Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0005816

Physical Location & Seq

Genomic Coordinates Reverse (-)

53098400 .. 53101963

Gene Structure 3564 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00120740.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2292 bp

ATGCATATTGTATTCCAAAGCTTGCAATCTGGTAGTAGAGGATCAGCTTGCCGCATTACCAAAGCCGTTTTCTTCCTACCCGAATTTGTCACCCCTATTTCTATCCCAAGGGCTACTGTTTCACGTAGTGAAGTGTTCATAGTCCGAGTCCAAAACGACCTGAAACCGCCACAACACTCCATAAACCATGAATGGTACGCTTCCACCCTCAACACTCTGTCTGCAACAAACGACTCCCTCCATTACGTCTACAACACTGTCTTTCCTGGCTTCTCCGCCGACCTCACCAGACAACAAGCCAACCAGCTACGCCAGCGTCCAGAAATAGTAAGCGTTTTCCGTGACAGACTCCTCAAACATCATACAACTAGGTCTCCTGCATTCCTCGGCCTCAACACAAACGAAACCAACGACCAGGGAATTCTGAAACTGTCTGATTCCGGTGCCAACGTCATCATCGGTGTCATCGACAGCGGCATATGGCCAGAACACCGCAGCTTCCACGACCATGGCCTCGGACCAATCCCTTCACATTGGAAAGGCCGCTGCCTACCCGGAGATTCCGAAAATGCCGCCACCTTTTGCAACAGAAAGCTCATCGGAGCAAGATACTTCACCCGAGGCATCAACAACGAAACTGAAGTTTCTTCCGCTAGAGACGACGTTGGCCATGGAACACACACGGCGTCAACTATGGCTGGACGACACGTCGGAAGCGCATCTTTCCTCGGCTTCGCCAAAGGTGTTGCATCTGGAATTGCCCCAAAAGCACGACTTGCAGTCTACAGAGTGTGTGGGAAGATAGGGTGTGCGCAATCCGACGTACTCGCCGGTATCGAACAGGCCGTACAGGACGGCGTCGACGTCATCTCTATGTCCCTCGGCGGCGACTCCTCCTTGCAGTACGATAAGGATCCGATTGCAATCGCCACATTCGCCGCAACCGAGAAAGGCATTGTCTGTTCGGCCTCTGGAGGCAATGCGGGCCCAGATCACGGCACCGTGACTAACGTCGCACCGTGGATCACCACCGTCGGTGCAAGCACGATTGACCGTGACTTCGGCGCTGATTTAGTGCTTGGTAATGGTCGCGTCATCGCTGGTTGTTCTCTTTACCATGGCACTTGGCCGGAGAAGACATCCGCACCGTTGGTTTACACCGGTGGGATATGCATGGAGCTTACCAATAAGAAACGCATCCCCGACTTCGTCCGAGGAAACATCGTGGTTTGCAATTATATCAGCACGGTTTTCCCTGAAGATTTCGACAGGATCAGGAATGCTGGTGCGGTGGGAATGGTCGGGATTGAACAAAACGACCTATTGGCAACTCCATATTCTATTCCTGCACTGGCGATCAGACGATCAGCCCGTCACGAACTTCTCCGGTATATGAACGTCACCAAGAATCCACGTGCAGCAATGCGCTTCCTTGGGACCGTTACAGGGGTGAAGCCTGCACCGGTGGTAGCCGACTTTTCGTCGCGAGGACCCAATCTGTTGTCTCCGTTTGTTGTGAAGCCCGATGTCATAGCGCCGGGTGTCAACATCTTAGCGGCTTGGCCGCAATTCGATGATTTGCCCGATTTCCGTATAATGTCAGGGACGTCCATGTCTTGCCCCCACGTGTCGGGAATAGCTGCATTGTTGAGAGGGGCCCGCCCTAGCTGGTCTCCCGCCATGATCAAATCGGCCATGATGACCACGGCTTACACAAAGTATCGCGACGGAGGGCCGCTGCTAGATGAGTTGCATCGCCACCAGGTGGTGTGGGGGATCGGTGCCGGACACGTGGATCCTCAGAAAGCTCTTGATCCTGGTTTGGTGTATGATCTTAAGGCGGATGACTATATCAACTTTTTGTGTGCGTCGAATTATAGTGATGAACAAGTAAGGTCAGTCACACAAAAGACTACTGCCTGCAAGGGCAGGAAAGCAGGCTTCACTCAACCGTGGGATTTCAACTACCCAGCTATATCGCTGGTTTTTGATGTGACGCGGTGGCCAAAATCGGAGGATGTTATTGTTCGGAAAAGTGTTACAAATGTTGGTGAAGGTTCCTCCACTTACATTGTACGTAGTGTCATCAATCCATTTGGGGTCAAGGTTCGTGTTATTCCCCCTAAATTGAAGTTCTCAAAGAAAGGCGAAATTCAGAGTTACGTTGTCAAGTTTACGGCTAATAAGAATGTGGGAGTGCCGCGACATGGGAATGTGGCGTTTGGCCAGCTGATTTGGACAGATGGGAAACATAAGGTTTCTTCACCGATTACGGTGACATATATGGGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8

Protein Analysis

763

Amino Acids

82.75

Weight (kDa)

9.25

Isoelectric Point (pI)

34.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	45 - 120	7e-14	Peptidase inhibitor I9
Peptidase_S8	PF00082	148 - 574	1.3e-47	Subtilase family
fn3_6	PF17766	653 - 759	9.7e-24	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005816)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G63610
fragaria_vesca	FvH4_1g25340 FvH4_1g25340 FvH4_1g25350
malus_domestica	MD00G1110700.v1.1 MD15G1137900.v1.1
prunus_persica	Prupe.4G280700_v2.0.a1
pyrus_communis	pycom1146g00040
rosa_chinensis	RchiOBHm_Chr2g0123381 RchiOBHm_Chr2g0123391
rosa_laevigata	RLG00000018746
rosa_multiflora	Rmu_sc0004330.1_g000003 Rmu_sc0024478.1_g000001 Rmu_sc0041165.1_g000001 Rmu_ssc0000345.1_g000001
rosa_roxburghii	Rroxscaffold_2G00120740 Rroxscaffold_2G00120750
rosa_rugosa	Rorug02G0244900 Rorug02G0244900 Rorug02G0245000
rosa_samantha	Rh2AG303700 Rh2BG311800 Rh2CG290300 Rh2CG290400 Rh2DG327800 Rh2DG327900
rosa_wichuraiana	Rw2G024310 Rw2G024320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1612
AatII	GACGTC	2 cut(s)	867, 1610
Acc16I	TGCGCA	1 cut(s)	813
AccB1I	GGYRCC	3 cut(s)	443, 998, 1781
AccB7I	CCANNNNNTGG	1 cut(s)	1765
AccI	GTMKAC	3 cut(s)	249, 783, 861
AccII	CGCG	5 cut(s)	1092, 1486, 1725, 1999, 2203
AclWI	GGATC	9 cut(s)	49, 908, 921, 1031, 1280, 1784, 1790, 1803, 1808
AcoI	YGGCCR	7 cut(s)	482, 667, 1127, 1562, 1692, 2003, 2224
AcsI	RAATTY	3 cut(s)	83, 420, 2151
AcuI	CTGAAG	2 cut(s)	660, 1278
AcvI	CACGTG	3 cut(s)	1415, 1627, 1792
AcyI	GRCGYC	4 cut(s)	686, 858, 864, 1607
