Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0014359

Physical Location & Seq

Genomic Coordinates Forward (+)

25205465 .. 25207781

Gene Structure 2317 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1171500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1299 bp

ATGAGCTTCCCTCCATCTACTGCATCTGGCGCAATGAAAGCGTTTCGCGGAAAGCTACATTTTCCTATTATCATGGTCTGCGCTATCGCCTTCATCAGTCTCCTCTACACCGAACGGATTAGCATCATTCTTTCCTCCAACCCTTTTTTCAAAGTTAAATCATGTTCCAAAACCAATGCCACTTCGAAAAATAAAAATAGAAATGCTACACTGGATAAGTTGAGGGATACCATATTAGATGATAGGTTTGAGTTCGACCCTGAGGAGTGCAGTATTGAGAACGGAAAGTGGGTGTTCAACCGTTCGATCAAGCCTCTTTACTCTGATAGAAGTTGCCCGTATCTTGATAGGCAAGTTTCATGTGTCAAGAATGGACGTCCGGATTCTGGCTATCGCTACTGGCAATGGCAGATCCAAGATTGCACATTGCCAAGATTTAATCCAGAAGTTGTTCTTAAGAAACTTAGCGGAAAGAGGCTACTTTTTGTTGGGGATTCTTTGCAAAGAGGTCAATGGCAATCTCTAGTCTGTATGGTTGAATCCATCATACCAGAGGACCAAAAGTCCATGCGACGCGGGCACTCTCACACAGTCTTCAGAGCCAAGGAGTATAATGCAAGTATTGAATTCTACTGGGCTCCATTTCTCGTTGATTCCAATTCTGATGAACATATAATCGGAAATCCAAAGGAAAGAATACTAAAAGTGGATTCAGTTGCCAAGCATGCCAAATACTGGACAGGAGTTGACATCCTTGTGTTCAACACTTACGTTTGGTGGATGAGCGGCTACACGATCAAATCATACTGGGGCTCATTTCCAAACGGAGAAGAAGGGTACGAAGAATTGGAAGCACCTGCTGCCTACAGAATAGCATTGAAGACATGGGCTAACTGGGTCGACACAAATGTGAATCCGAACAAGACCCGTGTTTTTTTCACTACTATGTCCCCTACACACATGAGAAGTGCAGACTGGGAAAATCCAGATGGGATGCGATGCTTCAACGAAACAAAGCCATACATGAAAAAGGGATTCTGGGGAACTGGTTCGGACAAGAAGATCATGCGCGTGGTGTCCGAAGTAATAAACAAAATGAAATGTCCGGTTTCAGTACTCAATGTAACGCAGATGTCAAATTACAGAGTTGATGCTCACACAAAAGTGTACACCGAAACCGGAGGCAATGTACTAACTGATGAGCAAAAGGCTGATGTGCTGCACAATTCAGATTGCATACATTGGTGCCTTCCTGGAGTTCCAGACACATGGAATGAAATTTTTGTGGCACATTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005886 GO:0005975 GO:0005976 GO:0006073 GO:0008150 GO:0008152 GO:0008374 GO:0009058 GO:0009059 GO:0009250 GO:0009664 GO:0009827 GO:0009987 GO:0010383 GO:0010393 GO:0010410 GO:0012505 GO:0016020 GO:0016043 GO:0016051 GO:0016407 GO:0016413 GO:0016740 GO:0016746 GO:0016747 GO:0017144 GO:0030243 GO:0030244 GO:0033692 GO:0034637 GO:0034645 GO:0042545 GO:0042546 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044036 GO:0044038 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0045229 GO:0045488 GO:0045489 GO:0045491 GO:0045492 GO:0051273 GO:0051274 GO:0070589 GO:0070592 GO:0071554 GO:0071555 GO:0071669 GO:0071704 GO:0071840 GO:0071944 GO:1901576 GO:1990538

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PC-Esterase PMR5N

Protein Analysis

433

Amino Acids

49.77

Weight (kDa)

8.79

Isoelectric Point (pI)

