Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0006347

Physical Location & Seq

Genomic Coordinates Forward (+)

9576494 .. 9582761

Gene Structure 6268 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000031926

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2343 bp

ATGAAGCCAGTTTTCTTCATCATCATATCTCTCCTTCTCCTTCCTCTCTTGGCCTCATGCTCAAAGCAAAAGGTGTACATAGTGTATTTTGGAGAGCACGGTGGAGAGAAAGCATTGCATGAAATAGAGAACAGCCACCACTCCTATTTGCTTTCTGTGAAAGAAACTGAGGAAGATGCCAGAGCTTCACTGCTTTACAGTTACAAGCACAGCATCAATGGCTTTGCAGCAGTTCTCACTGAGGATGAAGCTTCCAAGTTATCTGAACTGGAAGAAGTTGTATCAGTGTGGCCGAGCCACCCAAGAAAGTACACTATGCACACAACAAGGTCATGGGAGTTTGTGGGGATATCGGAAGAAGAGGAAGAGGGGAATTACTGGGAGAACAACCAGATGGGAGGAGACTTCTTATCCAAAGCAAGGTCTGGCAAGAACATCATTGTTGGAGTCCTTGATAGTGGTGTATGGCCAGAATCAAAGAGCTTCAGTGATGTAGGAATGGGGCCCATTCCAAAAACATGGAAGGGAATCTGCCAATCTGGAGTTGGCTTTAATTCGTCCCACTGCAATAGGAAGCTTATAGGAGCTCGGTACTACCTCAAAGGGTTTGAGCAAAACTACGGTCCTCTTAATGCCTCAGAGGATTCTCGATCGCCACGTGACATGGACGGCCATGGAACCCACACGACGTCCACTGTGGCAGGCCGAGTCGTTCCGAATGCCTCAGCCATTGGCGGGTTCGCTCGCGGCTCTGCCTCCGGGGGTGCGCCATTGGCACACGTTGCTGTGTACAAAGTGTGCTGGGCTGTACCGGGCGCATCCAAGGCTGAAGGGAACACATGCTTTGAGGAGGACATGTTTGCAGCCATAGATGATGCCATAGCGGACGGTGTGGATGTGCTGAGCATATCAATTGGCACAGCCCGACCTATGAATTTCACCGAGGACGGTATTGCTCTCGGAGCATTGCATGCCGCCAAGAAGAACATTGTGGTGGCATGTAGTGCTGGCAACTCAGGCCCTGCTCCATCTACATTATCAAACCCAGCTCCATGGATCATCACGGTTGGTGCCAGCAGCTTGGACCGAACTTTTGTGTCACCCGTTGTGCTTGGAAATGGTTTGAGCATTGAGGGAGAAACAGTGACCCCAAGCAAGCTGGAAGAGAATAAGATGTACCCCTTAGTATATGCAGGGGATGTTGTTAATCCTGGTGTAGCCCAAAATCTGACAGGACAATGCCTCGCAGGCTCTCTTTCACCTGACAAAGTTAAGGGAAAGATAGTGTTGTGCTTGAGAGGTGCTGGGATGAGAGTTTCAAAAGGCATGGAAGTGAAAAGGGCGGGTGGTGTTGGTTTTATCTTAGGAAACAGCAAAGCAAATGGCGTTGAAATCTCGTGTGATGCTCATGTTCTGCCTGCCACTGCAATGACATACAGTAATGCCAATAGAATCATGGAATACATCAACTCTACCAAAAATCCAAAGGCAACTATTATACCAGCAAGGACAGTGTTGAATACAAAGCCAGCTCCATACATGACTGCTTTCACTAGTAGAGGCCCAAGTACCATTGATCCTTACATTCTCAAGCCTGATATCACAGCTCCTGGACTGAATATATTGGCAGCATGGACTGAGGCCGATCCCCCTACCAAGTTGTCAATGGATCACCGAGTGGCTCAGTATAACATTGAGTCTGGAACTTCAATGTCTTGCCCTCACATTGCTGCGGCGGCAGCACTTCTTAAAGCCATCCACCCTACTTGGAGCAGTGCTGCCATCAAATCTGCTCTAATGACCACAGCGGGGATAAGAAACAACCTAGATAAGCTATTGAACGATGAATCGGGCAATGCTGCAACTCCCTTTGCATATGGTGCTGGCCACTTCCGACCCACCAAGGCAGCAGATCCTGGCCTTGTGTACGATGCTTCATACAAAGACTATCTTCTCTACTTCTGCAGTACCGGAGTCAAGAATTTCGATCCAAAATTCAAGTGTCCAAGATCACCACCAACAGCAATCAACCTGAACTATCCTTCCATTGCAATCCCCAAACTGAATGGCACCATCACGATTAAGAGGACAGTCACAAATGTTGGTGACCCCAAAAGCGTCTACTTCTTTACCTCCAAACCGCCTCTTGGAATCTCGGTTAAGGCCTCTCCGAGTATATTGTTCTTTGATCACGTTGGCCAGAGGAAGAGCTTCACCATTACTGTCAAAGCAAGGACAGAGATGCTTAACGAGAATCAGTTGAAAGATGAGTATGCATTTGGGTGGTACACATGGACTGATGGACCTCATATTGTAAGGAGTCCAATTGCAGTCTCTCTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0016787 GO:0017171 GO:0019538 GO:0043170 GO:0044238 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8

