RchiOBHm_Chr4g0431951

Prokaryotic RING finger family 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0009682
Physical Location & Seq
Reverse (-)
56071576 .. 56074094
2519 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40059

Sequence Viewer

Length: 735 bp
ATGTGGCAAGATCAGCCCAGAAAGTCTTGTTTCGGAGAATCTATCAAGGCTCTTGAGGCTGATATACAACATGCCAATAGCTTGGCAGCTTCTCTTCCCAGAGATTATGGTGGGAATTCTATCCAAATGAGATTGTCTTACAGCCCCTTTGCCCCTCTTTTCCTTTATTTCATTGAATGGATGGATTATAGTTGCACTGATATACTTCCCAATTACTTGGGCCTCCTCCACATAATTGTATACAAGGTATATGTTGATGGGGTTCCTTCAATATCCTCCAAAGAACAAAAGGTCACTATAAGGGAATTTTATGCTGTAATATACCCTTATCTTAGACAACTTGAAGGCGAGTTTAACGAACTGGAAGATAATAAGAAGAGAAGCCAAAAGAGGATGGAAGACAGGAAAAACCTCTCTGATCAGGATCTAGAAAGAGATGATGAATGTGGTATATGTATGGAAAACTATGCAAAGATGGTGTTGCCGAATTGTGGGCATTCTATGTGCATCACCTGCTTCCATGATTGGAATGCGCGATCACAGTCCTGCCCTTTTTGCCGTGGCAACCTCAAGAGAGTTAGCTCTACAGATTTGTGGGTACTCACTAGAAACAGCGACGTGATTGATGCCGTAACCCTTGCAAAAGAGAACCTTAGACGCTTCTATCTTTATGTTGAAAATATTCCTGTTGTTATGCCAGCAACACATGTTGTGGTATATGATTACATGCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000122 GO:0000151 GO:0000209 GO:0000726 GO:0000781 GO:0001775 GO:0002200 GO:0002204 GO:0002208 GO:0002250 GO:0002252 GO:0002263 GO:0002285 GO:0002312 GO:0002366 GO:0002376 GO:0002377 GO:0002381 GO:0002440 GO:0002443 GO:0002449 GO:0002460 GO:0002520 GO:0002562 GO:0003006 GO:0003674 GO:0003682 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006281 GO:0006282 GO:0006302 GO:0006303 GO:0006310 GO:0006325 GO:0006338 GO:0006355 GO:0006357 GO:0006464 GO:0006508 GO:0006511 GO:0006725 GO:0006807 GO:0006950 GO:0006955 GO:0006974 GO:0006996 GO:0007275 GO:0007276 GO:0007281 GO:0007283 GO:0007286 GO:0008150 GO:0008152 GO:0008270 GO:0009056 GO:0009057 GO:0009314 GO:0009628 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009987 GO:0010212 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010629 GO:0016043 GO:0016064 GO:0016444 GO:0016445 GO:0016447 GO:0016567 GO:0016569 GO:0016570 GO:0016574 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019724 GO:0019787 GO:0019941 GO:0019953 GO:0022412 GO:0022414 GO:0030154 GO:0030163 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0032446 GO:0032501 GO:0032502 GO:0032504 GO:0032784 GO:0032785 GO:0032991 GO:0033522 GO:0033523 GO:0033554 GO:0034243 GO:0034244 GO:0034641 GO:0034728 GO:0035861 GO:0036211 GO:0042113 GO:0042393 GO:0042802 GO:0042803 GO:0043044 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0043486 GO:0043632 GO:0043933 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0044703 GO:0045190 GO:0045321 GO:0045739 GO:0045892 GO:0045934 GO:0045935 GO:0046483 GO:0046649 GO:0046872 GO:0046914 GO:0046983 GO:0048232 GO:0048468 GO:0048515 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048609 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051276 GO:0051603 GO:0051704 GO:0051716 GO:0060255 GO:0065007 GO:0070534 GO:0070535 GO:0070647 GO:0070936 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0080134 GO:0080135 GO:0090304 GO:0090734 GO:0098687 GO:0140096 GO:1901360 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:1990234 GO:2000112 GO:2000113 GO:2001020 GO:2001022 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

