MD00G1147000.v1.1

BEST Arabidopsis thaliana protein match is hydroxyproline-rich glycoprotein family protein (TAIR AT5G52430.1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0012074
Physical Location & Seq
Forward (+)
32160297 .. 32163741
3445 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1147000.v1.1.491

Sequence Viewer

Length: 1431 bp
ATGGGGTCCGAGCAGCACAGATTCCCTCAGCAGGAGCTGAGAAAGAGATGGGGAGGATGTTGGGGTGCATTTTCTTGCTTTGCCTCACAGAAAGGAGGAAAGCGCATAGTGCCTGCAACTCGCATTCCTGAAGGCAATGCATCAGCAAATCAGCCAAATGGACCCCAAACAGTTGGGCTGACCAACCAAACGACGTCACTGGCTCCTTCTCTTCTAGCTCCGCCATCTTCTCCAGCGTCATTTACAAATTCGGCCCTTCCTTCAACAGCGCAGTCTCCTAGTTGTTTCTTGTCTGCCAACTCACCTGGAGGTCCTTCATCCACCATGTATGCCACCGGGCCATATGCCAACGAAACACAACTTGTCTCTCCTCCTGTTTTCTCAACCTTCACAACTGAGCCGTCCACTGCTCCTCTCACTCCCCCACCAGAGTTGGCTCACCTAACTACTCCATCTTCTCCAGATGTGCCATTTGCTCGTTTCCTCTCATCTTCTGCAGACATTAAAAGTGCTGAGAAGGCCAATTACATTGCTGCAAATGATCTTCATTCAACATATTCACTCTATCCTGGAAGTCCTGCTAGTAGTCTCAGATCACCAATTTCAAGGGCATCAAACGATTGTTCATCATCATCTTTTCCTGAGCATGATTTCCCCCCACATTGGGATCCGTCAGCTTCTCCCCAAAATGGTACATATTCAAGGAGGGGTTCTGCCAGGATGTTTGGGCATGACACAGCTGGAGCTTCTGTGGCATGTCAAGACTCAAATTTCTTTTGTCCTGCTACATTTGCACAATTCTATCTGGATAATCCACCATTTCCTCATGCTGTTGGAAGGTTAAGCGTATCCAAGGATTCAGATGTTTACTCTACTGGTGGGAATGGAAGTCAGAACAGACACAATAGAAGTCCCAAGCAAGATGTGGAAGAAATAGAAGCGTATAGAGCATCCTTTGGGTTCAGTGCTGATGAAATTATCACCACAACACAATATGTGGAGATTTCTGATGTAATGGAGGACTCGTTTACCATGACACCTTTTCCCTCTCATAAATTACCTACAGAAGAAAGTATGGAACCGCCATCTGTCATTGAAGGACTAAAGGCACATAAGACAAAAACAAACTTGCAGAGTCAGGAGAGTCATAAATCTGAATCAGATCTTGATGAGGGTGGACGTTGTGACCTGCTCATGTCGTGTACTGGATCCGAAGATCACAAATCATGGAGGCAGTCTGGTGATGTCTCTCGATCAAGTACGCCTGGAATCCGCATCGTGGCCAATGAAGAGGACATTTTTTCAAAGATGGGGTCTTCTAAATTGAGCAGAAAGTATCAGATGGGTCAATCTAGTTCAGATGCAGAAATTGATTATAGAAGGGGAAGAAGCTTACGAGAGAGAAAAGGAGAATTTGCGTGGAATGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

477

Amino Acids

51.46

Weight (kDa)

5.78

Isoelectric Point (pI)