Protein Analysis

781

Amino Acids

83.68

Weight (kDa)

8.09

Isoelectric Point (pI)

34.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	25 - 103	2.8e-20	Peptidase inhibitor I9
Peptidase_S8	PF00082	143 - 629	3.5e-40	Subtilase family
PA	PF02225	394 - 485	2.9e-11	PA domain
fn3_6	PF17766	677 - 778	2.5e-29	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006347)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G45650 AT5G45650
fragaria_vesca	FvH4_3g06570 FvH4_3g06570 FvH4_3g06570 FvH4_3g06570 FvH4_3g06570 FvH4_3g06570 FvH4_3g33200
malus_domestica	MD00G1093400.v1.1 MD10G1270100.v1.1
prunus_persica	Prupe.4G071500_v2.0.a1
pyrus_communis	pycom05g27040 pycom10g22620
rosa_chinensis	RchiOBHm_Chr5g0012581
rosa_laevigata	RLG00000031926
rosa_multiflora	Rmu_co8464651.1_g000001 Rmu_sc0002639.1_g000013 Rmu_sc0003577.1_g000010
rosa_roxburghii	Rroxscaffold_1G00064090
rosa_rugosa	Rorug05G0002700 Rorug05G0002700 Rorug05G0002700
rosa_samantha	Rh5AG096500 Rh5CG105600 Rh5DG092200
rosa_wichuraiana	Rw5G008450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	692
AccB1I	GGYRCC	2 cut(s)	1070, 2069
AccI	GTMKAC	1 cut(s)	2121
AccII	CGCG	1 cut(s)	747
AciI	CCGC	9 cut(s)	735, 747, 884, 975, 1343, 1733, 1736, 1808, 2141
AclWI	GGATC	6 cut(s)	1064, 1571, 1640, 1677, 1907, 1982
AcoI	YGGCCR	5 cut(s)	290, 467, 670, 1885, 2197
AcsI	RAATTY	3 cut(s)	934, 1981, 1994
AcuI	CTGAAG	2 cut(s)	469, 849
AcvI	CACGTG	1 cut(s)	659
AcyI	GRCGYC	1 cut(s)	689
AdeI	CACNNNGTG	1 cut(s)	1678
AfiI	CCNNNNNNNGG	4 cut(s)	420, 735, 1809, 2147
AflIII	ACRYGT	2 cut(s)	778, 855
AgsI	TTSAA	7 cut(s)	1320, 1391, 1519, 1710, 1840, 1999, 2263
AhlI	ACTAGT	1 cut(s)	1553
AjnI	CCWGG	3 cut(s)	1210, 1609, 1915
AloI	GAACNNNNNNTCC	2 cut(s)	2027, 2059
Alw21I	GWGCWC	2 cut(s)	99, 589
Alw26I	GTCTC	2 cut(s)	396, 2338
AlwI	GGATC	6 cut(s)	1064, 1571, 1640, 1677, 1907, 1982
AlwNI	CAGNNNCTG	3 cut(s)	1022, 1610, 1916
ApaI	GGGCCC	1 cut(s)	507
ApeKI	GCWGC	9 cut(s)	227, 863, 1077, 1628, 1730, 1739, 1778, 1859, 1907
