MD00G1165600.v1.1

Prokaryotic RING finger family 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0009682
Physical Location & Seq
Forward (+)
37697365 .. 37699583
2219 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1165600.v1.1.491

Sequence Viewer

Length: 759 bp
ATGTGGCAAGCTCATGCCTGCAAGTCTTCTTATGGAGAATCCATCAAAGCTCTTGAAGCTGATATCCAACATGCCAATACCTTGGCAGCTGCTCTTCCCAGAGATTATGGTGGGAACTGTATCCAAATGAGATTGTCTTACAGCCCCTTTGCACCTTTCTTCCTGTATCTCATTGAATGGATGGATTGTAGTTGCACTGATGTACTTCCCAATTATTTAGGCCTTCTCCACATCCTTGTACACAAGGTATATGTCGATGGGATGCCTTCATTGTCCTCCAAAGAACAGAAGGTCACTTTAAGGGAATTTTACGCTGTAATATACCCTTATCTTAGGCAACTTGAAGGCGAATTTAACGAACTGGAAGATAATAATAATAAGAGAAGTCGATGCACAGATGTTTTGGGCAGAAAGAGGATGGAAGACCAAAGGAAGCTTTCGGATAAAGATCTTGAGAGAGAAGATGAATGTGGGATATGCATGGAAAACTGTACAAAGATGGTGTTGCCTGACTGTGGGCATTCCATGTGCCTCACCTGCTTCCATGATTGGAATGCAAGATCGAAATCCTGCCCTTTTTGCCGCGGCAGCCTAAAGAGTGTTAGCTCCAGAGATTTGTGGGTTCTCACTAGCATCGGTGATGTTATTGATGCAGTAACCCTTGCGCAGGAGAACCTAAGACGTTTCTATCTTTATATTGAAAATCTACCTGTAATTGTGCCGGCAACGCATATTGTGGTATACGATTACATGCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000122 GO:0000151 GO:0000209 GO:0000726 GO:0000781 GO:0001775 GO:0002200 GO:0002204 GO:0002208 GO:0002250 GO:0002252 GO:0002263 GO:0002285 GO:0002312 GO:0002366 GO:0002376 GO:0002377 GO:0002381 GO:0002440 GO:0002443 GO:0002449 GO:0002460 GO:0002520 GO:0002562 GO:0003006 GO:0003674 GO:0003682 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006281 GO:0006282 GO:0006302 GO:0006303 GO:0006310 GO:0006325 GO:0006338 GO:0006355 GO:0006357 GO:0006464 GO:0006508 GO:0006511 GO:0006725 GO:0006807 GO:0006950 GO:0006955 GO:0006974 GO:0006996 GO:0007275 GO:0007276 GO:0007281 GO:0007283 GO:0007286 GO:0008150 GO:0008152 GO:0008270 GO:0009056 GO:0009057 GO:0009314 GO:0009628 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009987 GO:0010212 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010629 GO:0016043 GO:0016064 GO:0016444 GO:0016445 GO:0016447 GO:0016567 GO:0016569 GO:0016570 GO:0016574 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019724 GO:0019787 GO:0019941 GO:0019953 GO:0022412 GO:0022414 GO:0030154 GO:0030163 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0032446 GO:0032501 GO:0032502 GO:0032504 GO:0032784 GO:0032785 GO:0032991 GO:0033522 GO:0033523 GO:0033554 GO:0034243 GO:0034244 GO:0034641 GO:0034728 GO:0035861 GO:0036211 GO:0042113 GO:0042393 GO:0042802 GO:0042803 GO:0043044 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0043486 GO:0043632 GO:0043933 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0044703 GO:0045190 GO:0045321 GO:0045739 GO:0045892 GO:0045934 GO:0045935 GO:0046483 GO:0046649 GO:0046872 GO:0046914 GO:0046983 GO:0048232 GO:0048468 GO:0048515 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048609 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051276 GO:0051603 GO:0051704 GO:0051716 GO:0060255 GO:0065007 GO:0070534 GO:0070535 GO:0070647 GO:0070936 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0080134 GO:0080135 GO:0090304 GO:0090734 GO:0098687 GO:0140096 GO:1901360 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:1990234 GO:2000112 GO:2000113 GO:2001020 GO:2001022 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

253

Amino Acids

28.98

Weight (kDa)

5.66

Isoelectric Point (pI)

