Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0006347

Physical Location & Seq

Genomic Coordinates Reverse (-)

36232214 .. 36237045

Gene Structure 4832 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1270100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1779 bp

ATGTGGAAGCTGATAGGAGCTCGCTACTACCTCAAAGGTTTTGAGCAATTCTACGGTCATTTAAATGCCTCGGAGGATTACCGATCACCTCGTGATATGGATGGCCACGGAACACACACAAGCTCAACCGTTGCAGGCCGAGTTGTTCCCAACACCTCAGCCCTGGGTGGTTTTGCCCTAGGCACGGCCTCTGGCGGTGCCCCATTGGCACGCCTTGCCATATACAAAGTGTGTTGGGCAATACCAGGCCAGTCCAAGGCAGATGGCAACACATGCTTTGAGGAAGACATGTTGGCAGCCATTGATGATGCAATAGGTGATGGTGTTGATGTACTGAGCATATCAATTGGGACCAAAAAACCTGTAAATTACACCAATGATGGCATTGCTATTGGAGCATTGCATGCCACCAAGAAGAACATTGTGGTGGCATGTAGTGCTGGGAACAATGGCCCTGCTCCATCTACTCTTTCAAATCCGGCTCCATGGATAATCACAGTTGGTGCTAGCAGCCTCGACCGGGCATTCGTCGCTCCGGTTGTGCTTGGAAATGGGATCACAATTGAGGGAGAAACAGTGACACCAAGCAAGCTGGAAGAGAATAAGAAGTACCCTTTAGTATATGCTGCAGATGTTATTAACTCTGGTGTACCAAAAGATATGGCAGGACAATGCCTACCAGGCTCTCTTTCACCTGAGAAGGTGAAGGGAAATATAGTTTTGTGCATGAGAGGAAGTGGGATGAGAGTTGGAAAAGGCATGGAAGTGAAAAGGGCAGGAGGAGTTGGATTTATATTAGGAAACAGTGCAGCAAATGGGGTTGAAATTTCCTGTGATGCTCATGTTATTCCTGCCACTGCAGTGCTTTATGAAGATGCCGATAGAATTCTTCAATACATCAACTCTACCAAAATTCCAACGGCTACTATTATACCAGCAAGGACTGTTTTCAATAGAAAGCCAGCTCCCTTCATGACTGCATTCACTAGTAGAGGCCCCAATCTCATTGACCCTAACTTTCTCAAGCCAGATATCACAGCTCCAGGGCTGAATATATTGGCGGCGTGGACTGAGGCTGATCCGCCAACCAAGTTGTCCGGTATAGATCATCGAGTTGTTCGGTATAATATATACTCTGGAACTTCAATGTCTTGCCCTCATGTAGCAGCTGCAGCAGTACTTGTCAAAGCAATCCACCCTACTTGGAGCAGTGCTGCCATAAGATCTGCTATCATGACCACAGCTGAAATAAGAAACAAATTGGGTTTACCATTGAAAGATGAATCAGGAAGTGTTGCTACTCCCTTTGCATATGGGGCCGGACACTTTCGTCCCACGAAGGCAGCAGACCCCGGCCTTGTATACGATGCTTCGTACAAGGACTATCTTCAATACATTTGCAGTATAGGAGTAGCCAAGAACGTCGATCCAACATTCAATTGTCCAAAATCACCACCAACAGCAATCAACCTGAACTACCCTTCCATTGCAATCCCCAAACTAAAGGACACCATGACCATCAAGAGGACAGTCACAAATGTTGGCGACAGCAAAAGCATTTACTTCTTTACCGCCAAGCCGCCTTTGGGAATCTCCATTAAGGCATCCCCGAGCATGTTGTTCTTCGATCATTTCGGCCAGAAGAAGAGCTTCACCGTAACAGTTAAAGCAAGAAGAGAGATGCTTAGCAAGCAAGGCAATGAGTATGTCTTTGGGTGGTACACTTGGACCGATGGACTCCACACTGTGAGGAGTCCCATTGCAGTGTCTTTAGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0016787 GO:0017171 GO:0019538 GO:0043170 GO:0044238 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8

