Rh2CG581700

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0015888
Physical Location & Seq
Reverse (-)
76015781 .. 76016347
567 bp
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UTR
Exon/CDS
Intron
Rh2CG581700.1

Sequence Viewer

Length: 567 bp
ATGGAGAAGACATCATCAACATCCTCTTCACCATTTCTTGTTTTGATTGGTACAATCTTGTTACAATCGATTGCTAACTGGTTAGGTAGAATTCAGGAGCTCTTTTTTAGATTTCAGTCTTTTCTCCTATCCCAGTATACCTGTGATAACTCAAAGGTTCGGGATGAGAAGAACTTGGATTCGGAGTCGAGCCAGCTGCCGCTATGTAAAAAGAAGGATGATGGTAGCTTGAGCAGAGAAGATGTGAACATGGTGATGGGAAACTTGGGAATTTTTTGCAGTTCAGAAGGGCTACCTGAATCATTCTGTTCTGCTGAGCTTTCAGGGCTGTTTGATGAGAACGAGCCGAGCTTGGAGGAAGTCAAACAAGCTTTTGATGTGTTTGATGAGAACAAGGATGGGTTTATCGATGCAAGAGAGTTGCAGAGAGTTCTCTGTATATTGGGCTTGAAGGAAGGTTCAAAGCTAGAAGACTGCCAGAAAATGATCGAGACATTTGATGAAAACAGAGATGGAAGGATAGAGTTCTTTGAATTTGTAAAATTTATGGAGGCTAGCTTTTGCTAA

Protein Analysis

188

Amino Acids

21.39

Weight (kDa)

4.43

Isoelectric Point (pI)

