pycom13g09340

Prokaryotic RING finger family 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0009682
Physical Location & Seq
Forward (+)
6268770 .. 6270256
1487 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g09340.1

Sequence Viewer

Length: 762 bp
ATGTGGCAAGCTCAGGCCTGCAAGTCTTCTTATGGAGAATCCATCAAAGCTCTTGAAGCTGATATCCAACATGCCAATACCTTGGCAGCTGCTCTTCCCAGAGATTATGGTGGGAACTGTATCCAAATGAGATTGTCTTACAGCCCCTTTGCACCTTTCTTCCTGTATCTCATTGAATGGATGGATTGTAGCTGCACTGATATACTTCCCAATTATTTAGGCCTTCTCCACATCCTTGTACACAAGGTATATGTCGATGGGATGCCTTCATTGTCCTCCAAAGAACAGAAGGTCACTTTAAGGGAATTTTACGCTGTAATATACCCTTATCTTAGGCAACTTGAAGGTGAATTTAACGAACTGGAAGATAATAATAATAATAAGAGAAGCCGATGCACAGATGTTTTGGGCAGAAAGAGGATGGAAGACCGAAGGAAGCTTTCGGATAAAGATCTTGAGAGAGAAGATGAATGTGGGATATGCATGGAAAACTGTACAAAGATGGTGTTGCCCGACTGTGGGCATTCCATGTGCATCACTTGCTACCAGGATTGGAATGCAAGATCGAAATCCTGCCCTTTTTGCCGCGGCAGCCTAAAGAGAGTTAGTTCCAGAGATTTGTGGGTTCTCACTAGCATCAGCGATGTTATTGATGCAGTAACCCTTGCGCAGGAGAACCTAAGACGTTTCTATCTTTATATTGAAAATCTACCGGTAGTTGTACCGGCAACGCATGTTGTGGTGTATGATTACATGCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000122 GO:0000151 GO:0000209 GO:0000726 GO:0000781 GO:0001775 GO:0002200 GO:0002204 GO:0002208 GO:0002250 GO:0002252 GO:0002263 GO:0002285 GO:0002312 GO:0002366 GO:0002376 GO:0002377 GO:0002381 GO:0002440 GO:0002443 GO:0002449 GO:0002460 GO:0002520 GO:0002562 GO:0003006 GO:0003674 GO:0003682 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006281 GO:0006282 GO:0006302 GO:0006303 GO:0006310 GO:0006325 GO:0006338 GO:0006355 GO:0006357 GO:0006464 GO:0006508 GO:0006511 GO:0006725 GO:0006807 GO:0006950 GO:0006955 GO:0006974 GO:0006996 GO:0007275 GO:0007276 GO:0007281 GO:0007283 GO:0007286 GO:0008150 GO:0008152 GO:0008270 GO:0009056 GO:0009057 GO:0009314 GO:0009628 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009987 GO:0010212 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010629 GO:0016043 GO:0016064 GO:0016444 GO:0016445 GO:0016447 GO:0016567 GO:0016569 GO:0016570 GO:0016574 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019724 GO:0019787 GO:0019941 GO:0019953 GO:0022412 GO:0022414 GO:0030154 GO:0030163 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0032446 GO:0032501 GO:0032502 GO:0032504 GO:0032784 GO:0032785 GO:0032991 GO:0033522 GO:0033523 GO:0033554 GO:0034243 GO:0034244 GO:0034641 GO:0034728 GO:0035861 GO:0036211 GO:0042113 GO:0042393 GO:0042802 GO:0042803 GO:0043044 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0043486 GO:0043632 GO:0043933 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0044703 GO:0045190 GO:0045321 GO:0045739 GO:0045892 GO:0045934 GO:0045935 GO:0046483 GO:0046649 GO:0046872 GO:0046914 GO:0046983 GO:0048232 GO:0048468 GO:0048515 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048609 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051276 GO:0051603 GO:0051704 GO:0051716 GO:0060255 GO:0065007 GO:0070534 GO:0070535 GO:0070647 GO:0070936 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0080134 GO:0080135 GO:0090304 GO:0090734 GO:0098687 GO:0140096 GO:1901360 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:1990234 GO:2000112 GO:2000113 GO:2001020 GO:2001022 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

254

Amino Acids

29.21

Weight (kDa)

5.79

Isoelectric Point (pI)

