MD04G1017400.v1.1

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0013239
Physical Location & Seq
Reverse (-)
2047107 .. 2050740
3634 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1017400.v1.1.491

Sequence Viewer

Length: 1452 bp
ATGGAAGGAGGCTCGACTGCCGGCGGAGGCGGTGGCCCGGCGCCGTTTCTGCTGAAGACGTACAACATGGTGGACGATTCCGCGACGGACGAGATCGTGTCGTGGAGCTCGAACAAGAAGAGCTTCGTCGTCTGGAACCCGCCCGAGTTCTCCCGGGTTCTGCTCCCCACCTATTTCAAGCACAACAACTTCTCCAGCTTCATTCGTCAGCTCAATACATACGGATTTCGAAAGAGTGATCCTGAGAGATGGGAATTTGCTAATGAAGACTTCATACAAGATCAGAAGCATCTTCTTAAGAATATCCACCGCAGAAAACCCATTCACAGCCACAGCAATCCTCAAGGTTCTATGGTTGATCCAGAAAGAGCAGCTCTTGATGACGAAATAGAGAAGCTTTCACATGATAAGGACACACTCGAGGCAAGTATTTCAACGTTCAAACAGCAGCAATCTGCAGCTAAGCTTCAGTTGGAAAACTTAGCACAGCGGGTGAGTGGTATGGAACAACGGCAGAAGAATTTGCTGACATTCTTAGATGAGGCTGTTCACAACCCTAATTTTATTGAACACCTCTCTAGAAAAATTGAATCTATGGATTTCTCAGCATATAATAAGAAAAGGCGATTGCCTGACATTGAAAACCTACAACCAGTCGTGGAGAATGGTTTTGTGGATAACCAAAGTAGTTCCAGATCTGAGTTTGGAAATGTTTTCCACCAAGACTTCTCAAGTAAACTTAGACTAGAATTATCACCAGCTGTTTCAGACATGAACTTGGTTTCGCTTAGCACGCAGAGTTCCAATGAAGATGGGGGTAGTTCAACTAGGAAAATATCTGAAGAACTTAAAGGTGCACAGATGAGAACAGAAGGGCTTTTATTTGCACCTGAAACCTTAGAACTTTCAGATACCGGAACATCTTTTGCATTCAAAATGGATTCGTTGTTATTGCTGAAGGCACCAACTGTTGGAAGCCCTATACGTCATTCCTTGCAGCCAGGTTTGCCTTCTAATGAAGAAGGTGATGGTCATATATCCTGCCAGTTAAATCTCACTCTTGCATCTACTCCGTCGCAAGTCAATAATAGTCCCTATTCAGCTGCAGTACCCCAAGTAGGTCAGGATATCAACAAATCTGCAGCATCAGAGCTAAATGACAATGGCAAGGAACCGGATTTAAGAGCCTTTACAAAAAAAAATCTAGCTGATGATGACAAACCTAAAACTTCCTTCCCAGAAGCCACACATAACAATCAAGCACCTGCACTGGTTTCTGTTAGAGTAAACGATGTTTTCTGGGAACAGTTCCTAACTGAAAGACCTGGCTGTTCAGAGAATGAAGAAGCGAGTTCTAATTATAGGGGAAATCCATATGATGAGCAAGATGATGGAAGGTTCGGCCATGGAATGTCCAGAACTGTCAAGGATGCGGAAACACTCACTCTTTAA

Protein Analysis

484

Amino Acids

53.8

Weight (kDa)

5.25

Isoelectric Point (pI)

