Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0006347

Physical Location & Seq

Genomic Coordinates Reverse (-)

85927649 .. 85933912

Gene Structure 6264 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00064090.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2349 bp

ATGAAGCCAGTTTTCTTCGTCATCATATCTCTCCTTCTCCTTCTCCTTCCTCTCTTGGCCTCATGCTCAAAGCAAAAGGTGTACATAGTGTATTTTGGAGAGCACGGTGGAGAGAAAGCATTGCATGAAATAGAGAACAGCCACCACTCCTATTTGCTTTCTGTGAAAGAAACTGAGGAAGATGCCAGAGCTTCACTGCTTTACAGTTACAAGCACAGCATCAATGGCTTTGCAGCAGTTCTCACTGAGGATGAAGCTTCCAAGTTATCTGAACTGGAAGAAGTTGTATCAGTGTGGCCGAGTCACCCAAAAAAGTACACTATGCACACAACAAGGTCATGGGAGTTTGTGGGGATATCGGAAGAAGAGGAAGAGGGGAATTACTGGAAGAACAACCAGATGGGAGGAGACTTCTTATCCAAAGCAAGGTCTGGCAAGAACATCATTGTTGGAGTCCTTGATAGTGGTGTATGGCCAGAATCAAAGAGCTTCAGTGACGTAGGAATGGGGCCCATTCCAAAATCATGGAAGGGAATCTGCCAATCTGGAGTTGGCTTTAATTCGTCCCACTGCAATAGGAAGCTGATAGGAGCTCGGTACTACCTCAAAGGGTTTGAGCAAAACTACGGTCCTCTTAATGCCTCAGAGGATTCTCGATCGCCACGTGACATGGACGGCCATGGAACCCACACGACCTCCACTGTGGCAGGCCGAGTCGTTCCCAATGCCTCAGCCATTGGCGGGTTCGCTCGTGGCTCTGCCTCCGGGGGTGCCCCATTGGCACACGTTGCTGTGTACAAAGTGTGTTGGGCTATACCGGGCGCATCCAAGGCTGAAGGGAACACATGCTTTGAGGAGGACATGTTTGCAGCCATAGATGATGCCATAGCAGACGGTGTGGATGTGCTGAGCATATCAATTGGCACAACTCGACCTATCAATTTCACCGAGGACGGTATTGCTCTCGGAGCATTGCATGCCGCCAAGAAGAACATTGTGGTGGCATGTAGTGCTGGCAACTCAGGCCCTGCCCCATCTACATTATCAAACCCAGCTCCATGGATCATCACGGTTGGTGCCAGCAGCTTGGACCGAACTTTTGTGTCACCCGTTGTGCTTGGAAATGGTTTGAGCATTGAGGGAGAAACAGTGACCCCAAGCAAGCTGGAAGAGAATAAGATGTACCCTTTAGTATATGCAGGGGATGTTGTTAACCCTGGTGTAGCCCAAAATTTGACAGGACAATGCCTCGCAGGCTCTCTTTCACCTGGCAAAGTTAAGGGAAAGATAGTGTTGTGCAGGAGAGGTGCTGGGATGAGAGTTTCAAAAGGCATGGAAGTGAAAAGGGCGGGTGGTGTAGGTTTTATCTTAGGAAACAGCAAAGCAAATGGCGTTGAAATCTCATGTGATGCTCATGTTCTTCCTGCCACTGCAATGACATACAGTAATGCCAATAGAATCATGGAATACATCAACTCTACCAAAAATCCAAAGGCAACTATTATACCAGCAAGGACAGTGTTGAATACAAAGCCAGCTCCATACATGACTGCTTTCACTAGTAGAGGCCCAAGTACCATTGATCCTAACATTCTCAAGCCTGATATCACTGCTCCTGGACTGAATATATTGGCAGCATGGACTGAGGCTGATCCCCCTACCAAGTTGTCAATGGATCACCGAGTGGCTCAGTATAACATTGAGTCTGGAACTTCAATGTCTTGCCCTCACATTGCTGCGGCGGCAGCACTTCTTAAAGCCATCCACCCTACTTGGAGCAGTGCTGCCATCAAATCTGCTCTAATGACCACAGCGGGGATAAGAAACAACCTAGATAAGCAATTGAACGATGAATCGGGCAATGCTGCAACTCCCTTCGCATATGGTGCTGGCCACTTCCGACCCACCAAGGCAGCAGACCCTGGCCTTGTGTACGATGCTTCATACAAAGACTATCTTCTCTACTTCTGCAGTACCGGAGTCAAGAATTTCGATCCAAAATTCAAGTGTCCAAGATCACCACCAACACCAATCAACCTGAACTATCCTTCCATTGCAATCCCCAAACTGAATGGCACCATCACGATTAAGAGGACAGTCACAAATGTTGGTGACCCCAAAAGCGTTTACTTCTTTACCTCCAAACCGCCTCTTGGAATCTCGGTTAAGGCCTCTCCGAGTATATTGTTCTTTGATCACGTTGGCCAGAGGAAGAGCTTCACCATTACTGTCAAAGCAAGGACAGAGATGCTTAACGAGAATCAGTTGAAAGATGAGTATGCATTTGGGTGGTACACGTGGACTGATGGACCTCATATTGTAAGGAGTCCAATTGCAGTCTCTCTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0016787 GO:0017171 GO:0019538 GO:0043170 GO:0044238 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8

Protein Analysis

782

Amino Acids

83.85

Weight (kDa)

8.58

Isoelectric Point (pI)

36.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	27 - 103	1.6e-19	Peptidase inhibitor I9
Peptidase_S8	PF00082	145 - 631	3.2e-40	Subtilase family
PA	PF02225	395 - 487	1.5e-11	PA domain
fn3_6	PF17766	679 - 780	2.5e-29	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006347)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G45650 AT5G45650
fragaria_vesca	FvH4_3g06570 FvH4_3g06570 FvH4_3g06570 FvH4_3g06570 FvH4_3g06570 FvH4_3g06570 FvH4_3g33200
malus_domestica	MD00G1093400.v1.1 MD10G1270100.v1.1
prunus_persica	Prupe.4G071500_v2.0.a1
pyrus_communis	pycom05g27040 pycom10g22620
rosa_chinensis	RchiOBHm_Chr5g0012581
rosa_laevigata	RLG00000031926
rosa_multiflora	Rmu_co8464651.1_g000001 Rmu_sc0002639.1_g000013 Rmu_sc0003577.1_g000010
rosa_roxburghii	Rroxscaffold_1G00064090
rosa_rugosa	Rorug05G0002700 Rorug05G0002700 Rorug05G0002700
rosa_samantha	Rh5AG096500 Rh5CG105600 Rh5DG092200
rosa_wichuraiana	Rw5G008450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	3 cut(s)	770, 1076, 2075
AciI	CCGC	7 cut(s)	741, 981, 1349, 1739, 1742, 1814, 2147
AclWI	GGATC	5 cut(s)	1070, 1577, 1646, 1683, 1988
AcoI	YGGCCR	5 cut(s)	296, 473, 676, 1891, 2203
AcsI	RAATTY	3 cut(s)	1231, 1987, 2000
AcuI	CTGAAG	2 cut(s)	475, 855
AcvI	CACGTG	2 cut(s)	665, 2298
AdeI	CACNNNGTG	1 cut(s)	1684
AfaI	GTAC	9 cut(s)	83, 317, 599, 797, 1184, 1576, 1934, 1975, 2294