ApoI	RAATTY	3 cut(s)	934, 1981, 1994
ArsI	GACNNNNNNTTYG	4 cut(s)	607, 639, 1076, 1108
Asp700I	GAANNNNTTC	1 cut(s)	2210
AspLEI	GCGC	2 cut(s)	769, 818
AspS9I	GGNCC	7 cut(s)	503, 504, 623, 1019, 1084, 1562, 2303
AsuC2I	CCSGG	2 cut(s)	760, 813
AsuHPI	GGTGA	7 cut(s)	931, 1092, 1251, 1664, 2004, 2117, 2206
AvaII	GGWCC	3 cut(s)	623, 1084, 2303
BaeGI	GKGCMC	1 cut(s)	507
BalI	TGGCCA	3 cut(s)	469, 1887, 2199
BanI	GGYRCC	2 cut(s)	1070, 2069
BanII	GRGCYC	2 cut(s)	507, 589
BauI	CACGAG	1 cut(s)	1396
BbrPI	CACGTG	1 cut(s)	659
Bbv12I	GWGCWC	2 cut(s)	99, 589
BbvCI	CCTCAGC	1 cut(s)	724
BbvI	GCAGC	9 cut(s)	239, 875, 1089, 1640, 1717, 1751, 1765, 1846, 1919
BccI	CCATC	6 cut(s)	388, 1036, 1763, 1790, 2081, 2294
BceAI	ACGGC	1 cut(s)	685
BciT130I	CCWGG	3 cut(s)	1212, 1611, 1917
BclI	TGATCA	1 cut(s)	2188
BcnI	CCSGG	2 cut(s)	760, 813
BcoDI	GTCTC	2 cut(s)	396, 2338
BcuI	ACTAGT	1 cut(s)	1553
BfaI	CTAG	2 cut(s)	1554, 1826
BfmI	CTRYAG	1 cut(s)	1963
BglI	GCCNNNNNGGC	1 cut(s)	1248
BlpI	GCTNAGC	1 cut(s)	902
Bme1390I	CCNGG	5 cut(s)	760, 813, 1212, 1611, 1917
Bme18I	GGWCC	3 cut(s)	623, 1084, 2303
BmgT120I	GGNCC	7 cut(s)	503, 504, 623, 1019, 1084, 1562, 2303
BmiI	GGNNCC	5 cut(s)	504, 505, 679, 1072, 2071
BmrFI	CCNGG	5 cut(s)	760, 813, 1212, 1611, 1917
BmrI	ACTGGG	1 cut(s)	388
BmsI	GCATC	7 cut(s)	166, 222, 827, 865, 1393, 1921, 2232
BmuI	ACTGGG	1 cut(s)	388
BpmI	CTGGAG	1 cut(s)	561
Bpu10I	CCTNAGC	1 cut(s)	724
Bpu1102I	GCTNAGC	1 cut(s)	902
BpuEI	CTTGAG	2 cut(s)	1315, 1574
BpuMI	CCSGG	2 cut(s)	760, 813
BsaAI	YACGTR	1 cut(s)	659
BsaHI	GRCGYC	1 cut(s)	689
BsaJI	CCNNGG	6 cut(s)	673, 759, 822, 942, 1052, 1902
BsaWI	WCCGGW	1 cut(s)	1970
BsaXI	ACNNNNNCTCC	4 cut(s)	842, 872, 1128, 1158
Bsc4I	CCNNNNNNNGG	4 cut(s)	420, 735, 1809, 2147
Bse1I	ACTGG	3 cut(s)	8, 273, 383
Bse3DI	GCAATG	6 cut(s)	113, 965, 1434, 1725, 1861, 2046
BseBI	CCWGG	3 cut(s)	1212, 1611, 1917
BseDI	CCNNGG	6 cut(s)	673, 759, 822, 942, 1052, 1902
BseGI	GGATG	6 cut(s)	250, 818, 901, 1204, 1314, 1755
