Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0006347

Physical Location & Seq

Genomic Coordinates Forward (+)

9134593 .. 9141056

Gene Structure 6464 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G008450.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2343 bp

ATGAAGCCAGTTTTCTTCATCATCATATCTCTCCTTCTCCTTCCTCTCTTGGCCTCATGCTCAAAGCAAAAGGTGTACATAGTGTATTTTGGAGAGCACGGTGGAGAGAAAGCATTGCATGAAATAGAGGACAGCCACCACTCCTATTTGCTTTCTGTGAAAGAAACTGAGGAAGATGCCAGAGCTTCACTGCTTTACAGTTACAAGCACAGCATCAATGGCTTTGCAGCAGTTCTCACTGAGAATGAAGCTTCCAAGTTATCTGAACTGGAAGAAGTTGTATCAGTGTGGCCGAGCCACCCAAGAAAGTACACTATGCACACAACAAGGTCATGGGAGTTTGTGGGGATATCGGAAGAAGAGGAAGAGGGGAATTACTGGACGAACAAGATGGCAGGAGACTTCTTATCCAAAGCCAGGTCTGGCAAGAACATCATTGTTGGAGTCCTTGATAGTGGTGTATGGCCAGAATCAAAGAGCTTCAGTGATGTAGGAATGGGGCCCATTCCAAAATCATGGAAGGGAATCTGCCAATCTGGAGTTGGCTTTAATTCGTCCCACTGCAATAGGAAGCTGATAGGAGCTCGGTACTACCTCAAAGGGTTTGAGCAAAACTACGGTCCTCTTAATGCCTCAGAGGATTCTCGATCGCCACGTGACATGGATGGCCATGGAACCCACACGACCTCCACTGTGGCAGGCCGAGTCGTTCCCAATGCCTCAGCCATTGGCGGGTTCGCTCGTGGCTCTGCCTCCGGGGGTGCCCCATTGGCACGCGTTGCTGTGTACAAAGTGTGCTGGGCTATACCGGGCGCATCCAAGGCTGAAGGGAACACATGCTTTGAGGAGGACATGTTTGCAGCCATAGATGATGCCATAGCGGACGGTGTGGATGTGCTGAGCATATCAATTGGCACAACTCGACCTATGAATTTCACCGAGGACGGTATTGCTCTCGGAGCATTGCATGCCGCCAAGAAGAACATTGTGGTGGCATGTAGTGCTGGCAACTCAGGCCCTGCCCCATCTACATTATCAAACCCAGCTCCATGGATCATCACGGTTGGTGCCAGCAGCTTGGACCGAACTTTTGTGTCACCCGTTGTGCTTGGAAATGGCTTGAGCATTGAGGGAGAAACAGTGACCCCAAGCAAGCTGGAAGAGAATAAGATGTACCCTTTAGTATATGCAGGGGATGTTGTTAACCCTGGTGTAGCCCAAAATCTGACAGGACAATGCCTCGCAGGCTCTCTTTCACCTGACAAAGTTAAGGGAAAGATAGTGTTCTGCCGGAGAGGTGCTGGGATGAGAGTTTCAAAAGGCATGGAAGTGAAAAGGGCGGGTGGTGTTGGTTTTATCTTAGGAAACAGCAAAGCAAATGGCGTTGAAATCTCGTGTGACGCTCATGTTCTTCCTGCCACTGCAATGACATACAGTAATGCCAATAGAATCATGGAATACATCAACTCTACCAAAAATCCAAAGGCAACTATTATACCAGCAAGGACAGTGTTGAATACAAAGCCAGCTCCATACATGACTGCTTTCACTAGTAGAGGTCCAAGTACGATTGATCCTAACATTCTCAAGCCTGATATCACAGCTCCTGGACTGAATATATTGGCAGCATGGACTGAGGCTGATCCCCCTACCAAGTTGTCAATGGATCACCGAGTAGCTCAGTATAACATCGAGTCTGGAACTTCAATGTCTTGCCCTCACATTGCTGCAGCGGCAGCACTTCTTAAAGCCATCCACCCTACTTGGAGCAGTGCTGCCATCAAATCGGCTCTAATGACCACAGCGGGGATACGAAACAACCTAGATAAGCTATTGAACGATGAATCGGGCAATGCTGCAACTCCCTTTGCATATGGTGCTGGCCACTTCCGACCCACCAAGGCAGCAGACCCTGGCCTTGTGTACGATGCTTCATACAAAGACTATCTTCTCTACTTCTGCAGTACTGGAGTCAAGAATTTCGATCCAAATTTCAAGTGTCCAAGATCACCACCAACAGCAATCAACCTGAACTATCCTTCCATTGCAATCCCCAAACTGAATGGCACCATCACGATTAAGAGGACAGTCACAAATGTTGGTGACCCCAAAAGCGTTTACTTCTTTACCTCCAAACCGCCTCTTGGAATCTCGGTTAAGGCCTCTCCGAGTATATTGTTCTTTGATCACGTTGGCCAGAGGAAGAGCTTCACCATTACTGTCAAAGCAAGGACGTCAGAGATGCTTAACAAGAATCAGTTGAAAGATGAGTATGCATTTGGGTGGTACACATGGACTGATGGACGTCATATTGTAAGGAGTCCAATTGCAGTCTCTCTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0016787 GO:0017171 GO:0019538 GO:0043170 GO:0044238 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8

Protein Analysis

780

Amino Acids

83.76

Weight (kDa)

8.73

Isoelectric Point (pI)

36.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	25 - 103	7.8e-20	Peptidase inhibitor I9
Peptidase_S8	PF00082	142 - 628	9.4e-41	Subtilase family
PA	PF02225	393 - 484	6.1e-11	PA domain
fn3_6	PF17766	676 - 778	1.2e-29	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006347)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G45650 AT5G45650
fragaria_vesca	FvH4_3g06570 FvH4_3g06570 FvH4_3g06570 FvH4_3g06570 FvH4_3g06570 FvH4_3g06570 FvH4_3g33200
malus_domestica	MD00G1093400.v1.1 MD10G1270100.v1.1
prunus_persica	Prupe.4G071500_v2.0.a1
pyrus_communis	pycom05g27040 pycom10g22620
rosa_chinensis	RchiOBHm_Chr5g0012581
rosa_laevigata	RLG00000031926
rosa_multiflora	Rmu_co8464651.1_g000001 Rmu_sc0002639.1_g000013 Rmu_sc0003577.1_g000010
rosa_roxburghii	Rroxscaffold_1G00064090
rosa_rugosa	Rorug05G0002700 Rorug05G0002700 Rorug05G0002700
rosa_samantha	Rh5AG096500 Rh5CG105600 Rh5DG092200
rosa_wichuraiana	Rw5G008450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	2237, 2308
AccB1I	GGYRCC	3 cut(s)	761, 1067, 2066
AccII	CGCG	1 cut(s)	777
AciI	CCGC	7 cut(s)	732, 881, 972, 1340, 1733, 1805, 2138
AclWI	GGATC	5 cut(s)	1061, 1568, 1637, 1674, 1979
AcoI	YGGCCR	5 cut(s)	290, 464, 667, 1882, 2194
AcsI	RAATTY	3 cut(s)	931, 1978, 1990
AcuI	CTGAAG	2 cut(s)	466, 846
AcvI	CACGTG	1 cut(s)	656
AcyI	GRCGYC	2 cut(s)	2234, 2305