AdeI	CACNNNGTG	2 cut(s)	128, 1765
AfaI	GTAC	5 cut(s)	197, 825, 849, 905, 2076
AfiI	CCNNNNNNNGG	5 cut(s)	995, 1446, 1630, 1765, 2207
AflII	CTTAAG	1 cut(s)	1835
AflIII	ACRYGT	3 cut(s)	706, 1626, 1789
AgeI	ACCGGT	2 cut(s)	1160, 1462
AgsI	TTSAA	3 cut(s)	1310, 1963, 2131
AhdI	GACNNNNNGTC	3 cut(s)	707, 779, 1480
AjiI	CACGTC	1 cut(s)	709
AjnI	CCWGG	4 cut(s)	265, 414, 1761, 1816
AjuI	GAANNNNNNNTTGG	2 cut(s)	53, 85
Alw26I	GTCTC	4 cut(s)	378, 651, 1509, 1677
AlwI	GGATC	9 cut(s)	49, 908, 921, 1031, 1280, 1784, 1790, 1803, 1808
Ama87I	CYCGRG	1 cut(s)	618
ApaI	GGGCCC	2 cut(s)	989, 1660
ApeKI	GCWGC	5 cut(s)	495, 546, 1418, 1640, 1738
ApoI	RAATTY	3 cut(s)	83, 420, 2151
ArsI	GACNNNNNNTTYG	2 cut(s)	1043, 1075
AsiGI	ACCGGT	2 cut(s)	1160, 1462
AspLEI	GCGC	5 cut(s)	719, 814, 1067, 1428, 1537
AspS9I	GGNCC	8 cut(s)	518, 985, 986, 1437, 1490, 1656, 1657, 1733
AsuC2I	CCSGG	2 cut(s)	555, 1539
AsuHPI	GGTGA	9 cut(s)	82, 277, 607, 1018, 1393, 1462, 2063, 2256, 2287
AvaI	CYCGRG	1 cut(s)	618
AvaII	GGWCC	3 cut(s)	518, 1437, 1490
BaeGI	GKGCMC	2 cut(s)	989, 1660
BalI	TGGCCA	4 cut(s)	484, 669, 2005, 2226
BamHI	GGATCC	2 cut(s)	913, 1795
BanI	GGYRCC	3 cut(s)	443, 998, 1781
BanII	GRGCYC	2 cut(s)	989, 1660
BbrPI	CACGTG	3 cut(s)	1415, 1627, 1792
BbsI	GAAGAC	1 cut(s)	1142
BbvI	GCAGC	5 cut(s)	507, 533, 1430, 1627, 1725
BccI	CCATC	1 cut(s)	2237
BceAI	ACGGC	7 cut(s)	50, 699, 830, 871, 1012, 1722, 2193
BciT130I	CCWGG	4 cut(s)	267, 416, 1763, 1818
BclI	TGATCA	1 cut(s)	1683
BcnI	CCSGG	2 cut(s)	555, 1539
BcoDI	GTCTC	4 cut(s)	378, 651, 1509, 1677
BfaI	CTAG	4 cut(s)	369, 654, 1665, 1742
BfmI	CTRYAG	1 cut(s)	784
BfoI	RGCGCY	2 cut(s)	1068, 1538
BfrI	CTTAAG	1 cut(s)	1835
Bme1390I	CCNGG	6 cut(s)	267, 416, 555, 1539, 1763, 1818
Bme18I	GGWCC	3 cut(s)	518, 1437, 1490
BmeRI	GACNNNNNGTC	3 cut(s)	707, 779, 1480
BmeT110I	CYCGRG	1 cut(s)	618
BmgBI	CACGTC	1 cut(s)	709
BmgT120I	GGNCC	8 cut(s)	518, 985, 986, 1437, 1490, 1656, 1657, 1733
BmrFI	CCNGG	6 cut(s)	267, 416, 555, 1539, 1763, 1818
BmsI	GCATC	5 cut(s)	633, 728, 758, 1206, 1762
BoxI	GACNNNNGTC	1 cut(s)	1010
BpiI	GAAGAC	1 cut(s)	1142
BpmI	CTGGAG	1 cut(s)	993
BpuMI	CCSGG	2 cut(s)	555, 1539