65.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 154 - 200 1.7e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 155 - 195 4.7e-08 Ring finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 545
Acc16I TGCGCA 1 cut(s) 666
Acc36I ACCTGC 1 cut(s) 545
AccI GTMKAC 1 cut(s) 741
AccII CGCG 1 cut(s) 585
AciI CCGC 2 cut(s) 583, 585
AcsI RAATTY 2 cut(s) 305, 350
AfaI GTAC 3 cut(s) 204, 240, 493
AfiI CCNNNNNNNGG 2 cut(s) 515, 667
AgsI TTSAA 4 cut(s) 56, 176, 344, 701
AluBI AGCT 6 cut(s) 11, 50, 59, 89, 436, 606
AluI AGCT 6 cut(s) 11, 50, 59, 89, 436, 606
AoxI GGCC 1 cut(s) 220
ApeKI GCWGC 3 cut(s) 86, 89, 588
ApoI RAATTY 2 cut(s) 305, 350
AspLEI GCGC 1 cut(s) 667
AsuHPI GGTGA 2 cut(s) 526, 650
BbsI GAAGAC 2 cut(s) 18, 429
BbvI GCAGC 3 cut(s) 76, 98, 600
BccI CCATC 5 cut(s) 50, 175, 251, 412, 493
BciVI GTATCC 1 cut(s) 131
BfaI CTAG 1 cut(s) 630
BfuAI ACCTGC 1 cut(s) 545
BfuI GTATCC 1 cut(s) 131
BglII AGATCT 1 cut(s) 448
BisI GCNGC 5 cut(s) 87, 90, 583, 586, 589
BlsI GCNGC 5 cut(s) 88, 91, 584, 587, 590
BmsI GCATC 4 cut(s) 252, 380, 640, 642
BpiI GAAGAC 2 cut(s) 18, 429
BpmI CTGGAG 1 cut(s) 592
BpuEI CTTGAG 1 cut(s) 473
BsaBI GATNNNNATC 2 cut(s) 447, 565
BsaJI CCNNGG 2 cut(s) 81, 583
Bsc4I CCNNNNNNNGG 2 cut(s) 515, 667
Bse118I RCCGGY 1 cut(s) 721
Bse1I ACTGG 1 cut(s) 366
Bse8I GATNNNNATC 2 cut(s) 447, 565
BseDI CCNNGG 2 cut(s) 81, 583
BseGI GGATG 4 cut(s) 186, 231, 267, 423
BseJI GATNNNNATC 2 cut(s) 447, 565
BseLI CCNNNNNNNGG 2 cut(s) 515, 667
BseNI ACTGG 1 cut(s) 366
BseXI GCAGC 3 cut(s) 76, 98, 600
Bsh1236I CGCG 1 cut(s) 585
BshFI GGCC 1 cut(s) 222
BsiSI CCGG 1 cut(s) 722
BslI CCNNNNNNNGG 2 cut(s) 515, 667
BsmI GAATGC 2 cut(s) 520, 559
BsnI GGCC 1 cut(s) 222
Bsp1407I TGTACA 2 cut(s) 238, 491
Bsp143I GATC 2 cut(s) 448, 560
BspACI CCGC 2 cut(s) 583, 585
BspANI GGCC 1 cut(s) 222
BspFNI CGCG 1 cut(s) 585
BspMI ACCTGC 1 cut(s) 545
BspQI GCTCTTC 1 cut(s) 99
BsrFI RCCGGY 1 cut(s) 721
BsrGI TGTACA 2 cut(s) 238, 491
BsrI ACTGG 1 cut(s) 366
BssAI RCCGGY 1 cut(s) 721
BssECI CCNNGG 2 cut(s) 81, 583
BssMI GATC 2 cut(s) 448, 560
BssNAI GTATAC 1 cut(s) 742
BssT1I CCWWGG 1 cut(s) 81
Bst1107I GTATAC 1 cut(s) 742
Bst4CI ACNGT 3 cut(s) 119, 491, 515
Bst6I CTCTTC 1 cut(s) 99
BstAUI TGTACA 2 cut(s) 238, 491
BstC8I GCNNGC 3 cut(s) 9, 19, 723
BstDEI CTNAG 2 cut(s) 332, 677
BstDSI CCRYGG 1 cut(s) 583
BstENI CCTNNNNNAGG 1 cut(s) 665
BstF5I GGATG 4 cut(s) 186, 231, 267, 423
BstFNI CGCG 1 cut(s) 585
