MD00G1171600.v1.1

finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0015622
Physical Location & Seq
Forward (+)
39471444 .. 39472511
1068 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1171600.v1.1.491

Sequence Viewer

Length: 1068 bp
ATGTCTAGGAAACTCCATGGATTGGAGCAAAAAGATGGTAATTTGATATATCCACCCCCCTCATTGATATCCCCACCAATACCATCTTCTTCTTCTTCTTCATCTATTGGCAGTATCAGTCCGGTGCTTCTTCTAGTTATAGTAATTGTAGCTGTTATGTTTTTCATCTATGGGTTTGCTCATTTGCTTTGGAGATGTCTCACAAAAGGGTCATCTTCCACCTCAATCTTCCATTCCAATAGATACCCAGAAACCTCTGGCTCTCGAGCTATCCAAAGGCAGCTTCAGCAGCTCTTTCGGCTTCACGATTCGGGCCTCGATCAAGCCGCCATTGATTCTCTCCCTGTGTTCTTCTGCAGAGACATTGTTGGTTCAAAGGAGGAGCCATTTGATTGTGCAGTTTGTCTGTGTGAGTTTTCTGACGAGGACAAGCTGAGGTTGCTTCCTGTATGTGGCCATGCTTTCCACATTGATTGCATTGACACTTGGCTTCTTTCGAATTCGACGTGCCCCCTTTGTAGAGGAACCCTTTTGGGGTCTGGGATTTTGTTGGAGAATCCAGTGTTCGATTCCGGCGGTTCTAGGGAGATGTCAAATGCGGTTGTGAGTGATGGGGAGAACATGTGTTCGAGCGGTCGGAAAGGTTTGGTTATGGAGGAAAGTGTTGGTGAAAAGAGGGTTTTTTCTGTGAGGCTTGGGAAGTTCAAAAGCTTGAATGAAGGTGGAGAAAGTGGGGGGAAAGTGGGGGAGAGCAGTAGGTGTAATTTGGATGCTAGGAGATGTTACTCAATGGGCGCATATCAGTATGTGGTTGGCGATTCAAATCTGCAAATCGCATTGTCCCATGATTGTGATGTCAAGCTTGCCAAAGAGGGAGTTAGTAATGGGAGCTTCTCAGTTAATGGGAATTTGGAGGGGAAGAAAATCAGTGGCAGGCATAAAGGTGAGAGCTTCTCGGTTTCGAAGATTTGGCTTTGGTCTGCAAAGAACAGAGTCCCGGGTTCTTCGAATGCTCAAACCGGTTTGCCTCCTTTTCGTGTCATTTCTCCCATCAATGCTGAAACATGA

Protein Analysis

356

Amino Acids

38.44

Weight (kDa)

7.51

Isoelectric Point (pI)