AfaI	GTAC	9 cut(s)	77, 311, 590, 788, 1175, 1567, 1925, 1966, 2288
AfiI	CCNNNNNNNGG	4 cut(s)	417, 732, 1806, 2144
AflIII	ACRYGT	2 cut(s)	775, 852
AgsI	TTSAA	7 cut(s)	1317, 1388, 1516, 1707, 1837, 1996, 2263
AhlI	ACTAGT	1 cut(s)	1550
AjnI	CCWGG	4 cut(s)	416, 1207, 1606, 1912
AloI	GAACNNNNNNTCC	2 cut(s)	2024, 2056
Alw21I	GWGCWC	2 cut(s)	99, 586
Alw26I	GTCTC	2 cut(s)	393, 2338
AlwI	GGATC	5 cut(s)	1061, 1568, 1637, 1674, 1979
AlwNI	CAGNNNCTG	3 cut(s)	1019, 1607, 1913
ApaI	GGGCCC	1 cut(s)	504
ApoI	RAATTY	3 cut(s)	931, 1978, 1990
ArsI	GACNNNNNNTTYG	4 cut(s)	604, 636, 1073, 1105
Asp700I	GAANNNNTTC	1 cut(s)	2207
AspLEI	GCGC	1 cut(s)	815
AspS9I	GGNCC	6 cut(s)	500, 501, 620, 1016, 1081, 1559
AsuC2I	CCSGG	2 cut(s)	757, 810
AsuHPI	GGTGA	7 cut(s)	928, 1089, 1248, 1661, 2001, 2114, 2203
AvaII	GGWCC	3 cut(s)	620, 1081, 1559
BaeGI	GKGCMC	2 cut(s)	504, 766
BalI	TGGCCA	4 cut(s)	466, 669, 1884, 2196
BanI	GGYRCC	3 cut(s)	761, 1067, 2066
BanII	GRGCYC	2 cut(s)	504, 586
BauI	CACGAG	2 cut(s)	741, 1393
BbrPI	CACGTG	1 cut(s)	656
Bbv12I	GWGCWC	2 cut(s)	99, 586
BbvCI	CCTCAGC	1 cut(s)	721
BccI	CCATC	7 cut(s)	385, 659, 1033, 1760, 1787, 2078, 2294
BciT130I	CCWGG	4 cut(s)	418, 1209, 1608, 1914
BciVI	GTATCC	1 cut(s)	1803
BclI	TGATCA	1 cut(s)	2185
BcnI	CCSGG	2 cut(s)	757, 810
BcoDI	GTCTC	2 cut(s)	393, 2338
BcuI	ACTAGT	1 cut(s)	1550
BfaI	CTAG	2 cut(s)	1551, 1823
BfmI	CTRYAG	2 cut(s)	1728, 1960
BfuI	GTATCC	1 cut(s)	1803
BglI	GCCNNNNNGGC	2 cut(s)	770, 1245
BlpI	GCTNAGC	1 cut(s)	899
BmcAI	AGTACT	1 cut(s)	1966
Bme1390I	CCNGG	6 cut(s)	418, 757, 810, 1209, 1608, 1914
Bme18I	GGWCC	3 cut(s)	620, 1081, 1559
BmgT120I	GGNCC	6 cut(s)	500, 501, 620, 1016, 1081, 1559
BmiI	GGNNCC	6 cut(s)	501, 502, 676, 763, 1069, 2068
BmrFI	CCNGG	6 cut(s)	418, 757, 810, 1209, 1608, 1914
BmsI	GCATC	6 cut(s)	166, 222, 824, 862, 1918, 2232
BpmI	CTGGAG	2 cut(s)	558, 1989
Bpu10I	CCTNAGC	1 cut(s)	721
Bpu1102I	GCTNAGC	1 cut(s)	899
BpuEI	CTTGAG	2 cut(s)	1141, 1571
BpuMI	CCSGG	2 cut(s)	757, 810
BsaAI	YACGTR	1 cut(s)	656
BsaHI	GRCGYC	2 cut(s)	2234, 2305
BsaJI	CCNNGG	8 cut(s)	670, 756, 819, 939, 1049, 1207, 1899, 1912