BstHHI GCGC 1 cut(s) 667
BstKTI GATC 2 cut(s) 451, 563
BstMBI GATC 2 cut(s) 448, 560
BstMWI GCNNNNNNNGC 5 cut(s) 56, 537, 579, 588, 727
BstNSI RCATGY 2 cut(s) 74, 754
BstUI CGCG 1 cut(s) 585
BstV1I GCAGC 3 cut(s) 76, 98, 600
BstV2I GAAGAC 2 cut(s) 18, 429
BstX2I RGATCY 1 cut(s) 448
BstXI CCANNNNNNTGG 1 cut(s) 82
BstYI RGATCY 1 cut(s) 448
BstZ17I GTATAC 1 cut(s) 742
BsuI GTATCC 1 cut(s) 131
BsuRI GGCC 1 cut(s) 222
BtgI CCRYGG 1 cut(s) 583
BtsCI GGATG 4 cut(s) 186, 231, 267, 423
BtsIMutI CAGTG 1 cut(s) 195
BveI ACCTGC 1 cut(s) 545
Cac8I GCNNGC 3 cut(s) 9, 19, 723
CfoI GCGC 1 cut(s) 667
Cfr10I RCCGGY 1 cut(s) 721
Cfr42I CCGCGG 1 cut(s) 586
Csp6I GTAC 3 cut(s) 203, 239, 492
CviAII CATG 6 cut(s) 14, 71, 481, 526, 545, 751
CviJI RGCY 9 cut(s) 11, 50, 59, 89, 144, 222, 436, 591, 606
CviKI_1 RGCY 9 cut(s) 11, 50, 59, 89, 144, 222, 436, 591, 606
CviQI GTAC 3 cut(s) 203, 239, 492
DdeI CTNAG 2 cut(s) 332, 677
DpnI GATC 2 cut(s) 450, 562
DpnII GATC 2 cut(s) 448, 560
Eam1104I CTCTTC 1 cut(s) 99
EarI CTCTTC 1 cut(s) 99
Eco130I CCWWGG 1 cut(s) 81
Eco147I AGGCCT 1 cut(s) 222
Eco32I GATATC 1 cut(s) 64
EcoNI CCTNNNNNAGG 1 cut(s) 665
EcoRV GATATC 1 cut(s) 64
EcoT14I CCWWGG 1 cut(s) 81
EcoT22I ATGCAT 1 cut(s) 482
ErhI CCWWGG 1 cut(s) 81
FaeI CATG 6 cut(s) 17, 74, 484, 529, 548, 754
FatI CATG 6 cut(s) 13, 70, 480, 525, 544, 750
FblI GTMKAC 1 cut(s) 741
Fnu4HI GCNGC 5 cut(s) 87, 90, 583, 586, 589
FokI GGATG 4 cut(s) 193, 218, 274, 430
Fsp4HI GCNGC 5 cut(s) 87, 90, 583, 586, 589
FspBI CTAG 1 cut(s) 630
FspI TGCGCA 1 cut(s) 666
GlaI GCGC 1 cut(s) 666
GluI GCNGC 5 cut(s) 87, 90, 583, 586, 589
GsuI CTGGAG 1 cut(s) 592
HaeIII GGCC 1 cut(s) 222
HapII CCGG 1 cut(s) 722
HhaI GCGC 1 cut(s) 667
Hin1II CATG 6 cut(s) 17, 74, 484, 529, 548, 754
Hin6I GCGC 1 cut(s) 665
HinP1I GCGC 1 cut(s) 665
HindIII AAGCTT 1 cut(s) 434
HinfI GANTC 1 cut(s) 38
HpaII CCGG 1 cut(s) 722
HphI GGTGA 2 cut(s) 526, 650
Hpy166II GTNNAC 2 cut(s) 241, 742
Hpy188I TCNGA 2 cut(s) 442, 758
Hpy188III TCNNGA 3 cut(s) 53, 452, 609
Hpy8I GTNNAC 2 cut(s) 241, 742
HpyAV CCTTC 4 cut(s) 233, 276, 283, 338
HpyCH4III ACNGT 3 cut(s) 119, 491, 515
HpyCH4IV ACGT 1 cut(s) 682
HpyCH4V TGCA 7 cut(s) 21, 152, 195, 393, 480, 557, 653
HpyF10VI GCNNNNNNNGC 5 cut(s) 56, 537, 579, 588, 727
HpyF3I CTNAG 2 cut(s) 332, 677
HpySE526I ACGT 1 cut(s) 682
Hsp92II CATG 6 cut(s) 17, 74, 484, 529, 548, 754
HspAI GCGC 1 cut(s) 665
KroI GCCGGC 1 cut(s) 721