BsaAI	YACGTR	5 cut(s)	125, 1415, 1627, 1792, 2078
BsaBI	GATNNNNATC	2 cut(s)	912, 923
BsaHI	GRCGYC	4 cut(s)	686, 858, 864, 1607
BsaI	GGTCTC	2 cut(s)	378, 1677
BsaWI	WCCGGW	4 cut(s)	440, 1160, 1386, 1462
BsaXI	ACNNNNNCTCC	2 cut(s)	358, 388
Bsc4I	CCNNNNNNNGG	5 cut(s)	995, 1446, 1630, 1765, 2207
Bse118I	RCCGGY	3 cut(s)	830, 1160, 1462
Bse1I	ACTGG	1 cut(s)	1356
Bse3DI	GCAATG	2 cut(s)	985, 1428
Bse8I	GATNNNNATC	2 cut(s)	912, 923
BseBI	CCWGG	4 cut(s)	267, 416, 1763, 1818
BseGI	GGATG	4 cut(s)	1139, 1197, 1849, 2023
BseJI	GATNNNNATC	2 cut(s)	912, 923
BseLI	CCNNNNNNNGG	5 cut(s)	995, 1446, 1630, 1765, 2207
BseMI	GCAATG	2 cut(s)	985, 1428
BseMII	CTCAG	1 cut(s)	1814
BseNI	ACTGG	1 cut(s)	1356
BseRI	GAGGAG	2 cut(s)	341, 883
BseSI	GKGCMC	2 cut(s)	989, 1660
BseXI	GCAGC	5 cut(s)	507, 533, 1430, 1627, 1725
BseYI	CCCAGC	1 cut(s)	1969
BsgI	GTGCAG	3 cut(s)	1332, 1437, 1443
Bsh1236I	CGCG	5 cut(s)	1092, 1486, 1725, 1999, 2203
BshNI	GGYRCC	3 cut(s)	443, 998, 1781
BshTI	ACCGGT	2 cut(s)	1160, 1462
BsiHKCI	CYCGRG	1 cut(s)	618
BsiSI	CCGG	9 cut(s)	441, 555, 831, 1130, 1161, 1387, 1463, 1538, 1785
BslFI	GGGAC	3 cut(s)	862, 1450, 1618
BslI	CCNNNNNNNGG	5 cut(s)	995, 1446, 1630, 1765, 2207
BsmAI	GTCTC	4 cut(s)	378, 651, 1509, 1677
BsmBI	CGTCTC	1 cut(s)	651
BsmFI	GGGAC	3 cut(s)	862, 1450, 1618
BsmI	GAATGC	2 cut(s)	380, 1285
Bso31I	GGTCTC	2 cut(s)	378, 1677
BsoBI	CYCGRG	1 cut(s)	618
Bsp120I	GGGCCC	2 cut(s)	985, 1656
Bsp1286I	GDGCHC	2 cut(s)	989, 1660
Bsp19I	CCATGG	3 cut(s)	508, 670, 1117
Bsp68I	TCGCGA	2 cut(s)	1486, 1725
BspCNI	CTCAG	1 cut(s)	1813
BspFNI	CGCG	5 cut(s)	1092, 1486, 1725, 1999, 2203
BspPI	GGATC	9 cut(s)	49, 908, 921, 1031, 1280, 1784, 1790, 1803, 1808
BspT107I	GGYRCC	3 cut(s)	443, 998, 1781
BspTI	CTTAAG	1 cut(s)	1835
BspTNI	GGTCTC	2 cut(s)	378, 1677
BsrDI	GCAATG	2 cut(s)	985, 1428
BsrFI	RCCGGY	3 cut(s)	830, 1160, 1462
BsrI	ACTGG	1 cut(s)	1356
BssAI	RCCGGY	3 cut(s)	830, 1160, 1462
BssNI	GRCGYC	4 cut(s)	686, 858, 864, 1607
BssT1I	CCWWGG	5 cut(s)	107, 508, 670, 1117, 1432
Bst2UI	CCWGG	4 cut(s)	267, 416, 1763, 1818
BstACI	GRCGYC	4 cut(s)	686, 858, 864, 1607
BstAFI	CTTAAG	1 cut(s)	1835