BsaXI	ACNNNNNCTCC	6 cut(s)	671, 701, 839, 869, 1125, 1155
Bsc4I	CCNNNNNNNGG	4 cut(s)	417, 732, 1806, 2144
Bse1I	ACTGG	4 cut(s)	8, 273, 383, 1972
Bse3DI	GCAATG	6 cut(s)	113, 962, 1431, 1722, 1858, 2043
BseBI	CCWGG	4 cut(s)	418, 1209, 1608, 1914
BseDI	CCNNGG	8 cut(s)	670, 756, 819, 939, 1049, 1207, 1899, 1912
BseGI	GGATG	6 cut(s)	670, 815, 898, 1201, 1311, 1752
BseLI	CCNNNNNNNGG	4 cut(s)	417, 732, 1806, 2144
BseMI	GCAATG	6 cut(s)	113, 962, 1431, 1722, 1858, 2043
BseMII	CTCAG	8 cut(s)	159, 231, 648, 735, 890, 1026, 1626, 1694
BseNI	ACTGG	4 cut(s)	8, 273, 383, 1972
BseRI	GAGGAG	1 cut(s)	860
BseSI	GKGCMC	2 cut(s)	504, 766
BseYI	CCCAGC	3 cut(s)	798, 1042, 1301
Bsh1236I	CGCG	1 cut(s)	777
Bsh1285I	CGRYCG	1 cut(s)	650
BshNI	GGYRCC	3 cut(s)	761, 1067, 2066
BsiEI	CGRYCG	1 cut(s)	650
BsiHKAI	GWGCWC	2 cut(s)	99, 586
BsiSI	CCGG	3 cut(s)	756, 809, 1291
BslFI	GGGAC	1 cut(s)	541
BslI	CCNNNNNNNGG	4 cut(s)	417, 732, 1806, 2144
BsmAI	GTCTC	2 cut(s)	393, 2338
BsmFI	GGGAC	1 cut(s)	541
Bsp120I	GGGCCC	1 cut(s)	500
Bsp1286I	GDGCHC	4 cut(s)	99, 504, 586, 766
Bsp1407I	TGTACA	2 cut(s)	75, 786
Bsp143I	GATC	8 cut(s)	647, 1053, 1573, 1642, 1666, 1984, 2006, 2185
Bsp1720I	GCTNAGC	1 cut(s)	899
Bsp19I	CCATGG	2 cut(s)	670, 1049
BspACI	CCGC	7 cut(s)	732, 881, 972, 1340, 1733, 1805, 2138
BspCNI	CTCAG	8 cut(s)	160, 232, 647, 734, 891, 1025, 1627, 1693
BspFNI	CGCG	1 cut(s)	777
BspLI	GGNNCC	6 cut(s)	501, 502, 676, 763, 1069, 2068
BspMAI	CTGCAG	2 cut(s)	1732, 1964
BspPI	GGATC	5 cut(s)	1061, 1568, 1637, 1674, 1979
BspQI	GCTCTTC	1 cut(s)	2198
BspT107I	GGYRCC	3 cut(s)	761, 1067, 2066
BsrDI	GCAATG	6 cut(s)	113, 962, 1431, 1722, 1858, 2043
BsrGI	TGTACA	2 cut(s)	75, 786
BsrI	ACTGG	4 cut(s)	8, 273, 383, 1972
BssECI	CCNNGG	8 cut(s)	670, 756, 819, 939, 1049, 1207, 1899, 1912
BssMI	GATC	8 cut(s)	647, 1053, 1573, 1642, 1666, 1984, 2006, 2185
BssNI	GRCGYC	2 cut(s)	2234, 2305
BssSI	CACGAG	2 cut(s)	741, 1393
BssT1I	CCWWGG	4 cut(s)	670, 819, 1049, 1899
Bst2BI	CACGAG	2 cut(s)	741, 1393
Bst2UI	CCWGG	4 cut(s)	418, 1209, 1608, 1914
Bst6I	CTCTTC	4 cut(s)	354, 360, 1155, 2198
BstACI	GRCGYC	2 cut(s)	2234, 2305
BstAPI	GCANNNNNTGC	4 cut(s)	779, 968, 1001, 1877