54.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 16 - 105 1.5e-28 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013239)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13980
fragaria_vesca FvH4_4g13230
malus_domestica MD00G1118600.v1.1 MD04G1017400.v1.1
prunus_persica Prupe.1G165500_v2.0.a1
pyrus_communis pycom04g01380 pycom420g00940
rosa_chinensis RchiOBHm_Chr4g0413481
rosa_laevigata RLG00000008216
rosa_multiflora Rmu_sc0000883.1_g000011
rosa_roxburghii Rroxscaffold_5G00357380
rosa_rugosa Rorug04G0120600
rosa_samantha Rh4AG180300 Rh4BG179600 Rh4CG191300 Rh4DG175500
rosa_wichuraiana Rw4G015010 Rw4G015070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1273
Acc36I ACCTGC 1 cut(s) 1273
AccB1I GGYRCC 2 cut(s) 40, 961
AccB7I CCANNNNNTGG 1 cut(s) 971
AccII CGCG 1 cut(s) 83
AciI CCGC 7 cut(s) 24, 30, 81, 140, 310, 490, 1433
AclI AACGTT 1 cut(s) 437
AclWI GGATC 2 cut(s) 233, 353
AcoI YGGCCR 1 cut(s) 1402
AcsI RAATTY 2 cut(s) 254, 520
AcuI CTGAAG 4 cut(s) 74, 452, 861, 977
AcyI GRCGYC 1 cut(s) 41
AfaI GTAC 2 cut(s) 62, 1110
AfiI CCNNNNNNNGG 2 cut(s) 971, 1118
AflII CTTAAG 1 cut(s) 296
AgsI TTSAA 8 cut(s) 178, 435, 442, 569, 590, 641, 825, 934
AjnI CCWGG 2 cut(s) 1000, 1324
Alw21I GWGCWC 2 cut(s) 110, 859
Alw44I GTGCAC 1 cut(s) 855
AlwI GGATC 2 cut(s) 233, 353
Ama87I CYCGRG 3 cut(s) 143, 153, 419
AoxI GGCC 2 cut(s) 34, 1402
ApaLI GTGCAC 1 cut(s) 855
ApeKI GCWGC 6 cut(s) 371, 448, 458, 997, 1103, 1142
ApoI RAATTY 2 cut(s) 254, 520
Asp700I GAANNNNTTC 1 cut(s) 122
AspLEI GCGC 1 cut(s) 43
AspS9I GGNCC 1 cut(s) 35
AsuC2I CCSGG 3 cut(s) 38, 154, 155
AsuHPI GGTGA 3 cut(s) 505, 747, 1037
AsuII TTCGAA 1 cut(s) 229
AvaI CYCGRG 3 cut(s) 143, 153, 419
BaeGI GKGCMC 1 cut(s) 859
BanI GGYRCC 2 cut(s) 40, 961
BanII GRGCYC 1 cut(s) 110
BarI GAAGNNNNNNTAC 2 cut(s) 258, 290
BbsI GAAGAC 2 cut(s) 62, 273
Bbv12I GWGCWC 2 cut(s) 110, 859
BbvI GCAGC 6 cut(s) 383, 460, 470, 1009, 1090, 1154
BccI CCATC 4 cut(s) 243, 806, 1022, 1385
BceAI ACGGC 2 cut(s) 28, 527
BciT130I CCWGG 2 cut(s) 1002, 1326
BcnI CCSGG 3 cut(s) 38, 154, 155
BfaI CTAG 4 cut(s) 579, 746, 828, 1205
BfmI CTRYAG 3 cut(s) 456, 1104, 1140
BfoI RGCGCY 1 cut(s) 44
BfrI CTTAAG 1 cut(s) 296
BfuAI ACCTGC 1 cut(s) 1273
BglII AGATCT 1 cut(s) 695
BisI GCNGC 6 cut(s) 372, 449, 459, 998, 1104, 1143
BlpI GCTNAGC 2 cut(s) 462, 788
BlsI GCNGC 6 cut(s) 373, 450, 460, 999, 1105, 1144
