pycom420g00940

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_420
OG0013239
Physical Location & Seq
Forward (+)
1251042 .. 1255027
3986 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom420g00940.1

Sequence Viewer

Length: 1452 bp
ATGGAAGGAGCTTCGACGGCTGGCGGAGGCGGTGGCCCGGCGCCGTTTCTGCTGAAGACGTACGACATGGTGGACGATTCCGCGACGGACGAGATCGTGTCGTGGAGCTCGAATAAGAAGAGCTTCATCGTCTGGAATCCGCCCGAGTTTGCTCGGGTTCTGCTCCCCACCTATTTCAAGCACAACAACTTCTCCAGCTTCATTCGTCAGCTCAATACATACGGATTTCGAAAGATTGATCCTGAGAGATGGGAATTTGCTAATGAAGACTTCATACAAGATCAAAAGCATCTTCTTAAGAATATCCACCGCAGAAAACCCATTCACAGCCACAGCAATCCTCAAGGTTCTATGGTTGATTCAGAAAGAGCAGCTCTTGATGACGAAATAGAGAAGCTTTCACATGATAAAGCCGCACTCGAGGCAAATATTTCAAGGTTCAAGCAGCAGCGATCTGAGGCAAAGCTTCAGTTGGAAGACTTAACACAACGGGTGAGTGGTATGGAACAACGGCAGAAGAATTTGGTGACATTCTTAGATAGGGCTGTTCACAACCCTAATTTTGTTGAACATCTCACTAGAAAAATTGAATCTATGGATTTCTCAGCATATCATAAGAAAAGGCGATTGCCTGACATTGACCATCTACAGCCAGTCATGGAGAATGGTTTTGTGGATAACCAAAGTAGTTCTAGATCTGAGTTTGGAAATATTTTCCACCAAGACTTCTCAAGTAAACTTAGACTTGAATTATCACCAGCTGTTTCAGACATGAATTTGGTTTCACGTAGCACGCAGAGTTCCAATGAAGATGGGGGTAGTTCAACTAGGAAAATATCTGAAGAACTTAAAGGTGCACAGATGAGAACAGAAGGTGTTTTATTTGCACCTGAAACCTTAGAACTTTCAGATACTGGAACATCTTTTGCATTCAAAATGGATTCATTGTTATCGCGGAAAGCACCAACTGTTGGAAGCCCTAGACGTCATTCCTTGGAGCCAGGTTTGCCTTCTAATGAAGAAGGTGATGGCCATTTATCCTGCCACTTAAATCTCACTTTAGCATCTACTCCGTTGCAAGTCAATAATAGTCCTTATTTAGCTACAGTACCCCAAGTAGGTCAGGATATCAACGAATCTGCAGCATCAGAGCTAAATGAAAATGGAAAAGATTCGGATTTAAGAGCCTTTACAAACAAAAATATAGCTGATGATGACAAACCCAAAACTTCCTCCCGAGAAGCCACACATAACAATCAAGTGCCCCCACCGGTTCCAGTTAGAGTAAACGATGTTTTCTGGGAACAGTTCCTAACTGAAAGGCCTGGCTGTTCAGAGAATGAAGAAGCAAGTTCAAATTATAGGGGAAATCCATTTGATGAGCAAGATGATGGAAGGTTAGACCATGGAATGTCCAGAAATGTCAAGGATGCGGAAACGCTCACTCTTTGA

Protein Analysis

484

Amino Acids

54.27

Weight (kDa)

5.46

Isoelectric Point (pI)

