Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0005816

Physical Location & Seq

Genomic Coordinates Forward (+)

10110716 .. 10119010

Gene Structure 8295 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1137900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1476 bp

ATGGGTGACGGCAGCGCAAGCGGCAACAGAGACCATAACAACAATCACAACCACCACAACAACCACAGCCATGGCCACGGCCACAACCACAACCACAGCGGTGGTTATAGTAACAAGCCGGAGTGGCTGCAACAGTACGATCTCATAGGGAAGATCGGGGAGGGCACGTACGGCCTGGTGTTCCTTGTGAGGACCAAGTCTCCTTCCAACCGCGGAAAATCCATCGCCATCAAGAAATTCAAGCAGTCCAAGGACGGCGATGGCGTCTCCCCCACCGCCATCCGCGAAATCATGTTGCTGCGGGAAATATCGCACGAGAATGTGGTGAAGCTTGTGAATGTTCACATCAATCACACAGACATGTCCCTCTATCTGGCTTTCGATTACGCAGAACATGACCTTTATGAAATCATTAGACATCACAGAGACAAAGTTAATCAACCTATCAATCATTACACTATTAAGACATTGCTCTGGCAGCTGCTCAATGGACTCAATTATCTCCATAGTAATTGGATCATTCATCGCGATTTAAAGCCATCTAATATCTTGGTTATGGGCGATGGAGAGGAACAAGGAGTTGTGAAAATTGCGGATTTTGGACTTGCAAGAATATATCAGGCTCCTTTGAAGTCGTTGTCTGACAATGGGGTTGTGGTAACCATTTGGTACCGTGCGCCGGAGTTGCTCCTTGGGGCTAAGCACTACACAAGTGCTGTTGATATGTGGGCTGTTGGATGCATCTTTGCCGAGCTTTTGACTTTGAAGCCATTATTTCAAGGCGCAGAAGTCAAAGCTATAGCAAATCCTTTTCAGCTTGATCAACTTGACAAGATATTCAAGGTCTTAGGTCATCCGACACTAGAAAAGTGGCCAGCACTTGCAAACCTTCCACACTGGCAACAAGATATGCAACATATTCAAGGCCATAAATATGATAATACTGCATTGCATAGTGTAGTTCACCTCTCTCCAAAAAGTCCTGCATATGACCTCCTATCTAAGATGCTCGAATATGATCCTCGAAAGCGTATAACAGCTGCACAAGCATTAGAGCATGAGTATTTTCGTATCGAGCCTCTACCAGGTCGCAATGCTTTGGTGCCTACCCAAGGAGAGAAAGTAGTGAATTATCCTACTCGTCCGGTAGACACAACCACAGATTTTGAAGGGACAACTAGCATTCAACCTTCACAACCGGTATCGTCTGGAAATGCAGTATCTGGAAACATGTCAGGTGCTCATGTGGGGAGATCTGTACCCAGGCCTATGGTTAACATGCAAAGAATGCAGCCTCAGGGCATGACTGCTTATAATCTTGCATCTCAAGCAGGGATGGGTGGTGCAATGAATCCTGGCGGCCTGCCAATCCCGCGTGGTGTAGCACAGGCCCATCACCAACAACAGTTGAGAAGGAAAGATAATGGAATGGGGATGCCGGGATACCCTCCACAGCAGAAATCCAGACGTTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004693 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007346 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016592 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0031974 GO:0031981 GO:0032991 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0050789 GO:0050794 GO:0051726 GO:0065007 GO:0070013 GO:0071704 GO:0097472 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

492

Amino Acids

54.91

Weight (kDa)

9.22

Isoelectric Point (pI)