Protein Analysis

593

Amino Acids

62.97

Weight (kDa)

9.13

Isoelectric Point (pI)

32.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_S8	PF00082	21 - 441	1.5e-37	Subtilase family
PA	PF02225	203 - 296	1.8e-11	PA domain
fn3_6	PF17766	490 - 589	2.5e-30	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0006347)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G45650 AT5G45650
fragaria_vesca	FvH4_3g06570 FvH4_3g06570 FvH4_3g06570 FvH4_3g06570 FvH4_3g06570 FvH4_3g06570 FvH4_3g33200
malus_domestica	MD00G1093400.v1.1 MD10G1270100.v1.1
prunus_persica	Prupe.4G071500_v2.0.a1
pyrus_communis	pycom05g27040 pycom10g22620
rosa_chinensis	RchiOBHm_Chr5g0012581
rosa_laevigata	RLG00000031926
rosa_multiflora	Rmu_co8464651.1_g000001 Rmu_sc0002639.1_g000013 Rmu_sc0003577.1_g000010
rosa_roxburghii	Rroxscaffold_1G00064090
rosa_rugosa	Rorug05G0002700 Rorug05G0002700 Rorug05G0002700
rosa_samantha	Rh5AG096500 Rh5CG105600 Rh5DG092200
rosa_wichuraiana	Rw5G008450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1329
AccB1I	GGYRCC	1 cut(s)	197
AccI	GTMKAC	1 cut(s)	1362
AciI	CCGC	5 cut(s)	195, 1061, 1082, 1572, 1580
AclWI	GGATC	3 cut(s)	563, 1073, 1421
AcoI	YGGCCR	2 cut(s)	103, 1636
AcsI	RAATTY	3 cut(s)	825, 885, 912
AdeI	CACNNNGTG	2 cut(s)	92, 1747
AfaI	GTAC	6 cut(s)	333, 611, 651, 1179, 1376, 1721
AfiI	CCNNNNNNNGG	5 cut(s)	184, 256, 520, 1524, 1586
AflIII	ACRYGT	1 cut(s)	288
AgsI	TTSAA	8 cut(s)	474, 824, 893, 952, 1146, 1276, 1391, 1438
AhlI	ACTAGT	1 cut(s)	986
AjnI	CCWGG	4 cut(s)	162, 244, 679, 1042
AleI	CACNNNNGTG	1 cut(s)	860
AloI	GAACNNNNNNTCC	2 cut(s)	1466, 1498
AluBI	AGCT	9 cut(s)	10, 20, 123, 592, 965, 1040, 1169, 1244, 1650
AluI	AGCT	9 cut(s)	10, 20, 123, 592, 965, 1040, 1169, 1244, 1650
Alw21I	GWGCWC	1 cut(s)	22
AlwI	GGATC	3 cut(s)	563, 1073, 1421
Ama87I	CYCGRG	1 cut(s)	1609
AoxI	GGCC	9 cut(s)	103, 136, 186, 247, 451, 994, 1317, 1354, 1636
ApeKI	GCWGC	9 cut(s)	296, 510, 626, 809, 1166, 1169, 1172, 1214, 1343
ApoI	RAATTY	3 cut(s)	825, 885, 912
ArsI	GACNNNNNNTTYG	2 cut(s)	509, 541
Asp700I	GAANNNNTTC	1 cut(s)	1649
AspA2I	CCTAGG	1 cut(s)	178
AspS9I	GGNCC	5 cut(s)	351, 452, 995, 1317, 1728
AsuC2I	CCSGG	2 cut(s)	521, 1353
AsuHPI	GGTGA	6 cut(s)	78, 329, 684, 715, 1443, 1645