59.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 16 - 105 5.6e-29 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013239)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13980
fragaria_vesca FvH4_4g13230
malus_domestica MD00G1118600.v1.1 MD04G1017400.v1.1
prunus_persica Prupe.1G165500_v2.0.a1
pyrus_communis pycom04g01380 pycom420g00940
rosa_chinensis RchiOBHm_Chr4g0413481
rosa_laevigata RLG00000008216
rosa_multiflora Rmu_sc0000883.1_g000011
rosa_roxburghii Rroxscaffold_5G00357380
rosa_rugosa Rorug04G0120600
rosa_samantha Rh4AG180300 Rh4BG179600 Rh4CG191300 Rh4DG175500
rosa_wichuraiana Rw4G015010 Rw4G015070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 988
AccB1I GGYRCC 1 cut(s) 40
AccB7I CCANNNNNTGG 1 cut(s) 971
AccII CGCG 2 cut(s) 83, 955
AciI CCGC 8 cut(s) 24, 30, 81, 140, 310, 414, 955, 1433
AclWI GGATC 1 cut(s) 233
AcoI YGGCCR 1 cut(s) 1030
AcsI RAATTY 3 cut(s) 254, 520, 775
AcuI CTGAAG 3 cut(s) 74, 452, 861
AcyI GRCGYC 2 cut(s) 41, 985
AfaI GTAC 2 cut(s) 62, 1110
AfiI CCNNNNNNNGG 2 cut(s) 971, 1118
AflII CTTAAG 1 cut(s) 296
AgeI ACCGGT 1 cut(s) 1270
AgsI TTSAA 9 cut(s) 178, 435, 442, 569, 590, 749, 825, 934, 1356
AjnI CCWGG 2 cut(s) 1000, 1324
Alw21I GWGCWC 2 cut(s) 110, 859
Alw44I GTGCAC 1 cut(s) 855
AlwI GGATC 1 cut(s) 233
AlwNI CAGNNNCTG 1 cut(s) 914
Ama87I CYCGRG 4 cut(s) 143, 153, 419, 1236
AoxI GGCC 3 cut(s) 34, 1030, 1322
ApaLI GTGCAC 1 cut(s) 855
ApeKI GCWGC 4 cut(s) 371, 445, 448, 1142
ApoI RAATTY 3 cut(s) 254, 520, 775
AsiGI ACCGGT 1 cut(s) 1270
Asp700I GAANNNNTTC 2 cut(s) 122, 1171
AspLEI GCGC 1 cut(s) 43
AspS9I GGNCC 1 cut(s) 35
AsuC2I CCSGG 1 cut(s) 38
AsuHPI GGTGA 4 cut(s) 505, 538, 747, 1037
AsuII TTCGAA 1 cut(s) 229
AvaI CYCGRG 4 cut(s) 143, 153, 419, 1236
BaeGI GKGCMC 2 cut(s) 859, 1266
BalI TGGCCA 1 cut(s) 1032
BanI GGYRCC 1 cut(s) 40
BanII GRGCYC 1 cut(s) 110
BarI GAAGNNNNNNTAC 2 cut(s) 258, 290
BbsI GAAGAC 3 cut(s) 62, 273, 483
Bbv12I GWGCWC 2 cut(s) 110, 859
BbvI GCAGC 4 cut(s) 383, 457, 460, 1154
BccI CCATC 5 cut(s) 243, 651, 806, 1022, 1385
BceAI ACGGC 3 cut(s) 28, 33, 527
BciT130I CCWGG 2 cut(s) 1002, 1326
BcnI CCSGG 1 cut(s) 38
BfaI CTAG 4 cut(s) 579, 693, 828, 981
BfmI CTRYAG 3 cut(s) 647, 1104, 1140
BfoI RGCGCY 1 cut(s) 44
BfrI CTTAAG 1 cut(s) 296
BglII AGATCT 1 cut(s) 695
BisI GCNGC 5 cut(s) 372, 414, 446, 449, 1143