40.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	46 - 356	1.4e-66	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	49 - 255	3.3e-33	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005816)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G63610
fragaria_vesca	FvH4_1g25340 FvH4_1g25340 FvH4_1g25350
malus_domestica	MD00G1110700.v1.1 MD15G1137900.v1.1
prunus_persica	Prupe.4G280700_v2.0.a1
pyrus_communis	pycom1146g00040
rosa_chinensis	RchiOBHm_Chr2g0123381 RchiOBHm_Chr2g0123391
rosa_laevigata	RLG00000018746
rosa_multiflora	Rmu_sc0004330.1_g000003 Rmu_sc0024478.1_g000001 Rmu_sc0041165.1_g000001 Rmu_ssc0000345.1_g000001
rosa_roxburghii	Rroxscaffold_2G00120740 Rroxscaffold_2G00120750
rosa_rugosa	Rorug02G0244900 Rorug02G0244900 Rorug02G0245000
rosa_samantha	Rh2AG303700 Rh2BG311800 Rh2CG290300 Rh2CG290400 Rh2DG327800 Rh2DG327900
rosa_wichuraiana	Rw2G024310 Rw2G024320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1314
Acc65I	GGTACC	1 cut(s)	669
AccB1I	GGYRCC	2 cut(s)	669, 1102
AccI	GTMKAC	1 cut(s)	1149
AccII	CGCG	4 cut(s)	213, 285, 528, 1375
AclWI	GGATC	2 cut(s)	524, 1013
AcoI	YGGCCR	3 cut(s)	73, 79, 872
AcsI	RAATTY	1 cut(s)	236
AcyI	GRCGYC	1 cut(s)	264
AfaI	GTAC	4 cut(s)	137, 170, 671, 1260
AfiI	CCNNNNNNNGG	4 cut(s)	373, 679, 1085, 1112
AflIII	ACRYGT	2 cut(s)	360, 1230
AgeI	ACCGGT	1 cut(s)	1198
AgsI	TTSAA	8 cut(s)	241, 631, 766, 779, 841, 923, 1169, 1187
AjnI	CCWGG	4 cut(s)	174, 1084, 1262, 1354
AleI	CACNNNNGTG	1 cut(s)	99
AluBI	AGCT	6 cut(s)	331, 481, 754, 797, 817, 1040
AluI	AGCT	6 cut(s)	331, 481, 754, 797, 817, 1040
Alw21I	GWGCWC	1 cut(s)	1243
Alw26I	GTCTC	4 cut(s)	24, 204, 271, 420
AlwI	GGATC	2 cut(s)	524, 1013
AlwNI	CAGNNNCTG	1 cut(s)	1223
AoxI	GGCC	8 cut(s)	73, 79, 172, 872, 925, 1265, 1360, 1389
ApeKI	GCWGC	7 cut(s)	12, 127, 298, 478, 481, 1040, 1291
ApoI	RAATTY	1 cut(s)	236
AsiGI	ACCGGT	1 cut(s)	1198
Asp718I	GGTACC	1 cut(s)	669
AspLEI	GCGC	3 cut(s)	17, 679, 785
AspS9I	GGNCC	2 cut(s)	192, 1390
AsuC2I	CCSGG	1 cut(s)	1440
AsuHPI	GGTGA	4 cut(s)	17, 337, 956, 1388
AvaII	GGWCC	1 cut(s)	192
AxyI	CCTNAGG	1 cut(s)	1296
BaeGI	GKGCMC	1 cut(s)	167
BalI	TGGCCA	2 cut(s)	75, 874
BanI	GGYRCC	2 cut(s)	669, 1102
BauI	CACGAG	1 cut(s)	314
Bbv12I	GWGCWC	1 cut(s)	1243
