Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0014359

Physical Location & Seq

Genomic Coordinates Forward (+)

39574511 .. 39576858

Gene Structure 2348 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1172500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1296 bp

ATGAGCTTCCCTCCTTCTGCATCAGGCGCGATGAAAGCAGTTCGAGGAAAGCTACATTTTCCTGTTATCATGGTTTGCGCTATCGCCTTTATCAGTCTCCTCTACGCCGAACGAACTAGCATCATTCTTTCCTCCAACCCTTTTTTCAAAATTAAATCATGTTCCAAACCCGAAGCCATTACGAAAAATAAAAACAGAAATGCTACCGAGGATAAGTTAAGGGATTCCATATTAGATGATAGGTTTGCTTTCAACCCCGAGGAGTGCAGTGTTGAGAATGGAGAATGGGTGTTCAACCGTTCGATCAAGCCTCTTTACTCAGACAGAAGTTGCCCGTATCTTGATAGACAAGTTTCGTGTGTCAAGAATGGACGTCCGGATTCTGGCTATCGCTACTGGCAATGGCAGATTGAAGATTGTACATTGCCAAGATTTGATCCAGAAGTTACACTTAAGAAACTCAGAGGAAAGAGACTACTTTTTGTTGGGGATTCTTTGCAAAGAGGTCAATGGCAATCTTTAGTCTGTATGGTCGAATCCATCATACCCGAGAACCAAAAATCCATTCAACGAGGGCGGTCTCATACAGTCTTCAGAGCCAAGGAGTATAATGCTACTATTGAATTCTACTGGGCGCCATTTCTGGTTGAGTCCAATTCTGATGAACATATAATAGGAAATCCAAGGGAAAGAATACTAAAAGTGGATTCAGTTGCCAAGCATGCGAAATACTGGACAGGAGTTGATATCCTTGCGTTCAACACTTACGTTTGGTGGATGAGCGGCTACACGATCAAATCATACTGGGGATCGTTTCCAAATGGTGAAGAAGGGTATGAAGAATTGGATGCACCGGTTGCCTACAGAATGGCATTGAAGACATGGGCAAACTGGGTCGACTTAAATATCAATCCGAACAAGACTCGTGTTTTTTTCACTACTATGTCCCCTACACACATGAGAAGTGCAGACTGGGAAAATCCAGATGGGATGAAATGCTTCAACGAAACAAAGCCGTACATGAAAAAGGGATTCTGGGGAACAGGTTCGGACAAGAGGGTCATGCGCGTGGTGTCCGAAGTAATCCGCAAAATGAAAGTTCCGGTTTCAGTACTTAATGTAACGCAGATGTCAAATCACAGAGTTGATGCTCACACAAAAGTTTACACCGAAACCGGAGGCAACCTACTAACTGATGAACAAAAGGCTGATGTGCTGCACAATTCAGATTGTATACATTGGTGCCTTCCTGGAGTTCCAGACGCATGGAATCAAATTTTTATGGCACATTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005886 GO:0005975 GO:0005976 GO:0006073 GO:0008150 GO:0008152 GO:0008374 GO:0009058 GO:0009059 GO:0009250 GO:0009664 GO:0009827 GO:0009987 GO:0010383 GO:0010393 GO:0010410 GO:0012505 GO:0016020 GO:0016043 GO:0016051 GO:0016407 GO:0016413 GO:0016740 GO:0016746 GO:0016747 GO:0017144 GO:0030243 GO:0030244 GO:0033692 GO:0034637 GO:0034645 GO:0042545 GO:0042546 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044036 GO:0044038 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0045229 GO:0045488 GO:0045489 GO:0045491 GO:0045492 GO:0051273 GO:0051274 GO:0070589 GO:0070592 GO:0071554 GO:0071555 GO:0071669 GO:0071704 GO:0071840 GO:0071944 GO:1901576 GO:1990538

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PC-Esterase PMR5N

Protein Analysis

432

Amino Acids

49.69

Weight (kDa)

8.84

Isoelectric Point (pI)

