Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0005816

Physical Location & Seq

Genomic Coordinates Forward (+)

23132699 .. 23151567

Gene Structure 18869 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1110700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1446 bp

ATGGTTGACGGCGGCGCAAGCGGCAACAGAGGCCATAACAACAACAACCACAGCCACGGCGGTGGTCATAGTAACAAGCCGGAGTGGCTCCAACAGTACGATCTGATAGGGAAGATCGGGGAAGGCACCTACGGCCTGGTGTTTCTTGCAAGGACCAAGTCTCCTCCGTCCAACCGCGGAAAATCCATCGCCATCAAGAAATTCAAGCAGTCCAAGGACGGTGATGGCGTCTCCCCCACTGCCATCCGTGAAATCATGTTGCTGCGGGAAATATCGCACGAGAATGTGGTGAAGCTCGTGAACGTTCATATCAACCACACAGACATGTCCCTCTATCTGGCTTTCGATTACGCAGAACATGACCTTTATGAAATCATTAGACATCACAGAGACAAAGTTAATCACGCAATCAATTATTACACTATTAAGACATTGCTCTGGCAGCTGCTCAATGGACTCAATTATCTCCATAGTAACTGGATCATTCATCGTGATTTAAAGCCATCTAATATCTTGGTTATGGGCGACAAAGAGGAGCAAGGAGTTGTGAAAATTGCGGATTTTGGACTTGCAAGGATATATCAGGCTCCCTTAAAGTCGTTGTCTGAAAATGGGGTTGTGGTAACCATTTGGTACCGTGCACCGGAATTGCTCCTTGGGGCTAAGCACTATACAAGTGCTGTTGATATGTGGGCTGTCGGATGCATCTTTGCTGAGCTTTTGACTTTGAAGCCATTATTTCAAGGCGCAGAAGTCAAAGCTACACCAAACCCTTTTCAGCTTGATCAACTTGACAAGATATTCAAGGTCTTGGGTCATCCTACACTAGAAAAGTGGCCAGCACTTGCGAACCTTCCACAATGGCAACAAGATATACAACATATTCAAGGCCATAAATATGATAATACTGCGTTGTATAGTGTAGTTAACCTCTCTCCAAAAAGTCCTGCATATGACCTGCTATCTAAGATGCTTGAATATGATCCTCGAAAGCGTATAACAGCTGCACAAGCATTAGAGCATGAGTATTTTCGTTTTGAACCTCTACCAGGACGCAATGCTTTGGTACCTACCCAAGGAGAGAAAGTAGTGAATTATCCTACTCGTCCGGTAGACACAACCACAGATTTTGAAGGGACAACTAGCATTCAACCTTCACAACCGGTATCAACTGGAAATGCACTATCTGGAAACATGTCAGCTGCTCATGGAGGGATGAACAGATCCGTGCCCAGGCCGATGCTTAACCTGCAAAGAATGCAGCCACAGGGCATGACTGCTTATAATCTTGCATCTCAGGCAGGGATGGGTGGTGCAATGAATCCTGCCGGCATGCCAATGCAGCGTGGTGTAGCACAGGCCCATCACCAACAGCAGTTGAGAAGGAAAGATAATGGAATGGGGATGACGGGATACCCTCCACAGCAGAAATCCAGACGTTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004693 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007346 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016592 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0031974 GO:0031981 GO:0032991 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0050789 GO:0050794 GO:0051726 GO:0065007 GO:0070013 GO:0071704 GO:0097472 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

482

Amino Acids

53.79

Weight (kDa)

9.33

Isoelectric Point (pI)