Bme1390I CCNGG 5 cut(s) 38, 154, 155, 1002, 1326
BmeT110I CYCGRG 3 cut(s) 143, 153, 419
BmgT120I GGNCC 1 cut(s) 35
BmiI GGNNCC 4 cut(s) 42, 137, 963, 1173
BmrFI CCNGG 5 cut(s) 38, 154, 155, 1002, 1326
BmsI GCATC 4 cut(s) 298, 1073, 1154, 1420
BpiI GAAGAC 2 cut(s) 62, 273
BpmI CTGGAG 1 cut(s) 178
Bpu1102I GCTNAGC 2 cut(s) 462, 788
Bpu14I TTCGAA 1 cut(s) 229
BpuEI CTTGAG 2 cut(s) 327, 715
BpuMI CCSGG 3 cut(s) 38, 154, 155
BsaHI GRCGYC 1 cut(s) 41
BsaJI CCNNGG 2 cut(s) 153, 1405
BsaWI WCCGGW 2 cut(s) 914, 1174
BsaXI ACNNNNNCTCC 2 cut(s) 176, 206
Bsc4I CCNNNNNNNGG 2 cut(s) 971, 1118
Bse118I RCCGGY 1 cut(s) 20
Bse1I ACTGG 3 cut(s) 653, 1045, 1275
BseBI CCWGG 2 cut(s) 1002, 1326
BseDI CCNNGG 2 cut(s) 153, 1405
BseGI GGATG 1 cut(s) 1435
BseLI CCNNNNNNNGG 2 cut(s) 971, 1118
BseMII CTCAG 3 cut(s) 234, 618, 690
BseNI ACTGG 3 cut(s) 653, 1045, 1275
BseSI GKGCMC 1 cut(s) 859
BseXI GCAGC 6 cut(s) 383, 460, 470, 1009, 1090, 1154
BsgI GTGCAG 1 cut(s) 1251
Bsh1236I CGCG 1 cut(s) 83
BshFI GGCC 2 cut(s) 36, 1404
BshNI GGYRCC 2 cut(s) 40, 961
BsiHKAI GWGCWC 2 cut(s) 110, 859
BsiHKCI CYCGRG 3 cut(s) 143, 153, 419
BsiSI CCGG 5 cut(s) 21, 38, 154, 915, 1175
BslFI GGGAC 1 cut(s) 1077
BslI CCNNNNNNNGG 2 cut(s) 971, 1118
BsmFI GGGAC 1 cut(s) 1077
BsmI GAATGC 1 cut(s) 929
BsnI GGCC 2 cut(s) 36, 1404
BsoBI CYCGRG 3 cut(s) 143, 153, 419
Bsp119I TTCGAA 1 cut(s) 229
Bsp1286I GDGCHC 2 cut(s) 110, 859
Bsp143I GATC 5 cut(s) 93, 238, 280, 358, 695
Bsp1720I GCTNAGC 2 cut(s) 462, 788
Bsp19I CCATGG 1 cut(s) 1405
BspACI CCGC 7 cut(s) 24, 30, 81, 140, 310, 490, 1433
BspANI GGCC 2 cut(s) 36, 1404
BspCNI CTCAG 3 cut(s) 235, 617, 691
BspFNI CGCG 1 cut(s) 83
BspLI GGNNCC 4 cut(s) 42, 137, 963, 1173
BspMAI CTGCAG 3 cut(s) 460, 1108, 1144
BspMI ACCTGC 1 cut(s) 1273
BspPI GGATC 2 cut(s) 233, 353
BspQI GCTCTTC 1 cut(s) 113
BspT104I TTCGAA 1 cut(s) 229
BspT107I GGYRCC 2 cut(s) 40, 961
BspTI CTTAAG 1 cut(s) 296
BsrFI RCCGGY 1 cut(s) 20
BsrI ACTGG 3 cut(s) 653, 1045, 1275
BssAI RCCGGY 1 cut(s) 20
BssECI CCNNGG 2 cut(s) 153, 1405
BssMI GATC 5 cut(s) 93, 238, 280, 358, 695
BssNI GRCGYC 1 cut(s) 41
BssT1I CCWWGG 1 cut(s) 1405
Bst2UI CCWGG 2 cut(s) 1002, 1326
Bst4CI ACNGT 3 cut(s) 970, 1308, 1423
Bst6I CTCTTC 1 cut(s) 113
BstACI GRCGYC 1 cut(s) 41
BstAFI CTTAAG 1 cut(s) 296
BstBI TTCGAA 1 cut(s) 229
BstC8I GCNNGC 2 cut(s) 22, 794