BlsI GCNGC 5 cut(s) 373, 415, 447, 450, 1144
Bme1390I CCNGG 3 cut(s) 38, 1002, 1326
BmeT110I CYCGRG 4 cut(s) 143, 153, 419, 1236
BmgT120I GGNCC 1 cut(s) 35
BmiI GGNNCC 3 cut(s) 42, 999, 1275
BmrFI CCNGG 3 cut(s) 38, 1002, 1326
BmsI GCATC 4 cut(s) 298, 1073, 1154, 1420
BpiI GAAGAC 3 cut(s) 62, 273, 483
BpmI CTGGAG 1 cut(s) 178
Bpu14I TTCGAA 1 cut(s) 229
BpuEI CTTGAG 2 cut(s) 327, 715
BpuMI CCSGG 1 cut(s) 38
BsaAI YACGTR 1 cut(s) 788
BsaHI GRCGYC 2 cut(s) 41, 985
BsaJI CCNNGG 2 cut(s) 993, 1405
BsaWI WCCGGW 1 cut(s) 1270
BsaXI ACNNNNNCTCC 2 cut(s) 176, 206
Bsc4I CCNNNNNNNGG 2 cut(s) 971, 1118
Bse118I RCCGGY 1 cut(s) 1270
Bse1I ACTGG 3 cut(s) 653, 919, 1277
BseBI CCWGG 2 cut(s) 1002, 1326
BseDI CCNNGG 2 cut(s) 993, 1405
BseGI GGATG 1 cut(s) 1435
BseLI CCNNNNNNNGG 2 cut(s) 971, 1118
BseMII CTCAG 4 cut(s) 234, 447, 618, 690
BseNI ACTGG 3 cut(s) 653, 919, 1277
BseSI GKGCMC 2 cut(s) 859, 1266
BseXI GCAGC 4 cut(s) 383, 457, 460, 1154
Bsh1236I CGCG 2 cut(s) 83, 955
BshFI GGCC 3 cut(s) 36, 1032, 1324
BshNI GGYRCC 1 cut(s) 40
BshTI ACCGGT 1 cut(s) 1270
BsiHKAI GWGCWC 2 cut(s) 110, 859
BsiHKCI CYCGRG 4 cut(s) 143, 153, 419, 1236
BsiSI CCGG 2 cut(s) 38, 1271
BsiWI CGTACG 1 cut(s) 60
BslI CCNNNNNNNGG 2 cut(s) 971, 1118
BsmI GAATGC 1 cut(s) 929
BsnI GGCC 3 cut(s) 36, 1032, 1324
BsoBI CYCGRG 4 cut(s) 143, 153, 419, 1236
Bsp119I TTCGAA 1 cut(s) 229
Bsp1286I GDGCHC 3 cut(s) 110, 859, 1266
Bsp143I GATC 5 cut(s) 93, 238, 280, 452, 695
Bsp19I CCATGG 1 cut(s) 1405
BspACI CCGC 8 cut(s) 24, 30, 81, 140, 310, 414, 955, 1433
BspANI GGCC 3 cut(s) 36, 1032, 1324
BspCNI CTCAG 4 cut(s) 235, 448, 617, 691
BspFNI CGCG 2 cut(s) 83, 955
BspLI GGNNCC 3 cut(s) 42, 999, 1275
BspMAI CTGCAG 1 cut(s) 1144
BspPI GGATC 1 cut(s) 233
BspQI GCTCTTC 1 cut(s) 113
BspT104I TTCGAA 1 cut(s) 229
BspT107I GGYRCC 1 cut(s) 40
BspTI CTTAAG 1 cut(s) 296
BsrFI RCCGGY 1 cut(s) 1270
BsrI ACTGG 3 cut(s) 653, 919, 1277
BssAI RCCGGY 1 cut(s) 1270
BssECI CCNNGG 2 cut(s) 993, 1405
BssMI GATC 5 cut(s) 93, 238, 280, 452, 695
BssNI GRCGYC 2 cut(s) 41, 985
BssT1I CCWWGG 2 cut(s) 993, 1405
Bst2UI CCWGG 2 cut(s) 1002, 1326
Bst4CI ACNGT 3 cut(s) 970, 1108, 1308
Bst6I CTCTTC 1 cut(s) 113
BstACI GRCGYC 2 cut(s) 41, 985
BstAFI CTTAAG 1 cut(s) 296
BstBAI YACGTR 1 cut(s) 788
BstBI TTCGAA 1 cut(s) 229
BstC8I GCNNGC 2 cut(s) 22, 794
