CMGCKG	2 cut(s)	1733, 1808
MspCI	CTTAAG	1 cut(s)	2066
MspI	CCGG	5 cut(s)	1084, 1592, 1701, 1917, 2021
MspR9I	CCNGG	7 cut(s)	540, 728, 810, 1085, 1245, 1608, 1917
MunI	CAATTG	1 cut(s)	1125
Mva1269I	GAATGC	1 cut(s)	1088
MvaI	CCWGG	5 cut(s)	540, 728, 810, 1245, 1608
MvnI	CGCG	2 cut(s)	655, 775
NaeI	GCCGGC	1 cut(s)	2022
NciI	CCSGG	2 cut(s)	1085, 1917
NcoI	CCATGG	1 cut(s)	1049
NdeI	CATATG	1 cut(s)	1876
NdeII	GATC	9 cut(s)	61, 647, 1053, 1119, 1642, 1669, 1787, 2079, 2191
NgoMIV	GCCGGC	1 cut(s)	2020
NheI	GCTAGC	1 cut(s)	293
NlaIV	GGNNCC	7 cut(s)	443, 498, 499, 748, 763, 1561, 1978
NmeAIII	GCCGAG	2 cut(s)	728, 1700
NmuCI	GTSAC	3 cut(s)	656, 1141, 2095
NspI	RCATGY	5 cut(s)	840, 856, 971, 999, 1398
OliI	CACNNNNGTG	1 cut(s)	1424
PaeI	GCATGC	2 cut(s)	971, 1398
PagI	TCATGA	1 cut(s)	1797
PalAI	GGCGCGCC	1 cut(s)	773
PasI	CCCWGGG	1 cut(s)	727
PauI	GCGCGC	1 cut(s)	773
PciSI	GCTCTTC	1 cut(s)	2204
PctI	GAATGC	1 cut(s)	1088
PdiI	GCCGGC	1 cut(s)	2022
PdmI	GAANNNNTTC	2 cut(s)	11, 2213
PfeI	GAWTC	3 cut(s)	467, 522, 1847
PleI	GAGTC	3 cut(s)	713, 2162, 2328
PpsI	GAGTC	3 cut(s)	713, 2162, 2328
PpuMI	RGGWCCY	1 cut(s)	536
PsiI	TTATAA	1 cut(s)	1434
Psp124BI	GAGCTC	1 cut(s)	583
Psp1406I	AACGTT	1 cut(s)	1997
Psp5II	RGGWCCY	1 cut(s)	536
Psp6I	CCWGG	5 cut(s)	538, 726, 808, 1243, 1606
PspFI	CCCAGC	1 cut(s)	1004
PspGI	CCWGG	5 cut(s)	538, 726, 808, 1243, 1606
PspN4I	GGNNCC	7 cut(s)	443, 498, 499, 748, 763, 1561, 1978
PspOMI	GGGCCC	1 cut(s)	497
PspPI	GGNCC	5 cut(s)	497, 498, 536, 1016, 1559
PspPPI	RGGWCCY	1 cut(s)	536
PstI	CTGCAG	2 cut(s)	1195, 1738
PstNI	CAGNNNCTG	1 cut(s)	539
PsuI	RGATCY	1 cut(s)	1787
PteI	GCGCGC	1 cut(s)	773
PvuII	CAGCTG	2 cut(s)	1733, 1808
RsaI	GTAC	8 cut(s)	311, 504, 897, 1175, 1215, 1958, 2273, 2288
RsaNI	GTAC	8 cut(s)	310, 503, 896, 1174, 1214, 1957, 2272, 2287
RseI	CAYNNNNRTG	3 cut(s)	989, 1328, 1424
SacI	GAGCTC	1 cut(s)	583
SapI	GCTCTTC	1 cut(s)	2204
SaqAI	TTAA	5 cut(s)	546, 1203, 2067, 2163, 2229
Sau3AI	GATC	9 cut(s)	61, 647, 1053, 1119, 1642, 1669, 1787, 2079, 2191
Sau96I	GGNCC	5 cut(s)	497, 498, 536, 1016, 1559
ScaI	AGTACT	1 cut(s)	311
SchI	GAGTC	3 cut(s)	714, 2163, 2329
ScrFI	CCNGG	7 cut(s)	540, 728, 810, 1085, 1245, 1608, 1917
SduI	GDGCHC	4 cut(s)	444, 501, 583, 766
SexAI	ACCWGGT	1 cut(s)	538
SfaNI	GCATC	7 cut(s)	166, 222, 862, 1390, 1429, 1921, 2177
SfcI	CTRYAG	2 cut(s)	1191, 1734
SgsI	GGCGCGCC	1 cut(s)	773
SinI	GGWCC	1 cut(s)	536
SmiMI	CAYNNNNRTG	3 cut(s)	989, 1328, 1424
SmlI	CTYRAG	2 cut(s)	1586, 2066
SmoI	CTYRAG	2 cut(s)	1586, 2066
SpeI	ACTAGT	1 cut(s)	1550
SphI	GCATGC	2 cut(s)	971, 1398
SsiI	CCGC	7 cut(s)	653, 720, 750, 828, 1646, 1773, 2018
SspMI	CTAG	5 cut(s)	294, 353, 743, 1071, 1551
SstI	GAGCTC	1 cut(s)	583
StyD4I	CCNGG	7 cut(s)	538, 726, 808, 1083, 1243, 1606, 1915
StyI	CCWWGG	4 cut(s)	742, 819, 1049, 1980
TaiI	ACGT	3 cut(s)	2000, 2106, 2277
TaqI	TCGA	3 cut(s)	1080, 1989, 2190
TatI	WGTACW	3 cut(s)	309, 895, 1956
TauI	GCSGC	2 cut(s)	722, 831
TfiI	GAWTC	3 cut(s)	467, 522, 1847
Tru1I	TTAA	5 cut(s)	546, 1203, 2067, 2163, 2229
Tru9I	TTAA	5 cut(s)	546, 1203, 2067, 2163, 2229
TscAI	CASTG	5 cut(s)	377, 563, 1146, 1375, 2337
TseFI	GTSAC	3 cut(s)	656, 1141, 2095
Tsp45I	GTSAC	3 cut(s)	656, 1141, 2095
TspDTI	ATGAA	9 cut(s)	4, 135, 501, 501, 540, 1081, 1525, 1860, 1926
TspRI	CASTG	5 cut(s)	377, 563, 1146, 1375, 2337
Vha464I	CTTAAG	1 cut(s)	2066
VpaK11BI	GGWCC	1 cut(s)	536
XagI	CCTNNNNNAGG	1 cut(s)	239
XapI	RAATTY	5 cut(s)	9, 508, 1039, 1449, 1476
XceI	RCATGY	5 cut(s)	840, 856, 971, 999, 1398
XcmI	CCANNNNNNNNNTGG	1 cut(s)	536
XmaJI	CCTAGG	1 cut(s)	742
XmiI	GTMKAC	2 cut(s)	75, 2309
XmnI	GAANNNNTTC	2 cut(s)	11, 2213
XspI	CTAG	5 cut(s)	294, 353, 743, 1071, 1551
ZrmI	AGTACT	1 cut(s)	311

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.