MD16G1108200.v1.1

Prokaryotic RING finger family 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0009682
Physical Location & Seq
Forward (+)
7564644 .. 7566732
2089 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1108200.v1.1.491

Sequence Viewer

Length: 750 bp
ATGTGGCAAGCTCAGGCCTGCAAGTCTTCTTATGGAGAATCCATCAAAGCTCTTGAAGCTGATATCCAACATGCCAATACCTTGGCAGCTGCTCTTCCTGGAGATTATGATGGGAACTGTATCCAAATGAGATTGTCTTATAGCCCCTTTGCACCTTTCTTCCTGTATCTCATTGAATGGATGGATTGTTGTTGCACTGATATACTTCCCAATTATTTAGGCCTTCACCACGTCCTTGTATACAAGGATGGAATGCCTTCGTTGTCCTCCAAAGAACAGGTCGCCACTTTAAGGGAATTTTATGCTGTAATATACCCTTATCTTAGACAACTCGAAGGTGAATTTAACGAACTGGAAGATAATAATAATAAGAGAAGCCGATGCACAGATGTTTTGGGCAGAAAGAGGATGGAAGACCGGAGGAAGCATTCGGATAAAGATCTAGAGAGAGATGATGAATGTGGGATATGCATGGAAAACTGTACAAAAATGGTGTTGCCCAACTGTGGACATTCCATGTGCATCACCTGCTTCCACGATTGGAATGCAAGATCGAAATCCTGCCCTTTTTGCCGCGGCAGCCTAAAGAGAGTTAGCTCCAGAGATTTGTGGGTTCTCACTAGCATCGGCGATGTTATTGATGCAGTAACCCTTGCGAAGGAGAACCTAAGACGTTTCTATCTTTATATTGAAAATCTACCTGTTGTTGTGCCGGCAACACCTATTGTGGTATATGATTACATGCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000122 GO:0000151 GO:0000209 GO:0000726 GO:0000781 GO:0001775 GO:0002200 GO:0002204 GO:0002208 GO:0002250 GO:0002252 GO:0002263 GO:0002285 GO:0002312 GO:0002366 GO:0002376 GO:0002377 GO:0002381 GO:0002440 GO:0002443 GO:0002449 GO:0002460 GO:0002520 GO:0002562 GO:0003006 GO:0003674 GO:0003682 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006281 GO:0006282 GO:0006302 GO:0006303 GO:0006310 GO:0006325 GO:0006338 GO:0006355 GO:0006357 GO:0006464 GO:0006508 GO:0006511 GO:0006725 GO:0006807 GO:0006950 GO:0006955 GO:0006974 GO:0006996 GO:0007275 GO:0007276 GO:0007281 GO:0007283 GO:0007286 GO:0008150 GO:0008152 GO:0008270 GO:0009056 GO:0009057 GO:0009314 GO:0009628 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009987 GO:0010212 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010629 GO:0016043 GO:0016064 GO:0016444 GO:0016445 GO:0016447 GO:0016567 GO:0016569 GO:0016570 GO:0016574 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019724 GO:0019787 GO:0019941 GO:0019953 GO:0022412 GO:0022414 GO:0030154 GO:0030163 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0032446 GO:0032501 GO:0032502 GO:0032504 GO:0032784 GO:0032785 GO:0032991 GO:0033522 GO:0033523 GO:0033554 GO:0034243 GO:0034244 GO:0034641 GO:0034728 GO:0035861 GO:0036211 GO:0042113 GO:0042393 GO:0042802 GO:0042803 GO:0043044 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0043486 GO:0043632 GO:0043933 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0044703 GO:0045190 GO:0045321 GO:0045739 GO:0045892 GO:0045934 GO:0045935 GO:0046483 GO:0046649 GO:0046872 GO:0046914 GO:0046983 GO:0048232 GO:0048468 GO:0048515 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048609 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051276 GO:0051603 GO:0051704 GO:0051716 GO:0060255 GO:0065007 GO:0070534 GO:0070535 GO:0070647 GO:0070936 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0080134 GO:0080135 GO:0090304 GO:0090734 GO:0098687 GO:0140096 GO:1901360 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:1990234 GO:2000112 GO:2000113 GO:2001020 GO:2001022 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

250

Amino Acids

28.63

Weight (kDa)

5.71

Isoelectric Point (pI)