BseLI	CCNNNNNNNGG	4 cut(s)	420, 735, 1809, 2147
BseMI	GCAATG	6 cut(s)	113, 965, 1434, 1725, 1861, 2046
BseMII	CTCAG	8 cut(s)	159, 231, 651, 738, 893, 1029, 1629, 1697
BseNI	ACTGG	3 cut(s)	8, 273, 383
BseRI	GAGGAG	2 cut(s)	414, 863
BseSI	GKGCMC	1 cut(s)	507
BseXI	GCAGC	9 cut(s)	239, 875, 1089, 1640, 1717, 1751, 1765, 1846, 1919
BseYI	CCCAGC	3 cut(s)	801, 1045, 1304
Bsh1236I	CGCG	1 cut(s)	747
Bsh1285I	CGRYCG	1 cut(s)	653
BshNI	GGYRCC	2 cut(s)	1070, 2069
BsiEI	CGRYCG	1 cut(s)	653
BsiHKAI	GWGCWC	2 cut(s)	99, 589
BsiSI	CCGG	3 cut(s)	759, 812, 1971
BslFI	GGGAC	1 cut(s)	544
BslI	CCNNNNNNNGG	4 cut(s)	420, 735, 1809, 2147
BsmAI	GTCTC	2 cut(s)	396, 2338
BsmFI	GGGAC	1 cut(s)	544
BsmI	GAATGC	1 cut(s)	724
Bsp120I	GGGCCC	1 cut(s)	503
Bsp1286I	GDGCHC	3 cut(s)	99, 507, 589
Bsp1407I	TGTACA	2 cut(s)	75, 789
Bsp143I	GATC	9 cut(s)	650, 1056, 1576, 1645, 1669, 1912, 1987, 2009, 2188
Bsp1720I	GCTNAGC	1 cut(s)	902
Bsp19I	CCATGG	2 cut(s)	673, 1052
BspACI	CCGC	9 cut(s)	735, 747, 884, 975, 1343, 1733, 1736, 1808, 2141
BspCNI	CTCAG	8 cut(s)	160, 232, 650, 737, 894, 1028, 1630, 1696
BspFNI	CGCG	1 cut(s)	747
BspLI	GGNNCC	5 cut(s)	504, 505, 679, 1072, 2071
BspMAI	CTGCAG	1 cut(s)	1967
BspPI	GGATC	6 cut(s)	1064, 1571, 1640, 1677, 1907, 1982
BspQI	GCTCTTC	1 cut(s)	2201
BspT107I	GGYRCC	2 cut(s)	1070, 2069
BsrDI	GCAATG	6 cut(s)	113, 965, 1434, 1725, 1861, 2046
BsrGI	TGTACA	2 cut(s)	75, 789
BsrI	ACTGG	3 cut(s)	8, 273, 383
BssECI	CCNNGG	6 cut(s)	673, 759, 822, 942, 1052, 1902
BssMI	GATC	9 cut(s)	650, 1056, 1576, 1645, 1669, 1912, 1987, 2009, 2188
BssNI	GRCGYC	1 cut(s)	689
BssSI	CACGAG	1 cut(s)	1396
BssT1I	CCWWGG	4 cut(s)	673, 822, 1052, 1902
Bst2BI	CACGAG	1 cut(s)	1396
Bst2UI	CCWGG	3 cut(s)	1212, 1611, 1917
Bst6I	CTCTTC	4 cut(s)	354, 360, 1158, 2201
BstACI	GRCGYC	1 cut(s)	689
BstAPI	GCANNNNNTGC	4 cut(s)	782, 971, 1004, 1880
BstAUI	TGTACA	2 cut(s)	75, 789
BstBAI	YACGTR	1 cut(s)	659
BstDSI	CCRYGG	2 cut(s)	673, 1052
BstEII	GGTNACC	1 cut(s)	2105