BstDEI CTNAG 7 cut(s) 243, 456, 535, 604, 699, 740, 898
BstDSI CCRYGG 1 cut(s) 1405
BstF5I GGATG 1 cut(s) 1435
BstFNI CGCG 2 cut(s) 83, 955
BstH2I RGCGCY 1 cut(s) 44
BstHHI GCGC 1 cut(s) 43
BstKTI GATC 5 cut(s) 96, 241, 283, 455, 698
BstMBI GATC 5 cut(s) 93, 238, 280, 452, 695
BstMWI GCNNNNNNNGC 4 cut(s) 17, 49, 422, 1006
BstNI CCWGG 2 cut(s) 1002, 1326
BstSCI CCNGG 3 cut(s) 36, 1000, 1324
BstSFI CTRYAG 3 cut(s) 647, 1104, 1140
BstSLI GKGCMC 2 cut(s) 859, 1266
BstUI CGCG 2 cut(s) 83, 955
BstV1I GCAGC 4 cut(s) 383, 457, 460, 1154
BstV2I GAAGAC 3 cut(s) 62, 273, 483
BstX2I RGATCY 1 cut(s) 695
BstYI RGATCY 1 cut(s) 695
BsuRI GGCC 3 cut(s) 36, 1032, 1324
BtgI CCRYGG 1 cut(s) 1405
BtsCI GGATG 1 cut(s) 1435
Cac8I GCNNGC 2 cut(s) 22, 794
CaiI CAGNNNCTG 1 cut(s) 914
CfoI GCGC 1 cut(s) 43
Cfr10I RCCGGY 1 cut(s) 1270
Cfr13I GGNCC 1 cut(s) 35
Csp6I GTAC 2 cut(s) 61, 1109
CspAI ACCGGT 1 cut(s) 1270
CviAII CATG 5 cut(s) 67, 404, 658, 772, 1406
CviQI GTAC 2 cut(s) 61, 1109
DdeI CTNAG 7 cut(s) 243, 456, 535, 604, 699, 740, 898
DinI GGCGCC 1 cut(s) 42
DpnI GATC 5 cut(s) 95, 240, 282, 454, 697
DpnII GATC 5 cut(s) 93, 238, 280, 452, 695
EaeI YGGCCR 1 cut(s) 1030
Eam1104I CTCTTC 1 cut(s) 113
EarI CTCTTC 1 cut(s) 113
EciI GGCGGA 2 cut(s) 39, 129
Ecl136II GAGCTC 1 cut(s) 108
Eco130I CCWWGG 2 cut(s) 993, 1405
Eco147I AGGCCT 1 cut(s) 1324
Eco24I GRGCYC 1 cut(s) 110
Eco32I GATATC 1 cut(s) 1129
Eco53kI GAGCTC 1 cut(s) 108
Eco57I CTGAAG 3 cut(s) 74, 452, 861
Eco88I CYCGRG 4 cut(s) 143, 153, 419, 1236
EcoICRI GAGCTC 1 cut(s) 108
EcoRII CCWGG 2 cut(s) 1000, 1324
EcoRV GATATC 1 cut(s) 1129
EcoT14I CCWWGG 2 cut(s) 993, 1405
EcoT38I GRGCYC 1 cut(s) 110
EgeI GGCGCC 1 cut(s) 42
EheI GGCGCC 1 cut(s) 42
ErhI CCWWGG 2 cut(s) 993, 1405
FaeI CATG 5 cut(s) 70, 407, 661, 775, 1409
FalI AAGNNNNNCTT 2 cut(s) 107, 139
FatI CATG 5 cut(s) 66, 403, 657, 771, 1405
Fnu4HI GCNGC 5 cut(s) 372, 414, 446, 449, 1143
FokI GGATG 1 cut(s) 1442
FriOI GRGCYC 1 cut(s) 110
Fsp4HI GCNGC 5 cut(s) 372, 414, 446, 449, 1143
FspBI CTAG 4 cut(s) 579, 693, 828, 981
GlaI GCGC 1 cut(s) 42
GluI GCNGC 5 cut(s) 372, 414, 446, 449, 1143
GsuI CTGGAG 1 cut(s) 178
HaeII RGCGCY 1 cut(s) 44
HaeIII GGCC 3 cut(s) 36, 1032, 1324
HapII CCGG 2 cut(s) 38, 1271
HhaI GCGC 1 cut(s) 43
Hin1I GRCGYC 2 cut(s) 41, 985
Hin1II CATG 5 cut(s) 70, 407, 661, 775, 1409
Hin6I GCGC 1 cut(s) 41
