MD00G1118600.v1.1

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0013239
Physical Location & Seq
Forward (+)
25336856 .. 25341355
4500 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1118600.v1.1.491

Sequence Viewer

Length: 1452 bp
ATGGAAGGGCCTTCGACGGCCGGCGGAGGCGGTGGCCCGGCGCCGTTTCTGCTGAAGACGTACGACATGGTGGACGATTCCGCGACCGACGAGATCGTGTCGTGGAGCTCGAATAAGAAAAGCTTCATCGTCTGGAACCCGCCCGAGTTTGCTCGGGTTCTGCTCCCCACCTATTTCAAGCACAACAACTTCTCCAGCTTCATTCGTCAGCTCAATACATACGGATTTCGAAAGATTGATCCTGAGAGATGGGAATTTGCTAATGAAGACTTCATACAAGATCAAAAGCATCTTCTTAAGAATATCCACCGCAGAAAACCCATTCACAGCCACAGCAATCCTCAAGGTTCTATGGTTGATTCAGAAAGAGCAGCTCTTGATGACGAAATAGAGAAGCTTTCACATGATAAAGCTGCACTCGAGGCAAATATTTCAAGGTTCAAGCAGCAGCGATCTGCGGCAAAGCTTCAGTTGGAAGACTTAACACAACGGGTGAATGGTATGGAACAACGGCAGAAGAATTTGGTGACATTCTTAGATAGGGCTGTTCACAACCCTAATTTTGTTGAACATCTCACTAGAAAAATTGAATCTATGGATTTCTCAGCATATCATAAGAAAAGGCGATTGCCTGACATTGACCATCTACAGCCAGTCGTGGAGAATGGTTTTGTGGATAACCGAAGTAGTTCTAGATCTGAGTTTGGAAATATTTTCCACCAAGACTTCTCAAGTAAACTTAGACTTGAATTATCACCAGCTGTTTCAGACATGAATTTGGTATCACGTAGCACGCAGAGTTCCAATGAAGATGGGGGTAGTTCAACTAGGAAAATATCTGAAGAACTTAAAGGTGCACAGATGAGACCAGAAGGCGTTTTATTTGCACCTGAAACCTTAGAACTTTCAGATACTGGAACATCTTTTGCATTCAAAATGGATTCGTTGTTATCGCGGAAAGCACCAACTGTTGGAAGCCCTAGACGTCATTCCTTGCAGCCAGGTTTGCCTTCTAATGAAGAAGGTGATGGCCATATATCCTGCCACTTAAATCTCACTTTAGCATCTACTCCGTTGCAAGTTAATAATAGTCCTTGTTTAGCTACAGTACCCCAAGTAGGTCAGGATATCAACGAATCTGCAGCATCAGAGCTAAATGAAAATGGAAAAGATTCGGATTTAAGAGCCTTTACAAACAAAAATATAGCTGACAACGACAAACTCAAAACTTCCTCCCAAGAAGCCACACATAACAATCAAGCGCCCCCACTGGTTCCAGTTAGAGTAAACGATGTCTTCTGGGAACAGTTCCTAACTGAAAGACCTGGCTGTTCAGAGAATGAAGAAGCAAGTTCTGATTATAGGGGAAATCCATTTGATGAGCAAGATGATGGAAGGTTAGACCATGGAATGTCCAGAAATGTCGAGGATGCGGAAACACTCACTCTTTGA

Protein Analysis

484

Amino Acids

54.18

Weight (kDa)

5.45

Isoelectric Point (pI)