68.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 155 - 201 1.1e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 156 - 196 2.6e-08 Ring finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 669
AccII CGCG 1 cut(s) 588
AciI CCGC 2 cut(s) 586, 588
AcsI RAATTY 2 cut(s) 305, 350
AfaI GTAC 3 cut(s) 240, 496, 723
AfiI CCNNNNNNNGG 3 cut(s) 518, 519, 670
AgeI ACCGGT 1 cut(s) 712
AgsI TTSAA 4 cut(s) 56, 176, 344, 704
AjnI CCWGG 1 cut(s) 546
AluBI AGCT 6 cut(s) 11, 50, 59, 89, 192, 439
AluI AGCT 6 cut(s) 11, 50, 59, 89, 192, 439
AoxI GGCC 2 cut(s) 15, 220
ApeKI GCWGC 4 cut(s) 86, 89, 192, 591
ApoI RAATTY 2 cut(s) 305, 350
AsiGI ACCGGT 1 cut(s) 712
AspLEI GCGC 1 cut(s) 670
AsuHPI GGTGA 1 cut(s) 359
BbsI GAAGAC 2 cut(s) 18, 432
BbvI GCAGC 4 cut(s) 76, 98, 179, 603
BccI CCATC 5 cut(s) 50, 175, 251, 415, 496
BciT130I CCWGG 1 cut(s) 548
BciVI GTATCC 1 cut(s) 131
BfaI CTAG 1 cut(s) 633
BfuI GTATCC 1 cut(s) 131
BglII AGATCT 1 cut(s) 451
BisI GCNGC 6 cut(s) 87, 90, 193, 586, 589, 592
BlsI GCNGC 6 cut(s) 88, 91, 194, 587, 590, 593
Bme1390I CCNGG 1 cut(s) 548
BmrFI CCNGG 1 cut(s) 548
BmsI GCATC 5 cut(s) 252, 383, 543, 643, 645
BpiI GAAGAC 2 cut(s) 18, 432
Bpu10I CCTNAGC 1 cut(s) 12
BpuEI CTTGAG 1 cut(s) 476
BsaBI GATNNNNATC 2 cut(s) 450, 568
BsaJI CCNNGG 2 cut(s) 81, 586
BsaWI WCCGGW 1 cut(s) 712
Bsc4I CCNNNNNNNGG 3 cut(s) 518, 519, 670
Bse118I RCCGGY 2 cut(s) 712, 724
Bse1I ACTGG 1 cut(s) 366
Bse8I GATNNNNATC 2 cut(s) 450, 568
BseBI CCWGG 1 cut(s) 548
BseDI CCNNGG 2 cut(s) 81, 586
BseGI GGATG 4 cut(s) 186, 231, 267, 426
BseJI GATNNNNATC 2 cut(s) 450, 568
BseLI CCNNNNNNNGG 3 cut(s) 518, 519, 670
BseMII CTCAG 1 cut(s) 26
BseNI ACTGG 1 cut(s) 366
BseXI GCAGC 4 cut(s) 76, 98, 179, 603
BsgI GTGCAG 1 cut(s) 178
Bsh1236I CGCG 1 cut(s) 588
BshFI GGCC 2 cut(s) 17, 222
BshTI ACCGGT 1 cut(s) 712
BsiSI CCGG 2 cut(s) 713, 725
BslI CCNNNNNNNGG 3 cut(s) 518, 519, 670
BsmI GAATGC 2 cut(s) 523, 562
BsnI GGCC 2 cut(s) 17, 222
Bsp1407I TGTACA 2 cut(s) 238, 494
Bsp143I GATC 2 cut(s) 451, 563
BspACI CCGC 2 cut(s) 586, 588
BspANI GGCC 2 cut(s) 17, 222
BspCNI CTCAG 1 cut(s) 25
BspFNI CGCG 1 cut(s) 588
BspQI GCTCTTC 1 cut(s) 99
BsrFI RCCGGY 2 cut(s) 712, 724
BsrGI TGTACA 2 cut(s) 238, 494
BsrI ACTGG 1 cut(s) 366
BssAI RCCGGY 2 cut(s) 712, 724
BssECI CCNNGG 2 cut(s) 81, 586
BssMI GATC 2 cut(s) 451, 563
BssT1I CCWWGG 1 cut(s) 81
Bst2UI CCWGG 1 cut(s) 548
Bst4CI ACNGT 3 cut(s) 119, 494, 518
Bst6I CTCTTC 1 cut(s) 99
BstAPI GCANNNNNTGC 1 cut(s) 540