BstAUI	TGTACA	2 cut(s)	75, 786
BstBAI	YACGTR	1 cut(s)	656
BstC8I	GCNNGC	9 cut(s)	700, 775, 969, 1006, 1072, 1154, 1246, 1527, 1882
BstDEI	CTNAG	9 cut(s)	168, 240, 634, 721, 899, 1012, 1360, 1635, 1680
BstDSI	CCRYGG	2 cut(s)	670, 1049
BstEII	GGTNACC	1 cut(s)	2102
BstF5I	GGATG	6 cut(s)	670, 815, 898, 1201, 1311, 1752
BstFNI	CGCG	1 cut(s)	777
BstHHI	GCGC	1 cut(s)	815
BstKTI	GATC	8 cut(s)	650, 1056, 1576, 1645, 1669, 1987, 2009, 2188
BstMAI	GTCTC	2 cut(s)	393, 2338
BstMBI	GATC	8 cut(s)	647, 1053, 1573, 1642, 1666, 1984, 2006, 2185
BstMCI	CGRYCG	1 cut(s)	650
BstNI	CCWGG	4 cut(s)	418, 1209, 1608, 1914
BstNSI	RCATGY	4 cut(s)	840, 856, 971, 999
BstPI	GGTNACC	1 cut(s)	2102
BstSCI	CCNGG	6 cut(s)	416, 755, 808, 1207, 1606, 1912
BstSFI	CTRYAG	2 cut(s)	1728, 1960
BstSLI	GKGCMC	2 cut(s)	504, 766
BstUI	CGCG	1 cut(s)	777
BstXI	CCANNNNNNTGG	3 cut(s)	516, 1050, 1078
BsuI	GTATCC	1 cut(s)	1803
BtgI	CCRYGG	2 cut(s)	670, 1049
BtsCI	GGATG	6 cut(s)	670, 815, 898, 1201, 1311, 1752
BtsI	GCAGTG	4 cut(s)	188, 559, 1419, 1777
Cac8I	GCNNGC	9 cut(s)	700, 775, 969, 1006, 1072, 1154, 1246, 1527, 1882
CaiI	CAGNNNCTG	3 cut(s)	1019, 1607, 1913
CfoI	GCGC	1 cut(s)	815
Cfr13I	GGNCC	6 cut(s)	500, 501, 620, 1016, 1081, 1559
CseI	GACGC	1 cut(s)	1409
Csp6I	GTAC	9 cut(s)	76, 310, 589, 787, 1174, 1566, 1924, 1965, 2287
CspCI	CAANNNNNGTGG	2 cut(s)	1745, 1780
CviQI	GTAC	9 cut(s)	76, 310, 589, 787, 1174, 1566, 1924, 1965, 2287
DdeI	CTNAG	9 cut(s)	168, 240, 634, 721, 899, 1012, 1360, 1635, 1680
DpnI	GATC	8 cut(s)	649, 1055, 1575, 1644, 1668, 1986, 2008, 2187
DpnII	GATC	8 cut(s)	647, 1053, 1573, 1642, 1666, 1984, 2006, 2185
EaeI	YGGCCR	5 cut(s)	290, 464, 667, 1882, 2194
Eam1104I	CTCTTC	4 cut(s)	354, 360, 1155, 2198
EarI	CTCTTC	4 cut(s)	354, 360, 1155, 2198
Ecl136II	GAGCTC	1 cut(s)	584
Eco130I	CCWWGG	4 cut(s)	670, 819, 1049, 1899
Eco147I	AGGCCT	1 cut(s)	2162
Eco24I	GRGCYC	2 cut(s)	504, 586
Eco32I	GATATC	2 cut(s)	351, 1597
Eco47I	GGWCC	3 cut(s)	620, 1081, 1559
Eco53kI	GAGCTC	1 cut(s)	584
Eco57I	CTGAAG	2 cut(s)	466, 846
Eco72I	CACGTG	1 cut(s)	656
Eco91I	GGTNACC	1 cut(s)	2102
EcoICRI	GAGCTC	1 cut(s)	584
