Rroxscaffold_5G00357380

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0013239
Physical Location & Seq
Forward (+)
36992981 .. 36996705
3725 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00357380.1

Sequence Viewer

Length: 1431 bp
ATGGACGGAACCTCTGCTGCTGGCGGAGGCGGCGGTCCGGCGCCCTTCTTACTGAAGACGTACGACATGGTGGACGACTCGGCGACGGACGAGATCGTGTCGTGGAACTCCAACAAGAAGAGCTTCGTCGTCTGGAACCCTCCAGAATTCGCTCGGCTTCTGCTCCCCACCTATTTCAAGCACAACAACTTCTCTAGCTTCATCCGTCAGCTCAATACCTACGGATTTCGAAAGATTGATCCTGAAAGATGGGAGTTTGCTAATGAAGAATTTATACAAGATCAAAAGCATCTTCTTAAGAATATTCATCGCAGAAAACCCATTCATAGCCACAGTAATCCTCAGGGTTCTATGGTAGATCAGGAAAGAGCAGCTCTTGATGATGAAATAGAGAAGCTATCAAACGAAAAAGCTGCACTTGAGGCAAATATTTCAAGGTTCCAGCAGCAGCGATCTGCTGAAAAGCTTCAGTTGGAAGACCTGACACAACGGGTAAATGGTATGGAGCAACGGCAGAAGAATTTACTAACATTCTTAGATAAAGCTGTTCAGGACCCTGCCTTTGTTGAACATCTAGCCCGAAAAATTGAATCTATGGATTTCTCAGCATATAACAAGAAAAGGCGACTGCCTGATGCGGACAACTCACAGCCAGTTGCGGACAATAGTTTTGTGGATAACCAAAGTAGCTCTAGATCGGAGTTTGGAAATTTTTTCCACCAAGACTTTTCAAGTAAGCTGAGACTGGAATTATCTCCAGCTGTTTCAGACATGAACCTGGTTTCACGTAGCACCCAGAGTTCCAATGAAGATGGGGATAGTTCAAGTAGGAAAATATCGGAAGAACTGAAAGGTGCACAGATGAGAACAGAAGGCCTTTTATTTGCAAATGAACCACTAGAACTTTCAGATACTGGAACATCTTTTGCATTCAAAATGGATTCGTTGTCACGAAAAGCACCAAATGTTGGAAGCCCTGGACTTCATTCCTTGCAGCCAGGTTTGCCTTCTAATGAAGATAGTGATGGCCAGATAACCTGTCACTTAAATCTCACACTATCATCTTCTCCCTTGAAAGTGATCAGTAGCCCACATTCAGCTATGGTACCCCTAGTAGGTCAGGACATTTGCAACTCCCCATCCCCAGGTTTGAATGCCACTAACAAAGAATCAGATATAAGAGCCCTTCCACAGAAAAATCTGGCTGATGAAGACAGGCCTAAAAGTTCCCAAAAAGAGGCCACAAAACACAGTAAAGGGCATACATCTGTTCCAGTTAGAGTAAATGATGTTTTCTGGGAACAGTTCCTAACGGAAAGACCTGGTTGTTCAGAGAATGAAGAAGCGAGTTCTAATTTTAGAGAAAATCCGTATGATGAGCAAGATGGAATGCCCAGAAATGCTAAAGGTGCAGAAACACTTACTCTTTGA

Protein Analysis

476

Amino Acids

53.18

Weight (kDa)

5.44

Isoelectric Point (pI)