38.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMR5N	PF14416	87 - 140	1.8e-18	PMR5 N terminal Domain
PC-Esterase	PF13839	142 - 429	7.8e-95	GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014359)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G01360 AT5G01360
fragaria_vesca	FvH4_6g11230 FvH4_6g11230
malus_domestica	MD00G1172500.v1.1 MD12G1171500.v1.1
prunus_persica	Prupe.6G279700_v2.0.a1
pyrus_communis	pycom04g14210 pycom12g16280
rosa_chinensis	RchiOBHm_Chr3g0462741
rosa_laevigata	RLG00000024836
rosa_multiflora	Rmu_sc0000766.1_g000003 Rmu_sc0011057.1_g000007
rosa_roxburghii	Rroxscaffold_6G00417240
rosa_rugosa	Rorug03G0058900
rosa_samantha	Rh3DG122300
rosa_wichuraiana	Rw3G009900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1058
AatII	GACGTC	1 cut(s)	376
AccB1I	GGYRCC	2 cut(s)	634, 1242
AccBSI	CCGCTC	1 cut(s)	783
AccI	GTMKAC	2 cut(s)	897, 1234
AccII	CGCG	2 cut(s)	29, 1068
AccIII	TCCGGA	1 cut(s)	376
AciI	CCGC	3 cut(s)	577, 783, 1087
AclWI	GGATC	2 cut(s)	431, 817
AcsI	RAATTY	2 cut(s)	623, 1275
AcuI	CTGAAG	1 cut(s)	577
AcyI	GRCGYC	2 cut(s)	373, 635
AfaI	GTAC	3 cut(s)	421, 1019, 1113
AfiI	CCNNNNNNNGG	1 cut(s)	383
AflII	CTTAAG	1 cut(s)	452
AgeI	ACCGGT	1 cut(s)	853
AgsI	TTSAA	9 cut(s)	148, 253, 295, 413, 569, 623, 760, 877, 1003
AjnI	CCWGG	1 cut(s)	1249
AjuI	GAANNNNNNNTTGG	4 cut(s)	128, 160, 549, 581
AloI	GAACNNNNNNTCC	2 cut(s)	545, 577
AluBI	AGCT	2 cut(s)	6, 52
AluI	AGCT	2 cut(s)	6, 52
Alw26I	GTCTC	3 cut(s)	101, 466, 585
AlwI	GGATC	2 cut(s)	431, 817
Ama87I	CYCGRG	2 cut(s)	257, 548
Aor13HI	TCCGGA	1 cut(s)	376
ApeKI	GCWGC	1 cut(s)	1216
ApoI	RAATTY	2 cut(s)	623, 1275
AsiGI	ACCGGT	1 cut(s)	853
Asp700I	GAANNNNTTC	2 cut(s)	998, 1045
AspLEI	GCGC	4 cut(s)	29, 80, 637, 1068
AsuHPI	GGTGA	1 cut(s)	836
AvaI	CYCGRG	2 cut(s)	257, 548
BanI	GGYRCC	2 cut(s)	634, 1242
BauI	CACGAG	1 cut(s)	924
BbsI	GAAGAC	2 cut(s)	583, 884
BbvI	GCAGC	1 cut(s)	1203
BccI	CCATC	2 cut(s)	548, 980
BceAI	ACGGC	1 cut(s)	1000
BciT130I	CCWGG	1 cut(s)	1251
BcoDI	GTCTC	3 cut(s)	101, 466, 585
BfaI	CTAG	1 cut(s)	117
BfmI	CTRYAG	1 cut(s)	862
BfoI	RGCGCY	1 cut(s)	638
BfrI	CTTAAG	1 cut(s)	452
BisI	GCNGC	2 cut(s)	784, 1217
