MD00G1125300.v1.1

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0015888
Physical Location & Seq
Forward (+)
26712745 .. 26713332
588 bp
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UTR
Exon/CDS
Intron
MD00G1125300.v1.1.491

Sequence Viewer

Length: 588 bp
ATGGAGAAGACATCGTCGTTGACTAATCCTAACACATTACCGTTCTTTGTTTTGATCGATGAAATCTTATTGCACACGATTTCTAACTGGTCAAGTAGAGTTCAGAAGGGTTTTTCAAGGTTTCAGTCTGCTTTCTTCCAATCCCAAGAGAATTGTTGTAACTCAAAGCTTTTGGAAGAGAAGAACTTGGATTCTGAGTCAAGGCGCCCGCAGCTGGTCTGCGAGAAGAGAGATGATGGGAGTTTGAGAAGAGAAGATGTGCAGAAGGTGATGGGAGACTTGGGAATTTTTTGCGGTCCAGAAGGTGAGCAACTGCCGGAGACATTCAGTTCCGATGAGCTTACAGGGCTGTTTGATGAGAAGGAGCCGAGTTTGGGGGAAGTGAAAGAAGCTTTTAACGTGTTTGATGAGAACAAAGATGGGTTTATCGATGCAAGGGACTTGCAGCGAGTTCTCTGCATATTGGGGTTAAAGGAAGGATCAAAGCTAGAAGACTGCCAGAAAATGATCAGAAGCTTTGACAAAAACGGAGATGGAAGAATAGAGTTCAATGAGTTTGTAAAAGTCATGGAGGCCAGCTTTTGCTGA

Protein Analysis

196

Amino Acids

22.26

Weight (kDa)

4.68

Isoelectric Point (pI)