59.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 16 - 105 6.2e-29 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013239)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13980
fragaria_vesca FvH4_4g13230
malus_domestica MD00G1118600.v1.1 MD04G1017400.v1.1
prunus_persica Prupe.1G165500_v2.0.a1
pyrus_communis pycom04g01380 pycom420g00940
rosa_chinensis RchiOBHm_Chr4g0413481
rosa_laevigata RLG00000008216
rosa_multiflora Rmu_sc0000883.1_g000011
rosa_roxburghii Rroxscaffold_5G00357380
rosa_rugosa Rorug04G0120600
rosa_samantha Rh4AG180300 Rh4BG179600 Rh4CG191300 Rh4DG175500
rosa_wichuraiana Rw4G015010 Rw4G015070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1105
AccB1I GGYRCC 2 cut(s) 40, 1105
AccB7I CCANNNNNTGG 1 cut(s) 968
AciI CCGC 5 cut(s) 24, 30, 33, 638, 659
AclWI GGATC 1 cut(s) 233
AcoI YGGCCR 1 cut(s) 1027
AcsI RAATTY 4 cut(s) 146, 269, 520, 709
AcuI CTGAAG 2 cut(s) 74, 452
AcyI GRCGYC 1 cut(s) 41
AfaI GTAC 2 cut(s) 62, 1107
AfiI CCNNNNNNNGG 4 cut(s) 968, 1115, 1145, 1237
AflII CTTAAG 1 cut(s) 296
AgsI TTSAA 9 cut(s) 178, 435, 569, 590, 732, 825, 934, 1075, 1153
AjnI CCWGG 5 cut(s) 777, 976, 997, 1144, 1321
Alw21I GWGCWC 1 cut(s) 859
Alw26I GTCTC 1 cut(s) 736
Alw44I GTGCAC 1 cut(s) 855
AlwI GGATC 1 cut(s) 233
AlwNI CAGNNNCTG 1 cut(s) 914
AoxI GGCC 4 cut(s) 874, 1027, 1217, 1239
ApaLI GTGCAC 1 cut(s) 855
ApeKI GCWGC 6 cut(s) 17, 371, 413, 445, 448, 994
ApoI RAATTY 4 cut(s) 146, 269, 520, 709
ArsI GACNNNNNNTTYG 4 cut(s) 545, 577, 653, 685
Asp700I GAANNNNTTC 2 cut(s) 122, 465
Asp718I GGTACC 1 cut(s) 1105
AspLEI GCGC 1 cut(s) 43
AspS9I GGNCC 2 cut(s) 35, 553
AsuII TTCGAA 1 cut(s) 229
AvaII GGWCC 2 cut(s) 35, 553
AxyI CCTNAGG 1 cut(s) 342
BaeGI GKGCMC 1 cut(s) 859
BalI TGGCCA 1 cut(s) 1029
BanI GGYRCC 2 cut(s) 40, 1105
BanII GRGCYC 1 cut(s) 1186
BarI GAAGNNNNNNTAC 2 cut(s) 258, 290
BbsI GAAGAC 3 cut(s) 62, 483, 1218
Bbv12I GWGCWC 1 cut(s) 859
BbvI GCAGC 6 cut(s) 4, 383, 400, 457, 460, 1006
BccI CCATC 5 cut(s) 243, 806, 1019, 1147, 1379
BceAI ACGGC 1 cut(s) 527
BcgI CGANNNNNNTGC 2 cut(s) 395, 429
BciT130I CCWGG 5 cut(s) 779, 978, 999, 1146, 1323
BclI TGATCA 1 cut(s) 1080
BcoDI GTCTC 1 cut(s) 736
BfaI CTAG 5 cut(s) 195, 575, 693, 899, 1112