33.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMR5N	PF14416	88 - 141	1.2e-18	PMR5 N terminal Domain
PC-Esterase	PF13839	143 - 430	7e-95	GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014359)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G01360 AT5G01360
fragaria_vesca	FvH4_6g11230 FvH4_6g11230
malus_domestica	MD00G1172500.v1.1 MD12G1171500.v1.1
prunus_persica	Prupe.6G279700_v2.0.a1
pyrus_communis	pycom04g14210 pycom12g16280
rosa_chinensis	RchiOBHm_Chr3g0462741
rosa_laevigata	RLG00000024836
rosa_multiflora	Rmu_sc0000766.1_g000003 Rmu_sc0011057.1_g000007
rosa_roxburghii	Rroxscaffold_6G00417240
rosa_rugosa	Rorug03G0058900
rosa_samantha	Rh3DG122300
rosa_wichuraiana	Rw3G009900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	865
AatII	GACGTC	1 cut(s)	379
Acc36I	ACCTGC	1 cut(s)	865
AccB1I	GGYRCC	1 cut(s)	1245
AccB7I	CCANNNNNTGG	1 cut(s)	735
AccBSI	CCGCTC	1 cut(s)	786
AccI	GTMKAC	1 cut(s)	900
AccII	CGCG	3 cut(s)	48, 576, 1071
AccIII	TCCGGA	1 cut(s)	379
AciI	CCGC	4 cut(s)	48, 468, 576, 786
AclWI	GGATC	1 cut(s)	406
AcsI	RAATTY	2 cut(s)	626, 1278
AcuI	CTGAAG	1 cut(s)	580
AcyI	GRCGYC	1 cut(s)	376
AfaI	GTAC	4 cut(s)	839, 1116, 1169, 1191
AfiI	CCNNNNNNNGG	2 cut(s)	386, 735
AflII	CTTAAG	1 cut(s)	455
AgsI	TTSAA	7 cut(s)	151, 298, 539, 626, 763, 880, 1006
AhdI	GACNNNNNGTC	1 cut(s)	562
AjnI	CCWGG	1 cut(s)	1252
AjuI	GAANNNNNNNTTGG	2 cut(s)	131, 163
AleI	CACNNNNGTG	2 cut(s)	908, 1163
AluBI	AGCT	2 cut(s)	6, 55
AluI	AGCT	2 cut(s)	6, 55
Alw26I	GTCTC	1 cut(s)	104
AlwI	GGATC	1 cut(s)	406
Aor13HI	TCCGGA	1 cut(s)	379
ApeKI	GCWGC	2 cut(s)	860, 1219
ApoI	RAATTY	2 cut(s)	626, 1278
Asp700I	GAANNNNTTC	2 cut(s)	450, 1048
AspLEI	GCGC	3 cut(s)	32, 83, 1071
AspS9I	GGNCC	1 cut(s)	556
AsuII	TTCGAA	1 cut(s)	185
AvaII	GGWCC	1 cut(s)	556
AxyI	CCTNAGG	1 cut(s)	261
BaeGI	GKGCMC	1 cut(s)	582
BanI	GGYRCC	1 cut(s)	1245
BanII	GRGCYC	2 cut(s)	640, 815
BbsI	GAAGAC	2 cut(s)	586, 887
BbvI	GCAGC	2 cut(s)	847, 1206
BccI	CCATC	3 cut(s)	22, 551, 983
BciT130I	CCWGG	1 cut(s)	1254
BciVI	GTATCC	1 cut(s)	220
BcoDI	GTCTC	1 cut(s)	104
BfaI	CTAG	1 cut(s)	524
BfmI	CTRYAG	1 cut(s)	865
