pycom16g09220

Prokaryotic RING finger family 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0009682
Physical Location & Seq
Forward (+)
6185306 .. 6186787
1482 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g09220.4

Sequence Viewer

Length: 762 bp
ATGTGGCAAGCTCAGGCCTGCAAGTCTTCTTATGGAGAATCCATCAAAGCTCTTGAAGCTGATATCCAACATGCCAATACCTTGGCAGCTGCTCTTCCTGGAGATTATGGTGGGAACTGTATCCAAATGAGATTGTCTTATAGCCCCTTTGCACCTTTCTTCCTGTATCTCATTGAATGGATGGATTGTCGTTGCACTGATATACTTCCCAATTATTTAGGCCTTCTCCACATCCTTGTATACAAGGATATTGTACAGGATGGAATGCCTTCGTTGTCCTCCAAAGAACAGGTCGCCACTTTAAGGGAATTTTATGCTGTAATATACCCTTATCTTAGACAACTCGAAGGTGAATTTAACGAACTGGAAGATAATAATAATAAGAGAAGCCGATGCACAGATGTTTTGGGCAGAAAGAGGATGGAAGAGCGGAGGAAGCATTCGGATAACGATCTAGAGAGAGATGATGAATGTGGGATATGCATGGAAAACTGTACAAAAATGGTGTTGCCCAACTGTGGACATTCCATGTGCATCACCTGCTTCCATGATTGGAATGCAAGATCGAAATCCTGCCCTTTTTGCCGCGGCGGCCTAAAGAGAGTCAGCTCCAGAGATTTGTGGGTTCTCACTAGCATCGGCGATGTTATTGATGCAGTAACCCTTGCGAAGGAGAACCTAAGACGTTTCTATCTTTATATTGAAAATCTACCTGTTGTTGTGCCGGCAACACCTATTGTGGTATATGATTACATGCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000122 GO:0000151 GO:0000209 GO:0000726 GO:0000781 GO:0001775 GO:0002200 GO:0002204 GO:0002208 GO:0002250 GO:0002252 GO:0002263 GO:0002285 GO:0002312 GO:0002366 GO:0002376 GO:0002377 GO:0002381 GO:0002440 GO:0002443 GO:0002449 GO:0002460 GO:0002520 GO:0002562 GO:0003006 GO:0003674 GO:0003682 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006281 GO:0006282 GO:0006302 GO:0006303 GO:0006310 GO:0006325 GO:0006338 GO:0006355 GO:0006357 GO:0006464 GO:0006508 GO:0006511 GO:0006725 GO:0006807 GO:0006950 GO:0006955 GO:0006974 GO:0006996 GO:0007275 GO:0007276 GO:0007281 GO:0007283 GO:0007286 GO:0008150 GO:0008152 GO:0008270 GO:0009056 GO:0009057 GO:0009314 GO:0009628 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009987 GO:0010212 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010629 GO:0016043 GO:0016064 GO:0016444 GO:0016445 GO:0016447 GO:0016567 GO:0016569 GO:0016570 GO:0016574 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019724 GO:0019787 GO:0019941 GO:0019953 GO:0022412 GO:0022414 GO:0030154 GO:0030163 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0032446 GO:0032501 GO:0032502 GO:0032504 GO:0032784 GO:0032785 GO:0032991 GO:0033522 GO:0033523 GO:0033554 GO:0034243 GO:0034244 GO:0034641 GO:0034728 GO:0035861 GO:0036211 GO:0042113 GO:0042393 GO:0042802 GO:0042803 GO:0043044 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0043486 GO:0043632 GO:0043933 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0044703 GO:0045190 GO:0045321 GO:0045739 GO:0045892 GO:0045934 GO:0045935 GO:0046483 GO:0046649 GO:0046872 GO:0046914 GO:0046983 GO:0048232 GO:0048468 GO:0048515 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048609 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051276 GO:0051603 GO:0051704 GO:0051716 GO:0060255 GO:0065007 GO:0070534 GO:0070535 GO:0070647 GO:0070936 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0080134 GO:0080135 GO:0090304 GO:0090734 GO:0098687 GO:0140096 GO:1901360 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:1990234 GO:2000112 GO:2000113 GO:2001020 GO:2001022 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

254

Amino Acids

29.04

Weight (kDa)

5.6

Isoelectric Point (pI)