65.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 151 - 197 2.4e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 152 - 192 8.6e-08 Ring finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 536
Acc36I ACCTGC 1 cut(s) 536
AccI GTMKAC 1 cut(s) 240
AccII CGCG 1 cut(s) 576
AciI CCGC 2 cut(s) 574, 576
AcsI RAATTY 2 cut(s) 296, 341
AfaI GTAC 1 cut(s) 484
AfiI CCNNNNNNNGG 3 cut(s) 291, 506, 658
AgsI TTSAA 3 cut(s) 56, 176, 692
AjiI CACGTC 1 cut(s) 232
AjnI CCWGG 1 cut(s) 97
AluBI AGCT 5 cut(s) 11, 50, 59, 89, 597
AluI AGCT 5 cut(s) 11, 50, 59, 89, 597
AoxI GGCC 2 cut(s) 15, 220
ApeKI GCWGC 3 cut(s) 86, 89, 579
ApoI RAATTY 2 cut(s) 296, 341
ArsI GACNNNNNNTTYG 2 cut(s) 264, 296
Asp700I GAANNNNTTC 1 cut(s) 256
AsuHPI GGTGA 3 cut(s) 218, 350, 517
BbsI GAAGAC 2 cut(s) 18, 420
BbvI GCAGC 3 cut(s) 76, 98, 591
BccI CCATC 5 cut(s) 50, 104, 175, 242, 403
BcgI CGANNNNNNTGC 2 cut(s) 527, 561
BciT130I CCWGG 1 cut(s) 99
BciVI GTATCC 1 cut(s) 131
BfaI CTAG 2 cut(s) 443, 621
BfuAI ACCTGC 1 cut(s) 536
BfuI GTATCC 1 cut(s) 131
BglII AGATCT 1 cut(s) 439
BisI GCNGC 5 cut(s) 87, 90, 574, 577, 580
BlsI GCNGC 5 cut(s) 88, 91, 575, 578, 581
Bme1390I CCNGG 1 cut(s) 99
BmgBI CACGTC 1 cut(s) 232
BmrFI CCNGG 1 cut(s) 99
BmsI GCATC 4 cut(s) 371, 531, 631, 633
BpiI GAAGAC 2 cut(s) 18, 420
BpmI CTGGAG 2 cut(s) 120, 583
Bpu10I CCTNAGC 1 cut(s) 12
BsaBI GATNNNNATC 2 cut(s) 438, 556
BsaJI CCNNGG 2 cut(s) 81, 574
BsaWI WCCGGW 1 cut(s) 417
Bsc4I CCNNNNNNNGG 3 cut(s) 291, 506, 658
Bse118I RCCGGY 1 cut(s) 712
Bse1I ACTGG 1 cut(s) 357
Bse8I GATNNNNATC 2 cut(s) 438, 556
BseBI CCWGG 1 cut(s) 99
BseDI CCNNGG 2 cut(s) 81, 574
BseGI GGATG 3 cut(s) 186, 253, 414
BseJI GATNNNNATC 2 cut(s) 438, 556
BseLI CCNNNNNNNGG 3 cut(s) 291, 506, 658
BseMII CTCAG 1 cut(s) 26
BseNI ACTGG 1 cut(s) 357
BseXI GCAGC 3 cut(s) 76, 98, 591
Bsh1236I CGCG 1 cut(s) 576
BshFI GGCC 2 cut(s) 17, 222
BsiSI CCGG 2 cut(s) 418, 713
BslI CCNNNNNNNGG 3 cut(s) 291, 506, 658
BsmI GAATGC 3 cut(s) 258, 427, 550
BsnI GGCC 2 cut(s) 17, 222
Bsp1407I TGTACA 1 cut(s) 482
Bsp143I GATC 2 cut(s) 439, 551
BspACI CCGC 2 cut(s) 574, 576
BspANI GGCC 2 cut(s) 17, 222
BspCNI CTCAG 1 cut(s) 25
BspFNI CGCG 1 cut(s) 576
BspMI ACCTGC 1 cut(s) 536
BspQI GCTCTTC 1 cut(s) 99
BsrFI RCCGGY 1 cut(s) 712
BsrGI TGTACA 1 cut(s) 482
BsrI ACTGG 1 cut(s) 357
BssAI RCCGGY 1 cut(s) 712
BssECI CCNNGG 2 cut(s) 81, 574
BssMI GATC 2 cut(s) 439, 551
BssNAI GTATAC 1 cut(s) 241
BssT1I CCWWGG 1 cut(s) 81
Bst1107I GTATAC 1 cut(s) 241
Bst2UI CCWGG 1 cut(s) 99