BlsI	GCNGC	2 cut(s)	785, 1218
BmcAI	AGTACT	1 cut(s)	1113
Bme1390I	CCNGG	1 cut(s)	1251
BmeT110I	CYCGRG	2 cut(s)	257, 548
BmiI	GGNNCC	2 cut(s)	636, 1244
BmrFI	CCNGG	1 cut(s)	1251
BmrI	ACTGGG	4 cut(s)	640, 814, 901, 982
BmsI	GCATC	4 cut(s)	29, 129, 838, 1138
BmuI	ACTGGG	4 cut(s)	640, 814, 901, 982
BpiI	GAAGAC	2 cut(s)	583, 884
BplI	GAGNNNNNCTC	1 cut(s)	27
BpmI	CTGGAG	1 cut(s)	1272
BsaHI	GRCGYC	2 cut(s)	373, 635
BsaI	GGTCTC	1 cut(s)	585
BsaJI	CCNNGG	4 cut(s)	207, 258, 600, 683
BsaWI	WCCGGW	4 cut(s)	376, 853, 1102, 1175
BsaXI	ACNNNNNCTCC	2 cut(s)	273, 303
Bsc4I	CCNNNNNNNGG	1 cut(s)	383
Bse118I	RCCGGY	1 cut(s)	853
Bse1I	ACTGG	6 cut(s)	401, 635, 737, 809, 896, 977
Bse3DI	GCAATG	2 cut(s)	407, 422
BseAI	TCCGGA	1 cut(s)	376
BseBI	CCWGG	1 cut(s)	1251
BseDI	CCNNGG	4 cut(s)	207, 258, 600, 683
BseGI	GGATG	3 cut(s)	783, 853, 996
BseLI	CCNNNNNNNGG	1 cut(s)	383
BseMI	GCAATG	2 cut(s)	407, 422
BseMII	CTCAG	2 cut(s)	333, 475
BseNI	ACTGG	6 cut(s)	401, 635, 737, 809, 896, 977
BseRI	GAGGAG	2 cut(s)	89, 275
BseXI	GCAGC	1 cut(s)	1203
BsgI	GTGCAG	3 cut(s)	286, 987, 1202
Bsh1236I	CGCG	2 cut(s)	29, 1068
BshNI	GGYRCC	2 cut(s)	634, 1242
BshTI	ACCGGT	1 cut(s)	853
BsiHKCI	CYCGRG	2 cut(s)	257, 548
BsiSI	CCGG	4 cut(s)	377, 854, 1103, 1176
BslFI	GGGAC	1 cut(s)	931
BslI	CCNNNNNNNGG	1 cut(s)	383
BsmAI	GTCTC	3 cut(s)	101, 466, 585
BsmFI	GGGAC	1 cut(s)	931
Bso31I	GGTCTC	1 cut(s)	585
BsoBI	CYCGRG	2 cut(s)	257, 548
Bsp13I	TCCGGA	1 cut(s)	376
Bsp1407I	TGTACA	1 cut(s)	419
Bsp143I	GATC	4 cut(s)	303, 436, 792, 809
BspACI	CCGC	3 cut(s)	577, 783, 1087
BspCNI	CTCAG	2 cut(s)	332, 474
BspEI	TCCGGA	1 cut(s)	376
BspFNI	CGCG	2 cut(s)	29, 1068
BspLI	GGNNCC	2 cut(s)	636, 1244
BspPI	GGATC	2 cut(s)	431, 817
BspT107I	GGYRCC	2 cut(s)	634, 1242
BspTI	CTTAAG	1 cut(s)	452
BspTNI	GGTCTC	1 cut(s)	585
BsrBI	CCGCTC	1 cut(s)	783
BsrDI	GCAATG	2 cut(s)	407, 422
BsrFI	RCCGGY	1 cut(s)	853
BsrGI	TGTACA	1 cut(s)	419
BsrI	ACTGG	6 cut(s)	401, 635, 737, 809, 896, 977
BssAI	RCCGGY	1 cut(s)	853
BssECI	CCNNGG	4 cut(s)	207, 258, 600, 683
BssMI	GATC	4 cut(s)	303, 436, 792, 809
BssNAI	GTATAC	1 cut(s)	1235
BssNI	GRCGYC	2 cut(s)	373, 635
BssSI	CACGAG	1 cut(s)	924