68.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 548
Acc36I ACCTGC 1 cut(s) 548
AccBSI CCGCTC 1 cut(s) 430
AccI GTMKAC 1 cut(s) 240
AccII CGCG 1 cut(s) 588
AciI CCGC 4 cut(s) 430, 586, 588, 591
AcsI RAATTY 2 cut(s) 308, 353
AfaI GTAC 2 cut(s) 255, 496
AfiI CCNNNNNNNGG 3 cut(s) 303, 518, 670
AgsI TTSAA 3 cut(s) 56, 176, 704
AjnI CCWGG 1 cut(s) 97
AluBI AGCT 5 cut(s) 11, 50, 59, 89, 609
AluI AGCT 5 cut(s) 11, 50, 59, 89, 609
AoxI GGCC 3 cut(s) 15, 220, 592
ApeKI GCWGC 2 cut(s) 86, 89
ApoI RAATTY 2 cut(s) 308, 353
ArsI GACNNNNNNTTYG 2 cut(s) 276, 308
Asp700I GAANNNNTTC 1 cut(s) 268
AsuHPI GGTGA 2 cut(s) 362, 529
BbsI GAAGAC 1 cut(s) 18
BbvI GCAGC 2 cut(s) 76, 98
BccI CCATC 4 cut(s) 50, 175, 254, 415
BciT130I CCWGG 1 cut(s) 99
BciVI GTATCC 1 cut(s) 131
BfaI CTAG 2 cut(s) 455, 633
BfuAI ACCTGC 1 cut(s) 548
BfuI GTATCC 1 cut(s) 131
BglI GCCNNNNNGGC 1 cut(s) 591
BisI GCNGC 5 cut(s) 87, 90, 586, 589, 592
BlsI GCNGC 5 cut(s) 88, 91, 587, 590, 593
Bme1390I CCNGG 1 cut(s) 99
BmrFI CCNGG 1 cut(s) 99
BmsI GCATC 4 cut(s) 383, 543, 643, 645
BpiI GAAGAC 1 cut(s) 18
BpmI CTGGAG 2 cut(s) 120, 595
Bpu10I CCTNAGC 1 cut(s) 12
BsaBI GATNNNNATC 2 cut(s) 450, 568
BsaJI CCNNGG 2 cut(s) 81, 586
Bsc4I CCNNNNNNNGG 3 cut(s) 303, 518, 670
Bse118I RCCGGY 1 cut(s) 724
Bse1I ACTGG 1 cut(s) 369
Bse8I GATNNNNATC 2 cut(s) 450, 568
BseBI CCWGG 1 cut(s) 99
BseDI CCNNGG 2 cut(s) 81, 586
BseGI GGATG 4 cut(s) 186, 231, 265, 426
BseJI GATNNNNATC 2 cut(s) 450, 568
BseLI CCNNNNNNNGG 3 cut(s) 303, 518, 670
BseMII CTCAG 1 cut(s) 26
BseNI ACTGG 1 cut(s) 369
BseXI GCAGC 2 cut(s) 76, 98
Bsh1236I CGCG 1 cut(s) 588
BshFI GGCC 3 cut(s) 17, 222, 594
BsiSI CCGG 1 cut(s) 725
BslI CCNNNNNNNGG 3 cut(s) 303, 518, 670
BsmI GAATGC 3 cut(s) 270, 439, 562
BsnI GGCC 3 cut(s) 17, 222, 594
Bsp1407I TGTACA 2 cut(s) 253, 494
Bsp143I GATC 2 cut(s) 451, 563
BspACI CCGC 4 cut(s) 430, 586, 588, 591
BspANI GGCC 3 cut(s) 17, 222, 594
BspCNI CTCAG 1 cut(s) 25
BspFNI CGCG 1 cut(s) 588
BspMI ACCTGC 1 cut(s) 548
BspQI GCTCTTC 2 cut(s) 99, 420
BsrBI CCGCTC 1 cut(s) 430
BsrFI RCCGGY 1 cut(s) 724
BsrGI TGTACA 2 cut(s) 253, 494
BsrI ACTGG 1 cut(s) 369
BssAI RCCGGY 1 cut(s) 724
BssECI CCNNGG 2 cut(s) 81, 586
BssMI GATC 2 cut(s) 451, 563
BssNAI GTATAC 1 cut(s) 241
BssT1I CCWWGG 1 cut(s) 81
Bst1107I GTATAC 1 cut(s) 241
Bst2UI CCWGG 1 cut(s) 99
Bst4CI ACNGT 3 cut(s) 119, 494, 518
Bst6I CTCTTC 2 cut(s) 99, 420
BstAPI GCANNNNNTGC 1 cut(s) 540
BstAUI TGTACA 2 cut(s) 253, 494
BstC8I GCNNGC 3 cut(s) 9, 19, 726