BstDEI CTNAG 9 cut(s) 243, 462, 481, 535, 604, 699, 740, 788, 898
BstDSI CCRYGG 1 cut(s) 1405
BstF5I GGATG 1 cut(s) 1435
BstFNI CGCG 1 cut(s) 83
BstH2I RGCGCY 1 cut(s) 44
BstHHI GCGC 1 cut(s) 43
BstKTI GATC 5 cut(s) 96, 241, 283, 361, 698
BstMBI GATC 5 cut(s) 93, 238, 280, 358, 695
BstMWI GCNNNNNNNGC 3 cut(s) 49, 793, 1006
BstNI CCWGG 2 cut(s) 1002, 1326
BstSCI CCNGG 5 cut(s) 36, 152, 153, 1000, 1324
BstSFI CTRYAG 3 cut(s) 456, 1104, 1140
BstSLI GKGCMC 1 cut(s) 859
BstUI CGCG 1 cut(s) 83
BstV1I GCAGC 6 cut(s) 383, 460, 470, 1009, 1090, 1154
BstV2I GAAGAC 2 cut(s) 62, 273
BstX2I RGATCY 1 cut(s) 695
BstYI RGATCY 1 cut(s) 695
BsuRI GGCC 2 cut(s) 36, 1404
BtgI CCRYGG 1 cut(s) 1405
BtsCI GGATG 1 cut(s) 1435
BtsIMutI CAGTG 1 cut(s) 1268
BveI ACCTGC 1 cut(s) 1273
Cac8I GCNNGC 2 cut(s) 22, 794
CfoI GCGC 1 cut(s) 43
Cfr10I RCCGGY 1 cut(s) 20
Cfr13I GGNCC 1 cut(s) 35
Cfr9I CCCGGG 1 cut(s) 153
Csp6I GTAC 2 cut(s) 61, 1109
CviAII CATG 4 cut(s) 67, 404, 772, 1406
CviQI GTAC 2 cut(s) 61, 1109
DdeI CTNAG 9 cut(s) 243, 462, 481, 535, 604, 699, 740, 788, 898
DinI GGCGCC 1 cut(s) 42
DpnI GATC 5 cut(s) 95, 240, 282, 360, 697
DpnII GATC 5 cut(s) 93, 238, 280, 358, 695
EaeI YGGCCR 1 cut(s) 1402
Eam1104I CTCTTC 1 cut(s) 113
EarI CTCTTC 1 cut(s) 113
EciI GGCGGA 1 cut(s) 39
Ecl136II GAGCTC 1 cut(s) 108
Eco130I CCWWGG 1 cut(s) 1405
Eco24I GRGCYC 1 cut(s) 110
Eco32I GATATC 1 cut(s) 1129
Eco53kI GAGCTC 1 cut(s) 108
Eco57I CTGAAG 4 cut(s) 74, 452, 861, 977
Eco88I CYCGRG 3 cut(s) 143, 153, 419
EcoICRI GAGCTC 1 cut(s) 108
EcoRII CCWGG 2 cut(s) 1000, 1324
EcoRV GATATC 1 cut(s) 1129
EcoT14I CCWWGG 1 cut(s) 1405
EcoT38I GRGCYC 1 cut(s) 110
EgeI GGCGCC 1 cut(s) 42
EheI GGCGCC 1 cut(s) 42
ErhI CCWWGG 1 cut(s) 1405
FaeI CATG 4 cut(s) 70, 407, 775, 1409
FalI AAGNNNNNCTT 2 cut(s) 107, 139
FaqI GGGAC 1 cut(s) 1077
FatI CATG 4 cut(s) 66, 403, 771, 1405
FauI CCCGC 2 cut(s) 147, 483
FauNDI CATATG 1 cut(s) 1375
Fnu4HI GCNGC 6 cut(s) 372, 449, 459, 998, 1104, 1143
FokI GGATG 1 cut(s) 1442
FriOI GRGCYC 1 cut(s) 110
Fsp4HI GCNGC 6 cut(s) 372, 449, 459, 998, 1104, 1143
FspBI CTAG 4 cut(s) 579, 746, 828, 1205
GlaI GCGC 1 cut(s) 42
GluI GCNGC 6 cut(s) 372, 449, 459, 998, 1104, 1143
GsuI CTGGAG 1 cut(s) 178
HaeII RGCGCY 1 cut(s) 44
HaeIII GGCC 2 cut(s) 36, 1404
HapII CCGG 5 cut(s) 21, 38, 154, 915, 1175
HhaI GCGC 1 cut(s) 43
Hin1I GRCGYC 1 cut(s) 41