77.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012074)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G76660 AT1G76660
fragaria_vesca FvH4_5g26800 FvH4_5g26800 FvH4_5g26800
malus_domestica MD00G1147000.v1.1 MD16G1282800.v1.1
prunus_persica Prupe.5G000400_v2.0.a1
pyrus_communis pycom16g26420
rosa_chinensis RchiOBHm_Chr7g0217711
rosa_laevigata RLG00000002445
rosa_multiflora Rmu_sc0000202.1_g000008
rosa_roxburghii Rroxscaffold_3G00241770
rosa_rugosa Rorug07G0170700
rosa_samantha Rh7AG311200 Rh7BG303100 Rh7CG330200 Rh7DG311000
rosa_wichuraiana Rw7G026420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 197
Acc36I ACCTGC 1 cut(s) 1197
AciI CCGC 3 cut(s) 221, 1082, 1273
AclWI GGATC 4 cut(s) 662, 675, 1203, 1216
AcoI YGGCCR 1 cut(s) 1281
AcsI RAATTY 3 cut(s) 247, 769, 1412
AcuI CTGAAG 1 cut(s) 150
AcyI GRCGYC 1 cut(s) 194
AfaI GTAC 3 cut(s) 694, 1204, 1261
AfiI CCNNNNNNNGG 5 cut(s) 31, 663, 664, 689, 1279
AgsI TTSAA 6 cut(s) 264, 552, 606, 702, 1097, 1305
AjnI CCWGG 4 cut(s) 304, 568, 716, 1264
AluBI AGCT 6 cut(s) 37, 218, 677, 740, 746, 1392
AluI AGCT 6 cut(s) 37, 218, 677, 740, 746, 1392
Alw26I GTCTC 4 cut(s) 279, 370, 593, 1252
AlwI GGATC 4 cut(s) 662, 675, 1203, 1216
AlwNI CAGNNNCTG 1 cut(s) 37
AoxI GGCC 4 cut(s) 252, 338, 519, 1281
ApeKI GCWGC 2 cut(s) 13, 533
ApoI RAATTY 3 cut(s) 247, 769, 1412
ArsI GACNNNNNNTTYG 2 cut(s) 1298, 1330
AspLEI GCGC 2 cut(s) 105, 271
AspS9I GGNCC 5 cut(s) 6, 161, 253, 311, 338
AsuC2I CCSGG 1 cut(s) 337
AsuHPI GGTGA 5 cut(s) 294, 431, 588, 973, 1253
AvaII GGWCC 3 cut(s) 6, 161, 311
BalI TGGCCA 1 cut(s) 1283
BamHI GGATCC 2 cut(s) 667, 1208
BarI GAAGNNNNNNTAC 2 cut(s) 509, 541
BbsI GAAGAC 1 cut(s) 1308
BbvCI CCTCAGC 1 cut(s) 27
BbvI GCAGC 2 cut(s) 25, 520
BccI CCATC 6 cut(s) 42, 232, 460, 1093, 1303, 1336
BceAI ACGGC 1 cut(s) 385
BciT130I CCWGG 4 cut(s) 306, 570, 718, 1266
BciVI GTATCC 1 cut(s) 859
BcnI CCSGG 1 cut(s) 337
BcoDI GTCTC 4 cut(s) 279, 370, 593, 1252
BfaI CTAG 4 cut(s) 215, 279, 582, 1353
BfmI CTRYAG 2 cut(s) 495, 1062
BfuAI ACCTGC 1 cut(s) 1197
BfuI GTATCC 1 cut(s) 859
BglII AGATCT 1 cut(s) 1162
BisI GCNGC 2 cut(s) 14, 534
BlsI GCNGC 2 cut(s) 15, 535
Bme1390I CCNGG 5 cut(s) 306, 337, 570, 718, 1266
Bme18I GGWCC 3 cut(s) 6, 161, 311
BmgT120I GGNCC 5 cut(s) 6, 161, 253, 311, 338
BmiI GGNNCC 6 cut(s) 7, 163, 204, 669, 1080, 1210
BmrFI CCNGG 5 cut(s) 306, 337, 570, 718, 1266
BmsI GCATC 5 cut(s) 149, 620, 959, 1284, 1351
BpiI GAAGAC 1 cut(s) 1308
BpmI CTGGAG 4 cut(s) 216, 327, 444, 762
Bpu10I CCTNAGC 2 cut(s) 27, 642
BpuMI CCSGG 1 cut(s) 337
BsaHI GRCGYC 1 cut(s) 194