BssT1I	CCWWGG	2 cut(s)	600, 683
Bst1107I	GTATAC	1 cut(s)	1235
Bst2BI	CACGAG	1 cut(s)	924
Bst2UI	CCWGG	1 cut(s)	1251
Bst4CI	ACNGT	2 cut(s)	299, 589
BstACI	GRCGYC	2 cut(s)	373, 635
BstAFI	CTTAAG	1 cut(s)	452
BstAPI	GCANNNNNTGC	1 cut(s)	857
BstAUI	TGTACA	1 cut(s)	419
BstC8I	GCNNGC	1 cut(s)	723
BstDEI	CTNAG	2 cut(s)	319, 461
BstF5I	GGATG	3 cut(s)	783, 853, 996
BstFNI	CGCG	2 cut(s)	29, 1068
BstH2I	RGCGCY	1 cut(s)	638
BstHHI	GCGC	4 cut(s)	29, 80, 637, 1068
BstKTI	GATC	4 cut(s)	306, 439, 795, 812
BstMAI	GTCTC	3 cut(s)	101, 466, 585
BstMBI	GATC	4 cut(s)	303, 436, 792, 809
BstMWI	GCNNNNNNNGC	4 cut(s)	26, 35, 722, 857
BstNI	CCWGG	1 cut(s)	1251
BstNSI	RCATGY	1 cut(s)	725
BstSCI	CCNGG	1 cut(s)	1249
BstSFI	CTRYAG	1 cut(s)	862
BstUI	CGCG	2 cut(s)	29, 1068
BstV1I	GCAGC	1 cut(s)	1203
BstV2I	GAAGAC	2 cut(s)	583, 884
BstXI	CCANNNNNNTGG	1 cut(s)	1266
BstZ17I	GTATAC	1 cut(s)	1235
BtgZI	GCGATG	1 cut(s)	44
BtsCI	GGATG	3 cut(s)	783, 853, 996
BtsI	GCAGTG	1 cut(s)	274
BtsIMutI	CAGTG	1 cut(s)	274
Cac8I	GCNNGC	1 cut(s)	723
CfoI	GCGC	4 cut(s)	29, 80, 637, 1068
Cfr10I	RCCGGY	1 cut(s)	853
CseI	GACGC	1 cut(s)	1271
Csp6I	GTAC	3 cut(s)	420, 1018, 1112
CspAI	ACCGGT	1 cut(s)	853
CviAII	CATG	8 cut(s)	70, 159, 722, 882, 958, 1021, 1063, 1266
CviJI	RGCY	9 cut(s)	6, 52, 176, 310, 387, 599, 786, 1015, 1208
CviKI_1	RGCY	9 cut(s)	6, 52, 176, 310, 387, 599, 786, 1015, 1208
CviQI	GTAC	3 cut(s)	420, 1018, 1112
DdeI	CTNAG	2 cut(s)	319, 461
DinI	GGCGCC	1 cut(s)	636
DpnI	GATC	4 cut(s)	305, 438, 794, 811
DpnII	GATC	4 cut(s)	303, 436, 792, 809
DrdI	GACNNNNNNGTC	1 cut(s)	1058
DseDI	GACNNNNNNGTC	1 cut(s)	1058
Eco130I	CCWWGG	2 cut(s)	600, 683
Eco31I	GGTCTC	1 cut(s)	585
Eco32I	GATATC	1 cut(s)	748
Eco57I	CTGAAG	1 cut(s)	577
Eco88I	CYCGRG	2 cut(s)	257, 548
EcoRI	GAATTC	1 cut(s)	623
EcoRII	CCWGG	1 cut(s)	1249
EcoRV	GATATC	1 cut(s)	748
EcoT14I	CCWWGG	2 cut(s)	600, 683
EgeI	GGCGCC	1 cut(s)	636
EheI	GGCGCC	1 cut(s)	636
ErhI	CCWWGG	2 cut(s)	600, 683
FaeI	CATG	8 cut(s)	73, 162, 725, 885, 961, 1024, 1066, 1269
FalI	AAGNNNNNCTT	2 cut(s)	435, 467
FaqI	GGGAC	1 cut(s)	931
FatI	CATG	8 cut(s)	69, 158, 721, 881, 957, 1020, 1062, 1265
FblI	GTMKAC	2 cut(s)	897, 1234