BstAPI	GCANNNNNTGC	1 cut(s)	776
BstBAI	YACGTR	5 cut(s)	125, 1415, 1627, 1792, 2078
BstDEI	CTNAG	2 cut(s)	1552, 1800
BstDSI	CCRYGG	6 cut(s)	508, 670, 1019, 1117, 1704, 1952
BstF5I	GGATG	4 cut(s)	1139, 1197, 1849, 2023
BstFNI	CGCG	5 cut(s)	1092, 1486, 1725, 1999, 2203
BstH2I	RGCGCY	2 cut(s)	1068, 1538
BstHHI	GCGC	5 cut(s)	719, 814, 1067, 1428, 1537
BstMAI	GTCTC	4 cut(s)	378, 651, 1509, 1677
BstMWI	GCNNNNNNNGC	5 cut(s)	313, 767, 776, 935, 984
BstNI	CCWGG	4 cut(s)	267, 416, 1763, 1818
BstPAI	GACNNNNGTC	1 cut(s)	1010
BstSCI	CCNGG	6 cut(s)	265, 414, 553, 1537, 1761, 1816
BstSFI	CTRYAG	1 cut(s)	784
BstSLI	GKGCMC	2 cut(s)	989, 1660
BstSNI	TACGTA	1 cut(s)	2078
BstUI	CGCG	5 cut(s)	1092, 1486, 1725, 1999, 2203
BstV1I	GCAGC	5 cut(s)	507, 533, 1430, 1627, 1725
BstV2I	GAAGAC	1 cut(s)	1142
BstX2I	RGATCY	2 cut(s)	913, 1795
BstXI	CCANNNNNNTGG	1 cut(s)	509
BstYI	RGATCY	2 cut(s)	913, 1795
BtgI	CCRYGG	6 cut(s)	508, 670, 1019, 1117, 1704, 1952
BtgZI	GCGATG	2 cut(s)	1081, 1739
BtrI	CACGTC	1 cut(s)	709
BtsCI	GGATG	4 cut(s)	1139, 1197, 1849, 2023
BtsIMutI	CAGTG	2 cut(s)	255, 1349
BtuMI	TCGCGA	2 cut(s)	1486, 1725
CfoI	GCGC	5 cut(s)	719, 814, 1067, 1428, 1537
Cfr10I	RCCGGY	3 cut(s)	830, 1160, 1462
Cfr13I	GGNCC	8 cut(s)	518, 985, 986, 1437, 1490, 1656, 1657, 1733
CseI	GACGC	6 cut(s)	305, 675, 847, 1081, 1857, 2005
CsiI	ACCWGGT	1 cut(s)	1761
Csp6I	GTAC	5 cut(s)	196, 824, 848, 904, 2075
CspAI	ACCGGT	2 cut(s)	1160, 1462
CspCI	CAANNNNNGTGG	2 cut(s)	2053, 2088
CviAII	CATG	9 cut(s)	188, 509, 671, 1118, 1174, 1612, 1681, 1696, 2207
CviQI	GTAC	5 cut(s)	196, 824, 848, 904, 2075
DdeI	CTNAG	2 cut(s)	1552, 1800
DraIII	CACNNNGTG	2 cut(s)	128, 1765
DrdI	GACNNNNNNGTC	1 cut(s)	1612
DriI	GACNNNNNGTC	3 cut(s)	707, 779, 1480
DseDI	GACNNNNNNGTC	1 cut(s)	1612
EaeI	YGGCCR	7 cut(s)	482, 667, 1127, 1562, 1692, 2003, 2224
Eam1105I	GACNNNNNGTC	3 cut(s)	707, 779, 1480
EciI	GGCGGA	2 cut(s)	265, 1856
Eco105I	TACGTA	1 cut(s)	2078
Eco130I	CCWWGG	5 cut(s)	107, 508, 670, 1117, 1432
Eco24I	GRGCYC	2 cut(s)	989, 1660
Eco31I	GGTCTC	2 cut(s)	378, 1677
Eco47I	GGWCC	3 cut(s)	518, 1437, 1490
Eco57I	CTGAAG	2 cut(s)	660, 1278
