Rh2AG600800

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0015888
Physical Location & Seq
Reverse (-)
82964780 .. 82966305
1526 bp
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UTR
Exon/CDS
Intron
Rh2AG600800.1

Sequence Viewer

Length: 570 bp
ATGGAGAAGACATCATCAACATCCTCTTCACCACTTCTTGTTTTGATTGGTACAATCTTGTTACAATCGATTGCTAACTGGTTAGGTAGAATTCAGGATCTCTTTTTTAGATTTCAGTCTTTTCTCCTATCCCAGTATACCTGTGATAACTCAAAGGTTCGGGATGAGAAGAACTTGGATTCGGAGTTGAGCCAGCTGCCAAGGCTATGTGAAAAGAAGGATGATGGTAGCTTGAGCAGAGATGATGTGAACATGGTGATGGGAAACTTGGGACTTTTTTGCAGTTCAGAAGGGCTACCTGAATCATTCTGTTCTGCTGAGCTTTCAGGGCTGTTTGATGAGAACGAGCCGAGCTTGGAGGAAGTCAAACAAGCTTTTGATGTGTTTGATGAGAACAAGGATGGGTTTATCGATGCAAGAGAGTTGCAGAGAGTTCTCTGTATATTGGGCTTGAAGGAAGGTTCAAAGCTAGAAGACTGCCAGAAAATGATCGAGACATTTGATGAAAACAGAGATGGAAGGATAGAGTTCTTTGAATTTGTAAAATTTATGGAGGCTAGCTTTTGCTAA

Protein Analysis

189

Amino Acids

21.51

Weight (kDa)

4.38

Isoelectric Point (pI)