38.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	33 - 344	4.8e-66	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	36 - 243	7.5e-34	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005816)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G63610
fragaria_vesca	FvH4_1g25340 FvH4_1g25340 FvH4_1g25350
malus_domestica	MD00G1110700.v1.1 MD15G1137900.v1.1
prunus_persica	Prupe.4G280700_v2.0.a1
pyrus_communis	pycom1146g00040
rosa_chinensis	RchiOBHm_Chr2g0123381 RchiOBHm_Chr2g0123391
rosa_laevigata	RLG00000018746
rosa_multiflora	Rmu_sc0004330.1_g000003 Rmu_sc0024478.1_g000001 Rmu_sc0041165.1_g000001 Rmu_ssc0000345.1_g000001
rosa_roxburghii	Rroxscaffold_2G00120740 Rroxscaffold_2G00120750
rosa_rugosa	Rorug02G0244900 Rorug02G0244900 Rorug02G0245000
rosa_samantha	Rh2AG303700 Rh2BG311800 Rh2CG290300 Rh2CG290400 Rh2DG327800 Rh2DG327900
rosa_wichuraiana	Rw2G024310 Rw2G024320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1284
Acc36I	ACCTGC	2 cut(s)	966, 1257
Acc65I	GGTACC	2 cut(s)	633, 1066
AccB1I	GGYRCC	3 cut(s)	125, 633, 1066
AccI	GTMKAC	1 cut(s)	1113
AccII	CGCG	1 cut(s)	177
AciI	CCGC	7 cut(s)	12, 21, 60, 175, 177, 265, 557
AclI	AACGTT	1 cut(s)	303
AclWI	GGATC	3 cut(s)	488, 977, 1218
AcoI	YGGCCR	1 cut(s)	836
AcsI	RAATTY	1 cut(s)	200
AcyI	GRCGYC	1 cut(s)	228
AfaI	GTAC	3 cut(s)	98, 635, 1068
AfiI	CCNNNNNNNGG	5 cut(s)	337, 643, 1049, 1076, 1233
AflIII	ACRYGT	2 cut(s)	324, 1194
AgeI	ACCGGT	1 cut(s)	1162
AgsI	TTSAA	9 cut(s)	205, 730, 743, 805, 887, 977, 1040, 1133, 1151
AjnI	CCWGG	3 cut(s)	135, 1048, 1232
AjuI	GAANNNNNNNTTGG	2 cut(s)	639, 671
AleI	CACNNNNGTG	1 cut(s)	60
AluBI	AGCT	7 cut(s)	295, 445, 718, 761, 781, 1004, 1202
AluI	AGCT	7 cut(s)	295, 445, 718, 761, 781, 1004, 1202
Alw21I	GWGCWC	1 cut(s)	643
Alw26I	GTCTC	3 cut(s)	165, 235, 384
Alw44I	GTGCAC	1 cut(s)	639
AlwI	GGATC	3 cut(s)	488, 977, 1218
AoxI	GGCC	6 cut(s)	31, 133, 836, 889, 1235, 1359
ApaLI	GTGCAC	1 cut(s)	639
ApeKI	GCWGC	7 cut(s)	262, 442, 445, 1004, 1202, 1261, 1342
ApoI	RAATTY	1 cut(s)	200
AsiGI	ACCGGT	1 cut(s)	1162
Asp718I	GGTACC	2 cut(s)	633, 1066
AspLEI	GCGC	2 cut(s)	17, 749
AspS9I	GGNCC	2 cut(s)	153, 1360