AfiI	CCNNNNNNNGG	4 cut(s)	426, 741, 1815, 2153
AflIII	ACRYGT	3 cut(s)	784, 861, 2295
AgsI	TTSAA	7 cut(s)	1326, 1397, 1525, 1716, 1846, 2005, 2269
AhlI	ACTAGT	1 cut(s)	1559
AjnI	CCWGG	4 cut(s)	1216, 1267, 1615, 1921
AloI	GAACNNNNNNTCC	2 cut(s)	2033, 2065
Alw21I	GWGCWC	2 cut(s)	105, 595
Alw26I	GTCTC	2 cut(s)	402, 2344
AlwI	GGATC	5 cut(s)	1070, 1577, 1646, 1683, 1988
AlwNI	CAGNNNCTG	2 cut(s)	1028, 1922
ApaI	GGGCCC	1 cut(s)	513
ApeKI	GCWGC	9 cut(s)	233, 869, 1083, 1634, 1736, 1745, 1784, 1865, 1913
ApoI	RAATTY	3 cut(s)	1231, 1987, 2000
ArsI	GACNNNNNNTTYG	4 cut(s)	613, 645, 1082, 1114
Asp700I	GAANNNNTTC	1 cut(s)	2216
AspLEI	GCGC	1 cut(s)	824
AspS9I	GGNCC	7 cut(s)	509, 510, 629, 1025, 1090, 1568, 2309
AsuC2I	CCSGG	2 cut(s)	766, 819
AsuHPI	GGTGA	8 cut(s)	296, 937, 1098, 1257, 1670, 2010, 2123, 2212
AvaII	GGWCC	3 cut(s)	629, 1090, 2309
BaeGI	GKGCMC	2 cut(s)	513, 775
BalI	TGGCCA	3 cut(s)	475, 1893, 2205
BanI	GGYRCC	3 cut(s)	770, 1076, 2075
BanII	GRGCYC	2 cut(s)	513, 595
BauI	CACGAG	1 cut(s)	750
BbrPI	CACGTG	2 cut(s)	665, 2298
Bbv12I	GWGCWC	2 cut(s)	105, 595
BbvCI	CCTCAGC	1 cut(s)	730
BbvI	GCAGC	9 cut(s)	245, 881, 1095, 1646, 1723, 1757, 1771, 1852, 1925
BccI	CCATC	6 cut(s)	394, 1042, 1769, 1796, 2087, 2300
BceAI	ACGGC	1 cut(s)	691
BciT130I	CCWGG	4 cut(s)	1218, 1269, 1617, 1923
BclI	TGATCA	1 cut(s)	2194
BcnI	CCSGG	2 cut(s)	766, 819
BcoDI	GTCTC	2 cut(s)	402, 2344
BcuI	ACTAGT	1 cut(s)	1559
BfaI	CTAG	2 cut(s)	1560, 1832
BfmI	CTRYAG	1 cut(s)	1969
BglI	GCCNNNNNGGC	2 cut(s)	779, 1254
BlpI	GCTNAGC	1 cut(s)	908
Bme1390I	CCNGG	6 cut(s)	766, 819, 1218, 1269, 1617, 1923
Bme18I	GGWCC	3 cut(s)	629, 1090, 2309
BmgT120I	GGNCC	7 cut(s)	509, 510, 629, 1025, 1090, 1568, 2309
BmiI	GGNNCC	6 cut(s)	510, 511, 685, 772, 1078, 2077
BmrFI	CCNGG	6 cut(s)	766, 819, 1218, 1269, 1617, 1923
BmsI	GCATC	7 cut(s)	172, 228, 833, 871, 1399, 1927, 2238
BpmI	CTGGAG	1 cut(s)	567
Bpu10I	CCTNAGC	1 cut(s)	730
Bpu1102I	GCTNAGC	1 cut(s)	908
BpuEI	CTTGAG	1 cut(s)	1580
BpuMI	CCSGG	2 cut(s)	766, 819
BsaAI	YACGTR	2 cut(s)	665, 2298
BsaJI	CCNNGG	8 cut(s)	679, 765, 828, 948, 1058, 1216, 1908, 1921