Bst4CI ACNGT 3 cut(s) 119, 482, 506
Bst6I CTCTTC 1 cut(s) 99
BstAPI GCANNNNNTGC 1 cut(s) 528
BstAUI TGTACA 1 cut(s) 482
BstC8I GCNNGC 3 cut(s) 9, 19, 714
BstDEI CTNAG 3 cut(s) 12, 323, 668
BstDSI CCRYGG 1 cut(s) 574
BstENI CCTNNNNNAGG 1 cut(s) 656
BstF5I GGATG 3 cut(s) 186, 253, 414
BstFNI CGCG 1 cut(s) 576
BstKTI GATC 2 cut(s) 442, 554
BstMBI GATC 2 cut(s) 439, 551
BstMWI GCNNNNNNNGC 4 cut(s) 56, 528, 570, 579
BstNI CCWGG 1 cut(s) 99
BstNSI RCATGY 2 cut(s) 74, 745
BstSCI CCNGG 1 cut(s) 97
BstUI CGCG 1 cut(s) 576
BstV1I GCAGC 3 cut(s) 76, 98, 591
BstV2I GAAGAC 2 cut(s) 18, 420
BstX2I RGATCY 1 cut(s) 439
BstXI CCANNNNNNTGG 1 cut(s) 82
BstYI RGATCY 1 cut(s) 439
BstZ17I GTATAC 1 cut(s) 241
BsuI GTATCC 1 cut(s) 131
BsuRI GGCC 2 cut(s) 17, 222
BtgI CCRYGG 1 cut(s) 574
BtgZI GCGATG 1 cut(s) 645
BtrI CACGTC 1 cut(s) 232
BtsCI GGATG 3 cut(s) 186, 253, 414
BtsIMutI CAGTG 1 cut(s) 195
BveI ACCTGC 1 cut(s) 536
Cac8I GCNNGC 3 cut(s) 9, 19, 714
Cfr10I RCCGGY 1 cut(s) 712
Cfr42I CCGCGG 1 cut(s) 577
Csp6I GTAC 1 cut(s) 483
CviAII CATG 4 cut(s) 71, 472, 517, 742
CviQI GTAC 1 cut(s) 483
DdeI CTNAG 3 cut(s) 12, 323, 668
DpnI GATC 2 cut(s) 441, 553
DpnII GATC 2 cut(s) 439, 551
Eam1104I CTCTTC 1 cut(s) 99
EarI CTCTTC 1 cut(s) 99
Eco130I CCWWGG 1 cut(s) 81
Eco147I AGGCCT 2 cut(s) 17, 222
Eco32I GATATC 1 cut(s) 64
EcoNI CCTNNNNNAGG 1 cut(s) 656
EcoRII CCWGG 1 cut(s) 97
EcoRV GATATC 1 cut(s) 64
EcoT14I CCWWGG 1 cut(s) 81
EcoT22I ATGCAT 1 cut(s) 473
ErhI CCWWGG 1 cut(s) 81
FaeI CATG 4 cut(s) 74, 475, 520, 745
FatI CATG 4 cut(s) 70, 471, 516, 741
FblI GTMKAC 1 cut(s) 240
Fnu4HI GCNGC 5 cut(s) 87, 90, 574, 577, 580
FokI GGATG 3 cut(s) 193, 260, 421
Fsp4HI GCNGC 5 cut(s) 87, 90, 574, 577, 580
FspBI CTAG 2 cut(s) 443, 621
GluI GCNGC 5 cut(s) 87, 90, 574, 577, 580
GsuI CTGGAG 2 cut(s) 120, 583
HaeIII GGCC 2 cut(s) 17, 222
HapII CCGG 2 cut(s) 418, 713
Hin1II CATG 4 cut(s) 74, 475, 520, 745
HinfI GANTC 1 cut(s) 38
HpaII CCGG 2 cut(s) 418, 713
HphI GGTGA 3 cut(s) 218, 350, 517
Hpy166II GTNNAC 2 cut(s) 241, 509
Hpy188I TCNGA 2 cut(s) 433, 749
Hpy188III TCNNGA 3 cut(s) 53, 443, 600
Hpy8I GTNNAC 2 cut(s) 241, 509
HpyAV CCTTC 4 cut(s) 233, 267, 329, 652
HpyCH4III ACNGT 3 cut(s) 119, 482, 506
HpyCH4IV ACGT 2 cut(s) 231, 673
HpyCH4V TGCA 8 cut(s) 21, 152, 195, 384, 471, 522, 548, 644
HpyF10VI GCNNNNNNNGC 4 cut(s) 56, 528, 570, 579
HpyF3I CTNAG 3 cut(s) 12, 323, 668
HpySE526I ACGT 2 cut(s) 231, 673
Hsp92II CATG 4 cut(s) 74, 475, 520, 745
KroI GCCGGC 1 cut(s) 712
KroNI GCCGGC 1 cut(s) 714