BbvI	GCAGC	7 cut(s)	24, 114, 285, 468, 490, 1027, 1303
BccI	CCATC	8 cut(s)	230, 236, 254, 287, 547, 557, 1330, 1401
BceAI	ACGGC	4 cut(s)	25, 94, 187, 271
BciT130I	CCWGG	4 cut(s)	176, 1086, 1264, 1356
BciVI	GTATCC	1 cut(s)	1436
BclI	TGATCA	1 cut(s)	820
BcnI	CCSGG	1 cut(s)	1440
BcoDI	GTCTC	4 cut(s)	24, 204, 271, 420
BfaI	CTAG	2 cut(s)	863, 1179
BfmI	CTRYAG	1 cut(s)	798
BfuI	GTATCC	1 cut(s)	1436
BglI	GCCNNNNNGGC	1 cut(s)	124
BglII	AGATCT	1 cut(s)	1253
BisI	GCNGC	9 cut(s)	13, 22, 128, 299, 479, 482, 1041, 1292, 1360
BlpI	GCTNAGC	1 cut(s)	699
BlsI	GCNGC	9 cut(s)	14, 23, 129, 300, 480, 483, 1042, 1293, 1361
Bme1390I	CCNGG	5 cut(s)	176, 1086, 1264, 1356, 1440
Bme18I	GGWCC	1 cut(s)	192
BmgT120I	GGNCC	2 cut(s)	192, 1390
BmiI	GGNNCC	3 cut(s)	624, 671, 1104
BmrFI	CCNGG	5 cut(s)	176, 1086, 1264, 1356, 1440
BmsI	GCATC	5 cut(s)	728, 750, 996, 1331, 1425
Bpu1102I	GCTNAGC	1 cut(s)	699
BpuEI	CTTGAG	1 cut(s)	1311
BpuMI	CCSGG	1 cut(s)	1440
BsaAI	YACGTR	1 cut(s)	168
BsaHI	GRCGYC	1 cut(s)	264
BsaI	GGTCTC	1 cut(s)	24
BsaJI	CCNNGG	7 cut(s)	70, 76, 211, 249, 691, 1111, 1262
BsaWI	WCCGGW	2 cut(s)	1144, 1198
BsaXI	ACNNNNNCTCC	6 cut(s)	152, 182, 184, 214, 1107, 1137
Bsc4I	CCNNNNNNNGG	4 cut(s)	373, 679, 1085, 1112
Bse118I	RCCGGY	1 cut(s)	1198
Bse1I	ACTGG	1 cut(s)	902
Bse21I	CCTNAGG	1 cut(s)	1296
Bse3DI	GCAATG	4 cut(s)	467, 947, 1099, 1353
BseBI	CCWGG	4 cut(s)	176, 1086, 1264, 1356
BseDI	CCNNGG	7 cut(s)	70, 76, 211, 249, 691, 1111, 1262
BseGI	GGATG	5 cut(s)	279, 743, 853, 1341, 1440
BseLI	CCNNNNNNNGG	4 cut(s)	373, 679, 1085, 1112
BseMI	GCAATG	4 cut(s)	467, 947, 1099, 1353
BseMII	CTCAG	1 cut(s)	1310
BseNI	ACTGG	1 cut(s)	902
BseSI	GKGCMC	1 cut(s)	167
BseXI	GCAGC	7 cut(s)	24, 114, 285, 468, 490, 1027, 1303
BsgI	GTGCAG	1 cut(s)	1026
Bsh1236I	CGCG	4 cut(s)	213, 285, 528, 1375
BshFI	GGCC	8 cut(s)	75, 81, 174, 874, 927, 1267, 1362, 1391
BshNI	GGYRCC	2 cut(s)	669, 1102
BshTI	ACCGGT	1 cut(s)	1198
BsiHKAI	GWGCWC	1 cut(s)	1243
BsiSI	CCGG	5 cut(s)	119, 680, 1145, 1199, 1439
BsiWI	CGTACG	1 cut(s)	168
BslFI	GGGAC	2 cut(s)	349, 1186
BslI	CCNNNNNNNGG	4 cut(s)	373, 679, 1085, 1112
BsmAI	GTCTC	4 cut(s)	24, 204, 271, 420
BsmBI	CGTCTC	1 cut(s)	271
BsmFI	GGGAC	2 cut(s)	349, 1186
BsmI	GAATGC	2 cut(s)	1182, 1293
BsnI	GGCC	8 cut(s)	75, 81, 174, 874, 927, 1267, 1362, 1391