AsuHPI	GGTGA	3 cut(s)	233, 301, 1358
AvaII	GGWCC	1 cut(s)	153
BaeGI	GKGCMC	2 cut(s)	643, 1233
BalI	TGGCCA	1 cut(s)	838
BanI	GGYRCC	3 cut(s)	125, 633, 1066
BauI	CACGAG	2 cut(s)	278, 296
Bbv12I	GWGCWC	1 cut(s)	643
BbvI	GCAGC	7 cut(s)	249, 432, 454, 991, 1189, 1273, 1354
BccI	CCATC	7 cut(s)	194, 200, 218, 251, 511, 1300, 1371
BceAI	ACGGC	3 cut(s)	25, 73, 148
BciT130I	CCWGG	3 cut(s)	137, 1050, 1234
BciVI	GTATCC	1 cut(s)	1406
BclI	TGATCA	1 cut(s)	784
BcoDI	GTCTC	3 cut(s)	165, 235, 384
BfaI	CTAG	2 cut(s)	827, 1143
BfuAI	ACCTGC	2 cut(s)	966, 1257
BfuI	GTATCC	1 cut(s)	1406
BglI	GCCNNNNNGGC	1 cut(s)	85
BisI	GCNGC	9 cut(s)	13, 22, 263, 443, 446, 1005, 1203, 1262, 1343
BlpI	GCTNAGC	2 cut(s)	663, 714
BlsI	GCNGC	9 cut(s)	14, 23, 264, 444, 447, 1006, 1204, 1263, 1344
Bme1390I	CCNGG	3 cut(s)	137, 1050, 1234
Bme18I	GGWCC	1 cut(s)	153
BmgT120I	GGNCC	2 cut(s)	153, 1360
BmiI	GGNNCC	5 cut(s)	89, 127, 588, 635, 1068
BmrFI	CCNGG	3 cut(s)	137, 1050, 1234
BmsI	GCATC	5 cut(s)	692, 714, 960, 1230, 1301
Bpu1102I	GCTNAGC	2 cut(s)	663, 714
BsaHI	GRCGYC	1 cut(s)	228
BsaJI	CCNNGG	6 cut(s)	55, 175, 213, 655, 1075, 1232
BsaWI	WCCGGW	3 cut(s)	643, 1108, 1162
BsaXI	ACNNNNNCTCC	4 cut(s)	145, 175, 1071, 1101
Bsc4I	CCNNNNNNNGG	5 cut(s)	337, 643, 1049, 1076, 1233
Bse118I	RCCGGY	2 cut(s)	1162, 1328
Bse1I	ACTGG	2 cut(s)	482, 1177
Bse3DI	GCAATG	3 cut(s)	431, 1063, 1323
BseBI	CCWGG	3 cut(s)	137, 1050, 1234
BseDI	CCNNGG	6 cut(s)	55, 175, 213, 655, 1075, 1232
BseGI	GGATG	6 cut(s)	243, 707, 817, 1221, 1311, 1410
BseLI	CCNNNNNNNGG	5 cut(s)	337, 643, 1049, 1076, 1233
BseMI	GCAATG	3 cut(s)	431, 1063, 1323
BseMII	CTCAG	2 cut(s)	705, 1310
BseNI	ACTGG	2 cut(s)	482, 1177
BseRI	GAGGAG	2 cut(s)	153, 548
BseSI	GKGCMC	2 cut(s)	643, 1233
BseXI	GCAGC	7 cut(s)	249, 432, 454, 991, 1189, 1273, 1354
BsgI	GTGCAG	1 cut(s)	990
Bsh1236I	CGCG	1 cut(s)	177
BshFI	GGCC	6 cut(s)	33, 135, 838, 891, 1237, 1361
BshNI	GGYRCC	3 cut(s)	125, 633, 1066
BshTI	ACCGGT	1 cut(s)	1162
BsiHKAI	GWGCWC	1 cut(s)	643
BsiSI	CCGG	5 cut(s)	80, 644, 1109, 1163, 1329
BslFI	GGGAC	2 cut(s)	313, 1150
BslI	CCNNNNNNNGG	5 cut(s)	337, 643, 1049, 1076, 1233
BsmAI	GTCTC	3 cut(s)	165, 235, 384
BsmBI	CGTCTC	1 cut(s)	235