BstDEI CTNAG 3 cut(s) 12, 335, 680
BstDSI CCRYGG 1 cut(s) 586
BstENI CCTNNNNNAGG 1 cut(s) 668
BstF5I GGATG 4 cut(s) 186, 231, 265, 426
BstFNI CGCG 1 cut(s) 588
BstKTI GATC 2 cut(s) 454, 566
BstMBI GATC 2 cut(s) 451, 563
BstMWI GCNNNNNNNGC 5 cut(s) 56, 436, 540, 582, 591
BstNI CCWGG 1 cut(s) 99
BstNSI RCATGY 2 cut(s) 74, 757
BstSCI CCNGG 1 cut(s) 97
BstUI CGCG 1 cut(s) 588
BstV1I GCAGC 2 cut(s) 76, 98
BstV2I GAAGAC 1 cut(s) 18
BstXI CCANNNNNNTGG 1 cut(s) 82
BstZ17I GTATAC 1 cut(s) 241
BsuI GTATCC 1 cut(s) 131
BsuRI GGCC 3 cut(s) 17, 222, 594
BtgI CCRYGG 1 cut(s) 586
BtgZI GCGATG 1 cut(s) 657
BtsCI GGATG 4 cut(s) 186, 231, 265, 426
BtsIMutI CAGTG 1 cut(s) 195
BveI ACCTGC 1 cut(s) 548
Cac8I GCNNGC 3 cut(s) 9, 19, 726
Cfr10I RCCGGY 1 cut(s) 724
Cfr42I CCGCGG 1 cut(s) 589
Csp6I GTAC 2 cut(s) 254, 495
CviAII CATG 5 cut(s) 71, 484, 529, 548, 754
CviQI GTAC 2 cut(s) 254, 495
DdeI CTNAG 3 cut(s) 12, 335, 680
DpnI GATC 2 cut(s) 453, 565
DpnII GATC 2 cut(s) 451, 563
Eam1104I CTCTTC 2 cut(s) 99, 420
EarI CTCTTC 2 cut(s) 99, 420
Eco130I CCWWGG 1 cut(s) 81
Eco147I AGGCCT 2 cut(s) 17, 222
Eco32I GATATC 1 cut(s) 64
EcoNI CCTNNNNNAGG 1 cut(s) 668
EcoRII CCWGG 1 cut(s) 97
EcoRV GATATC 1 cut(s) 64
EcoT14I CCWWGG 1 cut(s) 81
EcoT22I ATGCAT 1 cut(s) 485
ErhI CCWWGG 1 cut(s) 81
FaeI CATG 5 cut(s) 74, 487, 532, 551, 757
FatI CATG 5 cut(s) 70, 483, 528, 547, 753
FblI GTMKAC 1 cut(s) 240
Fnu4HI GCNGC 5 cut(s) 87, 90, 586, 589, 592
FokI GGATG 4 cut(s) 193, 218, 272, 433
Fsp4HI GCNGC 5 cut(s) 87, 90, 586, 589, 592
FspBI CTAG 2 cut(s) 455, 633
GluI GCNGC 5 cut(s) 87, 90, 586, 589, 592
GsuI CTGGAG 2 cut(s) 120, 595
HaeIII GGCC 3 cut(s) 17, 222, 594
HapII CCGG 1 cut(s) 725
Hin1II CATG 5 cut(s) 74, 487, 532, 551, 757
HinfI GANTC 2 cut(s) 38, 603
HpaII CCGG 1 cut(s) 725
HphI GGTGA 2 cut(s) 362, 529
Hpy166II GTNNAC 2 cut(s) 241, 521
Hpy188I TCNGA 2 cut(s) 445, 761
Hpy188III TCNNGA 3 cut(s) 53, 455, 612
Hpy8I GTNNAC 2 cut(s) 241, 521
HpyAV CCTTC 4 cut(s) 233, 279, 341, 664
HpyCH4III ACNGT 3 cut(s) 119, 494, 518
HpyCH4IV ACGT 1 cut(s) 685
HpyCH4V TGCA 8 cut(s) 21, 152, 195, 396, 483, 534, 560, 656
HpyF10VI GCNNNNNNNGC 5 cut(s) 56, 436, 540, 582, 591
HpyF3I CTNAG 3 cut(s) 12, 335, 680
HpySE526I ACGT 1 cut(s) 685
Hsp92II CATG 5 cut(s) 74, 487, 532, 551, 757
KroI GCCGGC 1 cut(s) 724
KroNI GCCGGC 1 cut(s) 726
KspI CCGCGG 1 cut(s) 589
Kzo9I GATC 2 cut(s) 451, 563
LguI GCTCTTC 2 cut(s) 99, 420
LmnI GCTCC 1 cut(s) 614
Lsp1109I GCAGC 2 cut(s) 76, 98