61.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 16 - 105 5.6e-29 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013239)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13980
fragaria_vesca FvH4_4g13230
malus_domestica MD00G1118600.v1.1 MD04G1017400.v1.1
prunus_persica Prupe.1G165500_v2.0.a1
pyrus_communis pycom04g01380 pycom420g00940
rosa_chinensis RchiOBHm_Chr4g0413481
rosa_laevigata RLG00000008216
rosa_multiflora Rmu_sc0000883.1_g000011
rosa_roxburghii Rroxscaffold_5G00357380
rosa_rugosa Rorug04G0120600
rosa_samantha Rh4AG180300 Rh4BG179600 Rh4CG191300 Rh4DG175500
rosa_wichuraiana Rw4G015010 Rw4G015070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 988
AccB1I GGYRCC 1 cut(s) 40
AccB7I CCANNNNNTGG 1 cut(s) 971
AccII CGCG 2 cut(s) 83, 955
AciI CCGC 8 cut(s) 24, 30, 81, 140, 310, 458, 955, 1433
AclWI GGATC 1 cut(s) 233
AcoI YGGCCR 2 cut(s) 18, 1030
AcsI RAATTY 3 cut(s) 254, 520, 775
AcuI CTGAAG 3 cut(s) 74, 452, 861
AcyI GRCGYC 2 cut(s) 41, 985
AfaI GTAC 2 cut(s) 62, 1110
AfiI CCNNNNNNNGG 2 cut(s) 971, 1118
AflII CTTAAG 1 cut(s) 296
AgsI TTSAA 8 cut(s) 178, 435, 442, 569, 590, 749, 825, 934
AjnI CCWGG 2 cut(s) 1000, 1324
Alw21I GWGCWC 2 cut(s) 110, 859
Alw26I GTCTC 1 cut(s) 859
Alw44I GTGCAC 1 cut(s) 855
AlwI GGATC 1 cut(s) 233
AlwNI CAGNNNCTG 1 cut(s) 914
Ama87I CYCGRG 3 cut(s) 143, 153, 419
AoxI GGCC 4 cut(s) 8, 18, 34, 1030
ApaLI GTGCAC 1 cut(s) 855
ApeKI GCWGC 6 cut(s) 371, 413, 445, 448, 997, 1142
ApoI RAATTY 3 cut(s) 254, 520, 775
Asp700I GAANNNNTTC 3 cut(s) 122, 688, 1171
AspLEI GCGC 2 cut(s) 43, 1264
AspS9I GGNCC 2 cut(s) 8, 35
AsuC2I CCSGG 1 cut(s) 38
AsuHPI GGTGA 4 cut(s) 505, 538, 747, 1037
AsuII TTCGAA 1 cut(s) 229
AvaI CYCGRG 3 cut(s) 143, 153, 419
BaeGI GKGCMC 1 cut(s) 859
BalI TGGCCA 1 cut(s) 1032
BanI GGYRCC 1 cut(s) 40
BanII GRGCYC 1 cut(s) 110
BarI GAAGNNNNNNTAC 2 cut(s) 258, 290
BbsI GAAGAC 4 cut(s) 62, 273, 483, 1288
Bbv12I GWGCWC 2 cut(s) 110, 859
BbvI GCAGC 6 cut(s) 383, 400, 457, 460, 1009, 1154
BccI CCATC 5 cut(s) 243, 651, 806, 1022, 1385
BceAI ACGGC 3 cut(s) 28, 33, 527
BciT130I CCWGG 2 cut(s) 1002, 1326
BcnI CCSGG 1 cut(s) 38
BcoDI GTCTC 1 cut(s) 859
BfaI CTAG 4 cut(s) 579, 693, 828, 981
BfmI CTRYAG 3 cut(s) 647, 1104, 1140
BfoI RGCGCY 2 cut(s) 44, 1265
BfrI CTTAAG 1 cut(s) 296
BglII AGATCT 1 cut(s) 695
BisI GCNGC 7 cut(s) 372, 414, 446, 449, 459, 998, 1143
BlsI GCNGC 7 cut(s) 373, 415, 447, 450, 460, 999, 1144