BsmFI	GGGAC	2 cut(s)	313, 1150
BsmI	GAATGC	2 cut(s)	1146, 1263
BsnI	GGCC	6 cut(s)	33, 135, 838, 891, 1237, 1361
Bsp1286I	GDGCHC	2 cut(s)	643, 1233
Bsp143I	GATC	6 cut(s)	100, 114, 480, 784, 982, 1223
Bsp1720I	GCTNAGC	2 cut(s)	663, 714
BspACI	CCGC	7 cut(s)	12, 21, 60, 175, 177, 265, 557
BspANI	GGCC	6 cut(s)	33, 135, 838, 891, 1237, 1361
BspCNI	CTCAG	2 cut(s)	706, 1309
BspFNI	CGCG	1 cut(s)	177
BspLI	GGNNCC	5 cut(s)	89, 127, 588, 635, 1068
BspMI	ACCTGC	2 cut(s)	966, 1257
BspPI	GGATC	3 cut(s)	488, 977, 1218
BspT107I	GGYRCC	3 cut(s)	125, 633, 1066
BsrDI	GCAATG	3 cut(s)	431, 1063, 1323
BsrFI	RCCGGY	2 cut(s)	1162, 1328
BsrI	ACTGG	2 cut(s)	482, 1177
BssAI	RCCGGY	2 cut(s)	1162, 1328
BssECI	CCNNGG	6 cut(s)	55, 175, 213, 655, 1075, 1232
BssMI	GATC	6 cut(s)	100, 114, 480, 784, 982, 1223
BssNI	GRCGYC	1 cut(s)	228
BssSI	CACGAG	2 cut(s)	278, 296
BssT1I	CCWWGG	3 cut(s)	213, 655, 1075
Bst2BI	CACGAG	2 cut(s)	278, 296
Bst2UI	CCWGG	3 cut(s)	137, 1050, 1234
Bst4CI	ACNGT	3 cut(s)	96, 221, 638
BstACI	GRCGYC	1 cut(s)	228
BstAPI	GCANNNNNTGC	1 cut(s)	1258
BstC8I	GCNNGC	4 cut(s)	19, 840, 1330, 1334
BstDEI	CTNAG	4 cut(s)	663, 714, 966, 1296
BstDSI	CCRYGG	2 cut(s)	55, 175
BstEII	GGTNACC	1 cut(s)	622
BstENI	CCTNNNNNAGG	2 cut(s)	1047, 1074
BstF5I	GGATG	6 cut(s)	243, 707, 817, 1221, 1311, 1410
BstFNI	CGCG	1 cut(s)	177
BstHHI	GCGC	2 cut(s)	17, 749
BstKTI	GATC	6 cut(s)	103, 117, 483, 787, 985, 1226
BstMAI	GTCTC	3 cut(s)	165, 235, 384
BstMBI	GATC	6 cut(s)	100, 114, 480, 784, 982, 1223
BstNI	CCWGG	3 cut(s)	137, 1050, 1234
BstNSI	RCATGY	3 cut(s)	328, 1198, 1336
BstPI	GGTNACC	1 cut(s)	622
BstSCI	CCNGG	3 cut(s)	135, 1048, 1232
BstSLI	GKGCMC	2 cut(s)	643, 1233
BstUI	CGCG	1 cut(s)	177
BstV1I	GCAGC	7 cut(s)	249, 432, 454, 991, 1189, 1273, 1354
BstX2I	RGATCY	1 cut(s)	1223
BstXI	CCANNNNNNTGG	1 cut(s)	62
BstYI	RGATCY	1 cut(s)	1223
BsuI	GTATCC	1 cut(s)	1406
BsuRI	GGCC	6 cut(s)	33, 135, 838, 891, 1237, 1361
BtgI	CCRYGG	2 cut(s)	55, 175
BtgZI	GCGATG	1 cut(s)	172
BtsCI	GGATG	6 cut(s)	243, 707, 817, 1221, 1311, 1410
BtsI	GCAGTG	1 cut(s)	237
BtsIMutI	CAGTG	1 cut(s)	237
BveI	ACCTGC	2 cut(s)	966, 1257
Cac8I	GCNNGC	4 cut(s)	19, 840, 1330, 1334