Fnu4HI	GCNGC	2 cut(s)	784, 1217
FokI	GGATG	3 cut(s)	790, 860, 1003
Fsp4HI	GCNGC	2 cut(s)	784, 1217
FspBI	CTAG	1 cut(s)	117
GlaI	GCGC	4 cut(s)	28, 79, 636, 1067
GluI	GCNGC	2 cut(s)	784, 1217
GsuI	CTGGAG	1 cut(s)	1272
HaeII	RGCGCY	1 cut(s)	638
HapII	CCGG	4 cut(s)	377, 854, 1103, 1176
HgaI	GACGC	1 cut(s)	1271
HhaI	GCGC	4 cut(s)	29, 80, 637, 1068
Hin1I	GRCGYC	2 cut(s)	373, 635
Hin1II	CATG	8 cut(s)	73, 162, 725, 885, 961, 1024, 1066, 1269
Hin6I	GCGC	4 cut(s)	27, 78, 635, 1066
HinP1I	GCGC	4 cut(s)	27, 78, 635, 1066
HincII	GTYRAC	1 cut(s)	898
HindII	GTYRAC	1 cut(s)	898
HinfI	GANTC	9 cut(s)	224, 380, 491, 536, 650, 707, 922, 1032, 1270
HpaII	CCGG	4 cut(s)	377, 854, 1103, 1176
HphI	GGTGA	1 cut(s)	836
Hpy166II	GTNNAC	3 cut(s)	898, 1165, 1235
Hpy188I	TCNGA	8 cut(s)	322, 464, 596, 661, 915, 1051, 1078, 1228
Hpy188III	TCNNGA	6 cut(s)	341, 364, 377, 440, 983, 1259
Hpy8I	GTNNAC	3 cut(s)	898, 1165, 1235
HpyAV	CCTTC	3 cut(s)	24, 824, 1256
HpyCH4III	ACNGT	2 cut(s)	299, 589
HpyCH4IV	ACGT	2 cut(s)	373, 768
HpyCH4V	TGCA	6 cut(s)	20, 267, 499, 851, 968, 1219
HpyF10VI	GCNNNNNNNGC	4 cut(s)	26, 35, 722, 857
HpyF3I	CTNAG	2 cut(s)	319, 461
HpySE526I	ACGT	2 cut(s)	373, 768
Hsp92I	GRCGYC	2 cut(s)	373, 635
Hsp92II	CATG	8 cut(s)	73, 162, 725, 885, 961, 1024, 1066, 1269
HspAI	GCGC	4 cut(s)	27, 78, 635, 1066
KasI	GGCGCC	1 cut(s)	634
Kpn2I	TCCGGA	1 cut(s)	376
Kzo9I	GATC	4 cut(s)	303, 436, 792, 809
Lsp1109I	GCAGC	1 cut(s)	1203
LweI	GCATC	4 cut(s)	29, 129, 838, 1138
MaeI	CTAG	1 cut(s)	117
MaeII	ACGT	2 cut(s)	373, 768
MaeIII	GTNAC	2 cut(s)	445, 1120
MalI	GATC	4 cut(s)	305, 438, 794, 811
MbiI	CCGCTC	1 cut(s)	783
MboI	GATC	4 cut(s)	303, 436, 792, 809
MboII	GAAGA	5 cut(s)	425, 583, 839, 851, 889
MluCI	AATT	6 cut(s)	150, 623, 655, 842, 1222, 1275
Mly113I	GGCGCC	1 cut(s)	635
MlyI	GAGTC	2 cut(s)	659, 916
MmeI	TCCRAC	1 cut(s)	159
MroI	TCCGGA	1 cut(s)	376
MroXI	GAANNNNTTC	2 cut(s)	998, 1045
MseI	TTAA	5 cut(s)	153, 218, 453, 902, 1116
MslI	CAYNNNNRTG	2 cut(s)	941, 1067
MspCI	CTTAAG	1 cut(s)	452
MspI	CCGG	4 cut(s)	377, 854, 1103, 1176
MspR9I	CCNGG	1 cut(s)	1251
MvaI	CCWGG	1 cut(s)	1251
MvnI	CGCG	2 cut(s)	29, 1068