BsaWI	WCCGGW	1 cut(s)	1976
BsaXI	ACNNNNNCTCC	6 cut(s)	680, 710, 848, 878, 1134, 1164
Bsc4I	CCNNNNNNNGG	4 cut(s)	426, 741, 1815, 2153
Bse1I	ACTGG	3 cut(s)	8, 279, 389
Bse3DI	GCAATG	6 cut(s)	119, 971, 1440, 1731, 1867, 2052
BseBI	CCWGG	4 cut(s)	1218, 1269, 1617, 1923
BseDI	CCNNGG	8 cut(s)	679, 765, 828, 948, 1058, 1216, 1908, 1921
BseGI	GGATG	6 cut(s)	256, 824, 907, 1210, 1320, 1761
BseLI	CCNNNNNNNGG	4 cut(s)	426, 741, 1815, 2153
BseMI	GCAATG	6 cut(s)	119, 971, 1440, 1731, 1867, 2052
BseMII	CTCAG	8 cut(s)	165, 237, 657, 744, 899, 1035, 1635, 1703
BseNI	ACTGG	3 cut(s)	8, 279, 389
BseRI	GAGGAG	2 cut(s)	420, 869
BseSI	GKGCMC	2 cut(s)	513, 775
BseXI	GCAGC	9 cut(s)	245, 881, 1095, 1646, 1723, 1757, 1771, 1852, 1925
BseYI	CCCAGC	2 cut(s)	1051, 1310
BsgI	GTGCAG	1 cut(s)	1318
Bsh1285I	CGRYCG	1 cut(s)	659
BshNI	GGYRCC	3 cut(s)	770, 1076, 2075
BsiEI	CGRYCG	1 cut(s)	659
BsiHKAI	GWGCWC	2 cut(s)	105, 595
BsiSI	CCGG	3 cut(s)	765, 818, 1977
BslFI	GGGAC	1 cut(s)	550
BslI	CCNNNNNNNGG	4 cut(s)	426, 741, 1815, 2153
BsmAI	GTCTC	2 cut(s)	402, 2344
BsmFI	GGGAC	1 cut(s)	550
Bsp120I	GGGCCC	1 cut(s)	509
Bsp1286I	GDGCHC	4 cut(s)	105, 513, 595, 775
Bsp1407I	TGTACA	2 cut(s)	81, 795
Bsp143I	GATC	8 cut(s)	656, 1062, 1582, 1651, 1675, 1993, 2015, 2194
Bsp1720I	GCTNAGC	1 cut(s)	908
Bsp19I	CCATGG	2 cut(s)	679, 1058
BspACI	CCGC	7 cut(s)	741, 981, 1349, 1739, 1742, 1814, 2147
BspCNI	CTCAG	8 cut(s)	166, 238, 656, 743, 900, 1034, 1636, 1702
BspLI	GGNNCC	6 cut(s)	510, 511, 685, 772, 1078, 2077
BspMAI	CTGCAG	1 cut(s)	1973
BspPI	GGATC	5 cut(s)	1070, 1577, 1646, 1683, 1988
BspQI	GCTCTTC	1 cut(s)	2207
BspT107I	GGYRCC	3 cut(s)	770, 1076, 2075
BsrDI	GCAATG	6 cut(s)	119, 971, 1440, 1731, 1867, 2052
BsrGI	TGTACA	2 cut(s)	81, 795
BsrI	ACTGG	3 cut(s)	8, 279, 389
BssECI	CCNNGG	8 cut(s)	679, 765, 828, 948, 1058, 1216, 1908, 1921
BssMI	GATC	8 cut(s)	656, 1062, 1582, 1651, 1675, 1993, 2015, 2194
BssSI	CACGAG	1 cut(s)	750
BssT1I	CCWWGG	4 cut(s)	679, 828, 1058, 1908
Bst2BI	CACGAG	1 cut(s)	750
Bst2UI	CCWGG	4 cut(s)	1218, 1269, 1617, 1923
Bst6I	CTCTTC	4 cut(s)	360, 366, 1164, 2207
BstAPI	GCANNNNNTGC	4 cut(s)	788, 977, 1010, 1886