BfoI RGCGCY 1 cut(s) 44
BfrI CTTAAG 1 cut(s) 296
BisI GCNGC 7 cut(s) 18, 31, 372, 414, 446, 449, 995
BlsI GCNGC 7 cut(s) 19, 32, 373, 415, 447, 450, 996
Bme1390I CCNGG 5 cut(s) 779, 978, 999, 1146, 1323
Bme18I GGWCC 2 cut(s) 35, 553
BmgT120I GGNCC 2 cut(s) 35, 553
BmiI GGNNCC 6 cut(s) 10, 42, 137, 440, 555, 1107
BmrFI CCNGG 5 cut(s) 779, 978, 999, 1146, 1323
BmsI GCATC 2 cut(s) 298, 625
BpiI GAAGAC 3 cut(s) 62, 483, 1218
BpmI CTGGAG 2 cut(s) 126, 741
Bpu14I TTCGAA 1 cut(s) 229
BpuEI CTTGAG 1 cut(s) 440
BsaAI YACGTR 1 cut(s) 788
BsaHI GRCGYC 1 cut(s) 41
BsaJI CCNNGG 2 cut(s) 976, 1144
Bsc4I CCNNNNNNNGG 4 cut(s) 968, 1115, 1145, 1237
Bse1I ACTGG 4 cut(s) 653, 750, 919, 1274
Bse21I CCTNAGG 1 cut(s) 342
BseBI CCWGG 5 cut(s) 779, 978, 999, 1146, 1323
BseDI CCNNGG 2 cut(s) 976, 1144
BseGI GGATG 2 cut(s) 201, 1139
BseLI CCNNNNNNNGG 4 cut(s) 968, 1115, 1145, 1237
BseMII CTCAG 3 cut(s) 356, 618, 731
BseNI ACTGG 4 cut(s) 653, 750, 919, 1274
BseSI GKGCMC 1 cut(s) 859
BseXI GCAGC 6 cut(s) 4, 383, 400, 457, 460, 1006
BsgI GTGCAG 2 cut(s) 399, 1431
BshFI GGCC 4 cut(s) 876, 1029, 1219, 1241
BshNI GGYRCC 2 cut(s) 40, 1105
BsiHKAI GWGCWC 1 cut(s) 859
BsiSI CCGG 1 cut(s) 38
BsiWI CGTACG 1 cut(s) 60
BslI CCNNNNNNNGG 4 cut(s) 968, 1115, 1145, 1237
BsmAI GTCTC 1 cut(s) 736
BsmI GAATGC 3 cut(s) 929, 1159, 1395
BsnI GGCC 4 cut(s) 876, 1029, 1219, 1241
Bsp119I TTCGAA 1 cut(s) 229
Bsp1286I GDGCHC 2 cut(s) 859, 1186
Bsp143I GATC 7 cut(s) 93, 238, 280, 358, 452, 695, 1080
BspACI CCGC 5 cut(s) 24, 30, 33, 638, 659
BspANI GGCC 4 cut(s) 876, 1029, 1219, 1241
BspCNI CTCAG 3 cut(s) 355, 617, 732
BspLI GGNNCC 6 cut(s) 10, 42, 137, 440, 555, 1107
BspPI GGATC 1 cut(s) 233
BspQI GCTCTTC 1 cut(s) 113
BspT104I TTCGAA 1 cut(s) 229
BspT107I GGYRCC 2 cut(s) 40, 1105
BspTI CTTAAG 1 cut(s) 296
BsrI ACTGG 4 cut(s) 653, 750, 919, 1274
BssECI CCNNGG 2 cut(s) 976, 1144
BssMI GATC 7 cut(s) 93, 238, 280, 358, 452, 695, 1080
BssNI GRCGYC 1 cut(s) 41
Bst2UI CCWGG 5 cut(s) 779, 978, 999, 1146, 1323
Bst4CI ACNGT 3 cut(s) 335, 1253, 1305
Bst6I CTCTTC 1 cut(s) 113
BstACI GRCGYC 1 cut(s) 41
BstAFI CTTAAG 1 cut(s) 296
BstBAI YACGTR 1 cut(s) 788
BstBI TTCGAA 1 cut(s) 229
BstC8I GCNNGC 1 cut(s) 22
BstDEI CTNAG 4 cut(s) 342, 535, 604, 740