55.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 119 - 183 6.6e-14 EF-hand domain pair
EF-hand_1 PF00036 120 - 145 2.2e-06 EF hand domain
EF-hand_6 PF13405 120 - 149 5.7e-07 EF-hand domain
EF-hand_5 PF13202 122 - 145 3.9e-07 EF hand
EF-hand_8 PF13833 136 - 184 4e-07 EF-hand domain pair
EF-hand_1 PF00036 158 - 184 4.5e-06 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015888)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G29000 AT5G39670
fragaria_vesca FvH4_6g48240
malus_domestica MD00G1125300.v1.1 MD09G1052700.v1.1
prunus_persica Prupe.3G266000_v2.0.a1
pyrus_communis pycom17g04820
rosa_chinensis RchiOBHm_Chr2g0167751
rosa_multiflora Rmu_sc0002143.1_g000005
rosa_rugosa Rorug02G0531900
rosa_samantha Rh2AG600800 Rh2BG611400 Rh2CG581700 Rh2DG623900
rosa_wichuraiana Rw2G049870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 137
AciI CCGC 1 cut(s) 200
AcsI RAATTY 4 cut(s) 90, 270, 533, 542
AfaI GTAC 1 cut(s) 52
AgsI TTSAA 3 cut(s) 451, 462, 533
AluBI AGCT 8 cut(s) 100, 196, 228, 319, 351, 371, 466, 558
AluI AGCT 8 cut(s) 100, 196, 228, 319, 351, 371, 466, 558
Alw21I GWGCWC 1 cut(s) 102
Alw26I GTCTC 1 cut(s) 485
ApeKI GCWGC 1 cut(s) 196
ApoI RAATTY 4 cut(s) 90, 270, 533, 542
AsuHPI GGTGA 2 cut(s) 21, 265
AsuNHI GCTAGC 1 cut(s) 554
BanII GRGCYC 1 cut(s) 102
BbsI GAAGAC 2 cut(s) 14, 477
Bbv12I GWGCWC 1 cut(s) 102
BbvI GCAGC 1 cut(s) 183
BccI CCATC 4 cut(s) 215, 250, 392, 506
BcgI CGANNNNNNTGC 2 cut(s) 178, 212
BcoDI GTCTC 1 cut(s) 485
BfaI CTAG 2 cut(s) 467, 555
BisI GCNGC 2 cut(s) 197, 200
BlpI GCTNAGC 1 cut(s) 315
BlsI GCNGC 2 cut(s) 198, 201
BmrI ACTGGG 1 cut(s) 127
BmsI GCATC 1 cut(s) 400
BmtI GCTAGC 1 cut(s) 558
BmuI ACTGGG 1 cut(s) 127
BpiI GAAGAC 2 cut(s) 14, 477
Bpu1102I GCTNAGC 1 cut(s) 315
BpuEI CTTGAG 1 cut(s) 250
Bsa29I ATCGAT 2 cut(s) 68, 408
Bse1I ACTGG 2 cut(s) 83, 133
BseCI ATCGAT 2 cut(s) 68, 408
BseGI GGATG 4 cut(s) 20, 169, 223, 403
BseMII CTCAG 1 cut(s) 306
BseNI ACTGG 2 cut(s) 83, 133
BseXI GCAGC 1 cut(s) 183
BshVI ATCGAT 2 cut(s) 68, 408
BsiHKAI GWGCWC 1 cut(s) 102
BsmAI GTCTC 1 cut(s) 485
Bsp1286I GDGCHC 1 cut(s) 102
Bsp143I GATC 1 cut(s) 486
Bsp1720I GCTNAGC 1 cut(s) 315
BspACI CCGC 1 cut(s) 200
BspCNI CTCAG 1 cut(s) 307
BspDI ATCGAT 2 cut(s) 68, 408
BspOI GCTAGC 1 cut(s) 558
BsrI ACTGG 2 cut(s) 83, 133
BssMI GATC 1 cut(s) 486
BssNAI GTATAC 1 cut(s) 138
Bst1107I GTATAC 1 cut(s) 138
Bst6I CTCTTC 1 cut(s) 31
BstC8I GCNNGC 2 cut(s) 194, 556
BstDEI CTNAG 1 cut(s) 315
BstF5I GGATG 4 cut(s) 20, 169, 223, 403
BstKTI GATC 1 cut(s) 489
BstMAI GTCTC 1 cut(s) 485
BstMBI GATC 1 cut(s) 486
BstMWI GCNNNNNNNGC 1 cut(s) 325
BstV1I GCAGC 1 cut(s) 183
BstV2I GAAGAC 2 cut(s) 14, 477
BstZ17I GTATAC 1 cut(s) 138
Bsu15I ATCGAT 2 cut(s) 68, 408