244

Amino Acids

28.41

Weight (kDa)

5.59

Isoelectric Point (pI)

64.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 146 - 192 9.6e-11 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 147 - 187 3.8e-09 Ring finger domain
zf-RING_5 PF14634 148 - 187 1.3e-06 zinc-RING finger domain
zf-C3HC4_2 PF13923 148 - 186 1.9e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 149 - 186 1.1e-06 Zinc finger, C3HC4 type (RING finger)
Prok-RING_4 PF14447 149 - 191 1.1e-06 Prokaryotic RING finger family 4
zf-RING_UBOX PF13445 149 - 184 7.3e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 521
Acc36I ACCTGC 1 cut(s) 521
AccI GTMKAC 1 cut(s) 240
AccII CGCG 1 cut(s) 535
AclWI GGATC 1 cut(s) 432
AcsI RAATTY 2 cut(s) 115, 305
AfaI GTAC 1 cut(s) 600
AfiI CCNNNNNNNGG 1 cut(s) 491
AflIII ACRYGT 1 cut(s) 706
AgsI TTSAA 4 cut(s) 176, 270, 344, 677
AjiI CACGTC 1 cut(s) 619
AluBI AGCT 3 cut(s) 81, 89, 582
AluI AGCT 3 cut(s) 81, 89, 582
AlwI GGATC 1 cut(s) 432
AoxI GGCC 1 cut(s) 220
ApeKI GCWGC 1 cut(s) 86
ApoI RAATTY 2 cut(s) 115, 305
Asp700I GAANNNNTTC 1 cut(s) 681
AspLEI GCGC 1 cut(s) 535
AspS9I GGNCC 1 cut(s) 220
AsuHPI GGTGA 1 cut(s) 502
BbsI GAAGAC 1 cut(s) 405
BbvI GCAGC 1 cut(s) 98
BccI CCATC 4 cut(s) 175, 251, 388, 469
BceAI ACGGC 2 cut(s) 543, 614
BclI TGATCA 1 cut(s) 418
BfaI CTAG 2 cut(s) 428, 606
BfmI CTRYAG 1 cut(s) 585
BfuAI ACCTGC 1 cut(s) 521
BisI GCNGC 1 cut(s) 87
BlsI GCNGC 1 cut(s) 88
BmgBI CACGTC 1 cut(s) 619
BmgT120I GGNCC 1 cut(s) 220
BmiI GGNNCC 1 cut(s) 264
BmsI GCATC 2 cut(s) 516, 616
BpiI GAAGAC 1 cut(s) 405
BpuEI CTTGAG 2 cut(s) 74, 554
BsaBI GATNNNNATC 1 cut(s) 423
BsaJI CCNNGG 1 cut(s) 559
Bsc4I CCNNNNNNNGG 1 cut(s) 491
Bse1I ACTGG 1 cut(s) 366
Bse8I GATNNNNATC 1 cut(s) 423
BseDI CCNNGG 1 cut(s) 559
BseGI GGATG 2 cut(s) 186, 399
BseJI GATNNNNATC 1 cut(s) 423
BseLI CCNNNNNNNGG 1 cut(s) 491
BseNI ACTGG 1 cut(s) 366
BseRI GAGGAG 1 cut(s) 215
BseXI GCAGC 1 cut(s) 98
Bsh1236I CGCG 1 cut(s) 535
BshFI GGCC 1 cut(s) 222
BslI CCNNNNNNNGG 1 cut(s) 491
BsmI GAATGC 2 cut(s) 496, 535
BsnI GGCC 1 cut(s) 222
Bsp143I GATC 4 cut(s) 10, 418, 424, 536
BspANI GGCC 1 cut(s) 222
BspFNI CGCG 1 cut(s) 535
BspLI GGNNCC 1 cut(s) 264
BspMI ACCTGC 1 cut(s) 521
BspPI GGATC 1 cut(s) 432