BstAUI	TGTACA	2 cut(s)	81, 795
BstBAI	YACGTR	2 cut(s)	665, 2298
BstC8I	GCNNGC	8 cut(s)	709, 978, 1015, 1081, 1163, 1255, 1536, 1891
BstDEI	CTNAG	9 cut(s)	174, 246, 643, 730, 908, 1021, 1369, 1644, 1689
BstDSI	CCRYGG	2 cut(s)	679, 1058
BstEII	GGTNACC	1 cut(s)	2111
BstF5I	GGATG	6 cut(s)	256, 824, 907, 1210, 1320, 1761
BstHHI	GCGC	1 cut(s)	824
BstKTI	GATC	8 cut(s)	659, 1065, 1585, 1654, 1678, 1996, 2018, 2197
BstMAI	GTCTC	2 cut(s)	402, 2344
BstMBI	GATC	8 cut(s)	656, 1062, 1582, 1651, 1675, 1993, 2015, 2194
BstMCI	CGRYCG	1 cut(s)	659
BstNI	CCWGG	4 cut(s)	1218, 1269, 1617, 1923
BstNSI	RCATGY	4 cut(s)	849, 865, 980, 1008
BstPI	GGTNACC	1 cut(s)	2111
BstSCI	CCNGG	6 cut(s)	764, 817, 1216, 1267, 1615, 1921
BstSFI	CTRYAG	1 cut(s)	1969
BstSLI	GKGCMC	2 cut(s)	513, 775
BstV1I	GCAGC	9 cut(s)	245, 881, 1095, 1646, 1723, 1757, 1771, 1852, 1925
BstXI	CCANNNNNNTGG	3 cut(s)	525, 1059, 1087
BtgI	CCRYGG	2 cut(s)	679, 1058
BtsCI	GGATG	6 cut(s)	256, 824, 907, 1210, 1320, 1761
BtsI	GCAGTG	5 cut(s)	194, 568, 1428, 1608, 1786
Cac8I	GCNNGC	8 cut(s)	709, 978, 1015, 1081, 1163, 1255, 1536, 1891
CaiI	CAGNNNCTG	2 cut(s)	1028, 1922
CfoI	GCGC	1 cut(s)	824
Cfr13I	GGNCC	7 cut(s)	509, 510, 629, 1025, 1090, 1568, 2309
Csp6I	GTAC	9 cut(s)	82, 316, 598, 796, 1183, 1575, 1933, 1974, 2293
CspCI	CAANNNNNGTGG	2 cut(s)	1754, 1789
CviQI	GTAC	9 cut(s)	82, 316, 598, 796, 1183, 1575, 1933, 1974, 2293
DdeI	CTNAG	9 cut(s)	174, 246, 643, 730, 908, 1021, 1369, 1644, 1689
DpnI	GATC	8 cut(s)	658, 1064, 1584, 1653, 1677, 1995, 2017, 2196
DpnII	GATC	8 cut(s)	656, 1062, 1582, 1651, 1675, 1993, 2015, 2194
DraIII	CACNNNGTG	1 cut(s)	1684
EaeI	YGGCCR	5 cut(s)	296, 473, 676, 1891, 2203
Eam1104I	CTCTTC	4 cut(s)	360, 366, 1164, 2207
EarI	CTCTTC	4 cut(s)	360, 366, 1164, 2207
Ecl136II	GAGCTC	1 cut(s)	593
Eco130I	CCWWGG	4 cut(s)	679, 828, 1058, 1908
Eco147I	AGGCCT	1 cut(s)	2171
Eco24I	GRGCYC	2 cut(s)	513, 595
Eco32I	GATATC	2 cut(s)	357, 1606
Eco47I	GGWCC	3 cut(s)	629, 1090, 2309
Eco53kI	GAGCTC	1 cut(s)	593
Eco57I	CTGAAG	2 cut(s)	475, 855
Eco72I	CACGTG	2 cut(s)	665, 2298
Eco91I	GGTNACC	1 cut(s)	2111
EcoICRI	GAGCTC	1 cut(s)	593