Eco72I	CACGTG	3 cut(s)	1415, 1627, 1792
Eco88I	CYCGRG	1 cut(s)	618
EcoO109I	RGGNCCY	2 cut(s)	1490, 1656
EcoRI	GAATTC	1 cut(s)	420
EcoRII	CCWGG	4 cut(s)	265, 414, 1761, 1816
EcoT14I	CCWWGG	5 cut(s)	107, 508, 670, 1117, 1432
EcoT22I	ATGCAT	2 cut(s)	6, 1175
EcoT38I	GRGCYC	2 cut(s)	989, 1660
ErhI	CCWWGG	5 cut(s)	107, 508, 670, 1117, 1432
Esp3I	CGTCTC	1 cut(s)	651
FaeI	CATG	9 cut(s)	191, 512, 674, 1121, 1177, 1615, 1684, 1699, 2210
FalI	AAGNNNNNCTT	4 cut(s)	706, 738, 759, 791
FaqI	GGGAC	3 cut(s)	862, 1450, 1618
FatI	CATG	9 cut(s)	187, 508, 670, 1117, 1173, 1611, 1680, 1695, 2206
FauI	CCCGC	3 cut(s)	976, 1667, 1684
FauNDI	CATATG	1 cut(s)	479
FbaI	TGATCA	1 cut(s)	1683
FblI	GTMKAC	3 cut(s)	249, 783, 861
FokI	GGATG	4 cut(s)	1126, 1184, 1856, 2030
FriOI	GRGCYC	2 cut(s)	989, 1660
FspBI	CTAG	4 cut(s)	369, 654, 1665, 1742
FspI	TGCGCA	1 cut(s)	813
GlaI	GCGC	5 cut(s)	718, 813, 1066, 1427, 1536
GsaI	CCCAGC	1 cut(s)	1973
GsuI	CTGGAG	1 cut(s)	993
HaeII	RGCGCY	2 cut(s)	1068, 1538
HapII	CCGG	9 cut(s)	441, 555, 831, 1130, 1161, 1387, 1463, 1538, 1785
HgaI	GACGC	6 cut(s)	305, 675, 847, 1081, 1857, 2005
HhaI	GCGC	5 cut(s)	719, 814, 1067, 1428, 1537
Hin1I	GRCGYC	4 cut(s)	686, 858, 864, 1607
Hin1II	CATG	9 cut(s)	191, 512, 674, 1121, 1177, 1615, 1684, 1699, 2210
Hin6I	GCGC	5 cut(s)	717, 812, 1065, 1426, 1535
HinP1I	GCGC	5 cut(s)	717, 812, 1065, 1426, 1535
HincII	GTYRAC	3 cut(s)	690, 862, 1546
HindII	GTYRAC	3 cut(s)	690, 862, 1546
HindIII	AAGCTT	1 cut(s)	19
HinfI	GANTC	7 cut(s)	147, 233, 348, 437, 560, 890, 1408
HpaII	CCGG	9 cut(s)	441, 555, 831, 1130, 1161, 1387, 1463, 1538, 1785
HphI	GGTGA	9 cut(s)	82, 277, 607, 1018, 1393, 1462, 2063, 2256, 2287
Hpy166II	GTNNAC	7 cut(s)	250, 690, 784, 862, 1156, 1546, 2175
Hpy8I	GTNNAC	7 cut(s)	250, 690, 784, 862, 1156, 1546, 2175
HpyAV	CCTTC	2 cut(s)	537, 2048
HpyF10VI	GCNNNNNNNGC	5 cut(s)	313, 767, 776, 935, 984
HpyF3I	CTNAG	2 cut(s)	1552, 1800
Hsp92I	GRCGYC	4 cut(s)	686, 858, 864, 1607
Hsp92II	CATG	9 cut(s)	191, 512, 674, 1121, 1177, 1615, 1684, 1699, 2210
HspAI	GCGC	5 cut(s)	717, 812, 1065, 1426, 1535
Ksp22I	TGATCA	1 cut(s)	1683
LmnI	GCTCC	2 cut(s)	602, 1177