Bso31I	GGTCTC	1 cut(s)	24
Bsp1286I	GDGCHC	2 cut(s)	167, 1243
Bsp143I	GATC	6 cut(s)	139, 153, 516, 820, 1018, 1253
Bsp1720I	GCTNAGC	1 cut(s)	699
Bsp19I	CCATGG	1 cut(s)	70
Bsp68I	TCGCGA	1 cut(s)	528
BspANI	GGCC	8 cut(s)	75, 81, 174, 874, 927, 1267, 1362, 1391
BspCNI	CTCAG	1 cut(s)	1309
BspFNI	CGCG	4 cut(s)	213, 285, 528, 1375
BspLI	GGNNCC	3 cut(s)	624, 671, 1104
BspPI	GGATC	2 cut(s)	524, 1013
BspT107I	GGYRCC	2 cut(s)	669, 1102
BspTNI	GGTCTC	1 cut(s)	24
BsrDI	GCAATG	4 cut(s)	467, 947, 1099, 1353
BsrFI	RCCGGY	1 cut(s)	1198
BsrI	ACTGG	1 cut(s)	902
BssAI	RCCGGY	1 cut(s)	1198
BssECI	CCNNGG	7 cut(s)	70, 76, 211, 249, 691, 1111, 1262
BssMI	GATC	6 cut(s)	139, 153, 516, 820, 1018, 1253
BssNI	GRCGYC	1 cut(s)	264
BssSI	CACGAG	1 cut(s)	314
BssT1I	CCWWGG	4 cut(s)	70, 249, 691, 1111
Bst2BI	CACGAG	1 cut(s)	314
Bst2UI	CCWGG	4 cut(s)	176, 1086, 1264, 1356
Bst4CI	ACNGT	3 cut(s)	135, 674, 1407
BstACI	GRCGYC	1 cut(s)	264
BstAPI	GCANNNNNTGC	1 cut(s)	1288
BstBAI	YACGTR	1 cut(s)	168
BstC8I	GCNNGC	3 cut(s)	19, 876, 1364
BstDEI	CTNAG	4 cut(s)	699, 847, 1002, 1296
BstDSI	CCRYGG	3 cut(s)	70, 76, 211
BstEII	GGTNACC	1 cut(s)	658
BstENI	CCTNNNNNAGG	2 cut(s)	1083, 1110
BstF5I	GGATG	5 cut(s)	279, 743, 853, 1341, 1440
BstFNI	CGCG	4 cut(s)	213, 285, 528, 1375
BstHHI	GCGC	3 cut(s)	17, 679, 785
BstKTI	GATC	6 cut(s)	142, 156, 519, 823, 1021, 1256
BstMAI	GTCTC	4 cut(s)	24, 204, 271, 420
BstMBI	GATC	6 cut(s)	139, 153, 516, 820, 1018, 1253
BstNI	CCWGG	4 cut(s)	176, 1086, 1264, 1356
BstNSI	RCATGY	3 cut(s)	364, 1234, 1282
BstPI	GGTNACC	1 cut(s)	658
BstSCI	CCNGG	5 cut(s)	174, 1084, 1262, 1354, 1438
BstSFI	CTRYAG	1 cut(s)	798
BstSLI	GKGCMC	1 cut(s)	167
BstUI	CGCG	4 cut(s)	213, 285, 528, 1375
BstV1I	GCAGC	7 cut(s)	24, 114, 285, 468, 490, 1027, 1303
BstX2I	RGATCY	1 cut(s)	1253
BstXI	CCANNNNNNTGG	3 cut(s)	71, 101, 1270
BstYI	RGATCY	1 cut(s)	1253
Bsu36I	CCTNAGG	1 cut(s)	1296
BsuI	GTATCC	1 cut(s)	1436
BsuRI	GGCC	8 cut(s)	75, 81, 174, 874, 927, 1267, 1362, 1391
BtgI	CCRYGG	3 cut(s)	70, 76, 211
BtgZI	GCGATG	4 cut(s)	208, 273, 509, 576
BtsCI	GGATG	5 cut(s)	279, 743, 853, 1341, 1440
BtsIMutI	CAGTG	1 cut(s)	895
BtuMI	TCGCGA	1 cut(s)	528
Cac8I	GCNNGC	3 cut(s)	19, 876, 1364
CaiI	CAGNNNCTG	1 cut(s)	1223
CfoI	GCGC	3 cut(s)	17, 679, 785
Cfr10I	RCCGGY	1 cut(s)	1198
