MD09G1052700.v1.1

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0015888
Physical Location & Seq
Forward (+)
3508790 .. 3509419
630 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1052700.v1.1.491

Sequence Viewer

Length: 630 bp
ATGCTTTTCAGCCTATACTACTCTATTACAATGGAGAAGACATCGTCGTCGACTAATTCTAACTCGAACACATTACCCTTCTTCGTTTTGATCGATGCAATCTTGTTGCACGCGATTTCTAACTGCTTAAGTAGAGTTCAGAATGTTTTTTCAAGGTTTCAGTCTGTTCTCCTCCAATCCCAAGACAGTTGTTGTAACTCGACAAAGGTTTTGAATGAGAAGAAGTCGGCTTCTGAGCCAAGGCGATTGCAGATGACCTTCGAGAAGAGAGGTGATGATGGGAGTTTGAGAAGAGAAGATGTGCATATGGTGATGGGAGACTTGGGAGTTTTTTGCAGTCCAGAAGGTGAGCAACTGCCGGAGACGCTTAGTTCCGATGAGCTTTCAGGGCTGTTTGATGAGAAGGAGCCGAGCTTGGGGGAAGTGAAAGAAGCTTTTGACGTGTTTGATGAGAACAAAGATGGGTTTATCGATGCAAGGGAGTTGCAGAGAGCTCTTCGCATATTGGGGTTGAAGGAAGGATCAAAGCTAGAAGACTGCCAGAAAATGATCAGGACCTTTGACGAAAACGGAGATGGAAGGATAGAGTTTAAAGAATTTGTAAAAGTTATGGAGGCCAGCTTTTGCTGA

Protein Analysis

210

Amino Acids

23.66

Weight (kDa)

4.78

Isoelectric Point (pI)