CfoI	GCGC	2 cut(s)	17, 749
Cfr10I	RCCGGY	2 cut(s)	1162, 1328
Cfr13I	GGNCC	2 cut(s)	153, 1360
Cfr42I	CCGCGG	1 cut(s)	178
CseI	GACGC	2 cut(s)	217, 1062
Csp6I	GTAC	3 cut(s)	97, 634, 1067
CspAI	ACCGGT	1 cut(s)	1162
CspCI	CAANNNNNGTGG	2 cut(s)	1111, 1146
CviAII	CATG	8 cut(s)	256, 325, 359, 1022, 1195, 1208, 1273, 1333
CviQI	GTAC	3 cut(s)	97, 634, 1067
DdeI	CTNAG	4 cut(s)	663, 714, 966, 1296
DpnI	GATC	6 cut(s)	102, 116, 482, 786, 984, 1225
DpnII	GATC	6 cut(s)	100, 114, 480, 784, 982, 1223
DraI	TTTAAA	1 cut(s)	498
EaeI	YGGCCR	1 cut(s)	836
Eco130I	CCWWGG	3 cut(s)	213, 655, 1075
Eco47I	GGWCC	1 cut(s)	153
Eco91I	GGTNACC	1 cut(s)	622
EcoNI	CCTNNNNNAGG	2 cut(s)	1047, 1074
EcoO65I	GGTNACC	1 cut(s)	622
EcoRII	CCWGG	3 cut(s)	135, 1048, 1232
EcoT14I	CCWWGG	3 cut(s)	213, 655, 1075
EcoT22I	ATGCAT	1 cut(s)	707
ErhI	CCWWGG	3 cut(s)	213, 655, 1075
Esp3I	CGTCTC	1 cut(s)	235
FaeI	CATG	8 cut(s)	259, 328, 362, 1025, 1198, 1211, 1276, 1336
FaqI	GGGAC	2 cut(s)	313, 1150
FatI	CATG	8 cut(s)	255, 324, 358, 1021, 1194, 1207, 1272, 1332
FauI	CCCGC	1 cut(s)	258
FauNDI	CATATG	1 cut(s)	952
FbaI	TGATCA	1 cut(s)	784
FblI	GTMKAC	1 cut(s)	1113
Fnu4HI	GCNGC	9 cut(s)	13, 22, 263, 443, 446, 1005, 1203, 1262, 1343
FokI	GGATG	6 cut(s)	230, 714, 804, 1228, 1318, 1417
Fsp4HI	GCNGC	9 cut(s)	13, 22, 263, 443, 446, 1005, 1203, 1262, 1343
FspBI	CTAG	2 cut(s)	827, 1143
GlaI	GCGC	2 cut(s)	16, 748
GluI	GCNGC	9 cut(s)	13, 22, 263, 443, 446, 1005, 1203, 1262, 1343
HaeIII	GGCC	6 cut(s)	33, 135, 838, 891, 1237, 1361
HapII	CCGG	5 cut(s)	80, 644, 1109, 1163, 1329
HgaI	GACGC	2 cut(s)	217, 1062
HhaI	GCGC	2 cut(s)	17, 749
Hin1I	GRCGYC	1 cut(s)	228
Hin1II	CATG	8 cut(s)	259, 328, 362, 1025, 1198, 1211, 1276, 1336
Hin6I	GCGC	2 cut(s)	15, 747
HinP1I	GCGC	2 cut(s)	15, 747
HincII	GTYRAC	2 cut(s)	7, 928
HindII	GTYRAC	2 cut(s)	7, 928
HinfI	GANTC	2 cut(s)	456, 1321
HpaI	GTTAAC	1 cut(s)	928
HpaII	CCGG	5 cut(s)	80, 644, 1109, 1163, 1329
HphI	GGTGA	3 cut(s)	233, 301, 1358
Hpy166II	GTNNAC	5 cut(s)	7, 301, 641, 928, 1114
Hpy188I	TCNGA	4 cut(s)	105, 607, 701, 1445
Hpy188III	TCNNGA	5 cut(s)	196, 298, 491, 1188, 1434
Hpy8I	GTNNAC	5 cut(s)	7, 301, 641, 928, 1114
HpyAV	CCTTC	5 cut(s)	116, 863, 1127, 1164, 1377