Bme1390I CCNGG 3 cut(s) 38, 1002, 1326
BmeT110I CYCGRG 3 cut(s) 143, 153, 419
BmgT120I GGNCC 2 cut(s) 8, 35
BmiI GGNNCC 3 cut(s) 42, 137, 1275
BmrFI CCNGG 3 cut(s) 38, 1002, 1326
BmsI GCATC 4 cut(s) 298, 1073, 1154, 1420
BpiI GAAGAC 4 cut(s) 62, 273, 483, 1288
BpmI CTGGAG 1 cut(s) 178
Bpu14I TTCGAA 1 cut(s) 229
BpuEI CTTGAG 2 cut(s) 327, 715
BpuMI CCSGG 1 cut(s) 38
BsaAI YACGTR 1 cut(s) 788
BsaHI GRCGYC 2 cut(s) 41, 985
BsaI GGTCTC 1 cut(s) 859
BsaJI CCNNGG 1 cut(s) 1405
BsaXI ACNNNNNCTCC 2 cut(s) 176, 206
Bsc4I CCNNNNNNNGG 2 cut(s) 971, 1118
Bse118I RCCGGY 1 cut(s) 20
Bse1I ACTGG 4 cut(s) 653, 919, 1275, 1277
BseBI CCWGG 2 cut(s) 1002, 1326
BseDI CCNNGG 1 cut(s) 1405
BseGI GGATG 1 cut(s) 1435
BseLI CCNNNNNNNGG 2 cut(s) 971, 1118
BseMII CTCAG 3 cut(s) 234, 618, 690
BseNI ACTGG 4 cut(s) 653, 919, 1275, 1277
BseSI GKGCMC 1 cut(s) 859
BseX3I CGGCCG 1 cut(s) 18
BseXI GCAGC 6 cut(s) 383, 400, 457, 460, 1009, 1154
BsgI GTGCAG 1 cut(s) 399
Bsh1236I CGCG 2 cut(s) 83, 955
Bsh1285I CGRYCG 2 cut(s) 21, 87
BshFI GGCC 4 cut(s) 10, 20, 36, 1032
BshNI GGYRCC 1 cut(s) 40
BsiEI CGRYCG 2 cut(s) 21, 87
BsiHKAI GWGCWC 2 cut(s) 110, 859
BsiHKCI CYCGRG 3 cut(s) 143, 153, 419
BsiSI CCGG 2 cut(s) 21, 38
BsiWI CGTACG 1 cut(s) 60
BslI CCNNNNNNNGG 2 cut(s) 971, 1118
BsmAI GTCTC 1 cut(s) 859
BsmI GAATGC 1 cut(s) 929
BsnI GGCC 4 cut(s) 10, 20, 36, 1032
Bso31I GGTCTC 1 cut(s) 859
BsoBI CYCGRG 3 cut(s) 143, 153, 419
Bsp119I TTCGAA 1 cut(s) 229
Bsp1286I GDGCHC 2 cut(s) 110, 859
Bsp143I GATC 5 cut(s) 93, 238, 280, 452, 695
Bsp19I CCATGG 1 cut(s) 1405
BspACI CCGC 8 cut(s) 24, 30, 81, 140, 310, 458, 955, 1433
BspANI GGCC 4 cut(s) 10, 20, 36, 1032
BspCNI CTCAG 3 cut(s) 235, 617, 691
BspFNI CGCG 2 cut(s) 83, 955
BspLI GGNNCC 3 cut(s) 42, 137, 1275
BspMAI CTGCAG 1 cut(s) 1144
BspPI GGATC 1 cut(s) 233
BspT104I TTCGAA 1 cut(s) 229
BspT107I GGYRCC 1 cut(s) 40
BspTI CTTAAG 1 cut(s) 296
BspTNI GGTCTC 1 cut(s) 859
BsrFI RCCGGY 1 cut(s) 20
BsrI ACTGG 4 cut(s) 653, 919, 1275, 1277
BssAI RCCGGY 1 cut(s) 20
BssECI CCNNGG 1 cut(s) 1405
BssMI GATC 5 cut(s) 93, 238, 280, 452, 695
BssNI GRCGYC 2 cut(s) 41, 985
BssT1I CCWWGG 1 cut(s) 1405
Bst2UI CCWGG 2 cut(s) 1002, 1326
Bst4CI ACNGT 3 cut(s) 970, 1108, 1308
BstACI GRCGYC 2 cut(s) 41, 985
BstAFI CTTAAG 1 cut(s) 296
BstBAI YACGTR 1 cut(s) 788
BstBI TTCGAA 1 cut(s) 229
BstC8I GCNNGC 2 cut(s) 22, 794
BstDEI CTNAG 6 cut(s) 243, 535, 604, 699, 740, 898
BstDSI CCRYGG 1 cut(s) 1405