BsrI ACTGG 1 cut(s) 366
BssECI CCNNGG 1 cut(s) 559
BssMI GATC 4 cut(s) 10, 418, 424, 536
BssNAI GTATAC 1 cut(s) 241
Bst1107I GTATAC 1 cut(s) 241
Bst4CI ACNGT 1 cut(s) 543
Bst6I CTCTTC 2 cut(s) 99, 371
BstAPI GCANNNNNTGC 1 cut(s) 513
BstC8I GCNNGC 1 cut(s) 699
BstDEI CTNAG 2 cut(s) 332, 653
BstDSI CCRYGG 1 cut(s) 559
BstF5I GGATG 2 cut(s) 186, 399
BstFNI CGCG 1 cut(s) 535
BstHHI GCGC 1 cut(s) 535
BstKTI GATC 4 cut(s) 13, 421, 427, 539
BstMBI GATC 4 cut(s) 10, 418, 424, 536
BstMWI GCNNNNNNNGC 4 cut(s) 13, 56, 513, 555
BstNSI RCATGY 3 cut(s) 74, 710, 730
BstSFI CTRYAG 1 cut(s) 585
BstUI CGCG 1 cut(s) 535
BstV1I GCAGC 1 cut(s) 98
BstV2I GAAGAC 1 cut(s) 405
BstX2I RGATCY 1 cut(s) 424
BstXI CCANNNNNNTGG 2 cut(s) 82, 217
BstYI RGATCY 1 cut(s) 424
BstZ17I GTATAC 1 cut(s) 241
BsuRI GGCC 1 cut(s) 222
BtgI CCRYGG 1 cut(s) 559
BtrI CACGTC 1 cut(s) 619
BtsCI GGATG 2 cut(s) 186, 399
BtsIMutI CAGTG 1 cut(s) 195
BveI ACCTGC 1 cut(s) 521
Cac8I GCNNGC 1 cut(s) 699
CfoI GCGC 1 cut(s) 535
Cfr13I GGNCC 1 cut(s) 220
CseI GACGC 1 cut(s) 666
Csp6I GTAC 1 cut(s) 599
CviAII CATG 4 cut(s) 71, 521, 707, 727
CviJI RGCY 9 cut(s) 16, 50, 59, 81, 89, 144, 222, 384, 582
CviKI_1 RGCY 9 cut(s) 16, 50, 59, 81, 89, 144, 222, 384, 582
CviQI GTAC 1 cut(s) 599
DdeI CTNAG 2 cut(s) 332, 653
DpnI GATC 4 cut(s) 12, 420, 426, 538
DpnII GATC 4 cut(s) 10, 418, 424, 536
Eam1104I CTCTTC 2 cut(s) 99, 371
EarI CTCTTC 2 cut(s) 99, 371
EcoRI GAATTC 1 cut(s) 115
FaeI CATG 4 cut(s) 74, 524, 710, 730
FalI AAGNNNNNCTT 2 cut(s) 636, 668
FatI CATG 4 cut(s) 70, 520, 706, 726
FbaI TGATCA 1 cut(s) 418
FblI GTMKAC 1 cut(s) 240
Fnu4HI GCNGC 1 cut(s) 87
FokI GGATG 2 cut(s) 193, 406
Fsp4HI GCNGC 1 cut(s) 87
FspBI CTAG 2 cut(s) 428, 606
GlaI GCGC 1 cut(s) 534
GluI GCNGC 1 cut(s) 87
HaeIII GGCC 1 cut(s) 222
HgaI GACGC 1 cut(s) 666
HhaI GCGC 1 cut(s) 535
Hin1II CATG 4 cut(s) 74, 524, 710, 730
Hin6I GCGC 1 cut(s) 533
HinP1I GCGC 1 cut(s) 533
HinfI GANTC 1 cut(s) 38
HphI GGTGA 1 cut(s) 502
Hpy166II GTNNAC 1 cut(s) 241
Hpy188I TCNGA 3 cut(s) 35, 418, 734
Hpy188III TCNNGA 4 cut(s) 53, 422, 428, 571
Hpy8I GTNNAC 1 cut(s) 241
Hpy99I CGWCG 1 cut(s) 620
HpyAV CCTTC 2 cut(s) 276, 338
HpyCH4III ACNGT 1 cut(s) 543
HpyCH4IV ACGT 1 cut(s) 618