BstF5I	GGATG	6 cut(s)	250, 818, 901, 1204, 1314, 1755
BstFNI	CGCG	1 cut(s)	747
BstHHI	GCGC	2 cut(s)	769, 818
BstKTI	GATC	9 cut(s)	653, 1059, 1579, 1648, 1672, 1915, 1990, 2012, 2191
BstMAI	GTCTC	2 cut(s)	396, 2338
BstMBI	GATC	9 cut(s)	650, 1056, 1576, 1645, 1669, 1912, 1987, 2009, 2188
BstMCI	CGRYCG	1 cut(s)	653
BstNI	CCWGG	3 cut(s)	1212, 1611, 1917
BstNSI	RCATGY	4 cut(s)	843, 859, 974, 1002
BstPI	GGTNACC	1 cut(s)	2105
BstSCI	CCNGG	5 cut(s)	758, 811, 1210, 1609, 1915
BstSFI	CTRYAG	1 cut(s)	1963
BstSLI	GKGCMC	1 cut(s)	507
BstUI	CGCG	1 cut(s)	747
BstV1I	GCAGC	9 cut(s)	239, 875, 1089, 1640, 1717, 1751, 1765, 1846, 1919
BstX2I	RGATCY	1 cut(s)	1912
BstXI	CCANNNNNNTGG	3 cut(s)	519, 1053, 1081
BstYI	RGATCY	1 cut(s)	1912
BtgI	CCRYGG	2 cut(s)	673, 1052
BtsCI	GGATG	6 cut(s)	250, 818, 901, 1204, 1314, 1755
BtsI	GCAGTG	4 cut(s)	188, 562, 1422, 1780
CaiI	CAGNNNCTG	3 cut(s)	1022, 1610, 1916
CfoI	GCGC	2 cut(s)	769, 818
Cfr13I	GGNCC	7 cut(s)	503, 504, 623, 1019, 1084, 1562, 2303
CseI	GACGC	1 cut(s)	2107
CspCI	CAANNNNNGTGG	2 cut(s)	1748, 1783
DpnI	GATC	9 cut(s)	652, 1058, 1578, 1647, 1671, 1914, 1989, 2011, 2190
DpnII	GATC	9 cut(s)	650, 1056, 1576, 1645, 1669, 1912, 1987, 2009, 2188
DraIII	CACNNNGTG	1 cut(s)	1678
EaeI	YGGCCR	5 cut(s)	290, 467, 670, 1885, 2197
Eam1104I	CTCTTC	4 cut(s)	354, 360, 1158, 2201
EarI	CTCTTC	4 cut(s)	354, 360, 1158, 2201
Ecl136II	GAGCTC	1 cut(s)	587
Eco130I	CCWWGG	4 cut(s)	673, 822, 1052, 1902
Eco147I	AGGCCT	1 cut(s)	2165
Eco24I	GRGCYC	2 cut(s)	507, 589
Eco32I	GATATC	2 cut(s)	351, 1600
Eco47I	GGWCC	3 cut(s)	623, 1084, 2303
Eco53kI	GAGCTC	1 cut(s)	587
Eco57I	CTGAAG	2 cut(s)	469, 849
Eco72I	CACGTG	1 cut(s)	659
Eco91I	GGTNACC	1 cut(s)	2105
EcoICRI	GAGCTC	1 cut(s)	587
EcoO109I	RGGNCCY	2 cut(s)	503, 1019
EcoO65I	GGTNACC	1 cut(s)	2105
EcoRII	CCWGG	3 cut(s)	1210, 1609, 1915
EcoRV	GATATC	2 cut(s)	351, 1600
EcoT14I	CCWWGG	4 cut(s)	673, 822, 1052, 1902
EcoT22I	ATGCAT	1 cut(s)	2278
EcoT38I	GRGCYC	2 cut(s)	507, 589
ErhI	CCWWGG	4 cut(s)	673, 822, 1052, 1902