HpyCH4III	ACNGT	3 cut(s)	96, 221, 638
HpyCH4IV	ACGT	2 cut(s)	303, 1438
HpyF3I	CTNAG	4 cut(s)	663, 714, 966, 1296
HpySE526I	ACGT	2 cut(s)	303, 1438
Hsp92I	GRCGYC	1 cut(s)	228
Hsp92II	CATG	8 cut(s)	259, 328, 362, 1025, 1198, 1211, 1276, 1336
HspAI	GCGC	2 cut(s)	15, 747
KpnI	GGTACC	2 cut(s)	637, 1070
KroI	GCCGGC	1 cut(s)	1328
KroNI	GCCGGC	1 cut(s)	1330
Ksp22I	TGATCA	1 cut(s)	784
KspAI	GTTAAC	1 cut(s)	928
KspI	CCGCGG	1 cut(s)	178
Kzo9I	GATC	6 cut(s)	100, 114, 480, 784, 982, 1223
LmnI	GCTCC	4 cut(s)	93, 535, 592, 657
Lsp1109I	GCAGC	7 cut(s)	249, 432, 454, 991, 1189, 1273, 1354
LweI	GCATC	5 cut(s)	692, 714, 960, 1230, 1301
MaeI	CTAG	2 cut(s)	827, 1143
MaeII	ACGT	2 cut(s)	303, 1438
MaeIII	GTNAC	3 cut(s)	71, 473, 622
MalI	GATC	6 cut(s)	102, 116, 482, 786, 984, 1225
MboI	GATC	6 cut(s)	100, 114, 480, 784, 982, 1223
MboII	GAAGA	1 cut(s)	124
MflI	RGATCY	1 cut(s)	1223
MhlI	GDGCHC	2 cut(s)	643, 1233
MlsI	TGGCCA	1 cut(s)	838
MluCI	AATT	6 cut(s)	200, 412, 460, 552, 647, 1093
MluNI	TGGCCA	1 cut(s)	838
MlyI	GAGTC	1 cut(s)	450
MmeI	TCCRAC	3 cut(s)	115, 195, 679
MnlI	CCTC	9 cut(s)	23, 174, 341, 526, 941, 996, 1053, 1205, 1428
Mox20I	TGGCCA	1 cut(s)	838
Mph1103I	ATGCAT	1 cut(s)	707
MroNI	GCCGGC	1 cut(s)	1328
MscI	TGGCCA	1 cut(s)	838
MseI	TTAA	6 cut(s)	399, 426, 497, 593, 927, 1245
MslI	CAYNNNNRTG	5 cut(s)	60, 282, 323, 897, 1337
Msp20I	TGGCCA	1 cut(s)	838
MspA1I	CMGCKG	4 cut(s)	177, 445, 1004, 1202
MspI	CCGG	5 cut(s)	80, 644, 1109, 1163, 1329
MspR9I	CCNGG	3 cut(s)	137, 1050, 1234
Mva1269I	GAATGC	2 cut(s)	1146, 1263
MvaI	CCWGG	3 cut(s)	137, 1050, 1234
MvnI	CGCG	1 cut(s)	177
NaeI	GCCGGC	1 cut(s)	1330
NdeI	CATATG	1 cut(s)	952
NdeII	GATC	6 cut(s)	100, 114, 480, 784, 982, 1223
NgoMIV	GCCGGC	1 cut(s)	1328
NlaIII	CATG	8 cut(s)	259, 328, 362, 1025, 1198, 1211, 1276, 1336
NlaIV	GGNNCC	5 cut(s)	89, 127, 588, 635, 1068
NsiI	ATGCAT	1 cut(s)	707
NspI	RCATGY	3 cut(s)	328, 1198, 1336
OliI	CACNNNNGTG	1 cut(s)	60
PaeI	GCATGC	1 cut(s)	1336
PciI	ACATGT	2 cut(s)	324, 1194
PcsI	WCGNNNNNNNCGW	1 cut(s)	225
PctI	GAATGC	2 cut(s)	1146, 1263
PdiI	GCCGGC	1 cut(s)	1330
PfeI	GAWTC	1 cut(s)	1321
PflFI	GACNNNGTC	1 cut(s)	157