BsaJI CCNNGG 1 cut(s) 852
BsaXI ACNNNNNCTCC 4 cut(s) 735, 765, 1010, 1040
Bsc4I CCNNNNNNNGG 5 cut(s) 31, 663, 664, 689, 1279
Bse1I ACTGG 3 cut(s) 204, 880, 1210
Bse3DI GCAATG 2 cut(s) 142, 528
BseBI CCWGG 4 cut(s) 306, 570, 718, 1266
BseDI CCNNGG 1 cut(s) 852
BseGI GGATG 4 cut(s) 62, 317, 726, 950
BseLI CCNNNNNNNGG 5 cut(s) 31, 663, 664, 689, 1279
BseMI GCAATG 2 cut(s) 142, 528
BseMII CTCAG 6 cut(s) 29, 41, 387, 504, 604, 633
BseNI ACTGG 3 cut(s) 204, 880, 1210
BseRI GAGGAG 2 cut(s) 360, 402
BseXI GCAGC 2 cut(s) 25, 520
BshFI GGCC 4 cut(s) 254, 340, 521, 1283
BsiSI CCGG 1 cut(s) 336
BslFI GGGAC 1 cut(s) 897
BslI CCNNNNNNNGG 5 cut(s) 31, 663, 664, 689, 1279
BsmAI GTCTC 4 cut(s) 279, 370, 593, 1252
BsmFI GGGAC 1 cut(s) 897
BsmI GAATGC 1 cut(s) 123
BsnI GGCC 4 cut(s) 254, 340, 521, 1283
Bsp143I GATC 7 cut(s) 541, 593, 667, 1162, 1208, 1216, 1253
BspACI CCGC 3 cut(s) 221, 1082, 1273
BspANI GGCC 4 cut(s) 254, 340, 521, 1283
BspCNI CTCAG 6 cut(s) 30, 40, 388, 505, 603, 634
BspLI GGNNCC 6 cut(s) 7, 163, 204, 669, 1080, 1210
BspMAI CTGCAG 1 cut(s) 499
BspMI ACCTGC 1 cut(s) 1197
BspPI GGATC 4 cut(s) 662, 675, 1203, 1216
BsrDI GCAATG 2 cut(s) 142, 528
BsrI ACTGG 3 cut(s) 204, 880, 1210
BssECI CCNNGG 1 cut(s) 852
BssMI GATC 7 cut(s) 541, 593, 667, 1162, 1208, 1216, 1253
BssNI GRCGYC 1 cut(s) 194
BssT1I CCWWGG 1 cut(s) 852
Bst2UI CCWGG 4 cut(s) 306, 570, 718, 1266
Bst4CI ACNGT 1 cut(s) 172
Bst6I CTCTTC 2 cut(s) 216, 1284
BstACI GRCGYC 1 cut(s) 194
BstC8I GCNNGC 1 cut(s) 114
BstDEI CTNAG 6 cut(s) 27, 38, 396, 513, 590, 642
BstF5I GGATG 4 cut(s) 62, 317, 726, 950
BstHHI GCGC 2 cut(s) 105, 271
BstKTI GATC 7 cut(s) 544, 596, 670, 1165, 1211, 1219, 1256
BstMAI GTCTC 4 cut(s) 279, 370, 593, 1252
BstMBI GATC 7 cut(s) 541, 593, 667, 1162, 1208, 1216, 1253
BstMWI GCNNNNNNNGC 5 cut(s) 109, 518, 752, 791, 947
BstNI CCWGG 4 cut(s) 306, 570, 718, 1266
BstNSI RCATGY 1 cut(s) 759
BstSCI CCNGG 5 cut(s) 304, 335, 568, 716, 1264
BstSFI CTRYAG 2 cut(s) 495, 1062
BstV1I GCAGC 2 cut(s) 25, 520
BstV2I GAAGAC 1 cut(s) 1308
BstX2I RGATCY 3 cut(s) 667, 1162, 1208
BstXI CCANNNNNNTGG 1 cut(s) 173
BstYI RGATCY 3 cut(s) 667, 1162, 1208
BsuI GTATCC 1 cut(s) 859
BsuRI GGCC 4 cut(s) 254, 340, 521, 1283
BtsCI GGATG 4 cut(s) 62, 317, 726, 950
BtsI GCAGTG 1 cut(s) 405
BtsIMutI CAGTG 3 cut(s) 197, 405, 970
BveI ACCTGC 1 cut(s) 1197
Cac8I GCNNGC 1 cut(s) 114
CaiI CAGNNNCTG 1 cut(s) 37
CfoI GCGC 2 cut(s) 105, 271
Cfr13I GGNCC 5 cut(s) 6, 161, 253, 311, 338
CseI GACGC 1 cut(s) 225
Csp6I GTAC 3 cut(s) 693, 1203, 1260
CspCI CAANNNNNGTGG 2 cut(s) 414, 449