BstAUI TGTACA 2 cut(s) 238, 494
BstC8I GCNNGC 2 cut(s) 9, 19
BstDEI CTNAG 3 cut(s) 12, 332, 680
BstDSI CCRYGG 1 cut(s) 586
BstENI CCTNNNNNAGG 1 cut(s) 668
BstF5I GGATG 4 cut(s) 186, 231, 267, 426
BstFNI CGCG 1 cut(s) 588
BstHHI GCGC 1 cut(s) 670
BstKTI GATC 2 cut(s) 454, 566
BstMBI GATC 2 cut(s) 451, 563
BstMWI GCNNNNNNNGC 4 cut(s) 56, 540, 582, 591
BstNI CCWGG 1 cut(s) 548
BstNSI RCATGY 3 cut(s) 74, 737, 757
BstSCI CCNGG 1 cut(s) 546
BstUI CGCG 1 cut(s) 588
BstV1I GCAGC 4 cut(s) 76, 98, 179, 603
BstV2I GAAGAC 2 cut(s) 18, 432
BstX2I RGATCY 1 cut(s) 451
BstXI CCANNNNNNTGG 1 cut(s) 82
BstYI RGATCY 1 cut(s) 451
BsuI GTATCC 1 cut(s) 131
BsuRI GGCC 2 cut(s) 17, 222
BtgI CCRYGG 1 cut(s) 586
BtgZI GCGATG 1 cut(s) 657
BtsCI GGATG 4 cut(s) 186, 231, 267, 426
BtsIMutI CAGTG 1 cut(s) 195
Cac8I GCNNGC 2 cut(s) 9, 19
CfoI GCGC 1 cut(s) 670
Cfr10I RCCGGY 2 cut(s) 712, 724
Cfr42I CCGCGG 1 cut(s) 589
Csp6I GTAC 3 cut(s) 239, 495, 722
CspAI ACCGGT 1 cut(s) 712
CviAII CATG 5 cut(s) 71, 484, 529, 734, 754
CviQI GTAC 3 cut(s) 239, 495, 722
DdeI CTNAG 3 cut(s) 12, 332, 680
DpnI GATC 2 cut(s) 453, 565
DpnII GATC 2 cut(s) 451, 563
Eam1104I CTCTTC 1 cut(s) 99
EarI CTCTTC 1 cut(s) 99
Eco130I CCWWGG 1 cut(s) 81
Eco147I AGGCCT 2 cut(s) 17, 222
Eco32I GATATC 1 cut(s) 64
EcoNI CCTNNNNNAGG 1 cut(s) 668
EcoRII CCWGG 1 cut(s) 546
EcoRV GATATC 1 cut(s) 64
EcoT14I CCWWGG 1 cut(s) 81
EcoT22I ATGCAT 1 cut(s) 485
ErhI CCWWGG 1 cut(s) 81
FaeI CATG 5 cut(s) 74, 487, 532, 737, 757
FatI CATG 5 cut(s) 70, 483, 528, 733, 753
Fnu4HI GCNGC 6 cut(s) 87, 90, 193, 586, 589, 592
FokI GGATG 4 cut(s) 193, 218, 274, 433
Fsp4HI GCNGC 6 cut(s) 87, 90, 193, 586, 589, 592
FspBI CTAG 1 cut(s) 633
FspI TGCGCA 1 cut(s) 669
GlaI GCGC 1 cut(s) 669
GluI GCNGC 6 cut(s) 87, 90, 193, 586, 589, 592
HaeIII GGCC 2 cut(s) 17, 222
HapII CCGG 2 cut(s) 713, 725
HhaI GCGC 1 cut(s) 670
Hin1II CATG 5 cut(s) 74, 487, 532, 737, 757
Hin6I GCGC 1 cut(s) 668
HinP1I GCGC 1 cut(s) 668
HindIII AAGCTT 1 cut(s) 437
HinfI GANTC 1 cut(s) 38
HpaII CCGG 2 cut(s) 713, 725
HphI GGTGA 1 cut(s) 359
Hpy166II GTNNAC 1 cut(s) 241
Hpy188I TCNGA 2 cut(s) 445, 761
Hpy188III TCNNGA 3 cut(s) 53, 455, 612
Hpy8I GTNNAC 1 cut(s) 241
HpyAV CCTTC 5 cut(s) 233, 276, 283, 338, 426
HpyCH4III ACNGT 3 cut(s) 119, 494, 518
HpyCH4IV ACGT 1 cut(s) 685
HpyCH4V TGCA 8 cut(s) 21, 152, 195, 396, 483, 534, 560, 656
HpyF10VI GCNNNNNNNGC 4 cut(s) 56, 540, 582, 591
HpyF3I CTNAG 3 cut(s) 12, 332, 680
HpySE526I ACGT 1 cut(s) 685
Hsp92II CATG 5 cut(s) 74, 487, 532, 737, 757