BstF5I GGATG 1 cut(s) 1435
BstFNI CGCG 2 cut(s) 83, 955
BstH2I RGCGCY 2 cut(s) 44, 1265
BstHHI GCGC 2 cut(s) 43, 1264
BstKTI GATC 5 cut(s) 96, 241, 283, 455, 698
BstMAI GTCTC 1 cut(s) 859
BstMBI GATC 5 cut(s) 93, 238, 280, 452, 695
BstMCI CGRYCG 2 cut(s) 21, 87
BstMWI GCNNNNNNNGC 3 cut(s) 49, 422, 1006
BstNI CCWGG 2 cut(s) 1002, 1326
BstSCI CCNGG 3 cut(s) 36, 1000, 1324
BstSFI CTRYAG 3 cut(s) 647, 1104, 1140
BstSLI GKGCMC 1 cut(s) 859
BstUI CGCG 2 cut(s) 83, 955
BstV1I GCAGC 6 cut(s) 383, 400, 457, 460, 1009, 1154
BstV2I GAAGAC 4 cut(s) 62, 273, 483, 1288
BstX2I RGATCY 1 cut(s) 695
BstYI RGATCY 1 cut(s) 695
BstZI CGGCCG 1 cut(s) 18
BsuRI GGCC 4 cut(s) 10, 20, 36, 1032
BtgI CCRYGG 1 cut(s) 1405
BtsCI GGATG 1 cut(s) 1435
BtsIMutI CAGTG 1 cut(s) 1268
Cac8I GCNNGC 2 cut(s) 22, 794
CaiI CAGNNNCTG 1 cut(s) 914
CfoI GCGC 2 cut(s) 43, 1264
Cfr10I RCCGGY 1 cut(s) 20
Cfr13I GGNCC 2 cut(s) 8, 35
Csp6I GTAC 2 cut(s) 61, 1109
CviAII CATG 4 cut(s) 67, 404, 772, 1406
CviQI GTAC 2 cut(s) 61, 1109
DdeI CTNAG 6 cut(s) 243, 535, 604, 699, 740, 898
DinI GGCGCC 1 cut(s) 42
DpnI GATC 5 cut(s) 95, 240, 282, 454, 697
DpnII GATC 5 cut(s) 93, 238, 280, 452, 695
EaeI YGGCCR 2 cut(s) 18, 1030
EagI CGGCCG 1 cut(s) 18
EciI GGCGGA 1 cut(s) 39
Ecl136II GAGCTC 1 cut(s) 108
EclXI CGGCCG 1 cut(s) 18
Eco130I CCWWGG 1 cut(s) 1405
Eco24I GRGCYC 1 cut(s) 110
Eco31I GGTCTC 1 cut(s) 859
Eco32I GATATC 1 cut(s) 1129
Eco52I CGGCCG 1 cut(s) 18
Eco53kI GAGCTC 1 cut(s) 108
Eco57I CTGAAG 3 cut(s) 74, 452, 861
Eco88I CYCGRG 3 cut(s) 143, 153, 419
EcoICRI GAGCTC 1 cut(s) 108
EcoO109I RGGNCCY 1 cut(s) 8
EcoRII CCWGG 2 cut(s) 1000, 1324
EcoRV GATATC 1 cut(s) 1129
EcoT14I CCWWGG 1 cut(s) 1405
EcoT38I GRGCYC 1 cut(s) 110
EgeI GGCGCC 1 cut(s) 42
EheI GGCGCC 1 cut(s) 42
ErhI CCWWGG 1 cut(s) 1405
FaeI CATG 4 cut(s) 70, 407, 775, 1409
FalI AAGNNNNNCTT 2 cut(s) 107, 139
FatI CATG 4 cut(s) 66, 403, 771, 1405
FauI CCCGC 1 cut(s) 147
Fnu4HI GCNGC 7 cut(s) 372, 414, 446, 449, 459, 998, 1143
FokI GGATG 1 cut(s) 1442
FriOI GRGCYC 1 cut(s) 110
Fsp4HI GCNGC 7 cut(s) 372, 414, 446, 449, 459, 998, 1143
FspBI CTAG 4 cut(s) 579, 693, 828, 981
GlaI GCGC 2 cut(s) 42, 1263
GluI GCNGC 7 cut(s) 372, 414, 446, 449, 459, 998, 1143
GsuI CTGGAG 1 cut(s) 178
HaeII RGCGCY 2 cut(s) 44, 1265
HaeIII GGCC 4 cut(s) 10, 20, 36, 1032
HapII CCGG 2 cut(s) 21, 38
HhaI GCGC 2 cut(s) 43, 1264
Hin1I GRCGYC 2 cut(s) 41, 985
Hin1II CATG 4 cut(s) 70, 407, 775, 1409
