Prupe.5G000400_v2.0.a1

BEST Arabidopsis thaliana protein match is hydroxyproline-rich glycoprotein family protein (TAIR AT5G52430.1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0012074
Physical Location & Seq
Forward (+)
168803 .. 172817
4015 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G000400.1

Sequence Viewer

Length: 1440 bp
ATGGGGTCGGAACAGAATAGATTCCCGCAGCAGGAACGGAGAAAGAGATGGGGAGGATGTTGGGGTGCATTCTCTTGTTTTGACTCACATAAAGGTGGAAAGCGTATAGTCCCCGCTTCTCGTATTCCTGAAGGCAATGCATCAGCAACGCAGCCAAATGGACCCCAAGCGGTTGGGCTGACCAACCAAGCCACATCACTAGCTCCATCTCTTTTGGCTCCGCCTTCTTCACCAGCATCATTTACAAATTCAGCACTTCCTTCTACAGCTCAGTCACCCAGTTGTTCCTTATTATTATCTGCCAACTCACCTGGAGGTCCTTCGTCAACAATGTATGCCACCGGGCCATATGCCAATGAAACACAACTGGTCTCTCCTCCTGTTTTCTCAACCTTCACAACTGAGCCGTCCACAGCTCCTCTCACTCCCCCACCAGAATTGGCTCACCTAACAACTCCCTCTTCCCCAGATGTGCCATTTGCTCGTTTCCTCTCATCTTCAGTAGACATTAAAACCACTGACAAGACCAATTACATTGCTGCAAATGATCTTCAAGCAACATATTCGCTCTATCCTGGAAGTCCTGCTAGTAGTCTTAGATCACCAATTTCAAGGGCATCAAATGACTGTTCATCATCTTTTCCTGAACGTGATTTCCCCCGACAGTGGGATCCCTCAGTTTCTCCCCAGAATGGCACCTATCCAAGGAGTGGTTCTGCCAGGCTGTTTGGGTATGACACAACTGGAGCCTCTGCGGCATCTCAAGACTCAAATTTTTTCTGTCCTGCTACATTTGCACAATTTTATCTGGATAATCCACCATTTCCTCATGCCGGTGGAAGGTTAAGCGTTTCCAAGGATTCAGATGTTTACTCAACTGGTGGAAATGGAAGTCAGAACAGACACAATAGAAGCCCCAAGCAAGATGTGGAAGAATTAGAAGCCTATAGAGCATCCTTTGGGTTCAGTGCTGATGAAATTATCACCACAACACAATATGTGGAGATTTCTGATGTAATGGATGACTCATTTACCATGACTCCTTTTACCTCTCATAAATTGCCTACCGAAGAACATATAGAACCTAAATCTGTCACCGAAGGACTTAAAGCACAGAAGACAAAGACAATCTTGCAGAGTCAGGACACTACTAAATCGGAATCAGATCTTGATGAGGGTGGAAGCTCTGACTTGCCGATATCATGTAATGGATATGAAGATCACAAATCATGGAGGCAGCCTGGTGATGTATCTCGATCAAGTACGCCTGGTCCTGGTGTCCGTGTTCTGGCCGACGAAGAAGACATTTTTTCAAAGATGGGGTCGTCTAAATTGAGCAGAAAGTATCAGCTGGGTCTATCAAGCTCAGATGCAGAAATTGACTATAGAAGGGGAAGAAGCTTACGAGAGAGGAAAGGAGAGTTTGCATGGCATGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

480

Amino Acids

51.69

Weight (kDa)

5.5

Isoelectric Point (pI)