MwoI	GCNNNNNNNGC	4 cut(s)	26, 35, 722, 857
NarI	GGCGCC	1 cut(s)	635
NdeII	GATC	4 cut(s)	303, 436, 792, 809
NlaIII	CATG	8 cut(s)	73, 162, 725, 885, 961, 1024, 1066, 1269
NlaIV	GGNNCC	2 cut(s)	636, 1244
NspI	RCATGY	1 cut(s)	725
PaeI	GCATGC	1 cut(s)	725
PdmI	GAANNNNTTC	2 cut(s)	998, 1045
PfeI	GAWTC	7 cut(s)	224, 380, 491, 536, 707, 1032, 1270
PfoI	TCCNGGA	1 cut(s)	1249
PinAI	ACCGGT	1 cut(s)	853
PkrI	GCNGC	2 cut(s)	785, 1218
PleI	GAGTC	2 cut(s)	658, 916
PluTI	GGCGCC	1 cut(s)	638
PpsI	GAGTC	2 cut(s)	658, 916
Psp6I	CCWGG	1 cut(s)	1249
PspGI	CCWGG	1 cut(s)	1249
PspN4I	GGNNCC	2 cut(s)	636, 1244
RsaI	GTAC	3 cut(s)	421, 1019, 1113
RsaNI	GTAC	3 cut(s)	420, 1018, 1112
RseI	CAYNNNNRTG	2 cut(s)	941, 1067
SalI	GTCGAC	1 cut(s)	896
SaqAI	TTAA	5 cut(s)	153, 218, 453, 902, 1116
SatI	GCNGC	2 cut(s)	784, 1217
Sau3AI	GATC	4 cut(s)	303, 436, 792, 809
ScaI	AGTACT	1 cut(s)	1113
SchI	GAGTC	2 cut(s)	659, 916
ScrFI	CCNGG	1 cut(s)	1251
SetI	ASST	8 cut(s)	8, 54, 245, 376, 508, 771, 1048, 1188
SfaNI	GCATC	4 cut(s)	29, 129, 838, 1138
SfcI	CTRYAG	1 cut(s)	862
SfoI	GGCGCC	1 cut(s)	636
SmiMI	CAYNNNNRTG	2 cut(s)	941, 1067
SmlI	CTYRAG	1 cut(s)	452
SmoI	CTYRAG	1 cut(s)	452
SphI	GCATGC	1 cut(s)	725
Sse9I	AATT	6 cut(s)	150, 623, 655, 842, 1222, 1275
SsiI	CCGC	3 cut(s)	577, 783, 1087
SspDI	GGCGCC	1 cut(s)	634
SspMI	CTAG	1 cut(s)	117
StyD4I	CCNGG	1 cut(s)	1249
StyI	CCWWGG	2 cut(s)	600, 683
TaaI	ACNGT	2 cut(s)	299, 589
TaiI	ACGT	2 cut(s)	376, 771
TaqI	TCGA	4 cut(s)	43, 302, 534, 897
TasI	AATT	6 cut(s)	150, 623, 655, 842, 1222, 1275
TatI	WGTACW	2 cut(s)	419, 1111
TauI	GCSGC	1 cut(s)	786
TfiI	GAWTC	7 cut(s)	224, 380, 491, 536, 707, 1032, 1270
Tru1I	TTAA	5 cut(s)	153, 218, 453, 902, 1116
Tru9I	TTAA	5 cut(s)	153, 218, 453, 902, 1116
TscAI	CASTG	1 cut(s)	274
TseI	GCWGC	1 cut(s)	1216
TspDTI	ATGAA	7 cut(s)	47, 678, 852, 1007, 1037, 1109, 1212
TspRI	CASTG	1 cut(s)	274
Vha464I	CTTAAG	1 cut(s)	452
XapI	RAATTY	2 cut(s)	623, 1275
XceI	RCATGY	1 cut(s)	725
XmiI	GTMKAC	2 cut(s)	897, 1234
XmnI	GAANNNNTTC	2 cut(s)	998, 1045
XspI	CTAG	1 cut(s)	117
ZraI	GACGTC	1 cut(s)	374
ZrmI	AGTACT	1 cut(s)	1113

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.