AsuNHI	GCTAGC	1 cut(s)	506
AvaI	CYCGRG	1 cut(s)	1609
AvaII	GGWCC	2 cut(s)	351, 1728
AvrII	CCTAGG	1 cut(s)	178
BaeGI	GKGCMC	1 cut(s)	202
BalI	TGGCCA	1 cut(s)	105
BanI	GGYRCC	1 cut(s)	197
BanII	GRGCYC	1 cut(s)	22
BarI	GAAGNNNNNNTAC	2 cut(s)	1282, 1314
BauI	CACGAG	1 cut(s)	90
BbsI	GAAGAC	1 cut(s)	291
Bbv12I	GWGCWC	1 cut(s)	22
BbvCI	CCTCAGC	1 cut(s)	157
BbvI	GCAGC	9 cut(s)	308, 522, 613, 821, 1156, 1178, 1184, 1201, 1355
BccI	CCATC	7 cut(s)	95, 257, 314, 374, 469, 1526, 1727
BceAI	ACGGC	2 cut(s)	201, 936
BciT130I	CCWGG	4 cut(s)	164, 246, 681, 1044
BcnI	CCSGG	2 cut(s)	521, 1353
BcuI	ACTAGT	1 cut(s)	986
BfaI	CTAG	3 cut(s)	179, 507, 987
BfmI	CTRYAG	3 cut(s)	627, 858, 1170
BglI	GCCNNNNNGGC	2 cut(s)	206, 681
BglII	AGATCT	1 cut(s)	1223
BlnI	CCTAGG	1 cut(s)	178
BlpI	GCTNAGC	1 cut(s)	1685
BmcAI	AGTACT	1 cut(s)	1179
Bme1390I	CCNGG	6 cut(s)	164, 246, 521, 681, 1044, 1353
Bme18I	GGWCC	2 cut(s)	351, 1728
BmeT110I	CYCGRG	1 cut(s)	1609
BmgT120I	GGNCC	5 cut(s)	351, 452, 995, 1317, 1728
BmiI	GGNNCC	5 cut(s)	199, 352, 483, 997, 1318
BmrFI	CCNGG	6 cut(s)	164, 246, 521, 681, 1044, 1353
BmsI	GCATC	6 cut(s)	298, 826, 865, 1357, 1613, 1671
BmtI	GCTAGC	1 cut(s)	510
BpiI	GAAGAC	1 cut(s)	291
BpmI	CTGGAG	1 cut(s)	1026
Bpu10I	CCTNAGC	1 cut(s)	157
Bpu1102I	GCTNAGC	1 cut(s)	1685
BpuEI	CTTGAG	1 cut(s)	1007
BpuMI	CCSGG	2 cut(s)	521, 1353
BsaJI	CCNNGG	9 cut(s)	69, 106, 162, 163, 178, 255, 485, 1043, 1351
BsaWI	WCCGGW	2 cut(s)	535, 1097
BsaXI	ACNNNNNCTCC	2 cut(s)	561, 591
Bsc4I	CCNNNNNNNGG	5 cut(s)	184, 256, 520, 1524, 1586
Bse1I	ACTGG	1 cut(s)	250
Bse3DI	GCAATG	5 cut(s)	384, 398, 1485, 1705, 1758
BseBI	CCWGG	4 cut(s)	164, 246, 681, 1044
BseDI	CCNNGG	9 cut(s)	69, 106, 162, 163, 178, 255, 485, 1043, 1351
BseGI	GGATG	3 cut(s)	106, 747, 1604
BseLI	CCNNNNNNNGG	5 cut(s)	184, 256, 520, 1524, 1586
BseMI	GCAATG	5 cut(s)	384, 398, 1485, 1705, 1758
BseMII	CTCAG	4 cut(s)	171, 326, 687, 1062
BseNI	ACTGG	1 cut(s)	250
BseRI	GAGGAG	2 cut(s)	795, 1765
BseSI	GKGCMC	1 cut(s)	202
BseXI	GCAGC	9 cut(s)	308, 522, 613, 821, 1156, 1178, 1184, 1201, 1355
BseYI	CCCAGC	1 cut(s)	440
BsgI	GTGCAG	1 cut(s)	828
Bsh1285I	CGRYCG	1 cut(s)	520
BshFI	GGCC	9 cut(s)	105, 138, 188, 249, 453, 996, 1319, 1356, 1638