71.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012074)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G76660 AT1G76660
fragaria_vesca FvH4_5g26800 FvH4_5g26800 FvH4_5g26800
malus_domestica MD00G1147000.v1.1 MD16G1282800.v1.1
prunus_persica Prupe.5G000400_v2.0.a1
pyrus_communis pycom16g26420
rosa_chinensis RchiOBHm_Chr7g0217711
rosa_laevigata RLG00000002445
rosa_multiflora Rmu_sc0000202.1_g000008
rosa_roxburghii Rroxscaffold_3G00241770
rosa_rugosa Rorug07G0170700
rosa_samantha Rh7AG311200 Rh7BG303100 Rh7CG330200 Rh7DG311000
rosa_wichuraiana Rw7G026420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 695
AccB7I CCANNNNNTGG 1 cut(s) 710
AccI GTMKAC 1 cut(s) 504
AciI CCGC 5 cut(s) 26, 114, 170, 221, 755
AclWI GGATC 2 cut(s) 665, 678
AcoI YGGCCR 1 cut(s) 1290
AcsI RAATTY 2 cut(s) 247, 772
AcuI CTGAAG 2 cut(s) 150, 483
AfaI GTAC 1 cut(s) 1264
AgsI TTSAA 3 cut(s) 554, 612, 1314
AjnI CCWGG 6 cut(s) 310, 574, 719, 1240, 1267, 1273
AjuI GAANNNNNNNTTGG 2 cut(s) 697, 729
AloI GAACNNNNNNTCC 2 cut(s) 639, 671
AluBI AGCT 7 cut(s) 203, 269, 416, 1185, 1351, 1365, 1401
AluI AGCT 7 cut(s) 203, 269, 416, 1185, 1351, 1365, 1401
Alw26I GTCTC 1 cut(s) 376
AlwI GGATC 2 cut(s) 665, 678
AoxI GGCC 2 cut(s) 344, 1290
ApeKI GCWGC 4 cut(s) 28, 151, 539, 1237
ApoI RAATTY 2 cut(s) 247, 772
ArsI GACNNNNNNTTYG 2 cut(s) 1307, 1339
Asp700I GAANNNNTTC 1 cut(s) 20
AspS9I GGNCC 4 cut(s) 161, 317, 344, 1271
AsuC2I CCSGG 1 cut(s) 343
AsuHPI GGTGA 8 cut(s) 222, 267, 300, 437, 594, 976, 1087, 1256
AvaII GGWCC 3 cut(s) 161, 317, 1271
BamHI GGATCC 1 cut(s) 670
BanI GGYRCC 1 cut(s) 695
BbsI GAAGAC 2 cut(s) 1124, 1308
BbvI GCAGC 4 cut(s) 40, 163, 526, 1249
BccI CCATC 3 cut(s) 42, 214, 1312
BceAI ACGGC 1 cut(s) 391
BcgI CGANNNNNNTGC 2 cut(s) 546, 580
BciT130I CCWGG 6 cut(s) 312, 576, 721, 1242, 1269, 1275
BcnI CCSGG 1 cut(s) 343
BcoDI GTCTC 1 cut(s) 376
BfaI CTAG 2 cut(s) 200, 588
BfmI CTRYAG 3 cut(s) 264, 946, 1384
BglI GCCNNNNNGGC 1 cut(s) 755
BglII AGATCT 1 cut(s) 1165
BisI GCNGC 5 cut(s) 29, 152, 540, 756, 1238
BlsI GCNGC 5 cut(s) 30, 153, 541, 757, 1239
Bme1390I CCNGG 7 cut(s) 312, 343, 576, 721, 1242, 1269, 1275
Bme18I GGWCC 3 cut(s) 161, 317, 1271
BmgT120I GGNCC 4 cut(s) 161, 317, 344, 1271
BmiI GGNNCC 5 cut(s) 163, 219, 672, 697, 748
BmrFI CCNGG 7 cut(s) 312, 343, 576, 721, 1242, 1269, 1275
BmrI ACTGGG 1 cut(s) 273
BmsI GCATC 6 cut(s) 149, 245, 626, 767, 962, 1360
BmuI ACTGGG 1 cut(s) 273
BpiI GAAGAC 2 cut(s) 1124, 1308
BpmI CTGGAG 2 cut(s) 333, 765
BpuEI CTTGAG 1 cut(s) 747
BpuMI CCSGG 1 cut(s) 343
BsaI GGTCTC 1 cut(s) 376