FalI	AAGNNNNNCTT	2 cut(s)	1935, 1967
FaqI	GGGAC	1 cut(s)	544
FauI	CCCGC	3 cut(s)	728, 1336, 1801
FauNDI	CATATG	1 cut(s)	1876
FbaI	TGATCA	1 cut(s)	2188
FblI	GTMKAC	1 cut(s)	2121
FokI	GGATG	6 cut(s)	257, 805, 908, 1211, 1321, 1742
FriOI	GRGCYC	2 cut(s)	507, 589
FspBI	CTAG	2 cut(s)	1554, 1826
GlaI	GCGC	2 cut(s)	768, 817
GsaI	CCCAGC	3 cut(s)	805, 1049, 1308
GsuI	CTGGAG	1 cut(s)	561
HapII	CCGG	3 cut(s)	759, 812, 1971
HgaI	GACGC	1 cut(s)	2107
HhaI	GCGC	2 cut(s)	769, 818
Hin1I	GRCGYC	1 cut(s)	689
Hin6I	GCGC	2 cut(s)	767, 816
HinP1I	GCGC	2 cut(s)	767, 816
HindIII	AAGCTT	2 cut(s)	249, 575
HpaII	CCGG	3 cut(s)	759, 812, 1971
HphI	GGTGA	7 cut(s)	931, 1092, 1251, 1664, 2004, 2117, 2206
Hpy166II	GTNNAC	7 cut(s)	76, 312, 693, 790, 1927, 2122, 2289
Hpy188I	TCNGA	8 cut(s)	265, 355, 640, 717, 962, 1230, 1895, 2172
Hpy188III	TCNNGA	5 cut(s)	540, 648, 1701, 1978, 2077
Hpy8I	GTNNAC	7 cut(s)	76, 312, 693, 790, 1927, 2122, 2289
Hpy99I	CGWCG	1 cut(s)	691
HpyAV	CCTTC	5 cut(s)	44, 50, 517, 824, 2052
HpyCH4IV	ACGT	4 cut(s)	658, 689, 780, 2193
HpySE526I	ACGT	4 cut(s)	658, 689, 780, 2193
Hsp92I	GRCGYC	1 cut(s)	689
HspAI	GCGC	2 cut(s)	767, 816
Ksp22I	TGATCA	1 cut(s)	2188
Kzo9I	GATC	9 cut(s)	650, 1056, 1576, 1645, 1669, 1912, 1987, 2009, 2188
LguI	GCTCTTC	1 cut(s)	2201
LmnI	GCTCC	7 cut(s)	584, 962, 1030, 1054, 1537, 1612, 1770
Lsp1109I	GCAGC	9 cut(s)	239, 875, 1089, 1640, 1717, 1751, 1765, 1846, 1919
LweI	GCATC	7 cut(s)	166, 222, 827, 865, 1393, 1921, 2232
MaeI	CTAG	2 cut(s)	1554, 1826
MaeII	ACGT	4 cut(s)	658, 689, 780, 2193
MaeIII	GTNAC	6 cut(s)	200, 659, 1098, 1144, 2092, 2105
MalI	GATC	9 cut(s)	652, 1058, 1578, 1647, 1671, 1914, 1989, 2011, 2190
MboI	GATC	9 cut(s)	650, 1056, 1576, 1645, 1669, 1912, 1987, 2009, 2188
MfeI	CAATTG	2 cut(s)	912, 2325
MflI	RGATCY	1 cut(s)	1912
MhlI	GDGCHC	3 cut(s)	99, 507, 589
MlsI	TGGCCA	3 cut(s)	469, 1887, 2199
MluCI	AATT	7 cut(s)	373, 553, 912, 934, 1981, 1994, 2325
MluNI	TGGCCA	3 cut(s)	469, 1887, 2199