BstF5I GGATG 2 cut(s) 201, 1139
BstH2I RGCGCY 1 cut(s) 44
BstHHI GCGC 1 cut(s) 43
BstKTI GATC 7 cut(s) 96, 241, 283, 361, 455, 698, 1083
BstMAI GTCTC 1 cut(s) 736
BstMBI GATC 7 cut(s) 93, 238, 280, 358, 452, 695, 1080
BstMWI GCNNNNNNNGC 4 cut(s) 30, 422, 1003, 1409
BstNI CCWGG 5 cut(s) 779, 978, 999, 1146, 1323
BstSCI CCNGG 5 cut(s) 777, 976, 997, 1144, 1321
BstSLI GKGCMC 1 cut(s) 859
BstV1I GCAGC 6 cut(s) 4, 383, 400, 457, 460, 1006
BstV2I GAAGAC 3 cut(s) 62, 483, 1218
Bsu36I CCTNAGG 1 cut(s) 342
BsuRI GGCC 4 cut(s) 876, 1029, 1219, 1241
BtgZI GCGATG 1 cut(s) 293
BtsCI GGATG 2 cut(s) 201, 1139
Cac8I GCNNGC 1 cut(s) 22
CaiI CAGNNNCTG 1 cut(s) 914
CfoI GCGC 1 cut(s) 43
Cfr13I GGNCC 2 cut(s) 35, 553
CpoI CGGWCCG 1 cut(s) 35
CsiI ACCWGGT 2 cut(s) 777, 1321
Csp6I GTAC 2 cut(s) 61, 1106
CspI CGGWCCG 1 cut(s) 35
CviAII CATG 2 cut(s) 67, 772
CviQI GTAC 2 cut(s) 61, 1106
DdeI CTNAG 4 cut(s) 342, 535, 604, 740
DinI GGCGCC 1 cut(s) 42
DpnI GATC 7 cut(s) 95, 240, 282, 360, 454, 697, 1082
DpnII GATC 7 cut(s) 93, 238, 280, 358, 452, 695, 1080
EaeI YGGCCR 1 cut(s) 1027
Eam1104I CTCTTC 1 cut(s) 113
EarI CTCTTC 1 cut(s) 113
EciI GGCGGA 1 cut(s) 39
Eco147I AGGCCT 2 cut(s) 876, 1219
Eco24I GRGCYC 1 cut(s) 1186
Eco47I GGWCC 2 cut(s) 35, 553
Eco57I CTGAAG 2 cut(s) 74, 452
Eco81I CCTNAGG 1 cut(s) 342
EcoO109I RGGNCCY 1 cut(s) 553
EcoRI GAATTC 1 cut(s) 146
EcoRII CCWGG 5 cut(s) 777, 976, 997, 1144, 1321
EcoT38I GRGCYC 1 cut(s) 1186
EgeI GGCGCC 1 cut(s) 42
EheI GGCGCC 1 cut(s) 42
FaeI CATG 2 cut(s) 70, 775
FalI AAGNNNNNCTT 4 cut(s) 107, 139, 402, 434
FatI CATG 2 cut(s) 66, 771
FbaI TGATCA 1 cut(s) 1080
Fnu4HI GCNGC 7 cut(s) 18, 31, 372, 414, 446, 449, 995
FokI GGATG 2 cut(s) 188, 1126
FriOI GRGCYC 1 cut(s) 1186
Fsp4HI GCNGC 7 cut(s) 18, 31, 372, 414, 446, 449, 995
FspBI CTAG 5 cut(s) 195, 575, 693, 899, 1112
GlaI GCGC 1 cut(s) 42
GluI GCNGC 7 cut(s) 18, 31, 372, 414, 446, 449, 995
GsuI CTGGAG 2 cut(s) 126, 741
HaeII RGCGCY 1 cut(s) 44
HaeIII GGCC 4 cut(s) 876, 1029, 1219, 1241
HapII CCGG 1 cut(s) 38
HhaI GCGC 1 cut(s) 43
Hin1I GRCGYC 1 cut(s) 41
Hin1II CATG 2 cut(s) 70, 775
Hin6I GCGC 1 cut(s) 41
HinP1I GCGC 1 cut(s) 41
HindIII AAGCTT 1 cut(s) 464
HinfI GANTC 4 cut(s) 77, 590, 941, 1169