BsuTUI ATCGAT 2 cut(s) 68, 408
BtsCI GGATG 4 cut(s) 20, 169, 223, 403
Cac8I GCNNGC 2 cut(s) 194, 556
ClaI ATCGAT 2 cut(s) 68, 408
Csp6I GTAC 1 cut(s) 51
CviAII CATG 1 cut(s) 250
CviQI GTAC 1 cut(s) 51
DdeI CTNAG 1 cut(s) 315
DpnI GATC 1 cut(s) 488
DpnII GATC 1 cut(s) 486
Eam1104I CTCTTC 1 cut(s) 31
EarI CTCTTC 1 cut(s) 31
Ecl136II GAGCTC 1 cut(s) 100
Eco24I GRGCYC 1 cut(s) 102
Eco53kI GAGCTC 1 cut(s) 100
EcoICRI GAGCTC 1 cut(s) 100
EcoRI GAATTC 1 cut(s) 90
EcoT38I GRGCYC 1 cut(s) 102
FaeI CATG 1 cut(s) 253
FaiI YATR 5 cut(s) 138, 205, 251, 440, 548
FatI CATG 1 cut(s) 249
FblI GTMKAC 1 cut(s) 137
Fnu4HI GCNGC 2 cut(s) 197, 200
FokI GGATG 4 cut(s) 7, 176, 230, 410
FriOI GRGCYC 1 cut(s) 102
Fsp4HI GCNGC 2 cut(s) 197, 200
FspBI CTAG 2 cut(s) 467, 555
GluI GCNGC 2 cut(s) 197, 200
Hin1II CATG 1 cut(s) 253
HindIII AAGCTT 1 cut(s) 369
HinfI GANTC 3 cut(s) 179, 185, 299
HphI GGTGA 2 cut(s) 21, 265
Hpy166II GTNNAC 2 cut(s) 138, 247
Hpy188I TCNGA 2 cut(s) 184, 286
Hpy188III TCNNGA 3 cut(s) 95, 161, 490
Hpy8I GTNNAC 2 cut(s) 138, 247
HpyAV CCTTC 5 cut(s) 208, 281, 445, 449, 510
HpyCH4V TGCA 3 cut(s) 279, 413, 424
HpyF10VI GCNNNNNNNGC 1 cut(s) 325
HpyF3I CTNAG 1 cut(s) 315
Hsp92II CATG 1 cut(s) 253
Kzo9I GATC 1 cut(s) 486
LmnI GCTCC 1 cut(s) 97
LpnPI CCDG 8 cut(s) 64, 80, 146, 154, 206, 309, 309, 491
Lsp1109I GCAGC 1 cut(s) 183
LweI GCATC 1 cut(s) 400
MaeI CTAG 2 cut(s) 467, 555
MaeIII GTNAC 1 cut(s) 60
MalI GATC 1 cut(s) 488
MboI GATC 1 cut(s) 486
MboII GAAGA 5 cut(s) 18, 19, 181, 251, 482
MhlI GDGCHC 1 cut(s) 102
MluCI AATT 4 cut(s) 90, 270, 533, 542
MlyI GAGTC 1 cut(s) 194
MnlI CCTC 3 cut(s) 34, 349, 544
MslI CAYNNNNRTG 1 cut(s) 254
MspA1I CMGCKG 1 cut(s) 196
MwoI GCNNNNNNNGC 1 cut(s) 325
NdeII GATC 1 cut(s) 486
NheI GCTAGC 1 cut(s) 554
NlaIII CATG 1 cut(s) 253
NmeAIII GCCGAG 1 cut(s) 372
PfeI GAWTC 2 cut(s) 179, 299
PkrI GCNGC 2 cut(s) 198, 201
PleI GAGTC 1 cut(s) 193
PpsI GAGTC 1 cut(s) 193
Psp124BI GAGCTC 1 cut(s) 102
PvuII CAGCTG 1 cut(s) 196
RsaI GTAC 1 cut(s) 52
RsaNI GTAC 1 cut(s) 51
RseI CAYNNNNRTG 1 cut(s) 254
SacI GAGCTC 1 cut(s) 102
SatI GCNGC 2 cut(s) 197, 200
Sau3AI GATC 1 cut(s) 486
SchI GAGTC 1 cut(s) 194
SduI GDGCHC 1 cut(s) 102
SfaNI GCATC 1 cut(s) 400
SmiMI CAYNNNNRTG 1 cut(s) 254
SmlI CTYRAG 1 cut(s) 229
SmoI CTYRAG 1 cut(s) 229
Sse9I AATT 4 cut(s) 90, 270, 533, 542
SsiI CCGC 1 cut(s) 200
SspMI CTAG 2 cut(s) 467, 555
SstI GAGCTC 1 cut(s) 102
TaqI TCGA 4 cut(s) 68, 188, 408, 489
TasI AATT 4 cut(s) 90, 270, 533, 542
TauI GCSGC 1 cut(s) 202
TfiI GAWTC 2 cut(s) 179, 299
TseI GCWGC 1 cut(s) 196
TspDTI ATGAA 1 cut(s) 516
XapI RAATTY 4 cut(s) 90, 270, 533, 542
XmiI GTMKAC 1 cut(s) 137
XspI CTAG 2 cut(s) 467, 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.