53.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_1 PF00036 127 - 152 5.5e-06 EF hand domain
EF-hand_6 PF13405 127 - 156 8.7e-07 EF-hand domain
EF-hand_7 PF13499 128 - 190 1.9e-15 EF-hand domain pair
EF-hand_5 PF13202 129 - 152 7.9e-06 EF hand
EF-hand_9 PF14658 130 - 192 8.3e-06 EF-hand domain
EF-hand_8 PF13833 141 - 191 8.7e-08 EF-hand domain pair
EF-hand_1 PF00036 165 - 191 1.7e-08 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015888)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G29000 AT5G39670
fragaria_vesca FvH4_6g48240
malus_domestica MD00G1125300.v1.1 MD09G1052700.v1.1
prunus_persica Prupe.3G266000_v2.0.a1
pyrus_communis pycom17g04820
rosa_chinensis RchiOBHm_Chr2g0167751
rosa_multiflora Rmu_sc0002143.1_g000005
rosa_rugosa Rorug02G0531900
rosa_samantha Rh2AG600800 Rh2BG611400 Rh2CG581700 Rh2DG623900
rosa_wichuraiana Rw2G049870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 204
AciI CCGC 2 cut(s) 209, 294
AclWI GGATC 1 cut(s) 487
AcsI RAATTY 1 cut(s) 285
AcyI GRCGYC 1 cut(s) 205
AfiI CCNNNNNNNGG 2 cut(s) 214, 374
AflIII ACRYGT 1 cut(s) 399
AgsI TTSAA 2 cut(s) 117, 550
AluBI AGCT 7 cut(s) 169, 214, 340, 392, 487, 516, 579
AluI AGCT 7 cut(s) 169, 214, 340, 392, 487, 516, 579
Alw26I GTCTC 2 cut(s) 270, 314
AlwI GGATC 1 cut(s) 487
AoxI GGCC 1 cut(s) 573
ApeKI GCWGC 2 cut(s) 211, 445
ApoI RAATTY 1 cut(s) 285
AspLEI GCGC 1 cut(s) 207
AspS9I GGNCC 1 cut(s) 296
AsuHPI GGTGA 2 cut(s) 280, 317
AvaII GGWCC 1 cut(s) 296
BanI GGYRCC 1 cut(s) 204
BbsI GAAGAC 2 cut(s) 14, 498
BbvI GCAGC 2 cut(s) 223, 457
BccI CCATC 4 cut(s) 230, 265, 413, 527
BcgI CGANNNNNNTGC 2 cut(s) 438, 472
BclI TGATCA 1 cut(s) 507
BcoDI GTCTC 2 cut(s) 270, 314
BfaI CTAG 1 cut(s) 488
BfoI RGCGCY 1 cut(s) 208
BisI GCNGC 2 cut(s) 212, 446
BlsI GCNGC 2 cut(s) 213, 447
Bme18I GGWCC 1 cut(s) 296
BmgT120I GGNCC 1 cut(s) 296
BmiI GGNNCC 2 cut(s) 206, 366
BmsI GCATC 1 cut(s) 421
BpiI GAAGAC 2 cut(s) 14, 498
Bsa29I ATCGAT 2 cut(s) 57, 429
BsaHI GRCGYC 1 cut(s) 205
Bsc4I CCNNNNNNNGG 2 cut(s) 214, 374
Bse1I ACTGG 1 cut(s) 92
BseCI ATCGAT 2 cut(s) 57, 429
BseLI CCNNNNNNNGG 2 cut(s) 214, 374
BseMII CTCAG 1 cut(s) 186
BseNI ACTGG 1 cut(s) 92
BseXI GCAGC 2 cut(s) 223, 457
BsgI GTGCAG 1 cut(s) 281
BshFI GGCC 1 cut(s) 575
BshNI GGYRCC 1 cut(s) 204
BshVI ATCGAT 2 cut(s) 57, 429
BsiSI CCGG 1 cut(s) 317
BslFI GGGAC 1 cut(s) 452
BslI CCNNNNNNNGG 2 cut(s) 214, 374
BsmAI GTCTC 2 cut(s) 270, 314
BsmFI GGGAC 1 cut(s) 452
BsnI GGCC 1 cut(s) 575
Bsp143I GATC 3 cut(s) 54, 479, 507
BspACI CCGC 2 cut(s) 209, 294
BspANI GGCC 1 cut(s) 575
BspCNI CTCAG 1 cut(s) 187
BspDI ATCGAT 2 cut(s) 57, 429
BspLI GGNNCC 2 cut(s) 206, 366
BspPI GGATC 1 cut(s) 487
BspT107I GGYRCC 1 cut(s) 204
BsrI ACTGG 1 cut(s) 92
BssMI GATC 3 cut(s) 54, 479, 507
BssNI GRCGYC 1 cut(s) 205
Bst4CI ACNGT 1 cut(s) 42
Bst6I CTCTTC 3 cut(s) 171, 221, 244
BstACI GRCGYC 1 cut(s) 205
BstC8I GCNNGC 2 cut(s) 209, 577
BstDEI CTNAG 1 cut(s) 195
BstH2I RGCGCY 1 cut(s) 208
BstHHI GCGC 1 cut(s) 207
BstKTI GATC 3 cut(s) 57, 482, 510
BstMAI GTCTC 2 cut(s) 270, 314
BstMBI GATC 3 cut(s) 54, 479, 507
BstMWI GCNNNNNNNGC 2 cut(s) 211, 346
BstV1I GCAGC 2 cut(s) 223, 457
BstV2I GAAGAC 2 cut(s) 14, 498
Bsu15I ATCGAT 2 cut(s) 57, 429
BsuRI GGCC 1 cut(s) 575
BsuTUI ATCGAT 2 cut(s) 57, 429
Cac8I GCNNGC 2 cut(s) 209, 577