KspI CCGCGG 1 cut(s) 577
Kzo9I GATC 2 cut(s) 439, 551
LguI GCTCTTC 1 cut(s) 99
LmnI GCTCC 1 cut(s) 602
Lsp1109I GCAGC 3 cut(s) 76, 98, 591
LweI GCATC 4 cut(s) 371, 531, 631, 633
MaeI CTAG 2 cut(s) 443, 621
MaeII ACGT 2 cut(s) 231, 673
MaeIII GTNAC 1 cut(s) 646
MalI GATC 2 cut(s) 441, 553
MboI GATC 2 cut(s) 439, 551
MboII GAAGA 5 cut(s) 18, 86, 151, 368, 425
MflI RGATCY 1 cut(s) 439
MluCI AATT 3 cut(s) 211, 296, 341
MmeI TCCRAC 1 cut(s) 91
MnlI CCTC 3 cut(s) 277, 399, 414
Mph1103I ATGCAT 1 cut(s) 473
MroNI GCCGGC 1 cut(s) 712
MroXI GAANNNNTTC 1 cut(s) 256
MseI TTAA 2 cut(s) 290, 345
MspA1I CMGCKG 2 cut(s) 89, 576
MspI CCGG 2 cut(s) 418, 713
MspR9I CCNGG 1 cut(s) 99
Mva1269I GAATGC 3 cut(s) 258, 427, 550
MvaI CCWGG 1 cut(s) 99
MvnI CGCG 1 cut(s) 576
MwoI GCNNNNNNNGC 4 cut(s) 56, 528, 570, 579
NaeI GCCGGC 1 cut(s) 714
NdeII GATC 2 cut(s) 439, 551
NgoMIV GCCGGC 1 cut(s) 712
NlaIII CATG 4 cut(s) 74, 475, 520, 745
NsiI ATGCAT 1 cut(s) 473
NspI RCATGY 2 cut(s) 74, 745
PaqCI CACCTGC 1 cut(s) 536
PceI AGGCCT 2 cut(s) 17, 222
PciSI GCTCTTC 1 cut(s) 99
PctI GAATGC 3 cut(s) 258, 427, 550
PdiI GCCGGC 1 cut(s) 714
PdmI GAANNNNTTC 1 cut(s) 256
PfeI GAWTC 1 cut(s) 38
PfoI TCCNGGA 1 cut(s) 97
PkrI GCNGC 5 cut(s) 88, 91, 575, 578, 581
Psp6I CCWGG 1 cut(s) 97
PspGI CCWGG 1 cut(s) 97
PsuI RGATCY 1 cut(s) 439
PvuII CAGCTG 1 cut(s) 89
RsaI GTAC 1 cut(s) 484
RsaNI GTAC 1 cut(s) 483
SacII CCGCGG 1 cut(s) 577
SapI GCTCTTC 1 cut(s) 99
SaqAI TTAA 2 cut(s) 290, 345
SatI GCNGC 5 cut(s) 87, 90, 574, 577, 580
Sau3AI GATC 2 cut(s) 439, 551
ScrFI CCNGG 1 cut(s) 99
SfaNI GCATC 4 cut(s) 371, 531, 631, 633
Sfr303I CCGCGG 1 cut(s) 577
SgrBI CCGCGG 1 cut(s) 577
Sse9I AATT 3 cut(s) 211, 296, 341
SseBI AGGCCT 2 cut(s) 17, 222
SsiI CCGC 2 cut(s) 574, 576
SspMI CTAG 2 cut(s) 443, 621
StuI AGGCCT 2 cut(s) 17, 222
StyD4I CCNGG 1 cut(s) 97
StyI CCWWGG 1 cut(s) 81
TaaI ACNGT 3 cut(s) 119, 482, 506
TaiI ACGT 2 cut(s) 234, 676
TaqI TCGA 2 cut(s) 333, 554
TasI AATT 3 cut(s) 211, 296, 341
TatI WGTACW 1 cut(s) 482
TauI GCSGC 2 cut(s) 576, 579
TfiI GAWTC 1 cut(s) 38
Tru1I TTAA 2 cut(s) 290, 345
Tru9I TTAA 2 cut(s) 290, 345
TscAI CASTG 1 cut(s) 202
TseI GCWGC 3 cut(s) 86, 89, 579
TspDTI ATGAA 1 cut(s) 471
TspRI CASTG 1 cut(s) 202
XagI CCTNNNNNAGG 1 cut(s) 656
XapI RAATTY 2 cut(s) 296, 341
XbaI TCTAGA 1 cut(s) 442
XceI RCATGY 2 cut(s) 74, 745
XmiI GTMKAC 1 cut(s) 240
XmnI GAANNNNTTC 1 cut(s) 256
XspI CTAG 2 cut(s) 443, 621
Zsp2I ATGCAT 1 cut(s) 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.