Hin1II CATG 4 cut(s) 70, 407, 775, 1409
Hin6I GCGC 1 cut(s) 41
HinP1I GCGC 1 cut(s) 41
HindIII AAGCTT 2 cut(s) 395, 464
HinfI GANTC 3 cut(s) 77, 590, 941
HpaII CCGG 5 cut(s) 21, 38, 154, 915, 1175
HphI GGTGA 3 cut(s) 505, 747, 1037
Hpy166II GTNNAC 5 cut(s) 73, 550, 737, 857, 1288
Hpy188I TCNGA 7 cut(s) 285, 700, 769, 841, 910, 1150, 1336
Hpy188III TCNNGA 8 cut(s) 133, 242, 362, 377, 579, 693, 1124, 1416
Hpy8I GTNNAC 5 cut(s) 73, 550, 737, 857, 1288
Hpy99I CGWCG 3 cut(s) 88, 131, 1078
HpyAV CCTTC 6 cut(s) 866, 952, 1016, 1020, 1243, 1389
HpyCH4III ACNGT 3 cut(s) 970, 1308, 1423
HpyCH4IV ACGT 3 cut(s) 59, 437, 985
HpyCH4V TGCA 9 cut(s) 458, 857, 887, 929, 997, 1064, 1106, 1142, 1268
HpyF10VI GCNNNNNNNGC 3 cut(s) 49, 793, 1006
HpyF3I CTNAG 9 cut(s) 243, 462, 481, 535, 604, 699, 740, 788, 898
HpySE526I ACGT 3 cut(s) 59, 437, 985
Hsp92I GRCGYC 1 cut(s) 41
Hsp92II CATG 4 cut(s) 70, 407, 775, 1409
HspAI GCGC 1 cut(s) 41
KasI GGCGCC 1 cut(s) 40
KroI GCCGGC 1 cut(s) 20
KroNI GCCGGC 1 cut(s) 22
Kzo9I GATC 5 cut(s) 93, 238, 280, 358, 695
LguI GCTCTTC 1 cut(s) 113
LmnI GCTCC 2 cut(s) 105, 168
Lsp1109I GCAGC 6 cut(s) 383, 460, 470, 1009, 1090, 1154
LweI GCATC 4 cut(s) 298, 1073, 1154, 1420
MaeI CTAG 4 cut(s) 579, 746, 828, 1205
MaeII ACGT 3 cut(s) 59, 437, 985
MalI GATC 5 cut(s) 95, 240, 282, 360, 697
MboI GATC 5 cut(s) 93, 238, 280, 358, 695
MboII GAAGA 9 cut(s) 67, 130, 278, 284, 529, 821, 854, 1031, 1355
MflI RGATCY 1 cut(s) 695
MhlI GDGCHC 2 cut(s) 110, 859
MluCI AATT 6 cut(s) 254, 520, 559, 585, 749, 1357
Mly113I GGCGCC 1 cut(s) 41
MmeI TCCRAC 2 cut(s) 453, 952
MnlI CCTC 5 cut(s) 20, 351, 415, 535, 584
MroNI GCCGGC 1 cut(s) 20
MroXI GAANNNNTTC 1 cut(s) 122
MseI TTAA 5 cut(s) 297, 849, 1049, 1181, 1450
MspA1I CMGCKG 3 cut(s) 490, 761, 1103
MspCI CTTAAG 1 cut(s) 296
MspI CCGG 5 cut(s) 21, 38, 154, 915, 1175
MspR9I CCNGG 5 cut(s) 38, 154, 155, 1002, 1326
Mva1269I GAATGC 1 cut(s) 929
MvaI CCWGG 2 cut(s) 1002, 1326
MvnI CGCG 1 cut(s) 83
MwoI GCNNNNNNNGC 3 cut(s) 49, 793, 1006
NaeI GCCGGC 1 cut(s) 22
NarI GGCGCC 1 cut(s) 41
NciI CCSGG 3 cut(s) 38, 154, 155
NcoI CCATGG 1 cut(s) 1405
NdeI CATATG 1 cut(s) 1375
NdeII GATC 5 cut(s) 93, 238, 280, 358, 695
NgoMIV GCCGGC 1 cut(s) 20
NlaIII CATG 4 cut(s) 70, 407, 775, 1409
NlaIV GGNNCC 4 cut(s) 42, 137, 963, 1173
NspV TTCGAA 1 cut(s) 229
PaeR7I CTCGAG 1 cut(s) 419