MlyI	GAGTC	5 cut(s)	456, 717, 1706, 1983, 2329
MmeI	TCCRAC	2 cut(s)	424, 1918
Mox20I	TGGCCA	3 cut(s)	469, 1887, 2199
Mph1103I	ATGCAT	1 cut(s)	2278
MroXI	GAANNNNTTC	1 cut(s)	2210
MscI	TGGCCA	3 cut(s)	469, 1887, 2199
MseI	TTAA	8 cut(s)	552, 630, 1206, 1272, 1749, 2082, 2160, 2247
MslI	CAYNNNNRTG	4 cut(s)	992, 1331, 1427, 2281
Msp20I	TGGCCA	3 cut(s)	469, 1887, 2199
MspA1I	CMGCKG	1 cut(s)	1808
MspI	CCGG	3 cut(s)	759, 812, 1971
MspR9I	CCNGG	5 cut(s)	760, 813, 1212, 1611, 1917
MunI	CAATTG	2 cut(s)	912, 2325
Mva1269I	GAATGC	1 cut(s)	724
MvaI	CCWGG	3 cut(s)	1212, 1611, 1917
MvnI	CGCG	1 cut(s)	747
NciI	CCSGG	2 cut(s)	760, 813
NcoI	CCATGG	2 cut(s)	673, 1052
NdeI	CATATG	1 cut(s)	1876
NdeII	GATC	9 cut(s)	650, 1056, 1576, 1645, 1669, 1912, 1987, 2009, 2188
NlaIV	GGNNCC	5 cut(s)	504, 505, 679, 1072, 2071
NmeAIII	GCCGAG	2 cut(s)	318, 731
NmuCI	GTSAC	5 cut(s)	659, 1098, 1144, 2092, 2105
NsiI	ATGCAT	1 cut(s)	2278
NspI	RCATGY	4 cut(s)	843, 859, 974, 1002
PaeI	GCATGC	1 cut(s)	974
PceI	AGGCCT	1 cut(s)	2165
PciI	ACATGT	1 cut(s)	855
PciSI	GCTCTTC	1 cut(s)	2201
PcsI	WCGNNNNNNNCGW	1 cut(s)	655
PctI	GAATGC	1 cut(s)	724
PdmI	GAANNNNTTC	1 cut(s)	2210
PfeI	GAWTC	7 cut(s)	473, 528, 644, 1452, 1847, 2151, 2254
PfoI	TCCNGGA	1 cut(s)	1609
Ple19I	CGATCG	1 cut(s)	653
PleI	GAGTC	5 cut(s)	455, 716, 1705, 1982, 2328
PmaCI	CACGTG	1 cut(s)	659
PmlI	CACGTG	1 cut(s)	659
PpsI	GAGTC	5 cut(s)	455, 716, 1705, 1982, 2328
Ppu21I	YACGTR	1 cut(s)	659
PscI	ACATGT	1 cut(s)	855
Psp124BI	GAGCTC	1 cut(s)	589
Psp6I	CCWGG	3 cut(s)	1210, 1609, 1915
PspCI	CACGTG	1 cut(s)	659
PspEI	GGTNACC	1 cut(s)	2105
PspFI	CCCAGC	3 cut(s)	801, 1045, 1304
PspGI	CCWGG	3 cut(s)	1210, 1609, 1915
PspN4I	GGNNCC	5 cut(s)	504, 505, 679, 1072, 2071
PspOMI	GGGCCC	1 cut(s)	503
PspPI	GGNCC	7 cut(s)	503, 504, 623, 1019, 1084, 1562, 2303
PstI	CTGCAG	1 cut(s)	1967
PstNI	CAGNNNCTG	3 cut(s)	1022, 1610, 1916
PsuI	RGATCY	1 cut(s)	1912
PvuI	CGATCG	1 cut(s)	653
RseI	CAYNNNNRTG	4 cut(s)	992, 1331, 1427, 2281
SacI	GAGCTC	1 cut(s)	589