HinP1I GCGC 1 cut(s) 41
HindIII AAGCTT 2 cut(s) 395, 464
HinfI GANTC 7 cut(s) 77, 136, 359, 590, 941, 1136, 1172
HpaII CCGG 2 cut(s) 38, 1271
HphI GGTGA 4 cut(s) 505, 538, 747, 1037
Hpy166II GTNNAC 5 cut(s) 73, 550, 737, 857, 1288
Hpy188I TCNGA 9 cut(s) 364, 457, 700, 769, 841, 910, 1150, 1177, 1336
Hpy188III TCNNGA 7 cut(s) 133, 242, 377, 693, 1124, 1236, 1416
Hpy8I GTNNAC 5 cut(s) 73, 550, 737, 857, 1288
Hpy99I CGWCG 2 cut(s) 19, 88
HpyAV CCTTC 4 cut(s) 866, 1016, 1020, 1389
HpyCH4III ACNGT 3 cut(s) 970, 1108, 1308
HpyCH4IV ACGT 3 cut(s) 59, 787, 985
HpyCH4V TGCA 5 cut(s) 857, 887, 929, 1078, 1142
HpyF10VI GCNNNNNNNGC 4 cut(s) 17, 49, 422, 1006
HpyF3I CTNAG 7 cut(s) 243, 456, 535, 604, 699, 740, 898
HpySE526I ACGT 3 cut(s) 59, 787, 985
Hsp92I GRCGYC 2 cut(s) 41, 985
Hsp92II CATG 5 cut(s) 70, 407, 661, 775, 1409
HspAI GCGC 1 cut(s) 41
KasI GGCGCC 1 cut(s) 40
Kzo9I GATC 5 cut(s) 93, 238, 280, 452, 695
LguI GCTCTTC 1 cut(s) 113
LmnI GCTCC 4 cut(s) 8, 105, 168, 997
Lsp1109I GCAGC 4 cut(s) 383, 457, 460, 1154
LweI GCATC 4 cut(s) 298, 1073, 1154, 1420
MaeI CTAG 4 cut(s) 579, 693, 828, 981
MaeII ACGT 3 cut(s) 59, 787, 985
MaeIII GTNAC 1 cut(s) 526
MalI GATC 5 cut(s) 95, 240, 282, 454, 697
MboI GATC 5 cut(s) 93, 238, 280, 452, 695
MflI RGATCY 1 cut(s) 695
MhlI GDGCHC 3 cut(s) 110, 859, 1266
MlsI TGGCCA 1 cut(s) 1032
MluCI AATT 7 cut(s) 254, 520, 559, 585, 749, 775, 1357
MluNI TGGCCA 1 cut(s) 1032
Mly113I GGCGCC 1 cut(s) 41
MmeI TCCRAC 2 cut(s) 453, 952
MnlI CCTC 5 cut(s) 20, 351, 415, 451, 1243
Mox20I TGGCCA 1 cut(s) 1032
MroXI GAANNNNTTC 2 cut(s) 122, 1171
MscI TGGCCA 1 cut(s) 1032
MseI TTAA 5 cut(s) 297, 482, 849, 1049, 1181
Msp20I TGGCCA 1 cut(s) 1032
MspA1I CMGCKG 1 cut(s) 761
MspCI CTTAAG 1 cut(s) 296
MspI CCGG 2 cut(s) 38, 1271
MspR9I CCNGG 3 cut(s) 38, 1002, 1326
Mva1269I GAATGC 1 cut(s) 929
MvaI CCWGG 2 cut(s) 1002, 1326
MvnI CGCG 2 cut(s) 83, 955
MwoI GCNNNNNNNGC 4 cut(s) 17, 49, 422, 1006
NarI GGCGCC 1 cut(s) 41
NciI CCSGG 1 cut(s) 38
NcoI CCATGG 1 cut(s) 1405
NdeII GATC 5 cut(s) 93, 238, 280, 452, 695
NlaIII CATG 5 cut(s) 70, 407, 661, 775, 1409
NlaIV GGNNCC 3 cut(s) 42, 999, 1275
NmuCI GTSAC 1 cut(s) 526
NspV TTCGAA 1 cut(s) 229
PaeR7I CTCGAG 1 cut(s) 419
PceI AGGCCT 1 cut(s) 1324
PciSI GCTCTTC 1 cut(s) 113
PcsI WCGNNNNNNNCGW 1 cut(s) 107
PctI GAATGC 1 cut(s) 929