KroNI GCCGGC 1 cut(s) 723
KspI CCGCGG 1 cut(s) 586
Kzo9I GATC 2 cut(s) 448, 560
LguI GCTCTTC 1 cut(s) 99
LmnI GCTCC 1 cut(s) 611
Lsp1109I GCAGC 3 cut(s) 76, 98, 600
LweI GCATC 4 cut(s) 252, 380, 640, 642
MaeI CTAG 1 cut(s) 630
MaeII ACGT 1 cut(s) 682
MaeIII GTNAC 2 cut(s) 292, 655
MalI GATC 2 cut(s) 450, 562
MboI GATC 2 cut(s) 448, 560
MboII GAAGA 6 cut(s) 18, 86, 151, 377, 434, 473
MflI RGATCY 1 cut(s) 448
MluCI AATT 4 cut(s) 211, 305, 350, 714
MmeI TCCRAC 1 cut(s) 91
MnlI CCTC 3 cut(s) 286, 408, 542
Mph1103I ATGCAT 1 cut(s) 482
MroNI GCCGGC 1 cut(s) 721
MseI TTAA 2 cut(s) 299, 354
MspA1I CMGCKG 2 cut(s) 89, 585
MspI CCGG 1 cut(s) 722
Mva1269I GAATGC 2 cut(s) 520, 559
MvnI CGCG 1 cut(s) 585
MwoI GCNNNNNNNGC 5 cut(s) 56, 537, 579, 588, 727
NaeI GCCGGC 1 cut(s) 723
NdeII GATC 2 cut(s) 448, 560
NgoMIV GCCGGC 1 cut(s) 721
NlaIII CATG 6 cut(s) 17, 74, 484, 529, 548, 754
NmuCI GTSAC 1 cut(s) 292
NsbI TGCGCA 1 cut(s) 666
NsiI ATGCAT 1 cut(s) 482
NspI RCATGY 2 cut(s) 74, 754
PaqCI CACCTGC 1 cut(s) 545
PceI AGGCCT 1 cut(s) 222
PciSI GCTCTTC 1 cut(s) 99
PctI GAATGC 2 cut(s) 520, 559
PdiI GCCGGC 1 cut(s) 723
PfeI GAWTC 1 cut(s) 38
PkrI GCNGC 5 cut(s) 88, 91, 584, 587, 590
PsuI RGATCY 1 cut(s) 448
PvuII CAGCTG 1 cut(s) 89
RsaI GTAC 3 cut(s) 204, 240, 493
RsaNI GTAC 3 cut(s) 203, 239, 492
SacII CCGCGG 1 cut(s) 586
SapI GCTCTTC 1 cut(s) 99
SaqAI TTAA 2 cut(s) 299, 354
SatI GCNGC 5 cut(s) 87, 90, 583, 586, 589
Sau3AI GATC 2 cut(s) 448, 560
SfaNI GCATC 4 cut(s) 252, 380, 640, 642
Sfr303I CCGCGG 1 cut(s) 586
SgrBI CCGCGG 1 cut(s) 586
SmlI CTYRAG 1 cut(s) 452
SmoI CTYRAG 1 cut(s) 452
Sse9I AATT 4 cut(s) 211, 305, 350, 714
SseBI AGGCCT 1 cut(s) 222
SsiI CCGC 2 cut(s) 583, 585
SspMI CTAG 1 cut(s) 630
StuI AGGCCT 1 cut(s) 222
StyI CCWWGG 1 cut(s) 81
TaaI ACNGT 3 cut(s) 119, 491, 515
TaiI ACGT 1 cut(s) 685
TaqI TCGA 3 cut(s) 255, 388, 563
TasI AATT 4 cut(s) 211, 305, 350, 714
TatI WGTACW 3 cut(s) 202, 238, 491
TauI GCSGC 2 cut(s) 585, 588
TfiI GAWTC 1 cut(s) 38
Tru1I TTAA 2 cut(s) 299, 354
Tru9I TTAA 2 cut(s) 299, 354
TscAI CASTG 1 cut(s) 202
TseFI GTSAC 1 cut(s) 292
TseI GCWGC 3 cut(s) 86, 89, 588
Tsp45I GTSAC 1 cut(s) 292
TspDTI ATGAA 2 cut(s) 258, 480
TspRI CASTG 1 cut(s) 202
XagI CCTNNNNNAGG 1 cut(s) 665
XapI RAATTY 2 cut(s) 305, 350
XceI RCATGY 2 cut(s) 74, 754
XmiI GTMKAC 1 cut(s) 741
XspI CTAG 1 cut(s) 630
Zsp2I ATGCAT 1 cut(s) 482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.