HpyCH4V TGCA 4 cut(s) 195, 470, 507, 641
HpyF10VI GCNNNNNNNGC 4 cut(s) 13, 56, 513, 555
HpyF3I CTNAG 2 cut(s) 332, 653
HpySE526I ACGT 1 cut(s) 618
Hsp92II CATG 4 cut(s) 74, 524, 710, 730
HspAI GCGC 1 cut(s) 533
Ksp22I TGATCA 1 cut(s) 418
Kzo9I GATC 4 cut(s) 10, 418, 424, 536
LpnPI CCDG 9 cut(s) 31, 112, 347, 388, 407, 526, 559, 699, 711
Lsp1109I GCAGC 1 cut(s) 98
LweI GCATC 2 cut(s) 516, 616
MaeI CTAG 2 cut(s) 428, 606
MaeII ACGT 1 cut(s) 618
MaeIII GTNAC 2 cut(s) 292, 631
MalI GATC 4 cut(s) 12, 420, 426, 538
MboI GATC 4 cut(s) 10, 418, 424, 536
MboII GAAGA 4 cut(s) 86, 377, 388, 410
MflI RGATCY 1 cut(s) 424
MluCI AATT 5 cut(s) 115, 211, 234, 305, 487
MnlI CCTC 8 cut(s) 49, 165, 233, 236, 286, 384, 422, 578
MroXI GAANNNNTTC 1 cut(s) 681
MseI TTAA 1 cut(s) 354
Mva1269I GAATGC 2 cut(s) 496, 535
MvnI CGCG 1 cut(s) 535
MwoI GCNNNNNNNGC 4 cut(s) 13, 56, 513, 555
NdeII GATC 4 cut(s) 10, 418, 424, 536
NlaIII CATG 4 cut(s) 74, 524, 710, 730
NlaIV GGNNCC 1 cut(s) 264
NmuCI GTSAC 1 cut(s) 292
NspI RCATGY 3 cut(s) 74, 710, 730
PaqCI CACCTGC 1 cut(s) 521
PciI ACATGT 1 cut(s) 706
PctI GAATGC 2 cut(s) 496, 535
PdmI GAANNNNTTC 1 cut(s) 681
PfeI GAWTC 1 cut(s) 38
PkrI GCNGC 1 cut(s) 88
PscI ACATGT 1 cut(s) 706
PspN4I GGNNCC 1 cut(s) 264
PspPI GGNCC 1 cut(s) 220
PsuI RGATCY 1 cut(s) 424
RsaI GTAC 1 cut(s) 600
RsaNI GTAC 1 cut(s) 599
SaqAI TTAA 1 cut(s) 354
SatI GCNGC 1 cut(s) 87
Sau3AI GATC 4 cut(s) 10, 418, 424, 536
Sau96I GGNCC 1 cut(s) 220
SfaNI GCATC 2 cut(s) 516, 616
SfcI CTRYAG 1 cut(s) 585
SmlI CTYRAG 2 cut(s) 53, 569
SmoI CTYRAG 2 cut(s) 53, 569
Sse9I AATT 5 cut(s) 115, 211, 234, 305, 487
SspI AATATT 1 cut(s) 682
SspMI CTAG 2 cut(s) 428, 606
TaaI ACNGT 1 cut(s) 543
TaiI ACGT 1 cut(s) 621
TasI AATT 5 cut(s) 115, 211, 234, 305, 487
TfiI GAWTC 1 cut(s) 38
Tru1I TTAA 1 cut(s) 354
Tru9I TTAA 1 cut(s) 354
TscAI CASTG 1 cut(s) 202
TseFI GTSAC 1 cut(s) 292
TseI GCWGC 1 cut(s) 86
Tsp45I GTSAC 1 cut(s) 292
TspDTI ATGAA 2 cut(s) 160, 456
TspRI CASTG 1 cut(s) 202
XapI RAATTY 2 cut(s) 115, 305
XbaI TCTAGA 1 cut(s) 427
XceI RCATGY 3 cut(s) 74, 710, 730
XmiI GTMKAC 1 cut(s) 240
XmnI GAANNNNTTC 1 cut(s) 681
XspI CTAG 2 cut(s) 428, 606
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.