45.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 131 - 174 8.5e-15 Ring finger domain
zf-RING_11 PF17123 131 - 160 2.4e-09 RING-like zinc finger
zf-rbx1 PF12678 132 - 174 8.2e-10 RING-H2 zinc finger domain
zf-C3HC4 PF00097 132 - 173 1.6e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 132 - 173 4.6e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015622)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g05060
malus_domestica MD00G1171600.v1.1 MD14G1184200.v1.1
prunus_persica Prupe.5G176800_v2.0.a1
pyrus_communis pycom06g15800 pycom14g15320
rosa_chinensis RchiOBHm_Chr7g0193981
rosa_laevigata RLG00000004226
rosa_multiflora Rmu_sc0002389.1_g000005
rosa_roxburghii Rroxscaffold_3G00261650
rosa_rugosa Rorug07G0013000
rosa_samantha Rh7AG140100 Rh7BG140500 Rh7CG144100 Rh7DG141400
rosa_wichuraiana Rw7G011950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 22
AccBSI CCGCTC 1 cut(s) 633
AciI CCGC 4 cut(s) 327, 576, 599, 633
AcoI YGGCCR 1 cut(s) 454
AcsI RAATTY 2 cut(s) 499, 907
AcuI CTGAAG 1 cut(s) 269
AfiI CCNNNNNNNGG 3 cut(s) 22, 452, 534
AflIII ACRYGT 1 cut(s) 621
AgeI ACCGGT 1 cut(s) 1019
AgsI TTSAA 4 cut(s) 375, 706, 715, 822
AjiI CACGTC 1 cut(s) 507
AjuI GAANNNNNNNTTGG 4 cut(s) 70, 102, 267, 299
AluBI AGCT 9 cut(s) 152, 269, 283, 292, 433, 711, 862, 891, 951
AluI AGCT 9 cut(s) 152, 269, 283, 292, 433, 711, 862, 891, 951
Alw26I GTCTC 2 cut(s) 203, 354
Ama87I CYCGRG 2 cut(s) 264, 997
AoxI GGCC 2 cut(s) 313, 454
ApeKI GCWGC 2 cut(s) 280, 289
ApoI RAATTY 2 cut(s) 499, 907
AsiGI ACCGGT 1 cut(s) 1019
AspLEI GCGC 1 cut(s) 797
AspS9I GGNCC 1 cut(s) 313
AsuC2I CCSGG 2 cut(s) 998, 999
AsuHPI GGTGA 2 cut(s) 680, 956
AsuII TTCGAA 3 cut(s) 497, 962, 1007
AvaI CYCGRG 2 cut(s) 264, 997
BaeGI GKGCMC 1 cut(s) 512
BalI TGGCCA 1 cut(s) 456
BbvCI CCTCAGC 1 cut(s) 434
BbvI GCAGC 2 cut(s) 292, 301
BccI CCATC 4 cut(s) 29, 91, 605, 1058
BcgI CGANNNNNNTGC 2 cut(s) 278, 312
BcnI CCSGG 2 cut(s) 998, 999
BcoDI GTCTC 2 cut(s) 203, 354
BfaI CTAG 4 cut(s) 6, 134, 582, 774
BfmI CTRYAG 1 cut(s) 355
BisI GCNGC 3 cut(s) 281, 290, 327
BlsI GCNGC 3 cut(s) 282, 291, 328
Bme1390I CCNGG 2 cut(s) 998, 999
BmeT110I CYCGRG 2 cut(s) 264, 997
BmgBI CACGTC 1 cut(s) 507
BmgT120I GGNCC 1 cut(s) 313
BmiI GGNNCC 2 cut(s) 384, 526
BmrFI CCNGG 2 cut(s) 998, 999
BmsI GCATC 1 cut(s) 760
BplI GAGNNNNNCTC 2 cut(s) 938, 970
Bpu10I CCTNAGC 1 cut(s) 434
Bpu14I TTCGAA 3 cut(s) 497, 962, 1007
BpuMI CCSGG 2 cut(s) 998, 999
BsaBI GATNNNNATC 1 cut(s) 822
BsaJI CCNNGG 2 cut(s) 16, 997
BsaWI WCCGGW 2 cut(s) 121, 1019
BsaXI ACNNNNNCTCC 2 cut(s) 608, 638
Bsc4I CCNNNNNNNGG 3 cut(s) 22, 452, 534
Bse118I RCCGGY 1 cut(s) 1019
Bse1I ACTGG 1 cut(s) 560
Bse8I GATNNNNATC 1 cut(s) 822
BseDI CCNNGG 2 cut(s) 16, 997
BseGI GGATG 1 cut(s) 775
BseJI GATNNNNATC 1 cut(s) 822
BseLI CCNNNNNNNGG 3 cut(s) 22, 452, 534
BseMII CTCAG 2 cut(s) 425, 909
BseNI ACTGG 1 cut(s) 560
BseRI GAGGAG 1 cut(s) 395