EcoO109I	RGGNCCY	2 cut(s)	500, 1016
EcoO65I	GGTNACC	1 cut(s)	2102
EcoRII	CCWGG	4 cut(s)	416, 1207, 1606, 1912
EcoRV	GATATC	2 cut(s)	351, 1597
EcoT14I	CCWWGG	4 cut(s)	670, 819, 1049, 1899
EcoT22I	ATGCAT	1 cut(s)	2278
EcoT38I	GRGCYC	2 cut(s)	504, 586
ErhI	CCWWGG	4 cut(s)	670, 819, 1049, 1899
FalI	AAGNNNNNCTT	2 cut(s)	1932, 1964
FaqI	GGGAC	1 cut(s)	541
FauI	CCCGC	3 cut(s)	725, 1333, 1798
FauNDI	CATATG	1 cut(s)	1873
FbaI	TGATCA	1 cut(s)	2185
FokI	GGATG	6 cut(s)	677, 802, 905, 1208, 1318, 1739
FriOI	GRGCYC	2 cut(s)	504, 586
FspBI	CTAG	2 cut(s)	1551, 1823
GlaI	GCGC	1 cut(s)	814
GsaI	CCCAGC	3 cut(s)	802, 1046, 1305
GsuI	CTGGAG	2 cut(s)	558, 1989
HapII	CCGG	3 cut(s)	756, 809, 1291
HgaI	GACGC	1 cut(s)	1409
HhaI	GCGC	1 cut(s)	815
Hin1I	GRCGYC	2 cut(s)	2234, 2305
Hin6I	GCGC	1 cut(s)	813
HinP1I	GCGC	1 cut(s)	813
HincII	GTYRAC	1 cut(s)	1204
HindII	GTYRAC	1 cut(s)	1204
HindIII	AAGCTT	1 cut(s)	249
HpaI	GTTAAC	1 cut(s)	1204
HpaII	CCGG	3 cut(s)	756, 809, 1291
HphI	GGTGA	7 cut(s)	928, 1089, 1248, 1661, 2001, 2114, 2203
Hpy166II	GTNNAC	7 cut(s)	76, 312, 787, 1204, 1924, 2119, 2289
Hpy188I	TCNGA	8 cut(s)	265, 355, 637, 959, 1227, 1892, 2169, 2239
Hpy188III	TCNNGA	5 cut(s)	537, 645, 1698, 1975, 2074
Hpy8I	GTNNAC	7 cut(s)	76, 312, 787, 1204, 1924, 2119, 2289
HpyAV	CCTTC	5 cut(s)	44, 50, 514, 821, 2049
HpyCH4IV	ACGT	4 cut(s)	655, 2190, 2234, 2305
HpyF3I	CTNAG	9 cut(s)	168, 240, 634, 721, 899, 1012, 1360, 1635, 1680
HpySE526I	ACGT	4 cut(s)	655, 2190, 2234, 2305
Hsp92I	GRCGYC	2 cut(s)	2234, 2305
HspAI	GCGC	1 cut(s)	813
Ksp22I	TGATCA	1 cut(s)	2185
KspAI	GTTAAC	1 cut(s)	1204
Kzo9I	GATC	8 cut(s)	647, 1053, 1573, 1642, 1666, 1984, 2006, 2185
LguI	GCTCTTC	1 cut(s)	2198
LmnI	GCTCC	6 cut(s)	581, 959, 1051, 1534, 1609, 1767
LweI	GCATC	6 cut(s)	166, 222, 824, 862, 1918, 2232
MaeI	CTAG	2 cut(s)	1551, 1823
MaeII	ACGT	4 cut(s)	655, 2190, 2234, 2305
MaeIII	GTNAC	7 cut(s)	200, 656, 1095, 1141, 1397, 2089, 2102
MalI	GATC	8 cut(s)	649, 1055, 1575, 1644, 1668, 1986, 2008, 2187
MboI	GATC	8 cut(s)	647, 1053, 1573, 1642, 1666, 1984, 2006, 2185
MfeI	CAATTG	2 cut(s)	909, 2325
MhlI	GDGCHC	4 cut(s)	99, 504, 586, 766