BshNI	GGYRCC	1 cut(s)	197
BsiEI	CGRYCG	1 cut(s)	520
BsiHKAI	GWGCWC	1 cut(s)	22
BsiHKCI	CYCGRG	1 cut(s)	1609
BsiSI	CCGG	6 cut(s)	479, 520, 536, 1098, 1320, 1353
BslFI	GGGAC	3 cut(s)	364, 1317, 1740
BslI	CCNNNNNNNGG	5 cut(s)	184, 256, 520, 1524, 1586
BsmFI	GGGAC	3 cut(s)	364, 1317, 1740
BsmI	GAATGC	2 cut(s)	524, 980
BsnI	GGCC	9 cut(s)	105, 138, 188, 249, 453, 996, 1319, 1356, 1638
BsoBI	CYCGRG	1 cut(s)	1609
Bsp1286I	GDGCHC	2 cut(s)	22, 202
Bsp143I	GATC	7 cut(s)	83, 555, 1078, 1105, 1223, 1426, 1627
Bsp1720I	GCTNAGC	1 cut(s)	1685
Bsp19I	CCATGG	1 cut(s)	485
BspACI	CCGC	5 cut(s)	195, 1061, 1082, 1572, 1580
BspANI	GGCC	9 cut(s)	105, 138, 188, 249, 453, 996, 1319, 1356, 1638
BspCNI	CTCAG	4 cut(s)	170, 327, 688, 1063
BspHI	TCATGA	2 cut(s)	972, 1233
BspLI	GGNNCC	5 cut(s)	199, 352, 483, 997, 1318
BspMAI	CTGCAG	3 cut(s)	631, 862, 1174
BspOI	GCTAGC	1 cut(s)	510
BspPI	GGATC	3 cut(s)	563, 1073, 1421
BspQI	GCTCTTC	1 cut(s)	1640
BspT107I	GGYRCC	1 cut(s)	197
BsrDI	GCAATG	5 cut(s)	384, 398, 1485, 1705, 1758
BsrI	ACTGG	1 cut(s)	250
BssECI	CCNNGG	9 cut(s)	69, 106, 162, 163, 178, 255, 485, 1043, 1351
BssMI	GATC	7 cut(s)	83, 555, 1078, 1105, 1223, 1426, 1627
BssNAI	GTATAC	1 cut(s)	1363
BssSI	CACGAG	1 cut(s)	90
BssT1I	CCWWGG	3 cut(s)	178, 255, 485
Bst1107I	GTATAC	1 cut(s)	1363
Bst2BI	CACGAG	1 cut(s)	90
Bst2UI	CCWGG	4 cut(s)	164, 246, 681, 1044
Bst6I	CTCTTC	3 cut(s)	591, 1640, 1669
BstAPI	GCANNNNNTGC	4 cut(s)	215, 273, 404, 437
BstC8I	GCNNGC	8 cut(s)	22, 136, 211, 405, 508, 590, 963, 1691
BstDEI	CTNAG	5 cut(s)	157, 335, 696, 1071, 1685
BstDSI	CCRYGG	2 cut(s)	106, 485
BstF5I	GGATG	3 cut(s)	106, 747, 1604
BstKTI	GATC	7 cut(s)	86, 558, 1081, 1108, 1226, 1429, 1630
BstMBI	GATC	7 cut(s)	83, 555, 1078, 1105, 1223, 1426, 1627
BstMCI	CGRYCG	1 cut(s)	520
BstNI	CCWGG	4 cut(s)	164, 246, 681, 1044
BstNSI	RCATGY	5 cut(s)	276, 292, 407, 435, 1618
BstSCI	CCNGG	6 cut(s)	162, 244, 519, 679, 1042, 1351
BstSFI	CTRYAG	3 cut(s)	627, 858, 1170
BstSLI	GKGCMC	1 cut(s)	202
BstV1I	GCAGC	9 cut(s)	308, 522, 613, 821, 1156, 1178, 1184, 1201, 1355
BstV2I	GAAGAC	1 cut(s)	291
BstX2I	RGATCY	1 cut(s)	1223
BstYI	RGATCY	1 cut(s)	1223
BstZ17I	GTATAC	1 cut(s)	1363