BseSI GKGCMC 1 cut(s) 512
BseXI GCAGC 2 cut(s) 292, 301
BsgI GTGCAG 1 cut(s) 417
Bsh1285I CGRYCG 1 cut(s) 637
BshFI GGCC 2 cut(s) 315, 456
BshTI ACCGGT 1 cut(s) 1019
BsiEI CGRYCG 1 cut(s) 637
BsiHKCI CYCGRG 2 cut(s) 264, 997
BsiSI CCGG 4 cut(s) 122, 573, 998, 1020
BslFI GGGAC 2 cut(s) 826, 980
BslI CCNNNNNNNGG 3 cut(s) 22, 452, 534
BsmAI GTCTC 2 cut(s) 203, 354
BsmFI GGGAC 2 cut(s) 826, 980
BsmI GAATGC 1 cut(s) 1015
BsnI GGCC 2 cut(s) 315, 456
BsoBI CYCGRG 2 cut(s) 264, 997
Bsp119I TTCGAA 3 cut(s) 497, 962, 1007
Bsp1286I GDGCHC 1 cut(s) 512
Bsp143I GATC 1 cut(s) 319
Bsp19I CCATGG 1 cut(s) 16
BspACI CCGC 4 cut(s) 327, 576, 599, 633
BspANI GGCC 2 cut(s) 315, 456
BspCNI CTCAG 2 cut(s) 426, 908
BspLI GGNNCC 2 cut(s) 384, 526
BspMAI CTGCAG 1 cut(s) 359
BspT104I TTCGAA 3 cut(s) 497, 962, 1007
BsrBI CCGCTC 1 cut(s) 633
BsrFI RCCGGY 1 cut(s) 1019
BsrI ACTGG 1 cut(s) 560
BssAI RCCGGY 1 cut(s) 1019
BssECI CCNNGG 2 cut(s) 16, 997
BssMI GATC 1 cut(s) 319
BssT1I CCWWGG 1 cut(s) 16
BstBI TTCGAA 3 cut(s) 497, 962, 1007
BstC8I GCNNGC 2 cut(s) 864, 935
BstDEI CTNAG 2 cut(s) 434, 895
BstDSI CCRYGG 1 cut(s) 16
BstF5I GGATG 1 cut(s) 775
BstHHI GCGC 1 cut(s) 797
BstKTI GATC 1 cut(s) 322
BstMAI GTCTC 2 cut(s) 203, 354
BstMBI GATC 1 cut(s) 319
BstMCI CGRYCG 1 cut(s) 637
BstMWI GCNNNNNNNGC 4 cut(s) 286, 289, 298, 439
BstNSI RCATGY 1 cut(s) 625
BstSCI CCNGG 2 cut(s) 996, 997
BstSFI CTRYAG 1 cut(s) 355
BstSLI GKGCMC 1 cut(s) 512
BstV1I GCAGC 2 cut(s) 292, 301
BsuRI GGCC 2 cut(s) 315, 456
BtgI CCRYGG 1 cut(s) 16
BtrI CACGTC 1 cut(s) 507
BtsCI GGATG 1 cut(s) 775
BtsIMutI CAGTG 2 cut(s) 567, 934
Cac8I GCNNGC 2 cut(s) 864, 935
CfoI GCGC 1 cut(s) 797
Cfr10I RCCGGY 1 cut(s) 1019
Cfr13I GGNCC 1 cut(s) 313
Cfr9I CCCGGG 1 cut(s) 997
CspAI ACCGGT 1 cut(s) 1019
CspCI CAANNNNNGTGG 2 cut(s) 455, 490
CviAII CATG 5 cut(s) 17, 458, 622, 845, 1065
DdeI CTNAG 2 cut(s) 434, 895
DpnI GATC 1 cut(s) 321
DpnII GATC 1 cut(s) 319
EaeI YGGCCR 1 cut(s) 454
Eco130I CCWWGG 1 cut(s) 16
Eco32I GATATC 1 cut(s) 69
Eco57I CTGAAG 1 cut(s) 269
Eco88I CYCGRG 2 cut(s) 264, 997
EcoRI GAATTC 1 cut(s) 499
EcoRV GATATC 1 cut(s) 69
EcoT14I CCWWGG 1 cut(s) 16
ErhI CCWWGG 1 cut(s) 16
FaeI CATG 5 cut(s) 20, 461, 625, 848, 1068
FaqI GGGAC 2 cut(s) 826, 980
FatI CATG 5 cut(s) 16, 457, 621, 844, 1064
Fnu4HI GCNGC 3 cut(s) 281, 290, 327
FokI GGATG 1 cut(s) 782
Fsp4HI GCNGC 3 cut(s) 281, 290, 327
FspBI CTAG 4 cut(s) 6, 134, 582, 774
GlaI GCGC 1 cut(s) 796
GluI GCNGC 3 cut(s) 281, 290, 327
HaeIII GGCC 2 cut(s) 315, 456
HapII CCGG 4 cut(s) 122, 573, 998, 1020
HhaI GCGC 1 cut(s) 797
Hin1II CATG 5 cut(s) 20, 461, 625, 848, 1068
Hin6I GCGC 1 cut(s) 795
HinP1I GCGC 1 cut(s) 795
HindIII AAGCTT 2 cut(s) 709, 860
HinfI GANTC 6 cut(s) 308, 335, 556, 569, 818, 993
HpaII CCGG 4 cut(s) 122, 573, 998, 1020
HphI GGTGA 2 cut(s) 680, 956
Hpy188I TCNGA 2 cut(s) 421, 639
Hpy188III TCNNGA 2 cut(s) 264, 305
Hpy99I CGWCG 1 cut(s) 508