BsaJI CCNNGG 2 cut(s) 704, 855
BsaXI ACNNNNNCTCC 2 cut(s) 1024, 1054
Bse118I RCCGGY 1 cut(s) 833
Bse1I ACTGG 4 cut(s) 279, 372, 748, 883
Bse3DI GCAATG 2 cut(s) 142, 534
BseBI CCWGG 6 cut(s) 312, 576, 721, 1242, 1269, 1275
BseDI CCNNGG 2 cut(s) 704, 855
BseGI GGATG 3 cut(s) 62, 953, 1027
BseMI GCAATG 2 cut(s) 142, 534
BseMII CTCAG 4 cut(s) 284, 393, 690, 1380
BseNI ACTGG 4 cut(s) 279, 372, 748, 883
BseRI GAGGAG 2 cut(s) 366, 408
BseXI GCAGC 4 cut(s) 40, 163, 526, 1249
BseYI CCCAGC 1 cut(s) 1351
BshFI GGCC 2 cut(s) 346, 1292
BshNI GGYRCC 1 cut(s) 695
BsiSI CCGG 2 cut(s) 342, 834
BslFI GGGAC 1 cut(s) 95
BsmAI GTCTC 1 cut(s) 376
BsmFI GGGAC 1 cut(s) 95
BsmI GAATGC 1 cut(s) 68
BsnI GGCC 2 cut(s) 346, 1292
Bso31I GGTCTC 1 cut(s) 376
Bsp143I GATC 6 cut(s) 547, 599, 670, 1165, 1219, 1256
BspACI CCGC 5 cut(s) 26, 114, 170, 221, 755
BspANI GGCC 2 cut(s) 346, 1292
BspCNI CTCAG 4 cut(s) 283, 394, 689, 1379
BspLI GGNNCC 5 cut(s) 163, 219, 672, 697, 748
BspPI GGATC 2 cut(s) 665, 678
BspT107I GGYRCC 1 cut(s) 695
BspTNI GGTCTC 1 cut(s) 376
BsrDI GCAATG 2 cut(s) 142, 534
BsrFI RCCGGY 1 cut(s) 833
BsrI ACTGG 4 cut(s) 279, 372, 748, 883
BssAI RCCGGY 1 cut(s) 833
BssECI CCNNGG 2 cut(s) 704, 855
BssMI GATC 6 cut(s) 547, 599, 670, 1165, 1219, 1256
BssT1I CCWWGG 2 cut(s) 704, 855
Bst2UI CCWGG 6 cut(s) 312, 576, 721, 1242, 1269, 1275
Bst4CI ACNGT 2 cut(s) 629, 666
Bst6I CTCTTC 1 cut(s) 466
BstDEI CTNAG 5 cut(s) 270, 402, 596, 676, 1366
BstENI CCTNNNNNAGG 1 cut(s) 703
BstF5I GGATG 3 cut(s) 62, 953, 1027
BstKTI GATC 6 cut(s) 550, 602, 673, 1168, 1222, 1259
BstMAI GTCTC 1 cut(s) 376
BstMBI GATC 6 cut(s) 547, 599, 670, 1165, 1219, 1256
BstMWI GCNNNNNNNGC 3 cut(s) 755, 794, 950
BstNI CCWGG 6 cut(s) 312, 576, 721, 1242, 1269, 1275
BstSCI CCNGG 7 cut(s) 310, 341, 574, 719, 1240, 1267, 1273
BstSFI CTRYAG 3 cut(s) 264, 946, 1384
BstV1I GCAGC 4 cut(s) 40, 163, 526, 1249
BstV2I GAAGAC 2 cut(s) 1124, 1308
BstX2I RGATCY 2 cut(s) 670, 1165
BstXI CCANNNNNNTGG 1 cut(s) 173
BstYI RGATCY 2 cut(s) 670, 1165
BsuRI GGCC 2 cut(s) 346, 1292
BtsCI GGATG 3 cut(s) 62, 953, 1027
BtsIMutI CAGTG 3 cut(s) 516, 671, 973
Cfr10I RCCGGY 1 cut(s) 833
Cfr13I GGNCC 4 cut(s) 161, 317, 344, 1271
Csp6I GTAC 1 cut(s) 1263
CspCI CAANNNNNGTGG 2 cut(s) 420, 455
CviAII CATG 6 cut(s) 830, 1036, 1203, 1230, 1428, 1433
CviQI GTAC 1 cut(s) 1263
DdeI CTNAG 5 cut(s) 270, 402, 596, 676, 1366
DpnI GATC 6 cut(s) 549, 601, 672, 1167, 1221, 1258
DpnII GATC 6 cut(s) 547, 599, 670, 1165, 1219, 1256
EaeI YGGCCR 1 cut(s) 1290
Eam1104I CTCTTC 1 cut(s) 466
EarI CTCTTC 1 cut(s) 466