SapI	GCTCTTC	1 cut(s)	2201
SaqAI	TTAA	8 cut(s)	552, 630, 1206, 1272, 1749, 2082, 2160, 2247
Sau3AI	GATC	9 cut(s)	650, 1056, 1576, 1645, 1669, 1912, 1987, 2009, 2188
Sau96I	GGNCC	7 cut(s)	503, 504, 623, 1019, 1084, 1562, 2303
SchI	GAGTC	5 cut(s)	456, 717, 1706, 1983, 2329
ScrFI	CCNGG	5 cut(s)	760, 813, 1212, 1611, 1917
SduI	GDGCHC	3 cut(s)	99, 507, 589
SfaNI	GCATC	7 cut(s)	166, 222, 827, 865, 1393, 1921, 2232
SfcI	CTRYAG	1 cut(s)	1963
SinI	GGWCC	3 cut(s)	623, 1084, 2303
SmiMI	CAYNNNNRTG	4 cut(s)	992, 1331, 1427, 2281
SmlI	CTYRAG	2 cut(s)	1294, 1589
SmoI	CTYRAG	2 cut(s)	1294, 1589
SpeI	ACTAGT	1 cut(s)	1553
SphI	GCATGC	1 cut(s)	974
Sse9I	AATT	7 cut(s)	373, 553, 912, 934, 1981, 1994, 2325
SseBI	AGGCCT	1 cut(s)	2165
SsiI	CCGC	9 cut(s)	735, 747, 884, 975, 1343, 1733, 1736, 1808, 2141
SspMI	CTAG	2 cut(s)	1554, 1826
SstI	GAGCTC	1 cut(s)	589
StuI	AGGCCT	1 cut(s)	2165
StyD4I	CCNGG	5 cut(s)	758, 811, 1210, 1609, 1915
StyI	CCWWGG	4 cut(s)	673, 822, 1052, 1902
TaiI	ACGT	4 cut(s)	661, 692, 783, 2196
TaqI	TCGA	2 cut(s)	649, 1986
TaqII	GACCGA	1 cut(s)	1101
TasI	AATT	7 cut(s)	373, 553, 912, 934, 1981, 1994, 2325
TatI	WGTACW	3 cut(s)	75, 309, 789
TauI	GCSGC	4 cut(s)	750, 977, 1736, 1739
TfiI	GAWTC	7 cut(s)	473, 528, 644, 1452, 1847, 2151, 2254
Tru1I	TTAA	8 cut(s)	552, 630, 1206, 1272, 1749, 2082, 2160, 2247
Tru9I	TTAA	8 cut(s)	552, 630, 1206, 1272, 1749, 2082, 2160, 2247
TseFI	GTSAC	5 cut(s)	659, 1098, 1144, 2092, 2105
TseI	GCWGC	9 cut(s)	227, 863, 1077, 1628, 1730, 1739, 1778, 1859, 1907
Tsp45I	GTSAC	5 cut(s)	659, 1098, 1144, 2092, 2105
TspDTI	ATGAA	7 cut(s)	7, 17, 135, 261, 947, 1860, 1926
VpaK11BI	GGWCC	3 cut(s)	623, 1084, 2303
XapI	RAATTY	3 cut(s)	934, 1981, 1994
XceI	RCATGY	4 cut(s)	843, 859, 974, 1002
XcmI	CCANNNNNNNNNTGG	3 cut(s)	542, 1453, 1663
XmiI	GTMKAC	1 cut(s)	2121
XmnI	GAANNNNTTC	1 cut(s)	2210
XspI	CTAG	2 cut(s)	1554, 1826
ZraI	GACGTC	1 cut(s)	690
Zsp2I	ATGCAT	1 cut(s)	2278

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.