HpaII CCGG 1 cut(s) 38
Hpy166II GTNNAC 2 cut(s) 73, 857
Hpy188I TCNGA 6 cut(s) 700, 769, 841, 910, 1174, 1333
Hpy188III TCNNGA 9 cut(s) 133, 143, 242, 362, 377, 551, 693, 951, 1121
Hpy8I GTNNAC 2 cut(s) 73, 857
Hpy99I CGWCG 2 cut(s) 88, 131
HpyAV CCTTC 4 cut(s) 55, 866, 1017, 1196
HpyCH4III ACNGT 3 cut(s) 335, 1253, 1305
HpyCH4IV ACGT 2 cut(s) 59, 787
HpyCH4V TGCA 7 cut(s) 416, 857, 887, 929, 994, 1131, 1412
HpyF10VI GCNNNNNNNGC 4 cut(s) 30, 422, 1003, 1409
HpyF3I CTNAG 4 cut(s) 342, 535, 604, 740
HpySE526I ACGT 2 cut(s) 59, 787
Hsp92I GRCGYC 1 cut(s) 41
Hsp92II CATG 2 cut(s) 70, 775
HspAI GCGC 1 cut(s) 41
KasI GGCGCC 1 cut(s) 40
KpnI GGTACC 1 cut(s) 1109
Ksp22I TGATCA 1 cut(s) 1080
Kzo9I GATC 7 cut(s) 93, 238, 280, 358, 452, 695, 1080
LguI GCTCTTC 1 cut(s) 113
LmnI GCTCC 2 cut(s) 168, 505
Lsp1109I GCAGC 6 cut(s) 4, 383, 400, 457, 460, 1006
LweI GCATC 2 cut(s) 298, 625
MabI ACCWGGT 2 cut(s) 777, 1321
MaeI CTAG 5 cut(s) 195, 575, 693, 899, 1112
MaeII ACGT 2 cut(s) 59, 787
MaeIII GTNAC 2 cut(s) 948, 1040
MalI GATC 7 cut(s) 95, 240, 282, 360, 454, 697, 1082
MboI GATC 7 cut(s) 93, 238, 280, 358, 452, 695, 1080
MhlI GDGCHC 2 cut(s) 859, 1186
MlsI TGGCCA 1 cut(s) 1029
MluCI AATT 7 cut(s) 146, 269, 520, 585, 709, 749, 1354
MluNI TGGCCA 1 cut(s) 1029
Mly113I GGCGCC 1 cut(s) 41
MlyI GAGTC 1 cut(s) 71
MmeI TCCRAC 3 cut(s) 135, 453, 949
MnlI CCTC 6 cut(s) 20, 22, 150, 351, 415, 1231
Mox20I TGGCCA 1 cut(s) 1029
MroXI GAANNNNTTC 2 cut(s) 122, 465
MscI TGGCCA 1 cut(s) 1029
MseI TTAA 2 cut(s) 297, 1046
Msp20I TGGCCA 1 cut(s) 1029
MspA1I CMGCKG 1 cut(s) 761
MspCI CTTAAG 1 cut(s) 296
MspI CCGG 1 cut(s) 38
MspR9I CCNGG 5 cut(s) 779, 978, 999, 1146, 1323
Mva1269I GAATGC 3 cut(s) 929, 1159, 1395
MvaI CCWGG 5 cut(s) 779, 978, 999, 1146, 1323
MwoI GCNNNNNNNGC 4 cut(s) 30, 422, 1003, 1409
NarI GGCGCC 1 cut(s) 41
NdeII GATC 7 cut(s) 93, 238, 280, 358, 452, 695, 1080
NlaIII CATG 2 cut(s) 70, 775
NlaIV GGNNCC 6 cut(s) 10, 42, 137, 440, 555, 1107
NmeAIII GCCGAG 2 cut(s) 59, 133
NmuCI GTSAC 2 cut(s) 948, 1040
NspV TTCGAA 1 cut(s) 229
PceI AGGCCT 2 cut(s) 876, 1219
PciSI GCTCTTC 1 cut(s) 113
PctI GAATGC 3 cut(s) 929, 1159, 1395
PdmI GAANNNNTTC 2 cut(s) 122, 465
PfeI GAWTC 3 cut(s) 590, 941, 1169