EcoO109I	RGGNCCY	2 cut(s)	509, 1025
EcoO65I	GGTNACC	1 cut(s)	2111
EcoRII	CCWGG	4 cut(s)	1216, 1267, 1615, 1921
EcoRV	GATATC	2 cut(s)	357, 1606
EcoT14I	CCWWGG	4 cut(s)	679, 828, 1058, 1908
EcoT22I	ATGCAT	1 cut(s)	2284
EcoT38I	GRGCYC	2 cut(s)	513, 595
ErhI	CCWWGG	4 cut(s)	679, 828, 1058, 1908
FalI	AAGNNNNNCTT	2 cut(s)	1941, 1973
FaqI	GGGAC	1 cut(s)	550
FauI	CCCGC	3 cut(s)	734, 1342, 1807
FauNDI	CATATG	1 cut(s)	1882
FbaI	TGATCA	1 cut(s)	2194
FokI	GGATG	6 cut(s)	263, 811, 914, 1217, 1327, 1748
FriOI	GRGCYC	2 cut(s)	513, 595
FspBI	CTAG	2 cut(s)	1560, 1832
GlaI	GCGC	1 cut(s)	823
GsaI	CCCAGC	2 cut(s)	1055, 1314
GsuI	CTGGAG	1 cut(s)	567
HapII	CCGG	3 cut(s)	765, 818, 1977
HhaI	GCGC	1 cut(s)	824
Hin6I	GCGC	1 cut(s)	822
HinP1I	GCGC	1 cut(s)	822
HincII	GTYRAC	1 cut(s)	1213
HindII	GTYRAC	1 cut(s)	1213
HindIII	AAGCTT	1 cut(s)	255
HpaI	GTTAAC	1 cut(s)	1213
HpaII	CCGG	3 cut(s)	765, 818, 1977
HphI	GGTGA	8 cut(s)	296, 937, 1098, 1257, 1670, 2010, 2123, 2212
Hpy166II	GTNNAC	8 cut(s)	82, 318, 796, 1213, 1933, 2128, 2295, 2301
Hpy188I	TCNGA	6 cut(s)	271, 361, 646, 968, 1901, 2178
Hpy188III	TCNNGA	5 cut(s)	546, 654, 1707, 1984, 2083
Hpy8I	GTNNAC	8 cut(s)	82, 318, 796, 1213, 1933, 2128, 2295, 2301
HpyAV	CCTTC	7 cut(s)	44, 50, 56, 523, 830, 1885, 2058
HpyCH4IV	ACGT	5 cut(s)	498, 664, 786, 2199, 2297
HpyF3I	CTNAG	9 cut(s)	174, 246, 643, 730, 908, 1021, 1369, 1644, 1689
HpySE526I	ACGT	5 cut(s)	498, 664, 786, 2199, 2297
HspAI	GCGC	1 cut(s)	822
Ksp22I	TGATCA	1 cut(s)	2194
KspAI	GTTAAC	1 cut(s)	1213
Kzo9I	GATC	8 cut(s)	656, 1062, 1582, 1651, 1675, 1993, 2015, 2194
LguI	GCTCTTC	1 cut(s)	2207
LmnI	GCTCC	6 cut(s)	590, 968, 1060, 1543, 1618, 1776
Lsp1109I	GCAGC	9 cut(s)	245, 881, 1095, 1646, 1723, 1757, 1771, 1852, 1925
LweI	GCATC	7 cut(s)	172, 228, 833, 871, 1399, 1927, 2238
MaeI	CTAG	2 cut(s)	1560, 1832
MaeII	ACGT	5 cut(s)	498, 664, 786, 2199, 2297
MaeIII	GTNAC	8 cut(s)	206, 302, 494, 665, 1104, 1150, 2098, 2111
MalI	GATC	8 cut(s)	658, 1064, 1584, 1653, 1677, 1995, 2017, 2196
MboI	GATC	8 cut(s)	656, 1062, 1582, 1651, 1675, 1993, 2015, 2194
MfeI	CAATTG	3 cut(s)	918, 1841, 2331