Lsp1109I	GCAGC	5 cut(s)	507, 533, 1430, 1627, 1725
LweI	GCATC	5 cut(s)	633, 728, 758, 1206, 1762
MabI	ACCWGGT	1 cut(s)	1761
MaeI	CTAG	4 cut(s)	369, 654, 1665, 1742
MboII	GAAGA	6 cut(s)	64, 639, 811, 1147, 1271, 2253
MflI	RGATCY	2 cut(s)	913, 1795
MhlI	GDGCHC	2 cut(s)	989, 1660
MlsI	TGGCCA	4 cut(s)	484, 669, 2005, 2226
MluCI	AATT	8 cut(s)	83, 420, 756, 1234, 1568, 1873, 2126, 2151
MluNI	TGGCCA	4 cut(s)	484, 669, 2005, 2226
MlyI	GAGTC	4 cut(s)	156, 227, 342, 884
MmeI	TCCRAC	2 cut(s)	691, 843
Mox20I	TGGCCA	4 cut(s)	484, 669, 2005, 2226
Mph1103I	ATGCAT	2 cut(s)	6, 1175
MscI	TGGCCA	4 cut(s)	484, 669, 2005, 2226
MseI	TTAA	1 cut(s)	1836
MslI	CAYNNNNRTG	3 cut(s)	507, 872, 2211
Msp20I	TGGCCA	4 cut(s)	484, 669, 2005, 2226
MspA1I	CMGCKG	4 cut(s)	474, 546, 1738, 2230
MspCI	CTTAAG	1 cut(s)	1835
MspI	CCGG	9 cut(s)	441, 555, 831, 1130, 1161, 1387, 1463, 1538, 1785
MspR9I	CCNGG	6 cut(s)	267, 416, 555, 1539, 1763, 1818
Mva1269I	GAATGC	2 cut(s)	380, 1285
MvaI	CCWGG	4 cut(s)	267, 416, 1763, 1818
MvnI	CGCG	5 cut(s)	1092, 1486, 1725, 1999, 2203
MwoI	GCNNNNNNNGC	5 cut(s)	313, 767, 776, 935, 984
NciI	CCSGG	2 cut(s)	555, 1539
NcoI	CCATGG	3 cut(s)	508, 670, 1117
NdeI	CATATG	1 cut(s)	479
NlaIII	CATG	9 cut(s)	191, 512, 674, 1121, 1177, 1615, 1684, 1699, 2210
NmeAIII	GCCGAG	3 cut(s)	366, 708, 861
NmuCI	GTSAC	9 cut(s)	88, 341, 1003, 1055, 1373, 1399, 1900, 1993, 2275
NruI	TCGCGA	2 cut(s)	1486, 1725
NsbI	TGCGCA	1 cut(s)	813
NsiI	ATGCAT	2 cut(s)	6, 1175
PcsI	WCGNNNNNNNCGW	7 cut(s)	408, 465, 834, 843, 861, 942, 1369
PctI	GAATGC	2 cut(s)	380, 1285
PfeI	GAWTC	3 cut(s)	437, 560, 1408
PflFI	GACNNNGTC	2 cut(s)	857, 1208
PflMI	CCANNNNNTGG	1 cut(s)	1765
PinAI	ACCGGT	2 cut(s)	1160, 1462
PleI	GAGTC	4 cut(s)	155, 227, 342, 884
PmaCI	CACGTG	3 cut(s)	1415, 1627, 1792
PmlI	CACGTG	3 cut(s)	1415, 1627, 1792
PpsI	GAGTC	4 cut(s)	155, 227, 342, 884
Ppu21I	YACGTR	5 cut(s)	125, 1415, 1627, 1792, 2078
PpuMI	RGGWCCY	1 cut(s)	1490
PshAI	GACNNNNGTC	1 cut(s)	1010
Psp5II	RGGWCCY	1 cut(s)	1490
Psp6I	CCWGG	4 cut(s)	265, 414, 1761, 1816
PspCI	CACGTG	3 cut(s)	1415, 1627, 1792
PspFI	CCCAGC	1 cut(s)	1969
PspGI	CCWGG	4 cut(s)	265, 414, 1761, 1816