LweI GCATC 4 cut(s) 383, 543, 643, 645
MaeI CTAG 2 cut(s) 455, 633
MaeII ACGT 1 cut(s) 685
MaeIII GTNAC 1 cut(s) 658
MalI GATC 2 cut(s) 453, 565
MbiI CCGCTC 1 cut(s) 430
MboI GATC 2 cut(s) 451, 563
MboII GAAGA 5 cut(s) 18, 86, 151, 380, 437
MluCI AATT 3 cut(s) 211, 308, 353
MlyI GAGTC 1 cut(s) 612
MmeI TCCRAC 1 cut(s) 91
MnlI CCTC 3 cut(s) 289, 411, 426
Mph1103I ATGCAT 1 cut(s) 485
MroNI GCCGGC 1 cut(s) 724
MroXI GAANNNNTTC 1 cut(s) 268
MseI TTAA 2 cut(s) 302, 357
MspA1I CMGCKG 2 cut(s) 89, 588
MspI CCGG 1 cut(s) 725
MspR9I CCNGG 1 cut(s) 99
Mva1269I GAATGC 3 cut(s) 270, 439, 562
MvaI CCWGG 1 cut(s) 99
MvnI CGCG 1 cut(s) 588
MwoI GCNNNNNNNGC 5 cut(s) 56, 436, 540, 582, 591
NaeI GCCGGC 1 cut(s) 726
NdeII GATC 2 cut(s) 451, 563
NgoMIV GCCGGC 1 cut(s) 724
NlaIII CATG 5 cut(s) 74, 487, 532, 551, 757
NsiI ATGCAT 1 cut(s) 485
NspI RCATGY 2 cut(s) 74, 757
PaqCI CACCTGC 1 cut(s) 548
PceI AGGCCT 2 cut(s) 17, 222
PciSI GCTCTTC 2 cut(s) 99, 420
PctI GAATGC 3 cut(s) 270, 439, 562
PdiI GCCGGC 1 cut(s) 726
PdmI GAANNNNTTC 1 cut(s) 268
PfeI GAWTC 1 cut(s) 38
PfoI TCCNGGA 1 cut(s) 97
PkrI GCNGC 5 cut(s) 88, 91, 587, 590, 593
PleI GAGTC 1 cut(s) 611
PpsI GAGTC 1 cut(s) 611
Psp6I CCWGG 1 cut(s) 97
PspGI CCWGG 1 cut(s) 97
PvuII CAGCTG 1 cut(s) 89
RsaI GTAC 2 cut(s) 255, 496
RsaNI GTAC 2 cut(s) 254, 495
SacII CCGCGG 1 cut(s) 589
SapI GCTCTTC 2 cut(s) 99, 420
SaqAI TTAA 2 cut(s) 302, 357
SatI GCNGC 5 cut(s) 87, 90, 586, 589, 592
Sau3AI GATC 2 cut(s) 451, 563
SchI GAGTC 1 cut(s) 612
ScrFI CCNGG 1 cut(s) 99
SfaNI GCATC 4 cut(s) 383, 543, 643, 645
Sfr303I CCGCGG 1 cut(s) 589
SgrBI CCGCGG 1 cut(s) 589
Sse9I AATT 3 cut(s) 211, 308, 353
SseBI AGGCCT 2 cut(s) 17, 222
SsiI CCGC 4 cut(s) 430, 586, 588, 591
SspMI CTAG 2 cut(s) 455, 633
StuI AGGCCT 2 cut(s) 17, 222
StyD4I CCNGG 1 cut(s) 97
StyI CCWWGG 1 cut(s) 81
TaaI ACNGT 3 cut(s) 119, 494, 518
TaiI ACGT 1 cut(s) 688
TaqI TCGA 2 cut(s) 345, 566
TasI AATT 3 cut(s) 211, 308, 353
TatI WGTACW 2 cut(s) 253, 494
TauI GCSGC 3 cut(s) 588, 591, 594
TfiI GAWTC 1 cut(s) 38
Tru1I TTAA 2 cut(s) 302, 357
Tru9I TTAA 2 cut(s) 302, 357
TscAI CASTG 1 cut(s) 202
TseI GCWGC 2 cut(s) 86, 89
TspDTI ATGAA 1 cut(s) 483
TspRI CASTG 1 cut(s) 202
XagI CCTNNNNNAGG 1 cut(s) 668
XapI RAATTY 2 cut(s) 308, 353
XbaI TCTAGA 1 cut(s) 454
XceI RCATGY 2 cut(s) 74, 757
XmiI GTMKAC 1 cut(s) 240
XmnI GAANNNNTTC 1 cut(s) 268
XspI CTAG 2 cut(s) 455, 633
Zsp2I ATGCAT 1 cut(s) 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.