MlsI	TGGCCA	4 cut(s)	466, 669, 1884, 2196
MluCI	AATT	7 cut(s)	373, 550, 909, 931, 1978, 1990, 2325
MluI	ACGCGT	1 cut(s)	775
MluNI	TGGCCA	4 cut(s)	466, 669, 1884, 2196
MlyI	GAGTC	5 cut(s)	453, 714, 1703, 1980, 2329
MmeI	TCCRAC	2 cut(s)	421, 1915
Mox20I	TGGCCA	4 cut(s)	466, 669, 1884, 2196
Mph1103I	ATGCAT	1 cut(s)	2278
MroXI	GAANNNNTTC	1 cut(s)	2207
MscI	TGGCCA	4 cut(s)	466, 669, 1884, 2196
MseI	TTAA	8 cut(s)	549, 627, 1203, 1269, 1746, 2079, 2157, 2247
MslI	CAYNNNNRTG	4 cut(s)	989, 1328, 1424, 2281
Msp20I	TGGCCA	4 cut(s)	466, 669, 1884, 2196
MspA1I	CMGCKG	2 cut(s)	1733, 1805
MspI	CCGG	3 cut(s)	756, 809, 1291
MspR9I	CCNGG	6 cut(s)	418, 757, 810, 1209, 1608, 1914
MunI	CAATTG	2 cut(s)	909, 2325
MvaI	CCWGG	4 cut(s)	418, 1209, 1608, 1914
MvnI	CGCG	1 cut(s)	777
NciI	CCSGG	2 cut(s)	757, 810
NcoI	CCATGG	2 cut(s)	670, 1049
NdeI	CATATG	1 cut(s)	1873
NdeII	GATC	8 cut(s)	647, 1053, 1573, 1642, 1666, 1984, 2006, 2185
NlaIV	GGNNCC	6 cut(s)	501, 502, 676, 763, 1069, 2068
NmeAIII	GCCGAG	2 cut(s)	318, 728
NmuCI	GTSAC	6 cut(s)	656, 1095, 1141, 1397, 2089, 2102
NsiI	ATGCAT	1 cut(s)	2278
NspI	RCATGY	4 cut(s)	840, 856, 971, 999
PaeI	GCATGC	1 cut(s)	971
PceI	AGGCCT	1 cut(s)	2162
PciI	ACATGT	1 cut(s)	852
PciSI	GCTCTTC	1 cut(s)	2198
PcsI	WCGNNNNNNNCGW	1 cut(s)	652
PdmI	GAANNNNTTC	1 cut(s)	2207
PfeI	GAWTC	7 cut(s)	470, 525, 641, 1449, 1844, 2148, 2254
PfoI	TCCNGGA	1 cut(s)	1606
Ple19I	CGATCG	1 cut(s)	650
PleI	GAGTC	5 cut(s)	452, 713, 1702, 1979, 2328
PmaCI	CACGTG	1 cut(s)	656
PmlI	CACGTG	1 cut(s)	656
PpsI	GAGTC	5 cut(s)	452, 713, 1702, 1979, 2328
Ppu21I	YACGTR	1 cut(s)	656
PscI	ACATGT	1 cut(s)	852
Psp124BI	GAGCTC	1 cut(s)	586
Psp6I	CCWGG	4 cut(s)	416, 1207, 1606, 1912
PspCI	CACGTG	1 cut(s)	656
PspEI	GGTNACC	1 cut(s)	2102
PspFI	CCCAGC	3 cut(s)	798, 1042, 1301
PspGI	CCWGG	4 cut(s)	416, 1207, 1606, 1912
PspN4I	GGNNCC	6 cut(s)	501, 502, 676, 763, 1069, 2068
PspOMI	GGGCCC	1 cut(s)	500
PspPI	GGNCC	6 cut(s)	500, 501, 620, 1016, 1081, 1559
PstI	CTGCAG	2 cut(s)	1732, 1964
PstNI	CAGNNNCTG	3 cut(s)	1019, 1607, 1913
PvuI	CGATCG	1 cut(s)	650
RsaI	GTAC	9 cut(s)	77, 311, 590, 788, 1175, 1567, 1925, 1966, 2288