Cfr13I	GGNCC	2 cut(s)	192, 1390
Cfr42I	CCGCGG	1 cut(s)	214
CseI	GACGC	1 cut(s)	253
CsiI	ACCWGGT	1 cut(s)	1084
Csp6I	GTAC	4 cut(s)	136, 169, 670, 1259
CspAI	ACCGGT	1 cut(s)	1198
CspCI	CAANNNNNGTGG	2 cut(s)	1147, 1182
CviAII	CATG	9 cut(s)	71, 292, 361, 395, 1058, 1231, 1244, 1279, 1303
CviQI	GTAC	4 cut(s)	136, 169, 670, 1259
DdeI	CTNAG	4 cut(s)	699, 847, 1002, 1296
DpnI	GATC	6 cut(s)	141, 155, 518, 822, 1020, 1255
DpnII	GATC	6 cut(s)	139, 153, 516, 820, 1018, 1253
DraI	TTTAAA	1 cut(s)	534
EaeI	YGGCCR	3 cut(s)	73, 79, 872
Eco130I	CCWWGG	4 cut(s)	70, 249, 691, 1111
Eco147I	AGGCCT	1 cut(s)	1267
Eco31I	GGTCTC	1 cut(s)	24
Eco47I	GGWCC	1 cut(s)	192
Eco81I	CCTNAGG	1 cut(s)	1296
Eco91I	GGTNACC	1 cut(s)	658
EcoNI	CCTNNNNNAGG	2 cut(s)	1083, 1110
EcoO65I	GGTNACC	1 cut(s)	658
EcoRII	CCWGG	4 cut(s)	174, 1084, 1262, 1354
EcoT14I	CCWWGG	4 cut(s)	70, 249, 691, 1111
EcoT22I	ATGCAT	1 cut(s)	743
ErhI	CCWWGG	4 cut(s)	70, 249, 691, 1111
Esp3I	CGTCTC	1 cut(s)	271
FaeI	CATG	9 cut(s)	74, 295, 364, 398, 1061, 1234, 1247, 1282, 1306
FaqI	GGGAC	2 cut(s)	349, 1186
FatI	CATG	9 cut(s)	70, 291, 360, 394, 1057, 1230, 1243, 1278, 1302
FauI	CCCGC	2 cut(s)	294, 1380
FauNDI	CATATG	1 cut(s)	988
FbaI	TGATCA	1 cut(s)	820
FblI	GTMKAC	1 cut(s)	1149
Fnu4HI	GCNGC	9 cut(s)	13, 22, 128, 299, 479, 482, 1041, 1292, 1360
FokI	GGATG	5 cut(s)	266, 750, 840, 1348, 1447
Fsp4HI	GCNGC	9 cut(s)	13, 22, 128, 299, 479, 482, 1041, 1292, 1360
FspBI	CTAG	2 cut(s)	863, 1179
GlaI	GCGC	3 cut(s)	16, 678, 784
GluI	GCNGC	9 cut(s)	13, 22, 128, 299, 479, 482, 1041, 1292, 1360
HaeIII	GGCC	8 cut(s)	75, 81, 174, 874, 927, 1267, 1362, 1391
HapII	CCGG	5 cut(s)	119, 680, 1145, 1199, 1439
HgaI	GACGC	1 cut(s)	253
HhaI	GCGC	3 cut(s)	17, 679, 785
Hin1I	GRCGYC	1 cut(s)	264
Hin1II	CATG	9 cut(s)	74, 295, 364, 398, 1061, 1234, 1247, 1282, 1306
Hin6I	GCGC	3 cut(s)	15, 677, 783
HinP1I	GCGC	3 cut(s)	15, 677, 783
HincII	GTYRAC	1 cut(s)	1276
HindII	GTYRAC	1 cut(s)	1276
HindIII	AAGCTT	1 cut(s)	329
HinfI	GANTC	2 cut(s)	492, 1351
HpaI	GTTAAC	1 cut(s)	1276
HpaII	CCGG	5 cut(s)	119, 680, 1145, 1199, 1439
HphI	GGTGA	4 cut(s)	17, 337, 956, 1388
Hpy166II	GTNNAC	4 cut(s)	343, 964, 1150, 1276
Hpy188I	TCNGA	3 cut(s)	643, 858, 1475
Hpy188III	TCNNGA	5 cut(s)	232, 527, 1209, 1224, 1464