MhlI	GDGCHC	4 cut(s)	105, 513, 595, 775
MlsI	TGGCCA	3 cut(s)	475, 1893, 2205
MluCI	AATT	9 cut(s)	379, 559, 918, 940, 1231, 1841, 1987, 2000, 2331
MluNI	TGGCCA	3 cut(s)	475, 1893, 2205
MlyI	GAGTC	6 cut(s)	310, 462, 723, 1712, 1989, 2335
MmeI	TCCRAC	2 cut(s)	430, 1924
Mox20I	TGGCCA	3 cut(s)	475, 1893, 2205
Mph1103I	ATGCAT	1 cut(s)	2284
MroXI	GAANNNNTTC	1 cut(s)	2216
MscI	TGGCCA	3 cut(s)	475, 1893, 2205
MseI	TTAA	8 cut(s)	558, 636, 1212, 1278, 1755, 2088, 2166, 2253
MslI	CAYNNNNRTG	4 cut(s)	998, 1337, 1433, 2287
Msp20I	TGGCCA	3 cut(s)	475, 1893, 2205
MspA1I	CMGCKG	1 cut(s)	1814
MspI	CCGG	3 cut(s)	765, 818, 1977
MspR9I	CCNGG	6 cut(s)	766, 819, 1218, 1269, 1617, 1923
MunI	CAATTG	3 cut(s)	918, 1841, 2331
MvaI	CCWGG	4 cut(s)	1218, 1269, 1617, 1923
NciI	CCSGG	2 cut(s)	766, 819
NcoI	CCATGG	2 cut(s)	679, 1058
NdeI	CATATG	1 cut(s)	1882
NdeII	GATC	8 cut(s)	656, 1062, 1582, 1651, 1675, 1993, 2015, 2194
NlaIV	GGNNCC	6 cut(s)	510, 511, 685, 772, 1078, 2077
NmeAIII	GCCGAG	2 cut(s)	324, 737
NmuCI	GTSAC	7 cut(s)	302, 494, 665, 1104, 1150, 2098, 2111
NsiI	ATGCAT	1 cut(s)	2284
NspI	RCATGY	4 cut(s)	849, 865, 980, 1008
PaeI	GCATGC	1 cut(s)	980
PceI	AGGCCT	1 cut(s)	2171
PciI	ACATGT	1 cut(s)	861
PciSI	GCTCTTC	1 cut(s)	2207
PcsI	WCGNNNNNNNCGW	1 cut(s)	661
PdmI	GAANNNNTTC	1 cut(s)	2216
PfeI	GAWTC	7 cut(s)	479, 534, 650, 1458, 1853, 2157, 2260
PfoI	TCCNGGA	1 cut(s)	1615
Ple19I	CGATCG	1 cut(s)	659
PleI	GAGTC	6 cut(s)	309, 461, 722, 1711, 1988, 2334
PmaCI	CACGTG	2 cut(s)	665, 2298
PmlI	CACGTG	2 cut(s)	665, 2298
PpsI	GAGTC	6 cut(s)	309, 461, 722, 1711, 1988, 2334
Ppu21I	YACGTR	2 cut(s)	665, 2298
PscI	ACATGT	1 cut(s)	861
Psp124BI	GAGCTC	1 cut(s)	595
Psp6I	CCWGG	4 cut(s)	1216, 1267, 1615, 1921
PspCI	CACGTG	2 cut(s)	665, 2298
PspEI	GGTNACC	1 cut(s)	2111
PspFI	CCCAGC	2 cut(s)	1051, 1310
PspGI	CCWGG	4 cut(s)	1216, 1267, 1615, 1921
PspN4I	GGNNCC	6 cut(s)	510, 511, 685, 772, 1078, 2077
PspOMI	GGGCCC	1 cut(s)	509
PspPI	GGNCC	7 cut(s)	509, 510, 629, 1025, 1090, 1568, 2309
PstI	CTGCAG	1 cut(s)	1973
PstNI	CAGNNNCTG	2 cut(s)	1028, 1922
PvuI	CGATCG	1 cut(s)	659
RsaI	GTAC	9 cut(s)	83, 317, 599, 797, 1184, 1576, 1934, 1975, 2294