CfoI GCGC 1 cut(s) 207
Cfr13I GGNCC 1 cut(s) 296
ClaI ATCGAT 2 cut(s) 57, 429
CviAII CATG 1 cut(s) 568
DdeI CTNAG 1 cut(s) 195
DinI GGCGCC 1 cut(s) 206
DpnI GATC 3 cut(s) 56, 481, 509
DpnII GATC 3 cut(s) 54, 479, 507
Eam1104I CTCTTC 3 cut(s) 171, 221, 244
EarI CTCTTC 3 cut(s) 171, 221, 244
Eco47I GGWCC 1 cut(s) 296
EgeI GGCGCC 1 cut(s) 206
EheI GGCGCC 1 cut(s) 206
FaeI CATG 1 cut(s) 571
FaiI YATR 2 cut(s) 461, 569
FaqI GGGAC 1 cut(s) 452
FatI CATG 1 cut(s) 567
FauI CCCGC 1 cut(s) 216
FbaI TGATCA 1 cut(s) 507
Fnu4HI GCNGC 2 cut(s) 212, 446
Fsp4HI GCNGC 2 cut(s) 212, 446
FspBI CTAG 1 cut(s) 488
GlaI GCGC 1 cut(s) 206
GluI GCNGC 2 cut(s) 212, 446
HaeII RGCGCY 1 cut(s) 208
HaeIII GGCC 1 cut(s) 575
HapII CCGG 1 cut(s) 317
HhaI GCGC 1 cut(s) 207
Hin1I GRCGYC 1 cut(s) 205
Hin1II CATG 1 cut(s) 571
Hin6I GCGC 1 cut(s) 205
HinP1I GCGC 1 cut(s) 205
HincII GTYRAC 1 cut(s) 21
HindII GTYRAC 1 cut(s) 21
HindIII AAGCTT 3 cut(s) 167, 390, 514
HinfI GANTC 2 cut(s) 191, 197
HpaII CCGG 1 cut(s) 317
HphI GGTGA 2 cut(s) 280, 317
Hpy166II GTNNAC 1 cut(s) 21
Hpy188I TCNGA 4 cut(s) 105, 196, 334, 512
Hpy188III TCNNGA 1 cut(s) 299
Hpy8I GTNNAC 1 cut(s) 21
Hpy99I CGWCG 1 cut(s) 19
HpyAV CCTTC 5 cut(s) 100, 259, 296, 355, 470
HpyCH4III ACNGT 1 cut(s) 42
HpyCH4IV ACGT 1 cut(s) 399
HpyCH4V TGCA 5 cut(s) 73, 262, 434, 445, 459
HpyF10VI GCNNNNNNNGC 2 cut(s) 211, 346
HpyF3I CTNAG 1 cut(s) 195
HpySE526I ACGT 1 cut(s) 399
Hsp92I GRCGYC 1 cut(s) 205
Hsp92II CATG 1 cut(s) 571
HspAI GCGC 1 cut(s) 205
KasI GGCGCC 1 cut(s) 204
Ksp22I TGATCA 1 cut(s) 507
Kzo9I GATC 3 cut(s) 54, 479, 507
LmnI GCTCC 1 cut(s) 364
LpnPI CCDG 6 cut(s) 73, 200, 312, 330, 330, 512
Lsp1109I GCAGC 2 cut(s) 223, 457
LweI GCATC 1 cut(s) 421
MaeI CTAG 1 cut(s) 488
MaeII ACGT 1 cut(s) 399
MaeIII GTNAC 1 cut(s) 158
MalI GATC 3 cut(s) 56, 481, 509
MboI GATC 3 cut(s) 54, 479, 507
MboII GAAGA 9 cut(s) 19, 127, 188, 193, 238, 261, 266, 503, 549
MluCI AATT 2 cut(s) 151, 285
Mly113I GGCGCC 1 cut(s) 205
MlyI GAGTC 1 cut(s) 206
MnlI CCTC 1 cut(s) 565
MseI TTAA 2 cut(s) 396, 470
MspA1I CMGCKG 1 cut(s) 214
MspI CCGG 1 cut(s) 317
MwoI GCNNNNNNNGC 2 cut(s) 211, 346
NarI GGCGCC 1 cut(s) 205
NdeII GATC 3 cut(s) 54, 479, 507
NlaIII CATG 1 cut(s) 571
NlaIV GGNNCC 2 cut(s) 206, 366
NmeAIII GCCGAG 1 cut(s) 393
PfeI GAWTC 1 cut(s) 191
PflFI GACNNNGTC 1 cut(s) 13
PkrI GCNGC 2 cut(s) 213, 447
PleI GAGTC 1 cut(s) 205
PluTI GGCGCC 1 cut(s) 208
PpsI GAGTC 1 cut(s) 205
PspN4I GGNNCC 2 cut(s) 206, 366
PspPI GGNCC 1 cut(s) 296
PsyI GACNNNGTC 1 cut(s) 13
PvuII CAGCTG 1 cut(s) 214
SaqAI TTAA 2 cut(s) 396, 470
SatI GCNGC 2 cut(s) 212, 446
Sau3AI GATC 3 cut(s) 54, 479, 507
Sau96I GGNCC 1 cut(s) 296
SchI GAGTC 1 cut(s) 206
SfaNI GCATC 1 cut(s) 421
SfoI GGCGCC 1 cut(s) 206
SinI GGWCC 1 cut(s) 296
Sse9I AATT 2 cut(s) 151, 285
SsiI CCGC 2 cut(s) 209, 294
SspDI GGCGCC 1 cut(s) 204
SspMI CTAG 1 cut(s) 488
TaaI ACNGT 1 cut(s) 42
TaiI ACGT 1 cut(s) 402
TaqI TCGA 2 cut(s) 57, 429
TasI AATT 2 cut(s) 151, 285
TfiI GAWTC 1 cut(s) 191
Tru1I TTAA 2 cut(s) 396, 470
Tru9I TTAA 2 cut(s) 396, 470
TseI GCWGC 2 cut(s) 211, 445
TspDTI ATGAA 1 cut(s) 75
TspGWI ACGGA 1 cut(s) 543
Tth111I GACNNNGTC 1 cut(s) 13
VpaK11BI GGWCC 1 cut(s) 296
XapI RAATTY 1 cut(s) 285
XspI CTAG 1 cut(s) 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.