Hpy8I	GTNNAC	4 cut(s)	343, 964, 1150, 1276
HpyAV	CCTTC	5 cut(s)	213, 899, 1163, 1200, 1407
HpyCH4III	ACNGT	3 cut(s)	135, 674, 1407
HpyCH4IV	ACGT	2 cut(s)	167, 1468
HpyF3I	CTNAG	4 cut(s)	699, 847, 1002, 1296
HpySE526I	ACGT	2 cut(s)	167, 1468
Hsp92I	GRCGYC	1 cut(s)	264
Hsp92II	CATG	9 cut(s)	74, 295, 364, 398, 1061, 1234, 1247, 1282, 1306
HspAI	GCGC	3 cut(s)	15, 677, 783
KpnI	GGTACC	1 cut(s)	673
Ksp22I	TGATCA	1 cut(s)	820
KspAI	GTTAAC	1 cut(s)	1276
KspI	CCGCGG	1 cut(s)	214
Kzo9I	GATC	6 cut(s)	139, 153, 516, 820, 1018, 1253
LmnI	GCTCC	2 cut(s)	628, 693
Lsp1109I	GCAGC	7 cut(s)	24, 114, 285, 468, 490, 1027, 1303
LweI	GCATC	5 cut(s)	728, 750, 996, 1331, 1425
MabI	ACCWGGT	1 cut(s)	1084
MaeI	CTAG	2 cut(s)	863, 1179
MaeII	ACGT	2 cut(s)	167, 1468
MaeIII	GTNAC	3 cut(s)	5, 110, 658
MalI	GATC	6 cut(s)	141, 155, 518, 822, 1020, 1255
MboI	GATC	6 cut(s)	139, 153, 516, 820, 1018, 1253
MboII	GAAGA	1 cut(s)	163
MflI	RGATCY	1 cut(s)	1253
MhlI	GDGCHC	2 cut(s)	167, 1243
MlsI	TGGCCA	2 cut(s)	75, 874
MluCI	AATT	5 cut(s)	236, 496, 511, 588, 1129
MluNI	TGGCCA	2 cut(s)	75, 874
MlyI	GAGTC	1 cut(s)	486
MmeI	TCCRAC	3 cut(s)	231, 715, 881
Mox20I	TGGCCA	2 cut(s)	75, 874
Mph1103I	ATGCAT	1 cut(s)	743
MscI	TGGCCA	2 cut(s)	75, 874
MseI	TTAA	4 cut(s)	435, 462, 533, 1275
MslI	CAYNNNNRTG	5 cut(s)	69, 99, 318, 359, 933
Msp20I	TGGCCA	2 cut(s)	75, 874
MspA1I	CMGCKG	4 cut(s)	99, 213, 481, 1040
MspI	CCGG	5 cut(s)	119, 680, 1145, 1199, 1439
MspR9I	CCNGG	5 cut(s)	176, 1086, 1264, 1356, 1440
Mva1269I	GAATGC	2 cut(s)	1182, 1293
MvaI	CCWGG	4 cut(s)	176, 1086, 1264, 1356
MvnI	CGCG	4 cut(s)	213, 285, 528, 1375
NciI	CCSGG	1 cut(s)	1440
NcoI	CCATGG	1 cut(s)	70
NdeI	CATATG	1 cut(s)	988
NdeII	GATC	6 cut(s)	139, 153, 516, 820, 1018, 1253
NlaIII	CATG	9 cut(s)	74, 295, 364, 398, 1061, 1234, 1247, 1282, 1306
NlaIV	GGNNCC	3 cut(s)	624, 671, 1104
NmeAIII	GCCGAG	1 cut(s)	775
NmuCI	GTSAC	1 cut(s)	5
NruI	TCGCGA	1 cut(s)	528
NsiI	ATGCAT	1 cut(s)	743
NspI	RCATGY	3 cut(s)	364, 1234, 1282
OliI	CACNNNNGTG	1 cut(s)	99
PceI	AGGCCT	1 cut(s)	1267
PciI	ACATGT	2 cut(s)	360, 1230
PcsI	WCGNNNNNNNCGW	1 cut(s)	261
PctI	GAATGC	2 cut(s)	1182, 1293
PfeI	GAWTC	1 cut(s)	1351
Pfl23II	CGTACG	1 cut(s)	168
PflFI	GACNNNGTC	1 cut(s)	196
PinAI	ACCGGT	1 cut(s)	1198