PspOMI	GGGCCC	2 cut(s)	985, 1656
PspPI	GGNCC	8 cut(s)	518, 985, 986, 1437, 1490, 1656, 1657, 1733
PspPPI	RGGWCCY	1 cut(s)	1490
PsrI	GAACNNNNNNTAC	2 cut(s)	831, 863
PsuI	RGATCY	2 cut(s)	913, 1795
PsyI	GACNNNGTC	2 cut(s)	857, 1208
PvuII	CAGCTG	1 cut(s)	2230
RruI	TCGCGA	2 cut(s)	1486, 1725
RsaI	GTAC	5 cut(s)	197, 825, 849, 905, 2076
RsaNI	GTAC	5 cut(s)	196, 824, 848, 904, 2075
RseI	CAYNNNNRTG	3 cut(s)	507, 872, 2211
SalI	GTCGAC	1 cut(s)	860
SaqAI	TTAA	1 cut(s)	1836
Sau96I	GGNCC	8 cut(s)	518, 985, 986, 1437, 1490, 1656, 1657, 1733
SchI	GAGTC	4 cut(s)	156, 227, 342, 884
ScrFI	CCNGG	6 cut(s)	267, 416, 555, 1539, 1763, 1818
SduI	GDGCHC	2 cut(s)	989, 1660
SexAI	ACCWGGT	1 cut(s)	1761
SfaNI	GCATC	5 cut(s)	633, 728, 758, 1206, 1762
SfcI	CTRYAG	1 cut(s)	784
SgrAI	CRCCGGYG	2 cut(s)	1160, 1462
SgrDI	CGTCGACG	1 cut(s)	860
SinI	GGWCC	3 cut(s)	518, 1437, 1490
SmiMI	CAYNNNNRTG	3 cut(s)	507, 872, 2211
SmlI	CTYRAG	1 cut(s)	1835
SmoI	CTYRAG	1 cut(s)	1835
SnaBI	TACGTA	1 cut(s)	2078
Sse9I	AATT	8 cut(s)	83, 420, 756, 1234, 1568, 1873, 2126, 2151
SspMI	CTAG	4 cut(s)	369, 654, 1665, 1742
StyD4I	CCNGG	6 cut(s)	265, 414, 553, 1537, 1761, 1816
StyI	CCWWGG	5 cut(s)	107, 508, 670, 1117, 1432
TaqI	TCGA	6 cut(s)	468, 837, 861, 1266, 1572, 1871
TasI	AATT	8 cut(s)	83, 420, 756, 1234, 1568, 1873, 2126, 2151
TfiI	GAWTC	3 cut(s)	437, 560, 1408
Tru1I	TTAA	1 cut(s)	1836
Tru9I	TTAA	1 cut(s)	1836
TscAI	CASTG	2 cut(s)	262, 1356
TseFI	GTSAC	9 cut(s)	88, 341, 1003, 1055, 1373, 1399, 1900, 1993, 2275
TseI	GCWGC	5 cut(s)	495, 546, 1418, 1640, 1738
Tsp45I	GTSAC	9 cut(s)	88, 341, 1003, 1055, 1373, 1399, 1900, 1993, 2275
TspDTI	ATGAA	4 cut(s)	127, 204, 1409, 1899
TspGWI	ACGGA	4 cut(s)	329, 1497, 1580, 1743
TspRI	CASTG	2 cut(s)	262, 1356
Tth111I	GACNNNGTC	2 cut(s)	857, 1208
Van91I	CCANNNNNTGG	1 cut(s)	1765
Vha464I	CTTAAG	1 cut(s)	1835
VpaK11BI	GGWCC	3 cut(s)	518, 1437, 1490
XapI	RAATTY	3 cut(s)	83, 420, 2151
XmiI	GTMKAC	3 cut(s)	249, 783, 861
XspI	CTAG	4 cut(s)	369, 654, 1665, 1742
ZraI	GACGTC	2 cut(s)	865, 1608
Zsp2I	ATGCAT	2 cut(s)	6, 1175

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.