Hin6I GCGC 2 cut(s) 41, 1262
HinP1I GCGC 2 cut(s) 41, 1262
HindIII AAGCTT 3 cut(s) 121, 395, 464
HinfI GANTC 6 cut(s) 77, 359, 590, 941, 1136, 1172
HpaII CCGG 2 cut(s) 21, 38
HphI GGTGA 4 cut(s) 505, 538, 747, 1037
Hpy166II GTNNAC 5 cut(s) 73, 550, 737, 857, 1288
Hpy188I TCNGA 9 cut(s) 364, 700, 769, 841, 910, 1150, 1177, 1336, 1357
Hpy188III TCNNGA 6 cut(s) 133, 242, 377, 693, 1124, 1416
Hpy8I GTNNAC 5 cut(s) 73, 550, 737, 857, 1288
Hpy99I CGWCG 2 cut(s) 19, 92
HpyAV CCTTC 5 cut(s) 21, 866, 1016, 1020, 1389
HpyCH4III ACNGT 3 cut(s) 970, 1108, 1308
HpyCH4IV ACGT 3 cut(s) 59, 787, 985
HpyCH4V TGCA 7 cut(s) 416, 857, 887, 929, 997, 1078, 1142
HpyF10VI GCNNNNNNNGC 3 cut(s) 49, 422, 1006
HpyF3I CTNAG 6 cut(s) 243, 535, 604, 699, 740, 898
HpySE526I ACGT 3 cut(s) 59, 787, 985
Hsp92I GRCGYC 2 cut(s) 41, 985
Hsp92II CATG 4 cut(s) 70, 407, 775, 1409
HspAI GCGC 2 cut(s) 41, 1262
KasI GGCGCC 1 cut(s) 40
KroI GCCGGC 1 cut(s) 20
KroNI GCCGGC 1 cut(s) 22
Kzo9I GATC 5 cut(s) 93, 238, 280, 452, 695
LmnI GCTCC 2 cut(s) 105, 168
Lsp1109I GCAGC 6 cut(s) 383, 400, 457, 460, 1009, 1154
LweI GCATC 4 cut(s) 298, 1073, 1154, 1420
MaeI CTAG 4 cut(s) 579, 693, 828, 981
MaeII ACGT 3 cut(s) 59, 787, 985
MaeIII GTNAC 1 cut(s) 526
MalI GATC 5 cut(s) 95, 240, 282, 454, 697
MboI GATC 5 cut(s) 93, 238, 280, 452, 695
MflI RGATCY 1 cut(s) 695
MhlI GDGCHC 2 cut(s) 110, 859
MlsI TGGCCA 1 cut(s) 1032
MluCI AATT 6 cut(s) 254, 520, 559, 585, 749, 775
MluNI TGGCCA 1 cut(s) 1032
Mly113I GGCGCC 1 cut(s) 41
MmeI TCCRAC 2 cut(s) 453, 952
MnlI CCTC 5 cut(s) 20, 351, 415, 1243, 1420
Mox20I TGGCCA 1 cut(s) 1032
MroNI GCCGGC 1 cut(s) 20
MroXI GAANNNNTTC 3 cut(s) 122, 688, 1171
MscI TGGCCA 1 cut(s) 1032
MseI TTAA 6 cut(s) 297, 482, 849, 1049, 1083, 1181
Msp20I TGGCCA 1 cut(s) 1032
MspA1I CMGCKG 1 cut(s) 761
MspCI CTTAAG 1 cut(s) 296
MspI CCGG 2 cut(s) 21, 38
MspR9I CCNGG 3 cut(s) 38, 1002, 1326
Mva1269I GAATGC 1 cut(s) 929
MvaI CCWGG 2 cut(s) 1002, 1326
MvnI CGCG 2 cut(s) 83, 955
MwoI GCNNNNNNNGC 3 cut(s) 49, 422, 1006
NaeI GCCGGC 1 cut(s) 22
NarI GGCGCC 1 cut(s) 41
NciI CCSGG 1 cut(s) 38
NcoI CCATGG 1 cut(s) 1405
NdeII GATC 5 cut(s) 93, 238, 280, 452, 695
NgoMIV GCCGGC 1 cut(s) 20
NlaIII CATG 4 cut(s) 70, 407, 775, 1409
NlaIV GGNNCC 3 cut(s) 42, 137, 1275
NmuCI GTSAC 1 cut(s) 526
NspV TTCGAA 1 cut(s) 229
PaeR7I CTCGAG 1 cut(s) 419
PcsI WCGNNNNNNNCGW 1 cut(s) 107
PctI GAATGC 1 cut(s) 929