54.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_1 PF00036 141 - 167 2.4e-07 EF hand domain
EF-hand_6 PF13405 141 - 170 4.8e-08 EF-hand domain
EF-hand_7 PF13499 142 - 204 2.1e-16 EF-hand domain pair
EF-hand_5 PF13202 143 - 164 2.2e-06 EF hand
EF-hand_9 PF14658 144 - 206 3.5e-06 EF-hand domain
EF-hand_8 PF13833 157 - 205 1.2e-10 EF-hand domain pair
EF-hand_1 PF00036 179 - 205 7.7e-08 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015888)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G29000 AT5G39670
fragaria_vesca FvH4_6g48240
malus_domestica MD00G1125300.v1.1 MD09G1052700.v1.1
prunus_persica Prupe.3G266000_v2.0.a1
pyrus_communis pycom17g04820
rosa_chinensis RchiOBHm_Chr2g0167751
rosa_multiflora Rmu_sc0002143.1_g000005
rosa_rugosa Rorug02G0531900
rosa_samantha Rh2AG600800 Rh2BG611400 Rh2CG581700 Rh2DG623900
rosa_wichuraiana Rw2G049870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 46
AccI GTMKAC 1 cut(s) 50
AccII CGCG 1 cut(s) 113
AclWI GGATC 1 cut(s) 529
AcsI RAATTY 1 cut(s) 596
AfiI CCNNNNNNNGG 1 cut(s) 416
AflII CTTAAG 1 cut(s) 127
AflIII ACRYGT 1 cut(s) 441
AgsI TTSAA 3 cut(s) 153, 214, 514
AjiI CACGTC 1 cut(s) 442
AluBI AGCT 6 cut(s) 382, 414, 434, 494, 529, 621
AluI AGCT 6 cut(s) 382, 414, 434, 494, 529, 621
Alw21I GWGCWC 1 cut(s) 496
Alw26I GTCTC 2 cut(s) 312, 356
AlwI GGATC 1 cut(s) 529
AoxI GGCC 1 cut(s) 615
ApoI RAATTY 1 cut(s) 596
ArsI GACNNNNNNTTYG 2 cut(s) 193, 225
AspS9I GGNCC 1 cut(s) 555
AsuHPI GGTGA 3 cut(s) 284, 322, 359
AvaII GGWCC 1 cut(s) 555
BanII GRGCYC 1 cut(s) 496
BbsI GAAGAC 2 cut(s) 44, 540
Bbv12I GWGCWC 1 cut(s) 496
BccI CCATC 4 cut(s) 272, 307, 455, 569
BclI TGATCA 1 cut(s) 549
BcoDI GTCTC 2 cut(s) 312, 356
BfaI CTAG 1 cut(s) 530
BfrI CTTAAG 1 cut(s) 127
Bme18I GGWCC 1 cut(s) 555
BmgBI CACGTC 1 cut(s) 442
BmgT120I GGNCC 1 cut(s) 555
BmiI GGNNCC 1 cut(s) 408
BmsI GCATC 2 cut(s) 85, 463
BpiI GAAGAC 2 cut(s) 44, 540
Bsa29I ATCGAT 2 cut(s) 93, 471
BsaJI CCNNGG 1 cut(s) 239
Bsc4I CCNNNNNNNGG 1 cut(s) 416
BseCI ATCGAT 2 cut(s) 93, 471
BseDI CCNNGG 1 cut(s) 239
BseLI CCNNNNNNNGG 1 cut(s) 416
BseMII CTCAG 1 cut(s) 225
BseRI GAGGAG 1 cut(s) 161
Bsh1236I CGCG 1 cut(s) 113
BshFI GGCC 1 cut(s) 617
BshVI ATCGAT 2 cut(s) 93, 471
BsiHKAI GWGCWC 1 cut(s) 496
BsiSI CCGG 1 cut(s) 359
BslI CCNNNNNNNGG 1 cut(s) 416
BsmAI GTCTC 2 cut(s) 312, 356
BsmBI CGTCTC 1 cut(s) 356
BsnI GGCC 1 cut(s) 617
Bsp1286I GDGCHC 1 cut(s) 496
Bsp143I GATC 3 cut(s) 90, 521, 549
BspANI GGCC 1 cut(s) 617
BspCNI CTCAG 1 cut(s) 226
BspDI ATCGAT 2 cut(s) 93, 471
BspFNI CGCG 1 cut(s) 113
BspLI GGNNCC 1 cut(s) 408
BspPI GGATC 1 cut(s) 529
BspQI GCTCTTC 1 cut(s) 501
BspTI CTTAAG 1 cut(s) 127
BssECI CCNNGG 1 cut(s) 239
BssMI GATC 3 cut(s) 90, 521, 549
BssT1I CCWWGG 1 cut(s) 239
Bst4CI ACNGT 1 cut(s) 188
Bst6I CTCTTC 3 cut(s) 260, 286, 501
BstAFI CTTAAG 1 cut(s) 127
BstC8I GCNNGC 2 cut(s) 111, 619
BstDEI CTNAG 2 cut(s) 234, 368
BstFNI CGCG 1 cut(s) 113
BstKTI GATC 3 cut(s) 93, 524, 552
BstMAI GTCTC 2 cut(s) 312, 356
BstMBI GATC 3 cut(s) 90, 521, 549
BstMWI GCNNNNNNNGC 2 cut(s) 364, 388
BstUI CGCG 1 cut(s) 113
BstV2I GAAGAC 2 cut(s) 44, 540
Bsu15I ATCGAT 2 cut(s) 93, 471
BsuRI GGCC 1 cut(s) 617
BsuTUI ATCGAT 2 cut(s) 93, 471
BtrI CACGTC 1 cut(s) 442
Cac8I GCNNGC 2 cut(s) 111, 619