CviAII CATG 8 cut(s) 325, 647, 731, 756, 827, 1033, 1195, 1227
CviQI GTAC 3 cut(s) 693, 1203, 1260
DdeI CTNAG 6 cut(s) 27, 38, 396, 513, 590, 642
DpnI GATC 7 cut(s) 543, 595, 669, 1164, 1210, 1218, 1255
DpnII GATC 7 cut(s) 541, 593, 667, 1162, 1208, 1216, 1253
EaeI YGGCCR 1 cut(s) 1281
Eam1104I CTCTTC 2 cut(s) 216, 1284
EarI CTCTTC 2 cut(s) 216, 1284
EciI GGCGGA 1 cut(s) 210
Eco130I CCWWGG 1 cut(s) 852
Eco47I GGWCC 3 cut(s) 6, 161, 311
Eco57I CTGAAG 1 cut(s) 150
EcoO109I RGGNCCY 1 cut(s) 311
EcoRII CCWGG 4 cut(s) 304, 568, 716, 1264
EcoT14I CCWWGG 1 cut(s) 852
EcoT22I ATGCAT 1 cut(s) 142
ErhI CCWWGG 1 cut(s) 852
FaeI CATG 8 cut(s) 328, 650, 734, 759, 830, 1036, 1198, 1230
FaqI GGGAC 1 cut(s) 897
FatI CATG 8 cut(s) 324, 646, 730, 755, 826, 1032, 1194, 1226
FauNDI CATATG 1 cut(s) 343
Fnu4HI GCNGC 2 cut(s) 14, 534
FokI GGATG 4 cut(s) 69, 304, 733, 937
Fsp4HI GCNGC 2 cut(s) 14, 534
FspBI CTAG 4 cut(s) 215, 279, 582, 1353
GlaI GCGC 2 cut(s) 104, 270
GluI GCNGC 2 cut(s) 14, 534
GsuI CTGGAG 4 cut(s) 216, 327, 444, 762
HaeIII GGCC 4 cut(s) 254, 340, 521, 1283
HapII CCGG 1 cut(s) 336
HgaI GACGC 1 cut(s) 225
HhaI GCGC 2 cut(s) 105, 271
Hin1I GRCGYC 1 cut(s) 194
Hin1II CATG 8 cut(s) 328, 650, 734, 759, 830, 1036, 1198, 1230
Hin6I GCGC 2 cut(s) 103, 269
HinP1I GCGC 2 cut(s) 103, 269
HindIII AAGCTT 1 cut(s) 1390
HinfI GANTC 8 cut(s) 21, 764, 857, 1022, 1135, 1144, 1157, 1269
HpaII CCGG 1 cut(s) 336
HphI GGTGA 5 cut(s) 294, 431, 588, 973, 1253
Hpy166II GTNNAC 5 cut(s) 405, 868, 1029, 1178, 1203
Hpy188III TCNNGA 8 cut(s) 128, 461, 641, 761, 806, 1139, 1166, 1251
Hpy8I GTNNAC 5 cut(s) 405, 868, 1029, 1178, 1203
Hpy99I CGWCG 1 cut(s) 196
HpyCH4III ACNGT 1 cut(s) 172
HpyCH4IV ACGT 2 cut(s) 194, 1180
HpyCH4V TGCA 8 cut(s) 68, 116, 140, 497, 536, 794, 1132, 1364
HpyF10VI GCNNNNNNNGC 5 cut(s) 109, 518, 752, 791, 947
HpyF3I CTNAG 6 cut(s) 27, 38, 396, 513, 590, 642
HpySE526I ACGT 2 cut(s) 194, 1180
Hsp92I GRCGYC 1 cut(s) 194
Hsp92II CATG 8 cut(s) 328, 650, 734, 759, 830, 1036, 1198, 1230
HspAI GCGC 2 cut(s) 103, 269
Kzo9I GATC 7 cut(s) 541, 593, 667, 1162, 1208, 1216, 1253
LmnI GCTCC 5 cut(s) 34, 208, 223, 415, 743
Lsp1109I GCAGC 2 cut(s) 25, 520
LweI GCATC 5 cut(s) 149, 620, 959, 1284, 1351
MaeI CTAG 4 cut(s) 215, 279, 582, 1353
MaeII ACGT 2 cut(s) 194, 1180
MaeIII GTNAC 2 cut(s) 195, 1184
MalI GATC 7 cut(s) 543, 595, 669, 1164, 1210, 1218, 1255
MboI GATC 7 cut(s) 541, 593, 667, 1162, 1208, 1216, 1253
MflI RGATCY 3 cut(s) 667, 1162, 1208
MlsI TGGCCA 1 cut(s) 1283
MluNI TGGCCA 1 cut(s) 1283
MlyI GAGTC 4 cut(s) 758, 1016, 1144, 1153