Pfl23II CGTACG 1 cut(s) 60
PflMI CCANNNNNTGG 1 cut(s) 968
PkrI GCNGC 7 cut(s) 19, 32, 373, 415, 447, 450, 996
PleI GAGTC 1 cut(s) 71
PluTI GGCGCC 1 cut(s) 44
PpsI GAGTC 1 cut(s) 71
Ppu21I YACGTR 1 cut(s) 788
PpuMI RGGWCCY 1 cut(s) 553
Psp5II RGGWCCY 1 cut(s) 553
Psp6I CCWGG 5 cut(s) 777, 976, 997, 1144, 1321
PspGI CCWGG 5 cut(s) 777, 976, 997, 1144, 1321
PspLI CGTACG 1 cut(s) 60
PspN4I GGNNCC 6 cut(s) 10, 42, 137, 440, 555, 1107
PspPI GGNCC 2 cut(s) 35, 553
PspPPI RGGWCCY 1 cut(s) 553
PstNI CAGNNNCTG 1 cut(s) 914
PvuII CAGCTG 1 cut(s) 761
RsaI GTAC 2 cut(s) 62, 1107
RsaNI GTAC 2 cut(s) 61, 1106
Rsr2I CGGWCCG 1 cut(s) 35
RsrII CGGWCCG 1 cut(s) 35
SapI GCTCTTC 1 cut(s) 113
SaqAI TTAA 2 cut(s) 297, 1046
SatI GCNGC 7 cut(s) 18, 31, 372, 414, 446, 449, 995
Sau3AI GATC 7 cut(s) 93, 238, 280, 358, 452, 695, 1080
Sau96I GGNCC 2 cut(s) 35, 553
SchI GAGTC 1 cut(s) 71
ScrFI CCNGG 5 cut(s) 779, 978, 999, 1146, 1323
SduI GDGCHC 2 cut(s) 859, 1186
SexAI ACCWGGT 2 cut(s) 777, 1321
SfaNI GCATC 2 cut(s) 298, 625
SfoI GGCGCC 1 cut(s) 42
SfuI TTCGAA 1 cut(s) 229
SinI GGWCC 2 cut(s) 35, 553
SmlI CTYRAG 2 cut(s) 296, 419
SmoI CTYRAG 2 cut(s) 296, 419
Sse9I AATT 7 cut(s) 146, 269, 520, 585, 709, 749, 1354
SseBI AGGCCT 2 cut(s) 876, 1219
SsiI CCGC 5 cut(s) 24, 30, 33, 638, 659
SspDI GGCGCC 1 cut(s) 40
SspI AATATT 2 cut(s) 304, 430
SspMI CTAG 5 cut(s) 195, 575, 693, 899, 1112
StuI AGGCCT 2 cut(s) 876, 1219
StyD4I CCNGG 5 cut(s) 777, 976, 997, 1144, 1321
TaaI ACNGT 3 cut(s) 335, 1253, 1305
TaiI ACGT 2 cut(s) 62, 790
TaqI TCGA 1 cut(s) 229
TasI AATT 7 cut(s) 146, 269, 520, 585, 709, 749, 1354
TauI GCSGC 1 cut(s) 33
TfiI GAWTC 3 cut(s) 590, 941, 1169
Tru1I TTAA 2 cut(s) 297, 1046
Tru9I TTAA 2 cut(s) 297, 1046
TseFI GTSAC 2 cut(s) 948, 1040
TseI GCWGC 6 cut(s) 17, 371, 413, 445, 448, 994
Tsp45I GTSAC 2 cut(s) 948, 1040
TspGWI ACGGA 6 cut(s) 21, 101, 194, 237, 1328, 1359
Van91I CCANNNNNTGG 1 cut(s) 968
Vha464I CTTAAG 1 cut(s) 296
VneI GTGCAC 1 cut(s) 855
VpaK11BI GGWCC 2 cut(s) 35, 553
XapI RAATTY 4 cut(s) 146, 269, 520, 709
XbaI TCTAGA 1 cut(s) 692
XmnI GAANNNNTTC 2 cut(s) 122, 465
XspI CTAG 5 cut(s) 195, 575, 693, 899, 1112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.