Cfr13I GGNCC 1 cut(s) 555
ClaI ATCGAT 2 cut(s) 93, 471
CseI GACGC 1 cut(s) 373
DdeI CTNAG 2 cut(s) 234, 368
DpnI GATC 3 cut(s) 92, 523, 551
DpnII GATC 3 cut(s) 90, 521, 549
DraI TTTAAA 1 cut(s) 592
DrdI GACNNNNNNGTC 1 cut(s) 46
DseDI GACNNNNNNGTC 1 cut(s) 46
Eam1104I CTCTTC 3 cut(s) 260, 286, 501
EarI CTCTTC 3 cut(s) 260, 286, 501
Ecl136II GAGCTC 1 cut(s) 494
Eco130I CCWWGG 1 cut(s) 239
Eco24I GRGCYC 1 cut(s) 496
Eco47I GGWCC 1 cut(s) 555
Eco53kI GAGCTC 1 cut(s) 494
EcoICRI GAGCTC 1 cut(s) 494
EcoO109I RGGNCCY 1 cut(s) 555
EcoT14I CCWWGG 1 cut(s) 239
EcoT38I GRGCYC 1 cut(s) 496
ErhI CCWWGG 1 cut(s) 239
Esp3I CGTCTC 1 cut(s) 356
FaiI YATR 5 cut(s) 16, 306, 308, 503, 611
FauNDI CATATG 1 cut(s) 306
FbaI TGATCA 1 cut(s) 549
FblI GTMKAC 1 cut(s) 50
FriOI GRGCYC 1 cut(s) 496
FspBI CTAG 1 cut(s) 530
HaeIII GGCC 1 cut(s) 617
HapII CCGG 1 cut(s) 359
HgaI GACGC 1 cut(s) 373
HincII GTYRAC 1 cut(s) 51
HindII GTYRAC 1 cut(s) 51
HindIII AAGCTT 1 cut(s) 432
HpaII CCGG 1 cut(s) 359
HphI GGTGA 3 cut(s) 284, 322, 359
Hpy166II GTNNAC 1 cut(s) 51
Hpy188I TCNGA 3 cut(s) 141, 235, 376
Hpy188III TCNNGA 3 cut(s) 262, 341, 553
Hpy8I GTNNAC 1 cut(s) 51
Hpy99I CGWCG 2 cut(s) 49, 52
HpyAV CCTTC 7 cut(s) 88, 268, 338, 397, 508, 512, 573
HpyCH4III ACNGT 1 cut(s) 188
HpyCH4IV ACGT 1 cut(s) 441
HpyCH4V TGCA 7 cut(s) 98, 109, 250, 304, 336, 476, 487
HpyF10VI GCNNNNNNNGC 2 cut(s) 364, 388
HpyF3I CTNAG 2 cut(s) 234, 368
HpySE526I ACGT 1 cut(s) 441
Ksp22I TGATCA 1 cut(s) 549
Kzo9I GATC 3 cut(s) 90, 521, 549
LguI GCTCTTC 1 cut(s) 501
LmnI GCTCC 1 cut(s) 406
LpnPI CCDG 5 cut(s) 354, 372, 372, 538, 554
LweI GCATC 2 cut(s) 85, 463
MaeI CTAG 1 cut(s) 530
MaeII ACGT 1 cut(s) 441
MaeIII GTNAC 1 cut(s) 194
MalI GATC 3 cut(s) 92, 523, 551
MboI GATC 3 cut(s) 90, 521, 549
MboII GAAGA 8 cut(s) 49, 73, 232, 277, 303, 308, 488, 545
MhlI GDGCHC 1 cut(s) 496
MluCI AATT 2 cut(s) 55, 596
MnlI CCTC 3 cut(s) 182, 263, 607
MseI TTAA 2 cut(s) 128, 591
MspCI CTTAAG 1 cut(s) 127
MspI CCGG 1 cut(s) 359
MvnI CGCG 1 cut(s) 113
MwoI GCNNNNNNNGC 2 cut(s) 364, 388
NdeI CATATG 1 cut(s) 306
NdeII GATC 3 cut(s) 90, 521, 549
NlaIV GGNNCC 1 cut(s) 408
NmeAIII GCCGAG 1 cut(s) 435
PciSI GCTCTTC 1 cut(s) 501
PcsI WCGNNNNNNNCGW 1 cut(s) 90
PflFI GACNNNGTC 1 cut(s) 43
PpuMI RGGWCCY 1 cut(s) 555
Psp124BI GAGCTC 1 cut(s) 496
Psp5II RGGWCCY 1 cut(s) 555
PspN4I GGNNCC 1 cut(s) 408
PspPI GGNCC 1 cut(s) 555
PspPPI RGGWCCY 1 cut(s) 555
PsyI GACNNNGTC 1 cut(s) 43
SacI GAGCTC 1 cut(s) 496
SalI GTCGAC 1 cut(s) 49
SapI GCTCTTC 1 cut(s) 501
SaqAI TTAA 2 cut(s) 128, 591
Sau3AI GATC 3 cut(s) 90, 521, 549
Sau96I GGNCC 1 cut(s) 555
SduI GDGCHC 1 cut(s) 496
SfaNI GCATC 2 cut(s) 85, 463
SinI GGWCC 1 cut(s) 555
SmlI CTYRAG 1 cut(s) 127
SmoI CTYRAG 1 cut(s) 127
Sse9I AATT 2 cut(s) 55, 596
SspMI CTAG 1 cut(s) 530
SstI GAGCTC 1 cut(s) 496
StyI CCWWGG 1 cut(s) 239
TaaI ACNGT 1 cut(s) 188
TaiI ACGT 1 cut(s) 444
TaqI TCGA 6 cut(s) 50, 65, 93, 200, 261, 471
TasI AATT 2 cut(s) 55, 596
Tru1I TTAA 2 cut(s) 128, 591
Tru9I TTAA 2 cut(s) 128, 591
TspGWI ACGGA 1 cut(s) 585
Tth111I GACNNNGTC 1 cut(s) 43
Vha464I CTTAAG 1 cut(s) 127
VpaK11BI GGWCC 1 cut(s) 555
XapI RAATTY 1 cut(s) 596
XmiI GTMKAC 1 cut(s) 50
XspI CTAG 1 cut(s) 530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.