HspAI GCGC 1 cut(s) 668
KspI CCGCGG 1 cut(s) 589
Kzo9I GATC 2 cut(s) 451, 563
LguI GCTCTTC 1 cut(s) 99
Lsp1109I GCAGC 4 cut(s) 76, 98, 179, 603
LweI GCATC 5 cut(s) 252, 383, 543, 643, 645
MaeI CTAG 1 cut(s) 633
MaeII ACGT 1 cut(s) 685
MaeIII GTNAC 2 cut(s) 292, 658
MalI GATC 2 cut(s) 453, 565
MboI GATC 2 cut(s) 451, 563
MboII GAAGA 6 cut(s) 18, 86, 151, 377, 437, 476
MflI RGATCY 1 cut(s) 451
MluCI AATT 3 cut(s) 211, 305, 350
MmeI TCCRAC 1 cut(s) 91
MnlI CCTC 2 cut(s) 286, 411
Mph1103I ATGCAT 1 cut(s) 485
MseI TTAA 2 cut(s) 299, 354
MspA1I CMGCKG 2 cut(s) 89, 588
MspI CCGG 2 cut(s) 713, 725
MspR9I CCNGG 1 cut(s) 548
Mva1269I GAATGC 2 cut(s) 523, 562
MvaI CCWGG 1 cut(s) 548
MvnI CGCG 1 cut(s) 588
MwoI GCNNNNNNNGC 4 cut(s) 56, 540, 582, 591
NdeII GATC 2 cut(s) 451, 563
NlaIII CATG 5 cut(s) 74, 487, 532, 737, 757
NmuCI GTSAC 1 cut(s) 292
NsbI TGCGCA 1 cut(s) 669
NsiI ATGCAT 1 cut(s) 485
NspI RCATGY 3 cut(s) 74, 737, 757
PceI AGGCCT 2 cut(s) 17, 222
PciSI GCTCTTC 1 cut(s) 99
PctI GAATGC 2 cut(s) 523, 562
PfeI GAWTC 1 cut(s) 38
PinAI ACCGGT 1 cut(s) 712
PkrI GCNGC 6 cut(s) 88, 91, 194, 587, 590, 593
Psp6I CCWGG 1 cut(s) 546
PspGI CCWGG 1 cut(s) 546
PsuI RGATCY 1 cut(s) 451
PvuII CAGCTG 1 cut(s) 89
RsaI GTAC 3 cut(s) 240, 496, 723
RsaNI GTAC 3 cut(s) 239, 495, 722
SacII CCGCGG 1 cut(s) 589
SapI GCTCTTC 1 cut(s) 99
SaqAI TTAA 2 cut(s) 299, 354
SatI GCNGC 6 cut(s) 87, 90, 193, 586, 589, 592
Sau3AI GATC 2 cut(s) 451, 563
ScrFI CCNGG 1 cut(s) 548
SfaNI GCATC 5 cut(s) 252, 383, 543, 643, 645
Sfr303I CCGCGG 1 cut(s) 589
SgrBI CCGCGG 1 cut(s) 589
SmlI CTYRAG 1 cut(s) 455
SmoI CTYRAG 1 cut(s) 455
Sse9I AATT 3 cut(s) 211, 305, 350
SseBI AGGCCT 2 cut(s) 17, 222
SsiI CCGC 2 cut(s) 586, 588
SspMI CTAG 1 cut(s) 633
StuI AGGCCT 2 cut(s) 17, 222
StyD4I CCNGG 1 cut(s) 546
StyI CCWWGG 1 cut(s) 81
TaaI ACNGT 3 cut(s) 119, 494, 518
TaiI ACGT 1 cut(s) 688
TaqI TCGA 2 cut(s) 255, 566
TaqII GACCGA 1 cut(s) 444
TasI AATT 3 cut(s) 211, 305, 350
TatI WGTACW 2 cut(s) 238, 494
TauI GCSGC 2 cut(s) 588, 591
TfiI GAWTC 1 cut(s) 38
Tru1I TTAA 2 cut(s) 299, 354
Tru9I TTAA 2 cut(s) 299, 354
TscAI CASTG 1 cut(s) 202
TseFI GTSAC 1 cut(s) 292
TseI GCWGC 4 cut(s) 86, 89, 192, 591
Tsp45I GTSAC 1 cut(s) 292
TspDTI ATGAA 2 cut(s) 258, 483
TspRI CASTG 1 cut(s) 202
XagI CCTNNNNNAGG 1 cut(s) 668
XapI RAATTY 2 cut(s) 305, 350
XceI RCATGY 3 cut(s) 74, 737, 757
XspI CTAG 1 cut(s) 633
Zsp2I ATGCAT 1 cut(s) 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.