PaqCI CACCTGC 1 cut(s) 1273
PciSI GCTCTTC 1 cut(s) 113
PcsI WCGNNNNNNNCGW 1 cut(s) 107
PctI GAATGC 1 cut(s) 929
PdiI GCCGGC 1 cut(s) 22
PdmI GAANNNNTTC 1 cut(s) 122
PfeI GAWTC 3 cut(s) 77, 590, 941
PflMI CCANNNNNTGG 1 cut(s) 971
PkrI GCNGC 6 cut(s) 373, 450, 460, 999, 1105, 1144
PluTI GGCGCC 1 cut(s) 44
Psp124BI GAGCTC 1 cut(s) 110
Psp1406I AACGTT 1 cut(s) 437
Psp6I CCWGG 2 cut(s) 1000, 1324
PspGI CCWGG 2 cut(s) 1000, 1324
PspN4I GGNNCC 4 cut(s) 42, 137, 963, 1173
PspPI GGNCC 1 cut(s) 35
PspXI VCTCGAGB 1 cut(s) 419
PstI CTGCAG 3 cut(s) 460, 1108, 1144
PsuI RGATCY 1 cut(s) 695
PvuII CAGCTG 2 cut(s) 761, 1103
RsaI GTAC 2 cut(s) 62, 1110
RsaNI GTAC 2 cut(s) 61, 1109
SacI GAGCTC 1 cut(s) 110
SapI GCTCTTC 1 cut(s) 113
SaqAI TTAA 5 cut(s) 297, 849, 1049, 1181, 1450
SatI GCNGC 6 cut(s) 372, 449, 459, 998, 1104, 1143
Sau3AI GATC 5 cut(s) 93, 238, 280, 358, 695
Sau96I GGNCC 1 cut(s) 35
ScrFI CCNGG 5 cut(s) 38, 154, 155, 1002, 1326
SduI GDGCHC 2 cut(s) 110, 859
SfaNI GCATC 4 cut(s) 298, 1073, 1154, 1420
SfcI CTRYAG 3 cut(s) 456, 1104, 1140
SfoI GGCGCC 1 cut(s) 42
Sfr274I CTCGAG 1 cut(s) 419
SfuI TTCGAA 1 cut(s) 229
SlaI CTCGAG 1 cut(s) 419
SmaI CCCGGG 1 cut(s) 155
SmlI CTYRAG 4 cut(s) 296, 342, 419, 730
SmoI CTYRAG 4 cut(s) 296, 342, 419, 730
Sse9I AATT 6 cut(s) 254, 520, 559, 585, 749, 1357
SsiI CCGC 7 cut(s) 24, 30, 81, 140, 310, 490, 1433
SspDI GGCGCC 1 cut(s) 40
SspMI CTAG 4 cut(s) 579, 746, 828, 1205
SstI GAGCTC 1 cut(s) 110
StyD4I CCNGG 5 cut(s) 36, 152, 153, 1000, 1324
StyI CCWWGG 1 cut(s) 1405
TaaI ACNGT 3 cut(s) 970, 1308, 1423
TaiI ACGT 3 cut(s) 62, 440, 988
TaqI TCGA 4 cut(s) 14, 110, 229, 420
TasI AATT 6 cut(s) 254, 520, 559, 585, 749, 1357
TfiI GAWTC 3 cut(s) 77, 590, 941
Tru1I TTAA 5 cut(s) 297, 849, 1049, 1181, 1450
Tru9I TTAA 5 cut(s) 297, 849, 1049, 1181, 1450
TscAI CASTG 1 cut(s) 1275
TseI GCWGC 6 cut(s) 371, 448, 458, 997, 1103, 1142
TspDTI ATGAA 7 cut(s) 190, 262, 279, 788, 822, 1032, 1356
TspGWI ACGGA 3 cut(s) 101, 237, 1062
TspMI CCCGGG 1 cut(s) 153
TspRI CASTG 1 cut(s) 1275
Van91I CCANNNNNTGG 1 cut(s) 971
Vha464I CTTAAG 1 cut(s) 296
VneI GTGCAC 1 cut(s) 855
XapI RAATTY 2 cut(s) 254, 520
XbaI TCTAGA 1 cut(s) 578
XhoI CTCGAG 1 cut(s) 419
XmaI CCCGGG 1 cut(s) 153
XmnI GAANNNNTTC 1 cut(s) 122
XspI CTAG 4 cut(s) 579, 746, 828, 1205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.