PinAI	ACCGGT	1 cut(s)	1162
PkrI	GCNGC	9 cut(s)	14, 23, 264, 444, 447, 1006, 1204, 1263, 1344
PleI	GAGTC	1 cut(s)	450
PpsI	GAGTC	1 cut(s)	450
PscI	ACATGT	2 cut(s)	324, 1194
PsiI	TTATAA	1 cut(s)	1284
Psp1406I	AACGTT	1 cut(s)	303
Psp6I	CCWGG	3 cut(s)	135, 1048, 1232
PspEI	GGTNACC	1 cut(s)	622
PspGI	CCWGG	3 cut(s)	135, 1048, 1232
PspN4I	GGNNCC	5 cut(s)	89, 127, 588, 635, 1068
PspPI	GGNCC	2 cut(s)	153, 1360
PsuI	RGATCY	1 cut(s)	1223
PsyI	GACNNNGTC	1 cut(s)	157
PvuII	CAGCTG	3 cut(s)	445, 1004, 1202
RsaI	GTAC	3 cut(s)	98, 635, 1068
RsaNI	GTAC	3 cut(s)	97, 634, 1067
RseI	CAYNNNNRTG	5 cut(s)	60, 282, 323, 897, 1337
SacII	CCGCGG	1 cut(s)	178
SaqAI	TTAA	6 cut(s)	399, 426, 497, 593, 927, 1245
SatI	GCNGC	9 cut(s)	13, 22, 263, 443, 446, 1005, 1203, 1262, 1343
Sau3AI	GATC	6 cut(s)	100, 114, 480, 784, 982, 1223
Sau96I	GGNCC	2 cut(s)	153, 1360
SchI	GAGTC	1 cut(s)	450
ScrFI	CCNGG	3 cut(s)	137, 1050, 1234
SduI	GDGCHC	2 cut(s)	643, 1233
SfaNI	GCATC	5 cut(s)	692, 714, 960, 1230, 1301
Sfr303I	CCGCGG	1 cut(s)	178
SgrBI	CCGCGG	1 cut(s)	178
SinI	GGWCC	1 cut(s)	153
SmiMI	CAYNNNNRTG	5 cut(s)	60, 282, 323, 897, 1337
SphI	GCATGC	1 cut(s)	1336
Sse9I	AATT	6 cut(s)	200, 412, 460, 552, 647, 1093
SsiI	CCGC	7 cut(s)	12, 21, 60, 175, 177, 265, 557
SspMI	CTAG	2 cut(s)	827, 1143
StyD4I	CCNGG	3 cut(s)	135, 1048, 1232
StyI	CCWWGG	3 cut(s)	213, 655, 1075
TaaI	ACNGT	3 cut(s)	96, 221, 638
TaiI	ACGT	2 cut(s)	306, 1441
TaqI	TCGA	2 cut(s)	345, 988
TasI	AATT	6 cut(s)	200, 412, 460, 552, 647, 1093
TauI	GCSGC	2 cut(s)	15, 24
TfiI	GAWTC	1 cut(s)	1321
Tru1I	TTAA	6 cut(s)	399, 426, 497, 593, 927, 1245
Tru9I	TTAA	6 cut(s)	399, 426, 497, 593, 927, 1245
TscAI	CASTG	1 cut(s)	244
TseI	GCWGC	7 cut(s)	262, 442, 445, 1004, 1202, 1261, 1342
TspDTI	ATGAA	5 cut(s)	296, 384, 476, 1232, 1334
TspGWI	ACGGA	3 cut(s)	156, 236, 1216
TspRI	CASTG	1 cut(s)	244
Tth111I	GACNNNGTC	1 cut(s)	157
VneI	GTGCAC	1 cut(s)	639
VpaK11BI	GGWCC	1 cut(s)	153
XagI	CCTNNNNNAGG	2 cut(s)	1047, 1074
XapI	RAATTY	1 cut(s)	200
XceI	RCATGY	3 cut(s)	328, 1198, 1336
XmiI	GTMKAC	1 cut(s)	1113
XspI	CTAG	2 cut(s)	827, 1143
Zsp2I	ATGCAT	1 cut(s)	707

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.