BsuRI	GGCC	9 cut(s)	105, 138, 188, 249, 453, 996, 1319, 1356, 1638
BtgI	CCRYGG	2 cut(s)	106, 485
BtsCI	GGATG	3 cut(s)	106, 747, 1604
BtsI	GCAGTG	4 cut(s)	855, 867, 1216, 1770
BtsIMutI	CAGTG	7 cut(s)	582, 811, 855, 867, 1216, 1743, 1770
Cac8I	GCNNGC	8 cut(s)	22, 136, 211, 405, 508, 590, 963, 1691
CciI	TCATGA	2 cut(s)	972, 1233
Cfr13I	GGNCC	5 cut(s)	351, 452, 995, 1317, 1728
Csp6I	GTAC	6 cut(s)	332, 610, 650, 1178, 1375, 1720
CspCI	CAANNNNNGTGG	2 cut(s)	1184, 1219
CviQI	GTAC	6 cut(s)	332, 610, 650, 1178, 1375, 1720
DdeI	CTNAG	5 cut(s)	157, 335, 696, 1071, 1685
DpnI	GATC	7 cut(s)	85, 557, 1080, 1107, 1225, 1428, 1629
DpnII	GATC	7 cut(s)	83, 555, 1078, 1105, 1223, 1426, 1627
DraI	TTTAAA	1 cut(s)	63
DraIII	CACNNNGTG	2 cut(s)	92, 1747
DrdI	GACNNNNNNGTC	1 cut(s)	1329
DseDI	GACNNNNNNGTC	1 cut(s)	1329
EaeI	YGGCCR	2 cut(s)	103, 1636
Eam1104I	CTCTTC	3 cut(s)	591, 1640, 1669
EarI	CTCTTC	3 cut(s)	591, 1640, 1669
EciI	GGCGGA	1 cut(s)	1071
Ecl136II	GAGCTC	1 cut(s)	20
Eco130I	CCWWGG	3 cut(s)	178, 255, 485
Eco24I	GRGCYC	1 cut(s)	22
Eco32I	GATATC	1 cut(s)	1033
Eco47I	GGWCC	2 cut(s)	351, 1728
Eco53kI	GAGCTC	1 cut(s)	20
Eco88I	CYCGRG	1 cut(s)	1609
EcoICRI	GAGCTC	1 cut(s)	20
EcoO109I	RGGNCCY	1 cut(s)	995
EcoRI	GAATTC	1 cut(s)	885
EcoRII	CCWGG	4 cut(s)	162, 244, 679, 1042
EcoRV	GATATC	1 cut(s)	1033
EcoT14I	CCWWGG	3 cut(s)	178, 255, 485
EcoT38I	GRGCYC	1 cut(s)	22
ErhI	CCWWGG	3 cut(s)	178, 255, 485
FalI	AAGNNNNNCTT	2 cut(s)	1634, 1666
FaqI	GGGAC	3 cut(s)	364, 1317, 1740
FauNDI	CATATG	1 cut(s)	1312
FblI	GTMKAC	1 cut(s)	1362
FokI	GGATG	3 cut(s)	113, 754, 1591
FriOI	GRGCYC	1 cut(s)	22
FspBI	CTAG	3 cut(s)	179, 507, 987
GsaI	CCCAGC	1 cut(s)	444
GsuI	CTGGAG	1 cut(s)	1026
HaeIII	GGCC	9 cut(s)	105, 138, 188, 249, 453, 996, 1319, 1356, 1638
HapII	CCGG	6 cut(s)	479, 520, 536, 1098, 1320, 1353
HinfI	GANTC	4 cut(s)	1283, 1590, 1737, 1753
HpaII	CCGG	6 cut(s)	479, 520, 536, 1098, 1320, 1353
HphI	GGTGA	6 cut(s)	78, 329, 684, 715, 1443, 1645
Hpy166II	GTNNAC	5 cut(s)	650, 1068, 1268, 1363, 1722
Hpy188I	TCNGA	1 cut(s)	73
Hpy188III	TCNNGA	6 cut(s)	92, 973, 1137, 1234, 1287, 1522
Hpy8I	GTNNAC	5 cut(s)	650, 1068, 1268, 1363, 1722
Hpy99I	CGWCG	2 cut(s)	533, 1427
HpyAV	CCTTC	5 cut(s)	694, 700, 979, 1333, 1491