51.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 120 - 184 6.7e-14 EF-hand domain pair
EF-hand_1 PF00036 121 - 146 2.2e-06 EF hand domain
EF-hand_6 PF13405 121 - 150 5.8e-07 EF-hand domain
EF-hand_5 PF13202 123 - 146 3.9e-07 EF hand
EF-hand_8 PF13833 137 - 185 4.2e-07 EF-hand domain pair
EF-hand_1 PF00036 159 - 185 4.6e-06 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015888)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G29000 AT5G39670
fragaria_vesca FvH4_6g48240
malus_domestica MD00G1125300.v1.1 MD09G1052700.v1.1
prunus_persica Prupe.3G266000_v2.0.a1
pyrus_communis pycom17g04820
rosa_chinensis RchiOBHm_Chr2g0167751
rosa_multiflora Rmu_sc0002143.1_g000005
rosa_rugosa Rorug02G0531900
rosa_samantha Rh2AG600800 Rh2BG611400 Rh2CG581700 Rh2DG623900
rosa_wichuraiana Rw2G049870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 137
AclWI GGATC 1 cut(s) 105
AcsI RAATTY 3 cut(s) 90, 536, 545
AfaI GTAC 1 cut(s) 52
AgsI TTSAA 3 cut(s) 454, 465, 536
AluBI AGCT 7 cut(s) 196, 231, 322, 354, 374, 469, 561
AluI AGCT 7 cut(s) 196, 231, 322, 354, 374, 469, 561
Alw26I GTCTC 1 cut(s) 488
AlwI GGATC 1 cut(s) 105
ApeKI GCWGC 1 cut(s) 196
ApoI RAATTY 3 cut(s) 90, 536, 545
AsuHPI GGTGA 2 cut(s) 21, 268
AsuNHI GCTAGC 1 cut(s) 557
BbsI GAAGAC 2 cut(s) 14, 480
BbvI GCAGC 1 cut(s) 183
BccI CCATC 4 cut(s) 218, 253, 395, 509
BcoDI GTCTC 1 cut(s) 488
BfaI CTAG 2 cut(s) 470, 558
BisI GCNGC 1 cut(s) 197
BlpI GCTNAGC 1 cut(s) 318
BlsI GCNGC 1 cut(s) 198
BmrI ACTGGG 1 cut(s) 127
BmsI GCATC 1 cut(s) 403
BmtI GCTAGC 1 cut(s) 561
BmuI ACTGGG 1 cut(s) 127
BpiI GAAGAC 2 cut(s) 14, 480
Bpu1102I GCTNAGC 1 cut(s) 318
BpuEI CTTGAG 1 cut(s) 253
Bsa29I ATCGAT 2 cut(s) 68, 411
BsaJI CCNNGG 1 cut(s) 200
Bse1I ACTGG 2 cut(s) 83, 133
BseCI ATCGAT 2 cut(s) 68, 411
BseDI CCNNGG 1 cut(s) 200
BseGI GGATG 4 cut(s) 20, 169, 226, 406
BseMII CTCAG 1 cut(s) 309
BseNI ACTGG 2 cut(s) 83, 133
BseXI GCAGC 1 cut(s) 183
BshVI ATCGAT 2 cut(s) 68, 411
BslFI GGGAC 1 cut(s) 285
BsmAI GTCTC 1 cut(s) 488
BsmFI GGGAC 1 cut(s) 285
Bsp143I GATC 2 cut(s) 97, 489
Bsp1720I GCTNAGC 1 cut(s) 318
BspCNI CTCAG 1 cut(s) 310
BspDI ATCGAT 2 cut(s) 68, 411
BspOI GCTAGC 1 cut(s) 561
BspPI GGATC 1 cut(s) 105
BsrI ACTGG 2 cut(s) 83, 133
BssECI CCNNGG 1 cut(s) 200
BssMI GATC 2 cut(s) 97, 489
BssNAI GTATAC 1 cut(s) 138
BssT1I CCWWGG 1 cut(s) 200
Bst1107I GTATAC 1 cut(s) 138
Bst6I CTCTTC 1 cut(s) 31
BstC8I GCNNGC 2 cut(s) 194, 559
BstDEI CTNAG 1 cut(s) 318
BstF5I GGATG 4 cut(s) 20, 169, 226, 406
BstKTI GATC 2 cut(s) 100, 492
BstMAI GTCTC 1 cut(s) 488
BstMBI GATC 2 cut(s) 97, 489
BstMWI GCNNNNNNNGC 2 cut(s) 202, 328
BstV1I GCAGC 1 cut(s) 183
BstV2I GAAGAC 2 cut(s) 14, 480
BstX2I RGATCY 1 cut(s) 97
BstYI RGATCY 1 cut(s) 97
BstZ17I GTATAC 1 cut(s) 138
Bsu15I ATCGAT 2 cut(s) 68, 411
BsuTUI ATCGAT 2 cut(s) 68, 411
BtsCI GGATG 4 cut(s) 20, 169, 226, 406
Cac8I GCNNGC 2 cut(s) 194, 559
ClaI ATCGAT 2 cut(s) 68, 411
Csp6I GTAC 1 cut(s) 51
CviAII CATG 1 cut(s) 253
CviQI GTAC 1 cut(s) 51
DdeI CTNAG 1 cut(s) 318
DpnI GATC 2 cut(s) 99, 491
DpnII GATC 2 cut(s) 97, 489
Eam1104I CTCTTC 1 cut(s) 31
EarI CTCTTC 1 cut(s) 31
Eco130I CCWWGG 1 cut(s) 200
EcoRI GAATTC 1 cut(s) 90
EcoT14I CCWWGG 1 cut(s) 200
ErhI CCWWGG 1 cut(s) 200
FaeI CATG 1 cut(s) 256
FaiI YATR 5 cut(s) 138, 208, 254, 443, 551
FaqI GGGAC 1 cut(s) 285
FatI CATG 1 cut(s) 252
FblI GTMKAC 1 cut(s) 137
Fnu4HI GCNGC 1 cut(s) 197
FokI GGATG 4 cut(s) 7, 176, 233, 413
Fsp4HI GCNGC 1 cut(s) 197
FspBI CTAG 2 cut(s) 470, 558
GluI GCNGC 1 cut(s) 197
Hin1II CATG 1 cut(s) 256
HindIII AAGCTT 1 cut(s) 372
HinfI GANTC 2 cut(s) 179, 302
HphI GGTGA 2 cut(s) 21, 268
Hpy166II GTNNAC 2 cut(s) 138, 250
Hpy188I TCNGA 2 cut(s) 184, 289
Hpy188III TCNNGA 3 cut(s) 95, 161, 493
Hpy8I GTNNAC 2 cut(s) 138, 250
HpyAV CCTTC 5 cut(s) 211, 284, 448, 452, 513
HpyCH4V TGCA 3 cut(s) 282, 416, 427
HpyF10VI GCNNNNNNNGC 2 cut(s) 202, 328
HpyF3I CTNAG 1 cut(s) 318
Hsp92II CATG 1 cut(s) 256
Kzo9I GATC 2 cut(s) 97, 489
LpnPI CCDG 8 cut(s) 64, 80, 146, 154, 206, 312, 312, 494
Lsp1109I GCAGC 1 cut(s) 183
LweI GCATC 1 cut(s) 403
MaeI CTAG 2 cut(s) 470, 558
MaeIII GTNAC 1 cut(s) 60
MalI GATC 2 cut(s) 99, 491
MboI GATC 2 cut(s) 97, 489
MboII GAAGA 4 cut(s) 18, 19, 181, 485
MflI RGATCY 1 cut(s) 97
MluCI AATT 3 cut(s) 90, 536, 545
MnlI CCTC 3 cut(s) 34, 352, 547
MslI CAYNNNNRTG 1 cut(s) 257
MspA1I CMGCKG 1 cut(s) 196
MwoI GCNNNNNNNGC 2 cut(s) 202, 328
NdeII GATC 2 cut(s) 97, 489
NheI GCTAGC 1 cut(s) 557
NlaIII CATG 1 cut(s) 256
NmeAIII GCCGAG 1 cut(s) 375
PfeI GAWTC 2 cut(s) 179, 302
PkrI GCNGC 1 cut(s) 198
PsuI RGATCY 1 cut(s) 97
PvuII CAGCTG 1 cut(s) 196
RsaI GTAC 1 cut(s) 52
RsaNI GTAC 1 cut(s) 51
RseI CAYNNNNRTG 1 cut(s) 257
SatI GCNGC 1 cut(s) 197
Sau3AI GATC 2 cut(s) 97, 489
SfaNI GCATC 1 cut(s) 403
SmiMI CAYNNNNRTG 1 cut(s) 257
SmlI CTYRAG 1 cut(s) 232
SmoI CTYRAG 1 cut(s) 232
Sse9I AATT 3 cut(s) 90, 536, 545
SspMI CTAG 2 cut(s) 470, 558
StyI CCWWGG 1 cut(s) 200
TaqI TCGA 3 cut(s) 68, 411, 492
TasI AATT 3 cut(s) 90, 536, 545
TfiI GAWTC 2 cut(s) 179, 302
TseI GCWGC 1 cut(s) 196
TspDTI ATGAA 1 cut(s) 519
XapI RAATTY 3 cut(s) 90, 536, 545
XmiI GTMKAC 1 cut(s) 137
XspI CTAG 2 cut(s) 470, 558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.