BfrI	CTTAAG	1 cut(s)	455
BfuAI	ACCTGC	1 cut(s)	865
BfuI	GTATCC	1 cut(s)	220
BisI	GCNGC	3 cut(s)	787, 861, 1220
BlsI	GCNGC	3 cut(s)	788, 862, 1221
BmcAI	AGTACT	1 cut(s)	1116
Bme1390I	CCNGG	1 cut(s)	1254
Bme18I	GGWCC	1 cut(s)	556
BmeRI	GACNNNNNGTC	1 cut(s)	562
BmgT120I	GGNCC	1 cut(s)	556
BmiI	GGNNCC	2 cut(s)	639, 1247
BmrFI	CCNGG	1 cut(s)	1254
BmrI	ACTGGG	4 cut(s)	643, 817, 904, 985
BmsI	GCATC	5 cut(s)	32, 132, 984, 989, 1141
BmuI	ACTGGG	4 cut(s)	643, 817, 904, 985
BpiI	GAAGAC	2 cut(s)	586, 887
BplI	GAGNNNNNCTC	1 cut(s)	27
BpmI	CTGGAG	1 cut(s)	1275
Bpu14I	TTCGAA	1 cut(s)	185
BsaBI	GATNNNNATC	1 cut(s)	122
BsaHI	GRCGYC	1 cut(s)	376
BsaJI	CCNNGG	1 cut(s)	603
BsaWI	WCCGGW	3 cut(s)	379, 1105, 1178
BsaXI	ACNNNNNCTCC	2 cut(s)	1173, 1203
Bsc4I	CCNNNNNNNGG	2 cut(s)	386, 735
Bse1I	ACTGG	8 cut(s)	216, 404, 638, 740, 812, 899, 980, 1051
Bse21I	CCTNAGG	1 cut(s)	261
Bse3DI	GCAATG	4 cut(s)	39, 410, 425, 1192
Bse8I	GATNNNNATC	1 cut(s)	122
BseAI	TCCGGA	1 cut(s)	379
BseBI	CCWGG	1 cut(s)	1254
BseDI	CCNNGG	1 cut(s)	603
BseGI	GGATG	3 cut(s)	750, 786, 999
BseJI	GATNNNNATC	1 cut(s)	122
BseLI	CCNNNNNNNGG	2 cut(s)	386, 735
BseMI	GCAATG	4 cut(s)	39, 410, 425, 1192
BseMII	CTCAG	1 cut(s)	252
BseNI	ACTGG	8 cut(s)	216, 404, 638, 740, 812, 899, 980, 1051
BseRI	GAGGAG	2 cut(s)	92, 278
BseSI	GKGCMC	1 cut(s)	582
BseXI	GCAGC	2 cut(s)	847, 1206
BsgI	GTGCAG	3 cut(s)	289, 990, 1205
Bsh1236I	CGCG	3 cut(s)	48, 576, 1071
BshNI	GGYRCC	1 cut(s)	1245
BsiSI	CCGG	3 cut(s)	380, 1106, 1179
BslFI	GGGAC	1 cut(s)	934
BslI	CCNNNNNNNGG	2 cut(s)	386, 735
BsmAI	GTCTC	1 cut(s)	104
BsmFI	GGGAC	1 cut(s)	934
Bsp119I	TTCGAA	1 cut(s)	185
Bsp1286I	GDGCHC	3 cut(s)	582, 640, 815
Bsp13I	TCCGGA	1 cut(s)	379
Bsp1407I	TGTACA	1 cut(s)	1167
Bsp143I	GATC	4 cut(s)	306, 411, 795, 1062
BspACI	CCGC	4 cut(s)	48, 468, 576, 786
BspCNI	CTCAG	1 cut(s)	253
BspEI	TCCGGA	1 cut(s)	379
BspFNI	CGCG	3 cut(s)	48, 576, 1071
BspLI	GGNNCC	2 cut(s)	639, 1247
BspMI	ACCTGC	1 cut(s)	865
BspPI	GGATC	1 cut(s)	406
BspT104I	TTCGAA	1 cut(s)	185
BspT107I	GGYRCC	1 cut(s)	1245
BspTI	CTTAAG	1 cut(s)	455
BsrBI	CCGCTC	1 cut(s)	786