RsaNI	GTAC	9 cut(s)	82, 316, 598, 796, 1183, 1575, 1933, 1974, 2293
RseI	CAYNNNNRTG	4 cut(s)	998, 1337, 1433, 2287
SacI	GAGCTC	1 cut(s)	595
SapI	GCTCTTC	1 cut(s)	2207
SaqAI	TTAA	8 cut(s)	558, 636, 1212, 1278, 1755, 2088, 2166, 2253
Sau3AI	GATC	8 cut(s)	656, 1062, 1582, 1651, 1675, 1993, 2015, 2194
Sau96I	GGNCC	7 cut(s)	509, 510, 629, 1025, 1090, 1568, 2309
SchI	GAGTC	6 cut(s)	310, 462, 723, 1712, 1989, 2335
ScrFI	CCNGG	6 cut(s)	766, 819, 1218, 1269, 1617, 1923
SduI	GDGCHC	4 cut(s)	105, 513, 595, 775
SfaNI	GCATC	7 cut(s)	172, 228, 833, 871, 1399, 1927, 2238
SfcI	CTRYAG	1 cut(s)	1969
SinI	GGWCC	3 cut(s)	629, 1090, 2309
SmiMI	CAYNNNNRTG	4 cut(s)	998, 1337, 1433, 2287
SmlI	CTYRAG	1 cut(s)	1595
SmoI	CTYRAG	1 cut(s)	1595
SpeI	ACTAGT	1 cut(s)	1559
SphI	GCATGC	1 cut(s)	980
Sse9I	AATT	9 cut(s)	379, 559, 918, 940, 1231, 1841, 1987, 2000, 2331
SseBI	AGGCCT	1 cut(s)	2171
SsiI	CCGC	7 cut(s)	741, 981, 1349, 1739, 1742, 1814, 2147
SspMI	CTAG	2 cut(s)	1560, 1832
SstI	GAGCTC	1 cut(s)	595
StuI	AGGCCT	1 cut(s)	2171
StyD4I	CCNGG	6 cut(s)	764, 817, 1216, 1267, 1615, 1921
StyI	CCWWGG	4 cut(s)	679, 828, 1058, 1908
TaiI	ACGT	5 cut(s)	501, 667, 789, 2202, 2300
TaqI	TCGA	3 cut(s)	655, 931, 1992
TaqII	GACCGA	1 cut(s)	1107
TasI	AATT	9 cut(s)	379, 559, 918, 940, 1231, 1841, 1987, 2000, 2331
TatI	WGTACW	3 cut(s)	81, 315, 795
TauI	GCSGC	3 cut(s)	983, 1742, 1745
TfiI	GAWTC	7 cut(s)	479, 534, 650, 1458, 1853, 2157, 2260
Tru1I	TTAA	8 cut(s)	558, 636, 1212, 1278, 1755, 2088, 2166, 2253
Tru9I	TTAA	8 cut(s)	558, 636, 1212, 1278, 1755, 2088, 2166, 2253
TseFI	GTSAC	7 cut(s)	302, 494, 665, 1104, 1150, 2098, 2111
TseI	GCWGC	9 cut(s)	233, 869, 1083, 1634, 1736, 1745, 1784, 1865, 1913
Tsp45I	GTSAC	7 cut(s)	302, 494, 665, 1104, 1150, 2098, 2111
TspDTI	ATGAA	5 cut(s)	17, 141, 267, 1866, 1932
VpaK11BI	GGWCC	3 cut(s)	629, 1090, 2309
XapI	RAATTY	3 cut(s)	1231, 1987, 2000
XceI	RCATGY	4 cut(s)	849, 865, 980, 1008
XcmI	CCANNNNNNNNNTGG	3 cut(s)	548, 1459, 1669
XmnI	GAANNNNTTC	1 cut(s)	2216
XspI	CTAG	2 cut(s)	1560, 1832
Zsp2I	ATGCAT	1 cut(s)	2284

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.