HpyCH4IV	ACGT	1 cut(s)	1422
HpyF3I	CTNAG	5 cut(s)	157, 335, 696, 1071, 1685
HpySE526I	ACGT	1 cut(s)	1422
Kzo9I	GATC	7 cut(s)	83, 555, 1078, 1105, 1223, 1426, 1627
LguI	GCTCTTC	1 cut(s)	1640
LmnI	GCTCC	8 cut(s)	17, 395, 463, 487, 538, 970, 1045, 1206
Lsp1109I	GCAGC	9 cut(s)	308, 522, 613, 821, 1156, 1178, 1184, 1201, 1355
LweI	GCATC	6 cut(s)	298, 826, 865, 1357, 1613, 1671
MaeI	CTAG	3 cut(s)	179, 507, 987
MaeII	ACGT	1 cut(s)	1422
MaeIII	GTNAC	3 cut(s)	577, 1531, 1657
MalI	GATC	7 cut(s)	85, 557, 1080, 1107, 1225, 1428, 1629
MboI	GATC	7 cut(s)	83, 555, 1078, 1105, 1223, 1426, 1627
MfeI	CAATTG	3 cut(s)	345, 561, 1438
MflI	RGATCY	1 cut(s)	1223
MhlI	GDGCHC	2 cut(s)	22, 202
MlsI	TGGCCA	1 cut(s)	105
MluCI	AATT	9 cut(s)	47, 345, 367, 561, 825, 885, 912, 1259, 1438
MluNI	TGGCCA	1 cut(s)	105
MlyI	GAGTC	2 cut(s)	1731, 1762
MmeI	TCCRAC	4 cut(s)	730, 766, 941, 1454
Mox20I	TGGCCA	1 cut(s)	105
MroXI	GAANNNNTTC	1 cut(s)	1649
MscI	TGGCCA	1 cut(s)	105
MseI	TTAA	4 cut(s)	62, 639, 1599, 1665
MslI	CAYNNNNRTG	5 cut(s)	63, 425, 764, 860, 1763
Msp20I	TGGCCA	1 cut(s)	105
MspA1I	CMGCKG	2 cut(s)	1169, 1244
MspI	CCGG	6 cut(s)	479, 520, 536, 1098, 1320, 1353
MspR9I	CCNGG	6 cut(s)	164, 246, 521, 681, 1044, 1353
MunI	CAATTG	3 cut(s)	345, 561, 1438
Mva1269I	GAATGC	2 cut(s)	524, 980
MvaI	CCWGG	4 cut(s)	164, 246, 681, 1044
NciI	CCSGG	2 cut(s)	521, 1353
NcoI	CCATGG	1 cut(s)	485
NdeI	CATATG	1 cut(s)	1312
NdeII	GATC	7 cut(s)	83, 555, 1078, 1105, 1223, 1426, 1627
NheI	GCTAGC	1 cut(s)	506
NlaIV	GGNNCC	5 cut(s)	199, 352, 483, 997, 1318
NmeAIII	GCCGAG	1 cut(s)	164
NmuCI	GTSAC	2 cut(s)	577, 1531
NspI	RCATGY	5 cut(s)	276, 292, 407, 435, 1618
OliI	CACNNNNGTG	1 cut(s)	860
PaeI	GCATGC	1 cut(s)	407
PagI	TCATGA	2 cut(s)	972, 1233
PasI	CCCWGGG	1 cut(s)	163
PciI	ACATGT	1 cut(s)	288
PciSI	GCTCTTC	1 cut(s)	1640
PctI	GAATGC	2 cut(s)	524, 980
PdmI	GAANNNNTTC	1 cut(s)	1649
PfeI	GAWTC	2 cut(s)	1283, 1590
PleI	GAGTC	2 cut(s)	1731, 1761
PpsI	GAGTC	2 cut(s)	1731, 1761
PscI	ACATGT	1 cut(s)	288
Psp124BI	GAGCTC	1 cut(s)	22
Psp6I	CCWGG	4 cut(s)	162, 244, 679, 1042
PspFI	CCCAGC	1 cut(s)	440
PspGI	CCWGG	4 cut(s)	162, 244, 679, 1042
PspN4I	GGNNCC	5 cut(s)	199, 352, 483, 997, 1318
PspPI	GGNCC	5 cut(s)	351, 452, 995, 1317, 1728