EciI GGCGGA 1 cut(s) 210
Eco130I CCWWGG 2 cut(s) 704, 855
Eco31I GGTCTC 1 cut(s) 376
Eco32I GATATC 1 cut(s) 1200
Eco47I GGWCC 3 cut(s) 161, 317, 1271
Eco57I CTGAAG 2 cut(s) 150, 483
EcoNI CCTNNNNNAGG 1 cut(s) 703
EcoO109I RGGNCCY 1 cut(s) 317
EcoRII CCWGG 6 cut(s) 310, 574, 719, 1240, 1267, 1273
EcoRV GATATC 1 cut(s) 1200
EcoT14I CCWWGG 2 cut(s) 704, 855
EcoT22I ATGCAT 1 cut(s) 142
ErhI CCWWGG 2 cut(s) 704, 855
FaeI CATG 6 cut(s) 833, 1039, 1206, 1233, 1431, 1436
FalI AAGNNNNNCTT 2 cut(s) 1115, 1147
FaqI GGGAC 1 cut(s) 95
FatI CATG 6 cut(s) 829, 1035, 1202, 1229, 1427, 1432
FauI CCCGC 2 cut(s) 33, 121
FauNDI CATATG 1 cut(s) 349
FblI GTMKAC 1 cut(s) 504
Fnu4HI GCNGC 5 cut(s) 29, 152, 540, 756, 1238
FokI GGATG 3 cut(s) 69, 940, 1034
Fsp4HI GCNGC 5 cut(s) 29, 152, 540, 756, 1238
FspBI CTAG 2 cut(s) 200, 588
GluI GCNGC 5 cut(s) 29, 152, 540, 756, 1238
GsaI CCCAGC 1 cut(s) 1355
GsuI CTGGAG 2 cut(s) 333, 765
HaeIII GGCC 2 cut(s) 346, 1292
HapII CCGG 2 cut(s) 342, 834
Hin1II CATG 6 cut(s) 833, 1039, 1206, 1233, 1431, 1436
HincII GTYRAC 1 cut(s) 327
HindII GTYRAC 1 cut(s) 327
HindIII AAGCTT 1 cut(s) 1399
HinfI GANTC 8 cut(s) 21, 83, 767, 860, 1025, 1039, 1138, 1160
HpaII CCGG 2 cut(s) 342, 834
HphI GGTGA 8 cut(s) 222, 267, 300, 437, 594, 976, 1087, 1256
Hpy166II GTNNAC 4 cut(s) 327, 411, 505, 871
Hpy188I TCNGA 8 cut(s) 10, 865, 897, 1012, 1159, 1165, 1189, 1369
Hpy188III TCNNGA 7 cut(s) 128, 644, 764, 809, 1142, 1169, 1254
Hpy8I GTNNAC 4 cut(s) 327, 411, 505, 871
Hpy99I CGWCG 1 cut(s) 1298
HpyAV CCTTC 8 cut(s) 125, 234, 270, 330, 403, 834, 1094, 1383
HpyCH4III ACNGT 2 cut(s) 629, 666
HpyCH4IV ACGT 1 cut(s) 649
HpyCH4V TGCA 7 cut(s) 68, 140, 542, 797, 1135, 1373, 1427
HpyF10VI GCNNNNNNNGC 3 cut(s) 755, 794, 950
HpyF3I CTNAG 5 cut(s) 270, 402, 596, 676, 1366
HpySE526I ACGT 1 cut(s) 649
Hsp92II CATG 6 cut(s) 833, 1039, 1206, 1233, 1431, 1436
Kzo9I GATC 6 cut(s) 547, 599, 670, 1165, 1219, 1256
LmnI GCTCC 4 cut(s) 208, 223, 421, 746
Lsp1109I GCAGC 4 cut(s) 40, 163, 526, 1249
LweI GCATC 6 cut(s) 149, 245, 626, 767, 962, 1360
MaeI CTAG 2 cut(s) 200, 588
MaeII ACGT 1 cut(s) 649
MaeIII GTNAC 2 cut(s) 273, 1093
MalI GATC 6 cut(s) 549, 601, 672, 1167, 1221, 1258
MboI GATC 6 cut(s) 547, 599, 670, 1165, 1219, 1256
MflI RGATCY 2 cut(s) 670, 1165
MlyI GAGTC 5 cut(s) 77, 761, 1019, 1033, 1147
Mph1103I ATGCAT 1 cut(s) 142
MroXI GAANNNNTTC 1 cut(s) 20
MseI TTAA 3 cut(s) 510, 845, 1107
MslI CAYNNNNRTG 2 cut(s) 93, 834
MspA1I CMGCKG 1 cut(s) 1351
MspI CCGG 2 cut(s) 342, 834
MspR9I CCNGG 7 cut(s) 312, 343, 576, 721, 1242, 1269, 1275