MmeI TCCRAC 1 cut(s) 814
Mox20I TGGCCA 1 cut(s) 1283
Mph1103I ATGCAT 1 cut(s) 142
MscI TGGCCA 1 cut(s) 1283
MseI TTAA 2 cut(s) 504, 842
Msp20I TGGCCA 1 cut(s) 1283
MspA1I CMGCKG 1 cut(s) 740
MspI CCGG 1 cut(s) 336
MspR9I CCNGG 5 cut(s) 306, 337, 570, 718, 1266
Mva1269I GAATGC 1 cut(s) 123
MvaI CCWGG 4 cut(s) 306, 570, 718, 1266
MwoI GCNNNNNNNGC 5 cut(s) 109, 518, 752, 791, 947
NciI CCSGG 1 cut(s) 337
NdeI CATATG 1 cut(s) 343
NdeII GATC 7 cut(s) 541, 593, 667, 1162, 1208, 1216, 1253
NlaIII CATG 8 cut(s) 328, 650, 734, 759, 830, 1036, 1198, 1230
NlaIV GGNNCC 6 cut(s) 7, 163, 204, 669, 1080, 1210
NmuCI GTSAC 2 cut(s) 195, 1184
NsiI ATGCAT 1 cut(s) 142
NspI RCATGY 1 cut(s) 759
PctI GAATGC 1 cut(s) 123
PfeI GAWTC 4 cut(s) 21, 857, 1157, 1269
PfoI TCCNGGA 1 cut(s) 568
PkrI GCNGC 2 cut(s) 15, 535
PleI GAGTC 4 cut(s) 758, 1016, 1143, 1152
PpsI GAGTC 4 cut(s) 758, 1016, 1143, 1152
PpuMI RGGWCCY 1 cut(s) 311
Psp5II RGGWCCY 1 cut(s) 311
Psp6I CCWGG 4 cut(s) 304, 568, 716, 1264
PspGI CCWGG 4 cut(s) 304, 568, 716, 1264
PspN4I GGNNCC 6 cut(s) 7, 163, 204, 669, 1080, 1210
PspPI GGNCC 5 cut(s) 6, 161, 253, 311, 338
PspPPI RGGWCCY 1 cut(s) 311
PstI CTGCAG 1 cut(s) 499
PstNI CAGNNNCTG 1 cut(s) 37
PsuI RGATCY 3 cut(s) 667, 1162, 1208
PvuII CAGCTG 1 cut(s) 740
RsaI GTAC 3 cut(s) 694, 1204, 1261
RsaNI GTAC 3 cut(s) 693, 1203, 1260
SaqAI TTAA 2 cut(s) 504, 842
SatI GCNGC 2 cut(s) 14, 534
Sau3AI GATC 7 cut(s) 541, 593, 667, 1162, 1208, 1216, 1253
Sau96I GGNCC 5 cut(s) 6, 161, 253, 311, 338
SchI GAGTC 4 cut(s) 758, 1016, 1144, 1153
ScrFI CCNGG 5 cut(s) 306, 337, 570, 718, 1266
SfaNI GCATC 5 cut(s) 149, 620, 959, 1284, 1351
SfcI CTRYAG 2 cut(s) 495, 1062
SinI GGWCC 3 cut(s) 6, 161, 311
SsiI CCGC 3 cut(s) 221, 1082, 1273
SspMI CTAG 4 cut(s) 215, 279, 582, 1353
StyD4I CCNGG 5 cut(s) 304, 335, 568, 716, 1264
StyI CCWWGG 1 cut(s) 852
TaaI ACNGT 1 cut(s) 172
TaiI ACGT 2 cut(s) 197, 1183
TaqI TCGA 1 cut(s) 1252
TatI WGTACW 1 cut(s) 1202
TfiI GAWTC 4 cut(s) 21, 857, 1157, 1269
Tru1I TTAA 2 cut(s) 504, 842
Tru9I TTAA 2 cut(s) 504, 842
TscAI CASTG 3 cut(s) 204, 412, 970
TseFI GTSAC 2 cut(s) 195, 1184
TseI GCWGC 2 cut(s) 13, 533
Tsp45I GTSAC 2 cut(s) 195, 1184
TspDTI ATGAA 5 cut(s) 306, 536, 615, 987, 1302
TspGWI ACGGA 1 cut(s) 660
TspRI CASTG 3 cut(s) 204, 412, 970
VpaK11BI GGWCC 3 cut(s) 6, 161, 311
XapI RAATTY 3 cut(s) 247, 769, 1412
XceI RCATGY 1 cut(s) 759
XcmI CCANNNNNNNNNTGG 1 cut(s) 922
XspI CTAG 4 cut(s) 215, 279, 582, 1353
ZraI GACGTC 1 cut(s) 195
Zsp2I ATGCAT 1 cut(s) 142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.