HpyAV CCTTC 1 cut(s) 713
HpyCH4IV ACGT 1 cut(s) 506
HpyCH4V TGCA 5 cut(s) 357, 398, 477, 829, 983
HpyF10VI GCNNNNNNNGC 4 cut(s) 286, 289, 298, 439
HpyF3I CTNAG 2 cut(s) 434, 895
HpySE526I ACGT 1 cut(s) 506
Hsp92II CATG 5 cut(s) 20, 461, 625, 848, 1068
HspAI GCGC 1 cut(s) 795
Kzo9I GATC 1 cut(s) 319
LmnI GCTCC 3 cut(s) 25, 382, 888
Lsp1109I GCAGC 2 cut(s) 292, 301
LweI GCATC 1 cut(s) 760
MaeI CTAG 4 cut(s) 6, 134, 582, 774
MaeII ACGT 1 cut(s) 506
MaeIII GTNAC 1 cut(s) 782
MalI GATC 1 cut(s) 321
MbiI CCGCTC 1 cut(s) 633
MboI GATC 1 cut(s) 319
MhlI GDGCHC 1 cut(s) 512
MlsI TGGCCA 1 cut(s) 456
MluCI AATT 5 cut(s) 40, 144, 499, 763, 907
MluNI TGGCCA 1 cut(s) 456
MlyI GAGTC 1 cut(s) 1002
MmeI TCCRAC 2 cut(s) 531, 617
Mox20I TGGCCA 1 cut(s) 456
MscI TGGCCA 1 cut(s) 456
MseI TTAA 1 cut(s) 900
MslI CAYNNNNRTG 2 cut(s) 849, 942
Msp20I TGGCCA 1 cut(s) 456
MspI CCGG 4 cut(s) 122, 573, 998, 1020
MspR9I CCNGG 2 cut(s) 998, 999
Mva1269I GAATGC 1 cut(s) 1015
MwoI GCNNNNNNNGC 4 cut(s) 286, 289, 298, 439
NciI CCSGG 2 cut(s) 998, 999
NcoI CCATGG 1 cut(s) 16
NdeII GATC 1 cut(s) 319
NlaIII CATG 5 cut(s) 20, 461, 625, 848, 1068
NlaIV GGNNCC 2 cut(s) 384, 526
NspI RCATGY 1 cut(s) 625
NspV TTCGAA 3 cut(s) 497, 962, 1007
PaeR7I CTCGAG 1 cut(s) 264
PciI ACATGT 1 cut(s) 621
PcsI WCGNNNNNNNCGW 1 cut(s) 503
PctI GAATGC 1 cut(s) 1015
PfeI GAWTC 5 cut(s) 308, 335, 556, 569, 818
PflMI CCANNNNNTGG 1 cut(s) 22
PinAI ACCGGT 1 cut(s) 1019
PkrI GCNGC 3 cut(s) 282, 291, 328
PleI GAGTC 1 cut(s) 1001
PpsI GAGTC 1 cut(s) 1001
PscI ACATGT 1 cut(s) 621
PspN4I GGNNCC 2 cut(s) 384, 526
PspPI GGNCC 1 cut(s) 313
PstI CTGCAG 1 cut(s) 359
RseI CAYNNNNRTG 2 cut(s) 849, 942
SaqAI TTAA 1 cut(s) 900
SatI GCNGC 3 cut(s) 281, 290, 327
Sau3AI GATC 1 cut(s) 319
Sau96I GGNCC 1 cut(s) 313
SchI GAGTC 1 cut(s) 1002
ScrFI CCNGG 2 cut(s) 998, 999
SduI GDGCHC 1 cut(s) 512
SfaNI GCATC 1 cut(s) 760
SfcI CTRYAG 1 cut(s) 355
Sfr274I CTCGAG 1 cut(s) 264
SfuI TTCGAA 3 cut(s) 497, 962, 1007
SlaI CTCGAG 1 cut(s) 264
SmaI CCCGGG 1 cut(s) 999
SmiMI CAYNNNNRTG 2 cut(s) 849, 942
SmlI CTYRAG 1 cut(s) 264
SmoI CTYRAG 1 cut(s) 264
Sse9I AATT 5 cut(s) 40, 144, 499, 763, 907
SsiI CCGC 4 cut(s) 327, 576, 599, 633
SspMI CTAG 4 cut(s) 6, 134, 582, 774
StyD4I CCNGG 2 cut(s) 996, 997
StyI CCWWGG 1 cut(s) 16
TaiI ACGT 1 cut(s) 509
TaqI TCGA 8 cut(s) 265, 318, 497, 503, 567, 629, 962, 1007
TasI AATT 5 cut(s) 40, 144, 499, 763, 907
TauI GCSGC 1 cut(s) 329
TfiI GAWTC 5 cut(s) 308, 335, 556, 569, 818
Tru1I TTAA 1 cut(s) 900
Tru9I TTAA 1 cut(s) 900
TscAI CASTG 2 cut(s) 567, 934
TseI GCWGC 2 cut(s) 280, 289
TspDTI ATGAA 3 cut(s) 90, 154, 732
TspMI CCCGGG 1 cut(s) 997
TspRI CASTG 2 cut(s) 567, 934
Van91I CCANNNNNTGG 1 cut(s) 22
XapI RAATTY 2 cut(s) 499, 907
XceI RCATGY 1 cut(s) 625
XhoI CTCGAG 1 cut(s) 264
XmaI CCCGGG 1 cut(s) 997
XspI CTAG 4 cut(s) 6, 134, 582, 774
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.