BsrDI	GCAATG	4 cut(s)	39, 410, 425, 1192
BsrGI	TGTACA	1 cut(s)	1167
BsrI	ACTGG	8 cut(s)	216, 404, 638, 740, 812, 899, 980, 1051
BssECI	CCNNGG	1 cut(s)	603
BssMI	GATC	4 cut(s)	306, 411, 795, 1062
BssNI	GRCGYC	1 cut(s)	376
BssT1I	CCWWGG	1 cut(s)	603
Bst2UI	CCWGG	1 cut(s)	1254
Bst4CI	ACNGT	2 cut(s)	302, 592
BstACI	GRCGYC	1 cut(s)	376
BstAFI	CTTAAG	1 cut(s)	455
BstAPI	GCANNNNNTGC	1 cut(s)	860
BstAUI	TGTACA	1 cut(s)	1167
BstBI	TTCGAA	1 cut(s)	185
BstC8I	GCNNGC	2 cut(s)	578, 726
BstDEI	CTNAG	2 cut(s)	261, 464
BstF5I	GGATG	3 cut(s)	750, 786, 999
BstFNI	CGCG	3 cut(s)	48, 576, 1071
BstHHI	GCGC	3 cut(s)	32, 83, 1071
BstKTI	GATC	4 cut(s)	309, 414, 798, 1065
BstMAI	GTCTC	1 cut(s)	104
BstMBI	GATC	4 cut(s)	306, 411, 795, 1062
BstMWI	GCNNNNNNNGC	5 cut(s)	29, 38, 577, 725, 860
BstNI	CCWGG	1 cut(s)	1254
BstNSI	RCATGY	1 cut(s)	728
BstSCI	CCNGG	1 cut(s)	1252
BstSFI	CTRYAG	1 cut(s)	865
BstSLI	GKGCMC	1 cut(s)	582
BstUI	CGCG	3 cut(s)	48, 576, 1071
BstV1I	GCAGC	2 cut(s)	847, 1206
BstV2I	GAAGAC	2 cut(s)	586, 887
BstX2I	RGATCY	1 cut(s)	411
BstXI	CCANNNNNNTGG	1 cut(s)	1269
BstYI	RGATCY	1 cut(s)	411
Bsu36I	CCTNAGG	1 cut(s)	261
BsuI	GTATCC	1 cut(s)	220
BtgZI	GCGATG	1 cut(s)	1012
BtsCI	GGATG	3 cut(s)	750, 786, 999
BtsIMutI	CAGTG	1 cut(s)	209
BveI	ACCTGC	1 cut(s)	865
Cac8I	GCNNGC	2 cut(s)	578, 726
CfoI	GCGC	3 cut(s)	32, 83, 1071
Cfr13I	GGNCC	1 cut(s)	556
CseI	GACGC	1 cut(s)	582
Csp6I	GTAC	4 cut(s)	838, 1115, 1168, 1190
CviQI	GTAC	4 cut(s)	838, 1115, 1168, 1190
DdeI	CTNAG	2 cut(s)	261, 464
DpnI	GATC	4 cut(s)	308, 413, 797, 1064
DpnII	GATC	4 cut(s)	306, 411, 795, 1062
DriI	GACNNNNNGTC	1 cut(s)	562
Eam1105I	GACNNNNNGTC	1 cut(s)	562
Eco130I	CCWWGG	1 cut(s)	603
Eco24I	GRGCYC	2 cut(s)	640, 815
Eco47I	GGWCC	1 cut(s)	556
Eco57I	CTGAAG	1 cut(s)	580
Eco81I	CCTNAGG	1 cut(s)	261
EcoRI	GAATTC	1 cut(s)	626
EcoRII	CCWGG	1 cut(s)	1252
EcoT14I	CCWWGG	1 cut(s)	603
EcoT38I	GRGCYC	2 cut(s)	640, 815
ErhI	CCWWGG	1 cut(s)	603
FalI	AAGNNNNNCTT	2 cut(s)	438, 470
FaqI	GGGAC	1 cut(s)	934
FauI	CCCGC	1 cut(s)	569
FblI	GTMKAC	1 cut(s)	900
Fnu4HI	GCNGC	3 cut(s)	787, 861, 1220
FokI	GGATG	3 cut(s)	737, 793, 1006
FriOI	GRGCYC	2 cut(s)	640, 815