RsaNI	GTAC	9 cut(s)	76, 310, 589, 787, 1174, 1566, 1924, 1965, 2287
RseI	CAYNNNNRTG	4 cut(s)	989, 1328, 1424, 2281
SacI	GAGCTC	1 cut(s)	586
SapI	GCTCTTC	1 cut(s)	2198
SaqAI	TTAA	8 cut(s)	549, 627, 1203, 1269, 1746, 2079, 2157, 2247
Sau3AI	GATC	8 cut(s)	647, 1053, 1573, 1642, 1666, 1984, 2006, 2185
Sau96I	GGNCC	6 cut(s)	500, 501, 620, 1016, 1081, 1559
ScaI	AGTACT	1 cut(s)	1966
SchI	GAGTC	5 cut(s)	453, 714, 1703, 1980, 2329
ScrFI	CCNGG	6 cut(s)	418, 757, 810, 1209, 1608, 1914
SduI	GDGCHC	4 cut(s)	99, 504, 586, 766
SfaNI	GCATC	6 cut(s)	166, 222, 824, 862, 1918, 2232
SfcI	CTRYAG	2 cut(s)	1728, 1960
SinI	GGWCC	3 cut(s)	620, 1081, 1559
SmiMI	CAYNNNNRTG	4 cut(s)	989, 1328, 1424, 2281
SmlI	CTYRAG	2 cut(s)	1120, 1586
SmoI	CTYRAG	2 cut(s)	1120, 1586
SpeI	ACTAGT	1 cut(s)	1550
SphI	GCATGC	1 cut(s)	971
Sse9I	AATT	7 cut(s)	373, 550, 909, 931, 1978, 1990, 2325
SseBI	AGGCCT	1 cut(s)	2162
SsiI	CCGC	7 cut(s)	732, 881, 972, 1340, 1733, 1805, 2138
SspMI	CTAG	2 cut(s)	1551, 1823
SstI	GAGCTC	1 cut(s)	586
StuI	AGGCCT	1 cut(s)	2162
StyD4I	CCNGG	6 cut(s)	416, 755, 808, 1207, 1606, 1912
StyI	CCWWGG	4 cut(s)	670, 819, 1049, 1899
TaiI	ACGT	4 cut(s)	658, 2193, 2237, 2308
TaqI	TCGA	4 cut(s)	646, 922, 1692, 1983
TaqII	GACCGA	1 cut(s)	1098
TasI	AATT	7 cut(s)	373, 550, 909, 931, 1978, 1990, 2325
TatI	WGTACW	4 cut(s)	75, 309, 786, 1964
TauI	GCSGC	2 cut(s)	974, 1736
TfiI	GAWTC	7 cut(s)	470, 525, 641, 1449, 1844, 2148, 2254
Tru1I	TTAA	8 cut(s)	549, 627, 1203, 1269, 1746, 2079, 2157, 2247
Tru9I	TTAA	8 cut(s)	549, 627, 1203, 1269, 1746, 2079, 2157, 2247
TseFI	GTSAC	6 cut(s)	656, 1095, 1141, 1397, 2089, 2102
Tsp45I	GTSAC	6 cut(s)	656, 1095, 1141, 1397, 2089, 2102
TspDTI	ATGAA	7 cut(s)	7, 17, 135, 261, 944, 1857, 1923
VpaK11BI	GGWCC	3 cut(s)	620, 1081, 1559
XapI	RAATTY	3 cut(s)	931, 1978, 1990
XceI	RCATGY	4 cut(s)	840, 856, 971, 999
XcmI	CCANNNNNNNNNTGG	3 cut(s)	539, 1450, 1660
XmnI	GAANNNNTTC	1 cut(s)	2207
XspI	CTAG	2 cut(s)	1551, 1823
ZraI	GACGTC	2 cut(s)	2235, 2306
ZrmI	AGTACT	1 cut(s)	1966
Zsp2I	ATGCAT	1 cut(s)	2278

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.