PdmI GAANNNNTTC 2 cut(s) 122, 1171
PfeI GAWTC 7 cut(s) 77, 136, 359, 590, 941, 1136, 1172
Pfl23II CGTACG 1 cut(s) 60
PflMI CCANNNNNTGG 1 cut(s) 971
PinAI ACCGGT 1 cut(s) 1270
PkrI GCNGC 5 cut(s) 373, 415, 447, 450, 1144
PluTI GGCGCC 1 cut(s) 44
Ppu21I YACGTR 1 cut(s) 788
Psp124BI GAGCTC 1 cut(s) 110
Psp6I CCWGG 2 cut(s) 1000, 1324
PspGI CCWGG 2 cut(s) 1000, 1324
PspLI CGTACG 1 cut(s) 60
PspN4I GGNNCC 3 cut(s) 42, 999, 1275
PspPI GGNCC 1 cut(s) 35
PspXI VCTCGAGB 1 cut(s) 419
PstI CTGCAG 1 cut(s) 1144
PstNI CAGNNNCTG 1 cut(s) 914
PsuI RGATCY 1 cut(s) 695
PvuII CAGCTG 1 cut(s) 761
RsaI GTAC 2 cut(s) 62, 1110
RsaNI GTAC 2 cut(s) 61, 1109
SacI GAGCTC 1 cut(s) 110
SapI GCTCTTC 1 cut(s) 113
SaqAI TTAA 5 cut(s) 297, 482, 849, 1049, 1181
SatI GCNGC 5 cut(s) 372, 414, 446, 449, 1143
Sau3AI GATC 5 cut(s) 93, 238, 280, 452, 695
Sau96I GGNCC 1 cut(s) 35
ScrFI CCNGG 3 cut(s) 38, 1002, 1326
SduI GDGCHC 3 cut(s) 110, 859, 1266
SfaNI GCATC 4 cut(s) 298, 1073, 1154, 1420
SfcI CTRYAG 3 cut(s) 647, 1104, 1140
SfoI GGCGCC 1 cut(s) 42
Sfr274I CTCGAG 1 cut(s) 419
SfuI TTCGAA 1 cut(s) 229
SlaI CTCGAG 1 cut(s) 419
SmlI CTYRAG 4 cut(s) 296, 342, 419, 730
SmoI CTYRAG 4 cut(s) 296, 342, 419, 730
Sse9I AATT 7 cut(s) 254, 520, 559, 585, 749, 775, 1357
SseBI AGGCCT 1 cut(s) 1324
SsiI CCGC 8 cut(s) 24, 30, 81, 140, 310, 414, 955, 1433
SspDI GGCGCC 1 cut(s) 40
SspI AATATT 2 cut(s) 430, 712
SspMI CTAG 4 cut(s) 579, 693, 828, 981
SstI GAGCTC 1 cut(s) 110
StuI AGGCCT 1 cut(s) 1324
StyD4I CCNGG 3 cut(s) 36, 1000, 1324
StyI CCWWGG 2 cut(s) 993, 1405
TaaI ACNGT 3 cut(s) 970, 1108, 1308
TaiI ACGT 3 cut(s) 62, 790, 988
TaqI TCGA 4 cut(s) 14, 110, 229, 420
TasI AATT 7 cut(s) 254, 520, 559, 585, 749, 775, 1357
TauI GCSGC 1 cut(s) 416
TfiI GAWTC 7 cut(s) 77, 136, 359, 590, 941, 1136, 1172
Tru1I TTAA 5 cut(s) 297, 482, 849, 1049, 1181
Tru9I TTAA 5 cut(s) 297, 482, 849, 1049, 1181
TseFI GTSAC 1 cut(s) 526
TseI GCWGC 4 cut(s) 371, 445, 448, 1142
Tsp45I GTSAC 1 cut(s) 526
TspGWI ACGGA 3 cut(s) 101, 237, 1062
Van91I CCANNNNNTGG 1 cut(s) 971
Vha464I CTTAAG 1 cut(s) 296
VneI GTGCAC 1 cut(s) 855
XapI RAATTY 3 cut(s) 254, 520, 775
XbaI TCTAGA 1 cut(s) 692
XhoI CTCGAG 1 cut(s) 419
XmnI GAANNNNTTC 2 cut(s) 122, 1171
XspI CTAG 4 cut(s) 579, 693, 828, 981
ZraI GACGTC 1 cut(s) 986
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.