PdiI GCCGGC 1 cut(s) 22
PdmI GAANNNNTTC 3 cut(s) 122, 688, 1171
PfeI GAWTC 6 cut(s) 77, 359, 590, 941, 1136, 1172
Pfl23II CGTACG 1 cut(s) 60
PflMI CCANNNNNTGG 1 cut(s) 971
PkrI GCNGC 7 cut(s) 373, 415, 447, 450, 460, 999, 1144
PluTI GGCGCC 1 cut(s) 44
Ppu21I YACGTR 1 cut(s) 788
Psp124BI GAGCTC 1 cut(s) 110
Psp6I CCWGG 2 cut(s) 1000, 1324
PspGI CCWGG 2 cut(s) 1000, 1324
PspLI CGTACG 1 cut(s) 60
PspN4I GGNNCC 3 cut(s) 42, 137, 1275
PspPI GGNCC 2 cut(s) 8, 35
PspXI VCTCGAGB 1 cut(s) 419
PstI CTGCAG 1 cut(s) 1144
PstNI CAGNNNCTG 1 cut(s) 914
PsuI RGATCY 1 cut(s) 695
PvuII CAGCTG 1 cut(s) 761
RsaI GTAC 2 cut(s) 62, 1110
RsaNI GTAC 2 cut(s) 61, 1109
SacI GAGCTC 1 cut(s) 110
SaqAI TTAA 6 cut(s) 297, 482, 849, 1049, 1083, 1181
SatI GCNGC 7 cut(s) 372, 414, 446, 449, 459, 998, 1143
Sau3AI GATC 5 cut(s) 93, 238, 280, 452, 695
Sau96I GGNCC 2 cut(s) 8, 35
ScrFI CCNGG 3 cut(s) 38, 1002, 1326
SduI GDGCHC 2 cut(s) 110, 859
SfaNI GCATC 4 cut(s) 298, 1073, 1154, 1420
SfcI CTRYAG 3 cut(s) 647, 1104, 1140
SfoI GGCGCC 1 cut(s) 42
Sfr274I CTCGAG 1 cut(s) 419
SfuI TTCGAA 1 cut(s) 229
SlaI CTCGAG 1 cut(s) 419
SmlI CTYRAG 4 cut(s) 296, 342, 419, 730
SmoI CTYRAG 4 cut(s) 296, 342, 419, 730
Sse9I AATT 6 cut(s) 254, 520, 559, 585, 749, 775
SsiI CCGC 8 cut(s) 24, 30, 81, 140, 310, 458, 955, 1433
SspDI GGCGCC 1 cut(s) 40
SspI AATATT 2 cut(s) 430, 712
SspMI CTAG 4 cut(s) 579, 693, 828, 981
SstI GAGCTC 1 cut(s) 110
StyD4I CCNGG 3 cut(s) 36, 1000, 1324
StyI CCWWGG 1 cut(s) 1405
TaaI ACNGT 3 cut(s) 970, 1108, 1308
TaiI ACGT 3 cut(s) 62, 790, 988
TaqI TCGA 5 cut(s) 14, 110, 229, 420, 1425
TaqII GACCGA 1 cut(s) 101
TasI AATT 6 cut(s) 254, 520, 559, 585, 749, 775
TauI GCSGC 1 cut(s) 461
TfiI GAWTC 6 cut(s) 77, 359, 590, 941, 1136, 1172
Tru1I TTAA 6 cut(s) 297, 482, 849, 1049, 1083, 1181
Tru9I TTAA 6 cut(s) 297, 482, 849, 1049, 1083, 1181
TscAI CASTG 1 cut(s) 1275
TseFI GTSAC 1 cut(s) 526
TseI GCWGC 6 cut(s) 371, 413, 445, 448, 997, 1142
Tsp45I GTSAC 1 cut(s) 526
TspDTI ATGAA 9 cut(s) 115, 190, 262, 279, 788, 822, 1032, 1173, 1356
TspGWI ACGGA 2 cut(s) 237, 1062
TspRI CASTG 1 cut(s) 1275
Van91I CCANNNNNTGG 1 cut(s) 971
Vha464I CTTAAG 1 cut(s) 296
VneI GTGCAC 1 cut(s) 855
XapI RAATTY 3 cut(s) 254, 520, 775
XbaI TCTAGA 1 cut(s) 692
XhoI CTCGAG 1 cut(s) 419
XmnI GAANNNNTTC 3 cut(s) 122, 688, 1171
XspI CTAG 4 cut(s) 579, 693, 828, 981
ZraI GACGTC 1 cut(s) 986
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.