Mva1269I GAATGC 1 cut(s) 68
MvaI CCWGG 6 cut(s) 312, 576, 721, 1242, 1269, 1275
MwoI GCNNNNNNNGC 3 cut(s) 755, 794, 950
NciI CCSGG 1 cut(s) 343
NdeI CATATG 1 cut(s) 349
NdeII GATC 6 cut(s) 547, 599, 670, 1165, 1219, 1256
NlaIII CATG 6 cut(s) 833, 1039, 1206, 1233, 1431, 1436
NlaIV GGNNCC 5 cut(s) 163, 219, 672, 697, 748
NmuCI GTSAC 2 cut(s) 273, 1093
NsiI ATGCAT 1 cut(s) 142
PctI GAATGC 1 cut(s) 68
PdmI GAANNNNTTC 1 cut(s) 20
PfeI GAWTC 3 cut(s) 21, 860, 1160
PflMI CCANNNNNTGG 1 cut(s) 710
PfoI TCCNGGA 1 cut(s) 574
PkrI GCNGC 5 cut(s) 30, 153, 541, 757, 1239
PleI GAGTC 5 cut(s) 77, 761, 1019, 1033, 1146
PpsI GAGTC 5 cut(s) 77, 761, 1019, 1033, 1146
PpuMI RGGWCCY 1 cut(s) 317
Psp5II RGGWCCY 1 cut(s) 317
Psp6I CCWGG 6 cut(s) 310, 574, 719, 1240, 1267, 1273
PspFI CCCAGC 1 cut(s) 1351
PspGI CCWGG 6 cut(s) 310, 574, 719, 1240, 1267, 1273
PspN4I GGNNCC 5 cut(s) 163, 219, 672, 697, 748
PspPI GGNCC 4 cut(s) 161, 317, 344, 1271
PspPPI RGGWCCY 1 cut(s) 317
PsuI RGATCY 2 cut(s) 670, 1165
PvuII CAGCTG 1 cut(s) 1351
RsaI GTAC 1 cut(s) 1264
RsaNI GTAC 1 cut(s) 1263
RseI CAYNNNNRTG 2 cut(s) 93, 834
SaqAI TTAA 3 cut(s) 510, 845, 1107
SatI GCNGC 5 cut(s) 29, 152, 540, 756, 1238
Sau3AI GATC 6 cut(s) 547, 599, 670, 1165, 1219, 1256
Sau96I GGNCC 4 cut(s) 161, 317, 344, 1271
SchI GAGTC 5 cut(s) 77, 761, 1019, 1033, 1147
ScrFI CCNGG 7 cut(s) 312, 343, 576, 721, 1242, 1269, 1275
SfaNI GCATC 6 cut(s) 149, 245, 626, 767, 962, 1360
SfcI CTRYAG 3 cut(s) 264, 946, 1384
SinI GGWCC 3 cut(s) 161, 317, 1271
SmiMI CAYNNNNRTG 2 cut(s) 93, 834
SmlI CTYRAG 1 cut(s) 762
SmoI CTYRAG 1 cut(s) 762
SsiI CCGC 5 cut(s) 26, 114, 170, 221, 755
SspMI CTAG 2 cut(s) 200, 588
StyD4I CCNGG 7 cut(s) 310, 341, 574, 719, 1240, 1267, 1273
StyI CCWWGG 2 cut(s) 704, 855
TaaI ACNGT 2 cut(s) 629, 666
TaiI ACGT 1 cut(s) 652
TaqI TCGA 1 cut(s) 1255
TauI GCSGC 1 cut(s) 758
TfiI GAWTC 3 cut(s) 21, 860, 1160
Tru1I TTAA 3 cut(s) 510, 845, 1107
Tru9I TTAA 3 cut(s) 510, 845, 1107
TscAI CASTG 3 cut(s) 523, 671, 973
TseFI GTSAC 2 cut(s) 273, 1093
TseI GCWGC 4 cut(s) 28, 151, 539, 1237
Tsp45I GTSAC 2 cut(s) 273, 1093
TspDTI ATGAA 4 cut(s) 372, 621, 990, 1230
TspGWI ACGGA 2 cut(s) 52, 1271
TspRI CASTG 3 cut(s) 523, 671, 973
Van91I CCANNNNNTGG 1 cut(s) 710
VpaK11BI GGWCC 3 cut(s) 161, 317, 1271
XagI CCTNNNNNAGG 1 cut(s) 703
XapI RAATTY 2 cut(s) 247, 772
XcmI CCANNNNNNNNNTGG 1 cut(s) 925
XmiI GTMKAC 1 cut(s) 504
XmnI GAANNNNTTC 1 cut(s) 20
XspI CTAG 2 cut(s) 200, 588
Zsp2I ATGCAT 1 cut(s) 142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.