PstI	CTGCAG	3 cut(s)	631, 862, 1174
PsuI	RGATCY	1 cut(s)	1223
PvuII	CAGCTG	2 cut(s)	1169, 1244
RsaI	GTAC	6 cut(s)	333, 611, 651, 1179, 1376, 1721
RsaNI	GTAC	6 cut(s)	332, 610, 650, 1178, 1375, 1720
RseI	CAYNNNNRTG	5 cut(s)	63, 425, 764, 860, 1763
SacI	GAGCTC	1 cut(s)	22
SapI	GCTCTTC	1 cut(s)	1640
SaqAI	TTAA	4 cut(s)	62, 639, 1599, 1665
Sau3AI	GATC	7 cut(s)	83, 555, 1078, 1105, 1223, 1426, 1627
Sau96I	GGNCC	5 cut(s)	351, 452, 995, 1317, 1728
ScaI	AGTACT	1 cut(s)	1179
SchI	GAGTC	2 cut(s)	1731, 1762
ScrFI	CCNGG	6 cut(s)	164, 246, 521, 681, 1044, 1353
SduI	GDGCHC	2 cut(s)	22, 202
SfaNI	GCATC	6 cut(s)	298, 826, 865, 1357, 1613, 1671
SfcI	CTRYAG	3 cut(s)	627, 858, 1170
SinI	GGWCC	2 cut(s)	351, 1728
SmiI	ATTTAAAT	1 cut(s)	63
SmiMI	CAYNNNNRTG	5 cut(s)	63, 425, 764, 860, 1763
SmlI	CTYRAG	1 cut(s)	1022
SmoI	CTYRAG	1 cut(s)	1022
SpeI	ACTAGT	1 cut(s)	986
SphI	GCATGC	1 cut(s)	407
Sse9I	AATT	9 cut(s)	47, 345, 367, 561, 825, 885, 912, 1259, 1438
SsiI	CCGC	5 cut(s)	195, 1061, 1082, 1572, 1580
SspMI	CTAG	3 cut(s)	179, 507, 987
SstI	GAGCTC	1 cut(s)	22
StyD4I	CCNGG	6 cut(s)	162, 244, 519, 679, 1042, 1351
StyI	CCWWGG	3 cut(s)	178, 255, 485
SwaI	ATTTAAAT	1 cut(s)	63
TaiI	ACGT	1 cut(s)	1425
TaqI	TCGA	4 cut(s)	516, 1111, 1425, 1626
TaqII	GACCGA	1 cut(s)	1745
TasI	AATT	9 cut(s)	47, 345, 367, 561, 825, 885, 912, 1259, 1438
TatI	WGTACW	2 cut(s)	331, 1177
TauI	GCSGC	2 cut(s)	1064, 1582
TfiI	GAWTC	2 cut(s)	1283, 1590
Tru1I	TTAA	4 cut(s)	62, 639, 1599, 1665
Tru9I	TTAA	4 cut(s)	62, 639, 1599, 1665
TscAI	CASTG	7 cut(s)	582, 811, 862, 867, 1216, 1750, 1770
TseFI	GTSAC	2 cut(s)	577, 1531
TseI	GCWGC	9 cut(s)	296, 510, 626, 809, 1166, 1169, 1172, 1214, 1343
Tsp45I	GTSAC	2 cut(s)	577, 1531
TspDTI	ATGAA	3 cut(s)	885, 961, 1296
TspGWI	ACGGA	1 cut(s)	123
TspRI	CASTG	7 cut(s)	582, 811, 862, 867, 1216, 1750, 1770
VpaK11BI	GGWCC	2 cut(s)	351, 1728
XapI	RAATTY	3 cut(s)	825, 885, 912
XceI	RCATGY	5 cut(s)	276, 292, 407, 435, 1618
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1582
XmaJI	CCTAGG	1 cut(s)	178
XmiI	GTMKAC	1 cut(s)	1362
XmnI	GAANNNNTTC	1 cut(s)	1649
XspI	CTAG	3 cut(s)	179, 507, 987
ZrmI	AGTACT	1 cut(s)	1179

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.