Fsp4HI	GCNGC	3 cut(s)	787, 861, 1220
FspBI	CTAG	1 cut(s)	524
GlaI	GCGC	3 cut(s)	31, 82, 1070
GluI	GCNGC	3 cut(s)	787, 861, 1220
GsuI	CTGGAG	1 cut(s)	1275
HapII	CCGG	3 cut(s)	380, 1106, 1179
HgaI	GACGC	1 cut(s)	582
HhaI	GCGC	3 cut(s)	32, 83, 1071
Hin1I	GRCGYC	1 cut(s)	376
Hin6I	GCGC	3 cut(s)	30, 81, 1069
HinP1I	GCGC	3 cut(s)	30, 81, 1069
HincII	GTYRAC	2 cut(s)	748, 901
HindII	GTYRAC	2 cut(s)	748, 901
HinfI	GANTC	7 cut(s)	383, 494, 539, 653, 710, 913, 1035
HpaII	CCGG	3 cut(s)	380, 1106, 1179
Hpy166II	GTNNAC	4 cut(s)	748, 901, 1168, 1170
Hpy188I	TCNGA	8 cut(s)	325, 599, 664, 680, 918, 1054, 1081, 1231
Hpy188III	TCNNGA	6 cut(s)	344, 367, 380, 443, 986, 1262
Hpy8I	GTNNAC	4 cut(s)	748, 901, 1168, 1170
Hpy99I	CGWCG	1 cut(s)	576
HpyAV	CCTTC	3 cut(s)	100, 827, 1259
HpyCH4III	ACNGT	2 cut(s)	302, 592
HpyCH4IV	ACGT	2 cut(s)	376, 771
HpyCH4V	TGCA	8 cut(s)	23, 270, 423, 502, 617, 971, 1222, 1236
HpyF10VI	GCNNNNNNNGC	5 cut(s)	29, 38, 577, 725, 860
HpyF3I	CTNAG	2 cut(s)	261, 464
HpySE526I	ACGT	2 cut(s)	376, 771
Hsp92I	GRCGYC	1 cut(s)	376
HspAI	GCGC	3 cut(s)	30, 81, 1069
Kpn2I	TCCGGA	1 cut(s)	379
Kzo9I	GATC	4 cut(s)	306, 411, 795, 1062
LmnI	GCTCC	1 cut(s)	643
Lsp1109I	GCAGC	2 cut(s)	847, 1206
LweI	GCATC	5 cut(s)	32, 132, 984, 989, 1141
MaeI	CTAG	1 cut(s)	524
MaeII	ACGT	2 cut(s)	376, 771
MaeIII	GTNAC	1 cut(s)	1123
MalI	GATC	4 cut(s)	308, 413, 797, 1064
MbiI	CCGCTC	1 cut(s)	786
MboI	GATC	4 cut(s)	306, 411, 795, 1062
MboII	GAAGA	5 cut(s)	586, 842, 854, 892, 1072
MflI	RGATCY	1 cut(s)	411
MhlI	GDGCHC	3 cut(s)	582, 640, 815
MluCI	AATT	6 cut(s)	626, 658, 845, 1138, 1225, 1278
MmeI	TCCRAC	1 cut(s)	162
MroI	TCCGGA	1 cut(s)	379
MroXI	GAANNNNTTC	2 cut(s)	450, 1048
MseI	TTAA	3 cut(s)	156, 438, 456
MslI	CAYNNNNRTG	5 cut(s)	755, 908, 944, 1070, 1163
MspCI	CTTAAG	1 cut(s)	455
MspI	CCGG	3 cut(s)	380, 1106, 1179
MspR9I	CCNGG	1 cut(s)	1254
MvaI	CCWGG	1 cut(s)	1254
MvnI	CGCG	3 cut(s)	48, 576, 1071
MwoI	GCNNNNNNNGC	5 cut(s)	29, 38, 577, 725, 860
NdeII	GATC	4 cut(s)	306, 411, 795, 1062
NlaIV	GGNNCC	2 cut(s)	639, 1247
NspI	RCATGY	1 cut(s)	728
NspV	TTCGAA	1 cut(s)	185