PkrI	GCNGC	9 cut(s)	14, 23, 129, 300, 480, 483, 1042, 1293, 1361
PleI	GAGTC	1 cut(s)	486
PpsI	GAGTC	1 cut(s)	486
Ppu21I	YACGTR	1 cut(s)	168
PscI	ACATGT	2 cut(s)	360, 1230
PsiI	TTATAA	1 cut(s)	1314
Psp6I	CCWGG	4 cut(s)	174, 1084, 1262, 1354
PspEI	GGTNACC	1 cut(s)	658
PspGI	CCWGG	4 cut(s)	174, 1084, 1262, 1354
PspLI	CGTACG	1 cut(s)	168
PspN4I	GGNNCC	3 cut(s)	624, 671, 1104
PspPI	GGNCC	2 cut(s)	192, 1390
PstNI	CAGNNNCTG	1 cut(s)	1223
PsuI	RGATCY	1 cut(s)	1253
PsyI	GACNNNGTC	1 cut(s)	196
PvuII	CAGCTG	2 cut(s)	481, 1040
RruI	TCGCGA	1 cut(s)	528
RsaI	GTAC	4 cut(s)	137, 170, 671, 1260
RsaNI	GTAC	4 cut(s)	136, 169, 670, 1259
RseI	CAYNNNNRTG	5 cut(s)	69, 99, 318, 359, 933
SacII	CCGCGG	1 cut(s)	214
SaqAI	TTAA	4 cut(s)	435, 462, 533, 1275
SatI	GCNGC	9 cut(s)	13, 22, 128, 299, 479, 482, 1041, 1292, 1360
Sau3AI	GATC	6 cut(s)	139, 153, 516, 820, 1018, 1253
Sau96I	GGNCC	2 cut(s)	192, 1390
SchI	GAGTC	1 cut(s)	486
ScrFI	CCNGG	5 cut(s)	176, 1086, 1264, 1356, 1440
SduI	GDGCHC	2 cut(s)	167, 1243
SexAI	ACCWGGT	1 cut(s)	1084
SfaNI	GCATC	5 cut(s)	728, 750, 996, 1331, 1425
SfcI	CTRYAG	1 cut(s)	798
Sfr303I	CCGCGG	1 cut(s)	214
SgrBI	CCGCGG	1 cut(s)	214
SinI	GGWCC	1 cut(s)	192
SmiMI	CAYNNNNRTG	5 cut(s)	69, 99, 318, 359, 933
SmlI	CTYRAG	1 cut(s)	1326
SmoI	CTYRAG	1 cut(s)	1326
Sse9I	AATT	5 cut(s)	236, 496, 511, 588, 1129
SseBI	AGGCCT	1 cut(s)	1267
SspMI	CTAG	2 cut(s)	863, 1179
StuI	AGGCCT	1 cut(s)	1267
StyD4I	CCNGG	5 cut(s)	174, 1084, 1262, 1354, 1438
StyI	CCWWGG	4 cut(s)	70, 249, 691, 1111
TaaI	ACNGT	3 cut(s)	135, 674, 1407
TaiI	ACGT	2 cut(s)	170, 1471
TaqI	TCGA	4 cut(s)	381, 1011, 1024, 1074
TasI	AATT	5 cut(s)	236, 496, 511, 588, 1129
TauI	GCSGC	2 cut(s)	24, 1362
TfiI	GAWTC	1 cut(s)	1351
Tru1I	TTAA	4 cut(s)	435, 462, 533, 1275
Tru9I	TTAA	4 cut(s)	435, 462, 533, 1275
TscAI	CASTG	1 cut(s)	902
TseFI	GTSAC	1 cut(s)	5
TseI	GCWGC	7 cut(s)	12, 127, 298, 478, 481, 1040, 1291
Tsp45I	GTSAC	1 cut(s)	5
TspDTI	ATGAA	3 cut(s)	420, 512, 1364
TspRI	CASTG	1 cut(s)	902
Tth111I	GACNNNGTC	1 cut(s)	196
VpaK11BI	GGWCC	1 cut(s)	192
XagI	CCTNNNNNAGG	2 cut(s)	1083, 1110
XapI	RAATTY	1 cut(s)	236
XceI	RCATGY	3 cut(s)	364, 1234, 1282
XmiI	GTMKAC	1 cut(s)	1149
XspI	CTAG	2 cut(s)	863, 1179
Zsp2I	ATGCAT	1 cut(s)	743

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.