OliI	CACNNNNGTG	2 cut(s)	908, 1163
PaeI	GCATGC	1 cut(s)	728
PaqCI	CACCTGC	1 cut(s)	865
PdmI	GAANNNNTTC	2 cut(s)	450, 1048
PfeI	GAWTC	7 cut(s)	383, 494, 539, 653, 710, 913, 1035
PflMI	CCANNNNNTGG	1 cut(s)	735
PfoI	TCCNGGA	1 cut(s)	1252
PkrI	GCNGC	3 cut(s)	788, 862, 1221
Psp6I	CCWGG	1 cut(s)	1252
PspGI	CCWGG	1 cut(s)	1252
PspN4I	GGNNCC	2 cut(s)	639, 1247
PspPI	GGNCC	1 cut(s)	556
PsuI	RGATCY	1 cut(s)	411
RsaI	GTAC	4 cut(s)	839, 1116, 1169, 1191
RsaNI	GTAC	4 cut(s)	838, 1115, 1168, 1190
RseI	CAYNNNNRTG	5 cut(s)	755, 908, 944, 1070, 1163
SalI	GTCGAC	1 cut(s)	899
SaqAI	TTAA	3 cut(s)	156, 438, 456
SatI	GCNGC	3 cut(s)	787, 861, 1220
Sau3AI	GATC	4 cut(s)	306, 411, 795, 1062
Sau96I	GGNCC	1 cut(s)	556
ScaI	AGTACT	1 cut(s)	1116
ScrFI	CCNGG	1 cut(s)	1254
SduI	GDGCHC	3 cut(s)	582, 640, 815
SetI	ASST	7 cut(s)	8, 57, 248, 379, 511, 774, 859
SfaNI	GCATC	5 cut(s)	32, 132, 984, 989, 1141
SfcI	CTRYAG	1 cut(s)	865
SfuI	TTCGAA	1 cut(s)	185
SinI	GGWCC	1 cut(s)	556
SmiMI	CAYNNNNRTG	5 cut(s)	755, 908, 944, 1070, 1163
SmlI	CTYRAG	1 cut(s)	455
SmoI	CTYRAG	1 cut(s)	455
SphI	GCATGC	1 cut(s)	728
Sse9I	AATT	6 cut(s)	626, 658, 845, 1138, 1225, 1278
SsiI	CCGC	4 cut(s)	48, 468, 576, 786
SspMI	CTAG	1 cut(s)	524
StyD4I	CCNGG	1 cut(s)	1252
StyI	CCWWGG	1 cut(s)	603
TaaI	ACNGT	2 cut(s)	302, 592
TaiI	ACGT	2 cut(s)	379, 774
TaqI	TCGA	4 cut(s)	185, 255, 305, 900
TasI	AATT	6 cut(s)	626, 658, 845, 1138, 1225, 1278
TatI	WGTACW	3 cut(s)	1114, 1167, 1189
TauI	GCSGC	1 cut(s)	789
TfiI	GAWTC	7 cut(s)	383, 494, 539, 653, 710, 913, 1035
Tru1I	TTAA	3 cut(s)	156, 438, 456
Tru9I	TTAA	3 cut(s)	156, 438, 456
TscAI	CASTG	1 cut(s)	216
TseI	GCWGC	2 cut(s)	860, 1219
TspDTI	ATGAA	7 cut(s)	50, 82, 348, 681, 1040, 1112, 1290
TspGWI	ACGGA	3 cut(s)	130, 297, 840
TspRI	CASTG	1 cut(s)	216
Van91I	CCANNNNNTGG	1 cut(s)	735
Vha464I	CTTAAG	1 cut(s)	455
VpaK11BI	GGWCC	1 cut(s)	556
XapI	RAATTY	2 cut(s)	626, 1278
XceI	RCATGY	1 cut(s)	728
XmiI	GTMKAC	1 cut(s)	900
XmnI	GAANNNNTTC	2 cut(s)	450, 1048
XspI	CTAG	1 cut(s)	524
ZraI	GACGTC	1 cut(s)	377
ZrmI	AGTACT	1 cut(s)	1116

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.